AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0672090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0672090 MtrunA17_Chr4g0011791 98.824 510 5 1 1 510 1 509 0.0 1040
Msa0672090 MtrunA17_Chr4g0011771 72.921 517 129 6 1 510 1 513 0.0 772
Msa0672090 MtrunA17_Chr8g0391311 48.885 538 234 13 4 510 14 541 1.09e-175 505
Msa0672090 MtrunA17_Chr8g0391291 48.885 538 234 13 4 510 12 539 1.51e-175 504
Msa0672090 MtrunA17_Chr4g0018371 50.099 505 227 10 30 510 36 539 1.73e-169 489
Msa0672090 MtrunA17_Chr3g0090091 49.901 507 230 11 24 510 26 528 3.77e-166 480
Msa0672090 MtrunA17_Chr3g0079341 49.209 506 234 12 24 510 26 527 5.69e-163 472
Msa0672090 MtrunA17_Chr3g0079361 48.952 525 240 14 10 510 7 527 6.34e-163 472
Msa0672090 MtrunA17_Chr3g0079351 49.407 506 233 12 24 510 26 527 7.99e-162 469
Msa0672090 MtrunA17_Chr7g0269541 46.886 546 249 13 1 506 8 552 2.36e-160 467
Msa0672090 MtrunA17_Chr7g0233771 46.589 513 243 11 25 510 46 554 2.27e-147 433
Msa0672090 MtrunA17_Chr8g0345281 46.869 527 229 14 30 510 68 589 3.26e-146 432
Msa0672090 MtrunA17_Chr4g0011751 51.740 431 186 9 96 510 65 489 7.58e-144 422
Msa0672090 MtrunA17_Chr7g0272141 49.443 449 189 10 92 510 102 542 4.11e-142 419
Msa0672090 MtrunA17_Chr7g0233831 50.334 449 192 11 87 510 113 555 7.97e-142 419
Msa0672090 MtrunA17_Chr4g0069841 51.025 439 183 9 96 510 86 516 3.91e-141 416
Msa0672090 MtrunA17_Chr3g0126771 43.511 524 251 11 29 510 77 597 1.39e-140 417
Msa0672090 MtrunA17_Chr1g0192331 45.880 534 243 14 7 510 11 528 2.04e-140 415
Msa0672090 MtrunA17_Chr2g0302021 44.297 526 253 10 8 510 17 525 2.55e-139 412
Msa0672090 MtrunA17_Chr1g0195331 46.429 532 222 16 34 510 32 555 7.40e-139 412
Msa0672090 MtrunA17_Chr1g0195301 46.241 532 223 16 34 510 32 555 8.42e-139 412
Msa0672090 MtrunA17_Chr1g0195321 46.241 532 223 16 34 510 32 555 8.42e-139 412
Msa0672090 MtrunA17_Chr1g0147051 42.301 539 262 12 19 510 71 607 1.39e-137 410
Msa0672090 MtrunA17_Chr1g0175661 50.108 465 180 14 96 510 96 558 2.95e-137 408
Msa0672090 MtrunA17_Chr7g0233801 44.574 516 248 14 25 510 46 553 9.48e-137 406
Msa0672090 MtrunA17_Chr1g0195351 45.865 532 223 17 34 510 32 553 1.03e-134 401
Msa0672090 MtrunA17_Chr7g0269531 44.906 530 232 15 34 510 43 565 1.63e-134 401
Msa0672090 MtrunA17_Chr3g0080391 44.660 515 244 12 28 510 69 574 1.05e-133 399
Msa0672090 MtrunA17_Chr8g0354901 45.703 512 245 15 28 510 74 581 3.10e-131 393
Msa0672090 MtrunA17_Chr7g0269511 44.925 532 229 16 34 510 42 564 3.73e-131 392
Msa0672090 MtrunA17_Chr7g0269521 43.985 532 240 14 31 510 37 562 1.85e-128 385
Msa0672090 MtrunA17_Chr8g0354911 43.327 547 228 15 34 510 78 612 3.90e-128 386
Msa0672090 MtrunA17_Chr4g0069881 43.701 508 253 14 27 510 37 535 1.87e-127 382
Msa0672090 MtrunA17_Chr4g0069861 45.475 442 207 8 96 510 83 517 4.36e-125 375
Msa0672090 MtrunA17_Chr4g0069891 41.143 525 265 13 24 510 75 593 1.48e-123 374
Msa0672090 MtrunA17_Chr1g0195311 64.639 263 89 3 251 510 53 314 2.20e-120 356
Msa0672090 MtrunA17_Chr3g0080381 41.989 543 243 17 34 510 92 628 6.85e-118 360
Msa0672090 MtrunA17_Chr1g0194091 40.812 468 228 10 91 510 103 569 3.84e-112 343
Msa0672090 MtrunA17_Chr1g0192321 38.390 534 276 14 26 509 52 582 4.90e-111 341
Msa0672090 MtrunA17_Chr8g0345211 39.258 512 278 11 28 510 75 582 2.34e-107 332
Msa0672090 MtrunA17_Chr1g0192311 35.674 527 279 13 26 506 54 566 1.57e-104 324
Msa0672090 MtrunA17_Chr1g0192281 35.674 527 279 13 26 506 54 566 1.57e-104 324
Msa0672090 MtrunA17_Chr7g0272151 37.406 532 281 16 26 509 55 582 1.36e-103 322
Msa0672090 MtrunA17_Chr3g0129921 37.091 550 291 17 8 509 21 563 9.39e-102 317
Msa0672090 MtrunA17_Chr1g0194081 38.618 492 259 13 60 510 74 563 1.02e-101 317
Msa0672090 MtrunA17_Chr7g0270691 36.775 552 297 17 1 510 8 549 5.60e-101 314
Msa0672090 MtrunA17_Chr8g0384121 38.477 512 263 14 28 499 54 553 1.47e-100 314
Msa0672090 MtrunA17_Chr7g0270661 39.485 466 226 10 94 505 106 569 2.13e-99 311
Msa0672090 MtrunA17_Chr7g0270671 39.485 466 226 10 94 505 106 569 2.13e-99 311
Msa0672090 MtrunA17_Chr1g0192301 34.690 516 299 12 26 506 59 571 1.15e-98 309
Msa0672090 MtrunA17_Chr4g0000121 34.345 527 286 13 26 506 41 553 5.78e-98 307
Msa0672090 MtrunA17_Chr7g0270651 37.792 471 237 11 94 510 106 574 9.90e-97 304
Msa0672090 MtrunA17_Chr7g0234711 61.842 228 83 3 287 510 42 269 6.24e-96 291
Msa0672090 MtrunA17_Chr2g0302001 47.368 323 158 6 192 510 123 437 2.06e-95 296
Msa0672090 MtrunA17_Chr4g0067611 34.924 524 294 14 26 509 55 571 1.87e-92 293
Msa0672090 MtrunA17_Chr2g0301931 49.291 282 137 5 232 510 14 292 3.71e-91 280
Msa0672090 MtrunA17_Chr2g0295551 35.135 518 296 14 26 509 52 563 1.02e-90 288
Msa0672090 MtrunA17_Chr4g0067601 34.549 521 292 13 30 509 59 571 4.36e-90 287
Msa0672090 MtrunA17_Chr7g0270631 37.838 444 229 8 94 490 94 537 8.15e-89 295
Msa0672090 MtrunA17_Chr7g0270631 36.484 455 224 11 94 488 642 1091 5.31e-77 262
Msa0672090 MtrunA17_Chr2g0295531 33.399 506 306 12 29 507 42 543 2.64e-88 283
Msa0672090 MtrunA17_Chr2g0295541 33.333 549 295 15 26 509 52 594 4.15e-85 274
Msa0672090 MtrunA17_Chr8g0380581 43.917 337 169 10 183 507 45 373 1.37e-83 263
Msa0672090 MtrunA17_Chr5g0437831 40.762 341 189 8 180 510 46 383 1.15e-77 248
Msa0672090 MtrunA17_Chr7g0268241 40.557 323 176 7 194 510 38 350 1.04e-71 232
Msa0672090 MtrunA17_Chr2g0334111 40.705 312 167 7 205 510 24 323 6.15e-71 229
Msa0672090 MtrunA17_Chr7g0271381 30.214 513 316 16 26 509 56 555 6.94e-68 228
Msa0672090 MtrunA17_Chr8g0391261 63.235 136 48 1 377 510 31 166 1.44e-56 186
Msa0672090 MtrunA17_Chr7g0234731 39.781 274 146 9 24 282 45 314 1.45e-53 183
Msa0672090 MtrunA17_Chr6g0487101 39.735 302 152 9 214 499 93 380 3.98e-51 179
Msa0672090 MtrunA17_Chr8g0391281 35.179 307 161 11 4 282 12 308 5.85e-50 174
Msa0672090 MtrunA17_Chr6g0453551 35.644 303 167 9 206 493 6 295 2.40e-49 172
Msa0672090 MtrunA17_Chr8g0389771 37.419 310 164 9 207 499 81 377 2.58e-49 174
Msa0672090 MtrunA17_Chr6g0466951 34.296 277 159 7 204 472 67 328 3.17e-45 162
Msa0672090 MtrunA17_Chr7g0217941 34.448 299 172 7 208 493 8 295 5.43e-45 160
Msa0672090 MtrunA17_Chr2g0299431 42.918 233 126 5 180 407 28 258 1.18e-44 159
Msa0672090 MtrunA17_Chr1g0194311 32.919 322 189 8 191 499 61 368 5.07e-44 159
Msa0672090 MtrunA17_Chr7g0258341 32.132 333 194 9 181 499 73 387 1.54e-43 158
Msa0672090 MtrunA17_Chr6g0466961 34.545 275 157 6 206 472 40 299 6.16e-42 152
Msa0672090 MtrunA17_Chr2g0294321 31.746 315 192 8 195 499 45 346 1.59e-40 149
Msa0672090 MtrunA17_Chr1g0161731 30.892 314 186 8 201 503 44 337 8.45e-39 144
Msa0672090 MtrunA17_Chr5g0446301 33.225 307 170 8 212 499 67 357 1.85e-37 141
Msa0672090 MtrunA17_Chr4g0070051 31.889 323 185 11 194 499 39 343 7.03e-36 136
Msa0672090 MtrunA17_Chr2g0295851 31.803 305 174 11 206 499 55 336 3.06e-34 132
Msa0672090 MtrunA17_Chr5g0399221 29.808 312 192 8 206 502 63 362 5.36e-33 129
Msa0672090 MtrunA17_Chr7g0265201 30.492 305 183 7 208 501 101 387 1.70e-31 125
Msa0672090 MtrunA17_Chr5g0440871 29.195 298 193 6 206 501 39 320 2.93e-31 123
Msa0672090 MtrunA17_Chr4g0037751 29.063 320 193 11 195 499 61 361 3.14e-31 124
Msa0672090 MtrunA17_Chr5g0437821 43.182 132 71 2 316 443 4 135 3.86e-31 117
Msa0672090 MtrunA17_Chr8g0351871 27.541 305 195 6 206 499 33 322 3.97e-31 123
Msa0672090 MtrunA17_Chr6g0459781 37.853 177 99 4 199 364 6 182 1.79e-30 117
Msa0672090 MtrunA17_Chr5g0420831 28.280 343 218 10 172 499 32 361 2.02e-29 119
Msa0672090 MtrunA17_Chr4g0025841 31.276 243 148 6 206 444 37 264 1.84e-24 102
Msa0672090 MtrunA17_Chr5g0440901 35.099 151 89 3 295 444 1 143 6.49e-23 94.7
Msa0672090 MtrunA17_Chr2g0314691 26.415 212 140 4 291 499 3 201 2.39e-21 92.4
Msa0672090 MtrunA17_Chr7g0265161 31.461 178 92 4 268 440 5 157 4.61e-17 80.9
Msa0672090 MtrunA17_Chr6g0460141 36.567 134 75 3 233 356 5 138 5.19e-16 75.5
Msa0672090 MtrunA17_Chr5g0440891 32.692 156 93 5 206 357 48 195 1.64e-14 72.4
Msa0672090 MtrunA17_Chr6g0459791 37.398 123 66 4 378 499 4 116 5.23e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0672090 AT2G45220.1 66.796 515 162 6 1 510 1 511 0.0 713
Msa0672090 AT5G51490.1 52.183 504 211 10 33 510 37 536 2.75e-176 507
Msa0672090 AT5G51500.1 50.690 507 224 9 28 510 36 540 1.43e-167 485
Msa0672090 AT3G47400.1 49.016 508 229 10 33 510 87 594 3.13e-162 473
Msa0672090 AT4G00190.1 49.691 485 218 8 34 510 8 474 1.05e-161 468
Msa0672090 AT1G23200.1 46.140 544 231 13 20 510 20 554 6.33e-158 461
Msa0672090 AT3G49220.1 44.727 512 252 9 29 510 86 596 4.31e-142 422
Msa0672090 AT3G49220.3 44.727 512 252 9 29 510 131 641 9.80e-142 422
Msa0672090 AT5G53370.1 44.444 513 253 8 29 510 74 585 1.04e-141 421
Msa0672090 AT5G53370.2 44.444 513 253 8 29 510 110 621 1.13e-141 422
Msa0672090 AT3G59010.1 51.157 432 184 5 99 510 104 528 1.93e-141 418
Msa0672090 AT1G11580.1 48.523 474 223 9 58 510 84 557 3.04e-141 418
Msa0672090 AT3G14310.1 45.102 541 238 13 26 510 55 592 4.31e-141 419
Msa0672090 AT1G11580.2 48.523 474 223 9 58 510 105 578 8.00e-141 418
Msa0672090 AT1G53830.1 45.185 540 243 13 20 510 52 587 2.52e-139 414
Msa0672090 AT3G05620.1 46.091 486 219 11 58 510 66 541 3.92e-136 405
Msa0672090 AT3G43270.1 48.107 449 196 9 96 510 80 525 1.07e-135 403
Msa0672090 AT4G02320.1 47.661 449 206 10 90 510 71 518 3.58e-135 401
Msa0672090 AT3G60730.1 45.833 456 206 8 94 510 66 519 1.52e-132 395
Msa0672090 AT2G43050.1 49.296 426 193 9 99 510 100 516 1.44e-130 389
Msa0672090 AT4G33220.1 45.887 462 197 9 96 510 68 523 1.38e-129 387
Msa0672090 AT4G02330.1 41.667 576 252 19 7 510 8 571 1.92e-127 383
Msa0672090 AT2G47550.1 49.676 463 184 13 95 510 98 558 2.80e-127 382
Msa0672090 AT4G02300.1 45.598 443 216 8 93 510 90 532 3.18e-127 381
Msa0672090 AT1G02810.1 41.186 590 246 18 6 510 4 577 5.98e-126 380
Msa0672090 AT2G26450.1 42.913 508 258 15 30 510 109 611 1.30e-123 375
Msa0672090 AT4G33220.2 54.491 334 144 5 183 510 71 402 6.56e-123 366
Msa0672090 AT3G05610.1 38.566 516 282 13 26 510 54 565 6.79e-120 367
Msa0672090 AT5G04960.1 40.275 509 262 12 29 508 67 562 1.42e-118 360
Msa0672090 AT3G10710.1 41.797 512 260 13 24 510 63 561 6.32e-118 358
Msa0672090 AT5G20860.2 44.444 432 214 9 100 510 112 538 8.08e-118 357
Msa0672090 AT2G26440.1 40.504 516 271 11 28 510 35 547 6.74e-117 355
Msa0672090 AT3G10720.2 41.078 538 251 15 34 510 83 615 2.96e-116 356
Msa0672090 AT5G49180.1 39.810 525 259 16 28 506 53 566 1.10e-115 353
Msa0672090 AT5G27870.1 36.965 514 291 12 26 510 53 562 8.11e-114 353
Msa0672090 AT5G04970.1 40.074 539 255 15 34 510 88 620 2.22e-113 349
Msa0672090 AT4G33230.1 40.226 532 267 14 20 510 85 606 9.54e-112 344
Msa0672090 AT1G53840.1 38.846 520 266 14 28 504 71 581 4.75e-108 334
Msa0672090 AT3G06830.1 39.059 489 247 14 63 508 86 566 2.28e-107 332
Msa0672090 AT1G11590.1 37.736 530 297 11 7 510 2 524 2.94e-107 330
Msa0672090 AT4G03930.1 43.133 415 225 7 107 510 110 524 8.61e-106 326
Msa0672090 AT3G27980.1 43.284 402 202 6 125 510 106 497 1.58e-105 325
Msa0672090 AT3G14300.1 38.235 510 269 11 28 499 457 958 4.15e-103 331
Msa0672090 AT3G10720.1 57.692 260 106 3 254 510 1 259 5.55e-103 310
Msa0672090 AT4G15980.1 47.278 349 174 7 169 508 353 700 8.86e-101 318
Msa0672090 AT4G15980.2 47.278 349 174 7 169 508 363 710 2.49e-100 317
Msa0672090 AT5G64640.1 36.170 517 284 14 27 502 90 601 4.80e-96 303
Msa0672090 AT5G09760.1 36.346 509 281 14 28 498 43 546 2.40e-92 292
Msa0672090 AT5G09760.2 36.346 509 281 14 28 498 69 572 3.48e-92 292
Msa0672090 AT5G20860.1 40.955 398 182 8 100 476 112 477 4.37e-92 290
Msa0672090 AT2G47030.1 43.678 348 175 8 182 510 243 588 6.50e-82 266
Msa0672090 AT2G47040.1 43.768 345 176 8 182 510 253 595 1.53e-79 260
Msa0672090 AT3G62170.1 41.292 356 180 8 182 510 235 588 7.38e-78 255
Msa0672090 AT1G11370.1 49.187 246 119 4 203 442 43 288 1.16e-73 235
Msa0672090 AT5G19730.1 37.134 307 167 8 207 499 87 381 9.09e-48 170
Msa0672090 AT3G29090.1 35.789 285 162 5 201 472 2 278 9.38e-47 166
Msa0672090 AT1G05310.1 33.038 339 195 9 175 499 68 388 1.43e-43 159
Msa0672090 AT3G17060.1 32.492 317 187 9 195 499 35 336 1.81e-43 157
Msa0672090 AT2G36700.1 32.667 300 184 5 208 499 44 333 2.48e-43 157
Msa0672090 AT2G36710.1 32.704 318 185 10 203 504 86 390 3.71e-40 150
Msa0672090 AT1G44980.1 43.617 188 101 3 184 366 54 241 1.49e-39 144
Msa0672090 AT5G18990.1 33.798 287 164 7 201 472 24 299 9.06e-38 141
Msa0672090 AT2G21610.2 30.100 299 187 7 206 499 56 337 9.59e-38 142
Msa0672090 AT2G21610.1 30.100 299 187 7 206 499 51 332 1.06e-37 142
Msa0672090 AT5G55590.1 33.981 309 177 10 206 499 82 378 6.90e-37 140
Msa0672090 AT2G19150.1 31.889 323 190 10 198 506 33 339 5.27e-36 137
Msa0672090 AT3G24130.1 32.155 283 170 6 203 472 31 304 1.96e-35 135
Msa0672090 AT5G47500.1 28.267 329 198 8 186 499 52 357 3.75e-35 135
Msa0672090 AT5G07430.1 29.275 345 214 10 170 499 26 355 5.12e-35 135
Msa0672090 AT5G26810.1 30.793 328 197 10 192 501 20 335 1.14e-34 133
Msa0672090 AT5G55590.2 35.573 253 139 8 206 443 82 325 5.03e-34 131
Msa0672090 AT3G49220.2 30.961 281 166 7 29 282 131 410 2.11e-33 131
Msa0672090 AT2G47280.1 30.421 309 184 8 206 499 35 327 1.90e-30 121
Msa0672090 AT2G19150.2 32.558 258 151 8 198 443 33 279 4.63e-29 116
Msa0672090 AT5G07420.1 27.859 341 214 10 177 499 29 355 7.82e-29 117
Msa0672090 AT5G07410.1 26.087 345 228 10 168 499 25 355 3.58e-26 110
Msa0672090 AT5G61680.1 27.885 312 200 9 199 499 61 358 4.33e-26 109
Msa0672090 AT1G69940.1 25.946 370 244 12 145 499 1 355 2.61e-25 107

Find 124 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCTGTTCTATTGTATTT+AGG 0.188538 4_4:+16204471 Msa0672090:three_prime_UTR
TGTATTACTAGTTAGTTTAT+TGG 0.189679 4_4:+16204452 Msa0672090:three_prime_UTR
GGTTTGATTGAAAGCATTAT+TGG 0.202138 4_4:-16201204 None:intergenic
GGGACAGTTGATTTCATCTT+TGG 0.221019 4_4:+16203783 Msa0672090:CDS
GAATTATAATTCATAATTCT+AGG 0.221921 4_4:+16203913 Msa0672090:CDS
TATAGAGAATGTAACATCTA+TGG 0.255655 4_4:+16203762 Msa0672090:CDS
TTGGCTACACCATGATTTAA+TGG 0.264126 4_4:-16201147 None:intergenic
GAGAAGGGTTTATGAGGTTT+TGG 0.290145 4_4:-16200997 None:intergenic
TATGAACAATTCAGTATATA+TGG 0.307581 4_4:-16204350 None:intergenic
CAGGTTCATCCACTTCAAAT+AGG 0.323866 4_4:+16204126 Msa0672090:CDS
TTCATGAAAACTGTTATTGA+TGG 0.343065 4_4:+16204017 Msa0672090:CDS
AAGCTGTACTAAGCCATGTT+TGG 0.344214 4_4:-16201433 None:intergenic
TACTCACATGGTTGAGGATA+AGG 0.351224 4_4:-16201173 None:intergenic
CAAGCTGTTGCATTTCGTTC+CGG 0.356258 4_4:+16203660 Msa0672090:CDS
ACCGTTGGGAATTTCATTGC+CGG 0.366873 4_4:+16204203 Msa0672090:CDS
GAATATGCTAATACAGGTTC+AGG 0.371500 4_4:+16204107 Msa0672090:CDS
AGCTGTACTAAGCCATGTTT+GGG 0.379379 4_4:-16201432 None:intergenic
ATTTCATTGCCGGTAATTCA+TGG 0.379520 4_4:+16204213 Msa0672090:CDS
CATCTTTGTAACTTGGTTGT+TGG 0.388751 4_4:-16201583 None:intergenic
CTGGACTTTAAGCGTTCCTT+TGG 0.395121 4_4:+16204264 Msa0672090:exon
GTTTCCTATCCATGAATTAC+CGG 0.395370 4_4:-16204222 None:intergenic
AACTTAGAGAAGGGTTTATG+AGG 0.395587 4_4:-16201003 None:intergenic
ACTCACATGGTTGAGGATAA+GGG 0.396273 4_4:-16201172 None:intergenic
ACAATAGTGGAGAAAAGAAA+TGG 0.403364 4_4:-16201104 None:intergenic
ATGGTTGAGGATAAGGGGTT+TGG 0.413369 4_4:-16201166 None:intergenic
TCTTGTGGGCTACGGCACTT+AGG 0.413550 4_4:-16201317 None:intergenic
CGTCGTTGCTAAGGATGGTT+CGG 0.414368 4_4:+16201679 Msa0672090:CDS
TTGTAACTTGGTTGTTGGTA+AGG 0.414505 4_4:-16201578 None:intergenic
TTCTAGGGTTTCCGCTCAAT+CGG 0.419321 4_4:+16203929 Msa0672090:CDS
AGGTTCATCCACTTCAAATA+GGG 0.421888 4_4:+16204127 Msa0672090:CDS
TTTGCAGCTGCTACTGGTGA+TGG 0.423826 4_4:+16203585 Msa0672090:intron
GTCGTTGCTAAGGATGGTTC+GGG 0.434190 4_4:+16201680 Msa0672090:CDS
GAATTGTTCATAAGATCAAT+TGG 0.440829 4_4:+16204361 Msa0672090:three_prime_UTR
TTATTTAAAAGAATTGTTGT+TGG 0.441698 4_4:+16204550 Msa0672090:three_prime_UTR
CATGGGTTAAACCTGGTGAT+AGG 0.443867 4_4:+16201615 Msa0672090:CDS
AGTTTCACTTGAACTTGCTA+TGG 0.444556 4_4:+16201253 Msa0672090:CDS
GGCTAATGTCGTCGTTGCTA+AGG 0.459522 4_4:+16201670 Msa0672090:CDS
CCACTTCAAATAGGGTTACA+TGG 0.460645 4_4:+16204135 Msa0672090:CDS
CTTGTGGGCTACGGCACTTA+GGG 0.460985 4_4:-16201316 None:intergenic
TATGCTGAATATGCTAATAC+AGG 0.469023 4_4:+16204101 Msa0672090:CDS
CATTAAGTTCCATTAAATCA+TGG 0.471538 4_4:+16201138 Msa0672090:CDS
CAAGCTTCACCTTTCACCGT+TGG 0.471593 4_4:+16204188 Msa0672090:CDS
AAGCTTCACCTTTCACCGTT+GGG 0.472202 4_4:+16204189 Msa0672090:CDS
ATGAAATTCCCAACGGTGAA+AGG 0.479388 4_4:-16204197 None:intergenic
CAACAACCAAGTTACAAAGA+TGG 0.480845 4_4:+16201584 Msa0672090:CDS
CCCTGCTGGTTGGTTACCAT+GGG 0.482029 4_4:+16204049 Msa0672090:CDS
AATTATAATTCATAATTCTA+GGG 0.485667 4_4:+16203914 Msa0672090:CDS
ACAGGCGTGCCATTCACCTC+TGG 0.485673 4_4:+16204245 Msa0672090:CDS
CTGTTAAATCGTACCTTGGT+AGG 0.486089 4_4:+16203967 Msa0672090:CDS
ATAGAGAATGTAACATCTAT+GGG 0.487737 4_4:+16203763 Msa0672090:CDS
TGAGTAACCATAAACAATAG+TGG 0.489662 4_4:-16201117 None:intergenic
CCATGTAACCCTATTTGAAG+TGG 0.490411 4_4:-16204135 None:intergenic
AACTTCTTCCGCTGCTTCGA+AGG 0.490876 4_4:+16201649 Msa0672090:CDS
GCAAAGAATGTCATGTTGGT+TGG 0.492347 4_4:+16201809 Msa0672090:CDS
GGAAATCCATCTTTGTAACT+TGG 0.499245 4_4:-16201590 None:intergenic
ATTGCCGGTAATTCATGGAT+AGG 0.500552 4_4:+16204218 Msa0672090:CDS
TCCCATGGTAACCAACCAGC+AGG 0.502238 4_4:-16204050 None:intergenic
TCCTTGTGCGGTAACTGTGA+TGG 0.506939 4_4:-16203865 None:intergenic
ACCCTGCTGGTTGGTTACCA+TGG 0.509749 4_4:+16204048 Msa0672090:CDS
TAACTAGTAATACATTTGAA+TGG 0.513519 4_4:-16204443 None:intergenic
GGTACGATTTAACAGAGCTA+GGG 0.515662 4_4:-16203959 None:intergenic
GATGCTGCACCAAGTGGTAG+TGG 0.515780 4_4:+16201731 Msa0672090:CDS
ATATAAAGGCACTAAAATCA+AGG 0.525610 4_4:+16200950 None:intergenic
TCAAATAGGGTTACATGGAA+AGG 0.533882 4_4:+16204140 Msa0672090:CDS
AGGTACGATTTAACAGAGCT+AGG 0.534156 4_4:-16203960 None:intergenic
AAATAATACTCACATGGTTG+AGG 0.534999 4_4:-16201179 None:intergenic
ATTGGTAAAACCATAATCAC+TGG 0.538242 4_4:+16201839 Msa0672090:CDS
TCACCAGGTTTAACCCATGT+TGG 0.540325 4_4:-16201611 None:intergenic
TGAAGAAGTTTCCTATCACC+AGG 0.540939 4_4:-16201626 None:intergenic
ATGATGAGAAACTTAGAGAA+GGG 0.541566 4_4:-16201012 None:intergenic
ATCACTGGTAGCAAAAGTGT+TGG 0.542034 4_4:+16201854 Msa0672090:CDS
CTCACATGGTTGAGGATAAG+GGG 0.542503 4_4:-16201171 None:intergenic
TCGTTCCGGCTCAGATCTAT+CGG 0.547689 4_4:+16203674 Msa0672090:CDS
TAAAGCAAAGAATGTCATGT+TGG 0.552893 4_4:+16201805 Msa0672090:CDS
ATGTTGGTTGGAGATGGTAT+TGG 0.553011 4_4:+16201821 Msa0672090:CDS
AAATACCGATAGATCTGAGC+CGG 0.556401 4_4:-16203679 None:intergenic
ACAAAGATGGATTTCCAACA+TGG 0.557740 4_4:+16201597 Msa0672090:CDS
ACCATCACAGTTACCGCACA+AGG 0.559866 4_4:+16203864 Msa0672090:CDS
AATGTCGTCGTTGCTAAGGA+TGG 0.559942 4_4:+16201674 Msa0672090:CDS
ACTGGTAGCAAAAGTGTTGG+AGG 0.563435 4_4:+16201857 Msa0672090:CDS
ATCACATTCAGAAACACAGC+TGG 0.566335 4_4:+16203624 Msa0672090:CDS
ATTATAAGAGTTGTTACCAA+AGG 0.567706 4_4:-16201077 None:intergenic
ACCGATCCAAATCAAAACAC+TGG 0.569193 4_4:+16203891 Msa0672090:CDS
AATCGTACCTTGGTAGGCCG+TGG 0.576253 4_4:+16203973 Msa0672090:CDS
CACAATATGAATCCCAAACA+TGG 0.578929 4_4:+16201420 Msa0672090:CDS
GCTTAAAGTCCAGAGGTGAA+TGG 0.586097 4_4:-16204254 None:intergenic
TTTCCAACATGGGTTAAACC+TGG 0.589586 4_4:+16201608 Msa0672090:CDS
AATGTCATGTTGGTTGGAGA+TGG 0.590411 4_4:+16201815 Msa0672090:CDS
TGGATCGGTTCTTCCTTGTG+CGG 0.593989 4_4:-16203877 None:intergenic
GTTACATTATTAGACAACAA+AGG 0.594044 4_4:-16201518 None:intergenic
ACTTTCCGTTCAGCAACCGT+TGG 0.594941 4_4:+16201890 Msa0672090:CDS
TATGATGAGAAACTTAGAGA+AGG 0.598833 4_4:-16201013 None:intergenic
TATGTGATATATGTGAAGGC+TGG 0.599695 4_4:+16201758 Msa0672090:CDS
GGGCAAGTACACGACGGTGA+AGG 0.602626 4_4:+16201700 Msa0672090:CDS
ATAAATTGACGCGATTCCAA+AGG 0.602880 4_4:-16204280 None:intergenic
GCTGGTTGGTTACCATGGGA+TGG 0.604546 4_4:+16204053 Msa0672090:CDS
CTGCACCAAGTGGTAGTGGA+AGG 0.608150 4_4:+16201735 Msa0672090:CDS
AAAGCAAAATTACCATCCCA+TGG 0.612268 4_4:-16204065 None:intergenic
CACGACGGTGAAGGCCGCGA+CGG 0.612289 4_4:+16201709 Msa0672090:CDS
GACATTAGCCTTCGAAGCAG+CGG 0.622925 4_4:-16201657 None:intergenic
TAACACCTTGTCACTCAACA+AGG 0.624473 4_4:+16201553 Msa0672090:CDS
AGCTCTGTTAAATCGTACCT+TGG 0.625217 4_4:+16203963 Msa0672090:CDS
ACTTACCAACGGTTGCTGAA+CGG 0.626940 4_4:-16201895 None:intergenic
CAAAGATGGATTTCCAACAT+GGG 0.627787 4_4:+16201598 Msa0672090:CDS
AGGGTTTAAATCCGATTGAG+CGG 0.635907 4_4:-16203940 None:intergenic
ACATACCTTCCACTACCACT+TGG 0.638708 4_4:-16201740 None:intergenic
GGTAGCAAAAGTGTTGGAGG+TGG 0.640198 4_4:+16201860 Msa0672090:CDS
GTGAAGCACTAGTAAGAACA+TGG 0.641780 4_4:-16204166 None:intergenic
GAAGAAAATACACATGCACT+TGG 0.644990 4_4:+16201293 Msa0672090:CDS
TTGGTTAAATAATACTCACA+TGG 0.648130 4_4:-16201185 None:intergenic
AAGGTATGTGATATATGTGA+AGG 0.653633 4_4:+16201754 Msa0672090:CDS
AGGAACCTTGTTGAGTGACA+AGG 0.656613 4_4:-16201558 None:intergenic
GCGACGGATGCTGCACCAAG+TGG 0.664715 4_4:+16201725 Msa0672090:CDS
GGTTGCTGAACGGAAAGTAG+TGG 0.670011 4_4:-16201885 None:intergenic
CCCATGGTAACCAACCAGCA+GGG 0.670836 4_4:-16204049 None:intergenic
ACCGGCAATGAAATTCCCAA+CGG 0.671958 4_4:-16204204 None:intergenic
TTAATTGAGAACTAATCACA+AGG 0.685708 4_4:-16204388 None:intergenic
AATTCATGGATAGGAAACAC+AGG 0.687418 4_4:+16204227 Msa0672090:CDS
AAGGAACGCTTAAAGTCCAG+AGG 0.689657 4_4:-16204261 None:intergenic
ACTTGGTGCAGCATCCGTCG+CGG 0.690402 4_4:-16201723 None:intergenic
AGACATAGTTTGTGACACCA+AGG 0.696122 4_4:-16201493 None:intergenic
TCAATTTATCAGAATCACAG+TGG 0.728182 4_4:+16204294 Msa0672090:three_prime_UTR
TGGTTCGGGCAAGTACACGA+CGG 0.758627 4_4:+16201694 Msa0672090:CDS
TTTCATCTTTGGAAACGCCG+CGG 0.774990 4_4:+16203794 Msa0672090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAATATTTAAATTAA+TGG - chr4_4:16202236-16202255 None:intergenic 0.0%
!! AAATATAACAAATAACTTAA+AGG - chr4_4:16202387-16202406 None:intergenic 10.0%
!! AAATAAATAATTATATCAGT+AGG + chr4_4:16202509-16202528 Msa0672090:intron 10.0%
!! AATAAATAATTATATCAGTA+GGG + chr4_4:16202510-16202529 Msa0672090:intron 10.0%
!! TTTCTTAATCATTAAATTAA+GGG - chr4_4:16202855-16202874 None:intergenic 10.0%
!! ATTTCTTAATCATTAAATTA+AGG - chr4_4:16202856-16202875 None:intergenic 10.0%
!! AATTATAATTCATAATTCTA+GGG + chr4_4:16203914-16203933 Msa0672090:CDS 10.0%
!!! TAACAATACAATTAATTTTT+TGG - chr4_4:16204519-16204538 None:intergenic 10.0%
!!! AAAATCGGATTTATTTTTTA+TGG - chr4_4:16201228-16201247 None:intergenic 15.0%
!!! TATTTACAAAAAAAAAGTGT+TGG + chr4_4:16202345-16202364 Msa0672090:intron 15.0%
!! ATAAATAATTATATCAGTAG+GGG + chr4_4:16202511-16202530 Msa0672090:intron 15.0%
!! GAATAATTATTTATATCCTT+TGG - chr4_4:16202721-16202740 None:intergenic 15.0%
!!! ATTACTTTAAAATCTCTTTA+CGG + chr4_4:16202765-16202784 Msa0672090:intron 15.0%
!! TTAAGATAATATATCATAGA+AGG - chr4_4:16202792-16202811 None:intergenic 15.0%
!! TTCAATTAAATCAATTATTG+TGG - chr4_4:16202887-16202906 None:intergenic 15.0%
!!! ATTCAATTCATTATGTTTTT+GGG - chr4_4:16202923-16202942 None:intergenic 15.0%
!!! TATTCAATTCATTATGTTTT+TGG - chr4_4:16202924-16202943 None:intergenic 15.0%
!! TTAAGGAAAAATATTAATCT+AGG - chr4_4:16203178-16203197 None:intergenic 15.0%
!! ACTTTAACTAATTTCTTAAT+TGG + chr4_4:16203230-16203249 Msa0672090:intron 15.0%
!! GAATAAGAATATATTAAAGT+GGG - chr4_4:16203297-16203316 None:intergenic 15.0%
!! TGAATAAGAATATATTAAAG+TGG - chr4_4:16203298-16203317 None:intergenic 15.0%
!! TTTAATTAAAAGAAGAATGT+GGG + chr4_4:16203329-16203348 Msa0672090:intron 15.0%
!! GAATTATAATTCATAATTCT+AGG + chr4_4:16203913-16203932 Msa0672090:CDS 15.0%
!! GAAAGAAAAATAATTTAGTA+CGG + chr4_4:16204493-16204512 Msa0672090:three_prime_UTR 15.0%
!! TTATTTAAAAGAATTGTTGT+TGG + chr4_4:16204550-16204569 Msa0672090:three_prime_UTR 15.0%
!!! AAAATTATTTTAGGGTATGA+TGG - chr4_4:16201039-16201058 None:intergenic 20.0%
!!! ACCCTAAAATAATTTTTCAA+TGG + chr4_4:16201043-16201062 Msa0672090:exon 20.0%
!!! GCCATTGAAAAATTATTTTA+GGG - chr4_4:16201047-16201066 None:intergenic 20.0%
!!! AGCCATTGAAAAATTATTTT+AGG - chr4_4:16201048-16201067 None:intergenic 20.0%
!!! AAGTGAAACTTTGAAAAAAT+CGG - chr4_4:16201243-16201262 None:intergenic 20.0%
!!! TGAATGAACTTTTTTTTTCT+TGG - chr4_4:16202050-16202069 None:intergenic 20.0%
!! CTAGTTTATGATTTAGTTAT+TGG - chr4_4:16202082-16202101 None:intergenic 20.0%
!!! TGCATAAGATTTTTCAATAA+AGG - chr4_4:16202112-16202131 None:intergenic 20.0%
!! GAAAATTACTTATGAATGAT+AGG + chr4_4:16202271-16202290 Msa0672090:intron 20.0%
!!! TACTTATTTTATTTCCATCT+AGG + chr4_4:16202305-16202324 Msa0672090:intron 20.0%
!!! ACTTATTTTATTTCCATCTA+GGG + chr4_4:16202306-16202325 Msa0672090:intron 20.0%
!! AAAATTAATTTAACCCTAGA+TGG - chr4_4:16202322-16202341 None:intergenic 20.0%
!!! AGATTTTAAAGTAATCACAA+TGG - chr4_4:16202760-16202779 None:intergenic 20.0%
!! ATGTAAATGATTCAAATCAT+AGG - chr4_4:16202983-16203002 None:intergenic 20.0%
!! TATGATTTGAATCATTTACA+TGG + chr4_4:16202982-16203001 Msa0672090:intron 20.0%
!! TGACTACTAAAAATCTTATT+TGG - chr4_4:16203085-16203104 None:intergenic 20.0%
!!! CACATTCTTCTTTTAATTAA+AGG - chr4_4:16203330-16203349 None:intergenic 20.0%
!! CTTTAATTAAAAGAAGAATG+TGG + chr4_4:16203328-16203347 Msa0672090:intron 20.0%
!! TTAATTAAAAGAAGAATGTG+GGG + chr4_4:16203330-16203349 Msa0672090:intron 20.0%
!! TTCAACTGATTATTAACTTA+TGG - chr4_4:16203373-16203392 None:intergenic 20.0%
!! TATGAACAATTCAGTATATA+TGG - chr4_4:16204353-16204372 None:intergenic 20.0%
!! TAACTAGTAATACATTTGAA+TGG - chr4_4:16204446-16204465 None:intergenic 20.0%
!!! TGTATTACTAGTTAGTTTAT+TGG + chr4_4:16204452-16204471 Msa0672090:three_prime_UTR 20.0%
! ATTATAAGAGTTGTTACCAA+AGG - chr4_4:16201080-16201099 None:intergenic 25.0%
! CATTAAGTTCCATTAAATCA+TGG + chr4_4:16201138-16201157 Msa0672090:CDS 25.0%
! TTGGTTAAATAATACTCACA+TGG - chr4_4:16201188-16201207 None:intergenic 25.0%
!!! AAAAATGGATTTTATGACCT+TGG + chr4_4:16201476-16201495 Msa0672090:CDS 25.0%
! GTTACATTATTAGACAACAA+AGG - chr4_4:16201521-16201540 None:intergenic 25.0%
!! CTTTAAGGGATAAACTTTAA+GGG - chr4_4:16201962-16201981 None:intergenic 25.0%
!! TCTTTAAGGGATAAACTTTA+AGG - chr4_4:16201963-16201982 None:intergenic 25.0%
!! ATTTGCGAACTTTTCTTTAA+GGG - chr4_4:16201976-16201995 None:intergenic 25.0%
!! TATTTGCGAACTTTTCTTTA+AGG - chr4_4:16201977-16201996 None:intergenic 25.0%
!! CAGGTAAGAACTTTTTAATA+AGG - chr4_4:16202021-16202040 None:intergenic 25.0%
! ACTTATGAATGATAGGTAAA+CGG + chr4_4:16202278-16202297 Msa0672090:intron 25.0%
! ATTATCTACTTTCTTACTCA+AGG - chr4_4:16202695-16202714 None:intergenic 25.0%
!!! CTGCTTGCTTTTATATAATT+AGG - chr4_4:16202816-16202835 None:intergenic 25.0%
! ACATAATGAATTGAATATCG+TGG + chr4_4:16202927-16202946 Msa0672090:intron 25.0%
! ATCATAGGCTAAAACTTTAT+TGG - chr4_4:16202968-16202987 None:intergenic 25.0%
! TATGTAGCTTTCAAAAATGA+AGG + chr4_4:16203021-16203040 Msa0672090:intron 25.0%
! TTTAGTAGTCAAATCTTTAG+AGG + chr4_4:16203094-16203113 Msa0672090:intron 25.0%
! TATAGAGAATGTAACATCTA+TGG + chr4_4:16203762-16203781 Msa0672090:CDS 25.0%
! ATAGAGAATGTAACATCTAT+GGG + chr4_4:16203763-16203782 Msa0672090:CDS 25.0%
!!! ATAATTCCAGTGTTTTGATT+TGG - chr4_4:16203900-16203919 None:intergenic 25.0%
! TTCATGAAAACTGTTATTGA+TGG + chr4_4:16204017-16204036 Msa0672090:CDS 25.0%
! GAATTGTTCATAAGATCAAT+TGG + chr4_4:16204361-16204380 Msa0672090:three_prime_UTR 25.0%
! TTAATTGAGAACTAATCACA+AGG - chr4_4:16204391-16204410 None:intergenic 25.0%
!!! TTTTGGTTTGATTATGAGTG+AGG - chr4_4:16200983-16201002 None:intergenic 30.0%
ATGATGAGAAACTTAGAGAA+GGG - chr4_4:16201015-16201034 None:intergenic 30.0%
TATGATGAGAAACTTAGAGA+AGG - chr4_4:16201016-16201035 None:intergenic 30.0%
!!! AATGGCTTCATTTTTTCCTT+TGG + chr4_4:16201061-16201080 Msa0672090:exon 30.0%
! ACAATAGTGGAGAAAAGAAA+TGG - chr4_4:16201107-16201126 None:intergenic 30.0%
! CTTTTCTCCACTATTGTTTA+TGG + chr4_4:16201110-16201129 Msa0672090:CDS 30.0%
TGAGTAACCATAAACAATAG+TGG - chr4_4:16201120-16201139 None:intergenic 30.0%
AAATAATACTCACATGGTTG+AGG - chr4_4:16201182-16201201 None:intergenic 30.0%
!! GGTTTGATTGAAAGCATTAT+TGG - chr4_4:16201207-16201226 None:intergenic 30.0%
!! ATTTTGTTTGGCTAAGCTTT+TGG - chr4_4:16201388-16201407 None:intergenic 30.0%
! TGCACTTTGTGTATTTTGTT+TGG - chr4_4:16201400-16201419 None:intergenic 30.0%
AACCTTGAAACATGCAAAAA+TGG + chr4_4:16201461-16201480 Msa0672090:CDS 30.0%
!! ATCCATTTTTGCATGTTTCA+AGG - chr4_4:16201466-16201485 None:intergenic 30.0%
AAGGTATGTGATATATGTGA+AGG + chr4_4:16201754-16201773 Msa0672090:CDS 30.0%
TAAAGCAAAGAATGTCATGT+TGG + chr4_4:16201805-16201824 Msa0672090:CDS 30.0%
ATTGGTAAAACCATAATCAC+TGG + chr4_4:16201839-16201858 Msa0672090:CDS 30.0%
!! TTACTAGAAGTACTTACCAA+CGG - chr4_4:16201909-16201928 None:intergenic 30.0%
AAGGGATAAAAAAAGCTGTA+CGG - chr4_4:16201944-16201963 None:intergenic 30.0%
!!! TTTTTTTTCTTGGTCAAGAC+AGG - chr4_4:16202040-16202059 None:intergenic 30.0%
TGCTTAGAAAGTTAACTAGT+CGG + chr4_4:16202139-16202158 Msa0672090:intron 30.0%
CTTATGAATGATAGGTAAAC+GGG + chr4_4:16202279-16202298 Msa0672090:intron 30.0%
TTATGAATGATAGGTAAACG+GGG + chr4_4:16202280-16202299 Msa0672090:intron 30.0%
TGAGAAGCTATGTATAACAA+CGG + chr4_4:16202555-16202574 Msa0672090:intron 30.0%
!! GTATTGACTTTAGATTTGTG+TGG + chr4_4:16202631-16202650 Msa0672090:intron 30.0%
CTTTAGATTTGTGTGGTATA+TGG + chr4_4:16202638-16202657 Msa0672090:intron 30.0%
GAAAGTAGATAATAGACCAA+AGG + chr4_4:16202702-16202721 Msa0672090:intron 30.0%
GGCTTTCTATATGTTATAGT+CGG - chr4_4:16203128-16203147 None:intergenic 30.0%
! TAGGATGATTTATTCTAGAG+GGG - chr4_4:16203159-16203178 None:intergenic 30.0%
CTAGGATGATTTATTCTAGA+GGG - chr4_4:16203160-16203179 None:intergenic 30.0%
! TCTAGGATGATTTATTCTAG+AGG - chr4_4:16203161-16203180 None:intergenic 30.0%
CACATAGGAAACTATATATG+CGG - chr4_4:16203205-16203224 None:intergenic 30.0%
! TAGTTAAAGTGTTACCACAT+AGG - chr4_4:16203220-16203239 None:intergenic 30.0%
! TTTGGATTGGAATCTACAAT+TGG + chr4_4:16203428-16203447 Msa0672090:intron 30.0%
! TTTTACAAATGTAGCTTCGA+AGG + chr4_4:16203699-16203718 Msa0672090:CDS 30.0%
!! GTTCTTGAATATTTTTGCCA+CGG - chr4_4:16203993-16204012 None:intergenic 30.0%
TATGCTGAATATGCTAATAC+AGG + chr4_4:16204101-16204120 Msa0672090:CDS 30.0%
TCAATTTATCAGAATCACAG+TGG + chr4_4:16204294-16204313 Msa0672090:three_prime_UTR 30.0%
TTGGCTGTTCTATTGTATTT+AGG + chr4_4:16204471-16204490 Msa0672090:three_prime_UTR 30.0%
AACTTAGAGAAGGGTTTATG+AGG - chr4_4:16201006-16201025 None:intergenic 35.0%
TTGGCTACACCATGATTTAA+TGG - chr4_4:16201150-16201169 None:intergenic 35.0%
!! AGTTTCACTTGAACTTGCTA+TGG + chr4_4:16201253-16201272 Msa0672090:CDS 35.0%
GAAGAAAATACACATGCACT+TGG + chr4_4:16201293-16201312 Msa0672090:CDS 35.0%
CACAATATGAATCCCAAACA+TGG + chr4_4:16201420-16201439 Msa0672090:CDS 35.0%
TTGTAACTTGGTTGTTGGTA+AGG - chr4_4:16201581-16201600 None:intergenic 35.0%
CATCTTTGTAACTTGGTTGT+TGG - chr4_4:16201586-16201605 None:intergenic 35.0%
CAACAACCAAGTTACAAAGA+TGG + chr4_4:16201584-16201603 Msa0672090:CDS 35.0%
GGAAATCCATCTTTGTAACT+TGG - chr4_4:16201593-16201612 None:intergenic 35.0%
! ACAAAGATGGATTTCCAACA+TGG + chr4_4:16201597-16201616 Msa0672090:CDS 35.0%
! CAAAGATGGATTTCCAACAT+GGG + chr4_4:16201598-16201617 Msa0672090:CDS 35.0%
TATGTGATATATGTGAAGGC+TGG + chr4_4:16201758-16201777 Msa0672090:CDS 35.0%
! ACTTTTGCTACCAGTGATTA+TGG - chr4_4:16201852-16201871 None:intergenic 35.0%
TACATAGCTTCTCATTCTAG+AGG - chr4_4:16202549-16202568 None:intergenic 35.0%
AAGATATGCAACCTTAACAC+CGG - chr4_4:16202591-16202610 None:intergenic 35.0%
!!! TGTTTTTGGGCTCAATTATG+TGG - chr4_4:16202910-16202929 None:intergenic 35.0%
CACATGTATTGTCTTACGTT+TGG + chr4_4:16203046-16203065 Msa0672090:intron 35.0%
ACTATATATGCGGTGCATTA+AGG - chr4_4:16203195-16203214 None:intergenic 35.0%
CGCATATATAGTTTCCTATG+TGG + chr4_4:16203203-16203222 Msa0672090:intron 35.0%
TATTAAAGTGGGTCCTACAT+TGG - chr4_4:16203286-16203305 None:intergenic 35.0%
GAGTATTAAAAAACCTAGCG+TGG - chr4_4:16203551-16203570 None:intergenic 35.0%
! ACAATTTTGCAAAACTACCG+CGG - chr4_4:16203814-16203833 None:intergenic 35.0%
!!! TCCAGTGTTTTGATTTGGAT+CGG - chr4_4:16203895-16203914 None:intergenic 35.0%
AAAGCAAAATTACCATCCCA+TGG - chr4_4:16204068-16204087 None:intergenic 35.0%
! ATGGGATGGTAATTTTGCTT+TGG + chr4_4:16204067-16204086 Msa0672090:CDS 35.0%
GAATATGCTAATACAGGTTC+AGG + chr4_4:16204107-16204126 Msa0672090:CDS 35.0%
AGGTTCATCCACTTCAAATA+GGG + chr4_4:16204127-16204146 Msa0672090:CDS 35.0%
TCAAATAGGGTTACATGGAA+AGG + chr4_4:16204140-16204159 Msa0672090:CDS 35.0%
ATTTCATTGCCGGTAATTCA+TGG + chr4_4:16204213-16204232 Msa0672090:CDS 35.0%
GTTTCCTATCCATGAATTAC+CGG - chr4_4:16204225-16204244 None:intergenic 35.0%
AATTCATGGATAGGAAACAC+AGG + chr4_4:16204227-16204246 Msa0672090:CDS 35.0%
ATAAATTGACGCGATTCCAA+AGG - chr4_4:16204283-16204302 None:intergenic 35.0%
! GAGAAGGGTTTATGAGGTTT+TGG - chr4_4:16201000-16201019 None:intergenic 40.0%
ACTCACATGGTTGAGGATAA+GGG - chr4_4:16201175-16201194 None:intergenic 40.0%
TACTCACATGGTTGAGGATA+AGG - chr4_4:16201176-16201195 None:intergenic 40.0%
GCTATGGAGAGAGCAAAAAA+AGG + chr4_4:16201269-16201288 Msa0672090:CDS 40.0%
AGCTGTACTAAGCCATGTTT+GGG - chr4_4:16201435-16201454 None:intergenic 40.0%
AAGCTGTACTAAGCCATGTT+TGG - chr4_4:16201436-16201455 None:intergenic 40.0%
! AGACATAGTTTGTGACACCA+AGG - chr4_4:16201496-16201515 None:intergenic 40.0%
TAACACCTTGTCACTCAACA+AGG + chr4_4:16201553-16201572 Msa0672090:CDS 40.0%
! TTTCCAACATGGGTTAAACC+TGG + chr4_4:16201608-16201627 Msa0672090:CDS 40.0%
TGAAGAAGTTTCCTATCACC+AGG - chr4_4:16201629-16201648 None:intergenic 40.0%
GCAAAGAATGTCATGTTGGT+TGG + chr4_4:16201809-16201828 Msa0672090:CDS 40.0%
! AATGTCATGTTGGTTGGAGA+TGG + chr4_4:16201815-16201834 Msa0672090:CDS 40.0%
! ATGTTGGTTGGAGATGGTAT+TGG + chr4_4:16201821-16201840 Msa0672090:CDS 40.0%
! ATCACTGGTAGCAAAAGTGT+TGG + chr4_4:16201854-16201873 Msa0672090:CDS 40.0%
TGTATAACAACGGTCCTTCT+CGG + chr4_4:16202565-16202584 Msa0672090:intron 40.0%
GTATTGTCTTACGTTTGGCT+AGG + chr4_4:16203051-16203070 Msa0672090:intron 40.0%
! TATTCTAGAGGGGAAAGATG+TGG - chr4_4:16203149-16203168 None:intergenic 40.0%
CACAATTCCACATCCAATGT+AGG + chr4_4:16203270-16203289 Msa0672090:intron 40.0%
! AGTACACACGTCATGCATTT+TGG + chr4_4:16203410-16203429 Msa0672090:intron 40.0%
! ACACGTCATGCATTTTGGAT+TGG + chr4_4:16203415-16203434 Msa0672090:intron 40.0%
!!! TTGTTTTTTGCAGCTGCTAC+TGG + chr4_4:16203579-16203598 Msa0672090:intron 40.0%
ATCACATTCAGAAACACAGC+TGG + chr4_4:16203624-16203643 Msa0672090:CDS 40.0%
AAATACCGATAGATCTGAGC+CGG - chr4_4:16203682-16203701 None:intergenic 40.0%
! GGGACAGTTGATTTCATCTT+TGG + chr4_4:16203783-16203802 Msa0672090:CDS 40.0%
! GTAACTGTGATGGTTTTTGC+AGG - chr4_4:16203858-16203877 None:intergenic 40.0%
ACCGATCCAAATCAAAACAC+TGG + chr4_4:16203891-16203910 Msa0672090:CDS 40.0%
AGGGTTTAAATCCGATTGAG+CGG - chr4_4:16203943-16203962 None:intergenic 40.0%
GGTACGATTTAACAGAGCTA+GGG - chr4_4:16203962-16203981 None:intergenic 40.0%
AGGTACGATTTAACAGAGCT+AGG - chr4_4:16203963-16203982 None:intergenic 40.0%
AGCTCTGTTAAATCGTACCT+TGG + chr4_4:16203963-16203982 Msa0672090:CDS 40.0%
CTGTTAAATCGTACCTTGGT+AGG + chr4_4:16203967-16203986 Msa0672090:CDS 40.0%
!!! GATGGTTTTATTAACCCTGC+TGG + chr4_4:16204035-16204054 Msa0672090:CDS 40.0%
!! GTTTTATTAACCCTGCTGGT+TGG + chr4_4:16204039-16204058 Msa0672090:CDS 40.0%
CAGGTTCATCCACTTCAAAT+AGG + chr4_4:16204126-16204145 Msa0672090:CDS 40.0%
CCATGTAACCCTATTTGAAG+TGG - chr4_4:16204138-16204157 None:intergenic 40.0%
CCACTTCAAATAGGGTTACA+TGG + chr4_4:16204135-16204154 Msa0672090:CDS 40.0%
! GTGAAGCACTAGTAAGAACA+TGG - chr4_4:16204169-16204188 None:intergenic 40.0%
ATGAAATTCCCAACGGTGAA+AGG - chr4_4:16204200-16204219 None:intergenic 40.0%
ATTGCCGGTAATTCATGGAT+AGG + chr4_4:16204218-16204237 Msa0672090:CDS 40.0%
ATGGTTGAGGATAAGGGGTT+TGG - chr4_4:16201169-16201188 None:intergenic 45.0%
CTCACATGGTTGAGGATAAG+GGG - chr4_4:16201174-16201193 None:intergenic 45.0%
!! CAGCTTTTTCTTGTGGGCTA+CGG - chr4_4:16201328-16201347 None:intergenic 45.0%
!! CCCAAGCAGCTTTTTCTTGT+GGG - chr4_4:16201334-16201353 None:intergenic 45.0%
CCCACAAGAAAAAGCTGCTT+GGG + chr4_4:16201331-16201350 Msa0672090:CDS 45.0%
AGGAACCTTGTTGAGTGACA+AGG - chr4_4:16201561-16201580 None:intergenic 45.0%
TCACCAGGTTTAACCCATGT+TGG - chr4_4:16201614-16201633 None:intergenic 45.0%
! CATGGGTTAAACCTGGTGAT+AGG + chr4_4:16201615-16201634 Msa0672090:CDS 45.0%
AATGTCGTCGTTGCTAAGGA+TGG + chr4_4:16201674-16201693 Msa0672090:CDS 45.0%
ACATACCTTCCACTACCACT+TGG - chr4_4:16201743-16201762 None:intergenic 45.0%
!! ACTGGTAGCAAAAGTGTTGG+AGG + chr4_4:16201857-16201876 Msa0672090:CDS 45.0%
ACTTACCAACGGTTGCTGAA+CGG - chr4_4:16201898-16201917 None:intergenic 45.0%
TCTTACGTTTGGCTAGGACA+AGG + chr4_4:16203057-16203076 Msa0672090:intron 45.0%
CAAGCTGTTGCATTTCGTTC+CGG + chr4_4:16203660-16203679 Msa0672090:CDS 45.0%
! TTTCATCTTTGGAAACGCCG+CGG + chr4_4:16203794-16203813 Msa0672090:CDS 45.0%
! ACTGTGATGGTTTTTGCAGG+CGG - chr4_4:16203855-16203874 None:intergenic 45.0%
!! TTCTAGGGTTTCCGCTCAAT+CGG + chr4_4:16203929-16203948 Msa0672090:CDS 45.0%
AAGCTTCACCTTTCACCGTT+GGG + chr4_4:16204189-16204208 Msa0672090:CDS 45.0%
ACCGTTGGGAATTTCATTGC+CGG + chr4_4:16204203-16204222 Msa0672090:CDS 45.0%
ACCGGCAATGAAATTCCCAA+CGG - chr4_4:16204207-16204226 None:intergenic 45.0%
GCTTAAAGTCCAGAGGTGAA+TGG - chr4_4:16204257-16204276 None:intergenic 45.0%
AAGGAACGCTTAAAGTCCAG+AGG - chr4_4:16204264-16204283 None:intergenic 45.0%
CTGGACTTTAAGCGTTCCTT+TGG + chr4_4:16204264-16204283 Msa0672090:exon 45.0%
GCCCACAAGAAAAAGCTGCT+TGG + chr4_4:16201330-16201349 Msa0672090:CDS 50.0%
!! GCCCAAGCAGCTTTTTCTTG+TGG - chr4_4:16201335-16201354 None:intergenic 50.0%
AACTTCTTCCGCTGCTTCGA+AGG + chr4_4:16201649-16201668 Msa0672090:CDS 50.0%
!! GACATTAGCCTTCGAAGCAG+CGG - chr4_4:16201660-16201679 None:intergenic 50.0%
GGCTAATGTCGTCGTTGCTA+AGG + chr4_4:16201670-16201689 Msa0672090:CDS 50.0%
CGTCGTTGCTAAGGATGGTT+CGG + chr4_4:16201679-16201698 Msa0672090:CDS 50.0%
GTCGTTGCTAAGGATGGTTC+GGG + chr4_4:16201680-16201699 Msa0672090:CDS 50.0%
!! GGTAGCAAAAGTGTTGGAGG+TGG + chr4_4:16201860-16201879 Msa0672090:CDS 50.0%
GGTTGCTGAACGGAAAGTAG+TGG - chr4_4:16201888-16201907 None:intergenic 50.0%
ACTTTCCGTTCAGCAACCGT+TGG + chr4_4:16201890-16201909 Msa0672090:CDS 50.0%
AGTGGGTCCTACATTGGATG+TGG - chr4_4:16203280-16203299 None:intergenic 50.0%
AATTGCTTGTGCTCCACGCT+AGG + chr4_4:16203535-16203554 Msa0672090:intron 50.0%
TTTGCAGCTGCTACTGGTGA+TGG + chr4_4:16203585-16203604 Msa0672090:intron 50.0%
TCGTTCCGGCTCAGATCTAT+CGG + chr4_4:16203674-16203693 Msa0672090:CDS 50.0%
! CTGTGATGGTTTTTGCAGGC+GGG - chr4_4:16203854-16203873 None:intergenic 50.0%
ACCATCACAGTTACCGCACA+AGG + chr4_4:16203864-16203883 Msa0672090:CDS 50.0%
TCCTTGTGCGGTAACTGTGA+TGG - chr4_4:16203868-16203887 None:intergenic 50.0%
! TGGATCGGTTCTTCCTTGTG+CGG - chr4_4:16203880-16203899 None:intergenic 50.0%
! ATTTTTGCCACGGCCTACCA+AGG - chr4_4:16203983-16204002 None:intergenic 50.0%
CAAGCTTCACCTTTCACCGT+TGG + chr4_4:16204188-16204207 Msa0672090:CDS 50.0%
! CTTGTGGGCTACGGCACTTA+GGG - chr4_4:16201319-16201338 None:intergenic 55.0%
! TCTTGTGGGCTACGGCACTT+AGG - chr4_4:16201320-16201339 None:intergenic 55.0%
! TGGTTCGGGCAAGTACACGA+CGG + chr4_4:16201694-16201713 Msa0672090:CDS 55.0%
GATGCTGCACCAAGTGGTAG+TGG + chr4_4:16201731-16201750 Msa0672090:CDS 55.0%
!! CTGCACCAAGTGGTAGTGGA+AGG + chr4_4:16201735-16201754 Msa0672090:CDS 55.0%
TAACAACGGTCCTTCTCGGC+CGG + chr4_4:16202569-16202588 Msa0672090:intron 55.0%
AACCTTAACACCGGCCGAGA+AGG - chr4_4:16202582-16202601 None:intergenic 55.0%
AATCGTACCTTGGTAGGCCG+TGG + chr4_4:16203973-16203992 Msa0672090:CDS 55.0%
! ACCCTGCTGGTTGGTTACCA+TGG + chr4_4:16204048-16204067 Msa0672090:CDS 55.0%
CCCATGGTAACCAACCAGCA+GGG - chr4_4:16204052-16204071 None:intergenic 55.0%
! CCCTGCTGGTTGGTTACCAT+GGG + chr4_4:16204049-16204068 Msa0672090:CDS 55.0%
TCCCATGGTAACCAACCAGC+AGG - chr4_4:16204053-16204072 None:intergenic 55.0%
! GCTGGTTGGTTACCATGGGA+TGG + chr4_4:16204053-16204072 Msa0672090:CDS 55.0%
GGGCAAGTACACGACGGTGA+AGG + chr4_4:16201700-16201719 Msa0672090:CDS 60.0%
!! ACTTGGTGCAGCATCCGTCG+CGG - chr4_4:16201726-16201745 None:intergenic 60.0%
! GTCCTTCTCGGCCGGTGTTA+AGG + chr4_4:16202577-16202596 Msa0672090:intron 60.0%
! ACAGGCGTGCCATTCACCTC+TGG + chr4_4:16204245-16204264 Msa0672090:CDS 60.0%
GCGACGGATGCTGCACCAAG+TGG + chr4_4:16201725-16201744 Msa0672090:CDS 65.0%
CACGACGGTGAAGGCCGCGA+CGG + chr4_4:16201709-16201728 Msa0672090:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 16200961 16204622 16200961 ID=Msa0672090;Name=Msa0672090
chr4_4 mRNA 16200961 16204622 16200961 ID=Msa0672090-mRNA-1;Parent=Msa0672090;Name=Msa0672090-mRNA-1;_AED=0.00;_eAED=0.00;_QI=101|1|1|1|1|1|2|348|510
chr4_4 exon 16200961 16201911 16200961 ID=Msa0672090-mRNA-1:exon:4887;Parent=Msa0672090-mRNA-1
chr4_4 exon 16203592 16204622 16203592 ID=Msa0672090-mRNA-1:exon:4888;Parent=Msa0672090-mRNA-1
chr4_4 five_prime_UTR 16200961 16201061 16200961 ID=Msa0672090-mRNA-1:five_prime_utr;Parent=Msa0672090-mRNA-1
chr4_4 CDS 16201062 16201911 16201062 ID=Msa0672090-mRNA-1:cds;Parent=Msa0672090-mRNA-1
chr4_4 CDS 16203592 16204274 16203592 ID=Msa0672090-mRNA-1:cds;Parent=Msa0672090-mRNA-1
chr4_4 three_prime_UTR 16204275 16204622 16204275 ID=Msa0672090-mRNA-1:three_prime_utr;Parent=Msa0672090-mRNA-1
Gene Sequence

>Msa0672090

ATGGCTTCATTTTTTCCTTTGGTAACAACTCTTATAATATTTCCATTTCTTTTCTCCACTATTGTTTATGGTTACTCATTAAGTTCCATTAAATCATGGTGTAGCCAAACCCCTTATCCTCAACCATGTGAGTATTATTTAACCAATAATGCTTTCAATCAAACCATAAAAAATAAATCCGATTTTTTCAAAGTTTCACTTGAACTTGCTATGGAGAGAGCAAAAAAAGGTGAAGAAAATACACATGCACTTGGCCCTAAGTGCCGTAGCCCACAAGAAAAAGCTGCTTGGGCAGATTGTCTTGAACTCTATGATTTCACAGTCCAAAAGCTTAGCCAAACAAAATACACAAAGTGCACACAATATGAATCCCAAACATGGCTTAGTACAGCTTTAACAAACCTTGAAACATGCAAAAATGGATTTTATGACCTTGGTGTCACAAACTATGTCTTACCTTTGTTGTCTAATAATGTAACAAAGTTGTTAAGTAACACCTTGTCACTCAACAAGGTTCCTTACCAACAACCAAGTTACAAAGATGGATTTCCAACATGGGTTAAACCTGGTGATAGGAAACTTCTTCAAACTTCTTCCGCTGCTTCGAAGGCTAATGTCGTCGTTGCTAAGGATGGTTCGGGCAAGTACACGACGGTGAAGGCCGCGACGGATGCTGCACCAAGTGGTAGTGGAAGGTATGTGATATATGTGAAGGCTGGTGTGTATAATGAACAAGTTGAGATTAAAGCAAAGAATGTCATGTTGGTTGGAGATGGTATTGGTAAAACCATAATCACTGGTAGCAAAAGTGTTGGAGGTGGCACCACTACTTTCCGTTCAGCAACCGTTGCTGCTACTGGTGATGGATTTATTGCTCAAGATATCACATTCAGAAACACAGCTGGAGCTGCAAATCATCAAGCTGTTGCATTTCGTTCCGGCTCAGATCTATCGGTATTTTACAAATGTAGCTTCGAAGGTTATCAAGACACACTTTATGTTCACTCAGAGAGACAATTTTATAGAGAATGTAACATCTATGGGACAGTTGATTTCATCTTTGGAAACGCCGCGGTAGTTTTGCAAAATTGTAACATTTTTGCAAGAAACCCGCCTGCAAAAACCATCACAGTTACCGCACAAGGAAGAACCGATCCAAATCAAAACACTGGAATTATAATTCATAATTCTAGGGTTTCCGCTCAATCGGATTTAAACCCTAGCTCTGTTAAATCGTACCTTGGTAGGCCGTGGCAAAAATATTCAAGAACAGTTTTCATGAAAACTGTTATTGATGGTTTTATTAACCCTGCTGGTTGGTTACCATGGGATGGTAATTTTGCTTTGGATACTTTATATTATGCTGAATATGCTAATACAGGTTCAGGTTCATCCACTTCAAATAGGGTTACATGGAAAGGTTACCATGTTCTTACTAGTGCTTCACAAGCTTCACCTTTCACCGTTGGGAATTTCATTGCCGGTAATTCATGGATAGGAAACACAGGCGTGCCATTCACCTCTGGACTTTAA
Protein sequence

>Msa0672090

MASFFPLVTTLIIFPFLFSTIVYGYSLSSIKSWCSQTPYPQPCEYYLTNNAFNQTIKNKSDFFKVSLELAMERAKKGEENTHALGPKCRSPQEKAAWADCLELYDFTVQKLSQTKYTKCTQYESQTWLSTALTNLETCKNGFYDLGVTNYVLPLLSNNVTKLLSNTLSLNKVPYQQPSYKDGFPTWVKPGDRKLLQTSSAASKANVVVAKDGSGKYTTVKAATDAAPSGSGRYVIYVKAGVYNEQVEIKAKNVMLVGDGIGKTIITGSKSVGGGTTTFRSATVAATGDGFIAQDITFRNTAGAANHQAVAFRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRECNIYGTVDFIFGNAAVVLQNCNIFARNPPAKTITVTAQGRTDPNQNTGIIIHNSRVSAQSDLNPSSVKSYLGRPWQKYSRTVFMKTVIDGFINPAGWLPWDGNFALDTLYYAEYANTGSGSSTSNRVTWKGYHVLTSASQASPFTVGNFIAGNSWIGNTGVPFTSGL*