AlfalfaGEDB | Msa0744490

M. sativa cultivar zhongmu-4 / Msa0744490

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744490	AHG28987.1	64.674	368	66	5	1	304	966	1333	5.26e-139	441

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744490	W0LWR0	64.674	368	66	5	1	304	966	1333	2.51e-139	441

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0088550	Msa0744490	0.804832	1.787512e-49	-8.615850e-47
Msa0094940	Msa0744490	-0.804683	1.920001e-49	-8.615850e-47
Msa0135360	Msa0744490	0.801306	9.582210e-49	-8.615850e-47
Msa0153440	Msa0744490	0.825695	4.073684e-54	-8.615850e-47
Msa0185640	Msa0744490	-0.802224	6.208960e-49	-8.615850e-47
Msa0260270	Msa0744490	-0.803904	2.789858e-49	-8.615850e-47
Msa0264940	Msa0744490	-0.801291	9.651237e-49	-8.615850e-47
Msa0302990	Msa0744490	-0.802956	4.385958e-49	-8.615850e-47
Msa0342340	Msa0744490	-0.806699	7.244421e-50	-8.615850e-47
Msa0379340	Msa0744490	-0.809983	1.444663e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744490	MtrunA17_Chr5g0439121	46.348	356	133	9	1	301	1104	1456	2.25e-76	254
Msa0744490	MtrunA17_Chr5g0439121	30.084	359	182	13	2	305	796	1140	4.47e-20	90.9
Msa0744490	MtrunA17_Chr3g0108821	31.928	332	181	11	2	305	1056	1370	1.44e-31	124
Msa0744490	MtrunA17_Chr1g0160951	30.178	338	177	10	2	300	855	1172	1.89e-31	124
Msa0744490	MtrunA17_Chr3g0108811	30.793	328	182	11	2	301	1077	1387	1.81e-29	118
Msa0744490	MtrunA17_Chr5g0443761	31.461	356	177	11	4	300	678	1025	7.14e-26	107
Msa0744490	MtrunA17_Chr3g0108151	28.947	380	183	10	1	305	785	1152	1.61e-24	103
Msa0744490	MtrunA17_Chr3g0108071	30.189	265	154	7	56	305	270	518	2.16e-23	100
Msa0744490	MtrunA17_Chr3g0108111	27.645	293	166	9	51	305	45	329	3.01e-23	99.8
Msa0744490	MtrunA17_Chr3g0108011	25.526	333	185	8	2	305	1096	1394	3.39e-22	97.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 53 sgRNAs with CRISPR-Local

Find 353 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTATTTGAATGTCAAAA+AGG	0.379083	5_1:+65288793	Msa0744490:CDS
AAATAAAGATGAAATAATTC+AGG	0.389111	5_1:+65287409	Msa0744490:CDS
ACCCCACGAGTTTCCTGAAC+TGG	0.464861	5_1:+65288735	Msa0744490:CDS
ACGTACGGCAAGCAACTTCA+TGG	0.446669	5_1:+65288404	Msa0744490:CDS
ACTCCAGTTCAGGAAACTCG+TGG	0.601936	5_1:-65288738	None:intergenic
AGAGCTGACCTATATATGGA+AGG	0.590238	5_1:+65288456	Msa0744490:CDS
AGGATTGTCATGGAAGATGT+TGG	0.544244	5_1:+65287731	Msa0744490:CDS
ATATATCAACTCATGGTATT+CGG	0.396842	5_1:-65287360	None:intergenic
ATCAACTTCCAATGAACGTA+CGG	0.557403	5_1:+65288389	Msa0744490:CDS
ATCTTAAGTTGCTACACATT+TGG	0.280013	5_1:+65288506	Msa0744490:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTGTGAATTGCAACTTAT+TGG	+	chr5_1:65287487-65287506	Msa0744490:intron	30.0%
TTCCATGTATCTTATGTTCA+AGG	+	chr5_1:65287518-65287537	Msa0744490:intron	30.0%
GGGAAAATGTTGATCATATT+TGG	-	chr5_1:65287677-65287696	None:intergenic	30.0%
TTTGTCTGTTAATACTCTGA+TGG	+	chr5_1:65287763-65287782	Msa0744490:CDS	30.0%
CAAGACAACATTTCTATTTG+GGG	-	chr5_1:65287906-65287925	None:intergenic	30.0%
CCAAGACAACATTTCTATTT+GGG	-	chr5_1:65287907-65287926	None:intergenic	30.0%
TCCAAGACAACATTTCTATT+TGG	-	chr5_1:65287908-65287927	None:intergenic	30.0%
GCATTGTCCTAATAATAATG+GGG	+	chr5_1:65288031-65288050	Msa0744490:intron	30.0%
TTAGGAACCCCATTATTATT+AGG	-	chr5_1:65288041-65288060	None:intergenic	30.0%
TAATTAAATTGGGCACCTAA+TGG	+	chr5_1:65288095-65288114	Msa0744490:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	65287293	65292885	65287293	ID=Msa0744490;Name=Msa0744490
chr5_1	mRNA	65287293	65292885	65287293	ID=Msa0744490-mRNA-1;Parent=Msa0744490;Name=Msa0744490-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0\|0\|0\|0.8\|0.25\|0\|5\|0\|308
chr5_1	exon	65287293	65287479	65287293	ID=Msa0744490-mRNA-1:exon:19411;Parent=Msa0744490-mRNA-1
chr5_1	exon	65287540	65287784	65287540	ID=Msa0744490-mRNA-1:exon:19412;Parent=Msa0744490-mRNA-1
chr5_1	exon	65288298	65288529	65288298	ID=Msa0744490-mRNA-1:exon:19413;Parent=Msa0744490-mRNA-1
chr5_1	exon	65288566	65288814	65288566	ID=Msa0744490-mRNA-1:exon:19414;Parent=Msa0744490-mRNA-1
chr5_1	exon	65292872	65292885	65292872	ID=Msa0744490-mRNA-1:exon:19415;Parent=Msa0744490-mRNA-1
chr5_1	CDS	65287293	65287479	65287293	ID=Msa0744490-mRNA-1:cds;Parent=Msa0744490-mRNA-1
chr5_1	CDS	65287540	65287784	65287540	ID=Msa0744490-mRNA-1:cds;Parent=Msa0744490-mRNA-1
chr5_1	CDS	65288298	65288529	65288298	ID=Msa0744490-mRNA-1:cds;Parent=Msa0744490-mRNA-1
chr5_1	CDS	65288566	65288814	65288566	ID=Msa0744490-mRNA-1:cds;Parent=Msa0744490-mRNA-1
chr5_1	CDS	65292872	65292885	65292872	ID=Msa0744490-mRNA-1:cds;Parent=Msa0744490-mRNA-1

Gene Sequence

>Msa0744490

ATGTTAAGAACATTGTTTTCACCATCCACAGCTATGCACTTGACTTCATTGCAAGAATTGATGATAGCCGAATACCATGAGTTGATATATTTAGTAAGTTATGGAAAATCACATGAAAATAAAGATGAAATAATTCAGGAAGATCATGATTCGCAGAGTTACGATGCAATATTCCCAAGATTGAAAAGCCTGGTGAATCTTAGGGATATTGAAATACGGCAAACACATAGTTTGATATATGTATTTGGTCATGGTTTGATGTACCATAACAAGATCCAAACTGAGCTTCCTGTTTTAGAAAAAGTTGCACTCTTCAGTATACCAAATATGATCAACATTTTCCCAGAAAATTACAATATAAATTGCCCATCTTTGCTAAGGATTGTCATGGAAGATGTTGGACTATCCACTTTGTCTGTTAATACTCTGATGGATATAGTGGAAGCAAGTATAAAAAATATGCAGGTGTTGGAATCTGTTGCTGTTTGGAACAGCTCTAAAATAAAAGGCATTTTTCACCAAGATCAACTTCCAATGAACGTACGGCAAGCAACTTCATGGTTAATAGACCTGAACTTGGTCAATTTACCAGAGCTGACCTATATATGGAAGGGTGCCAAACATTCTACGACCCTTCAAAATCTTAAGTTGCTACACATTTGGGTCGCTAGAAGCCTACCTCAGTTGAAGATCCTAGTCGCATTACAGTGTGATGGATTGGAGCAAATTATTGAAGATGATGAAGAGCATGAAAATGCAATATACTTCTCAAAATTAAAGTTGATTCTTGTCACACAATGCAATAACTTAAAGCGCCTGTTCTATTTCTCTGCACCCCACGAGTTTCCTGAACTGGAGTATTTGATCATAAATCAAGATCCTAATCTGGAGAAAGTATTTGAATGTCAAAAAGACAGTATACTTTAG

Protein sequence

>Msa0744490

MLRTLFSPSTAMHLTSLQELMIAEYHELIYLVSYGKSHENKDEIIQEDHDSQSYDAIFPRLKSLVNLRDIEIRQTHSLIYVFGHGLMYHNKIQTELPVLEKVALFSIPNMINIFPENYNINCPSLLRIVMEDVGLSTLSVNTLMDIVEASIKNMQVLESVAVWNSSKIKGIFHQDQLPMNVRQATSWLIDLNLVNLPELTYIWKGAKHSTTLQNLKLLHIWVARSLPQLKILVALQCDGLEQIIEDDEEHENAIYFSKLKLILVTQCNNLKRLFYFSAPHEFPELEYLIINQDPNLEKVFECQKDSIL*