AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1129100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1129100 MtrunA17_Chr7g0273641 96.602 206 7 0 1 206 1 206 3.26e-150 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1129100 AT3G04950.1 64.706 187 62 2 23 206 41 226 1.84e-86 257

Find 68 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTGTTAATCTACAATTT+TGG 0.108080 7_3:+81035398 Msa1129100:three_prime_UTR
TAAATTATGCCTTAACAATT+TGG 0.113030 7_3:-81035520 None:intergenic
TCCAACTATATACTCTTTAA+AGG 0.178788 7_3:-81033649 None:intergenic
GGATATTGCAAGGTCTAATT+TGG 0.200253 7_3:+81034532 Msa1129100:CDS
AACAAACTCAAGCAACTATT+TGG 0.211577 7_3:-81035383 None:intergenic
TTCCCTTCAGCCTTAATAAA+AGG 0.215422 7_3:+81035490 Msa1129100:three_prime_UTR
TCTCGGCTTGCTAAAGTTCT+TGG 0.252133 7_3:+81034756 Msa1129100:CDS
CTCGGCTTGCTAAAGTTCTT+GGG 0.291086 7_3:+81034757 Msa1129100:CDS
GATTTGTACCGACCATCTTT+TGG 0.311365 7_3:-81034505 None:intergenic
ATTTGATCCCTAGCGTAATC+TGG 0.335969 7_3:+81035570 Msa1129100:three_prime_UTR
CGGTTCACCTTCAGAGTTCA+TGG 0.336324 7_3:+81033440 Msa1129100:CDS
AAATTATGCCTTAACAATTT+GGG 0.336813 7_3:-81035519 None:intergenic
ATGCACTTGCAAAGTTTACT+TGG 0.358285 7_3:+81033898 Msa1129100:CDS
GGTCATAATAATCAAATGTT+AGG 0.373371 7_3:+81035347 Msa1129100:three_prime_UTR
TGCATCTGGTGGTGCTCCTT+CGG 0.382519 7_3:-81033500 None:intergenic
GTTCAAAGAAGAGATATAAA+AGG 0.388106 7_3:+81034597 Msa1129100:CDS
GAGTTAAAGGTATCGAATAA+TGG 0.404497 7_3:+81033333 Msa1129100:exon
GAAAATGAACTTACGGAGTA+AGG 0.422469 7_3:-81033533 None:intergenic
AAGACTGGGAAGTGGAAAAC+TGG 0.423192 7_3:-81033295 None:intergenic
GGGGAAAAGGCAATTTGGGT+TGG 0.426545 7_3:+81033269 None:intergenic
GTTATGTCCACTTGTTATCT+CGG 0.430472 7_3:+81034739 Msa1129100:CDS
GCCTTTAAAGAGTATATAGT+TGG 0.433265 7_3:+81033648 Msa1129100:CDS
AATCGAATGCGGCGAGATAT+CGG 0.442505 7_3:-81033725 None:intergenic
GTGTAGATCCCATTCAGTTA+AGG 0.446462 7_3:-81035268 None:intergenic
GGCAAGAACCCAAATTGTTA+AGG 0.454046 7_3:+81035511 Msa1129100:three_prime_UTR
TCTTGCTTTCTTCATTTCAT+CGG 0.458556 7_3:-81033616 None:intergenic
ATGAAGAAAGCAAGAAAAGT+TGG 0.472505 7_3:+81033624 Msa1129100:CDS
TTAGACCTTGCAATATCCAT+TGG 0.473307 7_3:-81034527 None:intergenic
GCAAGGAAGCCTTAACTGAA+TGG 0.480468 7_3:+81035259 Msa1129100:CDS
TTTCAAAGCCAGATTACGCT+AGG 0.491492 7_3:-81035578 None:intergenic
TTCTTGTTAGCTCCAAAAGA+TGG 0.497318 7_3:+81034493 Msa1129100:intron
CAAGGAAGCCTTAACTGAAT+GGG 0.501539 7_3:+81035260 Msa1129100:CDS
CTAAAAGCGGACAAGTTAGC+AGG 0.515399 7_3:+81034555 Msa1129100:CDS
AATGCCTTCGTATTTCTCAA+TGG 0.521342 7_3:-81033700 None:intergenic
TGCACTTGCAAAGTTTACTT+GGG 0.524528 7_3:+81033899 Msa1129100:CDS
TTTATGAAGGTGCTGTGGAA+AGG 0.546367 7_3:+81035204 Msa1129100:intron
TAAGTGCACAATTGCTGAAG+TGG 0.546585 7_3:+81033872 Msa1129100:CDS
CCACCGTTCAATTTCCACTA+CGG 0.551541 7_3:+81033420 Msa1129100:CDS
ATTCAAAAGTTAAATCAAGA+AGG 0.556894 7_3:+81033942 Msa1129100:CDS
TCGGCTTGCTAAAGTTCTTG+GGG 0.563252 7_3:+81034758 Msa1129100:CDS
TATCTCTTCTTTGAACCACA+TGG 0.570464 7_3:-81034590 None:intergenic
GATGGTCGGTACAAATCCAA+TGG 0.571490 7_3:+81034511 Msa1129100:CDS
TCACCTTCAGAGTTCATGGA+TGG 0.571753 7_3:+81033444 Msa1129100:CDS
TTCAAAGCCAGATTACGCTA+GGG 0.572480 7_3:-81035577 None:intergenic
CTTTGAACCACATGGACAGA+GGG 0.580056 7_3:-81034582 None:intergenic
ATTTATTTATGAAGGTGCTG+TGG 0.584119 7_3:+81035199 Msa1129100:intron
TAGTGGAAATTGAACGGTGG+TGG 0.584928 7_3:-81033417 None:intergenic
CAAATCCAATGGATATTGCA+AGG 0.593492 7_3:+81034522 Msa1129100:CDS
TGTCCATCCATGAACTCTGA+AGG 0.596448 7_3:-81033447 None:intergenic
CGCATTCGATTACACAGGAG+AGG 0.602676 7_3:+81033737 Msa1129100:CDS
AAGGTGAATTGAGATGCATC+TGG 0.611379 7_3:-81033514 None:intergenic
ACTCTGAAGGTGAACCGTAG+TGG 0.617132 7_3:-81033434 None:intergenic
AATGCACTAGACTGCAGACA+TGG 0.621775 7_3:+81035326 Msa1129100:exon
AGGAATGCCCTCTGTCCATG+TGG 0.628191 7_3:+81034575 Msa1129100:CDS
AGGTCTAATTTGGCTAAAAG+CGG 0.628257 7_3:+81034542 Msa1129100:CDS
AGGACAAGTATTAAACTGCA+AGG 0.629058 7_3:+81035242 Msa1129100:CDS
TGTTAGCTCCAAAAGATGGT+CGG 0.634247 7_3:+81034497 Msa1129100:intron
TAGCAAGCCGAGATAACAAG+TGG 0.636899 7_3:-81034746 None:intergenic
TCTTTGAACCACATGGACAG+AGG 0.637245 7_3:-81034583 None:intergenic
CTCTCCTGTGTAATCGAATG+CGG 0.644799 7_3:-81033736 None:intergenic
GAACCGTAGTGGAAATTGAA+CGG 0.645642 7_3:-81033423 None:intergenic
AAAGGATTGAAAGAATTCAG+AGG 0.649542 7_3:+81035222 Msa1129100:CDS
TCAGAGTTCATGGATGGACA+AGG 0.658450 7_3:+81033450 Msa1129100:CDS
TCTGTTTGCGATGCGAAGTG+CGG 0.662046 7_3:-81033356 None:intergenic
CTGCAGTCTAGTGCATTGAG+CGG 0.670629 7_3:-81035320 None:intergenic
ACTGCCATTGAGAAATACGA+AGG 0.691670 7_3:+81033696 Msa1129100:CDS
GTGAATTGAGATGCATCTGG+TGG 0.748795 7_3:-81033511 None:intergenic
CCGTAGTGGAAATTGAACGG+TGG 0.810192 7_3:-81033420 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTAATAAAATGATGA+AGG - chr7_3:81034022-81034041 None:intergenic 10.0%
!! TAAAATTAATAAAATGATGA+AGG - chr7_3:81034022-81034041 None:intergenic 10.0%
!! TAAAAAATGAAAAAGTTACT+AGG - chr7_3:81034930-81034949 None:intergenic 15.0%
!!! TTTTGTATATTTTCAAAATC+TGG + chr7_3:81034989-81035008 Msa1129100:intron 15.0%
!!! TTTGTATATTTTCAAAATCT+GGG + chr7_3:81034990-81035009 Msa1129100:intron 15.0%
!! AATTCAAATTCAAAACAATT+GGG - chr7_3:81035110-81035129 None:intergenic 15.0%
!! AAATTCAAATTCAAAACAAT+TGG - chr7_3:81035111-81035130 None:intergenic 15.0%
!! TTATGAGTATTTATTTATGA+AGG + chr7_3:81035191-81035210 Msa1129100:intron 15.0%
!! ATTAACATATTTCACAATTT+CGG + chr7_3:81035601-81035620 Msa1129100:three_prime_UTR 15.0%
!! TAAAAAATGAAAAAGTTACT+AGG - chr7_3:81034930-81034949 None:intergenic 15.0%
!!! TTTTGTATATTTTCAAAATC+TGG + chr7_3:81034989-81035008 Msa1129100:intron 15.0%
!!! TTTGTATATTTTCAAAATCT+GGG + chr7_3:81034990-81035009 Msa1129100:intron 15.0%
!! AATTCAAATTCAAAACAATT+GGG - chr7_3:81035110-81035129 None:intergenic 15.0%
!! AAATTCAAATTCAAAACAAT+TGG - chr7_3:81035111-81035130 None:intergenic 15.0%
!! TTATGAGTATTTATTTATGA+AGG + chr7_3:81035191-81035210 Msa1129100:intron 15.0%
!!! TTGTTGATTTTGTTTTGTTA+TGG + chr7_3:81033804-81033823 Msa1129100:intron 20.0%
!! ATTCAAAAGTTAAATCAAGA+AGG + chr7_3:81033942-81033961 Msa1129100:CDS 20.0%
!!! ATTACTTCAGACATATTTTT+AGG - chr7_3:81033978-81033997 None:intergenic 20.0%
!!! GAAGATTTTCTTTTTTTGAA+CGG - chr7_3:81034637-81034656 None:intergenic 20.0%
!!! ATAGAAAAGTACTAAGTTAT+AGG - chr7_3:81034877-81034896 None:intergenic 20.0%
!! ACTAATCTAATATCTATCAA+AGG - chr7_3:81034901-81034920 None:intergenic 20.0%
!! ATTCAAATTCAAAACAATTG+GGG - chr7_3:81035109-81035128 None:intergenic 20.0%
!!! AGTTTGTTAATCTACAATTT+TGG + chr7_3:81035398-81035417 Msa1129100:three_prime_UTR 20.0%
!!! GTTTGTTAATCTACAATTTT+GGG + chr7_3:81035399-81035418 Msa1129100:three_prime_UTR 20.0%
!!! TTTGTTAATCTACAATTTTG+GGG + chr7_3:81035400-81035419 Msa1129100:three_prime_UTR 20.0%
!! AAATTATGCCTTAACAATTT+GGG - chr7_3:81035522-81035541 None:intergenic 20.0%
!! TAAATTATGCCTTAACAATT+TGG - chr7_3:81035523-81035542 None:intergenic 20.0%
!! ATTAAAAAAGTGAGATTACA+GGG - chr7_3:81035548-81035567 None:intergenic 20.0%
!! AATTAAAAAAGTGAGATTAC+AGG - chr7_3:81035549-81035568 None:intergenic 20.0%
!!! TTGTTGATTTTGTTTTGTTA+TGG + chr7_3:81033804-81033823 Msa1129100:intron 20.0%
!! ATTCAAAAGTTAAATCAAGA+AGG + chr7_3:81033942-81033961 Msa1129100:CDS 20.0%
!!! ATTACTTCAGACATATTTTT+AGG - chr7_3:81033978-81033997 None:intergenic 20.0%
!!! GAAGATTTTCTTTTTTTGAA+CGG - chr7_3:81034637-81034656 None:intergenic 20.0%
!!! ATAGAAAAGTACTAAGTTAT+AGG - chr7_3:81034877-81034896 None:intergenic 20.0%
!! ACTAATCTAATATCTATCAA+AGG - chr7_3:81034901-81034920 None:intergenic 20.0%
!! ATTCAAATTCAAAACAATTG+GGG - chr7_3:81035109-81035128 None:intergenic 20.0%
!!! AGTTTGTTAATCTACAATTT+TGG + chr7_3:81035398-81035417 Msa1129100:three_prime_UTR 20.0%
!!! GTTTGTTAATCTACAATTTT+GGG + chr7_3:81035399-81035418 Msa1129100:three_prime_UTR 20.0%
!!! TTTGTTAATCTACAATTTTG+GGG + chr7_3:81035400-81035419 Msa1129100:three_prime_UTR 20.0%
!! AAATTATGCCTTAACAATTT+GGG - chr7_3:81035522-81035541 None:intergenic 20.0%
!! TAAATTATGCCTTAACAATT+TGG - chr7_3:81035523-81035542 None:intergenic 20.0%
!! ATTAAAAAAGTGAGATTACA+GGG - chr7_3:81035548-81035567 None:intergenic 20.0%
!! AATTAAAAAAGTGAGATTAC+AGG - chr7_3:81035549-81035568 None:intergenic 20.0%
!! AAAAACAGGATTTGAGTTAA+AGG + chr7_3:81033320-81033339 Msa1129100:five_prime_UTR 25.0%
! GAAATGAGAAAATGAACTTA+CGG - chr7_3:81033543-81033562 None:intergenic 25.0%
! TCCAACTATATACTCTTTAA+AGG - chr7_3:81033652-81033671 None:intergenic 25.0%
!!! GATGAAGGTATTATTAAGTA+AGG - chr7_3:81034007-81034026 None:intergenic 25.0%
! ACAAGAAACATAAATATGGA+TGG - chr7_3:81034480-81034499 None:intergenic 25.0%
! GCTAACAAGAAACATAAATA+TGG - chr7_3:81034484-81034503 None:intergenic 25.0%
! GTTCAAAGAAGAGATATAAA+AGG + chr7_3:81034597-81034616 Msa1129100:CDS 25.0%
! AAAATGAAAAAGTTACTAGG+TGG - chr7_3:81034927-81034946 None:intergenic 25.0%
!! ATATTTTCAAAATCTGGGTT+TGG + chr7_3:81034995-81035014 Msa1129100:intron 25.0%
! TAAAGAAGACTCTAGTATAT+TGG - chr7_3:81035139-81035158 None:intergenic 25.0%
!! TACACTTGTACTATTTTATC+TGG + chr7_3:81035286-81035305 Msa1129100:CDS 25.0%
! GGTCATAATAATCAAATGTT+AGG + chr7_3:81035347-81035366 Msa1129100:three_prime_UTR 25.0%
!! TTGTTAATCTACAATTTTGG+GGG + chr7_3:81035401-81035420 Msa1129100:three_prime_UTR 25.0%
!! AAAAACAGGATTTGAGTTAA+AGG + chr7_3:81033320-81033339 Msa1129100:five_prime_UTR 25.0%
! GAAATGAGAAAATGAACTTA+CGG - chr7_3:81033543-81033562 None:intergenic 25.0%
! TCCAACTATATACTCTTTAA+AGG - chr7_3:81033652-81033671 None:intergenic 25.0%
!!! GATGAAGGTATTATTAAGTA+AGG - chr7_3:81034007-81034026 None:intergenic 25.0%
! ACAAGAAACATAAATATGGA+TGG - chr7_3:81034480-81034499 None:intergenic 25.0%
! GCTAACAAGAAACATAAATA+TGG - chr7_3:81034484-81034503 None:intergenic 25.0%
! GTTCAAAGAAGAGATATAAA+AGG + chr7_3:81034597-81034616 Msa1129100:CDS 25.0%
! AAAATGAAAAAGTTACTAGG+TGG - chr7_3:81034927-81034946 None:intergenic 25.0%
!! ATATTTTCAAAATCTGGGTT+TGG + chr7_3:81034995-81035014 Msa1129100:intron 25.0%
! TAAAGAAGACTCTAGTATAT+TGG - chr7_3:81035139-81035158 None:intergenic 25.0%
!! TACACTTGTACTATTTTATC+TGG + chr7_3:81035286-81035305 Msa1129100:CDS 25.0%
! GGTCATAATAATCAAATGTT+AGG + chr7_3:81035347-81035366 Msa1129100:three_prime_UTR 25.0%
!! TTGTTAATCTACAATTTTGG+GGG + chr7_3:81035401-81035420 Msa1129100:three_prime_UTR 25.0%
!!! AATCCTGTTTTTGTAAGACT+GGG - chr7_3:81033312-81033331 None:intergenic 30.0%
!!! AAATCCTGTTTTTGTAAGAC+TGG - chr7_3:81033313-81033332 None:intergenic 30.0%
!! GAGTTAAAGGTATCGAATAA+TGG + chr7_3:81033333-81033352 Msa1129100:exon 30.0%
TCTTGCTTTCTTCATTTCAT+CGG - chr7_3:81033619-81033638 None:intergenic 30.0%
ATGAAGAAAGCAAGAAAAGT+TGG + chr7_3:81033624-81033643 Msa1129100:CDS 30.0%
! GCCTTTAAAGAGTATATAGT+TGG + chr7_3:81033648-81033667 Msa1129100:CDS 30.0%
AACGAATCAAAAACATGAAG+CGG + chr7_3:81033839-81033858 Msa1129100:CDS 30.0%
!!! TCAGACATATTTTTAGGCAT+GGG - chr7_3:81033972-81033991 None:intergenic 30.0%
!!! TTCAGACATATTTTTAGGCA+TGG - chr7_3:81033973-81033992 None:intergenic 30.0%
!! GAAGGTATTATTAAGTAAGG+AGG - chr7_3:81034004-81034023 None:intergenic 30.0%
ATCACTCTCTAATTCTTCAA+GGG - chr7_3:81034061-81034080 None:intergenic 30.0%
AATCACTCTCTAATTCTTCA+AGG - chr7_3:81034062-81034081 None:intergenic 30.0%
TTAACACGAATCAACATCAT+CGG - chr7_3:81034158-81034177 None:intergenic 30.0%
!! ATGATGTTGATTCGTGTTAA+TGG + chr7_3:81034158-81034177 Msa1129100:intron 30.0%
!! GTTTTGTTGTCTTTTGTTGT+TGG + chr7_3:81034230-81034249 Msa1129100:intron 30.0%
TTTCGTTGTTTGCATGATTT+GGG + chr7_3:81034277-81034296 Msa1129100:intron 30.0%
ATGTGTTTCTTGTTCTTGTA+AGG + chr7_3:81034434-81034453 Msa1129100:intron 30.0%
TGTGTTTCTTGTTCTTGTAA+GGG + chr7_3:81034435-81034454 Msa1129100:intron 30.0%
!! ATTTATTTATGAAGGTGCTG+TGG + chr7_3:81035199-81035218 Msa1129100:intron 30.0%
AAAGGATTGAAAGAATTCAG+AGG + chr7_3:81035222-81035241 Msa1129100:CDS 30.0%
AACAAACTCAAGCAACTATT+TGG - chr7_3:81035386-81035405 None:intergenic 30.0%
!! ACAATTTTGGGGGTATATTT+AGG + chr7_3:81035411-81035430 Msa1129100:three_prime_UTR 30.0%
!!! AATCCTGTTTTTGTAAGACT+GGG - chr7_3:81033312-81033331 None:intergenic 30.0%
!!! AAATCCTGTTTTTGTAAGAC+TGG - chr7_3:81033313-81033332 None:intergenic 30.0%
!! GAGTTAAAGGTATCGAATAA+TGG + chr7_3:81033333-81033352 Msa1129100:exon 30.0%
TCTTGCTTTCTTCATTTCAT+CGG - chr7_3:81033619-81033638 None:intergenic 30.0%
ATGAAGAAAGCAAGAAAAGT+TGG + chr7_3:81033624-81033643 Msa1129100:CDS 30.0%
! GCCTTTAAAGAGTATATAGT+TGG + chr7_3:81033648-81033667 Msa1129100:CDS 30.0%
AACGAATCAAAAACATGAAG+CGG + chr7_3:81033839-81033858 Msa1129100:CDS 30.0%
!!! TCAGACATATTTTTAGGCAT+GGG - chr7_3:81033972-81033991 None:intergenic 30.0%
!!! TTCAGACATATTTTTAGGCA+TGG - chr7_3:81033973-81033992 None:intergenic 30.0%
!! GAAGGTATTATTAAGTAAGG+AGG - chr7_3:81034004-81034023 None:intergenic 30.0%
ATCACTCTCTAATTCTTCAA+GGG - chr7_3:81034061-81034080 None:intergenic 30.0%
AATCACTCTCTAATTCTTCA+AGG - chr7_3:81034062-81034081 None:intergenic 30.0%
TTAACACGAATCAACATCAT+CGG - chr7_3:81034158-81034177 None:intergenic 30.0%
!! ATGATGTTGATTCGTGTTAA+TGG + chr7_3:81034158-81034177 Msa1129100:intron 30.0%
!! GTTTTGTTGTCTTTTGTTGT+TGG + chr7_3:81034230-81034249 Msa1129100:intron 30.0%
TTTCGTTGTTTGCATGATTT+GGG + chr7_3:81034277-81034296 Msa1129100:intron 30.0%
ATGTGTTTCTTGTTCTTGTA+AGG + chr7_3:81034434-81034453 Msa1129100:intron 30.0%
TGTGTTTCTTGTTCTTGTAA+GGG + chr7_3:81034435-81034454 Msa1129100:intron 30.0%
!! ATTTATTTATGAAGGTGCTG+TGG + chr7_3:81035199-81035218 Msa1129100:intron 30.0%
AAAGGATTGAAAGAATTCAG+AGG + chr7_3:81035222-81035241 Msa1129100:CDS 30.0%
AACAAACTCAAGCAACTATT+TGG - chr7_3:81035386-81035405 None:intergenic 30.0%
!! ACAATTTTGGGGGTATATTT+AGG + chr7_3:81035411-81035430 Msa1129100:three_prime_UTR 30.0%
GAAAATGAACTTACGGAGTA+AGG - chr7_3:81033536-81033555 None:intergenic 35.0%
AATGCCTTCGTATTTCTCAA+TGG - chr7_3:81033703-81033722 None:intergenic 35.0%
ATGCACTTGCAAAGTTTACT+TGG + chr7_3:81033898-81033917 Msa1129100:CDS 35.0%
TGCACTTGCAAAGTTTACTT+GGG + chr7_3:81033899-81033918 Msa1129100:CDS 35.0%
CTTTCGTTGTTTGCATGATT+TGG + chr7_3:81034276-81034295 Msa1129100:intron 35.0%
TTCTTGTTAGCTCCAAAAGA+TGG + chr7_3:81034493-81034512 Msa1129100:intron 35.0%
CAAATCCAATGGATATTGCA+AGG + chr7_3:81034522-81034541 Msa1129100:CDS 35.0%
TTAGACCTTGCAATATCCAT+TGG - chr7_3:81034530-81034549 None:intergenic 35.0%
GGATATTGCAAGGTCTAATT+TGG + chr7_3:81034532-81034551 Msa1129100:CDS 35.0%
AGGTCTAATTTGGCTAAAAG+CGG + chr7_3:81034542-81034561 Msa1129100:CDS 35.0%
TATCTCTTCTTTGAACCACA+TGG - chr7_3:81034593-81034612 None:intergenic 35.0%
!! GTTATGTCCACTTGTTATCT+CGG + chr7_3:81034739-81034758 Msa1129100:CDS 35.0%
CTTTACATCGATCAGCAAAT+AGG + chr7_3:81034824-81034843 Msa1129100:intron 35.0%
AGGACAAGTATTAAACTGCA+AGG + chr7_3:81035242-81035261 Msa1129100:CDS 35.0%
TTCCCTTCAGCCTTAATAAA+AGG + chr7_3:81035490-81035509 Msa1129100:three_prime_UTR 35.0%
! TGCCTTTTATTAAGGCTGAA+GGG - chr7_3:81035495-81035514 None:intergenic 35.0%
! TTGCCTTTTATTAAGGCTGA+AGG - chr7_3:81035496-81035515 None:intergenic 35.0%
! TGGGTTCTTGCCTTTTATTA+AGG - chr7_3:81035503-81035522 None:intergenic 35.0%
GAAAATGAACTTACGGAGTA+AGG - chr7_3:81033536-81033555 None:intergenic 35.0%
AATGCCTTCGTATTTCTCAA+TGG - chr7_3:81033703-81033722 None:intergenic 35.0%
ATGCACTTGCAAAGTTTACT+TGG + chr7_3:81033898-81033917 Msa1129100:CDS 35.0%
TGCACTTGCAAAGTTTACTT+GGG + chr7_3:81033899-81033918 Msa1129100:CDS 35.0%
CTTTCGTTGTTTGCATGATT+TGG + chr7_3:81034276-81034295 Msa1129100:intron 35.0%
TTCTTGTTAGCTCCAAAAGA+TGG + chr7_3:81034493-81034512 Msa1129100:intron 35.0%
CAAATCCAATGGATATTGCA+AGG + chr7_3:81034522-81034541 Msa1129100:CDS 35.0%
TTAGACCTTGCAATATCCAT+TGG - chr7_3:81034530-81034549 None:intergenic 35.0%
GGATATTGCAAGGTCTAATT+TGG + chr7_3:81034532-81034551 Msa1129100:CDS 35.0%
AGGTCTAATTTGGCTAAAAG+CGG + chr7_3:81034542-81034561 Msa1129100:CDS 35.0%
TATCTCTTCTTTGAACCACA+TGG - chr7_3:81034593-81034612 None:intergenic 35.0%
!! GTTATGTCCACTTGTTATCT+CGG + chr7_3:81034739-81034758 Msa1129100:CDS 35.0%
CTTTACATCGATCAGCAAAT+AGG + chr7_3:81034824-81034843 Msa1129100:intron 35.0%
AGGACAAGTATTAAACTGCA+AGG + chr7_3:81035242-81035261 Msa1129100:CDS 35.0%
TTCCCTTCAGCCTTAATAAA+AGG + chr7_3:81035490-81035509 Msa1129100:three_prime_UTR 35.0%
! TGCCTTTTATTAAGGCTGAA+GGG - chr7_3:81035495-81035514 None:intergenic 35.0%
! TTGCCTTTTATTAAGGCTGA+AGG - chr7_3:81035496-81035515 None:intergenic 35.0%
! TGGGTTCTTGCCTTTTATTA+AGG - chr7_3:81035503-81035522 None:intergenic 35.0%
! GTTTTTGTAAGACTGGGAAG+TGG - chr7_3:81033306-81033325 None:intergenic 40.0%
CTTCCCAGTCTTACAAAAAC+AGG + chr7_3:81033306-81033325 Msa1129100:five_prime_UTR 40.0%
! GAACCGTAGTGGAAATTGAA+CGG - chr7_3:81033426-81033445 None:intergenic 40.0%
AAGGTCAAAAACGTCTTCAC+AGG + chr7_3:81033469-81033488 Msa1129100:CDS 40.0%
AAGGTGAATTGAGATGCATC+TGG - chr7_3:81033517-81033536 None:intergenic 40.0%
! GCAAACTCTGAGGACAAAAT+AGG - chr7_3:81033597-81033616 None:intergenic 40.0%
ACTGCCATTGAGAAATACGA+AGG + chr7_3:81033696-81033715 Msa1129100:CDS 40.0%
TAAGTGCACAATTGCTGAAG+TGG + chr7_3:81033872-81033891 Msa1129100:CDS 40.0%
TGTTAGCTCCAAAAGATGGT+CGG + chr7_3:81034497-81034516 Msa1129100:intron 40.0%
!! GATTTGTACCGACCATCTTT+TGG - chr7_3:81034508-81034527 None:intergenic 40.0%
!! TTTATGAAGGTGCTGTGGAA+AGG + chr7_3:81035204-81035223 Msa1129100:intron 40.0%
CAAGGAAGCCTTAACTGAAT+GGG + chr7_3:81035260-81035279 Msa1129100:CDS 40.0%
GTGTAGATCCCATTCAGTTA+AGG - chr7_3:81035271-81035290 None:intergenic 40.0%
GGCAAGAACCCAAATTGTTA+AGG + chr7_3:81035511-81035530 Msa1129100:three_prime_UTR 40.0%
ATTTGATCCCTAGCGTAATC+TGG + chr7_3:81035570-81035589 Msa1129100:three_prime_UTR 40.0%
TTCAAAGCCAGATTACGCTA+GGG - chr7_3:81035580-81035599 None:intergenic 40.0%
TTTCAAAGCCAGATTACGCT+AGG - chr7_3:81035581-81035600 None:intergenic 40.0%
! GTTTTTGTAAGACTGGGAAG+TGG - chr7_3:81033306-81033325 None:intergenic 40.0%
CTTCCCAGTCTTACAAAAAC+AGG + chr7_3:81033306-81033325 Msa1129100:five_prime_UTR 40.0%
! GAACCGTAGTGGAAATTGAA+CGG - chr7_3:81033426-81033445 None:intergenic 40.0%
AAGGTCAAAAACGTCTTCAC+AGG + chr7_3:81033469-81033488 Msa1129100:CDS 40.0%
AAGGTGAATTGAGATGCATC+TGG - chr7_3:81033517-81033536 None:intergenic 40.0%
! GCAAACTCTGAGGACAAAAT+AGG - chr7_3:81033597-81033616 None:intergenic 40.0%
ACTGCCATTGAGAAATACGA+AGG + chr7_3:81033696-81033715 Msa1129100:CDS 40.0%
TAAGTGCACAATTGCTGAAG+TGG + chr7_3:81033872-81033891 Msa1129100:CDS 40.0%
TGTTAGCTCCAAAAGATGGT+CGG + chr7_3:81034497-81034516 Msa1129100:intron 40.0%
!! GATTTGTACCGACCATCTTT+TGG - chr7_3:81034508-81034527 None:intergenic 40.0%
!! TTTATGAAGGTGCTGTGGAA+AGG + chr7_3:81035204-81035223 Msa1129100:intron 40.0%
CAAGGAAGCCTTAACTGAAT+GGG + chr7_3:81035260-81035279 Msa1129100:CDS 40.0%
GTGTAGATCCCATTCAGTTA+AGG - chr7_3:81035271-81035290 None:intergenic 40.0%
GGCAAGAACCCAAATTGTTA+AGG + chr7_3:81035511-81035530 Msa1129100:three_prime_UTR 40.0%
ATTTGATCCCTAGCGTAATC+TGG + chr7_3:81035570-81035589 Msa1129100:three_prime_UTR 40.0%
TTCAAAGCCAGATTACGCTA+GGG - chr7_3:81035580-81035599 None:intergenic 40.0%
TTTCAAAGCCAGATTACGCT+AGG - chr7_3:81035581-81035600 None:intergenic 40.0%
AAGACTGGGAAGTGGAAAAC+TGG - chr7_3:81033298-81033317 None:intergenic 45.0%
TAGTGGAAATTGAACGGTGG+TGG - chr7_3:81033420-81033439 None:intergenic 45.0%
CCACCGTTCAATTTCCACTA+CGG + chr7_3:81033420-81033439 Msa1129100:CDS 45.0%
TCACCTTCAGAGTTCATGGA+TGG + chr7_3:81033444-81033463 Msa1129100:CDS 45.0%
TGTCCATCCATGAACTCTGA+AGG - chr7_3:81033450-81033469 None:intergenic 45.0%
TCAGAGTTCATGGATGGACA+AGG + chr7_3:81033450-81033469 Msa1129100:CDS 45.0%
TTCACAGGCCAAAAATCCGA+AGG + chr7_3:81033484-81033503 Msa1129100:CDS 45.0%
GTGAATTGAGATGCATCTGG+TGG - chr7_3:81033514-81033533 None:intergenic 45.0%
CATTTCATCGGCAAACTCTG+AGG - chr7_3:81033607-81033626 None:intergenic 45.0%
AATCGAATGCGGCGAGATAT+CGG - chr7_3:81033728-81033747 None:intergenic 45.0%
CTCTCCTGTGTAATCGAATG+CGG - chr7_3:81033739-81033758 None:intergenic 45.0%
GATGGTCGGTACAAATCCAA+TGG + chr7_3:81034511-81034530 Msa1129100:CDS 45.0%
CTAAAAGCGGACAAGTTAGC+AGG + chr7_3:81034555-81034574 Msa1129100:CDS 45.0%
CTTTGAACCACATGGACAGA+GGG - chr7_3:81034585-81034604 None:intergenic 45.0%
TCTTTGAACCACATGGACAG+AGG - chr7_3:81034586-81034605 None:intergenic 45.0%
TAGCAAGCCGAGATAACAAG+TGG - chr7_3:81034749-81034768 None:intergenic 45.0%
!! TCTCGGCTTGCTAAAGTTCT+TGG + chr7_3:81034756-81034775 Msa1129100:CDS 45.0%
!! CTCGGCTTGCTAAAGTTCTT+GGG + chr7_3:81034757-81034776 Msa1129100:CDS 45.0%
!! TCGGCTTGCTAAAGTTCTTG+GGG + chr7_3:81034758-81034777 Msa1129100:CDS 45.0%
GCAAGGAAGCCTTAACTGAA+TGG + chr7_3:81035259-81035278 Msa1129100:CDS 45.0%
AATGCACTAGACTGCAGACA+TGG + chr7_3:81035326-81035345 Msa1129100:exon 45.0%
AAGACTGGGAAGTGGAAAAC+TGG - chr7_3:81033298-81033317 None:intergenic 45.0%
TAGTGGAAATTGAACGGTGG+TGG - chr7_3:81033420-81033439 None:intergenic 45.0%
CCACCGTTCAATTTCCACTA+CGG + chr7_3:81033420-81033439 Msa1129100:CDS 45.0%
TCACCTTCAGAGTTCATGGA+TGG + chr7_3:81033444-81033463 Msa1129100:CDS 45.0%
TGTCCATCCATGAACTCTGA+AGG - chr7_3:81033450-81033469 None:intergenic 45.0%
TCAGAGTTCATGGATGGACA+AGG + chr7_3:81033450-81033469 Msa1129100:CDS 45.0%
TTCACAGGCCAAAAATCCGA+AGG + chr7_3:81033484-81033503 Msa1129100:CDS 45.0%
GTGAATTGAGATGCATCTGG+TGG - chr7_3:81033514-81033533 None:intergenic 45.0%
CATTTCATCGGCAAACTCTG+AGG - chr7_3:81033607-81033626 None:intergenic 45.0%
AATCGAATGCGGCGAGATAT+CGG - chr7_3:81033728-81033747 None:intergenic 45.0%
CTCTCCTGTGTAATCGAATG+CGG - chr7_3:81033739-81033758 None:intergenic 45.0%
GATGGTCGGTACAAATCCAA+TGG + chr7_3:81034511-81034530 Msa1129100:CDS 45.0%
CTAAAAGCGGACAAGTTAGC+AGG + chr7_3:81034555-81034574 Msa1129100:CDS 45.0%
CTTTGAACCACATGGACAGA+GGG - chr7_3:81034585-81034604 None:intergenic 45.0%
TCTTTGAACCACATGGACAG+AGG - chr7_3:81034586-81034605 None:intergenic 45.0%
TAGCAAGCCGAGATAACAAG+TGG - chr7_3:81034749-81034768 None:intergenic 45.0%
!! TCTCGGCTTGCTAAAGTTCT+TGG + chr7_3:81034756-81034775 Msa1129100:CDS 45.0%
!! CTCGGCTTGCTAAAGTTCTT+GGG + chr7_3:81034757-81034776 Msa1129100:CDS 45.0%
!! TCGGCTTGCTAAAGTTCTTG+GGG + chr7_3:81034758-81034777 Msa1129100:CDS 45.0%
GCAAGGAAGCCTTAACTGAA+TGG + chr7_3:81035259-81035278 Msa1129100:CDS 45.0%
AATGCACTAGACTGCAGACA+TGG + chr7_3:81035326-81035345 Msa1129100:exon 45.0%
!!! TGTATTATTTATTTTTATAT+AGG - chr7_3:81034190-81034209 None:intergenic 5.0%
!!! TGTATTATTTATTTTTATAT+AGG - chr7_3:81034190-81034209 None:intergenic 5.0%
TCTGTTTGCGATGCGAAGTG+CGG - chr7_3:81033359-81033378 None:intergenic 50.0%
! CCGTAGTGGAAATTGAACGG+TGG - chr7_3:81033423-81033442 None:intergenic 50.0%
! ACTCTGAAGGTGAACCGTAG+TGG - chr7_3:81033437-81033456 None:intergenic 50.0%
CGGTTCACCTTCAGAGTTCA+TGG + chr7_3:81033440-81033459 Msa1129100:CDS 50.0%
! GTGGTGCTCCTTCGGATTTT+TGG - chr7_3:81033495-81033514 None:intergenic 50.0%
CGCATTCGATTACACAGGAG+AGG + chr7_3:81033737-81033756 Msa1129100:CDS 50.0%
CTGCAGTCTAGTGCATTGAG+CGG - chr7_3:81035323-81035342 None:intergenic 50.0%
TCTGTTTGCGATGCGAAGTG+CGG - chr7_3:81033359-81033378 None:intergenic 50.0%
! CCGTAGTGGAAATTGAACGG+TGG - chr7_3:81033423-81033442 None:intergenic 50.0%
! ACTCTGAAGGTGAACCGTAG+TGG - chr7_3:81033437-81033456 None:intergenic 50.0%
CGGTTCACCTTCAGAGTTCA+TGG + chr7_3:81033440-81033459 Msa1129100:CDS 50.0%
! GTGGTGCTCCTTCGGATTTT+TGG - chr7_3:81033495-81033514 None:intergenic 50.0%
CGCATTCGATTACACAGGAG+AGG + chr7_3:81033737-81033756 Msa1129100:CDS 50.0%
CTGCAGTCTAGTGCATTGAG+CGG - chr7_3:81035323-81035342 None:intergenic 50.0%
! TGCATCTGGTGGTGCTCCTT+CGG - chr7_3:81033503-81033522 None:intergenic 55.0%
CTCGCCGCATTCGATTACAC+AGG + chr7_3:81033732-81033751 Msa1129100:CDS 55.0%
AGGAATGCCCTCTGTCCATG+TGG + chr7_3:81034575-81034594 Msa1129100:CDS 55.0%
! TGCATCTGGTGGTGCTCCTT+CGG - chr7_3:81033503-81033522 None:intergenic 55.0%
CTCGCCGCATTCGATTACAC+AGG + chr7_3:81033732-81033751 Msa1129100:CDS 55.0%
AGGAATGCCCTCTGTCCATG+TGG + chr7_3:81034575-81034594 Msa1129100:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 81033277 81035621 81033277 ID=Msa1129100;Name=Msa1129100
chr7_3 mRNA 81033277 81035621 81033277 ID=Msa1129100-mRNA-1;Parent=Msa1129100;Name=Msa1129100-mRNA-1;_AED=0.08;_eAED=0.08;_QI=75|0.6|0.83|0.83|0.8|0.83|6|286|263
chr7_3 exon 81033277 81033541 81033277 ID=Msa1129100-mRNA-1:exon:23438;Parent=Msa1129100-mRNA-1
chr7_3 exon 81033610 81033758 81033610 ID=Msa1129100-mRNA-1:exon:23439;Parent=Msa1129100-mRNA-1
chr7_3 exon 81033831 81033992 81033831 ID=Msa1129100-mRNA-1:exon:23440;Parent=Msa1129100-mRNA-1
chr7_3 exon 81034503 81034618 81034503 ID=Msa1129100-mRNA-1:exon:23441;Parent=Msa1129100-mRNA-1
chr7_3 exon 81034728 81034779 81034728 ID=Msa1129100-mRNA-1:exon:23442;Parent=Msa1129100-mRNA-1
chr7_3 exon 81035213 81035621 81035213 ID=Msa1129100-mRNA-1:exon:23443;Parent=Msa1129100-mRNA-1
chr7_3 five_prime_UTR 81033277 81033351 81033277 ID=Msa1129100-mRNA-1:five_prime_utr;Parent=Msa1129100-mRNA-1
chr7_3 CDS 81033352 81033541 81033352 ID=Msa1129100-mRNA-1:cds;Parent=Msa1129100-mRNA-1
chr7_3 CDS 81033610 81033758 81033610 ID=Msa1129100-mRNA-1:cds;Parent=Msa1129100-mRNA-1
chr7_3 CDS 81033831 81033992 81033831 ID=Msa1129100-mRNA-1:cds;Parent=Msa1129100-mRNA-1
chr7_3 CDS 81034503 81034618 81034503 ID=Msa1129100-mRNA-1:cds;Parent=Msa1129100-mRNA-1
chr7_3 CDS 81034728 81034779 81034728 ID=Msa1129100-mRNA-1:cds;Parent=Msa1129100-mRNA-1
chr7_3 CDS 81035213 81035335 81035213 ID=Msa1129100-mRNA-1:cds;Parent=Msa1129100-mRNA-1
chr7_3 three_prime_UTR 81035336 81035621 81035336 ID=Msa1129100-mRNA-1:three_prime_utr;Parent=Msa1129100-mRNA-1
Gene Sequence

>Msa1129100

ATGGCCGCACTTCGCATCGCAAACAGAATCATAAATTATCATCATAATCTCTTATACAACAATTACCACCACCGTTCAATTTCCACTACGGTTCACCTTCAGAGTTCATGGATGGACAAGGTCAAAAACGTCTTCACAGGCCAAAAATCCGAAGGAGCACCACCAGATGCATCTCAATTCACCTTACTCCAGTTTGCCGATGAAATGAAGAAAGCAAGAAAAGTTGGTGCCTTTAAAGAGTATATAGTTGGACGAAGCAGCGAAGTTACTTTTAGTACTGCCATTGAGAAATACGAAGGCATTATCCGATATCTCGCCGCATTCGATTACACAGGAGAGAACTTGACAACGAATCAAAAACATGAAGCGGCGAAACATTGTAAGTGCACAATTGCTGAAGTGGAGAATGCACTTGCAAAGTTTACTTGGGCTAAAGAAGCACACAAGAAGATTCAAAAGTTAAATCAAGAAGGAAAACCCATGCCTAAAAATATGTCTGAACTCCAAAAGATGGTCGGTACAAATCCAATGGATATTGCAAGGTCTAATTTGGCTAAAAGCGGACAAGTTAGCAGGAATGCCCTCTGTCCATGTGGTTCAAAGAAGAGATATAAAAGAAAAGCAAAATGTTATGTCCACTTGTTATCTCGGCTTGCTAAAGTTCTTGGGGTGCTGTGGAAAGGATTGAAAGAATTCAGAGGACAAGTATTAAACTGCAAGGAAGCCTTAACTGAATGGGATCTACACTTGTACTATTTTATCTGGTTCAGATCAAGCCGCTCAATGCACTAG
Protein sequence

>Msa1129100

MAALRIANRIINYHHNLLYNNYHHRSISTTVHLQSSWMDKVKNVFTGQKSEGAPPDASQFTLLQFADEMKKARKVGAFKEYIVGRSSEVTFSTAIEKYEGIIRYLAAFDYTGENLTTNQKHEAAKHCKCTIAEVENALAKFTWAKEAHKKIQKLNQEGKPMPKNMSELQKMVGTNPMDIARSNLAKSGQVSRNALCPCGSKKRYKRKAKCYVHLLSRLAKVLGVLWKGLKEFRGQVLNCKEALTEWDLHLYYFIWFRSSRSMH*