AlfalfaGEDB | Msa0659540

M. sativa cultivar zhongmu-4 / Msa0659540

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659540	AET04830.2	49.239	197	59	8	3	178	98	274	9.26e-40	147

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0319170	Msa0659540	0.805381	1.372079e-49	-8.615850e-47
Msa0363790	Msa0659540	0.807877	4.077144e-50	-8.615850e-47
Msa0379640	Msa0659540	0.816728	4.762010e-52	-8.615850e-47
Msa0467060	Msa0659540	0.801581	8.416395e-49	-8.615850e-47
Msa0481530	Msa0659540	0.802267	6.084057e-49	-8.615850e-47
Msa0484780	Msa0659540	0.820097	8.213425e-53	-8.615850e-47
Msa0495320	Msa0659540	0.801897	7.248005e-49	-8.615850e-47
Msa0512750	Msa0659540	0.819802	9.591413e-53	-8.615850e-47
Msa0512900	Msa0659540	0.805377	1.374949e-49	-8.615850e-47
Msa0558690	Msa0659540	0.800903	1.158529e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659540	MtrunA17_Chr8g0384811	78.378	37	8	0	93	129	1	37	5.84e-14	63.9
Msa0659540	MtrunA17_Chr4g0003561	71.429	49	11	1	84	129	197	245	8.83e-14	68.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 48 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTACTGAACATCGTCTA+AGG	0.315346	4_3:+69934646	None:intergenic
AAGTACTGAACATCGTCTAA+GGG	0.580680	4_3:+69934647	None:intergenic
ACGAAGGTAGAGAGACAAAA+AGG	0.292768	4_3:+69934191	None:intergenic
ACTCTACGATTGGAAACCAA+TGG	0.567217	4_3:-69934759	Msa0659540:CDS
AGAAATCTTCTTCGTTGAGA+AGG	0.512326	4_3:+69933728	None:intergenic
AGAGCAGCTCTTGCATATAA+AGG	0.385108	4_3:+69934288	None:intergenic
ATATACTCTGATATATCCAT+TGG	0.503147	4_3:+69934743	None:intergenic
ATATCAGAGTATATTGCTAC+TGG	0.451236	4_3:-69934734	Msa0659540:CDS
CAAAGGGTCTCCATGGCAAG+GGG	0.637668	4_3:+69935345	None:intergenic
CATTGCATCTAGAAGCTTAG+AGG	0.363803	4_3:+69934221	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCAATCATTCAAACACAAAT+AGG	+	chr4_3:69934033-69934052	None:intergenic	30.0%
CCTATTTGTGTTTGAATGAT+TGG	-	chr4_3:69934030-69934049	Msa0659540:intron	30.0%
CAACTCAAGAAATCTTACTT+TGG	-	chr4_3:69934102-69934121	Msa0659540:intron	30.0%
TTGGCTAATGAAATTTGCAA+TGG	-	chr4_3:69934121-69934140	Msa0659540:intron	30.0%
ATATCAGAGTATATTGCTAC+TGG	-	chr4_3:69934274-69934293	Msa0659540:CDS	30.0%
TATCAGAGTATATTGCTACT+GGG	-	chr4_3:69934275-69934294	Msa0659540:CDS	30.0%
CCAATCATTCAAACACAAAT+AGG	+	chr4_3:69934033-69934052	None:intergenic	30.0%
CCTATTTGTGTTTGAATGAT+TGG	-	chr4_3:69934030-69934049	Msa0659540:intron	30.0%
CAACTCAAGAAATCTTACTT+TGG	-	chr4_3:69934102-69934121	Msa0659540:intron	30.0%
TTGGCTAATGAAATTTGCAA+TGG	-	chr4_3:69934121-69934140	Msa0659540:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	69933642	69935388	69933642	ID=Msa0659540;Name=Msa0659540
chr4_3	mRNA	69933642	69935388	69933642	ID=Msa0659540-mRNA-1;Parent=Msa0659540;Name=Msa0659540-mRNA-1;_AED=0.47;_eAED=0.61;_QI=0\|0\|0\|1\|0.33\|0.25\|4\|0\|189
chr4_3	exon	69935271	69935388	69935271	ID=Msa0659540-mRNA-1:exon:20668;Parent=Msa0659540-mRNA-1
chr4_3	exon	69934653	69934785	69934653	ID=Msa0659540-mRNA-1:exon:20667;Parent=Msa0659540-mRNA-1
chr4_3	exon	69934176	69934314	69934176	ID=Msa0659540-mRNA-1:exon:20666;Parent=Msa0659540-mRNA-1
chr4_3	exon	69933642	69933821	69933642	ID=Msa0659540-mRNA-1:exon:20665;Parent=Msa0659540-mRNA-1
chr4_3	CDS	69935271	69935388	69935271	ID=Msa0659540-mRNA-1:cds;Parent=Msa0659540-mRNA-1
chr4_3	CDS	69934653	69934785	69934653	ID=Msa0659540-mRNA-1:cds;Parent=Msa0659540-mRNA-1
chr4_3	CDS	69934176	69934314	69934176	ID=Msa0659540-mRNA-1:cds;Parent=Msa0659540-mRNA-1
chr4_3	CDS	69933642	69933821	69933642	ID=Msa0659540-mRNA-1:cds;Parent=Msa0659540-mRNA-1

Gene Sequence

>Msa0659540

ATGAATTGGTGGGGTTGCAGACCCCTTGCCATGGAGACCCTTTGCAATTGGTGGCTACAAGGGCATCAACCAGGTGCTTTTTGCATAGTCAAATCATATTTGGTTCTACTGACTTCAACTCTACTCTACGATTGGAAACCAATGGATATATCAGAGTATATTGCTACTGGGTCTCAATCAGATGCAAAGAGATACGAGTGCTTGAAGAGAACTCTCTTGAATTTTAATTTCAGGCCCTTAGACGATGTTCAATATCCTTTATATGCAAGAGCTGCTCTGTGTCAAGCTTGTTGCAAGGTGGCCAAACCCTTGGAACCAGGAGCCTCTAAGCTTCTAGATGCAATGTGTAAACCCTTTTTGTCTCTCTACCTTCGTTTGGAGCCCGATAAGATGGTCTTATTGGAACTGATGGAAATTGAAGGGTTTAACAGATATTTACACATCATATTCACCAATTCTAGCCTTCTCAACGAAGAAGATTTCTCAAGTATACATGCAAAAGCTGTTTTGGATTATTTTATATCTATTGAGAGCAGAATTCAAAATGTTATAATCAAGAAAATAAAATAG

Protein sequence

>Msa0659540

MNWWGCRPLAMETLCNWWLQGHQPGAFCIVKSYLVLLTSTLLYDWKPMDISEYIATGSQSDAKRYECLKRTLLNFNFRPLDDVQYPLYARAALCQACCKVAKPLEPGASKLLDAMCKPFLSLYLRLEPDKMVLLELMEIEGFNRYLHIIFTNSSLLNEEDFSSIHAKAVLDYFISIESRIQNVIIKKIK*