AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0983090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0983090 MtrunA17_Chr1g0187021 88.660 97 3 2 14 103 344 439 6.09e-54 177
Msa0983090 MtrunA17_Chr1g0187071 69.000 100 23 2 11 103 340 438 1.06e-38 136
Msa0983090 MtrunA17_Chr1g0187051 68.041 97 13 2 14 103 339 424 3.51e-37 132
Msa0983090 MtrunA17_Chr7g0260781 69.444 72 15 1 39 103 223 294 6.97e-28 105
Msa0983090 MtrunA17_Chr1g0184551 61.628 86 24 2 25 103 347 430 2.81e-27 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0983090 AT1G04280.2 71.429 77 15 1 34 103 323 399 2.34e-31 116
Msa0983090 AT1G04280.1 71.429 77 15 1 34 103 377 453 4.14e-31 116
Msa0983090 AT1G06750.2 59.794 97 25 3 14 103 349 438 2.63e-29 111
Msa0983090 AT1G06750.3 59.794 97 25 3 14 103 342 431 2.77e-29 111
Msa0983090 AT1G06750.1 59.794 97 25 3 14 103 342 431 2.77e-29 111
Msa0983090 AT2G30630.1 59.794 97 25 3 14 103 356 445 1.02e-28 109
Msa0983090 AT2G30630.2 59.794 97 25 3 14 103 377 466 1.32e-28 109

Find 26 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACAAGTTTGCAATATTT+TGG 0.119289 6_4:+26042113 None:intergenic
CTTGTAGCTCTGCTTCTGTT+AGG 0.288048 6_4:+26041409 None:intergenic
GGATATCTTGCAGTTATTAG+AGG 0.408928 6_4:-26042318 Msa0983090:CDS
TTGCAGTTATTAGAGGCATT+AGG 0.422529 6_4:-26042311 Msa0983090:intron
TTGTAGCTCTGCTTCTGTTA+GGG 0.423721 6_4:+26041410 None:intergenic
CAACAAGAGGAAAATTCTAC+AGG 0.441415 6_4:-26042396 Msa0983090:CDS
CTCTATTGAATCTGCAGAAG+CGG 0.490130 6_4:-26041440 Msa0983090:CDS
CGCTTCTGCAGATTCAATAG+AGG 0.492291 6_4:+26041441 None:intergenic
GCAAACTTGTTGACAGTGCT+AGG 0.509508 6_4:-26042103 Msa0983090:CDS
CTATACAAAGCAAGTGAATG+AGG 0.513355 6_4:-26042433 Msa0983090:CDS
GGAAGAGAAGAGTCGTTAGC+TGG 0.513519 6_4:-26041367 Msa0983090:CDS
CCAACTAGCTCAATTCTGTA+AGG 0.525410 6_4:+26042354 None:intergenic
CCTTACAGAATTGAGCTAGT+TGG 0.526663 6_4:-26042354 Msa0983090:CDS
CAGAATTGAGCTAGTTGGTG+TGG 0.527716 6_4:-26042349 Msa0983090:CDS
CTCTATTGCACAGATGCTGT+TGG 0.532305 6_4:-26042080 Msa0983090:CDS
TATTGCACAGATGCTGTTGG+TGG 0.536088 6_4:-26042077 Msa0983090:CDS
CTAGTTGGTGTGGTTTGCGA+TGG 0.570076 6_4:-26042339 Msa0983090:CDS
TGCTGTTGGTGGTCCACCAA+AGG 0.575982 6_4:-26042066 Msa0983090:intron
TATGACATGAAGGGCAGTGA+GGG 0.577678 6_4:-26042180 Msa0983090:intron
AGAAGAAGAACATCAACAAG+AGG 0.597156 6_4:-26042409 Msa0983090:CDS
AACAAGAGGAAAATTCTACA+GGG 0.606029 6_4:-26042395 Msa0983090:CDS
GTCGTTAGCTGGTAATGAAG+AGG 0.608402 6_4:-26041356 None:intergenic
TATACAAAGCAAGTGAATGA+GGG 0.610525 6_4:-26042432 Msa0983090:CDS
AGTACTGCTTCAAGAAGTCG+AGG 0.646449 6_4:-26041388 Msa0983090:CDS
TTATGACATGAAGGGCAGTG+AGG 0.692934 6_4:-26042181 Msa0983090:intron
ACAAGAGGAAAATTCTACAG+GGG 0.709176 6_4:-26042394 Msa0983090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTACTATATTAATATACTT+TGG - chr6_4:26041561-26041580 Msa0983090:intron 15.0%
!!! TATAATTAACATTTTCCTTT+TGG - chr6_4:26041608-26041627 Msa0983090:intron 15.0%
!!! CTTTTCATTATTGATTTATT+GGG - chr6_4:26042045-26042064 Msa0983090:intron 15.0%
!!! TTTTCATTATTGATTTATTG+GGG - chr6_4:26042046-26042065 Msa0983090:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG + chr6_4:26042120-26042139 None:intergenic 15.0%
!! TCTACTATATTAATATACTT+TGG - chr6_4:26041561-26041580 Msa0983090:intron 15.0%
!!! TATAATTAACATTTTCCTTT+TGG - chr6_4:26041608-26041627 Msa0983090:intron 15.0%
!!! CTTTTCATTATTGATTTATT+GGG - chr6_4:26042045-26042064 Msa0983090:intron 15.0%
!!! TTTTCATTATTGATTTATTG+GGG - chr6_4:26042046-26042065 Msa0983090:intron 15.0%
!!! AATGTAAAACATTTTGAATA+AGG + chr6_4:26042120-26042139 None:intergenic 15.0%
!! ACTATATTAATATACTTTGG+TGG - chr6_4:26041564-26041583 Msa0983090:intron 20.0%
!!! TTGTACTTTTTTATTTCACA+TGG - chr6_4:26041942-26041961 Msa0983090:intron 20.0%
!! CCAATAAATCAATAATGAAA+AGG + chr6_4:26042047-26042066 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAT+TGG - chr6_4:26042044-26042063 Msa0983090:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG + chr6_4:26042116-26042135 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG + chr6_4:26042377-26042396 None:intergenic 20.0%
!! ACTATATTAATATACTTTGG+TGG - chr6_4:26041564-26041583 Msa0983090:intron 20.0%
!!! TTGTACTTTTTTATTTCACA+TGG - chr6_4:26041942-26041961 Msa0983090:intron 20.0%
!! CCAATAAATCAATAATGAAA+AGG + chr6_4:26042047-26042066 None:intergenic 20.0%
!!! CCTTTTCATTATTGATTTAT+TGG - chr6_4:26042044-26042063 Msa0983090:intron 20.0%
!!! TAAAACATTTTGAATAAGGT+TGG + chr6_4:26042116-26042135 None:intergenic 20.0%
!! ATAGAGGTAAAATTATTCTA+TGG + chr6_4:26042377-26042396 None:intergenic 20.0%
!!! ATACATGCATTTTTTGTGAA+AGG - chr6_4:26041372-26041391 Msa0983090:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG - chr6_4:26041620-26041639 Msa0983090:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG + chr6_4:26041721-26041740 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG + chr6_4:26041912-26041931 None:intergenic 25.0%
! CTTATTAGCTCATATATCAA+AGG + chr6_4:26042145-26042164 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG - chr6_4:26042280-26042299 Msa0983090:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG - chr6_4:26042308-26042327 Msa0983090:intron 25.0%
!!! ATACATGCATTTTTTGTGAA+AGG - chr6_4:26041372-26041391 Msa0983090:CDS 25.0%
!!! TTTCCTTTTGGAATTTTAAC+AGG - chr6_4:26041620-26041639 Msa0983090:intron 25.0%
!! TCAACAAGTTTGCAATATTT+TGG + chr6_4:26041721-26041740 None:intergenic 25.0%
! GAATAATATCTTCTGACTTA+CGG + chr6_4:26041912-26041931 None:intergenic 25.0%
! CTTATTAGCTCATATATCAA+AGG + chr6_4:26042145-26042164 None:intergenic 25.0%
! AATGAAATATTTCTTTCCGA+AGG - chr6_4:26042280-26042299 Msa0983090:intron 25.0%
! ATGACTGAAGTTATAAACTT+GGG - chr6_4:26042308-26042327 Msa0983090:intron 25.0%
TATACAAAGCAAGTGAATGA+GGG - chr6_4:26041399-26041418 Msa0983090:CDS 30.0%
AACAAGAGGAAAATTCTACA+GGG - chr6_4:26041436-26041455 Msa0983090:CDS 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG - chr6_4:26041585-26041604 Msa0983090:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG + chr6_4:26041626-26041645 None:intergenic 30.0%
GAGAACTATTATGACATGAA+GGG - chr6_4:26041642-26041661 Msa0983090:intron 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG - chr6_4:26041871-26041890 Msa0983090:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG + chr6_4:26041980-26041999 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG - chr6_4:26042307-26042326 Msa0983090:intron 30.0%
TATACAAAGCAAGTGAATGA+GGG - chr6_4:26041399-26041418 Msa0983090:CDS 30.0%
AACAAGAGGAAAATTCTACA+GGG - chr6_4:26041436-26041455 Msa0983090:CDS 30.0%
!!! GGTGTGATTTTTTGTTATGA+TGG - chr6_4:26041585-26041604 Msa0983090:intron 30.0%
TCTCCTGTTAAAATTCCAAA+AGG + chr6_4:26041626-26041645 None:intergenic 30.0%
GAGAACTATTATGACATGAA+GGG - chr6_4:26041642-26041661 Msa0983090:intron 30.0%
! GTTTTGTCCTAAAATCGTTT+TGG - chr6_4:26041871-26041890 Msa0983090:intron 30.0%
CATTTGTTTGACAAGAACTT+TGG + chr6_4:26041980-26041999 None:intergenic 30.0%
GATGACTGAAGTTATAAACT+TGG - chr6_4:26042307-26042326 Msa0983090:intron 30.0%
CTATACAAAGCAAGTGAATG+AGG - chr6_4:26041398-26041417 Msa0983090:CDS 35.0%
AGAAGAAGAACATCAACAAG+AGG - chr6_4:26041422-26041441 Msa0983090:CDS 35.0%
CAACAAGAGGAAAATTCTAC+AGG - chr6_4:26041435-26041454 Msa0983090:CDS 35.0%
ACAAGAGGAAAATTCTACAG+GGG - chr6_4:26041437-26041456 Msa0983090:CDS 35.0%
GGATATCTTGCAGTTATTAG+AGG - chr6_4:26041513-26041532 Msa0983090:intron 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG - chr6_4:26041520-26041539 Msa0983090:intron 35.0%
GGAGAACTATTATGACATGA+AGG - chr6_4:26041641-26041660 Msa0983090:intron 35.0%
! TCTTTGGCCAAAACGATTTT+AGG + chr6_4:26041881-26041900 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG - chr6_4:26042066-26042085 Msa0983090:intron 35.0%
GAATGTTTAACAGTCTCTCA+GGG + chr6_4:26042237-26042256 None:intergenic 35.0%
TGAATGTTTAACAGTCTCTC+AGG + chr6_4:26042238-26042257 None:intergenic 35.0%
ACTTCAGTCATCTATACCTT+CGG + chr6_4:26042299-26042318 None:intergenic 35.0%
CTATACAAAGCAAGTGAATG+AGG - chr6_4:26041398-26041417 Msa0983090:CDS 35.0%
AGAAGAAGAACATCAACAAG+AGG - chr6_4:26041422-26041441 Msa0983090:CDS 35.0%
CAACAAGAGGAAAATTCTAC+AGG - chr6_4:26041435-26041454 Msa0983090:CDS 35.0%
ACAAGAGGAAAATTCTACAG+GGG - chr6_4:26041437-26041456 Msa0983090:CDS 35.0%
GGATATCTTGCAGTTATTAG+AGG - chr6_4:26041513-26041532 Msa0983090:intron 35.0%
!! TTGCAGTTATTAGAGGCATT+AGG - chr6_4:26041520-26041539 Msa0983090:intron 35.0%
GGAGAACTATTATGACATGA+AGG - chr6_4:26041641-26041660 Msa0983090:intron 35.0%
! TCTTTGGCCAAAACGATTTT+AGG + chr6_4:26041881-26041900 None:intergenic 35.0%
GGGAAATTATTCTTTGTGCT+AGG - chr6_4:26042066-26042085 Msa0983090:intron 35.0%
GAATGTTTAACAGTCTCTCA+GGG + chr6_4:26042237-26042256 None:intergenic 35.0%
TGAATGTTTAACAGTCTCTC+AGG + chr6_4:26042238-26042257 None:intergenic 35.0%
ACTTCAGTCATCTATACCTT+CGG + chr6_4:26042299-26042318 None:intergenic 35.0%
CCAACTAGCTCAATTCTGTA+AGG + chr6_4:26041480-26041499 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG - chr6_4:26041477-26041496 Msa0983090:intron 40.0%
!!! GTTGCTCCTGTTTTTACCTT+TGG + chr6_4:26041784-26041803 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG - chr6_4:26041886-26041905 Msa0983090:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG + chr6_4:26041897-26041916 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG - chr6_4:26042391-26042410 Msa0983090:CDS 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG + chr6_4:26042424-26042443 None:intergenic 40.0%
CCAACTAGCTCAATTCTGTA+AGG + chr6_4:26041480-26041499 None:intergenic 40.0%
CCTTACAGAATTGAGCTAGT+TGG - chr6_4:26041477-26041496 Msa0983090:intron 40.0%
!!! GTTGCTCCTGTTTTTACCTT+TGG + chr6_4:26041784-26041803 None:intergenic 40.0%
!! CGTTTTGGCCAAAGATAATC+AGG - chr6_4:26041886-26041905 Msa0983090:intron 40.0%
ACTTACGGCCTGATTATCTT+TGG + chr6_4:26041897-26041916 None:intergenic 40.0%
CTCTATTGAATCTGCAGAAG+CGG - chr6_4:26042391-26042410 Msa0983090:CDS 40.0%
! TTGTAGCTCTGCTTCTGTTA+GGG + chr6_4:26042424-26042443 None:intergenic 40.0%
! CAGAATTGAGCTAGTTGGTG+TGG - chr6_4:26041482-26041501 Msa0983090:intron 45.0%
TTATGACATGAAGGGCAGTG+AGG - chr6_4:26041650-26041669 Msa0983090:intron 45.0%
TATGACATGAAGGGCAGTGA+GGG - chr6_4:26041651-26041670 Msa0983090:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG - chr6_4:26041728-26041747 Msa0983090:intron 45.0%
CTCTATTGCACAGATGCTGT+TGG - chr6_4:26041751-26041770 Msa0983090:intron 45.0%
TATTGCACAGATGCTGTTGG+TGG - chr6_4:26041754-26041773 Msa0983090:intron 45.0%
GGTCCACCAAAGGTAAAAAC+AGG - chr6_4:26041775-26041794 Msa0983090:intron 45.0%
!!! GCTCCTGTTTTTACCTTTGG+TGG + chr6_4:26041781-26041800 None:intergenic 45.0%
!! AGTCTCTCAGGGTTGATACA+TGG + chr6_4:26042226-26042245 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG - chr6_4:26042319-26042338 Msa0983090:CDS 45.0%
CGCTTCTGCAGATTCAATAG+AGG + chr6_4:26042393-26042412 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG + chr6_4:26042425-26042444 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG - chr6_4:26042443-26042462 Msa0983090:CDS 45.0%
! CAGAATTGAGCTAGTTGGTG+TGG - chr6_4:26041482-26041501 Msa0983090:intron 45.0%
TTATGACATGAAGGGCAGTG+AGG - chr6_4:26041650-26041669 Msa0983090:intron 45.0%
TATGACATGAAGGGCAGTGA+GGG - chr6_4:26041651-26041670 Msa0983090:intron 45.0%
!! GCAAACTTGTTGACAGTGCT+AGG - chr6_4:26041728-26041747 Msa0983090:intron 45.0%
CTCTATTGCACAGATGCTGT+TGG - chr6_4:26041751-26041770 Msa0983090:intron 45.0%
TATTGCACAGATGCTGTTGG+TGG - chr6_4:26041754-26041773 Msa0983090:intron 45.0%
GGTCCACCAAAGGTAAAAAC+AGG - chr6_4:26041775-26041794 Msa0983090:intron 45.0%
!!! GCTCCTGTTTTTACCTTTGG+TGG + chr6_4:26041781-26041800 None:intergenic 45.0%
!! AGTCTCTCAGGGTTGATACA+TGG + chr6_4:26042226-26042245 None:intergenic 45.0%
TATAAACTTGGGTCTGCAGC+AGG - chr6_4:26042319-26042338 Msa0983090:CDS 45.0%
CGCTTCTGCAGATTCAATAG+AGG + chr6_4:26042393-26042412 None:intergenic 45.0%
! CTTGTAGCTCTGCTTCTGTT+AGG + chr6_4:26042425-26042444 None:intergenic 45.0%
AGTACTGCTTCAAGAAGTCG+AGG - chr6_4:26042443-26042462 Msa0983090:CDS 45.0%
! CTAGTTGGTGTGGTTTGCGA+TGG - chr6_4:26041492-26041511 Msa0983090:intron 50.0%
GGAAGAGAAGAGTCGTTAGC+TGG - chr6_4:26042464-26042483 Msa0983090:CDS 50.0%
! CTAGTTGGTGTGGTTTGCGA+TGG - chr6_4:26041492-26041511 Msa0983090:intron 50.0%
GGAAGAGAAGAGTCGTTAGC+TGG - chr6_4:26042464-26042483 Msa0983090:CDS 50.0%
!! TGCTGTTGGTGGTCCACCAA+AGG - chr6_4:26041765-26041784 Msa0983090:intron 55.0%
!! TGCTGTTGGTGGTCCACCAA+AGG - chr6_4:26041765-26041784 Msa0983090:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 26041357 26042496 26041357 ID=Msa0983090;Name=Msa0983090
chr6_4 mRNA 26041357 26042496 26041357 ID=Msa0983090-mRNA-1;Parent=Msa0983090;Name=Msa0983090-mRNA-1;_AED=0.22;_eAED=0.55;_QI=0|0|0|0.66|1|1|3|0|142
chr6_4 exon 26042312 26042496 26042312 ID=Msa0983090-mRNA-1:exon:7359;Parent=Msa0983090-mRNA-1
chr6_4 exon 26042067 26042190 26042067 ID=Msa0983090-mRNA-1:exon:7358;Parent=Msa0983090-mRNA-1
chr6_4 exon 26041357 26041473 26041357 ID=Msa0983090-mRNA-1:exon:7357;Parent=Msa0983090-mRNA-1
chr6_4 CDS 26042312 26042496 26042312 ID=Msa0983090-mRNA-1:cds;Parent=Msa0983090-mRNA-1
chr6_4 CDS 26042067 26042190 26042067 ID=Msa0983090-mRNA-1:cds;Parent=Msa0983090-mRNA-1
chr6_4 CDS 26041357 26041473 26041357 ID=Msa0983090-mRNA-1:cds;Parent=Msa0983090-mRNA-1
Gene Sequence

>Msa0983090

ATGATTATTCAAACTATACATGCATTTTTTGTGAAAGGAAACTATACAAAGCAAGTGAATGAGGGAGAAGAAGAACATCAACAAGAGGAAAATTCTACAGGGGAACTACACTCTCAAAAGCCTTACAGAATTGAGCTAGTTGGTGTGGTTTGCGATGGATATCTTGCAGTTATTAGAGGCATTAGGGCAGTGAGGGTGAATTCACAATTGCAATCTAACAAAAGATTTGCTAATGCATTTCCAAAATATTGCAAACTTGTTGACAGTGCTAGGCTCTATTGCACAGATGCTGTTGGTGGTCCACCAAAGAATAATTTTACCTCTATTGAATCTGCAGAAGCGGTTAGAGTCCCTAACAGAAGCAGAGCTACAAGTACTGCTTCAAGAAGTCGAGGAAGAGAAGAGTCGTTAGCTGGTAATGAAGAG

Protein sequence

>Msa0983090

MIIQTIHAFFVKGNYTKQVNEGEEEHQQEENSTGELHSQKPYRIELVGVVCDGYLAVIRGIRAVRVNSQLQSNKRFANAFPKYCKLVDSARLYCTDAVGGPPKNNFTSIESAEAVRVPNRSRATSTASRSRGREESLAGNEE