AlfalfaGEDB | Msa0932840

M. sativa cultivar zhongmu-4 / Msa0932840

KEGG: K17681
GO: -
PFAM: PF00004.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0932840	sp\|Q6PAX2\|ATD3B_XENLA	46.078	204	101	1	1	204	372	566	9.93e-53	181

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0932840	A0A072UGA9	97.630	211	5	0	1	211	266	476	1.88e-144	420

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011960	Msa0932840	0.800958	1.129102e-48	-8.615850e-47
Msa0043660	Msa0932840	0.808911	2.454168e-50	-8.615850e-47
Msa0067630	Msa0932840	0.800544	1.371276e-48	-8.615850e-47
Msa0137550	Msa0932840	0.817212	3.707437e-52	-8.615850e-47
Msa0159170	Msa0932840	0.835258	1.862707e-56	-8.615850e-47
Msa0220720	Msa0932840	0.803336	3.660332e-49	-8.615850e-47
Msa0228070	Msa0932840	0.809615	1.733992e-50	-8.615850e-47
Msa0299870	Msa0932840	0.802481	5.497375e-49	-8.615850e-47
Msa0325590	Msa0932840	0.814229	1.713151e-51	-8.615850e-47
Msa0337240	Msa0932840	0.810367	1.193785e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0932840	MtrunA17_Chr6g0450161	97.630	211	5	0	1	211	394	604	1.35e-147	423
Msa0932840	MtrunA17_Chr4g0039781	87.204	211	26	1	1	210	400	610	1.44e-130	380
Msa0932840	MtrunA17_Chr5g0404291	77.404	208	44	2	1	207	419	624	1.18e-104	314

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0932840	AT3G03060.1	84.762	210	31	1	1	209	417	626	2.84e-126	370
Msa0932840	AT5G16930.1	84.286	210	32	1	1	209	429	638	6.45e-126	370
Msa0932840	AT2G18330.1	76.526	213	43	4	1	206	416	628	4.09e-102	308
Msa0932840	AT4G36580.1	72.512	211	54	2	1	207	413	623	3.65e-96	293

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Find 68 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATGATAAAAGCATGCT+CGG	0.514020	6_3:+1131534	None:intergenic
AAGGGAAATAGCGAAACTGA+TGG	0.642775	6_3:-1131757	Msa0932840:CDS
AATGCCCTTCTCTTTCGTAC+TGG	0.505763	6_3:-1132065	Msa0932840:CDS
ACAGCTGAATCAAGGTCACC+AGG	0.637144	6_3:+1131999	None:intergenic
ACAGTTGTCGCTTCCATACA+CGG	0.583469	6_3:+1131719	None:intergenic
ACCAGGACGGTTCGTCGCAA+GGG	0.541165	6_3:+1132016	None:intergenic
ACCATGAATTATCGTGGTTA+AGG	0.365020	6_3:+1131365	None:intergenic
ACCCTTGCGACGAACCGTCC+TGG	0.519465	6_3:-1132017	Msa0932840:CDS
ACGATAATTCATGGTGATTG+AGG	0.478951	6_3:-1131357	Msa0932840:three_prime_UTR
ACGCTCCTCTTCCCCAGGCA+AGG	0.527101	6_3:+1131944	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAGAATTGTCTGAGAAAAT+TGG	-	chr6_3:1131596-1131615	Msa0932840:three_prime_UTR	30.0%
GAGAATTGTCTGAGAAAATT+GGG	-	chr6_3:1131597-1131616	Msa0932840:three_prime_UTR	30.0%
TAAGGACTATGTCTTTAGAT+TGG	+	chr6_3:1131704-1131723	None:intergenic	30.0%
CCACAAAAGATAGAGATTAA+AGG	-	chr6_3:1131906-1131925	Msa0932840:CDS	30.0%
ATTGAGCGATGATATCATAA+AGG	-	chr6_3:1131929-1131948	Msa0932840:CDS	30.0%
ATTGTTTGTTCCTGATTGAT+GGG	-	chr6_3:1132223-1132242	Msa0932840:intron	30.0%
TCAAGTGTTACATAAAGTCA+TGG	+	chr6_3:1132314-1132333	None:intergenic	30.0%
CTTTATGTAACACTTGATTC+AGG	-	chr6_3:1132317-1132336	Msa0932840:intron	30.0%
TGAGAATTGTCTGAGAAAAT+TGG	-	chr6_3:1131596-1131615	Msa0932840:three_prime_UTR	30.0%
GAGAATTGTCTGAGAAAATT+GGG	-	chr6_3:1131597-1131616	Msa0932840:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	1131295	1132467	1131295	ID=Msa0932840;Name=Msa0932840
chr6_3	mRNA	1131295	1132467	1131295	ID=Msa0932840-mRNA-1;Parent=Msa0932840;Name=Msa0932840-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|1\|0.5\|1\|1\|1\|2\|331\|211
chr6_3	exon	1131295	1132130	1131295	ID=Msa0932840-mRNA-1:exon:463;Parent=Msa0932840-mRNA-1
chr6_3	exon	1132337	1132467	1132337	ID=Msa0932840-mRNA-1:exon:462;Parent=Msa0932840-mRNA-1
chr6_3	CDS	1132337	1132467	1132337	ID=Msa0932840-mRNA-1:cds;Parent=Msa0932840-mRNA-1
chr6_3	CDS	1131626	1132130	1131626	ID=Msa0932840-mRNA-1:cds;Parent=Msa0932840-mRNA-1
chr6_3	three_prime_UTR	1131295	1131625	1131295	ID=Msa0932840-mRNA-1:three_prime_utr;Parent=Msa0932840-mRNA-1

Gene Sequence

>Msa0932840

ATGACTGGAGGAGATGTTGCTCCACTCGGATCACAGGCTGTAACAAAGATACACGAGTTGTTTGATTGGGCCAAGAAGTCCAATAGGGGTTTACTGCTTTTTATTGACGAAGCTGATGCATTTCTATGCGAGCGGAACAAGACGTATATGAGTGAAGCACAAAGAAGTGCACTAAATGCCCTTCTCTTTCGTACTGGTGACCAATCTAAAGACATAGTCCTTACCCTTGCGACGAACCGTCCTGGTGACCTTGATTCAGCTGTAGCAGACCGTATTGATGAGGTCCTAGAATTTCCCTTGCCTGGGGAAGAGGAGCGTTTTAAACTTCTTAAGCTCTACCTAGACAAGTACATAGCTCAAGCTGGATCAAGAAAATCTGGTTTATTATCATTGTTCAAAGCAAGCCCACAAAAGATAGAGATTAAAGGATTGAGCGATGATATCATAAAGGAAGCCGCAGCTAAAACCGATGGATTTTCCGGAAGGGAAATAGCGAAACTGATGGCAAGTGTCCAAGCTGCCGTGTATGGAAGCGACAACTGTATCCTTGACGCAAGCTTGTTCCGTGAAGTCGTAGATTATAAGGTTGCTGAGCATCAACAAAGAATAAAATTGGCAGCTTCTGATAAAGCTTAG

Protein sequence

>Msa0932840

MTGGDVAPLGSQAVTKIHELFDWAKKSNRGLLLFIDEADAFLCERNKTYMSEAQRSALNALLFRTGDQSKDIVLTLATNRPGDLDSAVADRIDEVLEFPLPGEEERFKLLKLYLDKYIAQAGSRKSGLLSLFKASPQKIEIKGLSDDIIKEAAAKTDGFSGREIAKLMASVQAAVYGSDNCILDASLFREVVDYKVAEHQQRIKLAASDKA*