Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0758820 | XP_003612270.1 | 95.135 | 185 | 9 | 0 | 1 | 185 | 1 | 185 | 5.63e-127 | 365 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0758820 | sp|Q38853|STR15_ARATH | 58.741 | 143 | 55 | 2 | 47 | 185 | 40 | 182 | 2.21e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0758820 | B7FGV4 | 95.135 | 185 | 9 | 0 | 1 | 185 | 1 | 185 | 2.69e-127 | 365 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005160 | Msa0758820 | 0.841067 | 5.947819e-58 | -8.615850e-47 |
| Msa0008850 | Msa0758820 | 0.807442 | 5.045453e-50 | -8.615850e-47 |
| Msa0017120 | Msa0758820 | 0.813510 | 2.468059e-51 | -8.615850e-47 |
| Msa0030740 | Msa0758820 | 0.813742 | 2.194370e-51 | -8.615850e-47 |
| Msa0033590 | Msa0758820 | 0.807423 | 5.092425e-50 | -8.615850e-47 |
| Msa0034340 | Msa0758820 | 0.825078 | 5.702481e-54 | -8.615850e-47 |
| Msa0047470 | Msa0758820 | 0.833700 | 4.587269e-56 | -8.615850e-47 |
| Msa0053210 | Msa0758820 | 0.810961 | 8.882314e-51 | -8.615850e-47 |
| Msa0056040 | Msa0758820 | 0.831658 | 1.473340e-55 | -8.615850e-47 |
| Msa0056130 | Msa0758820 | 0.800987 | 1.113606e-48 | -8.615850e-47 |
| Msa0065390 | Msa0758820 | 0.825877 | 3.688499e-54 | -8.615850e-47 |
| Msa0083160 | Msa0758820 | 0.824175 | 9.307512e-54 | -8.615850e-47 |
| Msa0089210 | Msa0758820 | 0.813647 | 2.302114e-51 | -8.615850e-47 |
| Msa0098550 | Msa0758820 | 0.804659 | 1.942925e-49 | -8.615850e-47 |
| Msa0098700 | Msa0758820 | 0.812186 | 4.812777e-51 | -8.615850e-47 |
| Msa0100250 | Msa0758820 | 0.812867 | 3.415617e-51 | -8.615850e-47 |
| Msa0103060 | Msa0758820 | 0.804843 | 1.778162e-49 | -8.615850e-47 |
| Msa0103780 | Msa0758820 | 0.816119 | 6.516585e-52 | -8.615850e-47 |
| Msa0107460 | Msa0758820 | 0.807743 | 4.355519e-50 | -8.615850e-47 |
| Msa0110710 | Msa0758820 | 0.826704 | 2.343527e-54 | -8.615850e-47 |
| Msa0606060 | Msa0758820 | 0.814361 | 1.602549e-51 | -8.615850e-47 |
| Msa0607280 | Msa0758820 | 0.810411 | 1.168331e-50 | -8.615850e-47 |
| Msa0610280 | Msa0758820 | 0.814964 | 1.177905e-51 | -8.615850e-47 |
| Msa0614230 | Msa0758820 | 0.806820 | 6.830236e-50 | -8.615850e-47 |
| Msa0615850 | Msa0758820 | 0.806937 | 6.454866e-50 | -8.615850e-47 |
| Msa0617120 | Msa0758820 | 0.801708 | 7.924681e-49 | -8.615850e-47 |
| Msa0634420 | Msa0758820 | 0.823830 | 1.121426e-53 | -8.615850e-47 |
| Msa0635990 | Msa0758820 | 0.811305 | 7.484051e-51 | -8.615850e-47 |
| Msa0638710 | Msa0758820 | 0.811394 | 7.157924e-51 | -8.615850e-47 |
| Msa0640880 | Msa0758820 | 0.808550 | 2.930497e-50 | -8.615850e-47 |
| Msa0660820 | Msa0758820 | 0.838716 | 2.437892e-57 | -8.615850e-47 |
| Msa0674320 | Msa0758820 | 0.806284 | 8.863408e-50 | -8.615850e-47 |
| Msa0685520 | Msa0758820 | 0.814707 | 1.342964e-51 | -8.615850e-47 |
| Msa0686610 | Msa0758820 | 0.823867 | 1.099072e-53 | -8.615850e-47 |
| Msa0698900 | Msa0758820 | 0.817904 | 2.588865e-52 | -8.615850e-47 |
| Msa0702070 | Msa0758820 | 0.802592 | 5.214547e-49 | -8.615850e-47 |
| Msa0702120 | Msa0758820 | 0.800005 | 1.765184e-48 | -8.615850e-47 |
| Msa0705050 | Msa0758820 | 0.807573 | 4.732854e-50 | -8.615850e-47 |
| Msa0719340 | Msa0758820 | 0.807127 | 5.882244e-50 | -8.615850e-47 |
| Msa0729590 | Msa0758820 | 0.812031 | 5.201329e-51 | -8.615850e-47 |
| Msa0734000 | Msa0758820 | 0.805387 | 1.367848e-49 | -8.615850e-47 |
| Msa0738870 | Msa0758820 | 0.823838 | 1.116179e-53 | -8.615850e-47 |
| Msa0738880 | Msa0758820 | 0.803624 | 3.190359e-49 | -8.615850e-47 |
| Msa0758790 | Msa0758820 | 0.821922 | 3.119992e-53 | -8.615850e-47 |
| Msa0758820 | Msa0763910 | 0.800683 | 1.284412e-48 | -8.615850e-47 |
| Msa0758820 | Msa0766880 | 0.823410 | 1.406199e-53 | -8.615850e-47 |
| Msa0758820 | Msa0771960 | 0.810122 | 1.348690e-50 | -8.615850e-47 |
| Msa0758820 | Msa0772150 | 0.800468 | 1.421383e-48 | -8.615850e-47 |
| Msa0758820 | Msa0772210 | 0.804222 | 2.395612e-49 | -8.615850e-47 |
| Msa0758820 | Msa0794130 | 0.812884 | 3.386842e-51 | -8.615850e-47 |
| Msa0758820 | Msa0819320 | 0.801669 | 8.073814e-49 | -8.615850e-47 |
| Msa0758820 | Msa0819330 | 0.822259 | 2.606276e-53 | -8.615850e-47 |
| Msa0758820 | Msa0819360 | 0.802615 | 5.156968e-49 | -8.615850e-47 |
| Msa0758820 | Msa0819370 | 0.805654 | 1.202373e-49 | -8.615850e-47 |
| Msa0758820 | Msa0822370 | 0.806208 | 9.198859e-50 | -8.615850e-47 |
| Msa0758820 | Msa0838630 | 0.935101 | 1.437913e-96 | -8.615850e-47 |
| Msa0758820 | Msa0838660 | 0.950224 | 2.558333e-108 | -8.615850e-47 |
| Msa0758820 | Msa0859520 | 0.812138 | 4.930437e-51 | -8.615850e-47 |
| Msa0758820 | Msa0930160 | 0.830788 | 2.410637e-55 | -8.615850e-47 |
| Msa0758820 | Msa0934830 | 0.832025 | 1.196090e-55 | -8.615850e-47 |
| Msa0758820 | Msa0955310 | 0.802586 | 5.228468e-49 | -8.615850e-47 |
| Msa0758820 | Msa0967670 | 0.804176 | 2.449550e-49 | -8.615850e-47 |
| Msa0758820 | Msa0971860 | 0.832384 | 9.749088e-56 | -8.615850e-47 |
| Msa0758820 | Msa1030430 | 0.805346 | 1.395557e-49 | -8.615850e-47 |
| Msa0758820 | Msa1030870 | 0.820224 | 7.679477e-53 | -8.615850e-47 |
| Msa0758820 | Msa1033160 | 0.807281 | 5.455920e-50 | -8.615850e-47 |
| Msa0758820 | Msa1043870 | 0.800874 | 1.174464e-48 | -8.615850e-47 |
| Msa0758820 | Msa1102180 | 0.819817 | 9.517802e-53 | -8.615850e-47 |
| Msa0758820 | Msa1115580 | 0.827781 | 1.294120e-54 | -8.615850e-47 |
| Msa0758820 | Msa1124440 | 0.801377 | 9.264594e-49 | -8.615850e-47 |
| Msa0758820 | Msa1132540 | 0.808301 | 3.312739e-50 | -8.615850e-47 |
| Msa0758820 | Msa1157730 | 0.800336 | 1.511578e-48 | -8.615850e-47 |
| Msa0758820 | Msa1166180 | 0.800782 | 1.226474e-48 | -8.615850e-47 |
| Msa0758820 | Msa1173410 | 0.803326 | 3.677781e-49 | -8.615850e-47 |
| Msa0758820 | Msa1182990 | 0.801938 | 7.110325e-49 | -8.615850e-47 |
| Msa0758820 | Msa1190820 | 0.814386 | 1.581730e-51 | -8.615850e-47 |
| Msa0758820 | Msa1198450 | 0.852150 | 5.575867e-61 | -8.615850e-47 |
| Msa0758820 | Msa1219250 | 0.800809 | 1.210517e-48 | -8.615850e-47 |
| Msa0758820 | Msa1220740 | 0.864260 | 1.382003e-64 | -8.615850e-47 |
| Msa0758820 | Msa1302780 | 0.827914 | 1.202279e-54 | -8.615850e-47 |
| Msa0758820 | Msa1310300 | 0.803775 | 2.967188e-49 | -8.615850e-47 |
| Msa0758820 | Msa1334650 | 0.856196 | 3.789486e-62 | -8.615850e-47 |
| Msa0758820 | Msa1365210 | 0.834551 | 2.807295e-56 | -8.615850e-47 |
| Msa0758820 | Msa1372560 | 0.808465 | 3.056212e-50 | -8.615850e-47 |
| Msa0758820 | Msa1388850 | 0.832849 | 7.475520e-56 | -8.615850e-47 |
| Msa0758820 | Msa1392680 | 0.837084 | 6.402655e-57 | -8.615850e-47 |
| Msa0758820 | Msa1405770 | 0.812769 | 3.588944e-51 | -8.615850e-47 |
| Msa0758820 | Msa1411830 | 0.823194 | 1.579647e-53 | -8.615850e-47 |
| Msa0758820 | Msa1423190 | 0.806059 | 9.886829e-50 | -8.615850e-47 |
| Msa0758820 | Msa1440180 | 0.833196 | 6.126552e-56 | -8.615850e-47 |
| Msa0758820 | Msa1449740 | 0.831535 | 1.579899e-55 | -8.615850e-47 |
| Msa0758820 | Msa1458920 | 0.800687 | 1.282134e-48 | -8.615850e-47 |
| Msa0141670 | Msa0758820 | 0.804157 | 2.471520e-49 | -8.615850e-47 |
| Msa0146600 | Msa0758820 | 0.820473 | 6.732547e-53 | -8.615850e-47 |
| Msa0150210 | Msa0758820 | 0.823780 | 1.151878e-53 | -8.615850e-47 |
| Msa0157020 | Msa0758820 | 0.822477 | 2.319690e-53 | -8.615850e-47 |
| Msa0178940 | Msa0758820 | 0.806680 | 7.314744e-50 | -8.615850e-47 |
| Msa0203180 | Msa0758820 | 0.835944 | 1.248985e-56 | -8.615850e-47 |
| Msa0369780 | Msa0758820 | 0.859498 | 3.968521e-63 | -8.615850e-47 |
| Msa0375430 | Msa0758820 | 0.803275 | 3.766963e-49 | -8.615850e-47 |
| Msa0376480 | Msa0758820 | 0.806084 | 9.767127e-50 | -8.615850e-47 |
| Msa0377460 | Msa0758820 | 0.805306 | 1.422957e-49 | -8.615850e-47 |
| Msa0387790 | Msa0758820 | 0.811816 | 5.794411e-51 | -8.615850e-47 |
| Msa0393540 | Msa0758820 | 0.801000 | 1.106590e-48 | -8.615850e-47 |
| Msa0417150 | Msa0758820 | 0.842213 | 2.966832e-58 | -8.615850e-47 |
| Msa0420800 | Msa0758820 | 0.808274 | 3.356752e-50 | -8.615850e-47 |
| Msa0421800 | Msa0758820 | 0.834242 | 3.357354e-56 | -8.615850e-47 |
| Msa0425080 | Msa0758820 | 0.803178 | 3.945403e-49 | -8.615850e-47 |
| Msa0426860 | Msa0758820 | 0.822569 | 2.208161e-53 | -8.615850e-47 |
| Msa0426890 | Msa0758820 | 0.805132 | 1.547144e-49 | -8.615850e-47 |
| Msa0427050 | Msa0758820 | 0.815673 | 8.193458e-52 | -8.615850e-47 |
| Msa0463990 | Msa0758820 | 0.846816 | 1.712381e-59 | -8.615850e-47 |
| Msa0467080 | Msa0758820 | 0.810691 | 1.016437e-50 | -8.615850e-47 |
| Msa0467800 | Msa0758820 | 0.835020 | 2.139960e-56 | -8.615850e-47 |
| Msa0467880 | Msa0758820 | 0.841177 | 5.565468e-58 | -8.615850e-47 |
| Msa0472270 | Msa0758820 | 0.836000 | 1.209237e-56 | -8.615850e-47 |
| Msa0473320 | Msa0758820 | 0.802282 | 6.041925e-49 | -8.615850e-47 |
| Msa0473450 | Msa0758820 | 0.800323 | 1.520931e-48 | -8.615850e-47 |
| Msa0246300 | Msa0758820 | 0.839214 | 1.811251e-57 | -8.615850e-47 |
| Msa0250520 | Msa0758820 | 0.810949 | 8.936328e-51 | -8.615850e-47 |
| Msa0270460 | Msa0758820 | 0.807687 | 4.474922e-50 | -8.615850e-47 |
| Msa0284260 | Msa0758820 | 0.820805 | 5.648771e-53 | -8.615850e-47 |
| Msa0284870 | Msa0758820 | 0.838502 | 2.768746e-57 | -8.615850e-47 |
| Msa0292860 | Msa0758820 | 0.838658 | 2.523178e-57 | -8.615850e-47 |
| Msa0323630 | Msa0758820 | 0.815474 | 9.073310e-52 | -8.615850e-47 |
| Msa0349000 | Msa0758820 | 0.803869 | 2.837074e-49 | -8.615850e-47 |
| Msa0484220 | Msa0758820 | 0.885015 | 1.169617e-71 | -8.615850e-47 |
| Msa0485280 | Msa0758820 | 0.804331 | 2.274211e-49 | -8.615850e-47 |
| Msa0509540 | Msa0758820 | 0.859389 | 4.278071e-63 | -8.615850e-47 |
| Msa0512310 | Msa0758820 | 0.804899 | 1.731049e-49 | -8.615850e-47 |
| Msa0514260 | Msa0758820 | 0.830313 | 3.150123e-55 | -8.615850e-47 |
| Msa0517400 | Msa0758820 | 0.807520 | 4.856802e-50 | -8.615850e-47 |
| Msa0534420 | Msa0758820 | 0.820678 | 6.042464e-53 | -8.615850e-47 |
| Msa0538000 | Msa0758820 | 0.804428 | 2.170793e-49 | -8.615850e-47 |
| Msa0538260 | Msa0758820 | 0.816742 | 4.727000e-52 | -8.615850e-47 |
| Msa0550630 | Msa0758820 | 0.830561 | 2.739959e-55 | -8.615850e-47 |
| Msa0577870 | Msa0758820 | 0.802820 | 4.679069e-49 | -8.615850e-47 |
| Msa0598250 | Msa0758820 | 0.823910 | 1.073878e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0758820 | MtrunA17_Chr5g0406281 | 95.135 | 185 | 9 | 0 | 1 | 185 | 1 | 185 | 5.18e-131 | 365 |
| Msa0758820 | MtrunA17_Chr5g0405631 | 49.123 | 114 | 56 | 1 | 74 | 185 | 31 | 144 | 1.39e-31 | 111 |
| Msa0758820 | MtrunA17_Chr2g0284621 | 42.857 | 112 | 63 | 1 | 75 | 185 | 12 | 123 | 4.74e-29 | 104 |
| Msa0758820 | MtrunA17_Chr8g0371681 | 45.833 | 120 | 59 | 2 | 61 | 178 | 5 | 120 | 7.09e-28 | 101 |
| Msa0758820 | MtrunA17_Chr4g0020691 | 39.200 | 125 | 71 | 2 | 62 | 185 | 3 | 123 | 7.52e-28 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0758820 | AT4G35770.1 | 58.741 | 143 | 55 | 2 | 47 | 185 | 40 | 182 | 2.25e-56 | 176 |
| Msa0758820 | AT5G66040.1 | 65.487 | 113 | 39 | 0 | 72 | 184 | 7 | 119 | 5.14e-51 | 160 |
| Msa0758820 | AT4G35770.2 | 57.143 | 119 | 47 | 2 | 47 | 161 | 40 | 158 | 4.30e-42 | 139 |
| Msa0758820 | AT4G35770.3 | 57.143 | 119 | 47 | 2 | 47 | 161 | 40 | 158 | 6.40e-42 | 139 |
| Msa0758820 | AT4G35770.5 | 56.436 | 101 | 40 | 2 | 47 | 143 | 40 | 140 | 1.80e-33 | 117 |
| Msa0758820 | AT5G66170.3 | 49.107 | 112 | 51 | 2 | 67 | 176 | 12 | 119 | 9.46e-31 | 109 |
| Msa0758820 | AT5G66170.2 | 48.246 | 114 | 51 | 3 | 67 | 176 | 12 | 121 | 8.62e-29 | 104 |
| Msa0758820 | AT2G21045.1 | 41.441 | 111 | 65 | 0 | 75 | 185 | 43 | 153 | 5.87e-27 | 100 |
| Msa0758820 | AT5G66170.1 | 50.515 | 97 | 42 | 2 | 67 | 161 | 12 | 104 | 5.19e-24 | 92.0 |
| Msa0758820 | AT5G66040.2 | 60.938 | 64 | 25 | 0 | 121 | 184 | 1 | 64 | 6.24e-23 | 87.4 |
| Msa0758820 | AT2G17850.1 | 40.385 | 104 | 60 | 1 | 75 | 176 | 34 | 137 | 1.05e-21 | 86.7 |
| Msa0758820 | AT4G35770.4 | 54.430 | 79 | 32 | 2 | 47 | 121 | 40 | 118 | 2.97e-21 | 84.7 |
| Msa0758820 | AT4G35770.6 | 54.430 | 79 | 32 | 2 | 47 | 121 | 40 | 118 | 2.97e-21 | 84.7 |
| Msa0758820 | AT2G17850.3 | 33.929 | 112 | 63 | 2 | 77 | 177 | 59 | 170 | 4.44e-16 | 72.8 |
| Msa0758820 | AT2G17850.2 | 39.326 | 89 | 52 | 1 | 75 | 161 | 34 | 122 | 2.72e-15 | 69.3 |
Find 62 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTATGTCCCTTATTAAATT+TGG | 0.146508 | 5_2:-14195618 | None:intergenic |
| AAAATCTTCCAAATTTAATA+AGG | 0.178231 | 5_2:+14195610 | Msa0758820:CDS |
| TCTAGCTGGCCATAAGTATT+TGG | 0.181385 | 5_2:+14195858 | Msa0758820:CDS |
| CTTTCATTAAATAATGTATT+TGG | 0.190814 | 5_2:-14195169 | None:intergenic |
| TACATAAGAGGTACTATAAT+TGG | 0.198075 | 5_2:+14195243 | Msa0758820:five_prime_UTR |
| ACCTTACATCCAAATACTTA+TGG | 0.228445 | 5_2:-14195867 | None:intergenic |
| GTGTGGTTTAAAGGGGAATT+TGG | 0.241136 | 5_2:+14195780 | Msa0758820:CDS |
| AAGCACGATGTGTGGTTTAA+AGG | 0.244947 | 5_2:+14195771 | Msa0758820:intron |
| AGCATATCATGATTTCTTCT+TGG | 0.301007 | 5_2:-14197415 | None:intergenic |
| GATCATGTAAATGCATAAAA+AGG | 0.312692 | 5_2:+14197196 | Msa0758820:three_prime_UTR |
| TTATTTCTATATACAGCTTC+AGG | 0.321929 | 5_2:-14197158 | None:intergenic |
| AGCACGATGTGTGGTTTAAA+GGG | 0.328888 | 5_2:+14195772 | Msa0758820:intron |
| GATCCAACTTTGTACATATA+AGG | 0.335693 | 5_2:-14196223 | None:intergenic |
| TATGCTGCTTGGACTCAAAA+TGG | 0.348576 | 5_2:+14197111 | Msa0758820:CDS |
| ATGAAGTATCATGTAATTCT+TGG | 0.358857 | 5_2:+14197601 | Msa0758820:three_prime_UTR |
| AAATCTTCCAAATTTAATAA+GGG | 0.364373 | 5_2:+14195611 | Msa0758820:CDS |
| TTAAATGATTGTTACATAAG+AGG | 0.390886 | 5_2:+14195231 | Msa0758820:five_prime_UTR |
| ATTAGAGTGTGACCTTGTTA+TGG | 0.398979 | 5_2:-14195448 | None:intergenic |
| GGGAATTTGGAGGAAGTTGT+TGG | 0.404121 | 5_2:+14195793 | Msa0758820:CDS |
| GCCATAAGTATTTGGATGTA+AGG | 0.427420 | 5_2:+14195866 | Msa0758820:CDS |
| GAAATAATAAGAATTCTGTT+TGG | 0.432163 | 5_2:+14197174 | Msa0758820:three_prime_UTR |
| TAGCTGGTGGTTATGCTGCT+TGG | 0.436274 | 5_2:+14197100 | Msa0758820:CDS |
| ACACGCACTGGCACTGAAGT+TGG | 0.437130 | 5_2:-14195820 | None:intergenic |
| ACCAGCTATGTCTGTGAGTC+CGG | 0.440393 | 5_2:-14197085 | None:intergenic |
| TCTGAAGAAACCCTCTCTCT+TGG | 0.444875 | 5_2:+14197291 | Msa0758820:three_prime_UTR |
| CCTTCATATTATTACTGTTG+TGG | 0.444925 | 5_2:-14197528 | None:intergenic |
| TACATATCAATAATTAATGA+AGG | 0.448491 | 5_2:+14197248 | Msa0758820:three_prime_UTR |
| AACCAAAGTGTTCCAACTTC+CGG | 0.448542 | 5_2:+14195654 | Msa0758820:CDS |
| GTGTTCTGCCGTAGAATTAG+AGG | 0.450733 | 5_2:-14195423 | None:intergenic |
| TGGAAAGAGATTAAAATCTT+CGG | 0.455096 | 5_2:+14195263 | Msa0758820:five_prime_UTR |
| TTCGAATTGAACTGTAAAGA+TGG | 0.461998 | 5_2:-14197340 | None:intergenic |
| TATATGTACAAAGTTGGATC+AGG | 0.472445 | 5_2:+14196226 | Msa0758820:CDS |
| TTTAATGCTGGACACGCTCC+TGG | 0.480736 | 5_2:+14196187 | Msa0758820:CDS |
| ACTTGCTACAGAACAATGAT+TGG | 0.496501 | 5_2:+14197134 | Msa0758820:exon |
| ATACCTTATATGTACAAAGT+TGG | 0.502957 | 5_2:+14196220 | Msa0758820:CDS |
| TGCATTGTATAGGGGTTCAC+CGG | 0.511879 | 5_2:+14197066 | Msa0758820:intron |
| GAAATTATGAAGAAATTGAA+AGG | 0.514237 | 5_2:-14197506 | None:intergenic |
| GCTCACGAGCTTCTTCTAGC+TGG | 0.514761 | 5_2:+14195844 | Msa0758820:CDS |
| AGGGTTGTCAGCTTGGGAAA+AGG | 0.515357 | 5_2:+14196661 | Msa0758820:intron |
| AGCTCGTGAGCTACACGCAC+TGG | 0.517304 | 5_2:-14195832 | None:intergenic |
| TTCGAACCATGATCATTGAC+AGG | 0.518961 | 5_2:+14197357 | Msa0758820:three_prime_UTR |
| CCACAACAGTAATAATATGA+AGG | 0.529671 | 5_2:+14197528 | Msa0758820:three_prime_UTR |
| TACCGGAAGTTGGAACACTT+TGG | 0.532325 | 5_2:-14195656 | None:intergenic |
| ATTCACGTCCTCTAATTCTA+CGG | 0.536092 | 5_2:+14195415 | Msa0758820:CDS |
| ACCACGGAAGAGTTTAATGC+TGG | 0.548422 | 5_2:+14196175 | Msa0758820:CDS |
| TGGTTTAAAGGGGAATTTGG+AGG | 0.552343 | 5_2:+14195783 | Msa0758820:CDS |
| GCTTGGGAAAAGGTCTATGA+TGG | 0.555495 | 5_2:+14196671 | Msa0758820:CDS |
| AAATTATGAAGAAATTGAAA+GGG | 0.566952 | 5_2:-14197505 | None:intergenic |
| ACCGGACTCACAGACATAGC+TGG | 0.570324 | 5_2:+14197084 | Msa0758820:CDS |
| TAAGGTATGTTAATCGCACC+AGG | 0.610995 | 5_2:-14196205 | None:intergenic |
| GCAAGCGGCGGAGACATCAA+AGG | 0.618010 | 5_2:-14195388 | None:intergenic |
| GCACGATGTGTGGTTTAAAG+GGG | 0.620601 | 5_2:+14195773 | Msa0758820:intron |
| ATTGTGATTGTGCAGGACCA+CGG | 0.621639 | 5_2:+14196159 | Msa0758820:intron |
| TCCAGCATTAAACTCTTCCG+TGG | 0.640037 | 5_2:-14196176 | None:intergenic |
| ACACATCAAGCCAAGAGAGA+GGG | 0.640638 | 5_2:-14197301 | None:intergenic |
| GGCAGAACACGACCATAACA+AGG | 0.641310 | 5_2:+14195436 | Msa0758820:CDS |
| AACACATCAAGCCAAGAGAG+AGG | 0.646052 | 5_2:-14197302 | None:intergenic |
| AAATACTATGAAATGCAAAG+TGG | 0.646380 | 5_2:-14195327 | None:intergenic |
| TCGAATTGAACTGTAAAGAT+GGG | 0.648236 | 5_2:-14197339 | None:intergenic |
| AGGACGTGAATGAAAGCAAG+CGG | 0.673997 | 5_2:-14195403 | None:intergenic |
| ACGTGAATGAAAGCAAGCGG+CGG | 0.698419 | 5_2:-14195400 | None:intergenic |
| GGACTCACAGACATAGCTGG+TGG | 0.717723 | 5_2:+14197087 | Msa0758820:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTTCATTAAATAATGTATT+TGG | - | chr5_2:14195172-14195191 | None:intergenic | 15.0% |
| !! | AAAATCTTCCAAATTTAATA+AGG | + | chr5_2:14195610-14195629 | Msa0758820:CDS | 15.0% |
| !! | AAATCTTCCAAATTTAATAA+GGG | + | chr5_2:14195611-14195630 | Msa0758820:CDS | 15.0% |
| !! | CAAAACATTAGTATATTAAT+CGG | - | chr5_2:14196090-14196109 | None:intergenic | 15.0% |
| !! | ATATGAATAGATTAGAAAAA+AGG | - | chr5_2:14196439-14196458 | None:intergenic | 15.0% |
| !! | TACATATCAATAATTAATGA+AGG | + | chr5_2:14197248-14197267 | Msa0758820:three_prime_UTR | 15.0% |
| !! | AAATTATGAAGAAATTGAAA+GGG | - | chr5_2:14197508-14197527 | None:intergenic | 15.0% |
| !! | TTAAATGATTGTTACATAAG+AGG | + | chr5_2:14195231-14195250 | Msa0758820:five_prime_UTR | 20.0% |
| !! | TATATAGAGATATAGAAGAA+TGG | - | chr5_2:14195478-14195497 | None:intergenic | 20.0% |
| !! | TAAAGTAACCAATTTAATCA+AGG | + | chr5_2:14195889-14195908 | Msa0758820:intron | 20.0% |
| !! | TATTTAAGCCTTGATTAAAT+TGG | - | chr5_2:14195900-14195919 | None:intergenic | 20.0% |
| !!! | CATAATAGTTTTGTAGTTTT+AGG | - | chr5_2:14195963-14195982 | None:intergenic | 20.0% |
| !!! | CGATTAATATACTAATGTTT+TGG | + | chr5_2:14196088-14196107 | Msa0758820:intron | 20.0% |
| !! | TTGTAATATTCTTAGTGTTT+AGG | + | chr5_2:14196277-14196296 | Msa0758820:intron | 20.0% |
| !! | TAAGAGATATATAATACTCT+TGG | + | chr5_2:14196328-14196347 | Msa0758820:intron | 20.0% |
| !!! | AGTTGTTTTGATTAATCTAA+TGG | - | chr5_2:14196354-14196373 | None:intergenic | 20.0% |
| !! | AAATAACATACAAAATCTCA+TGG | - | chr5_2:14196596-14196615 | None:intergenic | 20.0% |
| !!! | TTATTTTTGTTTACACTTTC+AGG | - | chr5_2:14196924-14196943 | None:intergenic | 20.0% |
| !!! | TTTTCATCAATAATTAGTCT+TGG | + | chr5_2:14196956-14196975 | Msa0758820:intron | 20.0% |
| !!! | TTAACTTCTTGAAAATTTTG+AGG | + | chr5_2:14197016-14197035 | Msa0758820:intron | 20.0% |
| !! | GAAATAATAAGAATTCTGTT+TGG | + | chr5_2:14197174-14197193 | Msa0758820:three_prime_UTR | 20.0% |
| !! | GAAATTATGAAGAAATTGAA+AGG | - | chr5_2:14197509-14197528 | None:intergenic | 20.0% |
| !!! | TACATAAGAGGTACTATAAT+TGG | + | chr5_2:14195243-14195262 | Msa0758820:five_prime_UTR | 25.0% |
| ! | TGGAAAGAGATTAAAATCTT+CGG | + | chr5_2:14195263-14195282 | Msa0758820:five_prime_UTR | 25.0% |
| !!! | CTTTTTTATGAAATGCTTTG+AGG | - | chr5_2:14195303-14195322 | None:intergenic | 25.0% |
| ! | AAATACTATGAAATGCAAAG+TGG | - | chr5_2:14195330-14195349 | None:intergenic | 25.0% |
| ! | TCTATGTCCCTTATTAAATT+TGG | - | chr5_2:14195621-14195640 | None:intergenic | 25.0% |
| !!! | AATGTTTTGGAATTGTGATT+TGG | + | chr5_2:14196101-14196120 | Msa0758820:intron | 25.0% |
| ! | ATACCTTATATGTACAAAGT+TGG | + | chr5_2:14196220-14196239 | Msa0758820:CDS | 25.0% |
| ! | TAGATTAATCAAAACAACTG+AGG | + | chr5_2:14196355-14196374 | Msa0758820:intron | 25.0% |
| ! | AGATCAAAAAGAGATCAAAT+TGG | + | chr5_2:14196399-14196418 | Msa0758820:intron | 25.0% |
| ! | ATTAGAAAAAAGGACACTAA+TGG | - | chr5_2:14196429-14196448 | None:intergenic | 25.0% |
| ! | CATTAGCAGATGAATTAATT+TGG | - | chr5_2:14196628-14196647 | None:intergenic | 25.0% |
| ! | TATTGATGAAAAAAGTAGCA+CGG | - | chr5_2:14196948-14196967 | None:intergenic | 25.0% |
| !! | GTTAATAAAGTATCTATCAC+TGG | - | chr5_2:14197001-14197020 | None:intergenic | 25.0% |
| !!! | ACAATTGTTTTGCATTGTAT+AGG | + | chr5_2:14197056-14197075 | Msa0758820:intron | 25.0% |
| !!! | CAATTGTTTTGCATTGTATA+GGG | + | chr5_2:14197057-14197076 | Msa0758820:intron | 25.0% |
| !!! | AATTGTTTTGCATTGTATAG+GGG | + | chr5_2:14197058-14197077 | Msa0758820:intron | 25.0% |
| !!! | TTATTTCTATATACAGCTTC+AGG | - | chr5_2:14197161-14197180 | None:intergenic | 25.0% |
| ! | GATCATGTAAATGCATAAAA+AGG | + | chr5_2:14197196-14197215 | Msa0758820:three_prime_UTR | 25.0% |
| ! | ATGTAAATGCATAAAAAGGA+AGG | + | chr5_2:14197200-14197219 | Msa0758820:three_prime_UTR | 25.0% |
| !! | ATGAAGTATCATGTAATTCT+TGG | + | chr5_2:14197601-14197620 | Msa0758820:three_prime_UTR | 25.0% |
| !! | TCAACACTTTTTACACTTAC+CGG | - | chr5_2:14195676-14195695 | None:intergenic | 30.0% |
| ACCTTACATCCAAATACTTA+TGG | - | chr5_2:14195870-14195889 | None:intergenic | 30.0% | |
| ACAATCACAATAAACAGGTA+TGG | - | chr5_2:14196150-14196169 | None:intergenic | 30.0% | |
| GATCCAACTTTGTACATATA+AGG | - | chr5_2:14196226-14196245 | None:intergenic | 30.0% | |
| TATATGTACAAAGTTGGATC+AGG | + | chr5_2:14196226-14196245 | Msa0758820:CDS | 30.0% | |
| AATTATGTGTTCATGCATAC+AGG | + | chr5_2:14196477-14196496 | Msa0758820:intron | 30.0% | |
| ATTATGTGTTCATGCATACA+GGG | + | chr5_2:14196478-14196497 | Msa0758820:intron | 30.0% | |
| ! | GAACAGCAATTTTGTTAAAG+AGG | + | chr5_2:14196509-14196528 | Msa0758820:CDS | 30.0% |
| ! | ATCTTCTTTTCTAAACTGTG+AGG | - | chr5_2:14196538-14196557 | None:intergenic | 30.0% |
| !! | CTAATGTTATTGCTTTTGCA+GGG | + | chr5_2:14196642-14196661 | Msa0758820:intron | 30.0% |
| AACTCAAACAAGAAGTGAAA+GGG | - | chr5_2:14196862-14196881 | None:intergenic | 30.0% | |
| AAACTCAAACAAGAAGTGAA+AGG | - | chr5_2:14196863-14196882 | None:intergenic | 30.0% | |
| AAGTATCTATCACTGGAAAT+TGG | - | chr5_2:14196994-14197013 | None:intergenic | 30.0% | |
| TCGAATTGAACTGTAAAGAT+GGG | - | chr5_2:14197342-14197361 | None:intergenic | 30.0% | |
| TTCGAATTGAACTGTAAAGA+TGG | - | chr5_2:14197343-14197362 | None:intergenic | 30.0% | |
| !!! | TTTTTTCCTGTCAATGATCA+TGG | - | chr5_2:14197366-14197385 | None:intergenic | 30.0% |
| AGCATATCATGATTTCTTCT+TGG | - | chr5_2:14197418-14197437 | None:intergenic | 30.0% | |
| CCTTCATATTATTACTGTTG+TGG | - | chr5_2:14197531-14197550 | None:intergenic | 30.0% | |
| CCACAACAGTAATAATATGA+AGG | + | chr5_2:14197528-14197547 | Msa0758820:three_prime_UTR | 30.0% | |
| ATTCACGTCCTCTAATTCTA+CGG | + | chr5_2:14195415-14195434 | Msa0758820:CDS | 35.0% | |
| ATTAGAGTGTGACCTTGTTA+TGG | - | chr5_2:14195451-14195470 | None:intergenic | 35.0% | |
| ! | TTTTTACACTTACCGGAAGT+TGG | - | chr5_2:14195669-14195688 | None:intergenic | 35.0% |
| !!! | TTTTTTCAAAGCACGATGTG+TGG | + | chr5_2:14195763-14195782 | Msa0758820:intron | 35.0% |
| GCCATAAGTATTTGGATGTA+AGG | + | chr5_2:14195866-14195885 | Msa0758820:CDS | 35.0% | |
| CAACTGAGGTTTGATAGAAT+AGG | + | chr5_2:14196369-14196388 | Msa0758820:intron | 35.0% | |
| ACATACAAAATCTCATGGTG+CGG | - | chr5_2:14196591-14196610 | None:intergenic | 35.0% | |
| !! | GCTAATGTTATTGCTTTTGC+AGG | + | chr5_2:14196641-14196660 | Msa0758820:intron | 35.0% |
| ACTCAAACAAGAAGTGAAAG+GGG | - | chr5_2:14196861-14196880 | None:intergenic | 35.0% | |
| TAGCACTCAACTAAAATGCT+AGG | - | chr5_2:14196889-14196908 | None:intergenic | 35.0% | |
| !!! | TAGCATTTTAGTTGAGTGCT+AGG | + | chr5_2:14196888-14196907 | Msa0758820:intron | 35.0% |
| !!! | TTTTGAGGTCAAAGCTTGTT+TGG | + | chr5_2:14197031-14197050 | Msa0758820:intron | 35.0% |
| ! | ACTTGCTACAGAACAATGAT+TGG | + | chr5_2:14197134-14197153 | Msa0758820:exon | 35.0% |
| AATGCATAAAAAGGAAGGAG+TGG | + | chr5_2:14197205-14197224 | Msa0758820:three_prime_UTR | 35.0% | |
| ! | AACCAAAGTGTTCCAACTTC+CGG | + | chr5_2:14195654-14195673 | Msa0758820:CDS | 40.0% |
| AAGCACGATGTGTGGTTTAA+AGG | + | chr5_2:14195771-14195790 | Msa0758820:intron | 40.0% | |
| AGCACGATGTGTGGTTTAAA+GGG | + | chr5_2:14195772-14195791 | Msa0758820:intron | 40.0% | |
| GTGTGGTTTAAAGGGGAATT+TGG | + | chr5_2:14195780-14195799 | Msa0758820:CDS | 40.0% | |
| TGGTTTAAAGGGGAATTTGG+AGG | + | chr5_2:14195783-14195802 | Msa0758820:CDS | 40.0% | |
| TCTAGCTGGCCATAAGTATT+TGG | + | chr5_2:14195858-14195877 | Msa0758820:CDS | 40.0% | |
| ! | GTATGGAAAGTCGTGAGTTT+TGG | - | chr5_2:14196133-14196152 | None:intergenic | 40.0% |
| CCTGCACAATCACAATAAAC+AGG | - | chr5_2:14196155-14196174 | None:intergenic | 40.0% | |
| CCTGTTTATTGTGATTGTGC+AGG | + | chr5_2:14196152-14196171 | Msa0758820:intron | 40.0% | |
| TAAGGTATGTTAATCGCACC+AGG | - | chr5_2:14196208-14196227 | None:intergenic | 40.0% | |
| CTCAAACAAGAAGTGAAAGG+GGG | - | chr5_2:14196860-14196879 | None:intergenic | 40.0% | |
| ! | TATGCTGCTTGGACTCAAAA+TGG | + | chr5_2:14197111-14197130 | Msa0758820:CDS | 40.0% |
| CATAAAAAGGAAGGAGTGGA+TGG | + | chr5_2:14197209-14197228 | Msa0758820:three_prime_UTR | 40.0% | |
| TTCGAACCATGATCATTGAC+AGG | + | chr5_2:14197357-14197376 | Msa0758820:three_prime_UTR | 40.0% | |
| AGGACGTGAATGAAAGCAAG+CGG | - | chr5_2:14195406-14195425 | None:intergenic | 45.0% | |
| GTGTTCTGCCGTAGAATTAG+AGG | - | chr5_2:14195426-14195445 | None:intergenic | 45.0% | |
| TACCGGAAGTTGGAACACTT+TGG | - | chr5_2:14195659-14195678 | None:intergenic | 45.0% | |
| GCACGATGTGTGGTTTAAAG+GGG | + | chr5_2:14195773-14195792 | Msa0758820:intron | 45.0% | |
| GGGAATTTGGAGGAAGTTGT+TGG | + | chr5_2:14195793-14195812 | Msa0758820:CDS | 45.0% | |
| TGGACTGAAAGTCGAGTACA+CGG | + | chr5_2:14195999-14196018 | Msa0758820:intron | 45.0% | |
| ATTGTGATTGTGCAGGACCA+CGG | + | chr5_2:14196159-14196178 | Msa0758820:intron | 45.0% | |
| ACCACGGAAGAGTTTAATGC+TGG | + | chr5_2:14196175-14196194 | Msa0758820:CDS | 45.0% | |
| TCCAGCATTAAACTCTTCCG+TGG | - | chr5_2:14196179-14196198 | None:intergenic | 45.0% | |
| ! | TTTTGCAGGGTTGTCAGCTT+GGG | + | chr5_2:14196655-14196674 | Msa0758820:intron | 45.0% |
| GCTTGGGAAAAGGTCTATGA+TGG | + | chr5_2:14196671-14196690 | Msa0758820:CDS | 45.0% | |
| TGCATTGTATAGGGGTTCAC+CGG | + | chr5_2:14197066-14197085 | Msa0758820:intron | 45.0% | |
| TCTGAAGAAACCCTCTCTCT+TGG | + | chr5_2:14197291-14197310 | Msa0758820:three_prime_UTR | 45.0% | |
| ACACATCAAGCCAAGAGAGA+GGG | - | chr5_2:14197304-14197323 | None:intergenic | 45.0% | |
| AACACATCAAGCCAAGAGAG+AGG | - | chr5_2:14197305-14197324 | None:intergenic | 45.0% | |
| !!! | TATGTAATTTTTAAATTTAT+TGG | + | chr5_2:14195979-14195998 | Msa0758820:intron | 5.0% |
| ACGTGAATGAAAGCAAGCGG+CGG | - | chr5_2:14195403-14195422 | None:intergenic | 50.0% | |
| GGCAGAACACGACCATAACA+AGG | + | chr5_2:14195436-14195455 | Msa0758820:CDS | 50.0% | |
| TTTAATGCTGGACACGCTCC+TGG | + | chr5_2:14196187-14196206 | Msa0758820:CDS | 50.0% | |
| AAAATCTCATGGTGCGGCGA+AGG | - | chr5_2:14196585-14196604 | None:intergenic | 50.0% | |
| ! | CTTTTGCAGGGTTGTCAGCT+TGG | + | chr5_2:14196654-14196673 | Msa0758820:intron | 50.0% |
| AGGGTTGTCAGCTTGGGAAA+AGG | + | chr5_2:14196661-14196680 | Msa0758820:intron | 50.0% | |
| ACCAGCTATGTCTGTGAGTC+CGG | - | chr5_2:14197088-14197107 | None:intergenic | 50.0% | |
| TAGCTGGTGGTTATGCTGCT+TGG | + | chr5_2:14197100-14197119 | Msa0758820:CDS | 50.0% | |
| ! | ACACGCACTGGCACTGAAGT+TGG | - | chr5_2:14195823-14195842 | None:intergenic | 55.0% |
| GCTCACGAGCTTCTTCTAGC+TGG | + | chr5_2:14195844-14195863 | Msa0758820:CDS | 55.0% | |
| ACCGGACTCACAGACATAGC+TGG | + | chr5_2:14197084-14197103 | Msa0758820:CDS | 55.0% | |
| GGACTCACAGACATAGCTGG+TGG | + | chr5_2:14197087-14197106 | Msa0758820:CDS | 55.0% | |
| GCAAGCGGCGGAGACATCAA+AGG | - | chr5_2:14195391-14195410 | None:intergenic | 60.0% | |
| AGCTCGTGAGCTACACGCAC+TGG | - | chr5_2:14195835-14195854 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 14195168 | 14197644 | 14195168 | ID=Msa0758820;Name=Msa0758820 |
| chr5_2 | mRNA | 14195168 | 14197644 | 14195168 | ID=Msa0758820-mRNA-1;Parent=Msa0758820;Name=Msa0758820-mRNA-1;_AED=0.06;_eAED=0.06;_QI=198|1|1|1|1|1|7|492|185 |
| chr5_2 | exon | 14195168 | 14195457 | 14195168 | ID=Msa0758820-mRNA-1:exon:6406;Parent=Msa0758820-mRNA-1 |
| chr5_2 | exon | 14195598 | 14195675 | 14195598 | ID=Msa0758820-mRNA-1:exon:6407;Parent=Msa0758820-mRNA-1 |
| chr5_2 | exon | 14195774 | 14195887 | 14195774 | ID=Msa0758820-mRNA-1:exon:6408;Parent=Msa0758820-mRNA-1 |
| chr5_2 | exon | 14196174 | 14196247 | 14196174 | ID=Msa0758820-mRNA-1:exon:6409;Parent=Msa0758820-mRNA-1 |
| chr5_2 | exon | 14196499 | 14196569 | 14196499 | ID=Msa0758820-mRNA-1:exon:6410;Parent=Msa0758820-mRNA-1 |
| chr5_2 | exon | 14196663 | 14196716 | 14196663 | ID=Msa0758820-mRNA-1:exon:6411;Parent=Msa0758820-mRNA-1 |
| chr5_2 | exon | 14197078 | 14197644 | 14197078 | ID=Msa0758820-mRNA-1:exon:6412;Parent=Msa0758820-mRNA-1 |
| chr5_2 | five_prime_UTR | 14195168 | 14195365 | 14195168 | ID=Msa0758820-mRNA-1:five_prime_utr;Parent=Msa0758820-mRNA-1 |
| chr5_2 | CDS | 14195366 | 14195457 | 14195366 | ID=Msa0758820-mRNA-1:cds;Parent=Msa0758820-mRNA-1 |
| chr5_2 | CDS | 14195598 | 14195675 | 14195598 | ID=Msa0758820-mRNA-1:cds;Parent=Msa0758820-mRNA-1 |
| chr5_2 | CDS | 14195774 | 14195887 | 14195774 | ID=Msa0758820-mRNA-1:cds;Parent=Msa0758820-mRNA-1 |
| chr5_2 | CDS | 14196174 | 14196247 | 14196174 | ID=Msa0758820-mRNA-1:cds;Parent=Msa0758820-mRNA-1 |
| chr5_2 | CDS | 14196499 | 14196569 | 14196499 | ID=Msa0758820-mRNA-1:cds;Parent=Msa0758820-mRNA-1 |
| chr5_2 | CDS | 14196663 | 14196716 | 14196663 | ID=Msa0758820-mRNA-1:cds;Parent=Msa0758820-mRNA-1 |
| chr5_2 | CDS | 14197078 | 14197152 | 14197078 | ID=Msa0758820-mRNA-1:cds;Parent=Msa0758820-mRNA-1 |
| chr5_2 | three_prime_UTR | 14197153 | 14197644 | 14197153 | ID=Msa0758820-mRNA-1:three_prime_utr;Parent=Msa0758820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
