Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0638130 | XP_013443592.1 | 97.191 | 534 | 8 | 2 | 1 | 534 | 1 | 527 | 0.0 | 1033 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030380 | Msa0638130 | -0.818873 | 1.561583e-52 | -8.615850e-47 |
| Msa0107250 | Msa0638130 | -0.804979 | 1.665382e-49 | -8.615850e-47 |
| Msa0172350 | Msa0638130 | -0.814056 | 1.870715e-51 | -8.615850e-47 |
| Msa0173740 | Msa0638130 | -0.819226 | 1.297928e-52 | -8.615850e-47 |
| Msa0173820 | Msa0638130 | -0.824245 | 8.959390e-54 | -8.615850e-47 |
| Msa0173830 | Msa0638130 | -0.809097 | 2.238845e-50 | -8.615850e-47 |
| Msa0242680 | Msa0638130 | -0.815176 | 1.057159e-51 | -8.615850e-47 |
| Msa0267760 | Msa0638130 | -0.811479 | 6.857284e-51 | -8.615850e-47 |
| Msa0372660 | Msa0638130 | -0.804448 | 2.149750e-49 | -8.615850e-47 |
| Msa0372670 | Msa0638130 | -0.823009 | 1.744006e-53 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0638130 | MtrunA17_Chr4g0021121 | 97.191 | 534 | 8 | 2 | 1 | 534 | 1 | 527 | 0.0 | 1033 |
| Msa0638130 | MtrunA17_Chr2g0324351 | 54.662 | 547 | 188 | 12 | 1 | 534 | 1 | 500 | 7.10e-167 | 482 |
| Msa0638130 | MtrunA17_Chr6g0449531 | 31.546 | 317 | 166 | 14 | 12 | 307 | 59 | 345 | 2.47e-21 | 97.4 |
| Msa0638130 | MtrunA17_Chr2g0324371 | 42.553 | 141 | 28 | 5 | 21 | 159 | 13 | 102 | 1.89e-18 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0638130 | AT4G28300.1 | 50.628 | 557 | 189 | 15 | 1 | 532 | 1 | 496 | 5.93e-137 | 406 |
| Msa0638130 | AT4G28300.2 | 50.101 | 493 | 173 | 12 | 58 | 532 | 1 | 438 | 8.38e-113 | 342 |
| Msa0638130 | AT5G14540.1 | 33.813 | 139 | 88 | 1 | 61 | 199 | 111 | 245 | 2.68e-21 | 97.8 |
| Msa0638130 | AT3G01560.2 | 36.364 | 99 | 61 | 1 | 84 | 182 | 5 | 101 | 4.35e-17 | 83.6 |
| Msa0638130 | AT3G01560.1 | 36.364 | 99 | 61 | 1 | 84 | 182 | 134 | 230 | 1.37e-16 | 83.2 |
Find 201 sgRNAs with CRISPR-Local
Find 347 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGTCGCCTGGTTGGGAA+GGG | 0.585185 | 4_3:+32091291 | None:intergenic |
| AAATGAGAGGGTTGGGGTGG+TGG | 0.351350 | 4_3:+32091176 | None:intergenic |
| AACAGTAAATGACAATTCCA+CGG | 0.550242 | 4_3:+32090870 | None:intergenic |
| AAGAACAGAGTTGAAATCTA+TGG | 0.362354 | 4_3:+32090965 | None:intergenic |
| AAGCCATTCACAGTCCAATG+AGG | 0.674286 | 4_3:-32091951 | Msa0638130:CDS |
| AAGGCATATACTGATTCTGG+GGG | 0.685244 | 4_3:+32091717 | None:intergenic |
| AATACCTTGGTTGAAATGGA+AGG | 0.582478 | 4_3:+32093944 | None:intergenic |
| AATCTTATGCGATTTCTCGA+GGG | 0.629595 | 4_3:-32093390 | Msa0638130:CDS |
| AATGAGCCCTTGATTTGCTG+GGG | 0.569260 | 4_3:+32091314 | None:intergenic |
| AATGCATATATGATGTATGA+TGG | 0.595623 | 4_3:-32091224 | Msa0638130:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTTGTGTAGAGTGTGTAAA+GGG | + | chr4_3:32090818-32090837 | None:intergenic | 30.0% | |
| AATTTGTGTAGAGTGTGTAA+AGG | + | chr4_3:32090819-32090838 | None:intergenic | 30.0% | |
| GTGATTATTGGAATTGGAAT+TGG | + | chr4_3:32090983-32091002 | None:intergenic | 30.0% | |
| ATCGTTGTGATTATTGGAAT+TGG | + | chr4_3:32090989-32091008 | None:intergenic | 30.0% | |
| AAAAAAAGTTATCGTTGACG+CGG | + | chr4_3:32091036-32091055 | None:intergenic | 30.0% | |
| TGGTAGAACAATTATTAGTC+AGG | - | chr4_3:32091241-32091260 | Msa0638130:CDS | 30.0% | |
| AAATGTAGGCAACACAAAAA+TGG | + | chr4_3:32091864-32091883 | None:intergenic | 30.0% | |
| AACACAATAAAATCAAGGAC+AGG | - | chr4_3:32092070-32092089 | Msa0638130:intron | 30.0% | |
| ACACAATAAAATCAAGGACA+GGG | - | chr4_3:32092071-32092090 | Msa0638130:intron | 30.0% | |
| GCTATCCTTCATTTAATATG+GGG | - | chr4_3:32092609-32092628 | Msa0638130:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 32090733 | 32094242 | 32090733 | ID=Msa0638130;Name=Msa0638130 |
| chr4_3 | mRNA | 32090733 | 32094242 | 32090733 | ID=Msa0638130-mRNA-1;Parent=Msa0638130;Name=Msa0638130-mRNA-1;_AED=0.01;_eAED=0.01;_QI=141|1|1|1|1|1|3|178|534 |
| chr4_3 | exon | 32090733 | 32092074 | 32090733 | ID=Msa0638130-mRNA-1:exon:7497;Parent=Msa0638130-mRNA-1 |
| chr4_3 | exon | 32093251 | 32093538 | 32093251 | ID=Msa0638130-mRNA-1:exon:7496;Parent=Msa0638130-mRNA-1 |
| chr4_3 | exon | 32093949 | 32094242 | 32093949 | ID=Msa0638130-mRNA-1:exon:7495;Parent=Msa0638130-mRNA-1 |
| chr4_3 | five_prime_UTR | 32094102 | 32094242 | 32094102 | ID=Msa0638130-mRNA-1:five_prime_utr;Parent=Msa0638130-mRNA-1 |
| chr4_3 | CDS | 32093949 | 32094101 | 32093949 | ID=Msa0638130-mRNA-1:cds;Parent=Msa0638130-mRNA-1 |
| chr4_3 | CDS | 32093251 | 32093538 | 32093251 | ID=Msa0638130-mRNA-1:cds;Parent=Msa0638130-mRNA-1 |
| chr4_3 | CDS | 32090911 | 32092074 | 32090911 | ID=Msa0638130-mRNA-1:cds;Parent=Msa0638130-mRNA-1 |
| chr4_3 | three_prime_UTR | 32090733 | 32090910 | 32090733 | ID=Msa0638130-mRNA-1:three_prime_utr;Parent=Msa0638130-mRNA-1 |
| Gene Sequence |
>Msa0638130 ATGGCTTCTGGATCATCTGGTCGCGGTAACCCCGGCTTCGATTTTGCATCCGATGACATCTTGTGCTCTTACGAAGACTTTTCCAATAGGGATTCCAATAGGGATTCCAATTCCAATTCCAATAATCACAACGATCCTTCCATTTCAACCAAGGATTTTCACAAATCAAGGGTAGCAAGGACATCTGTTTTTCCTGCTGCTGCCTATAATCCACCTGAAGATTCTTTAAGCCAAGATGTGATTGCAACTGTTGAAAAGAGTATGAAGAAATATGCTGACAATCTTATGCGATTTCTCGAGGGAATTAGTTCAAGGCTATCACAGTTGGAATTATATTGCTACAATCTTGACAAATCTATTGGAGAAATCAGATCTGATTTAAATCGTGACCATGGTGACCAAGACTCAAAGCTCAAATCTCTTGAAAAGCACGTTCAGGAAGTTCACAGGTCAGTACAAATTTTAAGAGACAAACAAGAGTTAGCCGAGACTCAGAAAGAGTTAGCCAAGCTTCAACTGGCTCAGAAAGAATCATCTTCCTCAAGCCATTCACAGTCCAATGAGGACAAATCTTCACCTTCTACAACAGATCCTAAAAAAGCTGATAATGCATCTGATACAAACAACCAGCAGTTAGCTCTTGCTCTGCCTCATCAAATTGCTCCTCAGCCGCAGCCTGCAGGACCCCCGCCGGCCCAAGCTCCACCAACAAATGTGAATCAACCCACTCAACAACCCTCTTATTACATGCCGCCTGCTCCTGCAGTGTCTCAGCTCCCCCAGAATCAGTATATGCCTTCTGATCAACAATATCGAACCCCCCAACTACAAGACATGTCCCGGGTGGCTCAACAACCTACACCATCTCAAATAAATCCCCCCACACCTGTGCAACAATTCTCTCAGTATCAGCAGCAGCCCCCGCCGCAGCAGCAGCAGCAATGGTCTCAGCAGGTCCAACAATCCTCAATGCAACCTCAAATGAGACCCCCCTCGTCTACTGTGTATCCTCCATACCAGCCCAGCCAAGCATCAAATCCATCACCCACAGAAACACTGCCAAACAGCATGCCCATGCAAATGCCATATTCAGGGGTTCCGCAACCAGGATCCAGCCGTGCTGATTCCATGCAATATGGATATGGGGGAACTGGTAGAACAGTTCCCCAACAGCCTCCACCCCAGCAAATCAAGGGCTCATTCCCTTCCCAACCAGGCGACGTTTATGGACCTAGCGGGACCCACCCGGCACTCCCTCCTCCTCCTGGTAATGCATATATGATGTATGATGGTGGTGAGGGAGGAAGGGCACATCATCCACCACCCCAACCCTCTCATTTTGCTCAATCTGGATATCCTCCAACAAGTGCTTCCCTTCAGAACCCCAATCTCCTGGTTCGAAATCCTAGCCAGTCACAGTTTGTACGCAACCATCCTTACAATGAGTTGATTGAAAAATTGGTGAACATGGGATTCAGGGGTGACCATGTGGCGAGCGTGATCCAAAGGATGGAGGAGAGTGGACAGACCATAGATTTCAACTCTGTTCTTGACAGGTTGAATGTGCATGGCTCTGTTGGCCCCCAGAGAGGGTGGTCAGGGTGA |
| Protein sequence |
>Msa0638130 MASGSSGRGNPGFDFASDDILCSYEDFSNRDSNRDSNSNSNNHNDPSISTKDFHKSRVARTSVFPAAAYNPPEDSLSQDVIATVEKSMKKYADNLMRFLEGISSRLSQLELYCYNLDKSIGEIRSDLNRDHGDQDSKLKSLEKHVQEVHRSVQILRDKQELAETQKELAKLQLAQKESSSSSHSQSNEDKSSPSTTDPKKADNASDTNNQQLALALPHQIAPQPQPAGPPPAQAPPTNVNQPTQQPSYYMPPAPAVSQLPQNQYMPSDQQYRTPQLQDMSRVAQQPTPSQINPPTPVQQFSQYQQQPPPQQQQQWSQQVQQSSMQPQMRPPSSTVYPPYQPSQASNPSPTETLPNSMPMQMPYSGVPQPGSSRADSMQYGYGGTGRTVPQQPPPQQIKGSFPSQPGDVYGPSGTHPALPPPPGNAYMMYDGGEGGRAHHPPPQPSHFAQSGYPPTSASLQNPNLLVRNPSQSQFVRNHPYNELIEKLVNMGFRGDHVASVIQRMEESGQTIDFNSVLDRLNVHGSVGPQRGWSG* |
