AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0401310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0401310 MtrunA17_Chr1g0187021 88.660 97 3 2 14 103 344 439 2.23e-54 177
Msa0401310 MtrunA17_Chr1g0187071 72.165 97 19 2 14 103 343 438 1.57e-39 138
Msa0401310 MtrunA17_Chr1g0187051 69.072 97 12 2 14 103 339 424 2.18e-38 135
Msa0401310 MtrunA17_Chr7g0260781 57.000 100 29 3 11 103 202 294 4.93e-29 108
Msa0401310 MtrunA17_Chr1g0184551 63.953 86 22 2 25 103 347 430 8.66e-29 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0401310 AT1G04280.2 71.795 78 15 1 33 103 322 399 7.58e-33 120
Msa0401310 AT1G04280.1 71.795 78 15 1 33 103 376 453 1.33e-32 120
Msa0401310 AT1G06750.2 55.556 117 35 5 14 123 349 455 4.13e-30 113
Msa0401310 AT1G06750.3 55.556 117 35 5 14 123 342 448 4.14e-30 113
Msa0401310 AT1G06750.1 55.556 117 35 5 14 123 342 448 4.14e-30 113
Msa0401310 AT2G30630.1 59.794 97 25 3 14 103 356 445 2.23e-29 111
Msa0401310 AT2G30630.2 59.794 97 25 3 14 103 377 466 2.55e-29 110

Find 22 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACAAGTTTGCAATATTT+TGG 0.119289 3_2:-26824970 None:intergenic
CTTGTAGCTCTGCTTCTGTT+AGG 0.288048 3_2:-26825672 None:intergenic
GGATATCTTGCAGTTATTAG+AGG 0.406489 3_2:+26824765 Msa0401310:CDS
TTGCAGTTATTAGAGGCATT+AGG 0.422529 3_2:+26824772 Msa0401310:CDS
TTGTAGCTCTGCTTCTGTTA+GGG 0.423721 3_2:-26825671 None:intergenic
CAAGAAGAGGAAAATTCTAC+AGG 0.430874 3_2:+26824687 Msa0401310:CDS
GCAAACTTGTTGACAGTGCT+AGG 0.509508 3_2:+26824980 Msa0401310:CDS
CTAGTTGGTGTGGTTTGTGA+TGG 0.513280 3_2:+26824744 Msa0401310:CDS
CTATACAAAGCAAGTGAATG+AGG 0.513355 3_2:+26824650 Msa0401310:CDS
CCAACTAGCTCAATTCTGTA+AGG 0.525410 3_2:-26824729 None:intergenic
CCTTACAGAATTGAGCTAGT+TGG 0.526663 3_2:+26824729 Msa0401310:CDS
CAGAATTGAGCTAGTTGGTG+TGG 0.527716 3_2:+26824734 Msa0401310:CDS
CTCTATTGCACAGATGCTGT+TGG 0.532305 3_2:+26825003 Msa0401310:CDS
TATTGCACAGATGCTGTTGG+TGG 0.536088 3_2:+26825006 Msa0401310:CDS
TGCTGTTGGTGGTCCACCAA+AGG 0.575982 3_2:+26825017 Msa0401310:CDS
AGAAGAAGAACATCAAGAAG+AGG 0.576728 3_2:+26824674 Msa0401310:CDS
TATGACATGAAGGGCAGTGA+GGG 0.577678 3_2:+26824903 Msa0401310:intron
TATACAAAGCAAGTGAATGA+GGG 0.610525 3_2:+26824651 Msa0401310:CDS
AAGAAGAGGAAAATTCTACA+GGG 0.618161 3_2:+26824688 Msa0401310:CDS
AGTACTGCTTCAAGAAGTCG+AGG 0.646449 3_2:+26825693 Msa0401310:CDS
AGAAGAGGAAAATTCTACAG+GGG 0.696758 3_2:+26824689 Msa0401310:CDS
TTATGACATGAAGGGCAGTG+AGG 0.703176 3_2:+26824902 Msa0401310:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTAATATATTAATATACTT+TGG + chr3_2:26824813-26824832 Msa0401310:intron 10.0%
!! TCTAATATATTAATATACTT+TGG + chr3_2:26824813-26824832 Msa0401310:intron 10.0%
!! AATATATTAATATACTTTGG+TGG + chr3_2:26824816-26824835 Msa0401310:intron 15.0%
!!! TATAATTAACATTTTCCTTT+TGG + chr3_2:26824860-26824879 Msa0401310:intron 15.0%
!!! CTTTTCATTATTGATTTAAT+GGG + chr3_2:26825297-26825316 Msa0401310:intron 15.0%
!!! TTTTCATTATTGATTTAATG+GGG + chr3_2:26825298-26825317 Msa0401310:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG - chr3_2:26825372-26825391 None:intergenic 15.0%
!! AATATATTAATATACTTTGG+TGG + chr3_2:26824816-26824835 Msa0401310:intron 15.0%
!!! TATAATTAACATTTTCCTTT+TGG + chr3_2:26824860-26824879 Msa0401310:intron 15.0%
!!! CTTTTCATTATTGATTTAAT+GGG + chr3_2:26825297-26825316 Msa0401310:intron 15.0%
!!! TTTTCATTATTGATTTAATG+GGG + chr3_2:26825298-26825317 Msa0401310:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG - chr3_2:26825372-26825391 None:intergenic 15.0%
!!! TTGTACATTTTTATTTCACA+TGG + chr3_2:26825194-26825213 Msa0401310:intron 20.0%
!! CCATTAAATCAATAATGAAA+AGG - chr3_2:26825299-26825318 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAA+TGG + chr3_2:26825296-26825315 Msa0401310:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG - chr3_2:26825368-26825387 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG - chr3_2:26825627-26825646 None:intergenic 20.0%
!!! TTGTACATTTTTATTTCACA+TGG + chr3_2:26825194-26825213 Msa0401310:intron 20.0%
!! CCATTAAATCAATAATGAAA+AGG - chr3_2:26825299-26825318 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAA+TGG + chr3_2:26825296-26825315 Msa0401310:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG - chr3_2:26825368-26825387 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG - chr3_2:26825627-26825646 None:intergenic 20.0%
!!! ATACATGCATTTTTTGTGAA+AGG + chr3_2:26824624-26824643 Msa0401310:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG + chr3_2:26824872-26824891 Msa0401310:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG - chr3_2:26824973-26824992 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG - chr3_2:26825164-26825183 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG + chr3_2:26825530-26825549 Msa0401310:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG + chr3_2:26825558-26825577 Msa0401310:intron 25.0%
!!! ATACATGCATTTTTTGTGAA+AGG + chr3_2:26824624-26824643 Msa0401310:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG + chr3_2:26824872-26824891 Msa0401310:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG - chr3_2:26824973-26824992 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG - chr3_2:26825164-26825183 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG + chr3_2:26825530-26825549 Msa0401310:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG + chr3_2:26825558-26825577 Msa0401310:intron 25.0%
TATACAAAGCAAGTGAATGA+GGG + chr3_2:26824651-26824670 Msa0401310:CDS 30.0%
AAGAAGAGGAAAATTCTACA+GGG + chr3_2:26824688-26824707 Msa0401310:CDS 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG + chr3_2:26824837-26824856 Msa0401310:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG - chr3_2:26824878-26824897 None:intergenic 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG + chr3_2:26825123-26825142 Msa0401310:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG - chr3_2:26825232-26825251 None:intergenic 30.0%
CTTATTAGCTCAGATATCAA+AGG - chr3_2:26825397-26825416 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG + chr3_2:26825557-26825576 Msa0401310:intron 30.0%
TATACAAAGCAAGTGAATGA+GGG + chr3_2:26824651-26824670 Msa0401310:CDS 30.0%
AAGAAGAGGAAAATTCTACA+GGG + chr3_2:26824688-26824707 Msa0401310:CDS 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG + chr3_2:26824837-26824856 Msa0401310:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG - chr3_2:26824878-26824897 None:intergenic 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG + chr3_2:26825123-26825142 Msa0401310:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG - chr3_2:26825232-26825251 None:intergenic 30.0%
CTTATTAGCTCAGATATCAA+AGG - chr3_2:26825397-26825416 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG + chr3_2:26825557-26825576 Msa0401310:intron 30.0%
CTATACAAAGCAAGTGAATG+AGG + chr3_2:26824650-26824669 Msa0401310:CDS 35.0%
AGAAGAAGAACATCAAGAAG+AGG + chr3_2:26824674-26824693 Msa0401310:CDS 35.0%
CAAGAAGAGGAAAATTCTAC+AGG + chr3_2:26824687-26824706 Msa0401310:CDS 35.0%
AGAAGAGGAAAATTCTACAG+GGG + chr3_2:26824689-26824708 Msa0401310:CDS 35.0%
GGATATCTTGCAGTTATTAG+AGG + chr3_2:26824765-26824784 Msa0401310:CDS 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG + chr3_2:26824772-26824791 Msa0401310:CDS 35.0%
GAGAGCTATTATGACATGAA+GGG + chr3_2:26824894-26824913 Msa0401310:intron 35.0%
! TCTTTGGCCAAAACGATTTT+AGG - chr3_2:26825133-26825152 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG + chr3_2:26825318-26825337 Msa0401310:intron 35.0%
GAATGTTTAACAGTCTCTCA+GGG - chr3_2:26825489-26825508 None:intergenic 35.0%
TGAATGTTTAACAGTCTCTC+AGG - chr3_2:26825490-26825509 None:intergenic 35.0%
ACTTCAGTCATCTATACCTT+CGG - chr3_2:26825549-26825568 None:intergenic 35.0%
CTATACAAAGCAAGTGAATG+AGG + chr3_2:26824650-26824669 Msa0401310:CDS 35.0%
AGAAGAAGAACATCAAGAAG+AGG + chr3_2:26824674-26824693 Msa0401310:CDS 35.0%
CAAGAAGAGGAAAATTCTAC+AGG + chr3_2:26824687-26824706 Msa0401310:CDS 35.0%
AGAAGAGGAAAATTCTACAG+GGG + chr3_2:26824689-26824708 Msa0401310:CDS 35.0%
GGATATCTTGCAGTTATTAG+AGG + chr3_2:26824765-26824784 Msa0401310:CDS 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG + chr3_2:26824772-26824791 Msa0401310:CDS 35.0%
GAGAGCTATTATGACATGAA+GGG + chr3_2:26824894-26824913 Msa0401310:intron 35.0%
! TCTTTGGCCAAAACGATTTT+AGG - chr3_2:26825133-26825152 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG + chr3_2:26825318-26825337 Msa0401310:intron 35.0%
GAATGTTTAACAGTCTCTCA+GGG - chr3_2:26825489-26825508 None:intergenic 35.0%
TGAATGTTTAACAGTCTCTC+AGG - chr3_2:26825490-26825509 None:intergenic 35.0%
ACTTCAGTCATCTATACCTT+CGG - chr3_2:26825549-26825568 None:intergenic 35.0%
CCAACTAGCTCAATTCTGTA+AGG - chr3_2:26824732-26824751 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG + chr3_2:26824729-26824748 Msa0401310:CDS 40.0%
GGAGAGCTATTATGACATGA+AGG + chr3_2:26824893-26824912 Msa0401310:intron 40.0%
! GTTGCTCCAGTTTTTACCTT+TGG - chr3_2:26825036-26825055 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG + chr3_2:26825138-26825157 Msa0401310:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG - chr3_2:26825149-26825168 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG + chr3_2:26825641-26825660 Msa0401310:intron 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG - chr3_2:26825674-26825693 None:intergenic 40.0%
CCAACTAGCTCAATTCTGTA+AGG - chr3_2:26824732-26824751 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG + chr3_2:26824729-26824748 Msa0401310:CDS 40.0%
GGAGAGCTATTATGACATGA+AGG + chr3_2:26824893-26824912 Msa0401310:intron 40.0%
! GTTGCTCCAGTTTTTACCTT+TGG - chr3_2:26825036-26825055 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG + chr3_2:26825138-26825157 Msa0401310:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG - chr3_2:26825149-26825168 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG + chr3_2:26825641-26825660 Msa0401310:intron 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG - chr3_2:26825674-26825693 None:intergenic 40.0%
! CAGAATTGAGCTAGTTGGTG+TGG + chr3_2:26824734-26824753 Msa0401310:CDS 45.0%
! CTAGTTGGTGTGGTTTGTGA+TGG + chr3_2:26824744-26824763 Msa0401310:CDS 45.0%
TTATGACATGAAGGGCAGTG+AGG + chr3_2:26824902-26824921 Msa0401310:intron 45.0%
TATGACATGAAGGGCAGTGA+GGG + chr3_2:26824903-26824922 Msa0401310:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG + chr3_2:26824980-26824999 Msa0401310:CDS 45.0%
CTCTATTGCACAGATGCTGT+TGG + chr3_2:26825003-26825022 Msa0401310:CDS 45.0%
TATTGCACAGATGCTGTTGG+TGG + chr3_2:26825006-26825025 Msa0401310:CDS 45.0%
GGTCCACCAAAGGTAAAAAC+TGG + chr3_2:26825027-26825046 Msa0401310:intron 45.0%
! GCTCCAGTTTTTACCTTTGG+TGG - chr3_2:26825033-26825052 None:intergenic 45.0%
!! AGTCTCTCAGGGTTGATACA+TGG - chr3_2:26825478-26825497 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG + chr3_2:26825569-26825588 Msa0401310:intron 45.0%
CGCTTCTGCAGATTCAATAG+AGG - chr3_2:26825643-26825662 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG - chr3_2:26825675-26825694 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG + chr3_2:26825693-26825712 Msa0401310:CDS 45.0%
! CAGAATTGAGCTAGTTGGTG+TGG + chr3_2:26824734-26824753 Msa0401310:CDS 45.0%
! CTAGTTGGTGTGGTTTGTGA+TGG + chr3_2:26824744-26824763 Msa0401310:CDS 45.0%
TTATGACATGAAGGGCAGTG+AGG + chr3_2:26824902-26824921 Msa0401310:intron 45.0%
TATGACATGAAGGGCAGTGA+GGG + chr3_2:26824903-26824922 Msa0401310:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG + chr3_2:26824980-26824999 Msa0401310:CDS 45.0%
CTCTATTGCACAGATGCTGT+TGG + chr3_2:26825003-26825022 Msa0401310:CDS 45.0%
TATTGCACAGATGCTGTTGG+TGG + chr3_2:26825006-26825025 Msa0401310:CDS 45.0%
GGTCCACCAAAGGTAAAAAC+TGG + chr3_2:26825027-26825046 Msa0401310:intron 45.0%
! GCTCCAGTTTTTACCTTTGG+TGG - chr3_2:26825033-26825052 None:intergenic 45.0%
!! AGTCTCTCAGGGTTGATACA+TGG - chr3_2:26825478-26825497 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG + chr3_2:26825569-26825588 Msa0401310:intron 45.0%
CGCTTCTGCAGATTCAATAG+AGG - chr3_2:26825643-26825662 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG - chr3_2:26825675-26825694 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG + chr3_2:26825693-26825712 Msa0401310:CDS 45.0%
!! TGCTGTTGGTGGTCCACCAA+AGG + chr3_2:26825017-26825036 Msa0401310:CDS 55.0%
!! TGCTGTTGGTGGTCCACCAA+AGG + chr3_2:26825017-26825036 Msa0401310:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 26824609 26825732 26824609 ID=Msa0401310;Name=Msa0401310
chr3_2 mRNA 26824609 26825732 26824609 ID=Msa0401310-mRNA-1;Parent=Msa0401310;Name=Msa0401310-mRNA-1;_AED=0.19;_eAED=0.53;_QI=0|0|0|0.66|1|1|3|0|127
chr3_2 exon 26824609 26824793 26824609 ID=Msa0401310-mRNA-1:exon:5586;Parent=Msa0401310-mRNA-1
chr3_2 exon 26824915 26825038 26824915 ID=Msa0401310-mRNA-1:exon:5587;Parent=Msa0401310-mRNA-1
chr3_2 exon 26825658 26825732 26825658 ID=Msa0401310-mRNA-1:exon:5588;Parent=Msa0401310-mRNA-1
chr3_2 CDS 26824609 26824793 26824609 ID=Msa0401310-mRNA-1:cds;Parent=Msa0401310-mRNA-1
chr3_2 CDS 26824915 26825038 26824915 ID=Msa0401310-mRNA-1:cds;Parent=Msa0401310-mRNA-1
chr3_2 CDS 26825658 26825732 26825658 ID=Msa0401310-mRNA-1:cds;Parent=Msa0401310-mRNA-1
Gene Sequence

>Msa0401310

ATGATTATTCAAACTATACATGCATTTTTTGTGAAAGGAAACTATACAAAGCAAGTGAATGAGGGAGAAGAAGAACATCAAGAAGAGGAAAATTCTACAGGGGAACTACACTCTCAAAAGCCTTACAGAATTGAGCTAGTTGGTGTGGTTTGTGATGGATATCTTGCAGTTATTAGAGGCATTAGGGCAGTGAGGGTGAATTCACAATTGCAATCTCACAAAAGATTTGCTAATGCATTTCCAAAATATTGCAAACTTGTTGACAGTGCTAGGCTCTATTGCACAGATGCTGTTGGTGGTCCACCAAAGAAGCGGTTAGAGTCCCTAACAGAAGCAGAGCTACAAGTACTGCTTCAAGAAGTCGAGGAAGAGAAGAGTCGTTAG

Protein sequence

>Msa0401310

MIIQTIHAFFVKGNYTKQVNEGEEEHQEEENSTGELHSQKPYRIELVGVVCDGYLAVIRGIRAVRVNSQLQSHKRFANAFPKYCKLVDSARLYCTDAVGGPPKKRLESLTEAELQVLLQEVEEEKSR*