AlfalfaGEDB | Msa0874500

M. sativa cultivar zhongmu-4 / Msa0874500

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0874500	KEH25123.1	97.170	106	3	0	1	106	1	106	1.21e-69	213

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0874500	A0A072U7M0	97.170	106	3	0	1	106	1	106	5.78e-70	213

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0874500	-0.833057	6.634461e-56	-8.615850e-47
Msa0002540	Msa0874500	0.801440	8.995950e-49	-8.615850e-47
Msa0003470	Msa0874500	0.801243	9.869167e-49	-8.615850e-47
Msa0004980	Msa0874500	0.806885	6.620420e-50	-8.615850e-47
Msa0011720	Msa0874500	0.821280	4.391645e-53	-8.615850e-47
Msa0013050	Msa0874500	-0.818428	1.970193e-52	-8.615850e-47
Msa0015300	Msa0874500	0.831216	1.892734e-55	-8.615850e-47
Msa0023830	Msa0874500	-0.824131	9.531107e-54	-8.615850e-47
Msa0024680	Msa0874500	0.815527	8.833131e-52	-8.615850e-47
Msa0027540	Msa0874500	0.811877	5.618307e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0874500	MtrunA17_Chr6g0455241	95.402	87	4	0	20	106	56	142	7.72e-59	177
Msa0874500	MtrunA17_Chr6g0455271	95.876	97	4	0	1	97	1	97	1.14e-53	164

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 18 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACATCATTGAAGATAA+AGG	0.308741	6_1:-10312123	Msa0874500:CDS
ACAACATCATTGAAGATAAA+GGG	0.430981	6_1:-10312122	Msa0874500:CDS
ACATGCAGCAAGAATGCTTT+TGG	0.267933	6_1:-10312036	Msa0874500:CDS
AGAAGGCAAGCAACTACGAT+AGG	0.559482	6_1:+10311941	None:intergenic
AGTTGCTTGCCTTCTCAAAA+TGG	0.301353	6_1:-10311933	Msa0874500:CDS
ATGGAGGTGGACCTAACAAT+AGG	0.486497	6_1:-10311914	Msa0874500:CDS
CAAACACAAACTGGTAGAAC+TGG	0.449515	6_1:-10312005	Msa0874500:CDS
CTACGATAGGCTTGATCTAT+TGG	0.282742	6_1:+10311954	None:intergenic
GATAAAGGGTTGTACCTCAG+AGG	0.671194	6_1:-10312108	Msa0874500:CDS
GATTTGAGTCAAACACAAAC+TGG	0.464256	6_1:-10312014	Msa0874500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTCTCATCAACAATGATT+TGG	-	chr6_1:10311988-10312007	Msa0874500:CDS	30.0%
GATTTGAGTCAAACACAAAC+TGG	-	chr6_1:10312058-10312077	Msa0874500:CDS	35.0%
TAAGTACCACACCTATTGTT+AGG	+	chr6_1:10312172-10312191	None:intergenic	35.0%
CAAACACAAACTGGTAGAAC+TGG	-	chr6_1:10312067-10312086	Msa0874500:CDS	40.0%
GGTAGAACTGGTAACAACAA+TGG	-	chr6_1:10312079-10312098	Msa0874500:CDS	40.0%
CTACGATAGGCTTGATCTAT+TGG	+	chr6_1:10312121-10312140	None:intergenic	40.0%
AGTTGCTTGCCTTCTCAAAA+TGG	-	chr6_1:10312139-10312158	Msa0874500:CDS	40.0%
GATAAAGGGTTGTACCTCAG+AGG	-	chr6_1:10311964-10311983	Msa0874500:CDS	45.0%
AGAAGGCAAGCAACTACGAT+AGG	+	chr6_1:10312134-10312153	None:intergenic	45.0%
TGCTTGCCTTCTCAAAATGG+AGG	-	chr6_1:10312142-10312161	Msa0874500:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	10311887	10312207	10311887	ID=Msa0874500;Name=Msa0874500
chr6_1	mRNA	10311887	10312207	10311887	ID=Msa0874500-mRNA-1;Parent=Msa0874500;Name=Msa0874500-mRNA-1;_AED=0.27;_eAED=0.28;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|106
chr6_1	exon	10311887	10312207	10311887	ID=Msa0874500-mRNA-1:exon:3205;Parent=Msa0874500-mRNA-1
chr6_1	CDS	10311887	10312207	10311887	ID=Msa0874500-mRNA-1:cds;Parent=Msa0874500-mRNA-1

Gene Sequence	>Msa0874500 ATGATGAACAAGGGTGTATCACTATTGTTCCTTATCTTATTGATCCAATTACATCTAAGCAAAACAACATCATTGAAGATAAAGGGTTGTACCTCAGAGGATTGTCTCATCAACAATGATTTGGAATCAGAAGAATTTTCTATGAGTTCACATGCAGCAAGAATGCTTTTGGATTTGAGTCAAACACAAACTGGTAGAACTGGTAACAACAATGGTGCTGCTGTTAAGTGTCCAATAGATCAAGCCTATCGTAGTTGCTTGCCTTCTCAAAATGGAGGTGGACCTAACAATAGGTGTGGTACTTACAATAGAGTTTGCTAA
Protein sequence	>Msa0874500 MMNKGVSLLFLILLIQLHLSKTTSLKIKGCTSEDCLINNDLESEEFSMSSHAARMLLDLSQTQTGRTGNNNGAAVKCPIDQAYRSCLPSQNGGGPNNRCGTYNRVC*