AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0918700


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0918700 MtrunA17_Chr1g0187021 88.660 97 3 2 14 103 344 439 1.77e-54 177
Msa0918700 MtrunA17_Chr1g0187071 71.134 97 20 2 14 103 343 438 3.66e-39 137
Msa0918700 MtrunA17_Chr1g0187051 68.041 97 13 2 14 103 339 424 6.63e-38 134
Msa0918700 MtrunA17_Chr7g0260781 69.444 72 15 1 39 103 223 294 1.48e-28 107
Msa0918700 MtrunA17_Chr1g0184551 61.628 86 24 2 25 103 347 430 5.59e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0918700 AT1G04280.2 70.513 78 16 1 33 103 322 399 1.86e-32 119
Msa0918700 AT1G04280.1 70.513 78 16 1 33 103 376 453 3.60e-32 119
Msa0918700 AT1G06750.2 55.556 117 35 5 14 123 349 455 3.19e-30 113
Msa0918700 AT1G06750.3 55.556 117 35 5 14 123 342 448 3.32e-30 113
Msa0918700 AT1G06750.1 55.556 117 35 5 14 123 342 448 3.32e-30 113
Msa0918700 AT2G30630.1 59.794 97 25 3 14 103 356 445 1.76e-29 111
Msa0918700 AT2G30630.2 59.794 97 25 3 14 103 377 466 1.76e-29 111

Find 22 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACAAGTTTGCAATATTT+TGG 0.119289 6_2:-47298241 None:intergenic
CTTGTAGCTCTGCTTCTGTT+AGG 0.288048 6_2:-47298932 None:intergenic
GGATATCTTGCAGTTATTAG+AGG 0.406489 6_2:+47298036 Msa0918700:CDS
TTGCAGTTATTAGAGGCATT+AGG 0.422529 6_2:+47298043 Msa0918700:CDS
TTGTAGCTCTGCTTCTGTTA+GGG 0.423721 6_2:-47298931 None:intergenic
CAACAAGAGGAAAATTCTAC+AGG 0.441415 6_2:+47297958 Msa0918700:CDS
GCAAACTTGTTGACAGTGCT+AGG 0.509508 6_2:+47298251 Msa0918700:CDS
CTAGTTGGTGTGGTTTGTGA+TGG 0.513280 6_2:+47298015 Msa0918700:CDS
CTATACAAAGCAAGTGAATG+AGG 0.513355 6_2:+47297921 Msa0918700:CDS
CCAACTAGCTCAATTCTGTA+AGG 0.525410 6_2:-47298000 None:intergenic
CCTTACAGAATTGAGCTAGT+TGG 0.526663 6_2:+47298000 Msa0918700:CDS
CAGAATTGAGCTAGTTGGTG+TGG 0.527716 6_2:+47298005 Msa0918700:CDS
CTCTATTGCACAGATGCTGT+TGG 0.532305 6_2:+47298274 Msa0918700:CDS
TATTGCACAGATGCTGTTGG+TGG 0.536088 6_2:+47298277 Msa0918700:CDS
TGCTGTTGGTGGTCCACCAA+AGG 0.575982 6_2:+47298288 Msa0918700:CDS
TATGACATGAAGGGCAGTGA+GGG 0.577678 6_2:+47298174 Msa0918700:intron
AGAAGAAGAACATCAACAAG+AGG 0.597156 6_2:+47297945 Msa0918700:CDS
AACAAGAGGAAAATTCTACA+GGG 0.606029 6_2:+47297959 Msa0918700:CDS
TATACAAAGCAAGTGAATGA+GGG 0.610525 6_2:+47297922 Msa0918700:CDS
AGTACTGCTTCAAGAAGTCG+AGG 0.646449 6_2:+47298953 Msa0918700:CDS
TTATGACATGAAGGGCAGTG+AGG 0.703176 6_2:+47298173 Msa0918700:intron
ACAAGAGGAAAATTCTACAG+GGG 0.709176 6_2:+47297960 Msa0918700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTACTATATTAATATACTT+TGG + chr6_2:47298084-47298103 Msa0918700:intron 15.0%
!!! TATAATTAACATTTTCCTTT+TGG + chr6_2:47298131-47298150 Msa0918700:intron 15.0%
!!! CTTTTCATTATTGATTTATT+GGG + chr6_2:47298550-47298569 Msa0918700:intron 15.0%
!!! TTTTCATTATTGATTTATTG+GGG + chr6_2:47298551-47298570 Msa0918700:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG - chr6_2:47298625-47298644 None:intergenic 15.0%
!! TCTACTATATTAATATACTT+TGG + chr6_2:47298084-47298103 Msa0918700:intron 15.0%
!!! TATAATTAACATTTTCCTTT+TGG + chr6_2:47298131-47298150 Msa0918700:intron 15.0%
!!! CTTTTCATTATTGATTTATT+GGG + chr6_2:47298550-47298569 Msa0918700:intron 15.0%
!!! TTTTCATTATTGATTTATTG+GGG + chr6_2:47298551-47298570 Msa0918700:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG - chr6_2:47298625-47298644 None:intergenic 15.0%
!! ACTATATTAATATACTTTGG+TGG + chr6_2:47298087-47298106 Msa0918700:intron 20.0%
!!! TTGTACATTTTTATTTCACA+TGG + chr6_2:47298465-47298484 Msa0918700:intron 20.0%
!! CCAATAAATCAATAATGAAA+AGG - chr6_2:47298552-47298571 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAT+TGG + chr6_2:47298549-47298568 Msa0918700:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG - chr6_2:47298621-47298640 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG - chr6_2:47298887-47298906 None:intergenic 20.0%
!! ACTATATTAATATACTTTGG+TGG + chr6_2:47298087-47298106 Msa0918700:intron 20.0%
!!! TTGTACATTTTTATTTCACA+TGG + chr6_2:47298465-47298484 Msa0918700:intron 20.0%
!! CCAATAAATCAATAATGAAA+AGG - chr6_2:47298552-47298571 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAT+TGG + chr6_2:47298549-47298568 Msa0918700:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG - chr6_2:47298621-47298640 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG - chr6_2:47298887-47298906 None:intergenic 20.0%
!!! ATACATGCATTTTTTGTGAA+AGG + chr6_2:47297895-47297914 Msa0918700:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG + chr6_2:47298143-47298162 Msa0918700:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG - chr6_2:47298244-47298263 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG - chr6_2:47298435-47298454 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG + chr6_2:47298790-47298809 Msa0918700:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG + chr6_2:47298818-47298837 Msa0918700:intron 25.0%
!!! ATACATGCATTTTTTGTGAA+AGG + chr6_2:47297895-47297914 Msa0918700:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG + chr6_2:47298143-47298162 Msa0918700:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG - chr6_2:47298244-47298263 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG - chr6_2:47298435-47298454 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG + chr6_2:47298790-47298809 Msa0918700:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG + chr6_2:47298818-47298837 Msa0918700:intron 25.0%
TATACAAAGCAAGTGAATGA+GGG + chr6_2:47297922-47297941 Msa0918700:CDS 30.0%
AACAAGAGGAAAATTCTACA+GGG + chr6_2:47297959-47297978 Msa0918700:CDS 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG + chr6_2:47298108-47298127 Msa0918700:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG - chr6_2:47298149-47298168 None:intergenic 30.0%
GAGAGTTATTATGACATGAA+GGG + chr6_2:47298165-47298184 Msa0918700:intron 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG + chr6_2:47298394-47298413 Msa0918700:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG - chr6_2:47298503-47298522 None:intergenic 30.0%
CTTATTAGCTCAGATATCAA+AGG - chr6_2:47298650-47298669 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG + chr6_2:47298817-47298836 Msa0918700:intron 30.0%
TATACAAAGCAAGTGAATGA+GGG + chr6_2:47297922-47297941 Msa0918700:CDS 30.0%
AACAAGAGGAAAATTCTACA+GGG + chr6_2:47297959-47297978 Msa0918700:CDS 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG + chr6_2:47298108-47298127 Msa0918700:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG - chr6_2:47298149-47298168 None:intergenic 30.0%
GAGAGTTATTATGACATGAA+GGG + chr6_2:47298165-47298184 Msa0918700:intron 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG + chr6_2:47298394-47298413 Msa0918700:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG - chr6_2:47298503-47298522 None:intergenic 30.0%
CTTATTAGCTCAGATATCAA+AGG - chr6_2:47298650-47298669 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG + chr6_2:47298817-47298836 Msa0918700:intron 30.0%
CTATACAAAGCAAGTGAATG+AGG + chr6_2:47297921-47297940 Msa0918700:CDS 35.0%
AGAAGAAGAACATCAACAAG+AGG + chr6_2:47297945-47297964 Msa0918700:CDS 35.0%
CAACAAGAGGAAAATTCTAC+AGG + chr6_2:47297958-47297977 Msa0918700:CDS 35.0%
ACAAGAGGAAAATTCTACAG+GGG + chr6_2:47297960-47297979 Msa0918700:CDS 35.0%
GGATATCTTGCAGTTATTAG+AGG + chr6_2:47298036-47298055 Msa0918700:CDS 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG + chr6_2:47298043-47298062 Msa0918700:CDS 35.0%
GGAGAGTTATTATGACATGA+AGG + chr6_2:47298164-47298183 Msa0918700:intron 35.0%
!!! GTTGCTCTTGTTTTTACCTT+TGG - chr6_2:47298307-47298326 None:intergenic 35.0%
! TCTTTGGCCAAAACGATTTT+AGG - chr6_2:47298404-47298423 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG + chr6_2:47298571-47298590 Msa0918700:intron 35.0%
GAATGTTTAACAGTCTCTCA+GGG - chr6_2:47298742-47298761 None:intergenic 35.0%
TGAATGTTTAACAGTCTCTC+AGG - chr6_2:47298743-47298762 None:intergenic 35.0%
ACTTCAGTCATCTATACCTT+CGG - chr6_2:47298809-47298828 None:intergenic 35.0%
CTATACAAAGCAAGTGAATG+AGG + chr6_2:47297921-47297940 Msa0918700:CDS 35.0%
AGAAGAAGAACATCAACAAG+AGG + chr6_2:47297945-47297964 Msa0918700:CDS 35.0%
CAACAAGAGGAAAATTCTAC+AGG + chr6_2:47297958-47297977 Msa0918700:CDS 35.0%
ACAAGAGGAAAATTCTACAG+GGG + chr6_2:47297960-47297979 Msa0918700:CDS 35.0%
GGATATCTTGCAGTTATTAG+AGG + chr6_2:47298036-47298055 Msa0918700:CDS 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG + chr6_2:47298043-47298062 Msa0918700:CDS 35.0%
GGAGAGTTATTATGACATGA+AGG + chr6_2:47298164-47298183 Msa0918700:intron 35.0%
!!! GTTGCTCTTGTTTTTACCTT+TGG - chr6_2:47298307-47298326 None:intergenic 35.0%
! TCTTTGGCCAAAACGATTTT+AGG - chr6_2:47298404-47298423 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG + chr6_2:47298571-47298590 Msa0918700:intron 35.0%
GAATGTTTAACAGTCTCTCA+GGG - chr6_2:47298742-47298761 None:intergenic 35.0%
TGAATGTTTAACAGTCTCTC+AGG - chr6_2:47298743-47298762 None:intergenic 35.0%
ACTTCAGTCATCTATACCTT+CGG - chr6_2:47298809-47298828 None:intergenic 35.0%
CCAACTAGCTCAATTCTGTA+AGG - chr6_2:47298003-47298022 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG + chr6_2:47298000-47298019 Msa0918700:CDS 40.0%
!!! GCTCTTGTTTTTACCTTTGG+TGG - chr6_2:47298304-47298323 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG + chr6_2:47298409-47298428 Msa0918700:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG - chr6_2:47298420-47298439 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG + chr6_2:47298901-47298920 Msa0918700:intron 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG - chr6_2:47298934-47298953 None:intergenic 40.0%
CCAACTAGCTCAATTCTGTA+AGG - chr6_2:47298003-47298022 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG + chr6_2:47298000-47298019 Msa0918700:CDS 40.0%
!!! GCTCTTGTTTTTACCTTTGG+TGG - chr6_2:47298304-47298323 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG + chr6_2:47298409-47298428 Msa0918700:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG - chr6_2:47298420-47298439 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG + chr6_2:47298901-47298920 Msa0918700:intron 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG - chr6_2:47298934-47298953 None:intergenic 40.0%
! CAGAATTGAGCTAGTTGGTG+TGG + chr6_2:47298005-47298024 Msa0918700:CDS 45.0%
! CTAGTTGGTGTGGTTTGTGA+TGG + chr6_2:47298015-47298034 Msa0918700:CDS 45.0%
TTATGACATGAAGGGCAGTG+AGG + chr6_2:47298173-47298192 Msa0918700:intron 45.0%
TATGACATGAAGGGCAGTGA+GGG + chr6_2:47298174-47298193 Msa0918700:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG + chr6_2:47298251-47298270 Msa0918700:CDS 45.0%
CTCTATTGCACAGATGCTGT+TGG + chr6_2:47298274-47298293 Msa0918700:CDS 45.0%
TATTGCACAGATGCTGTTGG+TGG + chr6_2:47298277-47298296 Msa0918700:CDS 45.0%
!! AGTCTCTCAGGGTTGATACA+TGG - chr6_2:47298731-47298750 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG + chr6_2:47298829-47298848 Msa0918700:intron 45.0%
CGCTTCTGCAGATTCAATAG+AGG - chr6_2:47298903-47298922 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG - chr6_2:47298935-47298954 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG + chr6_2:47298953-47298972 Msa0918700:CDS 45.0%
! CAGAATTGAGCTAGTTGGTG+TGG + chr6_2:47298005-47298024 Msa0918700:CDS 45.0%
! CTAGTTGGTGTGGTTTGTGA+TGG + chr6_2:47298015-47298034 Msa0918700:CDS 45.0%
TTATGACATGAAGGGCAGTG+AGG + chr6_2:47298173-47298192 Msa0918700:intron 45.0%
TATGACATGAAGGGCAGTGA+GGG + chr6_2:47298174-47298193 Msa0918700:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG + chr6_2:47298251-47298270 Msa0918700:CDS 45.0%
CTCTATTGCACAGATGCTGT+TGG + chr6_2:47298274-47298293 Msa0918700:CDS 45.0%
TATTGCACAGATGCTGTTGG+TGG + chr6_2:47298277-47298296 Msa0918700:CDS 45.0%
!! AGTCTCTCAGGGTTGATACA+TGG - chr6_2:47298731-47298750 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG + chr6_2:47298829-47298848 Msa0918700:intron 45.0%
CGCTTCTGCAGATTCAATAG+AGG - chr6_2:47298903-47298922 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG - chr6_2:47298935-47298954 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG + chr6_2:47298953-47298972 Msa0918700:CDS 45.0%
!! TGCTGTTGGTGGTCCACCAA+AGG + chr6_2:47298288-47298307 Msa0918700:CDS 55.0%
!! TGCTGTTGGTGGTCCACCAA+AGG + chr6_2:47298288-47298307 Msa0918700:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 47297880 47298992 47297880 ID=Msa0918700;Name=Msa0918700
chr6_2 mRNA 47297880 47298992 47297880 ID=Msa0918700-mRNA-1;Parent=Msa0918700;Name=Msa0918700-mRNA-1;_AED=0.19;_eAED=0.53;_QI=0|0|0|0.66|0.5|0.66|3|0|127
chr6_2 exon 47297880 47298064 47297880 ID=Msa0918700-mRNA-1:exon:7320;Parent=Msa0918700-mRNA-1
chr6_2 exon 47298186 47298309 47298186 ID=Msa0918700-mRNA-1:exon:7321;Parent=Msa0918700-mRNA-1
chr6_2 exon 47298918 47298992 47298918 ID=Msa0918700-mRNA-1:exon:7322;Parent=Msa0918700-mRNA-1
chr6_2 CDS 47297880 47298064 47297880 ID=Msa0918700-mRNA-1:cds;Parent=Msa0918700-mRNA-1
chr6_2 CDS 47298186 47298309 47298186 ID=Msa0918700-mRNA-1:cds;Parent=Msa0918700-mRNA-1
chr6_2 CDS 47298918 47298992 47298918 ID=Msa0918700-mRNA-1:cds;Parent=Msa0918700-mRNA-1
Gene Sequence

>Msa0918700

ATGATTATTCAAACTATACATGCATTTTTTGTGAAAGGAAACTATACAAAGCAAGTGAATGAGGGAGAAGAAGAACATCAACAAGAGGAAAATTCTACAGGGGAACTACACTCTCAAAAGCCTTACAGAATTGAGCTAGTTGGTGTGGTTTGTGATGGATATCTTGCAGTTATTAGAGGCATTAGGGCAGTGAGGGTGAATTCACAATTGCAATCTCACAAAAGATTTTCTAATGCATTTCCAAAATATTGCAAACTTGTTGACAGTGCTAGGCTCTATTGCACAGATGCTGTTGGTGGTCCACCAAAGAAGCGGTTAGAGTCCCTAACAGAAGCAGAGCTACAAGTACTGCTTCAAGAAGTCGAGGAAGAGAAGAGTCGTTAG

Protein sequence

>Msa0918700

MIIQTIHAFFVKGNYTKQVNEGEEEHQQEENSTGELHSQKPYRIELVGVVCDGYLAVIRGIRAVRVNSQLQSHKRFSNAFPKYCKLVDSARLYCTDAVGGPPKKRLESLTEAELQVLLQEVEEEKSR*