Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0062860 | Msa0687070 | 0.800764 | 1.236821e-48 | -8.615850e-47 |
| Msa0657970 | Msa0687070 | 0.815131 | 1.081895e-51 | -8.615850e-47 |
| Msa0673460 | Msa0687070 | 0.804886 | 1.741691e-49 | -8.615850e-47 |
| Msa0687070 | Msa0766630 | 0.824535 | 7.659935e-54 | -8.615850e-47 |
| Msa0687070 | Msa0767760 | 0.800540 | 1.373832e-48 | -8.615850e-47 |
| Msa0687070 | Msa0769920 | 0.800052 | 1.726545e-48 | -8.615850e-47 |
| Msa0687070 | Msa0806090 | 0.806470 | 8.100513e-50 | -8.615850e-47 |
| Msa0687070 | Msa0820850 | 0.811394 | 7.157515e-51 | -8.615850e-47 |
| Msa0687070 | Msa0861130 | 0.812285 | 4.579009e-51 | -8.615850e-47 |
| Msa0687070 | Msa0927290 | 0.805654 | 1.202506e-49 | -8.615850e-47 |
| Msa0687070 | Msa0927380 | 0.801294 | 9.636052e-49 | -8.615850e-47 |
| Msa0687070 | Msa0954910 | 0.831516 | 1.597322e-55 | -8.615850e-47 |
| Msa0687070 | Msa0969520 | 0.819645 | 1.041833e-52 | -8.615850e-47 |
| Msa0687070 | Msa1014540 | 0.820841 | 5.541358e-53 | -8.615850e-47 |
| Msa0687070 | Msa1075220 | -0.801796 | 7.604077e-49 | -8.615850e-47 |
| Msa0687070 | Msa1082500 | 0.817245 | 3.645349e-52 | -8.615850e-47 |
| Msa0687070 | Msa1174040 | 0.814564 | 1.444616e-51 | -8.615850e-47 |
| Msa0687070 | Msa1277660 | 0.809678 | 1.680272e-50 | -8.615850e-47 |
| Msa0687070 | Msa1284680 | 0.809486 | 1.847947e-50 | -8.615850e-47 |
| Msa0687070 | Msa1342840 | 0.872565 | 2.884585e-67 | -8.615850e-47 |
| Msa0687070 | Msa1426470 | 0.801073 | 1.069575e-48 | -8.615850e-47 |
| Msa0131770 | Msa0687070 | 0.806676 | 7.326835e-50 | -8.615850e-47 |
| Msa0142220 | Msa0687070 | 0.806703 | 7.231875e-50 | -8.615850e-47 |
| Msa0158480 | Msa0687070 | 0.809294 | 2.031989e-50 | -8.615850e-47 |
| Msa0231000 | Msa0687070 | 0.809118 | 2.215814e-50 | -8.615850e-47 |
| Msa0344910 | Msa0687070 | 0.800272 | 1.558148e-48 | -8.615850e-47 |
| Msa0512750 | Msa0687070 | 0.800741 | 1.250248e-48 | -8.615850e-47 |
| Msa0532350 | Msa0687070 | 0.813722 | 2.216397e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTGCAGGGATGACGGTTT+GGG | 0.226189 | 4_4:-51754852 | Msa0687070:CDS |
| TCATTATACCTAACATAAAT+TGG | 0.230942 | 4_4:+51754760 | None:intergenic |
| TTTGTGCAGGGATGACGGTT+TGG | 0.330848 | 4_4:-51754853 | Msa0687070:CDS |
| TTGAGTTTCTAAGCAAATAA+TGG | 0.353733 | 4_4:-51754452 | Msa0687070:three_prime_UTR |
| GTGGATGTCCAATTTATGTT+AGG | 0.355114 | 4_4:-51754768 | Msa0687070:intron |
| AGAAACTATCTAATGTTTCC+AGG | 0.375467 | 4_4:-51754489 | Msa0687070:three_prime_UTR |
| TGTGGCGCAACTCATGAAAT+TGG | 0.429611 | 4_4:-51754790 | Msa0687070:CDS |
| TAGGTTTATGCATACAAATC+AGG | 0.431471 | 4_4:-51754552 | Msa0687070:three_prime_UTR |
| TCAAGGATCTTTAGATGCCC+TGG | 0.444067 | 4_4:+51754471 | None:intergenic |
| TGCTATTAGCTGGTGTTGGT+TGG | 0.471070 | 4_4:-51754529 | Msa0687070:three_prime_UTR |
| CTTTGATCTTCTTAAGAAGC+TGG | 0.474951 | 4_4:-51754622 | Msa0687070:CDS |
| TGACAGACTAACATAAAGAA+TGG | 0.487712 | 4_4:+51754408 | None:intergenic |
| GCATTGTCTCTGCCATCATA+TGG | 0.506035 | 4_4:-51754823 | Msa0687070:CDS |
| ACAAATCAGGTGCTATTAGC+TGG | 0.518107 | 4_4:-51754539 | Msa0687070:three_prime_UTR |
| TGTGCAGGGATGACGGTTTG+GGG | 0.521080 | 4_4:-51754851 | Msa0687070:CDS |
| ATTATTTGCTTAGAAACTCA+AGG | 0.529892 | 4_4:+51754454 | None:intergenic |
| GATCTTCTTAAGAAGCTGGC+AGG | 0.533432 | 4_4:-51754618 | Msa0687070:CDS |
| CATGAGTTGCGCCACATCAA+AGG | 0.541976 | 4_4:+51754797 | None:intergenic |
| AGATCAAAGTTGAGGTTGCG+TGG | 0.567107 | 4_4:+51754636 | None:intergenic |
| CATCAAAGGCTACCATATGA+TGG | 0.567903 | 4_4:+51754811 | None:intergenic |
| TCTTAAGAAGATCAAAGTTG+AGG | 0.571010 | 4_4:+51754628 | None:intergenic |
| GAAACTATCTAATGTTTCCA+GGG | 0.584202 | 4_4:-51754488 | Msa0687070:three_prime_UTR |
| AACCTAGTCGTATCTCTCGT+CGG | 0.594968 | 4_4:+51754569 | None:intergenic |
| CAGGTGCTATTAGCTGGTGT+TGG | 0.611664 | 4_4:-51754533 | Msa0687070:three_prime_UTR |
| GGCGCAACTCATGAAATTGG+TGG | 0.617859 | 4_4:-51754787 | Msa0687070:CDS |
| TCATATGGTAGCCTTTGATG+TGG | 0.643558 | 4_4:-51754808 | Msa0687070:CDS |
| ATCCGACGAGAGATACGACT+AGG | 0.699549 | 4_4:-51754571 | Msa0687070:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATTATACCTAACATAAAT+TGG | + | chr4_4:51754540-51754559 | None:intergenic | 20.0% |
| !! | TTTATACACATTCATGAAAT+GGG | - | chr4_4:51754581-51754600 | Msa0687070:three_prime_UTR | 20.0% |
| !!! | TTTTGTTTTTATCTCTATGA+TGG | - | chr4_4:51754623-51754642 | Msa0687070:CDS | 20.0% |
| !!! | TTTTATTGTTGTTTCTAGAC+AGG | - | chr4_4:51754420-51754439 | Msa0687070:three_prime_UTR | 25.0% |
| ! | CTTTATACACATTCATGAAA+TGG | - | chr4_4:51754580-51754599 | Msa0687070:three_prime_UTR | 25.0% |
| ! | ATTATTTGCTTAGAAACTCA+AGG | + | chr4_4:51754846-51754865 | None:intergenic | 25.0% |
| ! | TTGAGTTTCTAAGCAAATAA+TGG | - | chr4_4:51754845-51754864 | Msa0687070:CDS | 25.0% |
| !!! | TATGTTTTGATGACTCTTAC+TGG | - | chr4_4:51754391-51754410 | Msa0687070:three_prime_UTR | 30.0% |
| TGAAATGGGCATAGATTTAA+TGG | - | chr4_4:51754595-51754614 | Msa0687070:exon | 30.0% | |
| TCTTAAGAAGATCAAAGTTG+AGG | + | chr4_4:51754672-51754691 | None:intergenic | 30.0% | |
| TAGGTTTATGCATACAAATC+AGG | - | chr4_4:51754745-51754764 | Msa0687070:intron | 30.0% | |
| AGAAACTATCTAATGTTTCC+AGG | - | chr4_4:51754808-51754827 | Msa0687070:CDS | 30.0% | |
| GAAACTATCTAATGTTTCCA+GGG | - | chr4_4:51754809-51754828 | Msa0687070:CDS | 30.0% | |
| TGACAGACTAACATAAAGAA+TGG | + | chr4_4:51754892-51754911 | None:intergenic | 30.0% | |
| GTGGATGTCCAATTTATGTT+AGG | - | chr4_4:51754529-51754548 | Msa0687070:three_prime_UTR | 35.0% | |
| AATGGGCATAGATTTAATGG+AGG | - | chr4_4:51754598-51754617 | Msa0687070:exon | 35.0% | |
| CTTTGATCTTCTTAAGAAGC+TGG | - | chr4_4:51754675-51754694 | Msa0687070:intron | 35.0% | |
| ACAAAAAGAGAGTCATGTGT+AGG | + | chr4_4:51754705-51754724 | None:intergenic | 35.0% | |
| !! | TTTCTAGACAGGTTTTGTGC+AGG | - | chr4_4:51754431-51754450 | Msa0687070:three_prime_UTR | 40.0% |
| !! | TTCTAGACAGGTTTTGTGCA+GGG | - | chr4_4:51754432-51754451 | Msa0687070:three_prime_UTR | 40.0% |
| CATCAAAGGCTACCATATGA+TGG | + | chr4_4:51754489-51754508 | None:intergenic | 40.0% | |
| TCATATGGTAGCCTTTGATG+TGG | - | chr4_4:51754489-51754508 | Msa0687070:three_prime_UTR | 40.0% | |
| !! | ACAAATCAGGTGCTATTAGC+TGG | - | chr4_4:51754758-51754777 | Msa0687070:intron | 40.0% |
| GCATTGTCTCTGCCATCATA+TGG | - | chr4_4:51754474-51754493 | Msa0687070:three_prime_UTR | 45.0% | |
| TGTGGCGCAACTCATGAAAT+TGG | - | chr4_4:51754507-51754526 | Msa0687070:three_prime_UTR | 45.0% | |
| AGATCAAAGTTGAGGTTGCG+TGG | + | chr4_4:51754664-51754683 | None:intergenic | 45.0% | |
| GATCTTCTTAAGAAGCTGGC+AGG | - | chr4_4:51754679-51754698 | Msa0687070:intron | 45.0% | |
| AACCTAGTCGTATCTCTCGT+CGG | + | chr4_4:51754731-51754750 | None:intergenic | 45.0% | |
| !! | TGCTATTAGCTGGTGTTGGT+TGG | - | chr4_4:51754768-51754787 | Msa0687070:intron | 45.0% |
| TCAAGGATCTTTAGATGCCC+TGG | + | chr4_4:51754829-51754848 | None:intergenic | 45.0% | |
| !! | CAGGTTTTGTGCAGGGATGA+CGG | - | chr4_4:51754439-51754458 | Msa0687070:three_prime_UTR | 50.0% |
| ! | TTTGTGCAGGGATGACGGTT+TGG | - | chr4_4:51754444-51754463 | Msa0687070:three_prime_UTR | 50.0% |
| ! | TTGTGCAGGGATGACGGTTT+GGG | - | chr4_4:51754445-51754464 | Msa0687070:three_prime_UTR | 50.0% |
| CATGAGTTGCGCCACATCAA+AGG | + | chr4_4:51754503-51754522 | None:intergenic | 50.0% | |
| GGCGCAACTCATGAAATTGG+TGG | - | chr4_4:51754510-51754529 | Msa0687070:three_prime_UTR | 50.0% | |
| ATCCGACGAGAGATACGACT+AGG | - | chr4_4:51754726-51754745 | Msa0687070:intron | 50.0% | |
| !! | CAGGTGCTATTAGCTGGTGT+TGG | - | chr4_4:51754764-51754783 | Msa0687070:intron | 50.0% |
| ! | TGTGCAGGGATGACGGTTTG+GGG | - | chr4_4:51754446-51754465 | Msa0687070:three_prime_UTR | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 51754389 | 51754930 | 51754389 | ID=Msa0687070;Name=Msa0687070 |
| chr4_4 | mRNA | 51754389 | 51754930 | 51754389 | ID=Msa0687070-mRNA-1;Parent=Msa0687070;Name=Msa0687070-mRNA-1;_AED=0.00;_eAED=0.00;_QI=11|1|1|1|0|0.5|2|218|69 |
| chr4_4 | exon | 51754389 | 51754665 | 51754389 | ID=Msa0687070-mRNA-1:exon:13454;Parent=Msa0687070-mRNA-1 |
| chr4_4 | exon | 51754769 | 51754930 | 51754769 | ID=Msa0687070-mRNA-1:exon:13453;Parent=Msa0687070-mRNA-1 |
| chr4_4 | five_prime_UTR | 51754920 | 51754930 | 51754920 | ID=Msa0687070-mRNA-1:five_prime_utr;Parent=Msa0687070-mRNA-1 |
| chr4_4 | CDS | 51754769 | 51754919 | 51754769 | ID=Msa0687070-mRNA-1:cds;Parent=Msa0687070-mRNA-1 |
| chr4_4 | CDS | 51754607 | 51754665 | 51754607 | ID=Msa0687070-mRNA-1:cds;Parent=Msa0687070-mRNA-1 |
| chr4_4 | three_prime_UTR | 51754389 | 51754606 | 51754389 | ID=Msa0687070-mRNA-1:three_prime_utr;Parent=Msa0687070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
