AlfalfaGEDB | Msa0246300

M. sativa cultivar zhongmu-4 / Msa0246300

KEGG: K21407
GO: GO:0003674 GO:0003824 GO:0004064 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0009986 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0052689
PFAM: PF20067.2

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0246300	XP_003595316.1	91.375	371	32	0	1	371	1	371	0.0	682

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0246300	sp\|Q9SD07\|SSL4_ARATH	31.476	359	224	11	22	368	13	361	6.31e-48	169

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0246300	G7IGG5	91.375	371	32	0	1	371	1	371	0.0	682

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa0246300	0.804276	2.334445e-49	-8.615850e-47
Msa0004350	Msa0246300	0.831284	1.820985e-55	-8.615850e-47
Msa0005160	Msa0246300	0.828102	1.083429e-54	-8.615850e-47
Msa0006700	Msa0246300	0.839035	2.015708e-57	-8.615850e-47
Msa0006820	Msa0246300	0.853877	1.787352e-61	-8.615850e-47
Msa0008850	Msa0246300	0.870700	1.197396e-66	-8.615850e-47
Msa0013810	Msa0246300	0.818125	2.307277e-52	-8.615850e-47
Msa0017120	Msa0246300	0.855184	7.480846e-62	-8.615850e-47
Msa0018870	Msa0246300	0.813040	3.130632e-51	-8.615850e-47
Msa0026930	Msa0246300	0.819311	1.241428e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0246300	MtrunA17_Chr2g0300861	91.375	371	32	0	1	371	1	371	0.0	682
Msa0246300	MtrunA17_Chr2g0300851	84.789	355	51	1	17	371	1	352	0.0	624
Msa0246300	MtrunA17_Chr2g0300841	83.003	353	59	1	20	371	8	360	0.0	602
Msa0246300	MtrunA17_Chr8g0370791	33.043	345	217	10	29	366	27	364	2.16e-52	178
Msa0246300	MtrunA17_Chr8g0370761	32.410	361	214	10	22	366	18	364	1.21e-48	168
Msa0246300	MtrunA17_Chr8g0370771	32.636	239	140	5	136	366	4	229	3.47e-37	134
Msa0246300	MtrunA17_Chr4g0025341	31.200	250	159	4	124	364	142	387	2.40e-33	127
Msa0246300	MtrunA17_Chr2g0329531	27.208	283	194	6	96	367	124	405	5.71e-31	121
Msa0246300	MtrunA17_Chr7g0256601	30.120	249	164	5	126	365	167	414	3.13e-30	120
Msa0246300	MtrunA17_Chr2g0322241	31.600	250	158	8	124	364	146	391	8.49e-29	115

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0246300	AT3G51420.1	31.476	359	224	11	22	368	13	361	6.42e-49	169
Msa0246300	AT3G51430.2	29.581	382	224	11	8	368	2	359	2.20e-47	165
Msa0246300	AT3G51430.1	29.688	384	223	12	8	368	2	361	4.07e-47	164
Msa0246300	AT3G51440.1	32.105	380	219	12	8	368	2	361	6.67e-47	164
Msa0246300	AT3G51450.1	30.081	369	224	12	20	368	7	361	1.79e-42	152
Msa0246300	AT3G57020.2	28.977	352	206	14	43	358	2	345	2.16e-33	127
Msa0246300	AT3G57020.1	31.907	257	151	9	118	358	111	359	1.46e-31	123
Msa0246300	AT1G08470.1	27.348	362	206	12	46	364	38	385	7.12e-31	121
Msa0246300	AT3G57010.1	32.245	245	153	9	124	358	119	360	1.07e-30	120
Msa0246300	AT3G59530.3	25.823	395	239	13	15	370	20	399	1.35e-30	120

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Find 94 sgRNAs with CRISPR-Local

Find 356 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAAGCAAAGAAGTGCT+TGG	0.534736	2_2:-27438508	Msa0246310:three_prime_UTR
AAATGGTGTTGCACTGTCCA+AGG	0.631186	2_2:+27436611	Msa0246300:CDS
AACACACAGCTTCTAAAGCA+TGG	0.587286	2_2:+27432816	Msa0246300:exon
AACTAAAGCTGCTAGAACCA+AGG	0.570761	2_2:-27432899	None:intergenic
AACTTCAGCTGTGGAGTTCG+AGG	0.458627	2_2:+27438650	Msa0246300:CDS
AATAACTGTCTCATTTAACG+TGG	0.678986	2_2:-27436565	Msa0246310:three_prime_UTR
AATATCAATCTTGCTCCTGA+TGG	0.526078	2_2:+27437959	Msa0246300:CDS
AATGGTTAGAAATGGAAATT+GGG	0.411141	2_2:+27433229	Msa0246300:CDS
AATGTGATTAAACTTGGAGA+AGG	0.561564	2_2:+27433125	Msa0246300:CDS
AATTTACCAGGAGTGAAAGG+AGG	0.657241	2_2:-27432856	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAATTGAAAATACAGATGG+TGG	-	chr2_2:27432982-27433001	None:intergenic	30.0%
CAATTACAGGTGAAAACTTA+AGG	+	chr2_2:27433018-27433037	Msa0246300:intron	30.0%
AATGTGATTAAACTTGGAGA+AGG	+	chr2_2:27433125-27433144	Msa0246300:CDS	30.0%
AGATGGTTGGATTAAAAGAA+TGG	+	chr2_2:27433211-27433230	Msa0246300:CDS	30.0%
GAATGGTTAGAAATGGAAAT+TGG	+	chr2_2:27433228-27433247	Msa0246300:CDS	30.0%
AAGCATGGTATATATTCATG+TGG	+	chr2_2:27433456-27433475	Msa0246300:intron	30.0%
AGCATGGTATATATTCATGT+GGG	+	chr2_2:27433457-27433476	Msa0246300:intron	30.0%
TTGTATCTTGGACCTAAAAT+TGG	-	chr2_2:27433518-27433537	None:intergenic	30.0%
ATCAATGCAGAATTGTATCT+TGG	-	chr2_2:27433530-27433549	None:intergenic	30.0%
TACTAAAGTGAAATGTTGTG+AGG	-	chr2_2:27433834-27433853	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	27432716	27438888	27432716	ID=Msa0246300;Name=Msa0246300
chr2_2	mRNA	27432716	27438888	27432716	ID=Msa0246300-mRNA-1;Parent=Msa0246300;Name=Msa0246300-mRNA-1;_AED=0.28;_eAED=0.28;_QI=119\|1\|1\|1\|1\|1\|6\|151\|371
chr2_2	exon	27432716	27433026	27432716	ID=Msa0246300-mRNA-1:exon:9943;Parent=Msa0246300-mRNA-1
chr2_2	exon	27433125	27433337	27433125	ID=Msa0246300-mRNA-1:exon:9944;Parent=Msa0246300-mRNA-1
chr2_2	exon	27433577	27433644	27433577	ID=Msa0246300-mRNA-1:exon:9945;Parent=Msa0246300-mRNA-1
chr2_2	exon	27436431	27436667	27436431	ID=Msa0246300-mRNA-1:exon:9946;Parent=Msa0246300-mRNA-1
chr2_2	exon	27437874	27438006	27437874	ID=Msa0246300-mRNA-1:exon:9947;Parent=Msa0246300-mRNA-1
chr2_2	exon	27438465	27438888	27438465	ID=Msa0246300-mRNA-1:exon:9948;Parent=Msa0246300-mRNA-1
chr2_2	five_prime_UTR	27432716	27432834	27432716	ID=Msa0246300-mRNA-1:five_prime_utr;Parent=Msa0246300-mRNA-1
chr2_2	CDS	27432835	27433026	27432835	ID=Msa0246300-mRNA-1:cds;Parent=Msa0246300-mRNA-1
chr2_2	CDS	27433125	27433337	27433125	ID=Msa0246300-mRNA-1:cds;Parent=Msa0246300-mRNA-1
chr2_2	CDS	27433577	27433644	27433577	ID=Msa0246300-mRNA-1:cds;Parent=Msa0246300-mRNA-1
chr2_2	CDS	27436431	27436667	27436431	ID=Msa0246300-mRNA-1:cds;Parent=Msa0246300-mRNA-1
chr2_2	CDS	27437874	27438006	27437874	ID=Msa0246300-mRNA-1:cds;Parent=Msa0246300-mRNA-1
chr2_2	CDS	27438465	27438737	27438465	ID=Msa0246300-mRNA-1:cds;Parent=Msa0246300-mRNA-1
chr2_2	three_prime_UTR	27438738	27438888	27438738	ID=Msa0246300-mRNA-1:three_prime_utr;Parent=Msa0246300-mRNA-1

Gene Sequence

>Msa0246300

ATGGCTTCCAATATAACCAATCCTCCTTTCACTCCTGGTAAATTTTGTGTCACACTCACCTCAACCTTGGTTCTAGCAGCTTTAGTTTCTCTTACAGTTCAAGTCTTCTACTTCTCACCCATAGATCCAGTTTTGCTAGAAATACCACCATCTGTATTTTCAATTTCTTCAACAAAAAACAACCAATTACAGAATGTGATTAAACTTGGAGAAGGATTTCTGAAGCAACCAGAAGATGTTTGTGTGGATGAAGATGGTGTCTTGTATACAGCTACAAGAGATGGTTGGATTAAAAGAATGGTTAGAAATGGAAATTGGGAGAATTGGAAGCATATAGATAGCTCTTTTTTGCTTGGAATCACTACATCTAAAGATGGTGGTCTCATTGTCTGTGATACTACCTTGGGTTTGCTCAAGGTTACAGAAGAAGGTTTCAGTGTTATCCTTTCACAAGTGAATGGTTCTCAATTAATGTTTGCAGATGATGTAATAGAAGCATCAGATGGAAATATATACTTCAGTGTTGCTAGTAACAAATTTGGTTTACACGATTGGTATTTGGATATGCTAGAGGCAAGACCTCATGGACAACTTTTAAAGTATAATCCCACGTTAAATGAGACAGTTATTGTCATTGATAATTTGACATTTGCAAATGGTGTTGCACTGTCCAAGGATGAAGATTATGTTGTGGTGTGCGAAACTTGGAAATTTAGGTGTGTGAGGCATTGGCTAAAAGGAATTAACAAAGGCAAAACAGATATCTTCATTGAAAACCTCCCTGGTGGACCTGACAATATCAATCTTGCTCCTGATGGCTCATTTTGGATCGCTTTGGTTCAATTAACTTCTAAAAGGCTGGGGTTTGTGCACACCTCTAAAGTATCCAAGCACTTCTTTGCTTCATTTCCAAGGCTATTTAATCTGATCAATAGTGCAACAAAATCAGCTATGGTGGTAAATGTTGGAACTGATGGCAATATTATCAGAAAATTTGGTGATAATGAAGGGAAGGTGTTTAGTTTTGTAACTTCAGCTGTGGAGTTCGAGGACCATCTTTACCTAGGAAGTCTTAATTCTGACTTTGTTGGAAAATTGCCACTGTCAATTGCATAG

Protein sequence

>Msa0246300

MASNITNPPFTPGKFCVTLTSTLVLAALVSLTVQVFYFSPIDPVLLEIPPSVFSISSTKNNQLQNVIKLGEGFLKQPEDVCVDEDGVLYTATRDGWIKRMVRNGNWENWKHIDSSFLLGITTSKDGGLIVCDTTLGLLKVTEEGFSVILSQVNGSQLMFADDVIEASDGNIYFSVASNKFGLHDWYLDMLEARPHGQLLKYNPTLNETVIVIDNLTFANGVALSKDEDYVVVCETWKFRCVRHWLKGINKGKTDIFIENLPGGPDNINLAPDGSFWIALVQLTSKRLGFVHTSKVSKHFFASFPRLFNLINSATKSAMVVNVGTDGNIIRKFGDNEGKVFSFVTSAVEFEDHLYLGSLNSDFVGKLPLSIA*