AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0991080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0991080 MtrunA17_Chr1g0187021 88.660 97 3 2 14 103 344 439 6.36e-54 177
Msa0991080 MtrunA17_Chr1g0187071 69.000 100 23 2 11 103 340 438 1.05e-38 136
Msa0991080 MtrunA17_Chr1g0187051 68.041 97 13 2 14 103 339 424 4.22e-37 132
Msa0991080 MtrunA17_Chr7g0260781 55.000 100 31 2 11 103 202 294 9.22e-28 105
Msa0991080 MtrunA17_Chr1g0184551 61.628 86 24 2 25 103 347 430 3.17e-27 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0991080 AT1G04280.2 71.429 77 15 1 34 103 323 399 2.07e-31 117
Msa0991080 AT1G04280.1 71.429 77 15 1 34 103 377 453 3.36e-31 116
Msa0991080 AT1G06750.3 59.794 97 25 3 14 103 342 431 3.32e-29 111
Msa0991080 AT1G06750.1 59.794 97 25 3 14 103 342 431 3.32e-29 111
Msa0991080 AT1G06750.2 59.794 97 25 3 14 103 349 438 3.51e-29 111
Msa0991080 AT2G30630.1 59.794 97 25 3 14 103 356 445 1.23e-28 109
Msa0991080 AT2G30630.2 59.794 97 25 3 14 103 377 466 1.42e-28 109

Find 26 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACAAGTTTGCAATATTT+TGG 0.119289 6_4:-54553834 None:intergenic
CTTGTAGCTCTGCTTCTGTT+AGG 0.288048 6_4:-54554539 None:intergenic
GGATATCTTGCAGTTATTAG+AGG 0.406489 6_4:+54553629 Msa0991080:CDS
TTGCAGTTATTAGAGGCATT+AGG 0.422529 6_4:+54553636 Msa0991080:CDS
TTGTAGCTCTGCTTCTGTTA+GGG 0.423721 6_4:-54554538 None:intergenic
CAACAAGAGGAAAATTCTAC+AGG 0.441415 6_4:+54553551 Msa0991080:CDS
CTCTATTGAATCTGCAGAAG+CGG 0.490130 6_4:+54554508 Msa0991080:CDS
CGCTTCTGCAGATTCAATAG+AGG 0.492291 6_4:-54554507 None:intergenic
GCAAACTTGTTGACAGTGCT+AGG 0.511680 6_4:+54553844 Msa0991080:CDS
CTAGTTGGTGTGGTTTGTGA+TGG 0.513280 6_4:+54553608 Msa0991080:CDS
CTATACAAAGCAAGTGAATG+AGG 0.513355 6_4:+54553514 Msa0991080:CDS
CCAACTAGCTCAATTCTGTA+AGG 0.525410 6_4:-54553593 None:intergenic
CCTTACAGAATTGAGCTAGT+TGG 0.526663 6_4:+54553593 Msa0991080:CDS
CAGAATTGAGCTAGTTGGTG+TGG 0.527716 6_4:+54553598 Msa0991080:CDS
GGAAGAGAAGAGTTGTTAGC+TGG 0.534658 6_4:+54554581 Msa0991080:CDS
TGCTGTTGGTGGTCCACCAA+AGG 0.575982 6_4:+54553881 Msa0991080:CDS
AATGACATGAAGGGCAGTGA+GGG 0.577678 6_4:+54553767 Msa0991080:intron
GTTGTTAGCTGGTAATGAAG+AGG 0.582848 6_4:+54554592 Msa0991080:CDS
CGCTATTGCACAGATGCTGT+TGG 0.590494 6_4:+54553867 Msa0991080:CDS
TATTGCACAGATGCTGTTGG+TGG 0.593752 6_4:+54553870 Msa0991080:CDS
AGAAGAAGAACATCAACAAG+AGG 0.597156 6_4:+54553538 Msa0991080:CDS
AACAAGAGGAAAATTCTACA+GGG 0.606029 6_4:+54553552 Msa0991080:CDS
TATACAAAGCAAGTGAATGA+GGG 0.610525 6_4:+54553515 Msa0991080:CDS
AGTACTGCTTCAAGAAGTCG+AGG 0.646449 6_4:+54554560 Msa0991080:CDS
TAATGACATGAAGGGCAGTG+AGG 0.672210 6_4:+54553766 Msa0991080:intron
ACAAGAGGAAAATTCTACAG+GGG 0.709176 6_4:+54553553 Msa0991080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTACTATATTAATATACTT+TGG + chr6_4:54553677-54553696 Msa0991080:intron 15.0%
!!! TATAATTAACATTTTCCTTT+TGG + chr6_4:54553724-54553743 Msa0991080:intron 15.0%
!!! CTTTTCATTATTGATTTATT+GGG + chr6_4:54554163-54554182 Msa0991080:intron 15.0%
!!! TTTTCATTATTGATTTATTG+GGG + chr6_4:54554164-54554183 Msa0991080:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG - chr6_4:54554238-54554257 None:intergenic 15.0%
!! TCTACTATATTAATATACTT+TGG + chr6_4:54553677-54553696 Msa0991080:intron 15.0%
!!! TATAATTAACATTTTCCTTT+TGG + chr6_4:54553724-54553743 Msa0991080:intron 15.0%
!!! CTTTTCATTATTGATTTATT+GGG + chr6_4:54554163-54554182 Msa0991080:intron 15.0%
!!! TTTTCATTATTGATTTATTG+GGG + chr6_4:54554164-54554183 Msa0991080:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG - chr6_4:54554238-54554257 None:intergenic 15.0%
!! ACTATATTAATATACTTTGG+TGG + chr6_4:54553680-54553699 Msa0991080:intron 20.0%
!!! TTGTACATTTTTATTTCACA+TGG + chr6_4:54554058-54554077 Msa0991080:intron 20.0%
!! CCAATAAATCAATAATGAAA+AGG - chr6_4:54554165-54554184 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAT+TGG + chr6_4:54554162-54554181 Msa0991080:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG - chr6_4:54554234-54554253 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG - chr6_4:54554494-54554513 None:intergenic 20.0%
!! ACTATATTAATATACTTTGG+TGG + chr6_4:54553680-54553699 Msa0991080:intron 20.0%
!!! TTGTACATTTTTATTTCACA+TGG + chr6_4:54554058-54554077 Msa0991080:intron 20.0%
!! CCAATAAATCAATAATGAAA+AGG - chr6_4:54554165-54554184 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAT+TGG + chr6_4:54554162-54554181 Msa0991080:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG - chr6_4:54554234-54554253 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG - chr6_4:54554494-54554513 None:intergenic 20.0%
!!! ATACATGCATTTTTTGTGAA+AGG + chr6_4:54553488-54553507 Msa0991080:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG + chr6_4:54553736-54553755 Msa0991080:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG - chr6_4:54553837-54553856 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG - chr6_4:54554028-54554047 None:intergenic 25.0%
! CTTATTAACTCAGATATCAA+AGG - chr6_4:54554263-54554282 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG + chr6_4:54554397-54554416 Msa0991080:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG + chr6_4:54554425-54554444 Msa0991080:intron 25.0%
!!! ATACATGCATTTTTTGTGAA+AGG + chr6_4:54553488-54553507 Msa0991080:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG + chr6_4:54553736-54553755 Msa0991080:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG - chr6_4:54553837-54553856 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG - chr6_4:54554028-54554047 None:intergenic 25.0%
! CTTATTAACTCAGATATCAA+AGG - chr6_4:54554263-54554282 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG + chr6_4:54554397-54554416 Msa0991080:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG + chr6_4:54554425-54554444 Msa0991080:intron 25.0%
TATACAAAGCAAGTGAATGA+GGG + chr6_4:54553515-54553534 Msa0991080:CDS 30.0%
AACAAGAGGAAAATTCTACA+GGG + chr6_4:54553552-54553571 Msa0991080:CDS 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG + chr6_4:54553701-54553720 Msa0991080:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG - chr6_4:54553742-54553761 None:intergenic 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG + chr6_4:54553987-54554006 Msa0991080:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG - chr6_4:54554096-54554115 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG + chr6_4:54554424-54554443 Msa0991080:intron 30.0%
TATACAAAGCAAGTGAATGA+GGG + chr6_4:54553515-54553534 Msa0991080:CDS 30.0%
AACAAGAGGAAAATTCTACA+GGG + chr6_4:54553552-54553571 Msa0991080:CDS 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG + chr6_4:54553701-54553720 Msa0991080:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG - chr6_4:54553742-54553761 None:intergenic 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG + chr6_4:54553987-54554006 Msa0991080:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG - chr6_4:54554096-54554115 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG + chr6_4:54554424-54554443 Msa0991080:intron 30.0%
CTATACAAAGCAAGTGAATG+AGG + chr6_4:54553514-54553533 Msa0991080:CDS 35.0%
AGAAGAAGAACATCAACAAG+AGG + chr6_4:54553538-54553557 Msa0991080:CDS 35.0%
CAACAAGAGGAAAATTCTAC+AGG + chr6_4:54553551-54553570 Msa0991080:CDS 35.0%
ACAAGAGGAAAATTCTACAG+GGG + chr6_4:54553553-54553572 Msa0991080:CDS 35.0%
GGATATCTTGCAGTTATTAG+AGG + chr6_4:54553629-54553648 Msa0991080:CDS 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG + chr6_4:54553636-54553655 Msa0991080:CDS 35.0%
GAGAGCTATAATGACATGAA+GGG + chr6_4:54553758-54553777 Msa0991080:intron 35.0%
! TCTTTGGCCAAAACGATTTT+AGG - chr6_4:54553997-54554016 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG + chr6_4:54554184-54554203 Msa0991080:intron 35.0%
ACTTCAGTCATCTATACCTT+CGG - chr6_4:54554416-54554435 None:intergenic 35.0%
CTATACAAAGCAAGTGAATG+AGG + chr6_4:54553514-54553533 Msa0991080:CDS 35.0%
AGAAGAAGAACATCAACAAG+AGG + chr6_4:54553538-54553557 Msa0991080:CDS 35.0%
CAACAAGAGGAAAATTCTAC+AGG + chr6_4:54553551-54553570 Msa0991080:CDS 35.0%
ACAAGAGGAAAATTCTACAG+GGG + chr6_4:54553553-54553572 Msa0991080:CDS 35.0%
GGATATCTTGCAGTTATTAG+AGG + chr6_4:54553629-54553648 Msa0991080:CDS 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG + chr6_4:54553636-54553655 Msa0991080:CDS 35.0%
GAGAGCTATAATGACATGAA+GGG + chr6_4:54553758-54553777 Msa0991080:intron 35.0%
! TCTTTGGCCAAAACGATTTT+AGG - chr6_4:54553997-54554016 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG + chr6_4:54554184-54554203 Msa0991080:intron 35.0%
ACTTCAGTCATCTATACCTT+CGG - chr6_4:54554416-54554435 None:intergenic 35.0%
CCAACTAGCTCAATTCTGTA+AGG - chr6_4:54553596-54553615 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG + chr6_4:54553593-54553612 Msa0991080:CDS 40.0%
GGAGAGCTATAATGACATGA+AGG + chr6_4:54553757-54553776 Msa0991080:intron 40.0%
!!! GTTGCTCCTGTTTTTACCTT+TGG - chr6_4:54553900-54553919 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG + chr6_4:54554002-54554021 Msa0991080:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG - chr6_4:54554013-54554032 None:intergenic 40.0%
GAATGTTTAACAGCCTCTCA+GGG - chr6_4:54554355-54554374 None:intergenic 40.0%
TGAATGTTTAACAGCCTCTC+AGG - chr6_4:54554356-54554375 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG + chr6_4:54554508-54554527 Msa0991080:CDS 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG - chr6_4:54554541-54554560 None:intergenic 40.0%
CCAACTAGCTCAATTCTGTA+AGG - chr6_4:54553596-54553615 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG + chr6_4:54553593-54553612 Msa0991080:CDS 40.0%
GGAGAGCTATAATGACATGA+AGG + chr6_4:54553757-54553776 Msa0991080:intron 40.0%
!!! GTTGCTCCTGTTTTTACCTT+TGG - chr6_4:54553900-54553919 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG + chr6_4:54554002-54554021 Msa0991080:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG - chr6_4:54554013-54554032 None:intergenic 40.0%
GAATGTTTAACAGCCTCTCA+GGG - chr6_4:54554355-54554374 None:intergenic 40.0%
TGAATGTTTAACAGCCTCTC+AGG - chr6_4:54554356-54554375 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG + chr6_4:54554508-54554527 Msa0991080:CDS 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG - chr6_4:54554541-54554560 None:intergenic 40.0%
! CAGAATTGAGCTAGTTGGTG+TGG + chr6_4:54553598-54553617 Msa0991080:CDS 45.0%
! CTAGTTGGTGTGGTTTGTGA+TGG + chr6_4:54553608-54553627 Msa0991080:CDS 45.0%
TAATGACATGAAGGGCAGTG+AGG + chr6_4:54553766-54553785 Msa0991080:intron 45.0%
AATGACATGAAGGGCAGTGA+GGG + chr6_4:54553767-54553786 Msa0991080:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG + chr6_4:54553844-54553863 Msa0991080:CDS 45.0%
TATTGCACAGATGCTGTTGG+TGG + chr6_4:54553870-54553889 Msa0991080:CDS 45.0%
GGTCCACCAAAGGTAAAAAC+AGG + chr6_4:54553891-54553910 Msa0991080:intron 45.0%
!!! GCTCCTGTTTTTACCTTTGG+TGG - chr6_4:54553897-54553916 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG + chr6_4:54554436-54554455 Msa0991080:intron 45.0%
CGCTTCTGCAGATTCAATAG+AGG - chr6_4:54554510-54554529 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG - chr6_4:54554542-54554561 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG + chr6_4:54554560-54554579 Msa0991080:CDS 45.0%
GGAAGAGAAGAGTTGTTAGC+TGG + chr6_4:54554581-54554600 Msa0991080:CDS 45.0%
! CAGAATTGAGCTAGTTGGTG+TGG + chr6_4:54553598-54553617 Msa0991080:CDS 45.0%
! CTAGTTGGTGTGGTTTGTGA+TGG + chr6_4:54553608-54553627 Msa0991080:CDS 45.0%
TAATGACATGAAGGGCAGTG+AGG + chr6_4:54553766-54553785 Msa0991080:intron 45.0%
AATGACATGAAGGGCAGTGA+GGG + chr6_4:54553767-54553786 Msa0991080:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG + chr6_4:54553844-54553863 Msa0991080:CDS 45.0%
TATTGCACAGATGCTGTTGG+TGG + chr6_4:54553870-54553889 Msa0991080:CDS 45.0%
GGTCCACCAAAGGTAAAAAC+AGG + chr6_4:54553891-54553910 Msa0991080:intron 45.0%
!!! GCTCCTGTTTTTACCTTTGG+TGG - chr6_4:54553897-54553916 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG + chr6_4:54554436-54554455 Msa0991080:intron 45.0%
CGCTTCTGCAGATTCAATAG+AGG - chr6_4:54554510-54554529 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG - chr6_4:54554542-54554561 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG + chr6_4:54554560-54554579 Msa0991080:CDS 45.0%
GGAAGAGAAGAGTTGTTAGC+TGG + chr6_4:54554581-54554600 Msa0991080:CDS 45.0%
CGCTATTGCACAGATGCTGT+TGG + chr6_4:54553867-54553886 Msa0991080:CDS 50.0%
CACCATGTATCAACCCTGAG+AGG + chr6_4:54554339-54554358 Msa0991080:intron 50.0%
!! AGCCTCTCAGGGTTGATACA+TGG - chr6_4:54554344-54554363 None:intergenic 50.0%
CGCTATTGCACAGATGCTGT+TGG + chr6_4:54553867-54553886 Msa0991080:CDS 50.0%
CACCATGTATCAACCCTGAG+AGG + chr6_4:54554339-54554358 Msa0991080:intron 50.0%
!! AGCCTCTCAGGGTTGATACA+TGG - chr6_4:54554344-54554363 None:intergenic 50.0%
!! TGCTGTTGGTGGTCCACCAA+AGG + chr6_4:54553881-54553900 Msa0991080:CDS 55.0%
!! TGCTGTTGGTGGTCCACCAA+AGG + chr6_4:54553881-54553900 Msa0991080:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 54553473 54554613 54553473 ID=Msa0991080;Name=Msa0991080
chr6_4 mRNA 54553473 54554613 54553473 ID=Msa0991080-mRNA-1;Parent=Msa0991080;Name=Msa0991080-mRNA-1;_AED=0.22;_eAED=0.55;_QI=0|0|0|0.66|1|1|3|0|142
chr6_4 exon 54553473 54553657 54553473 ID=Msa0991080-mRNA-1:exon:11187;Parent=Msa0991080-mRNA-1
chr6_4 exon 54553779 54553902 54553779 ID=Msa0991080-mRNA-1:exon:11188;Parent=Msa0991080-mRNA-1
chr6_4 exon 54554497 54554613 54554497 ID=Msa0991080-mRNA-1:exon:11189;Parent=Msa0991080-mRNA-1
chr6_4 CDS 54553473 54553657 54553473 ID=Msa0991080-mRNA-1:cds;Parent=Msa0991080-mRNA-1
chr6_4 CDS 54553779 54553902 54553779 ID=Msa0991080-mRNA-1:cds;Parent=Msa0991080-mRNA-1
chr6_4 CDS 54554497 54554613 54554497 ID=Msa0991080-mRNA-1:cds;Parent=Msa0991080-mRNA-1
Gene Sequence

>Msa0991080

ATGATTATTCAAACTATACATGCATTTTTTGTGAAAGGAAACTATACAAAGCAAGTGAATGAGGGAGAAGAAGAACATCAACAAGAGGAAAATTCTACAGGGGAACTACACTCTCAAAAGCCTTACAGAATTGAGCTAGTTGGTGTGGTTTGTGATGGATATCTTGCAGTTATTAGAGGCATTAGGGCAGTGAGGGTGAATTCACAATTGCAATCTCACAAAAGATTTGCTAATGCATTTCCAAAATATTGCAAACTTGTTGACAGTGCTAGGCGCTATTGCACAGATGCTGTTGGTGGTCCACCAAAGAATAATTTTACCTCTATTGAATCTGCAGAAGCGGTTAGAGTCCCTAACAGAAGCAGAGCTACAAGTACTGCTTCAAGAAGTCGAGGAAGAGAAGAGTTGTTAGCTGGTAATGAAGAG

Protein sequence

>Msa0991080

MIIQTIHAFFVKGNYTKQVNEGEEEHQQEENSTGELHSQKPYRIELVGVVCDGYLAVIRGIRAVRVNSQLQSHKRFANAFPKYCKLVDSARRYCTDAVGGPPKNNFTSIESAEAVRVPNRSRATSTASRSRGREELLAGNEE