AlfalfaGEDB | Msa0414230

M. sativa cultivar zhongmu-4 / Msa0414230

KEGG: K08867
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0414230	XP_039687261.1	86.752	234	30	1	1	234	1215	1447	2.04e-128	409

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0414230	A0A072UWF2	66.182	275	83	2	3	267	15	289	6.51e-117	348

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006110	Msa0414230	0.800187	1.620860e-48	-8.615850e-47
Msa0012060	Msa0414230	0.815123	1.086075e-51	-8.615850e-47
Msa0014710	Msa0414230	0.816930	4.289285e-52	-8.615850e-47
Msa0017510	Msa0414230	0.813738	2.198821e-51	-8.615850e-47
Msa0018650	Msa0414230	0.806270	8.923914e-50	-8.615850e-47
Msa0034110	Msa0414230	0.804818	1.799950e-49	-8.615850e-47
Msa0036140	Msa0414230	0.811093	8.318796e-51	-8.615850e-47
Msa0036920	Msa0414230	0.826814	2.206660e-54	-8.615850e-47
Msa0044260	Msa0414230	0.829318	5.505565e-55	-8.615850e-47
Msa0044330	Msa0414230	0.802158	6.404961e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0414230	MtrunA17_Chr3g0097601	71.373	255	64	2	1	247	7	260	4.27e-120	344
Msa0414230	MtrunA17_Chr3g0091861	67.382	233	63	2	1	220	1150	1382	1.35e-92	299
Msa0414230	MtrunA17_Chr3g0108111	51.203	291	112	4	1	283	151	419	8.52e-85	266
Msa0414230	MtrunA17_Chr3g0108151	64.069	231	74	2	1	222	973	1203	3.68e-84	275
Msa0414230	MtrunA17_Chr3g0107271	56.627	249	84	7	2	237	7	244	3.79e-84	253
Msa0414230	MtrunA17_Chr3g0107931	62.389	226	77	2	5	222	1232	1457	3.26e-83	273
Msa0414230	MtrunA17_Chr3g0107081	73.988	173	39	3	66	238	34	200	5.95e-81	243
Msa0414230	MtrunA17_Chr3g0096701	64.734	207	52	4	66	253	39	243	8.02e-80	256
Msa0414230	MtrunA17_Chr3g0108011	61.712	222	72	1	1	222	1237	1445	1.47e-79	262
Msa0414230	MtrunA17_Chr3g0108071	61.712	222	83	1	1	222	350	569	5.63e-79	257

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 29 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGAAGCAGATGAGCTAG+AGG	0.571056	3_2:-59232510	Msa0414230:CDS
AAGCGAGCGGGAGATGAACT+TGG	0.443895	3_2:-59232879	Msa0414230:CDS
AATGCTACAAATTTCAGATT+TGG	0.274726	3_2:+59232446	None:intergenic
ACAACATATACTTTCAACAT+TGG	0.523661	3_2:+59232913	None:intergenic
ACATCCATAAATCTAGTCCT+TGG	0.506865	3_2:+59232231	None:intergenic
AGCGAGCGGGAGATGAACTT+GGG	0.611801	3_2:-59232878	Msa0414230:CDS
AGCTCTTTACATATGGAGAT+TGG	0.415026	3_2:+59232557	None:intergenic
ATCACTAGAATATCTAGCTC+AGG	0.500841	3_2:+59232533	None:intergenic
ATTATTATGTGAAGCAACTG+CGG	0.662413	3_2:+59232701	None:intergenic
ATTTGTGGCAGGAATCGAGC+GGG	0.601606	3_2:-59232982	Msa0414230:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTATTATGTGAAGCAACTG+CGG	+	chr3_2:59232525-59232544	None:intergenic	30.0%
GCATATGATTGAAGATGATT+TGG	-	chr3_2:59232566-59232585	Msa0414230:CDS	30.0%
GTTAAAAAGAGTGAAACAAC+TGG	+	chr3_2:59232916-59232935	None:intergenic	30.0%
ACAACAACAAAAACATACCA+AGG	-	chr3_2:59232975-59232994	Msa0414230:intron	30.0%
ATTATTATGTGAAGCAACTG+CGG	+	chr3_2:59232525-59232544	None:intergenic	30.0%
GCATATGATTGAAGATGATT+TGG	-	chr3_2:59232566-59232585	Msa0414230:CDS	30.0%
GTTAAAAAGAGTGAAACAAC+TGG	+	chr3_2:59232916-59232935	None:intergenic	30.0%
ACAACAACAAAAACATACCA+AGG	-	chr3_2:59232975-59232994	Msa0414230:intron	30.0%
GTGAAGAAACAATACTAGCT+TGG	+	chr3_2:59232186-59232205	None:intergenic	35.0%
AGCTCTTTACATATGGAGAT+TGG	+	chr3_2:59232669-59232688	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	59232142	59233103	59232142	ID=Msa0414230;Name=Msa0414230
chr3_2	mRNA	59232142	59233103	59232142	ID=Msa0414230-mRNA-1;Parent=Msa0414230;Name=Msa0414230-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|285
chr3_2	exon	59233098	59233103	59233098	ID=Msa0414230-mRNA-1:exon:12348;Parent=Msa0414230-mRNA-1
chr3_2	exon	59232142	59232993	59232142	ID=Msa0414230-mRNA-1:exon:12347;Parent=Msa0414230-mRNA-1
chr3_2	CDS	59233098	59233103	59233098	ID=Msa0414230-mRNA-1:cds;Parent=Msa0414230-mRNA-1
chr3_2	CDS	59232142	59232993	59232142	ID=Msa0414230-mRNA-1:cds;Parent=Msa0414230-mRNA-1

Gene Sequence

>Msa0414230

ATGGCTGAATCGAGCGGGAATGTGCAGCATTTTGTCACTTTGGAAAGGTTGAAGGTAGATAATTCCAATGTTGAAAGTATATGTTGTCTCAGTGAAGTAAGCGAGCGGGAGATGAACTTGGGATTGCAAAACATTGAGTTATATACTCTACCTATGATGACATGTCTTTTTAAGGGTCTAAAAAAAACTTTTTCCCTCAAAAACCTTACTAAGATGAAAATTAAGGGATGTGAAAAATTGGAAATTGTTTTCTCTACTTCTATAATAATGTGCCTACCGCAGTTGCTTCACATAATAATTAATGACTGCAAAGATTTGAAGCATATGATTGAAGATGATTTGGAGAATAGAAAGACTGTATGCTTCCCAAAGCTCAAAACAGTTGTTGTAGAAAAGTGCAAAATGTTGAAAAACGTCTTTCCAATCTCCATATGTAAAGAGCTTCCTGAGCTAGATATTCTAGTGATAAGAGAAGCAGATGAGCTAGAGGAAATATTTGTATGTGAAGAAGGTGATGAGAAAGTCAATATTCCAAATCTGAAATTTGTAGCATTTGTCAACCTACCAAGCCTCTGCCAGACCCAAGGAATTCATTTTCAGGCAGTACAAAATCGTTTTATACGAAGTTGTCAAGAACTCTCTCTGACTTCAGCAATAACAAATGATGCCAGTTGTTTCACTCTTTTTAACGATTATTGGCAAGGTACACACTATCATACAGTAATATTTACAACAACAAAAACATACCAAGGACTAGATTTATGGATGTTTTCTTTTAAAATTCATCTCTACATTTTAACCCTTTTTCTTACCATGGACTACAAAAGAATTATACACATTTCTGTCAATATCTATTAA

Protein sequence

>Msa0414230

MAESSGNVQHFVTLERLKVDNSNVESICCLSEVSEREMNLGLQNIELYTLPMMTCLFKGLKKTFSLKNLTKMKIKGCEKLEIVFSTSIIMCLPQLLHIIINDCKDLKHMIEDDLENRKTVCFPKLKTVVVEKCKMLKNVFPISICKELPELDILVIREADELEEIFVCEEGDEKVNIPNLKFVAFVNLPSLCQTQGIHFQAVQNRFIRSCQELSLTSAITNDASCFTLFNDYWQGTHYHTVIFTTTKTYQGLDLWMFSFKIHLYILTLFLTMDYKRIIHISVNIY*