AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0414230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414230 MtrunA17_Chr3g0097601 71.373 255 64 2 1 247 7 260 4.27e-120 344
Msa0414230 MtrunA17_Chr3g0091861 67.382 233 63 2 1 220 1150 1382 1.35e-92 299
Msa0414230 MtrunA17_Chr3g0108111 51.203 291 112 4 1 283 151 419 8.52e-85 266
Msa0414230 MtrunA17_Chr3g0108151 64.069 231 74 2 1 222 973 1203 3.68e-84 275
Msa0414230 MtrunA17_Chr3g0107271 56.627 249 84 7 2 237 7 244 3.79e-84 253
Msa0414230 MtrunA17_Chr3g0107931 62.389 226 77 2 5 222 1232 1457 3.26e-83 273
Msa0414230 MtrunA17_Chr3g0107081 73.988 173 39 3 66 238 34 200 5.95e-81 243
Msa0414230 MtrunA17_Chr3g0096701 64.734 207 52 4 66 253 39 243 8.02e-80 256
Msa0414230 MtrunA17_Chr3g0108011 61.712 222 72 1 1 222 1237 1445 1.47e-79 262
Msa0414230 MtrunA17_Chr3g0108071 61.712 222 83 1 1 222 350 569 5.63e-79 257
Msa0414230 MtrunA17_Chr3g0108821 60.909 220 85 1 1 219 1199 1418 1.40e-76 253
Msa0414230 MtrunA17_Chr3g0108171 48.867 309 82 7 1 234 1070 1377 8.70e-76 251
Msa0414230 MtrunA17_Chr3g0105601 48.954 239 109 4 7 233 238 475 3.07e-68 220
Msa0414230 MtrunA17_Chr3g0105551 51.327 226 95 3 31 241 1141 1366 4.83e-68 229
Msa0414230 MtrunA17_Chr3g0105481 54.187 203 82 2 31 222 1055 1257 9.04e-65 219
Msa0414230 MtrunA17_Chr3g0105501 53.731 201 83 3 38 232 1 197 2.08e-64 201
Msa0414230 MtrunA17_Chr3g0105521 53.271 214 88 3 31 233 1023 1235 3.45e-62 212
Msa0414230 MtrunA17_Chr3g0108811 51.961 204 75 4 71 251 1400 1603 3.91e-55 192
Msa0414230 MtrunA17_Chr3g0108811 52.660 188 87 2 1 186 1220 1407 8.84e-52 182
Msa0414230 MtrunA17_Chr3g0107201 83.000 100 16 1 135 234 1 99 6.59e-50 174
Msa0414230 MtrunA17_Chr2g0288311 66.667 117 36 2 78 191 30 146 1.42e-41 141
Msa0414230 MtrunA17_Chr3g0096801 67.619 105 33 1 135 238 1 105 4.89e-41 139
Msa0414230 MtrunA17_Chr1g0160951 34.802 227 134 4 8 220 1005 1231 1.84e-39 146
Msa0414230 MtrunA17_Chr3g0108091 67.619 105 34 0 118 222 2 106 3.30e-37 138
Msa0414230 MtrunA17_Chr3g0100241 36.170 188 104 6 31 203 4 190 9.95e-23 93.6
Msa0414230 MtrunA17_Chr5g0439121 30.909 220 135 6 42 245 1326 1544 1.58e-22 97.4
Msa0414230 MtrunA17_Chr5g0443761 30.275 218 133 4 12 210 865 1082 1.41e-20 91.7
Msa0414230 MtrunA17_Chr3g0100261 34.839 155 89 3 61 203 1054 1208 2.90e-18 85.1
Msa0414230 MtrunA17_Chr3g0099591 29.814 161 101 3 42 190 1025 1185 4.56e-15 75.5
Msa0414230 MtrunA17_Chr3g0106021 46.053 76 41 0 1 76 307 382 1.35e-14 73.2
Msa0414230 MtrunA17_Chr8g0360991 31.579 133 87 2 61 189 1048 1180 3.31e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAGTCCATGGTAAGAAAA+AGG 0.203769 3_2:+59232177 None:intergenic
CATACCAAGGACTAGATTTA+TGG 0.234752 3_2:-59232235 Msa0414230:CDS
CCTTACTAAGATGAAAATTA+AGG 0.242841 3_2:-59232774 Msa0414230:CDS
AATGCTACAAATTTCAGATT+TGG 0.274726 3_2:+59232446 None:intergenic
GCATATGATTGAAGATGATT+TGG 0.365214 3_2:-59232657 Msa0414230:CDS
CTCAGGAAGCTCTTTACATA+TGG 0.410794 3_2:+59232550 None:intergenic
AGCTCTTTACATATGGAGAT+TGG 0.415026 3_2:+59232557 None:intergenic
CTTACTAAGATGAAAATTAA+GGG 0.432382 3_2:-59232773 Msa0414230:CDS
AAGCGAGCGGGAGATGAACT+TGG 0.443895 3_2:-59232879 Msa0414230:CDS
GAAAATGAATTCCTTGGGTC+TGG 0.445836 3_2:+59232402 None:intergenic
CCTTGGGTCTGGCAGAGGCT+TGG 0.460300 3_2:+59232413 None:intergenic
GGGTCTGGCAGAGGCTTGGT+AGG 0.462103 3_2:+59232417 None:intergenic
GAAATATTTGTATGTGAAGA+AGG 0.464163 3_2:-59232488 Msa0414230:CDS
GAATTCCTTGGGTCTGGCAG+AGG 0.478677 3_2:+59232408 None:intergenic
CTGCCTGAAAATGAATTCCT+TGG 0.484708 3_2:+59232396 None:intergenic
TGTCACTTTGGAAAGGTTGA+AGG 0.496737 3_2:-59232945 Msa0414230:CDS
ATCACTAGAATATCTAGCTC+AGG 0.500841 3_2:+59232533 None:intergenic
TGCCTGAAAATGAATTCCTT+GGG 0.502787 3_2:+59232397 None:intergenic
ACATCCATAAATCTAGTCCT+TGG 0.506865 3_2:+59232231 None:intergenic
ACAACATATACTTTCAACAT+TGG 0.523661 3_2:+59232913 None:intergenic
AAGAGAAGCAGATGAGCTAG+AGG 0.571056 3_2:-59232510 Msa0414230:CDS
TCTCAGTGAAGTAAGCGAGC+GGG 0.600009 3_2:-59232891 Msa0414230:CDS
ATTTGTGGCAGGAATCGAGC+GGG 0.601606 3_2:-59232982 Msa0414230:intron
TTATGTGAAGCAACTGCGGT+AGG 0.610675 3_2:+59232705 None:intergenic
AGCGAGCGGGAGATGAACTT+GGG 0.611801 3_2:-59232878 Msa0414230:CDS
CCAAGCCTCTGCCAGACCCA+AGG 0.615768 3_2:-59232413 Msa0414230:CDS
GTCTCAGTGAAGTAAGCGAG+CGG 0.651731 3_2:-59232892 Msa0414230:CDS
ATTATTATGTGAAGCAACTG+CGG 0.662413 3_2:+59232701 None:intergenic
CATTTGTGGCAGGAATCGAG+CGG 0.704258 3_2:-59232983 Msa0414230:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTACTAAGATGAAAATTAA+GGG - chr3_2:59232450-59232469 Msa0414230:CDS 20.0%
!! CTTACTAAGATGAAAATTAA+GGG - chr3_2:59232450-59232469 Msa0414230:CDS 20.0%
! ATTTGTTTCCTATTCATTTG+TGG - chr3_2:59232226-59232245 Msa0414230:CDS 25.0%
! ACAACATATACTTTCAACAT+TGG + chr3_2:59232313-59232332 None:intergenic 25.0%
! TTAAAAAGACATGTCATCAT+AGG + chr3_2:59232399-59232418 None:intergenic 25.0%
!! TATGATGACATGTCTTTTTA+AGG - chr3_2:59232398-59232417 Msa0414230:CDS 25.0%
!! ATGATGACATGTCTTTTTAA+GGG - chr3_2:59232399-59232418 Msa0414230:CDS 25.0%
!! CCTTAATTTTCATCTTAGTA+AGG + chr3_2:59232452-59232471 None:intergenic 25.0%
! CCTTACTAAGATGAAAATTA+AGG - chr3_2:59232449-59232468 Msa0414230:CDS 25.0%
! AATTAAGGGATGTGAAAAAT+TGG - chr3_2:59232464-59232483 Msa0414230:CDS 25.0%
! GAAATATTTGTATGTGAAGA+AGG - chr3_2:59232735-59232754 Msa0414230:CDS 25.0%
! AATGCTACAAATTTCAGATT+TGG + chr3_2:59232780-59232799 None:intergenic 25.0%
!! TCACTCTTTTTAACGATTAT+TGG - chr3_2:59232922-59232941 Msa0414230:CDS 25.0%
!! TATAATTCTTTTGTAGTCCA+TGG + chr3_2:59233060-59233079 None:intergenic 25.0%
! ATTTGTTTCCTATTCATTTG+TGG - chr3_2:59232226-59232245 Msa0414230:CDS 25.0%
! ACAACATATACTTTCAACAT+TGG + chr3_2:59232313-59232332 None:intergenic 25.0%
! TTAAAAAGACATGTCATCAT+AGG + chr3_2:59232399-59232418 None:intergenic 25.0%
!! TATGATGACATGTCTTTTTA+AGG - chr3_2:59232398-59232417 Msa0414230:CDS 25.0%
!! ATGATGACATGTCTTTTTAA+GGG - chr3_2:59232399-59232418 Msa0414230:CDS 25.0%
!! CCTTAATTTTCATCTTAGTA+AGG + chr3_2:59232452-59232471 None:intergenic 25.0%
! CCTTACTAAGATGAAAATTA+AGG - chr3_2:59232449-59232468 Msa0414230:CDS 25.0%
! AATTAAGGGATGTGAAAAAT+TGG - chr3_2:59232464-59232483 Msa0414230:CDS 25.0%
! GAAATATTTGTATGTGAAGA+AGG - chr3_2:59232735-59232754 Msa0414230:CDS 25.0%
! AATGCTACAAATTTCAGATT+TGG + chr3_2:59232780-59232799 None:intergenic 25.0%
!! TCACTCTTTTTAACGATTAT+TGG - chr3_2:59232922-59232941 Msa0414230:CDS 25.0%
!! TATAATTCTTTTGTAGTCCA+TGG + chr3_2:59233060-59233079 None:intergenic 25.0%
! CATCTTAGTAAGGTTTTTGA+GGG + chr3_2:59232442-59232461 None:intergenic 30.0%
! TCATCTTAGTAAGGTTTTTG+AGG + chr3_2:59232443-59232462 None:intergenic 30.0%
ATTATTATGTGAAGCAACTG+CGG + chr3_2:59232525-59232544 None:intergenic 30.0%
GCATATGATTGAAGATGATT+TGG - chr3_2:59232566-59232585 Msa0414230:CDS 30.0%
GTTAAAAAGAGTGAAACAAC+TGG + chr3_2:59232916-59232935 None:intergenic 30.0%
! CTTTTTAACGATTATTGGCA+AGG - chr3_2:59232927-59232946 Msa0414230:CDS 30.0%
ACAACAACAAAAACATACCA+AGG - chr3_2:59232975-59232994 Msa0414230:intron 30.0%
! TTTAACCCTTTTTCTTACCA+TGG - chr3_2:59233040-59233059 Msa0414230:intron 30.0%
! CATCTTAGTAAGGTTTTTGA+GGG + chr3_2:59232442-59232461 None:intergenic 30.0%
! TCATCTTAGTAAGGTTTTTG+AGG + chr3_2:59232443-59232462 None:intergenic 30.0%
ATTATTATGTGAAGCAACTG+CGG + chr3_2:59232525-59232544 None:intergenic 30.0%
GCATATGATTGAAGATGATT+TGG - chr3_2:59232566-59232585 Msa0414230:CDS 30.0%
GTTAAAAAGAGTGAAACAAC+TGG + chr3_2:59232916-59232935 None:intergenic 30.0%
! CTTTTTAACGATTATTGGCA+AGG - chr3_2:59232927-59232946 Msa0414230:CDS 30.0%
ACAACAACAAAAACATACCA+AGG - chr3_2:59232975-59232994 Msa0414230:intron 30.0%
! TTTAACCCTTTTTCTTACCA+TGG - chr3_2:59233040-59233059 Msa0414230:intron 30.0%
GTGAAGAAACAATACTAGCT+TGG + chr3_2:59232186-59232205 None:intergenic 35.0%
!!! AGCATTTTGTCACTTTGGAA+AGG - chr3_2:59232271-59232290 Msa0414230:CDS 35.0%
!! CAACAACTGTTTTGAGCTTT+GGG + chr3_2:59232614-59232633 None:intergenic 35.0%
!! ACAACAACTGTTTTGAGCTT+TGG + chr3_2:59232615-59232634 None:intergenic 35.0%
AGCTCTTTACATATGGAGAT+TGG + chr3_2:59232669-59232688 None:intergenic 35.0%
! ATCACTAGAATATCTAGCTC+AGG + chr3_2:59232693-59232712 None:intergenic 35.0%
TGCCTGAAAATGAATTCCTT+GGG + chr3_2:59232829-59232848 None:intergenic 35.0%
CATACCAAGGACTAGATTTA+TGG - chr3_2:59232988-59233007 Msa0414230:intron 35.0%
ACATCCATAAATCTAGTCCT+TGG + chr3_2:59232995-59233014 None:intergenic 35.0%
GTAGTCCATGGTAAGAAAAA+GGG + chr3_2:59233048-59233067 None:intergenic 35.0%
TGTAGTCCATGGTAAGAAAA+AGG + chr3_2:59233049-59233068 None:intergenic 35.0%
GTGAAGAAACAATACTAGCT+TGG + chr3_2:59232186-59232205 None:intergenic 35.0%
!!! AGCATTTTGTCACTTTGGAA+AGG - chr3_2:59232271-59232290 Msa0414230:CDS 35.0%
!! CAACAACTGTTTTGAGCTTT+GGG + chr3_2:59232614-59232633 None:intergenic 35.0%
!! ACAACAACTGTTTTGAGCTT+TGG + chr3_2:59232615-59232634 None:intergenic 35.0%
AGCTCTTTACATATGGAGAT+TGG + chr3_2:59232669-59232688 None:intergenic 35.0%
! ATCACTAGAATATCTAGCTC+AGG + chr3_2:59232693-59232712 None:intergenic 35.0%
TGCCTGAAAATGAATTCCTT+GGG + chr3_2:59232829-59232848 None:intergenic 35.0%
CATACCAAGGACTAGATTTA+TGG - chr3_2:59232988-59233007 Msa0414230:intron 35.0%
ACATCCATAAATCTAGTCCT+TGG + chr3_2:59232995-59233014 None:intergenic 35.0%
GTAGTCCATGGTAAGAAAAA+GGG + chr3_2:59233048-59233067 None:intergenic 35.0%
TGTAGTCCATGGTAAGAAAA+AGG + chr3_2:59233049-59233068 None:intergenic 35.0%
GTTTCCTATTCATTTGTGGC+AGG - chr3_2:59232230-59232249 Msa0414230:CDS 40.0%
GATTCCTGCCACAAATGAAT+AGG + chr3_2:59232237-59232256 None:intergenic 40.0%
!! TGTGCAGCATTTTGTCACTT+TGG - chr3_2:59232266-59232285 Msa0414230:CDS 40.0%
!! TGTCACTTTGGAAAGGTTGA+AGG - chr3_2:59232278-59232297 Msa0414230:CDS 40.0%
CTCAGGAAGCTCTTTACATA+TGG + chr3_2:59232676-59232695 None:intergenic 40.0%
GAAAATGAATTCCTTGGGTC+TGG + chr3_2:59232824-59232843 None:intergenic 40.0%
! GACCCAAGGAATTCATTTTC+AGG - chr3_2:59232824-59232843 Msa0414230:CDS 40.0%
CTGCCTGAAAATGAATTCCT+TGG + chr3_2:59232830-59232849 None:intergenic 40.0%
GTTTCCTATTCATTTGTGGC+AGG - chr3_2:59232230-59232249 Msa0414230:CDS 40.0%
GATTCCTGCCACAAATGAAT+AGG + chr3_2:59232237-59232256 None:intergenic 40.0%
!! TGTGCAGCATTTTGTCACTT+TGG - chr3_2:59232266-59232285 Msa0414230:CDS 40.0%
!! TGTCACTTTGGAAAGGTTGA+AGG - chr3_2:59232278-59232297 Msa0414230:CDS 40.0%
CTCAGGAAGCTCTTTACATA+TGG + chr3_2:59232676-59232695 None:intergenic 40.0%
GAAAATGAATTCCTTGGGTC+TGG + chr3_2:59232824-59232843 None:intergenic 40.0%
! GACCCAAGGAATTCATTTTC+AGG - chr3_2:59232824-59232843 Msa0414230:CDS 40.0%
CTGCCTGAAAATGAATTCCT+TGG + chr3_2:59232830-59232849 None:intergenic 40.0%
TTATGTGAAGCAACTGCGGT+AGG + chr3_2:59232521-59232540 None:intergenic 45.0%
AAGAGAAGCAGATGAGCTAG+AGG - chr3_2:59232713-59232732 Msa0414230:CDS 45.0%
TTATGTGAAGCAACTGCGGT+AGG + chr3_2:59232521-59232540 None:intergenic 45.0%
AAGAGAAGCAGATGAGCTAG+AGG - chr3_2:59232713-59232732 Msa0414230:CDS 45.0%
CATTTGTGGCAGGAATCGAG+CGG - chr3_2:59232240-59232259 Msa0414230:CDS 50.0%
ATTTGTGGCAGGAATCGAGC+GGG - chr3_2:59232241-59232260 Msa0414230:CDS 50.0%
GTCTCAGTGAAGTAAGCGAG+CGG - chr3_2:59232331-59232350 Msa0414230:CDS 50.0%
TCTCAGTGAAGTAAGCGAGC+GGG - chr3_2:59232332-59232351 Msa0414230:CDS 50.0%
CATTTGTGGCAGGAATCGAG+CGG - chr3_2:59232240-59232259 Msa0414230:CDS 50.0%
ATTTGTGGCAGGAATCGAGC+GGG - chr3_2:59232241-59232260 Msa0414230:CDS 50.0%
GTCTCAGTGAAGTAAGCGAG+CGG - chr3_2:59232331-59232350 Msa0414230:CDS 50.0%
TCTCAGTGAAGTAAGCGAGC+GGG - chr3_2:59232332-59232351 Msa0414230:CDS 50.0%
AAGCGAGCGGGAGATGAACT+TGG - chr3_2:59232344-59232363 Msa0414230:CDS 55.0%
AGCGAGCGGGAGATGAACTT+GGG - chr3_2:59232345-59232364 Msa0414230:CDS 55.0%
GAATTCCTTGGGTCTGGCAG+AGG + chr3_2:59232818-59232837 None:intergenic 55.0%
AAGCGAGCGGGAGATGAACT+TGG - chr3_2:59232344-59232363 Msa0414230:CDS 55.0%
AGCGAGCGGGAGATGAACTT+GGG - chr3_2:59232345-59232364 Msa0414230:CDS 55.0%
GAATTCCTTGGGTCTGGCAG+AGG + chr3_2:59232818-59232837 None:intergenic 55.0%
! GGGTCTGGCAGAGGCTTGGT+AGG + chr3_2:59232809-59232828 None:intergenic 65.0%
CCTTGGGTCTGGCAGAGGCT+TGG + chr3_2:59232813-59232832 None:intergenic 65.0%
CCAAGCCTCTGCCAGACCCA+AGG - chr3_2:59232810-59232829 Msa0414230:CDS 65.0%
! GGGTCTGGCAGAGGCTTGGT+AGG + chr3_2:59232809-59232828 None:intergenic 65.0%
CCTTGGGTCTGGCAGAGGCT+TGG + chr3_2:59232813-59232832 None:intergenic 65.0%
CCAAGCCTCTGCCAGACCCA+AGG - chr3_2:59232810-59232829 Msa0414230:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 59232142 59233103 59232142 ID=Msa0414230;Name=Msa0414230
chr3_2 mRNA 59232142 59233103 59232142 ID=Msa0414230-mRNA-1;Parent=Msa0414230;Name=Msa0414230-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|0.5|1|1|2|0|285
chr3_2 exon 59233098 59233103 59233098 ID=Msa0414230-mRNA-1:exon:12348;Parent=Msa0414230-mRNA-1
chr3_2 exon 59232142 59232993 59232142 ID=Msa0414230-mRNA-1:exon:12347;Parent=Msa0414230-mRNA-1
chr3_2 CDS 59233098 59233103 59233098 ID=Msa0414230-mRNA-1:cds;Parent=Msa0414230-mRNA-1
chr3_2 CDS 59232142 59232993 59232142 ID=Msa0414230-mRNA-1:cds;Parent=Msa0414230-mRNA-1
Gene Sequence

>Msa0414230

ATGGCTGAATCGAGCGGGAATGTGCAGCATTTTGTCACTTTGGAAAGGTTGAAGGTAGATAATTCCAATGTTGAAAGTATATGTTGTCTCAGTGAAGTAAGCGAGCGGGAGATGAACTTGGGATTGCAAAACATTGAGTTATATACTCTACCTATGATGACATGTCTTTTTAAGGGTCTAAAAAAAACTTTTTCCCTCAAAAACCTTACTAAGATGAAAATTAAGGGATGTGAAAAATTGGAAATTGTTTTCTCTACTTCTATAATAATGTGCCTACCGCAGTTGCTTCACATAATAATTAATGACTGCAAAGATTTGAAGCATATGATTGAAGATGATTTGGAGAATAGAAAGACTGTATGCTTCCCAAAGCTCAAAACAGTTGTTGTAGAAAAGTGCAAAATGTTGAAAAACGTCTTTCCAATCTCCATATGTAAAGAGCTTCCTGAGCTAGATATTCTAGTGATAAGAGAAGCAGATGAGCTAGAGGAAATATTTGTATGTGAAGAAGGTGATGAGAAAGTCAATATTCCAAATCTGAAATTTGTAGCATTTGTCAACCTACCAAGCCTCTGCCAGACCCAAGGAATTCATTTTCAGGCAGTACAAAATCGTTTTATACGAAGTTGTCAAGAACTCTCTCTGACTTCAGCAATAACAAATGATGCCAGTTGTTTCACTCTTTTTAACGATTATTGGCAAGGTACACACTATCATACAGTAATATTTACAACAACAAAAACATACCAAGGACTAGATTTATGGATGTTTTCTTTTAAAATTCATCTCTACATTTTAACCCTTTTTCTTACCATGGACTACAAAAGAATTATACACATTTCTGTCAATATCTATTAA

Protein sequence

>Msa0414230

MAESSGNVQHFVTLERLKVDNSNVESICCLSEVSEREMNLGLQNIELYTLPMMTCLFKGLKKTFSLKNLTKMKIKGCEKLEIVFSTSIIMCLPQLLHIIINDCKDLKHMIEDDLENRKTVCFPKLKTVVVEKCKMLKNVFPISICKELPELDILVIREADELEEIFVCEEGDEKVNIPNLKFVAFVNLPSLCQTQGIHFQAVQNRFIRSCQELSLTSAITNDASCFTLFNDYWQGTHYHTVIFTTTKTYQGLDLWMFSFKIHLYILTLFLTMDYKRIIHISVNIY*