AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1342840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGCAGGGATGACGGTTT+GGG 0.226189 tig0006056:+79487 Msa1342840:CDS
TCATTATACCTAACATAAAT+TGG 0.230942 tig0006056:-79579 None:intergenic
CGTGTCAAGAGCGGGGTTCT+TGG 0.312982 tig0006056:+80515 Msa1342840:CDS
TTTGTGCAGGGATGACGGTT+TGG 0.330848 tig0006056:+79486 Msa1342840:CDS
GTGGATGTCCAATTTATGTT+AGG 0.355114 tig0006056:+79571 Msa1342840:CDS
TGTGGCGCAACTCATGAAAT+TGG 0.429611 tig0006056:+79549 Msa1342840:CDS
CAAGAAATCGTGTCAAGAGC+GGG 0.461491 tig0006056:+80507 Msa1342840:CDS
GCATTGTCTCTGCCATCATA+TGG 0.506035 tig0006056:+79516 Msa1342840:CDS
TGTGCAGGGATGACGGTTTG+GGG 0.521080 tig0006056:+79488 Msa1342840:CDS
CATGAGTTGCGCCACATCAA+AGG 0.541976 tig0006056:-79542 None:intergenic
CATCAAAGGCTACCATATGA+TGG 0.567903 tig0006056:-79528 None:intergenic
CTCGTATCACTCTTCACCCA+CGG 0.572581 tig0006056:-80542 None:intergenic
TTGTGTATAACAGTTCGTGA+TGG 0.600002 tig0006056:+80286 Msa1342840:intron
TGTATAACAGTTCGTGATGG+AGG 0.603492 tig0006056:+80289 Msa1342840:intron
GAGTGAACACGGTTGATGCT+TGG 0.608303 tig0006056:-80469 None:intergenic
CGGGGTTCTTGGAACACCGT+GGG 0.615525 tig0006056:+80526 Msa1342840:CDS
GGCGCAACTCATGAAATTGG+TGG 0.617859 tig0006056:+79552 Msa1342840:CDS
TCAAGAAATCGTGTCAAGAG+CGG 0.632895 tig0006056:+80506 Msa1342840:CDS
TCATATGGTAGCCTTTGATG+TGG 0.643558 tig0006056:+79531 Msa1342840:CDS
AAGAAATCGTGTCAAGAGCG+GGG 0.665713 tig0006056:+80508 Msa1342840:CDS
GCGGGGTTCTTGGAACACCG+TGG 0.682296 tig0006056:+80525 Msa1342840:CDS
CGTGGGTGAAGAGTGATACG+AGG 0.709427 tig0006056:+80543 Msa1342840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGTTTTAGTTCTATAAA+TGG + tig0006056:80013-80032 Msa1342840:intron 15.0%
!!! ATTTGTTTTAGTTCTATAAA+TGG + tig0006056:80013-80032 Msa1342840:intron 15.0%
!! TCATTATACCTAACATAAAT+TGG - tig0006056:79582-79601 None:intergenic 20.0%
!! TTTATACACATTCATGAAAT+GGG + tig0006056:79623-79642 Msa1342840:intron 20.0%
!!! TTTTGTTTTTATCTCTATGA+TGG + tig0006056:79665-79684 Msa1342840:intron 20.0%
!!! TTCATGTTTCTTCAATTTTA+TGG + tig0006056:80045-80064 Msa1342840:intron 20.0%
!!! AATAAACCTTTTTATGTCTA+TGG + tig0006056:80147-80166 Msa1342840:intron 20.0%
!! GAAGAATAAAAATAATGTAG+TGG + tig0006056:80565-80584 Msa1342840:CDS 20.0%
!! TCATTATACCTAACATAAAT+TGG - tig0006056:79582-79601 None:intergenic 20.0%
!! TTTATACACATTCATGAAAT+GGG + tig0006056:79623-79642 Msa1342840:intron 20.0%
!!! TTTTGTTTTTATCTCTATGA+TGG + tig0006056:79665-79684 Msa1342840:intron 20.0%
!!! TTCATGTTTCTTCAATTTTA+TGG + tig0006056:80045-80064 Msa1342840:intron 20.0%
!!! AATAAACCTTTTTATGTCTA+TGG + tig0006056:80147-80166 Msa1342840:intron 20.0%
!! GAAGAATAAAAATAATGTAG+TGG + tig0006056:80565-80584 Msa1342840:CDS 20.0%
!!! TTTTATTGTTGTTTCTAGAC+AGG + tig0006056:79462-79481 Msa1342840:CDS 25.0%
! CTTTATACACATTCATGAAA+TGG + tig0006056:79622-79641 Msa1342840:intron 25.0%
! ATTATTTGCTTAGAAACTCA+AGG - tig0006056:79888-79907 None:intergenic 25.0%
! TTGAGTTTCTAAGCAAATAA+TGG + tig0006056:79887-79906 Msa1342840:intron 25.0%
! TTGCTATGTACATAACTAAA+TGG + tig0006056:79962-79981 Msa1342840:intron 25.0%
!! CTTTAGCATAATTGTTTTCA+AGG - tig0006056:79988-80007 None:intergenic 25.0%
! TCAAATCCATAGACATAAAA+AGG - tig0006056:80156-80175 None:intergenic 25.0%
!!! GATTTTTTATAGTGGAAGTT+TGG + tig0006056:80256-80275 Msa1342840:intron 25.0%
! TACAATATAATACCAAGCAA+AGG + tig0006056:80394-80413 Msa1342840:intron 25.0%
!!! TTTTATTGTTGTTTCTAGAC+AGG + tig0006056:79462-79481 Msa1342840:CDS 25.0%
! CTTTATACACATTCATGAAA+TGG + tig0006056:79622-79641 Msa1342840:intron 25.0%
! ATTATTTGCTTAGAAACTCA+AGG - tig0006056:79888-79907 None:intergenic 25.0%
! TTGAGTTTCTAAGCAAATAA+TGG + tig0006056:79887-79906 Msa1342840:intron 25.0%
! TTGCTATGTACATAACTAAA+TGG + tig0006056:79962-79981 Msa1342840:intron 25.0%
!! CTTTAGCATAATTGTTTTCA+AGG - tig0006056:79988-80007 None:intergenic 25.0%
! TCAAATCCATAGACATAAAA+AGG - tig0006056:80156-80175 None:intergenic 25.0%
!!! GATTTTTTATAGTGGAAGTT+TGG + tig0006056:80256-80275 Msa1342840:intron 25.0%
! TACAATATAATACCAAGCAA+AGG + tig0006056:80394-80413 Msa1342840:intron 25.0%
!!! TATGTTTTGATGACTCTTAC+TGG + tig0006056:79433-79452 Msa1342840:exon 30.0%
TGAAATGGGCATAGATTTAA+TGG + tig0006056:79637-79656 Msa1342840:intron 30.0%
TCTTAAGAAGATCAAAGTTG+AGG - tig0006056:79714-79733 None:intergenic 30.0%
TAGGTTTATGCATACAAATC+AGG + tig0006056:79787-79806 Msa1342840:intron 30.0%
AGAAACTATCTAATGTTTCC+AGG + tig0006056:79850-79869 Msa1342840:intron 30.0%
GAAACTATCTAATGTTTCCA+GGG + tig0006056:79851-79870 Msa1342840:intron 30.0%
TGACAGACTAACATAAAGAA+TGG - tig0006056:79934-79953 None:intergenic 30.0%
TATGGATAACAACACATGAT+TGG + tig0006056:80063-80082 Msa1342840:intron 30.0%
ATGGATAACAACACATGATT+GGG + tig0006056:80064-80083 Msa1342840:intron 30.0%
TTCAAATTGCTATCTTCACA+TGG - tig0006056:80096-80115 None:intergenic 30.0%
GAACTACAATCTCATACAAA+GGG - tig0006056:80120-80139 None:intergenic 30.0%
TGAACTACAATCTCATACAA+AGG - tig0006056:80121-80140 None:intergenic 30.0%
!!! CAGTTCGTGATTTTTTATAG+TGG + tig0006056:80248-80267 Msa1342840:intron 30.0%
TCTACAATATTGTTGTAGCT+TGG + tig0006056:80365-80384 Msa1342840:intron 30.0%
CTACAATATTGTTGTAGCTT+GGG + tig0006056:80366-80385 Msa1342840:intron 30.0%
TATAATACCAAGCAAAGGTT+CGG + tig0006056:80399-80418 Msa1342840:intron 30.0%
!!! TATGTTTTGATGACTCTTAC+TGG + tig0006056:79433-79452 Msa1342840:exon 30.0%
TGAAATGGGCATAGATTTAA+TGG + tig0006056:79637-79656 Msa1342840:intron 30.0%
TCTTAAGAAGATCAAAGTTG+AGG - tig0006056:79714-79733 None:intergenic 30.0%
TAGGTTTATGCATACAAATC+AGG + tig0006056:79787-79806 Msa1342840:intron 30.0%
AGAAACTATCTAATGTTTCC+AGG + tig0006056:79850-79869 Msa1342840:intron 30.0%
GAAACTATCTAATGTTTCCA+GGG + tig0006056:79851-79870 Msa1342840:intron 30.0%
TGACAGACTAACATAAAGAA+TGG - tig0006056:79934-79953 None:intergenic 30.0%
TATGGATAACAACACATGAT+TGG + tig0006056:80063-80082 Msa1342840:intron 30.0%
ATGGATAACAACACATGATT+GGG + tig0006056:80064-80083 Msa1342840:intron 30.0%
TTCAAATTGCTATCTTCACA+TGG - tig0006056:80096-80115 None:intergenic 30.0%
GAACTACAATCTCATACAAA+GGG - tig0006056:80120-80139 None:intergenic 30.0%
TGAACTACAATCTCATACAA+AGG - tig0006056:80121-80140 None:intergenic 30.0%
!!! CAGTTCGTGATTTTTTATAG+TGG + tig0006056:80248-80267 Msa1342840:intron 30.0%
TCTACAATATTGTTGTAGCT+TGG + tig0006056:80365-80384 Msa1342840:intron 30.0%
CTACAATATTGTTGTAGCTT+GGG + tig0006056:80366-80385 Msa1342840:intron 30.0%
TATAATACCAAGCAAAGGTT+CGG + tig0006056:80399-80418 Msa1342840:intron 30.0%
GTGGATGTCCAATTTATGTT+AGG + tig0006056:79571-79590 Msa1342840:CDS 35.0%
AATGGGCATAGATTTAATGG+AGG + tig0006056:79640-79659 Msa1342840:intron 35.0%
CTTTGATCTTCTTAAGAAGC+TGG + tig0006056:79717-79736 Msa1342840:intron 35.0%
ACAAAAAGAGAGTCATGTGT+AGG - tig0006056:79747-79766 None:intergenic 35.0%
TTGTGTATAACAGTTCGTGA+TGG + tig0006056:80286-80305 Msa1342840:intron 35.0%
ATAATACCAAGCAAAGGTTC+GGG + tig0006056:80400-80419 Msa1342840:intron 35.0%
ATTTGACGATCTTCTTCATG+TGG - tig0006056:80429-80448 None:intergenic 35.0%
! GTCTTTTATTCGAGTGAACA+CGG - tig0006056:80483-80502 None:intergenic 35.0%
GTGGATGTCCAATTTATGTT+AGG + tig0006056:79571-79590 Msa1342840:CDS 35.0%
AATGGGCATAGATTTAATGG+AGG + tig0006056:79640-79659 Msa1342840:intron 35.0%
CTTTGATCTTCTTAAGAAGC+TGG + tig0006056:79717-79736 Msa1342840:intron 35.0%
ACAAAAAGAGAGTCATGTGT+AGG - tig0006056:79747-79766 None:intergenic 35.0%
TTGTGTATAACAGTTCGTGA+TGG + tig0006056:80286-80305 Msa1342840:intron 35.0%
ATAATACCAAGCAAAGGTTC+GGG + tig0006056:80400-80419 Msa1342840:intron 35.0%
ATTTGACGATCTTCTTCATG+TGG - tig0006056:80429-80448 None:intergenic 35.0%
! GTCTTTTATTCGAGTGAACA+CGG - tig0006056:80483-80502 None:intergenic 35.0%
!! TTTCTAGACAGGTTTTGTGC+AGG + tig0006056:79473-79492 Msa1342840:CDS 40.0%
!! TTCTAGACAGGTTTTGTGCA+GGG + tig0006056:79474-79493 Msa1342840:CDS 40.0%
CATCAAAGGCTACCATATGA+TGG - tig0006056:79531-79550 None:intergenic 40.0%
TCATATGGTAGCCTTTGATG+TGG + tig0006056:79531-79550 Msa1342840:CDS 40.0%
!! ACAAATCAGGTGCTATTAGC+TGG + tig0006056:79800-79819 Msa1342840:intron 40.0%
TGTATAACAGTTCGTGATGG+AGG + tig0006056:80289-80308 Msa1342840:intron 40.0%
! CGTGATGGAGGTAAAAAAGA+AGG + tig0006056:80301-80320 Msa1342840:CDS 40.0%
TCAAGAAATCGTGTCAAGAG+CGG + tig0006056:80506-80525 Msa1342840:CDS 40.0%
!! TTTCTAGACAGGTTTTGTGC+AGG + tig0006056:79473-79492 Msa1342840:CDS 40.0%
!! TTCTAGACAGGTTTTGTGCA+GGG + tig0006056:79474-79493 Msa1342840:CDS 40.0%
CATCAAAGGCTACCATATGA+TGG - tig0006056:79531-79550 None:intergenic 40.0%
TCATATGGTAGCCTTTGATG+TGG + tig0006056:79531-79550 Msa1342840:CDS 40.0%
!! ACAAATCAGGTGCTATTAGC+TGG + tig0006056:79800-79819 Msa1342840:intron 40.0%
TGTATAACAGTTCGTGATGG+AGG + tig0006056:80289-80308 Msa1342840:intron 40.0%
! CGTGATGGAGGTAAAAAAGA+AGG + tig0006056:80301-80320 Msa1342840:CDS 40.0%
TCAAGAAATCGTGTCAAGAG+CGG + tig0006056:80506-80525 Msa1342840:CDS 40.0%
GCATTGTCTCTGCCATCATA+TGG + tig0006056:79516-79535 Msa1342840:CDS 45.0%
TGTGGCGCAACTCATGAAAT+TGG + tig0006056:79549-79568 Msa1342840:CDS 45.0%
AGATCAAAGTTGAGGTTGCG+TGG - tig0006056:79706-79725 None:intergenic 45.0%
GATCTTCTTAAGAAGCTGGC+AGG + tig0006056:79721-79740 Msa1342840:intron 45.0%
ATCTGACGAGAGATACGACT+AGG + tig0006056:79768-79787 Msa1342840:intron 45.0%
!! TGCTATTAGCTGGTGTTGGT+TGG + tig0006056:79810-79829 Msa1342840:intron 45.0%
TCAAGGATCTTTAGATGCCC+TGG - tig0006056:79871-79890 None:intergenic 45.0%
CAAGAAATCGTGTCAAGAGC+GGG + tig0006056:80507-80526 Msa1342840:CDS 45.0%
AAGAAATCGTGTCAAGAGCG+GGG + tig0006056:80508-80527 Msa1342840:CDS 45.0%
GCATTGTCTCTGCCATCATA+TGG + tig0006056:79516-79535 Msa1342840:CDS 45.0%
TGTGGCGCAACTCATGAAAT+TGG + tig0006056:79549-79568 Msa1342840:CDS 45.0%
AGATCAAAGTTGAGGTTGCG+TGG - tig0006056:79706-79725 None:intergenic 45.0%
GATCTTCTTAAGAAGCTGGC+AGG + tig0006056:79721-79740 Msa1342840:intron 45.0%
ATCTGACGAGAGATACGACT+AGG + tig0006056:79768-79787 Msa1342840:intron 45.0%
!! TGCTATTAGCTGGTGTTGGT+TGG + tig0006056:79810-79829 Msa1342840:intron 45.0%
TCAAGGATCTTTAGATGCCC+TGG - tig0006056:79871-79890 None:intergenic 45.0%
CAAGAAATCGTGTCAAGAGC+GGG + tig0006056:80507-80526 Msa1342840:CDS 45.0%
AAGAAATCGTGTCAAGAGCG+GGG + tig0006056:80508-80527 Msa1342840:CDS 45.0%
!! CAGGTTTTGTGCAGGGATGA+CGG + tig0006056:79481-79500 Msa1342840:CDS 50.0%
! TTTGTGCAGGGATGACGGTT+TGG + tig0006056:79486-79505 Msa1342840:CDS 50.0%
! TTGTGCAGGGATGACGGTTT+GGG + tig0006056:79487-79506 Msa1342840:CDS 50.0%
CATGAGTTGCGCCACATCAA+AGG - tig0006056:79545-79564 None:intergenic 50.0%
GGCGCAACTCATGAAATTGG+TGG + tig0006056:79552-79571 Msa1342840:CDS 50.0%
!! CAGGTGCTATTAGCTGGTGT+TGG + tig0006056:79806-79825 Msa1342840:intron 50.0%
TGGATACCCGAACCTTTGCT+TGG - tig0006056:80409-80428 None:intergenic 50.0%
! GAGTGAACACGGTTGATGCT+TGG - tig0006056:80472-80491 None:intergenic 50.0%
CTCGTATCACTCTTCACCCA+CGG - tig0006056:80545-80564 None:intergenic 50.0%
!! CAGGTTTTGTGCAGGGATGA+CGG + tig0006056:79481-79500 Msa1342840:CDS 50.0%
! TTTGTGCAGGGATGACGGTT+TGG + tig0006056:79486-79505 Msa1342840:CDS 50.0%
! TTGTGCAGGGATGACGGTTT+GGG + tig0006056:79487-79506 Msa1342840:CDS 50.0%
CATGAGTTGCGCCACATCAA+AGG - tig0006056:79545-79564 None:intergenic 50.0%
GGCGCAACTCATGAAATTGG+TGG + tig0006056:79552-79571 Msa1342840:CDS 50.0%
!! CAGGTGCTATTAGCTGGTGT+TGG + tig0006056:79806-79825 Msa1342840:intron 50.0%
TGGATACCCGAACCTTTGCT+TGG - tig0006056:80409-80428 None:intergenic 50.0%
! GAGTGAACACGGTTGATGCT+TGG - tig0006056:80472-80491 None:intergenic 50.0%
CTCGTATCACTCTTCACCCA+CGG - tig0006056:80545-80564 None:intergenic 50.0%
! TGTGCAGGGATGACGGTTTG+GGG + tig0006056:79488-79507 Msa1342840:CDS 55.0%
CGTGGGTGAAGAGTGATACG+AGG + tig0006056:80543-80562 Msa1342840:CDS 55.0%
! TGTGCAGGGATGACGGTTTG+GGG + tig0006056:79488-79507 Msa1342840:CDS 55.0%
CGTGGGTGAAGAGTGATACG+AGG + tig0006056:80543-80562 Msa1342840:CDS 55.0%
CGTGTCAAGAGCGGGGTTCT+TGG + tig0006056:80515-80534 Msa1342840:CDS 60.0%
CGGGGTTCTTGGAACACCGT+GGG + tig0006056:80526-80545 Msa1342840:CDS 60.0%
CGTGTCAAGAGCGGGGTTCT+TGG + tig0006056:80515-80534 Msa1342840:CDS 60.0%
CGGGGTTCTTGGAACACCGT+GGG + tig0006056:80526-80545 Msa1342840:CDS 60.0%
GCGGGGTTCTTGGAACACCG+TGG + tig0006056:80525-80544 Msa1342840:CDS 65.0%
GCGGGGTTCTTGGAACACCG+TGG + tig0006056:80525-80544 Msa1342840:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0006056 gene 79431 80592 79431 ID=Msa1342840;Name=Msa1342840
tig0006056 mRNA 79431 80592 79431 ID=Msa1342840-mRNA-1;Parent=Msa1342840;Name=Msa1342840-mRNA-1;_AED=0.59;_eAED=0.59;_QI=11|0|0.33|0.33|1|1|3|0|114
tig0006056 exon 79431 79592 79431 ID=Msa1342840-mRNA-1:exon:3803;Parent=Msa1342840-mRNA-1
tig0006056 exon 80299 80335 80299 ID=Msa1342840-mRNA-1:exon:3804;Parent=Msa1342840-mRNA-1
tig0006056 exon 80436 80592 80436 ID=Msa1342840-mRNA-1:exon:3805;Parent=Msa1342840-mRNA-1
tig0006056 five_prime_UTR 79431 79441 79431 ID=Msa1342840-mRNA-1:five_prime_utr;Parent=Msa1342840-mRNA-1
tig0006056 CDS 79442 79592 79442 ID=Msa1342840-mRNA-1:cds;Parent=Msa1342840-mRNA-1
tig0006056 CDS 80299 80335 80299 ID=Msa1342840-mRNA-1:cds;Parent=Msa1342840-mRNA-1
tig0006056 CDS 80436 80592 80436 ID=Msa1342840-mRNA-1:cds;Parent=Msa1342840-mRNA-1
Gene Sequence

>Msa1342840

ATGACTCTTACTGGTTGTAGTTTTATTGTTGTTTCTAGACAGGTTTTGTGCAGGGATGACGGTTTGGGGCTGTTGCATTGTCTCTGCCATCATATGGTAGCCTTTGATGTGGCGCAACTCATGAAATTGGTGGATGTCCAATTTATGTTAGTTCGTGATGGAGGTAAAAAAGAAGGCTTAACTCATAAAAGATCGTCAAATGTATCAGATGATGAAGATCACCAAGCATCAACCGTGTTCACTCGAATAAAAGACAAGTCAAGAAATCGTGTCAAGAGCGGGGTTCTTGGAACACCGTGGGTGAAGAGTGATACGAGGAAGAATAAAAATAATGTAGTGGCGTAA

Protein sequence

>Msa1342840

MTLTGCSFIVVSRQVLCRDDGLGLLHCLCHHMVAFDVAQLMKLVDVQFMLVRDGGKKEGLTHKRSSNVSDDEDHQASTVFTRIKDKSRNRVKSGVLGTPWVKSDTRKNKNNVVA*