Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1137690 | XP_006574149.1 | 63.636 | 143 | 29 | 3 | 1 | 120 | 23 | 165 | 1.97e-48 | 163 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1137690 | sp|O82368|Y2988_ARATH | 37.097 | 124 | 62 | 2 | 2 | 109 | 25 | 148 | 2.59e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1137690 | A0A0R0F8P9 | 67.647 | 136 | 28 | 3 | 1 | 120 | 23 | 158 | 4.92e-49 | 168 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016570 | Msa1137690 | 0.803194 | 3.916000e-49 | -8.615850e-47 |
| Msa0016580 | Msa1137690 | 0.835564 | 1.559672e-56 | -8.615850e-47 |
| Msa0044260 | Msa1137690 | 0.804093 | 2.548305e-49 | -8.615850e-47 |
| Msa0110270 | Msa1137690 | 0.801846 | 7.425175e-49 | -8.615850e-47 |
| Msa0639610 | Msa1137690 | 0.808005 | 3.830517e-50 | -8.615850e-47 |
| Msa0657970 | Msa1137690 | 0.801006 | 1.103908e-48 | -8.615850e-47 |
| Msa0678930 | Msa1137690 | 0.802402 | 5.706102e-49 | -8.615850e-47 |
| Msa0688270 | Msa1137690 | 0.801556 | 8.518104e-49 | -8.615850e-47 |
| Msa0697060 | Msa1137690 | 0.815435 | 9.257915e-52 | -8.615850e-47 |
| Msa0726140 | Msa1137690 | 0.837241 | 5.838201e-57 | -8.615850e-47 |
| Msa0729800 | Msa1137690 | 0.812699 | 3.716531e-51 | -8.615850e-47 |
| Msa0731940 | Msa1137690 | 0.800417 | 1.455807e-48 | -8.615850e-47 |
| Msa0733290 | Msa1137690 | 0.805122 | 1.554758e-49 | -8.615850e-47 |
| Msa0735170 | Msa1137690 | 0.808351 | 3.232502e-50 | -8.615850e-47 |
| Msa0741570 | Msa1137690 | 0.803907 | 2.786402e-49 | -8.615850e-47 |
| Msa0760130 | Msa1137690 | 0.806993 | 6.278552e-50 | -8.615850e-47 |
| Msa0762360 | Msa1137690 | 0.800451 | 1.432342e-48 | -8.615850e-47 |
| Msa0766630 | Msa1137690 | 0.868228 | 7.632974e-66 | -8.615850e-47 |
| Msa0777790 | Msa1137690 | 0.806645 | 7.438046e-50 | -8.615850e-47 |
| Msa0783160 | Msa1137690 | 0.807388 | 5.180622e-50 | -8.615850e-47 |
| Msa0811080 | Msa1137690 | 0.810912 | 9.102581e-51 | -8.615850e-47 |
| Msa0822650 | Msa1137690 | 0.802147 | 6.438696e-49 | -8.615850e-47 |
| Msa0825860 | Msa1137690 | 0.803401 | 3.548124e-49 | -8.615850e-47 |
| Msa0840740 | Msa1137690 | 0.835866 | 1.307396e-56 | -8.615850e-47 |
| Msa0131770 | Msa1137690 | 0.805553 | 1.262906e-49 | -8.615850e-47 |
| Msa0142220 | Msa1137690 | 0.850137 | 2.062841e-60 | -8.615850e-47 |
| Msa0153910 | Msa1137690 | 0.813770 | 2.163430e-51 | -8.615850e-47 |
| Msa0158480 | Msa1137690 | 0.803081 | 4.132954e-49 | -8.615850e-47 |
| Msa0208090 | Msa1137690 | 0.801515 | 8.683455e-49 | -8.615850e-47 |
| Msa0227480 | Msa1137690 | 0.804344 | 2.259746e-49 | -8.615850e-47 |
| Msa0231000 | Msa1137690 | 0.805136 | 1.544363e-49 | -8.615850e-47 |
| Msa0233300 | Msa1137690 | 0.811531 | 6.682884e-51 | -8.615850e-47 |
| Msa0384800 | Msa1137690 | 0.838598 | 2.614985e-57 | -8.615850e-47 |
| Msa0384890 | Msa1137690 | 0.840539 | 8.181452e-58 | -8.615850e-47 |
| Msa0390070 | Msa1137690 | 0.801051 | 1.080739e-48 | -8.615850e-47 |
| Msa0394970 | Msa1137690 | 0.812982 | 3.222378e-51 | -8.615850e-47 |
| Msa0402290 | Msa1137690 | 0.837121 | 6.264834e-57 | -8.615850e-47 |
| Msa0408110 | Msa1137690 | 0.820865 | 5.473276e-53 | -8.615850e-47 |
| Msa0435230 | Msa1137690 | 0.837786 | 4.231523e-57 | -8.615850e-47 |
| Msa0436800 | Msa1137690 | 0.819924 | 8.996619e-53 | -8.615850e-47 |
| Msa0452620 | Msa1137690 | 0.813343 | 2.685011e-51 | -8.615850e-47 |
| Msa0453120 | Msa1137690 | 0.801675 | 8.050462e-49 | -8.615850e-47 |
| Msa0479340 | Msa1137690 | 0.854606 | 1.100492e-61 | -8.615850e-47 |
| Msa0981370 | Msa1137690 | 0.803980 | 2.691007e-49 | -8.615850e-47 |
| Msa0983090 | Msa1137690 | 0.823833 | 1.119722e-53 | -8.615850e-47 |
| Msa0989060 | Msa1137690 | 0.820110 | 8.156281e-53 | -8.615850e-47 |
| Msa0991080 | Msa1137690 | 0.827560 | 1.462292e-54 | -8.615850e-47 |
| Msa0998690 | Msa1137690 | 0.841018 | 6.127175e-58 | -8.615850e-47 |
| Msa1002310 | Msa1137690 | 0.814197 | 1.741627e-51 | -8.615850e-47 |
| Msa1014540 | Msa1137690 | 0.822606 | 2.164530e-53 | -8.615850e-47 |
| Msa1015490 | Msa1137690 | 0.820940 | 5.259921e-53 | -8.615850e-47 |
| Msa1016280 | Msa1137690 | 0.809543 | 1.796743e-50 | -8.615850e-47 |
| Msa1024240 | Msa1137690 | 0.809889 | 1.513566e-50 | -8.615850e-47 |
| Msa1065570 | Msa1137690 | 0.824989 | 5.984965e-54 | -8.615850e-47 |
| Msa1082500 | Msa1137690 | 0.809871 | 1.527487e-50 | -8.615850e-47 |
| Msa1100980 | Msa1137690 | 0.820533 | 6.524451e-53 | -8.615850e-47 |
| Msa0251320 | Msa1137690 | 0.818239 | 2.174036e-52 | -8.615850e-47 |
| Msa0270520 | Msa1137690 | 0.809382 | 1.945175e-50 | -8.615850e-47 |
| Msa0273100 | Msa1137690 | 0.820617 | 6.238227e-53 | -8.615850e-47 |
| Msa0349360 | Msa1137690 | 0.806046 | 9.946801e-50 | -8.615850e-47 |
| Msa0355220 | Msa1137690 | 0.828587 | 8.275492e-55 | -8.615850e-47 |
| Msa0363300 | Msa1137690 | 0.825444 | 4.671232e-54 | -8.615850e-47 |
| Msa1111150 | Msa1137690 | 0.803662 | 3.131716e-49 | -8.615850e-47 |
| Msa1121490 | Msa1137690 | 0.807830 | 4.172057e-50 | -8.615850e-47 |
| Msa1135740 | Msa1137690 | 0.808811 | 2.577914e-50 | -8.615850e-47 |
| Msa1136090 | Msa1137690 | 0.839928 | 1.181247e-57 | -8.615850e-47 |
| Msa1137690 | Msa1160670 | 0.820030 | 8.505483e-53 | -8.615850e-47 |
| Msa1137690 | Msa1162550 | -0.807024 | 6.186160e-50 | -8.615850e-47 |
| Msa1137690 | Msa1187950 | 0.836237 | 1.052584e-56 | -8.615850e-47 |
| Msa1137690 | Msa1195920 | 0.805720 | 1.164921e-49 | -8.615850e-47 |
| Msa1137690 | Msa1244250 | 0.814353 | 1.608789e-51 | -8.615850e-47 |
| Msa1137690 | Msa1249490 | 0.820651 | 6.129482e-53 | -8.615850e-47 |
| Msa1137690 | Msa1269670 | 0.801138 | 1.037212e-48 | -8.615850e-47 |
| Msa1137690 | Msa1270240 | 0.802464 | 5.540670e-49 | -8.615850e-47 |
| Msa1137690 | Msa1273230 | 0.819141 | 1.356923e-52 | -8.615850e-47 |
| Msa1137690 | Msa1283330 | 0.841899 | 3.591000e-58 | -8.615850e-47 |
| Msa1137690 | Msa1284680 | 0.834608 | 2.716677e-56 | -8.615850e-47 |
| Msa1137690 | Msa1285930 | 0.813189 | 2.903752e-51 | -8.615850e-47 |
| Msa1137690 | Msa1297210 | 0.825204 | 5.325776e-54 | -8.615850e-47 |
| Msa1137690 | Msa1340590 | 0.811554 | 6.605830e-51 | -8.615850e-47 |
| Msa1137690 | Msa1382690 | 0.802181 | 6.335805e-49 | -8.615850e-47 |
| Msa1137690 | Msa1397580 | 0.802185 | 6.325954e-49 | -8.615850e-47 |
| Msa1137690 | Msa1397610 | 0.830698 | 2.536515e-55 | -8.615850e-47 |
| Msa1137690 | Msa1397860 | 0.833867 | 4.167470e-56 | -8.615850e-47 |
| Msa1137690 | Msa1400720 | 0.807004 | 6.246688e-50 | -8.615850e-47 |
| Msa1137690 | Msa1404000 | 0.814152 | 1.781725e-51 | -8.615850e-47 |
| Msa1137690 | Msa1422180 | 0.824027 | 1.008093e-53 | -8.615850e-47 |
| Msa0502270 | Msa1137690 | 0.819062 | 1.414163e-52 | -8.615850e-47 |
| Msa0506270 | Msa1137690 | 0.809516 | 1.820349e-50 | -8.615850e-47 |
| Msa0532350 | Msa1137690 | 0.816406 | 5.621645e-52 | -8.615850e-47 |
| Msa0541130 | Msa1137690 | 0.806716 | 7.186407e-50 | -8.615850e-47 |
| Msa0598780 | Msa1137690 | 0.840909 | 6.546382e-58 | -8.615850e-47 |
| Msa0600700 | Msa1137690 | 0.812271 | 4.611767e-51 | -8.615850e-47 |
| Msa0846330 | Msa1137690 | 0.806248 | 9.021118e-50 | -8.615850e-47 |
| Msa0846350 | Msa1137690 | 0.836754 | 7.773875e-57 | -8.615850e-47 |
| Msa0858980 | Msa1137690 | 0.827393 | 1.603780e-54 | -8.615850e-47 |
| Msa0885190 | Msa1137690 | 0.808606 | 2.852041e-50 | -8.615850e-47 |
| Msa0885210 | Msa1137690 | 0.801954 | 7.055941e-49 | -8.615850e-47 |
| Msa0885280 | Msa1137690 | 0.836135 | 1.117138e-56 | -8.615850e-47 |
| Msa0885290 | Msa1137690 | 0.823980 | 1.034221e-53 | -8.615850e-47 |
| Msa0898380 | Msa1137690 | 0.825189 | 5.367606e-54 | -8.615850e-47 |
| Msa0907070 | Msa1137690 | 0.818934 | 1.512756e-52 | -8.615850e-47 |
| Msa0907260 | Msa1137690 | 0.847663 | 1.003065e-59 | -8.615850e-47 |
| Msa0909120 | Msa1137690 | 0.803967 | 2.707504e-49 | -8.615850e-47 |
| Msa0935910 | Msa1137690 | 0.814300 | 1.652629e-51 | -8.615850e-47 |
| Msa0939250 | Msa1137690 | 0.801193 | 1.010843e-48 | -8.615850e-47 |
| Msa0954910 | Msa1137690 | 0.832078 | 1.160283e-55 | -8.615850e-47 |
| Msa0956270 | Msa1137690 | 0.855015 | 8.373689e-62 | -8.615850e-47 |
| Msa0957860 | Msa1137690 | 0.804831 | 1.788044e-49 | -8.615850e-47 |
| Msa0957960 | Msa1137690 | 0.804474 | 2.123300e-49 | -8.615850e-47 |
| Msa0979410 | Msa1137690 | 0.824068 | 9.862451e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1137690 | MtrunA17_Chr4g0051721 | 58.042 | 143 | 37 | 3 | 1 | 120 | 24 | 166 | 8.18e-46 | 147 |
| Msa1137690 | MtrunA17_Chr1g0207381 | 57.343 | 143 | 38 | 3 | 1 | 120 | 24 | 166 | 9.87e-44 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1137690 | AT2G29880.1 | 37.097 | 124 | 62 | 2 | 2 | 109 | 25 | 148 | 2.63e-14 | 67.8 |
Find 13 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTGACATCTTGTTATAT+TGG | 0.307503 | 7_4:+6612848 | None:intergenic |
| GGTTTCTAAACCACTTCATT+TGG | 0.344549 | 7_4:+6612869 | None:intergenic |
| CATTGAATTGTTGCACCTAT+TGG | 0.359106 | 7_4:-6613019 | Msa1137690:CDS |
| CAAAACTATGATTGATTATG+AGG | 0.417510 | 7_4:-6612646 | Msa1137690:CDS |
| TATTTATAGCATCCACCAAT+AGG | 0.450108 | 7_4:+6613004 | None:intergenic |
| GAGGAGTTGAAGAATGTTGT+TGG | 0.455416 | 7_4:-6612627 | Msa1137690:CDS |
| ATGTAGAACAACTCTAGCTT+TGG | 0.462599 | 7_4:-6612825 | Msa1137690:intron |
| CAATAGGTGCAACAATTCAA+TGG | 0.483715 | 7_4:+6613020 | None:intergenic |
| TTAGCAAACAAAATGTAGAA+TGG | 0.507888 | 7_4:-6612954 | Msa1137690:CDS |
| TGAATTGTTGCACCTATTGG+TGG | 0.519657 | 7_4:-6613016 | Msa1137690:CDS |
| TTGGTGGATGCTATAAATAG+AGG | 0.539136 | 7_4:-6613000 | Msa1137690:CDS |
| ATTGCTTGAGCCAAATGAAG+TGG | 0.563981 | 7_4:-6612879 | Msa1137690:CDS |
| TGGTGGATGCTATAAATAGA+GGG | 0.614176 | 7_4:-6612999 | Msa1137690:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGCAATGAATATAAGTTTTA+GGG | + | chr7_4:6612715-6612734 | None:intergenic | 20.0% |
| !!! | AAGCAATGAATATAAGTTTT+AGG | + | chr7_4:6612716-6612735 | None:intergenic | 20.0% |
| ! | TTAGCAAACAAAATGTAGAA+TGG | - | chr7_4:6612654-6612673 | Msa1137690:CDS | 25.0% |
| !! | AAGTTGACATCTTGTTATAT+TGG | + | chr7_4:6612763-6612782 | None:intergenic | 25.0% |
| ! | CAAAACTATGATTGATTATG+AGG | - | chr7_4:6612962-6612981 | Msa1137690:CDS | 25.0% |
| TATTTATAGCATCCACCAAT+AGG | + | chr7_4:6612607-6612626 | None:intergenic | 30.0% | |
| !!! | TTAGTTTTGACATTGAGTTG+AGG | + | chr7_4:6612689-6612708 | None:intergenic | 30.0% |
| !! | AAGTTTGTTGTGACTTTAGT+GGG | + | chr7_4:6612937-6612956 | None:intergenic | 30.0% |
| ! | TTTTTGCATCAGTATCATCA+GGG | + | chr7_4:6613025-6613044 | None:intergenic | 30.0% |
| !!! | TTTTTTGCATCAGTATCATC+AGG | + | chr7_4:6613026-6613045 | None:intergenic | 30.0% |
| CAATAGGTGCAACAATTCAA+TGG | + | chr7_4:6612591-6612610 | None:intergenic | 35.0% | |
| CATTGAATTGTTGCACCTAT+TGG | - | chr7_4:6612589-6612608 | Msa1137690:CDS | 35.0% | |
| ! | TTGGTGGATGCTATAAATAG+AGG | - | chr7_4:6612608-6612627 | Msa1137690:CDS | 35.0% |
| ! | TGGTGGATGCTATAAATAGA+GGG | - | chr7_4:6612609-6612628 | Msa1137690:CDS | 35.0% |
| GGTTTCTAAACCACTTCATT+TGG | + | chr7_4:6612742-6612761 | None:intergenic | 35.0% | |
| ! | ATGTAGAACAACTCTAGCTT+TGG | - | chr7_4:6612783-6612802 | Msa1137690:intron | 35.0% |
| !! | GAAGTTTGTTGTGACTTTAG+TGG | + | chr7_4:6612938-6612957 | None:intergenic | 35.0% |
| TGAATTGTTGCACCTATTGG+TGG | - | chr7_4:6612592-6612611 | Msa1137690:CDS | 40.0% | |
| ATTGCTTGAGCCAAATGAAG+TGG | - | chr7_4:6612729-6612748 | Msa1137690:intron | 40.0% | |
| GCAAAGACTGTGACAAAGTA+TGG | - | chr7_4:6612819-6612838 | Msa1137690:CDS | 40.0% | |
| AGACTGTGACAAAGTATGGA+AGG | - | chr7_4:6612823-6612842 | Msa1137690:intron | 40.0% | |
| GAGGAGTTGAAGAATGTTGT+TGG | - | chr7_4:6612981-6613000 | Msa1137690:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 6612578 | 6613052 | 6612578 | ID=Msa1137690;Name=Msa1137690 |
| chr7_4 | mRNA | 6612578 | 6613052 | 6612578 | ID=Msa1137690-mRNA-1;Parent=Msa1137690;Name=Msa1137690-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|0|0|3|0|120 |
| chr7_4 | exon | 6612846 | 6613052 | 6612846 | ID=Msa1137690-mRNA-1:exon:1862;Parent=Msa1137690-mRNA-1 |
| chr7_4 | exon | 6612795 | 6612841 | 6612795 | ID=Msa1137690-mRNA-1:exon:1861;Parent=Msa1137690-mRNA-1 |
| chr7_4 | exon | 6612578 | 6612686 | 6612578 | ID=Msa1137690-mRNA-1:exon:1860;Parent=Msa1137690-mRNA-1 |
| chr7_4 | CDS | 6612846 | 6613052 | 6612846 | ID=Msa1137690-mRNA-1:cds;Parent=Msa1137690-mRNA-1 |
| chr7_4 | CDS | 6612795 | 6612841 | 6612795 | ID=Msa1137690-mRNA-1:cds;Parent=Msa1137690-mRNA-1 |
| chr7_4 | CDS | 6612578 | 6612686 | 6612578 | ID=Msa1137690-mRNA-1:cds;Parent=Msa1137690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
