AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1273920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1273920 MtrunA17_Chr1g0187021 88.660 97 3 2 14 103 344 439 6.48e-54 176
Msa1273920 MtrunA17_Chr1g0187071 71.134 97 20 2 14 103 343 438 1.38e-38 135
Msa1273920 MtrunA17_Chr1g0187051 68.041 97 13 2 14 103 339 424 2.37e-37 132
Msa1273920 MtrunA17_Chr7g0260781 69.444 72 15 1 39 103 223 294 6.21e-28 105
Msa1273920 MtrunA17_Chr1g0184551 61.628 86 24 2 25 103 347 430 2.58e-27 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1273920 AT1G04280.2 70.513 78 16 1 33 103 322 399 1.16e-31 117
Msa1273920 AT1G04280.1 70.513 78 16 1 33 103 376 453 2.01e-31 116
Msa1273920 AT1G06750.2 55.556 117 35 5 14 123 349 455 1.08e-29 112
Msa1273920 AT1G06750.3 55.556 117 35 5 14 123 342 448 1.22e-29 111
Msa1273920 AT1G06750.1 55.556 117 35 5 14 123 342 448 1.22e-29 111
Msa1273920 AT2G30630.1 59.794 97 25 3 14 103 356 445 7.92e-29 109
Msa1273920 AT2G30630.2 59.794 97 25 3 14 103 377 466 9.07e-29 109

Find 22 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACAAGTTTGCAATATTT+TGG 0.119289 8_3:+7665355 None:intergenic
CTTGTAGCTCTGCTTCTGTT+AGG 0.288048 8_3:+7664652 None:intergenic
GGATATCTTGCAGTTATTAG+AGG 0.406489 8_3:-7665560 Msa1273920:CDS
TTGCAGTTATTAGAGGCATT+AGG 0.422529 8_3:-7665553 Msa1273920:intron
TTGTAGCTCTGCTTCTGTTA+GGG 0.423721 8_3:+7664653 None:intergenic
CAACAAGAGGAAAATTCTAC+AGG 0.441415 8_3:-7665638 Msa1273920:CDS
GCAAACTTGTTGACAGTGCT+AGG 0.509508 8_3:-7665345 Msa1273920:CDS
CTAGTTGGTGTGGTTTGTGA+TGG 0.513280 8_3:-7665581 Msa1273920:CDS
CTATACAAAGCAAGTGAATG+AGG 0.513355 8_3:-7665675 Msa1273920:CDS
CCAACTAGCTCAATTCTGTA+AGG 0.525410 8_3:+7665596 None:intergenic
CCTTACAGAATTGAGCTAGT+TGG 0.526663 8_3:-7665596 Msa1273920:CDS
CAGAATTGAGCTAGTTGGTG+TGG 0.527716 8_3:-7665591 Msa1273920:CDS
CTCTATTGCACAGATGCTGT+TGG 0.532305 8_3:-7665322 Msa1273920:CDS
TATTGCACAGATGCTGTTGG+TGG 0.536088 8_3:-7665319 Msa1273920:CDS
TGCTGTTGGTGGTCCACCAA+AGG 0.575982 8_3:-7665308 Msa1273920:intron
TATGACATGAAGGGCAGTGA+GGG 0.577678 8_3:-7665422 Msa1273920:intron
AGAAGAAGAACATCAACAAG+AGG 0.597156 8_3:-7665651 Msa1273920:CDS
AACAAGAGGAAAATTCTACA+GGG 0.606029 8_3:-7665637 Msa1273920:CDS
TATACAAAGCAAGTGAATGA+GGG 0.610525 8_3:-7665674 Msa1273920:CDS
AGTACTGCTTCAAGAAGTCG+AGG 0.646449 8_3:-7664631 Msa1273920:CDS
TTATGACATGAAGGGCAGTG+AGG 0.692934 8_3:-7665423 Msa1273920:intron
ACAAGAGGAAAATTCTACAG+GGG 0.709176 8_3:-7665636 Msa1273920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAGTATATTAATATAGTA+GGG + chr8_3:7664820-7664839 None:intergenic 15.0%
!!! TATAATTAACATTTTCCTTT+TGG - chr8_3:7664865-7664884 Msa1273920:intron 15.0%
!!! CTTTTCATTATTGATTTATT+GGG - chr8_3:7665302-7665321 Msa1273920:intron 15.0%
!!! TTTTCATTATTGATTTATTG+GGG - chr8_3:7665303-7665322 Msa1273920:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG + chr8_3:7665377-7665396 None:intergenic 15.0%
!! CAAAGTATATTAATATAGTA+GGG + chr8_3:7664820-7664839 None:intergenic 15.0%
!!! TATAATTAACATTTTCCTTT+TGG - chr8_3:7664865-7664884 Msa1273920:intron 15.0%
!!! CTTTTCATTATTGATTTATT+GGG - chr8_3:7665302-7665321 Msa1273920:intron 15.0%
!!! TTTTCATTATTGATTTATTG+GGG - chr8_3:7665303-7665322 Msa1273920:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG + chr8_3:7665377-7665396 None:intergenic 15.0%
!! CCAAAGTATATTAATATAGT+AGG + chr8_3:7664821-7664840 None:intergenic 20.0%
!! CCTACTATATTAATATACTT+TGG - chr8_3:7664818-7664837 Msa1273920:intron 20.0%
!! ACTATATTAATATACTTTGG+TGG - chr8_3:7664821-7664840 Msa1273920:intron 20.0%
!!! TTGTACTTTTTTATTTCACA+TGG - chr8_3:7665199-7665218 Msa1273920:intron 20.0%
!! CCAATAAATCAATAATGAAA+AGG + chr8_3:7665304-7665323 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAT+TGG - chr8_3:7665301-7665320 Msa1273920:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG + chr8_3:7665373-7665392 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG + chr8_3:7665633-7665652 None:intergenic 20.0%
!! CCAAAGTATATTAATATAGT+AGG + chr8_3:7664821-7664840 None:intergenic 20.0%
!! CCTACTATATTAATATACTT+TGG - chr8_3:7664818-7664837 Msa1273920:intron 20.0%
!! ACTATATTAATATACTTTGG+TGG - chr8_3:7664821-7664840 Msa1273920:intron 20.0%
!!! TTGTACTTTTTTATTTCACA+TGG - chr8_3:7665199-7665218 Msa1273920:intron 20.0%
!! CCAATAAATCAATAATGAAA+AGG + chr8_3:7665304-7665323 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAT+TGG - chr8_3:7665301-7665320 Msa1273920:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG + chr8_3:7665373-7665392 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG + chr8_3:7665633-7665652 None:intergenic 20.0%
!!! ATACATGCATTTTTTGTGAA+AGG - chr8_3:7664629-7664648 Msa1273920:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG - chr8_3:7664877-7664896 Msa1273920:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG + chr8_3:7664978-7664997 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG + chr8_3:7665169-7665188 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG - chr8_3:7665536-7665555 Msa1273920:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG - chr8_3:7665564-7665583 Msa1273920:CDS 25.0%
!!! ATACATGCATTTTTTGTGAA+AGG - chr8_3:7664629-7664648 Msa1273920:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG - chr8_3:7664877-7664896 Msa1273920:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG + chr8_3:7664978-7664997 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG + chr8_3:7665169-7665188 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG - chr8_3:7665536-7665555 Msa1273920:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG - chr8_3:7665564-7665583 Msa1273920:CDS 25.0%
TATACAAAGCAAGTGAATGA+GGG - chr8_3:7664656-7664675 Msa1273920:CDS 30.0%
AACAAGAGGAAAATTCTACA+GGG - chr8_3:7664693-7664712 Msa1273920:intron 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG - chr8_3:7664842-7664861 Msa1273920:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG + chr8_3:7664883-7664902 None:intergenic 30.0%
GAGAACTATTATGACATGAA+GGG - chr8_3:7664899-7664918 Msa1273920:intron 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG - chr8_3:7665128-7665147 Msa1273920:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG + chr8_3:7665237-7665256 None:intergenic 30.0%
CTTATTAGCTCAGATATCAA+AGG + chr8_3:7665402-7665421 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG - chr8_3:7665563-7665582 Msa1273920:CDS 30.0%
TATACAAAGCAAGTGAATGA+GGG - chr8_3:7664656-7664675 Msa1273920:CDS 30.0%
AACAAGAGGAAAATTCTACA+GGG - chr8_3:7664693-7664712 Msa1273920:intron 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG - chr8_3:7664842-7664861 Msa1273920:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG + chr8_3:7664883-7664902 None:intergenic 30.0%
GAGAACTATTATGACATGAA+GGG - chr8_3:7664899-7664918 Msa1273920:intron 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG - chr8_3:7665128-7665147 Msa1273920:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG + chr8_3:7665237-7665256 None:intergenic 30.0%
CTTATTAGCTCAGATATCAA+AGG + chr8_3:7665402-7665421 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG - chr8_3:7665563-7665582 Msa1273920:CDS 30.0%
CTATACAAAGCAAGTGAATG+AGG - chr8_3:7664655-7664674 Msa1273920:CDS 35.0%
AGAAGAAGAACATCAACAAG+AGG - chr8_3:7664679-7664698 Msa1273920:intron 35.0%
CAACAAGAGGAAAATTCTAC+AGG - chr8_3:7664692-7664711 Msa1273920:intron 35.0%
ACAAGAGGAAAATTCTACAG+GGG - chr8_3:7664694-7664713 Msa1273920:intron 35.0%
GGATATCTTGCAGTTATTAG+AGG - chr8_3:7664770-7664789 Msa1273920:intron 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG - chr8_3:7664777-7664796 Msa1273920:intron 35.0%
GGAGAACTATTATGACATGA+AGG - chr8_3:7664898-7664917 Msa1273920:intron 35.0%
! TCTTTGGCCAAAACGATTTT+AGG + chr8_3:7665138-7665157 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG - chr8_3:7665323-7665342 Msa1273920:CDS 35.0%
GAATGTTTAACAGTCTCTCA+GGG + chr8_3:7665494-7665513 None:intergenic 35.0%
TGAATGTTTAACAGTCTCTC+AGG + chr8_3:7665495-7665514 None:intergenic 35.0%
ACTTCAGTCATCTATACCTT+CGG + chr8_3:7665555-7665574 None:intergenic 35.0%
CTATACAAAGCAAGTGAATG+AGG - chr8_3:7664655-7664674 Msa1273920:CDS 35.0%
AGAAGAAGAACATCAACAAG+AGG - chr8_3:7664679-7664698 Msa1273920:intron 35.0%
CAACAAGAGGAAAATTCTAC+AGG - chr8_3:7664692-7664711 Msa1273920:intron 35.0%
ACAAGAGGAAAATTCTACAG+GGG - chr8_3:7664694-7664713 Msa1273920:intron 35.0%
GGATATCTTGCAGTTATTAG+AGG - chr8_3:7664770-7664789 Msa1273920:intron 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG - chr8_3:7664777-7664796 Msa1273920:intron 35.0%
GGAGAACTATTATGACATGA+AGG - chr8_3:7664898-7664917 Msa1273920:intron 35.0%
! TCTTTGGCCAAAACGATTTT+AGG + chr8_3:7665138-7665157 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG - chr8_3:7665323-7665342 Msa1273920:CDS 35.0%
GAATGTTTAACAGTCTCTCA+GGG + chr8_3:7665494-7665513 None:intergenic 35.0%
TGAATGTTTAACAGTCTCTC+AGG + chr8_3:7665495-7665514 None:intergenic 35.0%
ACTTCAGTCATCTATACCTT+CGG + chr8_3:7665555-7665574 None:intergenic 35.0%
CCAACTAGCTCAATTCTGTA+AGG + chr8_3:7664737-7664756 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG - chr8_3:7664734-7664753 Msa1273920:intron 40.0%
!!! GTTGCTCCTGTTTTTACCTT+TGG + chr8_3:7665041-7665060 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG - chr8_3:7665143-7665162 Msa1273920:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG + chr8_3:7665154-7665173 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG - chr8_3:7665647-7665666 Msa1273920:CDS 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG + chr8_3:7665680-7665699 None:intergenic 40.0%
CCAACTAGCTCAATTCTGTA+AGG + chr8_3:7664737-7664756 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG - chr8_3:7664734-7664753 Msa1273920:intron 40.0%
!!! GTTGCTCCTGTTTTTACCTT+TGG + chr8_3:7665041-7665060 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG - chr8_3:7665143-7665162 Msa1273920:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG + chr8_3:7665154-7665173 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG - chr8_3:7665647-7665666 Msa1273920:CDS 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG + chr8_3:7665680-7665699 None:intergenic 40.0%
! CAGAATTGAGCTAGTTGGTG+TGG - chr8_3:7664739-7664758 Msa1273920:intron 45.0%
! CTAGTTGGTGTGGTTTGTGA+TGG - chr8_3:7664749-7664768 Msa1273920:intron 45.0%
TTATGACATGAAGGGCAGTG+AGG - chr8_3:7664907-7664926 Msa1273920:intron 45.0%
TATGACATGAAGGGCAGTGA+GGG - chr8_3:7664908-7664927 Msa1273920:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG - chr8_3:7664985-7665004 Msa1273920:intron 45.0%
CTCTATTGCACAGATGCTGT+TGG - chr8_3:7665008-7665027 Msa1273920:intron 45.0%
TATTGCACAGATGCTGTTGG+TGG - chr8_3:7665011-7665030 Msa1273920:intron 45.0%
GGTCCACCAAAGGTAAAAAC+AGG - chr8_3:7665032-7665051 Msa1273920:intron 45.0%
!!! GCTCCTGTTTTTACCTTTGG+TGG + chr8_3:7665038-7665057 None:intergenic 45.0%
!! AGTCTCTCAGGGTTGATACA+TGG + chr8_3:7665483-7665502 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG - chr8_3:7665575-7665594 Msa1273920:CDS 45.0%
CGCTTCTGCAGATTCAATAG+AGG + chr8_3:7665649-7665668 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG + chr8_3:7665681-7665700 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG - chr8_3:7665699-7665718 Msa1273920:CDS 45.0%
! CAGAATTGAGCTAGTTGGTG+TGG - chr8_3:7664739-7664758 Msa1273920:intron 45.0%
! CTAGTTGGTGTGGTTTGTGA+TGG - chr8_3:7664749-7664768 Msa1273920:intron 45.0%
TTATGACATGAAGGGCAGTG+AGG - chr8_3:7664907-7664926 Msa1273920:intron 45.0%
TATGACATGAAGGGCAGTGA+GGG - chr8_3:7664908-7664927 Msa1273920:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG - chr8_3:7664985-7665004 Msa1273920:intron 45.0%
CTCTATTGCACAGATGCTGT+TGG - chr8_3:7665008-7665027 Msa1273920:intron 45.0%
TATTGCACAGATGCTGTTGG+TGG - chr8_3:7665011-7665030 Msa1273920:intron 45.0%
GGTCCACCAAAGGTAAAAAC+AGG - chr8_3:7665032-7665051 Msa1273920:intron 45.0%
!!! GCTCCTGTTTTTACCTTTGG+TGG + chr8_3:7665038-7665057 None:intergenic 45.0%
!! AGTCTCTCAGGGTTGATACA+TGG + chr8_3:7665483-7665502 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG - chr8_3:7665575-7665594 Msa1273920:CDS 45.0%
CGCTTCTGCAGATTCAATAG+AGG + chr8_3:7665649-7665668 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG + chr8_3:7665681-7665700 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG - chr8_3:7665699-7665718 Msa1273920:CDS 45.0%
!! TGCTGTTGGTGGTCCACCAA+AGG - chr8_3:7665022-7665041 Msa1273920:intron 55.0%
!! TGCTGTTGGTGGTCCACCAA+AGG - chr8_3:7665022-7665041 Msa1273920:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 7664614 7665738 7664614 ID=Msa1273920;Name=Msa1273920
chr8_3 mRNA 7664614 7665738 7664614 ID=Msa1273920-mRNA-1;Parent=Msa1273920;Name=Msa1273920-mRNA-1;_AED=0.19;_eAED=0.53;_QI=0|0|0|0.66|1|1|3|0|127
chr8_3 exon 7665554 7665738 7665554 ID=Msa1273920-mRNA-1:exon:3039;Parent=Msa1273920-mRNA-1
chr8_3 exon 7665309 7665432 7665309 ID=Msa1273920-mRNA-1:exon:3038;Parent=Msa1273920-mRNA-1
chr8_3 exon 7664614 7664688 7664614 ID=Msa1273920-mRNA-1:exon:3037;Parent=Msa1273920-mRNA-1
chr8_3 CDS 7665554 7665738 7665554 ID=Msa1273920-mRNA-1:cds;Parent=Msa1273920-mRNA-1
chr8_3 CDS 7665309 7665432 7665309 ID=Msa1273920-mRNA-1:cds;Parent=Msa1273920-mRNA-1
chr8_3 CDS 7664614 7664688 7664614 ID=Msa1273920-mRNA-1:cds;Parent=Msa1273920-mRNA-1
Gene Sequence

>Msa1273920

ATGATTATTCAAACTATACATGCATTTTTTGTGAAAGGAAACTATACAAAGCAAGTGAATGAGGGAGAAGAAGAACATCAACAAGAGGAAAATTCTACAGGGGAACTACACTCTCAAAAGCCTTACAGAATTGAGCTAGTTGGTGTGGTTTGTGATGGATATCTTGCAGTTATTAGAGGCATTAGGGCAGTGAGGGTGAATTCACAATTGCAATCTAACAAAAGATTTGCTAATGCATTTCCAAAATATTGCAAACTTGTTGACAGTGCTAGGCTCTATTGCACAGATGCTGTTGGTGGTCCACCAAAGAAGCGGTTAGAGTCCCTAACAGAAGCAGAGCTACAAGTACTGCTTCAAGAAGTCGAGGAAGAGAAGAGTCGTTAG

Protein sequence

>Msa1273920

MIIQTIHAFFVKGNYTKQVNEGEEEHQQEENSTGELHSQKPYRIELVGVVCDGYLAVIRGIRAVRVNSQLQSNKRFANAFPKYCKLVDSARLYCTDAVGGPPKKRLESLTEAELQVLLQEVEEEKSR*