AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0537210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0537210 MtrunA17_Chr4g0011791 99.214 509 4 0 1 509 1 509 0.0 1046
Msa0537210 MtrunA17_Chr4g0011771 73.643 516 126 6 1 509 1 513 0.0 778
Msa0537210 MtrunA17_Chr8g0391311 49.625 534 228 13 7 509 18 541 4.15e-177 509
Msa0537210 MtrunA17_Chr8g0391291 49.625 534 228 13 7 509 16 539 8.98e-177 508
Msa0537210 MtrunA17_Chr4g0018371 50.297 505 226 10 29 509 36 539 2.07e-170 491
Msa0537210 MtrunA17_Chr3g0090091 50.099 507 229 11 23 509 26 528 6.38e-167 482
Msa0537210 MtrunA17_Chr3g0079361 49.143 525 239 14 9 509 7 527 5.41e-164 475
Msa0537210 MtrunA17_Chr3g0079341 48.015 529 246 13 6 509 3 527 7.34e-164 474
Msa0537210 MtrunA17_Chr3g0079351 49.605 506 232 12 23 509 26 527 7.28e-163 472
Msa0537210 MtrunA17_Chr7g0269541 47.156 545 247 13 1 505 9 552 2.49e-160 466
Msa0537210 MtrunA17_Chr7g0233771 46.589 513 243 11 24 509 46 554 4.07e-147 432
Msa0537210 MtrunA17_Chr8g0345281 46.679 527 230 14 29 509 68 589 1.11e-145 430
Msa0537210 MtrunA17_Chr4g0011751 51.972 431 185 9 95 509 65 489 2.73e-144 423
Msa0537210 MtrunA17_Chr7g0233831 44.712 539 256 15 1 509 29 555 1.03e-142 421
Msa0537210 MtrunA17_Chr7g0272141 49.443 449 189 10 91 509 102 542 2.47e-142 420
Msa0537210 MtrunA17_Chr4g0069841 51.025 439 183 9 95 509 86 516 3.31e-141 416
Msa0537210 MtrunA17_Chr1g0192331 46.604 530 245 14 6 509 11 528 5.66e-141 416
Msa0537210 MtrunA17_Chr3g0126771 43.321 524 252 11 28 509 77 597 3.44e-140 417
Msa0537210 MtrunA17_Chr1g0195301 46.241 532 223 16 33 509 32 555 4.59e-139 412
Msa0537210 MtrunA17_Chr1g0195321 46.241 532 223 16 33 509 32 555 4.59e-139 412
Msa0537210 MtrunA17_Chr2g0302021 50.000 438 192 8 95 509 92 525 6.83e-139 410
Msa0537210 MtrunA17_Chr1g0195331 46.429 532 222 16 33 509 32 555 1.13e-138 411
Msa0537210 MtrunA17_Chr1g0175661 50.108 465 180 14 95 509 96 558 2.00e-137 408
Msa0537210 MtrunA17_Chr1g0147051 42.115 539 263 12 18 509 71 607 3.19e-137 409
Msa0537210 MtrunA17_Chr7g0233801 44.767 516 247 14 24 509 46 553 3.73e-137 407
Msa0537210 MtrunA17_Chr7g0269531 45.094 530 231 15 33 509 43 565 1.03e-134 401
Msa0537210 MtrunA17_Chr1g0195351 45.865 532 223 17 33 509 32 553 1.29e-134 400
Msa0537210 MtrunA17_Chr3g0080391 44.660 515 244 12 27 509 69 574 5.53e-134 400
Msa0537210 MtrunA17_Chr8g0354901 45.898 512 244 15 27 509 74 581 8.76e-132 394
Msa0537210 MtrunA17_Chr7g0269511 44.925 532 229 16 33 509 42 564 2.50e-131 393
Msa0537210 MtrunA17_Chr7g0269521 43.609 532 242 13 30 509 37 562 1.11e-128 386
Msa0537210 MtrunA17_Chr8g0354911 43.144 547 229 15 33 509 78 612 1.06e-127 385
Msa0537210 MtrunA17_Chr4g0069881 43.504 508 254 14 26 509 37 535 3.38e-127 381
Msa0537210 MtrunA17_Chr4g0069861 45.475 442 207 8 95 509 83 517 3.15e-125 375
Msa0537210 MtrunA17_Chr4g0069891 40.762 525 267 13 23 509 75 593 1.58e-122 371
Msa0537210 MtrunA17_Chr1g0195311 64.639 263 89 3 250 509 53 314 1.73e-120 356
Msa0537210 MtrunA17_Chr3g0080381 41.805 543 244 17 33 509 92 628 2.03e-117 359
Msa0537210 MtrunA17_Chr1g0194091 40.598 468 229 10 90 509 103 569 7.31e-112 343
Msa0537210 MtrunA17_Chr1g0192321 38.202 534 277 14 25 508 52 582 7.14e-111 341
Msa0537210 MtrunA17_Chr8g0345211 39.062 512 279 11 27 509 75 582 4.80e-107 331
Msa0537210 MtrunA17_Chr1g0192311 35.484 527 280 13 25 505 54 566 3.37e-104 323
Msa0537210 MtrunA17_Chr1g0192281 35.484 527 280 13 25 505 54 566 3.37e-104 323
Msa0537210 MtrunA17_Chr7g0272151 37.218 532 282 16 25 508 55 582 5.89e-103 320
Msa0537210 MtrunA17_Chr3g0129921 37.273 550 290 17 7 508 21 563 5.44e-102 317
Msa0537210 MtrunA17_Chr1g0194081 38.415 492 260 13 59 509 74 563 1.90e-101 316
Msa0537210 MtrunA17_Chr8g0384121 38.672 512 262 14 27 498 54 553 5.61e-101 315
Msa0537210 MtrunA17_Chr7g0270691 37.620 521 275 16 29 509 39 549 1.18e-99 311
Msa0537210 MtrunA17_Chr7g0270661 39.485 466 226 10 93 504 106 569 4.75e-99 310
Msa0537210 MtrunA17_Chr7g0270671 39.485 466 226 10 93 504 106 569 4.75e-99 310
Msa0537210 MtrunA17_Chr1g0192301 34.496 516 300 12 25 505 59 571 1.64e-98 309
Msa0537210 MtrunA17_Chr4g0000121 34.156 527 287 13 25 505 41 553 7.17e-98 306
Msa0537210 MtrunA17_Chr7g0270651 37.580 471 238 11 93 509 106 574 2.09e-96 303
Msa0537210 MtrunA17_Chr7g0234711 61.842 228 83 3 286 509 42 269 3.49e-96 292
Msa0537210 MtrunA17_Chr2g0302001 47.059 323 159 6 191 509 123 437 5.15e-95 295
Msa0537210 MtrunA17_Chr4g0067611 34.733 524 295 14 25 508 55 571 5.68e-92 291
Msa0537210 MtrunA17_Chr2g0301931 48.936 282 138 5 231 509 14 292 9.31e-91 279
Msa0537210 MtrunA17_Chr2g0295551 34.942 518 297 14 25 508 52 563 2.03e-90 287
Msa0537210 MtrunA17_Chr4g0067601 34.357 521 293 13 29 508 59 571 1.08e-89 286
Msa0537210 MtrunA17_Chr7g0270631 37.613 444 230 8 93 489 94 537 1.56e-88 294
Msa0537210 MtrunA17_Chr7g0270631 36.264 455 225 11 93 487 642 1091 1.06e-76 261
Msa0537210 MtrunA17_Chr2g0295531 32.258 527 326 12 7 506 21 543 3.83e-88 283
Msa0537210 MtrunA17_Chr2g0295541 33.151 549 296 15 25 508 52 594 1.20e-84 273
Msa0537210 MtrunA17_Chr8g0380581 43.620 337 170 10 182 506 45 373 2.14e-83 263
Msa0537210 MtrunA17_Chr5g0437831 40.469 341 190 8 179 509 46 383 2.10e-77 248
Msa0537210 MtrunA17_Chr7g0268241 40.248 323 177 7 193 509 38 350 2.15e-71 231
Msa0537210 MtrunA17_Chr2g0334111 40.385 312 168 7 204 509 24 323 1.36e-70 228
Msa0537210 MtrunA17_Chr7g0271381 30.350 514 316 16 25 509 56 556 7.51e-68 228
Msa0537210 MtrunA17_Chr8g0391261 63.971 136 47 1 376 509 31 166 5.22e-57 187
Msa0537210 MtrunA17_Chr7g0234731 40.146 274 145 9 23 281 45 314 2.05e-54 185
Msa0537210 MtrunA17_Chr6g0487101 39.735 302 152 9 213 498 93 380 2.37e-51 179
Msa0537210 MtrunA17_Chr8g0391281 35.974 303 156 11 7 281 16 308 8.69e-51 176
Msa0537210 MtrunA17_Chr8g0389771 37.419 310 164 9 206 498 81 377 1.73e-49 174
Msa0537210 MtrunA17_Chr6g0453551 35.644 303 167 9 205 492 6 295 1.80e-49 172
Msa0537210 MtrunA17_Chr6g0466951 34.296 277 159 7 203 471 67 328 2.02e-45 162
Msa0537210 MtrunA17_Chr7g0217941 34.448 299 172 7 207 492 8 295 3.64e-45 160
Msa0537210 MtrunA17_Chr2g0299431 42.918 233 126 5 179 406 28 258 1.02e-44 159
Msa0537210 MtrunA17_Chr1g0194311 32.919 322 189 8 190 498 61 368 3.05e-44 160
Msa0537210 MtrunA17_Chr7g0258341 32.143 336 196 9 177 498 70 387 1.13e-43 159
Msa0537210 MtrunA17_Chr6g0466961 34.545 275 157 6 205 471 40 299 4.19e-42 153
Msa0537210 MtrunA17_Chr2g0294321 32.063 315 191 8 194 498 45 346 5.82e-41 150
Msa0537210 MtrunA17_Chr1g0161731 30.892 314 186 8 200 502 44 337 5.49e-39 145
Msa0537210 MtrunA17_Chr5g0446301 33.225 307 170 8 211 498 67 357 1.14e-37 142
Msa0537210 MtrunA17_Chr4g0070051 31.889 323 185 11 193 498 39 343 4.55e-36 137
Msa0537210 MtrunA17_Chr2g0295851 32.131 305 173 11 205 498 55 336 1.56e-34 132
Msa0537210 MtrunA17_Chr5g0399221 29.808 312 192 8 205 501 63 362 3.67e-33 129
Msa0537210 MtrunA17_Chr5g0440871 29.530 298 192 6 205 500 39 320 1.06e-31 124
Msa0537210 MtrunA17_Chr7g0265201 30.492 305 183 7 207 500 101 387 1.21e-31 125
Msa0537210 MtrunA17_Chr8g0351871 27.869 305 194 6 205 498 33 322 1.97e-31 124
Msa0537210 MtrunA17_Chr4g0037751 29.063 320 193 11 194 498 61 361 2.08e-31 124
Msa0537210 MtrunA17_Chr5g0437821 42.424 132 72 2 315 442 4 135 7.82e-31 116
Msa0537210 MtrunA17_Chr6g0459781 37.853 177 99 4 198 363 6 182 1.51e-30 118
Msa0537210 MtrunA17_Chr5g0420831 28.280 343 218 10 171 498 32 361 1.23e-29 119
Msa0537210 MtrunA17_Chr4g0025841 31.687 243 147 6 205 443 37 264 7.68e-25 103
Msa0537210 MtrunA17_Chr5g0440901 35.762 151 88 3 294 443 1 143 2.69e-23 95.9
Msa0537210 MtrunA17_Chr2g0314691 26.415 212 140 4 290 498 3 201 1.80e-21 92.8
Msa0537210 MtrunA17_Chr7g0265161 31.461 178 92 4 267 439 5 157 3.33e-17 81.3
Msa0537210 MtrunA17_Chr6g0460141 36.567 134 75 3 232 355 5 138 5.08e-16 75.5
Msa0537210 MtrunA17_Chr5g0440891 32.692 156 93 5 205 356 48 195 1.81e-14 72.4
Msa0537210 MtrunA17_Chr6g0459791 37.398 123 66 4 377 498 4 116 4.09e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0537210 AT2G45220.1 66.926 514 161 6 1 509 2 511 0.0 710
Msa0537210 AT5G51490.1 52.381 504 210 10 32 509 37 536 5.71e-177 508
Msa0537210 AT5G51500.1 50.493 507 225 9 27 509 36 540 1.61e-167 484
Msa0537210 AT3G47400.1 49.016 508 229 10 32 509 87 594 6.53e-163 475
Msa0537210 AT4G00190.1 49.897 485 217 8 33 509 8 474 2.05e-162 469
Msa0537210 AT1G23200.1 46.324 544 230 13 19 509 20 554 3.71e-158 461
Msa0537210 AT3G49220.1 44.531 512 253 9 28 509 86 596 7.38e-142 421
Msa0537210 AT3G49220.3 44.531 512 253 9 28 509 131 641 1.83e-141 422
Msa0537210 AT5G53370.1 44.250 513 254 8 28 509 74 585 1.87e-141 420
Msa0537210 AT5G53370.2 44.250 513 254 8 28 509 110 621 2.93e-141 421
Msa0537210 AT3G59010.1 50.926 432 185 5 98 509 104 528 5.23e-141 417
Msa0537210 AT1G11580.1 46.154 507 251 10 25 509 51 557 7.54e-141 417
Msa0537210 AT1G11580.2 46.154 507 251 10 25 509 72 578 1.62e-140 417
Msa0537210 AT3G14310.1 44.732 541 240 13 25 509 55 592 2.38e-140 417
Msa0537210 AT1G53830.1 44.815 540 245 13 19 509 52 587 2.03e-138 412
Msa0537210 AT3G05620.1 46.091 486 219 11 57 509 66 541 2.56e-136 405
Msa0537210 AT3G43270.1 48.107 449 196 9 95 509 80 525 7.30e-136 403
Msa0537210 AT4G02320.1 47.439 449 207 10 89 509 71 518 7.16e-135 400
Msa0537210 AT3G60730.1 45.614 456 207 8 93 509 66 519 3.14e-132 394
Msa0537210 AT2G43050.1 49.061 426 194 9 98 509 100 516 4.72e-130 388
Msa0537210 AT4G33220.1 45.887 462 197 9 95 509 68 523 1.44e-129 387
Msa0537210 AT4G02330.1 41.667 576 252 19 6 509 8 571 1.63e-127 384
Msa0537210 AT4G02300.1 45.372 443 217 8 92 509 90 532 6.56e-127 380
Msa0537210 AT2G47550.1 49.460 463 185 13 94 509 98 558 7.71e-127 381
Msa0537210 AT1G02810.1 41.017 590 247 18 5 509 4 577 1.36e-125 379
Msa0537210 AT4G33220.2 54.491 334 144 5 182 509 71 402 4.21e-123 366
Msa0537210 AT2G26450.1 42.520 508 260 15 29 509 109 611 1.06e-122 372
Msa0537210 AT5G04960.1 40.275 509 262 12 28 507 67 562 4.28e-119 362
Msa0537210 AT3G05610.1 38.372 516 283 13 25 509 54 565 5.16e-119 365
Msa0537210 AT3G10710.1 41.992 512 259 13 23 509 63 561 1.27e-118 360
Msa0537210 AT5G20860.2 44.213 432 215 9 99 509 112 538 1.51e-117 357
Msa0537210 AT2G26440.1 40.310 516 272 11 27 509 35 547 1.42e-116 355
Msa0537210 AT3G10720.2 40.892 538 252 15 33 509 83 615 8.11e-116 355
Msa0537210 AT5G49180.1 40.504 516 260 17 24 505 64 566 6.99e-115 351
Msa0537210 AT5G27870.1 36.770 514 292 12 25 509 53 562 3.28e-113 352
Msa0537210 AT5G04970.1 39.889 539 256 15 33 509 88 620 6.16e-113 348
Msa0537210 AT4G33230.1 40.226 532 267 14 19 509 85 606 2.63e-112 345
Msa0537210 AT1G53840.1 38.462 520 268 14 27 503 71 581 2.42e-107 332
Msa0537210 AT1G11590.1 37.547 530 298 11 6 509 2 524 3.32e-107 330
Msa0537210 AT3G06830.1 38.855 489 248 14 62 507 86 566 4.34e-107 331
Msa0537210 AT4G03930.1 37.170 530 300 11 6 509 2 524 2.12e-105 325
Msa0537210 AT3G27980.1 43.035 402 203 6 124 509 106 497 3.87e-105 323
Msa0537210 AT3G14300.1 38.235 510 269 11 27 498 457 958 9.61e-104 332
Msa0537210 AT3G10720.1 57.308 260 107 3 253 509 1 259 1.37e-102 308
Msa0537210 AT4G15980.1 46.991 349 175 7 168 507 353 700 2.23e-100 317
Msa0537210 AT4G15980.2 46.991 349 175 7 168 507 363 710 3.94e-100 317
Msa0537210 AT5G64640.1 36.557 517 282 14 26 501 90 601 2.16e-97 306
Msa0537210 AT5G09760.1 36.739 509 279 14 27 497 43 546 1.31e-93 295
Msa0537210 AT5G09760.2 36.739 509 279 14 27 497 69 572 2.54e-93 295
Msa0537210 AT5G20860.1 40.704 398 183 8 99 475 112 477 9.78e-92 289
Msa0537210 AT2G47030.1 43.678 348 175 8 181 509 243 588 3.08e-82 267
Msa0537210 AT2G47040.1 43.478 345 177 8 181 509 253 595 2.78e-79 259
Msa0537210 AT3G62170.1 41.011 356 181 8 181 509 235 588 1.55e-77 254
Msa0537210 AT1G11370.1 48.780 246 120 4 202 441 43 288 2.54e-73 234
Msa0537210 AT5G19730.1 37.134 307 167 8 206 498 87 381 5.44e-48 171
Msa0537210 AT3G29090.1 35.789 285 162 5 200 471 2 278 4.79e-47 166
Msa0537210 AT3G17060.1 32.808 317 186 9 194 498 35 336 6.30e-44 159
Msa0537210 AT1G05310.1 33.038 339 195 9 174 498 68 388 8.28e-44 159
Msa0537210 AT2G36700.1 32.667 300 184 5 207 498 44 333 1.77e-43 157
Msa0537210 AT2G36710.1 32.704 318 185 10 202 503 86 390 2.54e-40 150
Msa0537210 AT1G44980.1 43.617 188 101 3 183 365 54 241 1.36e-39 144
Msa0537210 AT5G18990.1 34.146 287 163 7 200 471 24 299 3.18e-38 143
Msa0537210 AT2G21610.1 30.100 299 187 7 205 498 51 332 7.24e-38 142
Msa0537210 AT2G21610.2 30.100 299 187 7 205 498 56 337 7.97e-38 142
Msa0537210 AT5G55590.1 33.657 309 178 10 205 498 82 378 1.28e-36 139
Msa0537210 AT2G19150.1 31.889 323 190 10 197 505 33 339 3.58e-36 137
Msa0537210 AT3G24130.1 32.509 283 169 6 202 471 31 304 6.39e-36 137
Msa0537210 AT5G47500.1 28.267 329 198 8 185 498 52 357 2.41e-35 135
Msa0537210 AT5G26810.1 31.098 328 196 10 191 500 20 335 3.96e-35 134
Msa0537210 AT5G07430.1 28.610 374 232 12 140 498 2 355 4.39e-35 135
Msa0537210 AT5G55590.2 35.178 253 140 8 205 442 82 325 8.53e-34 130
Msa0537210 AT3G49220.2 30.961 281 166 7 28 281 131 410 2.10e-33 131
Msa0537210 AT2G47280.1 30.744 309 183 8 205 498 35 327 7.28e-31 122
Msa0537210 AT2G19150.2 32.558 258 151 8 197 442 33 279 3.44e-29 117
Msa0537210 AT5G07420.1 27.859 341 214 10 176 498 29 355 6.56e-29 117
Msa0537210 AT5G61680.1 27.885 312 200 9 198 498 61 358 3.01e-26 110
Msa0537210 AT5G07410.1 26.087 345 228 10 167 498 25 355 3.02e-26 110
Msa0537210 AT1G69940.1 25.946 370 244 12 144 498 1 355 2.16e-25 107

Find 123 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATTACTAGTTAGTTTAT+TGG 0.189679 4_1:+15160888 Msa0537210:three_prime_UTR
GGTTTGATTGAAAGCATTAT+TGG 0.202138 4_1:-15157635 None:intergenic
TATGAACAATTTAGTATATA+TGG 0.210410 4_1:-15160786 None:intergenic
GAATTATAATTCATAATTCT+AGG 0.221921 4_1:+15160344 Msa0537210:CDS
GGGACAGTTGATTTCATCTT+TGG 0.244433 4_1:+15160214 Msa0537210:CDS
TATAGAGAATGTAACATCTA+TGG 0.255655 4_1:+15160193 Msa0537210:CDS
GAGAAGGGTTTATGAGGTTT+TGG 0.290145 4_1:-15157431 None:intergenic
CAGGTTCATCAACTTCAAAT+AGG 0.301965 4_1:+15160557 Msa0537210:CDS
CTGGACTTTAAGCGTTTCTT+TGG 0.317020 4_1:+15160695 Msa0537210:exon
TTCATGAAAACTGTTCTTGA+TGG 0.322264 4_1:+15160448 Msa0537210:CDS
AAGCTGTACTAAGCCATGTT+TGG 0.344214 4_1:-15157864 None:intergenic
TTCAATGGCTTCATTTCCTT+TGG 0.346370 4_1:+15157492 Msa0537210:exon
TACTCACATGGTTGAGGATA+AGG 0.351224 4_1:-15157604 None:intergenic
CAAGCTGTTGCATTTCGTTC+CGG 0.356258 4_1:+15160091 Msa0537210:CDS
ACCGTTGGGAATTTCATTGC+CGG 0.366873 4_1:+15160634 Msa0537210:CDS
TATTGGCTGTTCTACTGTAT+AGG 0.369253 4_1:+15160905 Msa0537210:three_prime_UTR
GAATATGCTAATACAGGTTC+AGG 0.371500 4_1:+15160538 Msa0537210:CDS
AGCTGTACTAAGCCATGTTT+GGG 0.379379 4_1:-15157863 None:intergenic
ATTTCATTGCCGGTAATTCA+TGG 0.379520 4_1:+15160644 Msa0537210:CDS
CATCTTTGTAACTTGGTTGT+TGG 0.388751 4_1:-15158014 None:intergenic
GTTTCCTATCCATGAATTAC+CGG 0.393229 4_1:-15160653 None:intergenic
AACTTAGAGAAGGGTTTATG+AGG 0.395587 4_1:-15157437 None:intergenic
ACTCACATGGTTGAGGATAA+GGG 0.396273 4_1:-15157603 None:intergenic
ATGGTTGAGGATAAGGGGTT+TGG 0.402492 4_1:-15157597 None:intergenic
ACAATAGTGGAGAAAAGAAA+TGG 0.403364 4_1:-15157535 None:intergenic
AGGTTCATCAACTTCAAATA+GGG 0.410477 4_1:+15160558 Msa0537210:CDS
CGTCGTTGCTAAGGATGGTT+CGG 0.414368 4_1:+15158110 Msa0537210:CDS
TTGTAACTTGGTTGTTGGTA+AGG 0.414505 4_1:-15158009 None:intergenic
TTCTAGGGTTTCCGCTCAAT+CGG 0.419321 4_1:+15160360 Msa0537210:CDS
TTTGCAGCTGCTACTGGTGA+TGG 0.425142 4_1:+15160016 Msa0537210:intron
GTCGTTGCTAAGGATGGTTC+GGG 0.434190 4_1:+15158111 Msa0537210:CDS
AAATTGTTCATAAGATCAAT+TGG 0.436448 4_1:+15160797 Msa0537210:three_prime_UTR
TTATTTAAAAGAATTGTTGT+TGG 0.441698 4_1:+15160985 Msa0537210:three_prime_UTR
CATGGGTTAAACCTGGTGAT+AGG 0.443867 4_1:+15158046 Msa0537210:CDS
AAGCTTCACCTTTCACCGTT+GGG 0.443952 4_1:+15160620 Msa0537210:CDS
AACTTCTTCCGCGGCTTCGA+AGG 0.447444 4_1:+15158080 Msa0537210:CDS
CAAGCTTCACCTTTCACCGT+TGG 0.453573 4_1:+15160619 Msa0537210:CDS
ACGGGTGTGCCATTCACCTC+TGG 0.458761 4_1:+15160676 Msa0537210:CDS
GGCTAATGTCGTCGTTGCTA+AGG 0.459522 4_1:+15158101 Msa0537210:CDS
TATGCTGAATATGCTAATAC+AGG 0.469023 4_1:+15160532 Msa0537210:CDS
CAACTTCAAATAGGGTTACA+TGG 0.473864 4_1:+15160566 Msa0537210:CDS
ATGAAATTCCCAACGGTGAA+AGG 0.479388 4_1:-15160628 None:intergenic
CAACAACCAAGTTACAAAGA+TGG 0.480845 4_1:+15158015 Msa0537210:CDS
CCCTGCTGGTTGGTTACCAT+GGG 0.482029 4_1:+15160480 Msa0537210:CDS
TCCTTGCGCGGTAACTGTGA+TGG 0.482943 4_1:-15160296 None:intergenic
TCGTTCCGGCTCCGATCTAT+CGG 0.484411 4_1:+15160105 Msa0537210:CDS
AATTATAATTCATAATTCTA+GGG 0.485667 4_1:+15160345 Msa0537210:CDS
ATAGAGAATGTAACATCTAT+GGG 0.487737 4_1:+15160194 Msa0537210:CDS
TGAGTAACCATAAACAATAG+TGG 0.489662 4_1:-15157548 None:intergenic
GCAAAGAATGTCATGTTGGT+TGG 0.492347 4_1:+15158240 Msa0537210:CDS
TCTTGTGGGCTACGGCACTT+TGG 0.492867 4_1:-15157748 None:intergenic
GGAAATCCATCTTTGTAACT+TGG 0.499245 4_1:-15158021 None:intergenic
ATTGCCGGTAATTCATGGAT+AGG 0.500552 4_1:+15160649 Msa0537210:CDS
TCCCATGGTAACCAACCAGC+AGG 0.502238 4_1:-15160481 None:intergenic
AGTTTCACTTGAACTTGCAA+TGG 0.505619 4_1:+15157684 Msa0537210:CDS
ACCCTGCTGGTTGGTTACCA+TGG 0.509749 4_1:+15160479 Msa0537210:CDS
TAACTAGTAATACATTTGAA+TGG 0.513519 4_1:-15160879 None:intergenic
GGTACGATTTAACAGAGCTA+GGG 0.515662 4_1:-15160390 None:intergenic
GATGCTGCACCAAGTGGTAG+TGG 0.515780 4_1:+15158162 Msa0537210:CDS
CATTAAGTTCCATAAAATCA+TGG 0.516559 4_1:+15157569 Msa0537210:CDS
TCAAATAGGGTTACATGGAA+AGG 0.527062 4_1:+15160571 Msa0537210:CDS
AGGTACGATTTAACAGAGCT+AGG 0.534156 4_1:-15160391 None:intergenic
AAATAATACTCACATGGTTG+AGG 0.534999 4_1:-15157610 None:intergenic
ATATAAAGACACTAAAATCA+AGG 0.536961 4_1:+15157384 None:intergenic
ATTGGTAAAACCATAATCAC+TGG 0.538242 4_1:+15158270 Msa0537210:CDS
ATCACATTCAGAAACACTGC+TGG 0.539268 4_1:+15160055 Msa0537210:CDS
TCACCAGGTTTAACCCATGT+TGG 0.540325 4_1:-15158042 None:intergenic
TGAAGAAGTTTCCTATCACC+AGG 0.540939 4_1:-15158057 None:intergenic
ATGATGAGAAACTTAGAGAA+GGG 0.541566 4_1:-15157446 None:intergenic
ATCACTGGTAGCAAAAGTGT+TGG 0.542034 4_1:+15158285 Msa0537210:CDS
CTCACATGGTTGAGGATAAG+GGG 0.542503 4_1:-15157602 None:intergenic
ACCATCACAGTTACCGCGCA+AGG 0.543990 4_1:+15160295 Msa0537210:CDS
TTTGTAAAATACCGATAGAT+CGG 0.549087 4_1:-15160116 None:intergenic
TAAAGCAAAGAATGTCATGT+TGG 0.552893 4_1:+15158236 Msa0537210:CDS
ATGTTGGTTGGAGATGGTAT+TGG 0.553011 4_1:+15158252 Msa0537210:CDS
ACAAAGATGGATTTCCAACA+TGG 0.557740 4_1:+15158028 Msa0537210:CDS
AATGTCGTCGTTGCTAAGGA+TGG 0.559942 4_1:+15158105 Msa0537210:CDS
ACTGGTAGCAAAAGTGTTGG+AGG 0.563435 4_1:+15158288 Msa0537210:CDS
ATTAAAAGAGTTGTTACCAA+AGG 0.567706 4_1:-15157508 None:intergenic
ACCGATCCAAATCAAAACAC+TGG 0.569193 4_1:+15160322 Msa0537210:CDS
CACAATATGAATCCCAAACA+TGG 0.578929 4_1:+15157851 Msa0537210:CDS
GCTTAAAGTCCAGAGGTGAA+TGG 0.579277 4_1:-15160685 None:intergenic
AAATACCGATAGATCGGAGC+CGG 0.582499 4_1:-15160110 None:intergenic
TTTCCAACATGGGTTAAACC+TGG 0.589586 4_1:+15158039 Msa0537210:CDS
AATGTCATGTTGGTTGGAGA+TGG 0.590411 4_1:+15158246 Msa0537210:CDS
GTTACATTATTAGACAACAA+AGG 0.594044 4_1:-15157949 None:intergenic
ACTTTCCGTTCAGCAACCGT+TGG 0.594941 4_1:+15158321 Msa0537210:CDS
TATGATGAGAAACTTAGAGA+AGG 0.598833 4_1:-15157447 None:intergenic
TATGTGATATATGTGAAGGC+TGG 0.599695 4_1:+15158189 Msa0537210:CDS
GGGCAAGTACACGACGGTGA+AGG 0.602626 4_1:+15158131 Msa0537210:CDS
GCTGGTTGGTTACCATGGGA+TGG 0.604546 4_1:+15160484 Msa0537210:CDS
CTGCACCAAGTGGTAGTGGA+AGG 0.608150 4_1:+15158166 Msa0537210:CDS
AAAGCAAAATTACCATCCCA+TGG 0.612268 4_1:-15160496 None:intergenic
CACGACGGTGAAGGCCGCGA+CGG 0.612289 4_1:+15158140 Msa0537210:CDS
GACATTAGCCTTCGAAGCCG+CGG 0.622925 4_1:-15158088 None:intergenic
TAACACCTTGTCACTCAACA+AGG 0.624473 4_1:+15157984 Msa0537210:CDS
AGCTCTGTTAAATCGTACCT+TGG 0.625217 4_1:+15160394 Msa0537210:CDS
ACTTACCAACGGTTGCTGAA+CGG 0.626940 4_1:-15158326 None:intergenic
CAAAGATGGATTTCCAACAT+GGG 0.627787 4_1:+15158029 Msa0537210:CDS
GAGGAAAATACACATGCACT+TGG 0.629768 4_1:+15157724 Msa0537210:CDS
ACTTCTTCAAACTTCTTCCG+CGG 0.630906 4_1:+15158071 Msa0537210:CDS
AGGGTTTAAATCCGATTGAG+CGG 0.635907 4_1:-15160371 None:intergenic
TAATTCATGGATAGGAAACA+CGG 0.637365 4_1:+15160657 Msa0537210:CDS
ACATACCTTCCACTACCACT+TGG 0.638708 4_1:-15158171 None:intergenic
GGTAGCAAAAGTGTTGGAGG+TGG 0.640198 4_1:+15158291 Msa0537210:CDS
GAGAAGCACTAGTAAGAACA+TGG 0.644219 4_1:-15160597 None:intergenic
TTGGTTAAATAATACTCACA+TGG 0.648130 4_1:-15157616 None:intergenic
TGGATCGGTTCTTCCTTGCG+CGG 0.648642 4_1:-15160308 None:intergenic
AAGGTATGTGATATATGTGA+AGG 0.653633 4_1:+15158185 Msa0537210:CDS
AGGAACCTTGTTGAGTGACA+AGG 0.656613 4_1:-15157989 None:intergenic
TTTCATCTTTGGCAACGCCG+CGG 0.660093 4_1:+15160225 Msa0537210:CDS
GCGACGGATGCTGCACCAAG+TGG 0.664715 4_1:+15158156 Msa0537210:CDS
GGTTGCTGAACGGAAAGTAG+TGG 0.670011 4_1:-15158316 None:intergenic
CCCATGGTAACCAACCAGCA+GGG 0.670836 4_1:-15160480 None:intergenic
ACCGGCAATGAAATTCCCAA+CGG 0.671958 4_1:-15160635 None:intergenic
AAGAAACGCTTAAAGTCCAG+AGG 0.675031 4_1:-15160692 None:intergenic
TTAATTGAGAACTAATCACA+AGG 0.685708 4_1:-15160824 None:intergenic
AATTCATGGATAGGAAACAC+GGG 0.686630 4_1:+15160658 Msa0537210:CDS
ACTTGGTGCAGCATCCGTCG+CGG 0.690402 4_1:-15158154 None:intergenic
AGACATAGTTTGTGACACCA+AGG 0.696122 4_1:-15157924 None:intergenic
AATCGTACCTTGGTAGACCG+TGG 0.701912 4_1:+15160404 Msa0537210:CDS
TGAATTTATCAGAATCACAG+TGG 0.728182 4_1:+15160730 Msa0537210:three_prime_UTR
TGGTTCGGGCAAGTACACGA+CGG 0.758627 4_1:+15158125 Msa0537210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAATATTTAAATTAA+TGG - chr4_1:15158667-15158686 None:intergenic 0.0%
!! AAATATAACAAATAACTTAA+AGG - chr4_1:15158819-15158838 None:intergenic 10.0%
!! AAATAAATAATTATATCAGT+AGG + chr4_1:15158941-15158960 Msa0537210:intron 10.0%
!! AATAAATAATTATATCAGTA+GGG + chr4_1:15158942-15158961 Msa0537210:intron 10.0%
!! TTTCTTAATCATTAAATTAA+GGG - chr4_1:15159287-15159306 None:intergenic 10.0%
!! ATTTCTTAATCATTAAATTA+AGG - chr4_1:15159288-15159307 None:intergenic 10.0%
!! AATTATAATTCATAATTCTA+GGG + chr4_1:15160345-15160364 Msa0537210:CDS 10.0%
!!! TAACAATACAATTAATTTTT+TGG - chr4_1:15160954-15160973 None:intergenic 10.0%
!!! ATTGAAAAATGATGATTTTA+GGG - chr4_1:15157478-15157497 None:intergenic 15.0%
!!! AAAATCGGATTTATTTTTTA+TGG - chr4_1:15157659-15157678 None:intergenic 15.0%
!!! ATTTACAAAAAAAAAAGTGT+TGG + chr4_1:15158777-15158796 Msa0537210:intron 15.0%
!! ATAAATAATTATATCAGTAG+GGG + chr4_1:15158943-15158962 Msa0537210:intron 15.0%
!! GAATAATTATTTATATCCTT+TGG - chr4_1:15159153-15159172 None:intergenic 15.0%
!!! ATTACTTTAAAATCTCTTTA+CGG + chr4_1:15159197-15159216 Msa0537210:intron 15.0%
!! TTAAGATAATATATCATAGA+AGG - chr4_1:15159224-15159243 None:intergenic 15.0%
!! TTCAATTAAATCAATTATTG+TGG - chr4_1:15159319-15159338 None:intergenic 15.0%
!!! ATTCAATTCATTATGTTTTT+GGG - chr4_1:15159355-15159374 None:intergenic 15.0%
!!! TATTCAATTCATTATGTTTT+TGG - chr4_1:15159356-15159375 None:intergenic 15.0%
!! TTAAGGAAAAATATTAATCT+AGG - chr4_1:15159610-15159629 None:intergenic 15.0%
!!! AGATTAATATTTTTCCTTAA+TGG + chr4_1:15159610-15159629 Msa0537210:intron 15.0%
!! ACTTTAACTAATTTCTTAAT+TGG + chr4_1:15159662-15159681 Msa0537210:intron 15.0%
!! TTTAATTAAAAGAAGAATGT+GGG + chr4_1:15159761-15159780 Msa0537210:intron 15.0%
!! GAATTATAATTCATAATTCT+AGG + chr4_1:15160344-15160363 Msa0537210:CDS 15.0%
!! TATGAACAATTTAGTATATA+TGG - chr4_1:15160789-15160808 None:intergenic 15.0%
!! AAAGAAAAAATAATTGAGTA+CGG + chr4_1:15160928-15160947 Msa0537210:three_prime_UTR 15.0%
!! TTATTTAAAAGAATTGTTGT+TGG + chr4_1:15160985-15161004 Msa0537210:three_prime_UTR 15.0%
!!! CATTGAAAAATGATGATTTT+AGG - chr4_1:15157479-15157498 None:intergenic 20.0%
!!! CTAAAATCATCATTTTTCAA+TGG + chr4_1:15157477-15157496 Msa0537210:exon 20.0%
!!! AAGTGAAACTTTGAAAAAAT+CGG - chr4_1:15157674-15157693 None:intergenic 20.0%
!!! TGAATGAACTTTTTTTTTCT+TGG - chr4_1:15158481-15158500 None:intergenic 20.0%
!! CTAGTTTATGATTTAGTTAT+TGG - chr4_1:15158513-15158532 None:intergenic 20.0%
!!! TGCATAAGATTTTTCAATAA+AGG - chr4_1:15158543-15158562 None:intergenic 20.0%
!!! TTTTCTACTCATGAATAAAT+AGG + chr4_1:15158628-15158647 Msa0537210:intron 20.0%
!! GAAAATTACTTATGAATGAT+AGG + chr4_1:15158702-15158721 Msa0537210:intron 20.0%
!!! TACTTATTTTATTTCCATCT+AGG + chr4_1:15158736-15158755 Msa0537210:intron 20.0%
!!! ACTTATTTTATTTCCATCTA+GGG + chr4_1:15158737-15158756 Msa0537210:intron 20.0%
!! AAAATTAATTTAACCCTAGA+TGG - chr4_1:15158753-15158772 None:intergenic 20.0%
!!! AGATTTTAAAGTAATCACAA+TGG - chr4_1:15159192-15159211 None:intergenic 20.0%
!!! ATCATAGTCTAAAACTTTAT+TGG - chr4_1:15159400-15159419 None:intergenic 20.0%
!! TATGATTTGAATCATTTACA+TGG + chr4_1:15159414-15159433 Msa0537210:intron 20.0%
!! GAATAAGACTATATTAAAGT+GGG - chr4_1:15159729-15159748 None:intergenic 20.0%
!! TGAATAAGACTATATTAAAG+TGG - chr4_1:15159730-15159749 None:intergenic 20.0%
!!! CACATTCTTCTTTTAATTAA+AGG - chr4_1:15159762-15159781 None:intergenic 20.0%
!! CTTTAATTAAAAGAAGAATG+TGG + chr4_1:15159760-15159779 Msa0537210:intron 20.0%
!! TTAATTAAAAGAAGAATGTG+GGG + chr4_1:15159762-15159781 Msa0537210:intron 20.0%
!! TTCAACTGATTATTAACTTA+TGG - chr4_1:15159805-15159824 None:intergenic 20.0%
!! AAATTGTTCATAAGATCAAT+TGG + chr4_1:15160797-15160816 Msa0537210:three_prime_UTR 20.0%
!! TAACTAGTAATACATTTGAA+TGG - chr4_1:15160882-15160901 None:intergenic 20.0%
!!! TGTATTACTAGTTAGTTTAT+TGG + chr4_1:15160888-15160907 Msa0537210:three_prime_UTR 20.0%
!!! AAAATGATGATTTTAGGGTA+TGG - chr4_1:15157473-15157492 None:intergenic 25.0%
! ATTAAAAGAGTTGTTACCAA+AGG - chr4_1:15157511-15157530 None:intergenic 25.0%
! CATTAAGTTCCATAAAATCA+TGG + chr4_1:15157569-15157588 Msa0537210:CDS 25.0%
! TTGGTTAAATAATACTCACA+TGG - chr4_1:15157619-15157638 None:intergenic 25.0%
!!! AAAAATGGATTTTATGACCT+TGG + chr4_1:15157907-15157926 Msa0537210:CDS 25.0%
! GTTACATTATTAGACAACAA+AGG - chr4_1:15157952-15157971 None:intergenic 25.0%
!! CTTTAAGGGATAAACTTTAA+GGG - chr4_1:15158393-15158412 None:intergenic 25.0%
!! TCTTTAAGGGATAAACTTTA+AGG - chr4_1:15158394-15158413 None:intergenic 25.0%
!! ATTTGCGAACTTTTCTTTAA+GGG - chr4_1:15158407-15158426 None:intergenic 25.0%
!! TATTTGCGAACTTTTCTTTA+AGG - chr4_1:15158408-15158427 None:intergenic 25.0%
!! CAGGTAAGAACTTTTTAATA+AGG - chr4_1:15158452-15158471 None:intergenic 25.0%
! ACTTATGAATGATAGGTAAA+CGG + chr4_1:15158709-15158728 Msa0537210:intron 25.0%
! ATTATCTACTTTCTTACTCA+AGG - chr4_1:15159127-15159146 None:intergenic 25.0%
!!! CTGCTTGCTTTTATATAATT+AGG - chr4_1:15159248-15159267 None:intergenic 25.0%
! ACATAATGAATTGAATATCG+TGG + chr4_1:15159359-15159378 Msa0537210:intron 25.0%
! TATGTAGCTTTCAAAAATGA+AGG + chr4_1:15159453-15159472 Msa0537210:intron 25.0%
! TTTGTAAAATACCGATAGAT+CGG - chr4_1:15160119-15160138 None:intergenic 25.0%
! TATAGAGAATGTAACATCTA+TGG + chr4_1:15160193-15160212 Msa0537210:CDS 25.0%
! ATAGAGAATGTAACATCTAT+GGG + chr4_1:15160194-15160213 Msa0537210:CDS 25.0%
!!! ATAATTCCAGTGTTTTGATT+TGG - chr4_1:15160331-15160350 None:intergenic 25.0%
! TTAATTGAGAACTAATCACA+AGG - chr4_1:15160827-15160846 None:intergenic 25.0%
ATGATGAGAAACTTAGAGAA+GGG - chr4_1:15157449-15157468 None:intergenic 30.0%
TATGATGAGAAACTTAGAGA+AGG - chr4_1:15157450-15157469 None:intergenic 30.0%
! ACAATAGTGGAGAAAAGAAA+TGG - chr4_1:15157538-15157557 None:intergenic 30.0%
! CTTTTCTCCACTATTGTTTA+TGG + chr4_1:15157541-15157560 Msa0537210:CDS 30.0%
TGAGTAACCATAAACAATAG+TGG - chr4_1:15157551-15157570 None:intergenic 30.0%
! TTGGTTACACCATGATTTTA+TGG - chr4_1:15157581-15157600 None:intergenic 30.0%
AAATAATACTCACATGGTTG+AGG - chr4_1:15157613-15157632 None:intergenic 30.0%
!! GGTTTGATTGAAAGCATTAT+TGG - chr4_1:15157638-15157657 None:intergenic 30.0%
! TGCACTTTGTGTATTTTGTT+TGG - chr4_1:15157831-15157850 None:intergenic 30.0%
AACCTTGAAACATGCAAAAA+TGG + chr4_1:15157892-15157911 Msa0537210:CDS 30.0%
!! ATCCATTTTTGCATGTTTCA+AGG - chr4_1:15157897-15157916 None:intergenic 30.0%
AAGGTATGTGATATATGTGA+AGG + chr4_1:15158185-15158204 Msa0537210:CDS 30.0%
TAAAGCAAAGAATGTCATGT+TGG + chr4_1:15158236-15158255 Msa0537210:CDS 30.0%
ATTGGTAAAACCATAATCAC+TGG + chr4_1:15158270-15158289 Msa0537210:CDS 30.0%
!! TTACTAGAAGTACTTACCAA+CGG - chr4_1:15158340-15158359 None:intergenic 30.0%
AAGGGATAAAAAAAGCTGTA+CGG - chr4_1:15158375-15158394 None:intergenic 30.0%
!!! TTTTTTTTCTTGGTCAAGAC+AGG - chr4_1:15158471-15158490 None:intergenic 30.0%
TGCTTAGAAAGTTAACTAGT+CGG + chr4_1:15158570-15158589 Msa0537210:intron 30.0%
CTTATGAATGATAGGTAAAC+GGG + chr4_1:15158710-15158729 Msa0537210:intron 30.0%
TTATGAATGATAGGTAAACG+GGG + chr4_1:15158711-15158730 Msa0537210:intron 30.0%
TGAGAAGCTATGTATAACAA+CGG + chr4_1:15158987-15159006 Msa0537210:intron 30.0%
TGTTAAGGTTGCATATCTTA+TGG + chr4_1:15159024-15159043 Msa0537210:intron 30.0%
!! GTATTGACTTTAGATTTGTG+TGG + chr4_1:15159063-15159082 Msa0537210:intron 30.0%
CTTTAGATTTGTGTGGTATA+TGG + chr4_1:15159070-15159089 Msa0537210:intron 30.0%
GAAAGTAGATAATAGACCAA+AGG + chr4_1:15159134-15159153 Msa0537210:intron 30.0%
TTTAGCAGTCAAATCTTTAG+AGG + chr4_1:15159526-15159545 Msa0537210:intron 30.0%
GGCTTTCTATATGTTATAGT+CGG - chr4_1:15159560-15159579 None:intergenic 30.0%
! TAGGATGATTTATTCTAGAG+GGG - chr4_1:15159591-15159610 None:intergenic 30.0%
CTAGGATGATTTATTCTAGA+GGG - chr4_1:15159592-15159611 None:intergenic 30.0%
! TCTAGGATGATTTATTCTAG+AGG - chr4_1:15159593-15159612 None:intergenic 30.0%
CACATAGGAAACTATATATG+CGG - chr4_1:15159637-15159656 None:intergenic 30.0%
! TAGTTAAAGTGTTACCACAT+AGG - chr4_1:15159652-15159671 None:intergenic 30.0%
! TTTGGATTGGAATCTACAAT+TGG + chr4_1:15159860-15159879 Msa0537210:intron 30.0%
! TTTTACAAATGTAGCTTCGA+AGG + chr4_1:15160130-15160149 Msa0537210:CDS 30.0%
!! GTTCTTGAATATTTTTGCCA+CGG - chr4_1:15160424-15160443 None:intergenic 30.0%
TTCATGAAAACTGTTCTTGA+TGG + chr4_1:15160448-15160467 Msa0537210:CDS 30.0%
TATGCTGAATATGCTAATAC+AGG + chr4_1:15160532-15160551 Msa0537210:CDS 30.0%
AGGTTCATCAACTTCAAATA+GGG + chr4_1:15160558-15160577 Msa0537210:CDS 30.0%
TAATTCATGGATAGGAAACA+CGG + chr4_1:15160657-15160676 Msa0537210:CDS 30.0%
TGAATTTATCAGAATCACAG+TGG + chr4_1:15160730-15160749 Msa0537210:three_prime_UTR 30.0%
!!! TTTTGGTTTGATGATGAGTG+AGG - chr4_1:15157417-15157436 None:intergenic 35.0%
AACTTAGAGAAGGGTTTATG+AGG - chr4_1:15157440-15157459 None:intergenic 35.0%
TTCAATGGCTTCATTTCCTT+TGG + chr4_1:15157492-15157511 Msa0537210:exon 35.0%
AGTTTCACTTGAACTTGCAA+TGG + chr4_1:15157684-15157703 Msa0537210:CDS 35.0%
TGCAATGGAAAGAGCAAAAA+AGG + chr4_1:15157699-15157718 Msa0537210:CDS 35.0%
GCAATGGAAAGAGCAAAAAA+GGG + chr4_1:15157700-15157719 Msa0537210:CDS 35.0%
CACAATATGAATCCCAAACA+TGG + chr4_1:15157851-15157870 Msa0537210:CDS 35.0%
TTGTAACTTGGTTGTTGGTA+AGG - chr4_1:15158012-15158031 None:intergenic 35.0%
CATCTTTGTAACTTGGTTGT+TGG - chr4_1:15158017-15158036 None:intergenic 35.0%
CAACAACCAAGTTACAAAGA+TGG + chr4_1:15158015-15158034 Msa0537210:CDS 35.0%
GGAAATCCATCTTTGTAACT+TGG - chr4_1:15158024-15158043 None:intergenic 35.0%
! ACAAAGATGGATTTCCAACA+TGG + chr4_1:15158028-15158047 Msa0537210:CDS 35.0%
! CAAAGATGGATTTCCAACAT+GGG + chr4_1:15158029-15158048 Msa0537210:CDS 35.0%
TATGTGATATATGTGAAGGC+TGG + chr4_1:15158189-15158208 Msa0537210:CDS 35.0%
! ACTTTTGCTACCAGTGATTA+TGG - chr4_1:15158283-15158302 None:intergenic 35.0%
TACATAGCTTCTCATTCTAG+AGG - chr4_1:15158981-15159000 None:intergenic 35.0%
AAGATATGCAACCTTAACAC+CGG - chr4_1:15159023-15159042 None:intergenic 35.0%
!!! TGTTTTTGGGCTCAATTATG+TGG - chr4_1:15159342-15159361 None:intergenic 35.0%
CACATGTATTGTCTTACGTT+TGG + chr4_1:15159478-15159497 Msa0537210:intron 35.0%
ACTATATATGCGGTCCATTA+AGG - chr4_1:15159627-15159646 None:intergenic 35.0%
CGCATATATAGTTTCCTATG+TGG + chr4_1:15159635-15159654 Msa0537210:intron 35.0%
TATTAAAGTGGGTCCTACAT+TGG - chr4_1:15159718-15159737 None:intergenic 35.0%
GAGTATTAAAAAACCTAGCG+TGG - chr4_1:15159983-15160002 None:intergenic 35.0%
!!! TTTTTTTTTGCAGCTGCTAC+TGG + chr4_1:15160010-15160029 Msa0537210:intron 35.0%
! ACAATTTTGCAAAACTACCG+CGG - chr4_1:15160245-15160264 None:intergenic 35.0%
!!! TCCAGTGTTTTGATTTGGAT+CGG - chr4_1:15160326-15160345 None:intergenic 35.0%
AAAGCAAAATTACCATCCCA+TGG - chr4_1:15160499-15160518 None:intergenic 35.0%
! ATGGGATGGTAATTTTGCTT+TGG + chr4_1:15160498-15160517 Msa0537210:CDS 35.0%
GAATATGCTAATACAGGTTC+AGG + chr4_1:15160538-15160557 Msa0537210:CDS 35.0%
CAGGTTCATCAACTTCAAAT+AGG + chr4_1:15160557-15160576 Msa0537210:CDS 35.0%
CAACTTCAAATAGGGTTACA+TGG + chr4_1:15160566-15160585 Msa0537210:CDS 35.0%
TCAAATAGGGTTACATGGAA+AGG + chr4_1:15160571-15160590 Msa0537210:CDS 35.0%
ATTTCATTGCCGGTAATTCA+TGG + chr4_1:15160644-15160663 Msa0537210:CDS 35.0%
GTTTCCTATCCATGAATTAC+CGG - chr4_1:15160656-15160675 None:intergenic 35.0%
AATTCATGGATAGGAAACAC+GGG + chr4_1:15160658-15160677 Msa0537210:CDS 35.0%
! TATTGGCTGTTCTACTGTAT+AGG + chr4_1:15160905-15160924 Msa0537210:three_prime_UTR 35.0%
! GAGAAGGGTTTATGAGGTTT+TGG - chr4_1:15157434-15157453 None:intergenic 40.0%
ACTCACATGGTTGAGGATAA+GGG - chr4_1:15157606-15157625 None:intergenic 40.0%
TACTCACATGGTTGAGGATA+AGG - chr4_1:15157607-15157626 None:intergenic 40.0%
GAGGAAAATACACATGCACT+TGG + chr4_1:15157724-15157743 Msa0537210:CDS 40.0%
AGCTGTACTAAGCCATGTTT+GGG - chr4_1:15157866-15157885 None:intergenic 40.0%
AAGCTGTACTAAGCCATGTT+TGG - chr4_1:15157867-15157886 None:intergenic 40.0%
! AGACATAGTTTGTGACACCA+AGG - chr4_1:15157927-15157946 None:intergenic 40.0%
TAACACCTTGTCACTCAACA+AGG + chr4_1:15157984-15158003 Msa0537210:CDS 40.0%
! TTTCCAACATGGGTTAAACC+TGG + chr4_1:15158039-15158058 Msa0537210:CDS 40.0%
TGAAGAAGTTTCCTATCACC+AGG - chr4_1:15158060-15158079 None:intergenic 40.0%
ACTTCTTCAAACTTCTTCCG+CGG + chr4_1:15158071-15158090 Msa0537210:CDS 40.0%
GCAAAGAATGTCATGTTGGT+TGG + chr4_1:15158240-15158259 Msa0537210:CDS 40.0%
! AATGTCATGTTGGTTGGAGA+TGG + chr4_1:15158246-15158265 Msa0537210:CDS 40.0%
! ATGTTGGTTGGAGATGGTAT+TGG + chr4_1:15158252-15158271 Msa0537210:CDS 40.0%
! ATCACTGGTAGCAAAAGTGT+TGG + chr4_1:15158285-15158304 Msa0537210:CDS 40.0%
TGTATAACAACGGTCCTTCT+CGG + chr4_1:15158997-15159016 Msa0537210:intron 40.0%
GTATTGTCTTACGTTTGGCT+AGG + chr4_1:15159483-15159502 Msa0537210:intron 40.0%
! TATTCTAGAGGGGAAAGATG+TGG - chr4_1:15159581-15159600 None:intergenic 40.0%
CACAATTCCACATCCAATGT+AGG + chr4_1:15159702-15159721 Msa0537210:intron 40.0%
! AGTACACACGTCATGCATTT+TGG + chr4_1:15159842-15159861 Msa0537210:intron 40.0%
! ACACGTCATGCATTTTGGAT+TGG + chr4_1:15159847-15159866 Msa0537210:intron 40.0%
ATCACATTCAGAAACACTGC+TGG + chr4_1:15160055-15160074 Msa0537210:CDS 40.0%
! GGGACAGTTGATTTCATCTT+TGG + chr4_1:15160214-15160233 Msa0537210:CDS 40.0%
! GTAACTGTGATGGTTTTTGC+AGG - chr4_1:15160289-15160308 None:intergenic 40.0%
ACCGATCCAAATCAAAACAC+TGG + chr4_1:15160322-15160341 Msa0537210:CDS 40.0%
AGGGTTTAAATCCGATTGAG+CGG - chr4_1:15160374-15160393 None:intergenic 40.0%
GGTACGATTTAACAGAGCTA+GGG - chr4_1:15160393-15160412 None:intergenic 40.0%
AGGTACGATTTAACAGAGCT+AGG - chr4_1:15160394-15160413 None:intergenic 40.0%
AGCTCTGTTAAATCGTACCT+TGG + chr4_1:15160394-15160413 Msa0537210:CDS 40.0%
!!! GATGGTTTTATTAACCCTGC+TGG + chr4_1:15160466-15160485 Msa0537210:CDS 40.0%
!! GTTTTATTAACCCTGCTGGT+TGG + chr4_1:15160470-15160489 Msa0537210:CDS 40.0%
!! GAGAAGCACTAGTAAGAACA+TGG - chr4_1:15160600-15160619 None:intergenic 40.0%
ATGAAATTCCCAACGGTGAA+AGG - chr4_1:15160631-15160650 None:intergenic 40.0%
ATTGCCGGTAATTCATGGAT+AGG + chr4_1:15160649-15160668 Msa0537210:CDS 40.0%
AAGAAACGCTTAAAGTCCAG+AGG - chr4_1:15160695-15160714 None:intergenic 40.0%
CTGGACTTTAAGCGTTTCTT+TGG + chr4_1:15160695-15160714 Msa0537210:exon 40.0%
ATGGTTGAGGATAAGGGGTT+TGG - chr4_1:15157600-15157619 None:intergenic 45.0%
CTCACATGGTTGAGGATAAG+GGG - chr4_1:15157605-15157624 None:intergenic 45.0%
GGAAAGAGCAAAAAAGGGTG+AGG + chr4_1:15157705-15157724 Msa0537210:CDS 45.0%
!! CAGCTTTTTCTTGTGGGCTA+CGG - chr4_1:15157759-15157778 None:intergenic 45.0%
!! CCCAAGCAGCTTTTTCTTGT+GGG - chr4_1:15157765-15157784 None:intergenic 45.0%
CCCACAAGAAAAAGCTGCTT+GGG + chr4_1:15157762-15157781 Msa0537210:CDS 45.0%
AGGAACCTTGTTGAGTGACA+AGG - chr4_1:15157992-15158011 None:intergenic 45.0%
TCACCAGGTTTAACCCATGT+TGG - chr4_1:15158045-15158064 None:intergenic 45.0%
! CATGGGTTAAACCTGGTGAT+AGG + chr4_1:15158046-15158065 Msa0537210:CDS 45.0%
AATGTCGTCGTTGCTAAGGA+TGG + chr4_1:15158105-15158124 Msa0537210:CDS 45.0%
ACATACCTTCCACTACCACT+TGG - chr4_1:15158174-15158193 None:intergenic 45.0%
!! ACTGGTAGCAAAAGTGTTGG+AGG + chr4_1:15158288-15158307 Msa0537210:CDS 45.0%
ACTTACCAACGGTTGCTGAA+CGG - chr4_1:15158329-15158348 None:intergenic 45.0%
TCTTACGTTTGGCTAGGACA+AGG + chr4_1:15159489-15159508 Msa0537210:intron 45.0%
CAAGCTGTTGCATTTCGTTC+CGG + chr4_1:15160091-15160110 Msa0537210:CDS 45.0%
AAATACCGATAGATCGGAGC+CGG - chr4_1:15160113-15160132 None:intergenic 45.0%
! ACTGTGATGGTTTTTGCAGG+CGG - chr4_1:15160286-15160305 None:intergenic 45.0%
!! TTCTAGGGTTTCCGCTCAAT+CGG + chr4_1:15160360-15160379 Msa0537210:CDS 45.0%
! ATTTTTGCCACGGTCTACCA+AGG - chr4_1:15160414-15160433 None:intergenic 45.0%
AAGCTTCACCTTTCACCGTT+GGG + chr4_1:15160620-15160639 Msa0537210:CDS 45.0%
ACCGTTGGGAATTTCATTGC+CGG + chr4_1:15160634-15160653 Msa0537210:CDS 45.0%
ACCGGCAATGAAATTCCCAA+CGG - chr4_1:15160638-15160657 None:intergenic 45.0%
GCTTAAAGTCCAGAGGTGAA+TGG - chr4_1:15160688-15160707 None:intergenic 45.0%
GCCCACAAGAAAAAGCTGCT+TGG + chr4_1:15157761-15157780 Msa0537210:CDS 50.0%
!! GCCCAAGCAGCTTTTTCTTG+TGG - chr4_1:15157766-15157785 None:intergenic 50.0%
GGCTAATGTCGTCGTTGCTA+AGG + chr4_1:15158101-15158120 Msa0537210:CDS 50.0%
CGTCGTTGCTAAGGATGGTT+CGG + chr4_1:15158110-15158129 Msa0537210:CDS 50.0%
GTCGTTGCTAAGGATGGTTC+GGG + chr4_1:15158111-15158130 Msa0537210:CDS 50.0%
!! GGTAGCAAAAGTGTTGGAGG+TGG + chr4_1:15158291-15158310 Msa0537210:CDS 50.0%
GGTTGCTGAACGGAAAGTAG+TGG - chr4_1:15158319-15158338 None:intergenic 50.0%
ACTTTCCGTTCAGCAACCGT+TGG + chr4_1:15158321-15158340 Msa0537210:CDS 50.0%
AGTGGGTCCTACATTGGATG+TGG - chr4_1:15159712-15159731 None:intergenic 50.0%
AATTGCTTGTGCTCCACGCT+AGG + chr4_1:15159967-15159986 Msa0537210:intron 50.0%
TTTGCAGCTGCTACTGGTGA+TGG + chr4_1:15160016-15160035 Msa0537210:intron 50.0%
TTTCATCTTTGGCAACGCCG+CGG + chr4_1:15160225-15160244 Msa0537210:CDS 50.0%
! CTGTGATGGTTTTTGCAGGC+GGG - chr4_1:15160285-15160304 None:intergenic 50.0%
AATCGTACCTTGGTAGACCG+TGG + chr4_1:15160404-15160423 Msa0537210:CDS 50.0%
CAAGCTTCACCTTTCACCGT+TGG + chr4_1:15160619-15160638 Msa0537210:CDS 50.0%
! TCTTGTGGGCTACGGCACTT+TGG - chr4_1:15157751-15157770 None:intergenic 55.0%
AACTTCTTCCGCGGCTTCGA+AGG + chr4_1:15158080-15158099 Msa0537210:CDS 55.0%
!! GACATTAGCCTTCGAAGCCG+CGG - chr4_1:15158091-15158110 None:intergenic 55.0%
! TGGTTCGGGCAAGTACACGA+CGG + chr4_1:15158125-15158144 Msa0537210:CDS 55.0%
GATGCTGCACCAAGTGGTAG+TGG + chr4_1:15158162-15158181 Msa0537210:CDS 55.0%
!! CTGCACCAAGTGGTAGTGGA+AGG + chr4_1:15158166-15158185 Msa0537210:CDS 55.0%
TAACAACGGTCCTTCTCGGC+CGG + chr4_1:15159001-15159020 Msa0537210:intron 55.0%
AACCTTAACACCGGCCGAGA+AGG - chr4_1:15159014-15159033 None:intergenic 55.0%
TCGTTCCGGCTCCGATCTAT+CGG + chr4_1:15160105-15160124 Msa0537210:CDS 55.0%
ACCATCACAGTTACCGCGCA+AGG + chr4_1:15160295-15160314 Msa0537210:CDS 55.0%
TCCTTGCGCGGTAACTGTGA+TGG - chr4_1:15160299-15160318 None:intergenic 55.0%
! TGGATCGGTTCTTCCTTGCG+CGG - chr4_1:15160311-15160330 None:intergenic 55.0%
! ACCCTGCTGGTTGGTTACCA+TGG + chr4_1:15160479-15160498 Msa0537210:CDS 55.0%
CCCATGGTAACCAACCAGCA+GGG - chr4_1:15160483-15160502 None:intergenic 55.0%
! CCCTGCTGGTTGGTTACCAT+GGG + chr4_1:15160480-15160499 Msa0537210:CDS 55.0%
TCCCATGGTAACCAACCAGC+AGG - chr4_1:15160484-15160503 None:intergenic 55.0%
! GCTGGTTGGTTACCATGGGA+TGG + chr4_1:15160484-15160503 Msa0537210:CDS 55.0%
GGGCAAGTACACGACGGTGA+AGG + chr4_1:15158131-15158150 Msa0537210:CDS 60.0%
!! ACTTGGTGCAGCATCCGTCG+CGG - chr4_1:15158157-15158176 None:intergenic 60.0%
! GTCCTTCTCGGCCGGTGTTA+AGG + chr4_1:15159009-15159028 Msa0537210:intron 60.0%
! ACGGGTGTGCCATTCACCTC+TGG + chr4_1:15160676-15160695 Msa0537210:CDS 60.0%
GCGACGGATGCTGCACCAAG+TGG + chr4_1:15158156-15158175 Msa0537210:CDS 65.0%
CACGACGGTGAAGGCCGCGA+CGG + chr4_1:15158140-15158159 Msa0537210:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 15157395 15161057 15157395 ID=Msa0537210;Name=Msa0537210
chr4_1 mRNA 15157395 15161057 15157395 ID=Msa0537210-mRNA-1;Parent=Msa0537210;Name=Msa0537210-mRNA-1;_AED=0.00;_eAED=0.00;_QI=101|1|1|1|1|1|2|352|509
chr4_1 exon 15157395 15158342 15157395 ID=Msa0537210-mRNA-1:exon:4709;Parent=Msa0537210-mRNA-1
chr4_1 exon 15160023 15161057 15160023 ID=Msa0537210-mRNA-1:exon:4710;Parent=Msa0537210-mRNA-1
chr4_1 five_prime_UTR 15157395 15157495 15157395 ID=Msa0537210-mRNA-1:five_prime_utr;Parent=Msa0537210-mRNA-1
chr4_1 CDS 15157496 15158342 15157496 ID=Msa0537210-mRNA-1:cds;Parent=Msa0537210-mRNA-1
chr4_1 CDS 15160023 15160705 15160023 ID=Msa0537210-mRNA-1:cds;Parent=Msa0537210-mRNA-1
chr4_1 three_prime_UTR 15160706 15161057 15160706 ID=Msa0537210-mRNA-1:three_prime_utr;Parent=Msa0537210-mRNA-1
Gene Sequence

>Msa0537210

ATGGCTTCATTTCCTTTGGTAACAACTCTTTTAATATTTCCATTTCTTTTCTCCACTATTGTTTATGGTTACTCATTAAGTTCCATAAAATCATGGTGTAACCAAACCCCTTATCCTCAACCATGTGAGTATTATTTAACCAATAATGCTTTCAATCAAACCATAAAAAATAAATCCGATTTTTTCAAAGTTTCACTTGAACTTGCAATGGAAAGAGCAAAAAAGGGTGAGGAAAATACACATGCACTTGGTCCAAAGTGCCGTAGCCCACAAGAAAAAGCTGCTTGGGCAGATTGTCTTGAACTCTATGATTTCACTGTTCAAAAGCTTAGCCAAACAAAATACACAAAGTGCACACAATATGAATCCCAAACATGGCTTAGTACAGCTTTAACAAACCTTGAAACATGCAAAAATGGATTTTATGACCTTGGTGTCACAAACTATGTCTTACCTTTGTTGTCTAATAATGTAACAAAGTTGTTAAGTAACACCTTGTCACTCAACAAGGTTCCTTACCAACAACCAAGTTACAAAGATGGATTTCCAACATGGGTTAAACCTGGTGATAGGAAACTTCTTCAAACTTCTTCCGCGGCTTCGAAGGCTAATGTCGTCGTTGCTAAGGATGGTTCGGGCAAGTACACGACGGTGAAGGCCGCGACGGATGCTGCACCAAGTGGTAGTGGAAGGTATGTGATATATGTGAAGGCTGGTGTGTATAATGAACAAGTTGAGATTAAAGCAAAGAATGTCATGTTGGTTGGAGATGGTATTGGTAAAACCATAATCACTGGTAGCAAAAGTGTTGGAGGTGGCACCACTACTTTCCGTTCAGCAACCGTTGCTGCTACTGGTGATGGATTTATTGCTCAAGACATCACATTCAGAAACACTGCTGGAGCTGCAAATCATCAAGCTGTTGCATTTCGTTCCGGCTCCGATCTATCGGTATTTTACAAATGTAGCTTCGAAGGTTATCAAGACACACTTTATGTTCACTCAGAGAGACAATTTTATAGAGAATGTAACATCTATGGGACAGTTGATTTCATCTTTGGCAACGCCGCGGTAGTTTTGCAAAATTGTAACATTTTTGCAAGAAACCCGCCTGCAAAAACCATCACAGTTACCGCGCAAGGAAGAACCGATCCAAATCAAAACACTGGAATTATAATTCATAATTCTAGGGTTTCCGCTCAATCGGATTTAAACCCTAGCTCTGTTAAATCGTACCTTGGTAGACCGTGGCAAAAATATTCAAGAACAGTTTTCATGAAAACTGTTCTTGATGGTTTTATTAACCCTGCTGGTTGGTTACCATGGGATGGTAATTTTGCTTTGGATACTTTATATTATGCTGAATATGCTAATACAGGTTCAGGTTCATCAACTTCAAATAGGGTTACATGGAAAGGTTACCATGTTCTTACTAGTGCTTCTCAAGCTTCACCTTTCACCGTTGGGAATTTCATTGCCGGTAATTCATGGATAGGAAACACGGGTGTGCCATTCACCTCTGGACTTTAA
Protein sequence

>Msa0537210

MASFPLVTTLLIFPFLFSTIVYGYSLSSIKSWCNQTPYPQPCEYYLTNNAFNQTIKNKSDFFKVSLELAMERAKKGEENTHALGPKCRSPQEKAAWADCLELYDFTVQKLSQTKYTKCTQYESQTWLSTALTNLETCKNGFYDLGVTNYVLPLLSNNVTKLLSNTLSLNKVPYQQPSYKDGFPTWVKPGDRKLLQTSSAASKANVVVAKDGSGKYTTVKAATDAAPSGSGRYVIYVKAGVYNEQVEIKAKNVMLVGDGIGKTIITGSKSVGGGTTTFRSATVAATGDGFIAQDITFRNTAGAANHQAVAFRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRECNIYGTVDFIFGNAAVVLQNCNIFARNPPAKTITVTAQGRTDPNQNTGIIIHNSRVSAQSDLNPSSVKSYLGRPWQKYSRTVFMKTVLDGFINPAGWLPWDGNFALDTLYYAEYANTGSGSSTSNRVTWKGYHVLTSASQASPFTVGNFIAGNSWIGNTGVPFTSGL*