Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665150 | XP_003604378.1 | 99.000 | 100 | 1 | 0 | 1 | 100 | 112 | 211 | 3.84e-66 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665150 | sp|Q9LVZ8|LOR12_ARATH | 54.545 | 99 | 43 | 1 | 2 | 100 | 124 | 220 | 4.49e-29 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665150 | B7FGI9 | 99.000 | 100 | 1 | 0 | 1 | 100 | 112 | 211 | 1.84e-66 | 208 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0096020 | Msa0665150 | 0.831155 | 1.959906e-55 | -8.615850e-47 |
| Msa0614980 | Msa0665150 | 0.804368 | 2.234183e-49 | -8.615850e-47 |
| Msa0621780 | Msa0665150 | 0.822846 | 1.903776e-53 | -8.615850e-47 |
| Msa0665150 | Msa0715510 | -0.809327 | 1.998795e-50 | -8.615850e-47 |
| Msa0665150 | Msa0748980 | 0.810589 | 1.069238e-50 | -8.615850e-47 |
| Msa0665150 | Msa0754350 | 0.842676 | 2.235790e-58 | -8.615850e-47 |
| Msa0665150 | Msa0756440 | 0.808911 | 2.453608e-50 | -8.615850e-47 |
| Msa0665150 | Msa0795710 | 0.824649 | 7.197964e-54 | -8.615850e-47 |
| Msa0665150 | Msa0833450 | 0.810780 | 9.722729e-51 | -8.615850e-47 |
| Msa0665150 | Msa0835550 | 0.812696 | 3.722761e-51 | -8.615850e-47 |
| Msa0665150 | Msa1206760 | 0.803860 | 2.848831e-49 | -8.615850e-47 |
| Msa0665150 | Msa1206830 | 0.815550 | 8.730507e-52 | -8.615850e-47 |
| Msa0665150 | Msa1242990 | 0.844817 | 5.977655e-59 | -8.615850e-47 |
| Msa0665150 | Msa1257100 | 0.805413 | 1.351225e-49 | -8.615850e-47 |
| Msa0665150 | Msa1278420 | 0.812085 | 5.062441e-51 | -8.615850e-47 |
| Msa0665150 | Msa1284390 | 0.819748 | 9.867178e-53 | -8.615850e-47 |
| Msa0665150 | Msa1330910 | 0.802657 | 5.056660e-49 | -8.615850e-47 |
| Msa0665150 | Msa1403950 | 0.827510 | 1.503761e-54 | -8.615850e-47 |
| Msa0665150 | Msa1406990 | 0.805618 | 1.223601e-49 | -8.615850e-47 |
| Msa0665150 | Msa1452200 | 0.811010 | 8.670746e-51 | -8.615850e-47 |
| Msa0230980 | Msa0665150 | 0.807874 | 4.084863e-50 | -8.615850e-47 |
| Msa0412760 | Msa0665150 | 0.811100 | 8.290016e-51 | -8.615850e-47 |
| Msa0481050 | Msa0665150 | 0.821088 | 4.863424e-53 | -8.615850e-47 |
| Msa0270460 | Msa0665150 | 0.806365 | 8.521168e-50 | -8.615850e-47 |
| Msa0500490 | Msa0665150 | 0.805305 | 1.423465e-49 | -8.615850e-47 |
| Msa0530380 | Msa0665150 | 0.887247 | 1.687435e-72 | -8.615850e-47 |
| Msa0545230 | Msa0665150 | 0.800169 | 1.635139e-48 | -8.615850e-47 |
| Msa0580540 | Msa0665150 | 0.824519 | 7.724803e-54 | -8.615850e-47 |
| Msa0587290 | Msa0665150 | 0.963572 | 3.000856e-122 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665150 | MtrunA17_Chr4g0003271 | 99.000 | 100 | 1 | 0 | 1 | 100 | 112 | 211 | 3.53e-70 | 208 |
| Msa0665150 | MtrunA17_Chr2g0333101 | 68.687 | 99 | 30 | 1 | 3 | 100 | 125 | 223 | 6.05e-44 | 142 |
| Msa0665150 | MtrunA17_Chr2g0333111 | 67.677 | 99 | 31 | 1 | 3 | 100 | 126 | 224 | 9.50e-44 | 141 |
| Msa0665150 | MtrunA17_Chr8g0387561 | 46.479 | 71 | 38 | 0 | 9 | 79 | 161 | 231 | 2.60e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665150 | AT3G15810.1 | 54.545 | 99 | 43 | 1 | 2 | 100 | 124 | 220 | 4.56e-30 | 107 |
| Msa0665150 | AT1G80120.1 | 57.426 | 101 | 39 | 3 | 2 | 100 | 111 | 209 | 8.17e-30 | 105 |
| Msa0665150 | AT5G41590.1 | 44.776 | 67 | 36 | 1 | 14 | 79 | 153 | 219 | 8.92e-11 | 56.6 |
Find 35 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGAGAATTTCTTTGATAA+TGG | 0.240720 | 4_4:+4546628 | Msa0665150:CDS |
| CTTTGCCATGGGATTGGTTT+TGG | 0.269542 | 4_4:+4546588 | Msa0665150:CDS |
| GTGAGAATTTCTTTGATAAT+GGG | 0.336997 | 4_4:+4546629 | Msa0665150:CDS |
| ATTCATCAATCTTCTGTAGT+AGG | 0.340424 | 4_4:-4546673 | None:intergenic |
| CTCAGCCACTACTTTCTTTG+TGG | 0.350694 | 4_4:-4546479 | None:intergenic |
| AAAATCTTACAGCATCTTTG+TGG | 0.417016 | 4_4:-4546451 | None:intergenic |
| TGTGTCCTTTGCCATGGGAT+TGG | 0.448534 | 4_4:+4546582 | Msa0665150:CDS |
| AAATCTTACAGCATCTTTGT+GGG | 0.461184 | 4_4:-4546450 | None:intergenic |
| TTGGTACTTGATCAGATCAA+TGG | 0.468589 | 4_4:+4546607 | Msa0665150:CDS |
| GCAAAGGACACATCAAAATC+AGG | 0.471041 | 4_4:-4546571 | None:intergenic |
| ATCGTGTACCTCCACTGTTA+TGG | 0.509066 | 4_4:-4546392 | None:intergenic |
| TTTGATGTGTCCTTTGCCAT+GGG | 0.516078 | 4_4:+4546577 | Msa0665150:CDS |
| TTCATCAATCTTCTGTAGTA+GGG | 0.533840 | 4_4:-4546672 | None:intergenic |
| GGTGTGGAGTACCTCATTGA+AGG | 0.534346 | 4_4:+4546421 | Msa0665150:CDS |
| TCAATGAGGTACTCCACACC+TGG | 0.537802 | 4_4:-4546418 | None:intergenic |
| AGGGTGCACCGAGGTTTCAA+TGG | 0.544254 | 4_4:-4546653 | None:intergenic |
| TAATGCCACAAAGAAAGTAG+TGG | 0.567237 | 4_4:+4546474 | Msa0665150:CDS |
| AGTGGCTGAGATTCGTCGTA+AGG | 0.580670 | 4_4:+4546492 | Msa0665150:CDS |
| ACCGAGCATAACACTAGTGG+TGG | 0.583087 | 4_4:-4546521 | None:intergenic |
| GGCTGAGATTCGTCGTAAGG+TGG | 0.591532 | 4_4:+4546495 | Msa0665150:CDS |
| GTGTGGAGTACCTCATTGAA+GGG | 0.603117 | 4_4:+4546422 | Msa0665150:CDS |
| TAATGGGACCATTGAAACCT+CGG | 0.618557 | 4_4:+4546645 | Msa0665150:CDS |
| GTGGGAAACACCCTTCAATG+AGG | 0.622701 | 4_4:-4546432 | None:intergenic |
| CCCACCACTAGTGTTATGCT+CGG | 0.625140 | 4_4:+4546520 | Msa0665150:CDS |
| AAGTACCAAAACCAATCCCA+TGG | 0.637046 | 4_4:-4546593 | None:intergenic |
| CAAAACCAATCCCATGGCAA+AGG | 0.650353 | 4_4:-4546587 | None:intergenic |
| TTTACCGAGCATAACACTAG+TGG | 0.651825 | 4_4:-4546524 | None:intergenic |
| CGAGCATAACACTAGTGGTG+GGG | 0.652992 | 4_4:-4546519 | None:intergenic |
| CCGAGCATAACACTAGTGGT+GGG | 0.663464 | 4_4:-4546520 | None:intergenic |
| GGTACACGATAACCCAGGTG+TGG | 0.667191 | 4_4:+4546405 | Msa0665150:CDS |
| AAGAATGACCATAACAGTGG+AGG | 0.694861 | 4_4:+4546384 | None:intergenic |
| GTCAAGAATGACCATAACAG+TGG | 0.699668 | 4_4:+4546381 | None:intergenic |
| CAATGAGGTACTCCACACCT+GGG | 0.718093 | 4_4:-4546417 | None:intergenic |
| TTCTGTAGTAGGGTGCACCG+AGG | 0.725146 | 4_4:-4546662 | None:intergenic |
| GTGGAGGTACACGATAACCC+AGG | 0.734091 | 4_4:+4546400 | Msa0665150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTGAGAATTTCTTTGATAAT+GGG | + | chr4_4:4546629-4546648 | Msa0665150:CDS | 25.0% |
| AAATCTTACAGCATCTTTGT+GGG | - | chr4_4:4546453-4546472 | None:intergenic | 30.0% | |
| AAAATCTTACAGCATCTTTG+TGG | - | chr4_4:4546454-4546473 | None:intergenic | 30.0% | |
| GGTGAGAATTTCTTTGATAA+TGG | + | chr4_4:4546628-4546647 | Msa0665150:CDS | 30.0% | |
| TAATGCCACAAAGAAAGTAG+TGG | + | chr4_4:4546474-4546493 | Msa0665150:CDS | 35.0% | |
| TTGGTACTTGATCAGATCAA+TGG | + | chr4_4:4546607-4546626 | Msa0665150:CDS | 35.0% | |
| TTTACCGAGCATAACACTAG+TGG | - | chr4_4:4546527-4546546 | None:intergenic | 40.0% | |
| GCAAAGGACACATCAAAATC+AGG | - | chr4_4:4546574-4546593 | None:intergenic | 40.0% | |
| ! | TTTTGATGTGTCCTTTGCCA+TGG | + | chr4_4:4546576-4546595 | Msa0665150:CDS | 40.0% |
| TTTGATGTGTCCTTTGCCAT+GGG | + | chr4_4:4546577-4546596 | Msa0665150:CDS | 40.0% | |
| AAGTACCAAAACCAATCCCA+TGG | - | chr4_4:4546596-4546615 | None:intergenic | 40.0% | |
| TAATGGGACCATTGAAACCT+CGG | + | chr4_4:4546645-4546664 | Msa0665150:CDS | 40.0% | |
| ATCGTGTACCTCCACTGTTA+TGG | - | chr4_4:4546395-4546414 | None:intergenic | 45.0% | |
| ! | GTGTGGAGTACCTCATTGAA+GGG | + | chr4_4:4546422-4546441 | Msa0665150:CDS | 45.0% |
| CTCAGCCACTACTTTCTTTG+TGG | - | chr4_4:4546482-4546501 | None:intergenic | 45.0% | |
| CAAAACCAATCCCATGGCAA+AGG | - | chr4_4:4546590-4546609 | None:intergenic | 45.0% | |
| !!! | CTTTGCCATGGGATTGGTTT+TGG | + | chr4_4:4546588-4546607 | Msa0665150:CDS | 45.0% |
| ! | CAATGAGGTACTCCACACCT+GGG | - | chr4_4:4546420-4546439 | None:intergenic | 50.0% |
| ! | TCAATGAGGTACTCCACACC+TGG | - | chr4_4:4546421-4546440 | None:intergenic | 50.0% |
| ! | GGTGTGGAGTACCTCATTGA+AGG | + | chr4_4:4546421-4546440 | Msa0665150:CDS | 50.0% |
| GTGGGAAACACCCTTCAATG+AGG | - | chr4_4:4546435-4546454 | None:intergenic | 50.0% | |
| AGTGGCTGAGATTCGTCGTA+AGG | + | chr4_4:4546492-4546511 | Msa0665150:CDS | 50.0% | |
| CGAGCATAACACTAGTGGTG+GGG | - | chr4_4:4546522-4546541 | None:intergenic | 50.0% | |
| CCGAGCATAACACTAGTGGT+GGG | - | chr4_4:4546523-4546542 | None:intergenic | 50.0% | |
| CCCACCACTAGTGTTATGCT+CGG | + | chr4_4:4546520-4546539 | Msa0665150:CDS | 50.0% | |
| ACCGAGCATAACACTAGTGG+TGG | - | chr4_4:4546524-4546543 | None:intergenic | 50.0% | |
| TGTGTCCTTTGCCATGGGAT+TGG | + | chr4_4:4546582-4546601 | Msa0665150:CDS | 50.0% | |
| ! | GTGGAGGTACACGATAACCC+AGG | + | chr4_4:4546400-4546419 | Msa0665150:CDS | 55.0% |
| GGTACACGATAACCCAGGTG+TGG | + | chr4_4:4546405-4546424 | Msa0665150:CDS | 55.0% | |
| GGCTGAGATTCGTCGTAAGG+TGG | + | chr4_4:4546495-4546514 | Msa0665150:CDS | 55.0% | |
| AGGGTGCACCGAGGTTTCAA+TGG | - | chr4_4:4546656-4546675 | None:intergenic | 55.0% | |
| TTCTGTAGTAGGGTGCACCG+AGG | - | chr4_4:4546665-4546684 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 4546388 | 4546690 | 4546388 | ID=Msa0665150;Name=Msa0665150 |
| chr4_4 | mRNA | 4546388 | 4546690 | 4546388 | ID=Msa0665150-mRNA-1;Parent=Msa0665150;Name=Msa0665150-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|-1|0|1|-1|1|1|0|100 |
| chr4_4 | exon | 4546388 | 4546690 | 4546388 | ID=Msa0665150-mRNA-1:exon:1519;Parent=Msa0665150-mRNA-1 |
| chr4_4 | CDS | 4546388 | 4546690 | 4546388 | ID=Msa0665150-mRNA-1:cds;Parent=Msa0665150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
