AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1388680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388680 MtrunA17_Chr7g0273641 96.602 206 7 0 1 206 1 206 3.26e-150 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388680 AT3G04950.1 64.706 187 62 2 23 206 41 226 1.84e-86 257

Find 68 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTGTTAATCTACAATTT+TGG 0.108080 tig0021157:+63943 Msa1388680:three_prime_UTR
TAAATTATGCCTTAACAATT+TGG 0.113030 tig0021157:-64065 None:intergenic
TCCAACTATATACTCTTTAA+AGG 0.178788 tig0021157:-62194 None:intergenic
GGATATTGCAAGGTCTAATT+TGG 0.200253 tig0021157:+63077 Msa1388680:CDS
AACAAACTCAAGCAACTATT+TGG 0.211577 tig0021157:-63928 None:intergenic
TTCCCTTCAGCCTTAATAAA+AGG 0.215422 tig0021157:+64035 Msa1388680:three_prime_UTR
TCTCGGCTTGCTAAAGTTCT+TGG 0.252133 tig0021157:+63301 Msa1388680:CDS
CTCGGCTTGCTAAAGTTCTT+GGG 0.291086 tig0021157:+63302 Msa1388680:CDS
GATTTGTACCGACCATCTTT+TGG 0.311365 tig0021157:-63050 None:intergenic
ATTTGATCCCTAGCGTAATC+TGG 0.335969 tig0021157:+64115 Msa1388680:three_prime_UTR
CGGTTCACCTTCAGAGTTCA+TGG 0.336324 tig0021157:+61985 Msa1388680:CDS
AAATTATGCCTTAACAATTT+GGG 0.336813 tig0021157:-64064 None:intergenic
ATGCACTTGCAAAGTTTACT+TGG 0.358285 tig0021157:+62443 Msa1388680:CDS
GGTCATAATAATCAAATGTT+AGG 0.373371 tig0021157:+63892 Msa1388680:three_prime_UTR
TGCATCTGGTGGTGCTCCTT+CGG 0.382519 tig0021157:-62045 None:intergenic
GTTCAAAGAAGAGATATAAA+AGG 0.388106 tig0021157:+63142 Msa1388680:CDS
GAGTTAAAGGTATCGAATAA+TGG 0.404497 tig0021157:+61878 Msa1388680:exon
GAAAATGAACTTACGGAGTA+AGG 0.422469 tig0021157:-62078 None:intergenic
AAGACTGGGAAGTGGAAAAC+TGG 0.423192 tig0021157:-61840 None:intergenic
GGGGAAAAGGCAATTTGGGT+TGG 0.426545 tig0021157:+61814 None:intergenic
GTTATGTCCACTTGTTATCT+CGG 0.430472 tig0021157:+63284 Msa1388680:CDS
GCCTTTAAAGAGTATATAGT+TGG 0.433265 tig0021157:+62193 Msa1388680:CDS
AATCGAATGCGGCGAGATAT+CGG 0.442505 tig0021157:-62270 None:intergenic
GTGTAGATCCCATTCAGTTA+AGG 0.446462 tig0021157:-63813 None:intergenic
GGCAAGAACCCAAATTGTTA+AGG 0.454046 tig0021157:+64056 Msa1388680:three_prime_UTR
TCTTGCTTTCTTCATTTCAT+CGG 0.458556 tig0021157:-62161 None:intergenic
ATGAAGAAAGCAAGAAAAGT+TGG 0.472505 tig0021157:+62169 Msa1388680:CDS
TTAGACCTTGCAATATCCAT+TGG 0.473307 tig0021157:-63072 None:intergenic
GCAAGGAAGCCTTAACTGAA+TGG 0.480468 tig0021157:+63804 Msa1388680:CDS
TTTCAAAGCCAGATTACGCT+AGG 0.491492 tig0021157:-64123 None:intergenic
TTCTTGTTAGCTCCAAAAGA+TGG 0.497318 tig0021157:+63038 Msa1388680:intron
CAAGGAAGCCTTAACTGAAT+GGG 0.501539 tig0021157:+63805 Msa1388680:CDS
CTAAAAGCGGACAAGTTAGC+AGG 0.515399 tig0021157:+63100 Msa1388680:CDS
AATGCCTTCGTATTTCTCAA+TGG 0.521342 tig0021157:-62245 None:intergenic
TGCACTTGCAAAGTTTACTT+GGG 0.524528 tig0021157:+62444 Msa1388680:CDS
TTTATGAAGGTGCTGTGGAA+AGG 0.546367 tig0021157:+63749 Msa1388680:intron
TAAGTGCACAATTGCTGAAG+TGG 0.546585 tig0021157:+62417 Msa1388680:CDS
CCACCGTTCAATTTCCACTA+CGG 0.551541 tig0021157:+61965 Msa1388680:CDS
ATTCAAAAGTTAAATCAAGA+AGG 0.556894 tig0021157:+62487 Msa1388680:CDS
TCGGCTTGCTAAAGTTCTTG+GGG 0.563252 tig0021157:+63303 Msa1388680:CDS
TATCTCTTCTTTGAACCACA+TGG 0.570464 tig0021157:-63135 None:intergenic
GATGGTCGGTACAAATCCAA+TGG 0.571490 tig0021157:+63056 Msa1388680:CDS
TCACCTTCAGAGTTCATGGA+TGG 0.571753 tig0021157:+61989 Msa1388680:CDS
TTCAAAGCCAGATTACGCTA+GGG 0.572480 tig0021157:-64122 None:intergenic
CTTTGAACCACATGGACAGA+GGG 0.580056 tig0021157:-63127 None:intergenic
ATTTATTTATGAAGGTGCTG+TGG 0.584119 tig0021157:+63744 Msa1388680:intron
TAGTGGAAATTGAACGGTGG+TGG 0.584928 tig0021157:-61962 None:intergenic
CAAATCCAATGGATATTGCA+AGG 0.593492 tig0021157:+63067 Msa1388680:CDS
TGTCCATCCATGAACTCTGA+AGG 0.596448 tig0021157:-61992 None:intergenic
CGCATTCGATTACACAGGAG+AGG 0.602676 tig0021157:+62282 Msa1388680:CDS
AAGGTGAATTGAGATGCATC+TGG 0.611379 tig0021157:-62059 None:intergenic
ACTCTGAAGGTGAACCGTAG+TGG 0.617132 tig0021157:-61979 None:intergenic
AATGCACTAGACTGCAGACA+TGG 0.621775 tig0021157:+63871 Msa1388680:exon
AGGAATGCCCTCTGTCCATG+TGG 0.628191 tig0021157:+63120 Msa1388680:CDS
AGGTCTAATTTGGCTAAAAG+CGG 0.628257 tig0021157:+63087 Msa1388680:CDS
AGGACAAGTATTAAACTGCA+AGG 0.629058 tig0021157:+63787 Msa1388680:CDS
TGTTAGCTCCAAAAGATGGT+CGG 0.634247 tig0021157:+63042 Msa1388680:intron
TAGCAAGCCGAGATAACAAG+TGG 0.636899 tig0021157:-63291 None:intergenic
TCTTTGAACCACATGGACAG+AGG 0.637245 tig0021157:-63128 None:intergenic
CTCTCCTGTGTAATCGAATG+CGG 0.644799 tig0021157:-62281 None:intergenic
GAACCGTAGTGGAAATTGAA+CGG 0.645642 tig0021157:-61968 None:intergenic
AAAGGATTGAAAGAATTCAG+AGG 0.649542 tig0021157:+63767 Msa1388680:CDS
TCAGAGTTCATGGATGGACA+AGG 0.658450 tig0021157:+61995 Msa1388680:CDS
TCTGTTTGCGATGCGAAGTG+CGG 0.662046 tig0021157:-61901 None:intergenic
CTGCAGTCTAGTGCATTGAG+CGG 0.670629 tig0021157:-63865 None:intergenic
ACTGCCATTGAGAAATACGA+AGG 0.691670 tig0021157:+62241 Msa1388680:CDS
GTGAATTGAGATGCATCTGG+TGG 0.748795 tig0021157:-62056 None:intergenic
CCGTAGTGGAAATTGAACGG+TGG 0.810192 tig0021157:-61965 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTAATAAAATGATGA+AGG - tig0021157:62567-62586 None:intergenic 10.0%
!! TAAAATTAATAAAATGATGA+AGG - tig0021157:62567-62586 None:intergenic 10.0%
!! TAAAAAATGAAAAAGTTACT+AGG - tig0021157:63475-63494 None:intergenic 15.0%
!!! TTTTGTATATTTTCAAAATC+TGG + tig0021157:63534-63553 Msa1388680:intron 15.0%
!!! TTTGTATATTTTCAAAATCT+GGG + tig0021157:63535-63554 Msa1388680:intron 15.0%
!! AATTCAAATTCAAAACAATT+GGG - tig0021157:63655-63674 None:intergenic 15.0%
!! AAATTCAAATTCAAAACAAT+TGG - tig0021157:63656-63675 None:intergenic 15.0%
!! TTATGAGTATTTATTTATGA+AGG + tig0021157:63736-63755 Msa1388680:intron 15.0%
!! ATTAACATATTTCACAATTT+CGG + tig0021157:64146-64165 Msa1388680:three_prime_UTR 15.0%
!! TAAAAAATGAAAAAGTTACT+AGG - tig0021157:63475-63494 None:intergenic 15.0%
!!! TTTTGTATATTTTCAAAATC+TGG + tig0021157:63534-63553 Msa1388680:intron 15.0%
!!! TTTGTATATTTTCAAAATCT+GGG + tig0021157:63535-63554 Msa1388680:intron 15.0%
!! AATTCAAATTCAAAACAATT+GGG - tig0021157:63655-63674 None:intergenic 15.0%
!! AAATTCAAATTCAAAACAAT+TGG - tig0021157:63656-63675 None:intergenic 15.0%
!! TTATGAGTATTTATTTATGA+AGG + tig0021157:63736-63755 Msa1388680:intron 15.0%
!!! TTGTTGATTTTGTTTTGTTA+TGG + tig0021157:62349-62368 Msa1388680:intron 20.0%
!! ATTCAAAAGTTAAATCAAGA+AGG + tig0021157:62487-62506 Msa1388680:CDS 20.0%
!!! ATTACTTCAGACATATTTTT+AGG - tig0021157:62523-62542 None:intergenic 20.0%
!!! GAAGATTTTCTTTTTTTGAA+CGG - tig0021157:63182-63201 None:intergenic 20.0%
!!! ATAGAAAAGTACTAAGTTAT+AGG - tig0021157:63422-63441 None:intergenic 20.0%
!! ACTAATCTAATATCTATCAA+AGG - tig0021157:63446-63465 None:intergenic 20.0%
!! ATTCAAATTCAAAACAATTG+GGG - tig0021157:63654-63673 None:intergenic 20.0%
!!! AGTTTGTTAATCTACAATTT+TGG + tig0021157:63943-63962 Msa1388680:three_prime_UTR 20.0%
!!! GTTTGTTAATCTACAATTTT+GGG + tig0021157:63944-63963 Msa1388680:three_prime_UTR 20.0%
!!! TTTGTTAATCTACAATTTTG+GGG + tig0021157:63945-63964 Msa1388680:three_prime_UTR 20.0%
!! AAATTATGCCTTAACAATTT+GGG - tig0021157:64067-64086 None:intergenic 20.0%
!! TAAATTATGCCTTAACAATT+TGG - tig0021157:64068-64087 None:intergenic 20.0%
!! ATTAAAAAAGTGAGATTACA+GGG - tig0021157:64093-64112 None:intergenic 20.0%
!! AATTAAAAAAGTGAGATTAC+AGG - tig0021157:64094-64113 None:intergenic 20.0%
!!! TTGTTGATTTTGTTTTGTTA+TGG + tig0021157:62349-62368 Msa1388680:intron 20.0%
!! ATTCAAAAGTTAAATCAAGA+AGG + tig0021157:62487-62506 Msa1388680:CDS 20.0%
!!! ATTACTTCAGACATATTTTT+AGG - tig0021157:62523-62542 None:intergenic 20.0%
!!! GAAGATTTTCTTTTTTTGAA+CGG - tig0021157:63182-63201 None:intergenic 20.0%
!!! ATAGAAAAGTACTAAGTTAT+AGG - tig0021157:63422-63441 None:intergenic 20.0%
!! ACTAATCTAATATCTATCAA+AGG - tig0021157:63446-63465 None:intergenic 20.0%
!! ATTCAAATTCAAAACAATTG+GGG - tig0021157:63654-63673 None:intergenic 20.0%
!!! AGTTTGTTAATCTACAATTT+TGG + tig0021157:63943-63962 Msa1388680:three_prime_UTR 20.0%
!!! GTTTGTTAATCTACAATTTT+GGG + tig0021157:63944-63963 Msa1388680:three_prime_UTR 20.0%
!!! TTTGTTAATCTACAATTTTG+GGG + tig0021157:63945-63964 Msa1388680:three_prime_UTR 20.0%
!! AAATTATGCCTTAACAATTT+GGG - tig0021157:64067-64086 None:intergenic 20.0%
!! TAAATTATGCCTTAACAATT+TGG - tig0021157:64068-64087 None:intergenic 20.0%
!! ATTAAAAAAGTGAGATTACA+GGG - tig0021157:64093-64112 None:intergenic 20.0%
!! AATTAAAAAAGTGAGATTAC+AGG - tig0021157:64094-64113 None:intergenic 20.0%
!! AAAAACAGGATTTGAGTTAA+AGG + tig0021157:61865-61884 Msa1388680:five_prime_UTR 25.0%
! GAAATGAGAAAATGAACTTA+CGG - tig0021157:62088-62107 None:intergenic 25.0%
! TCCAACTATATACTCTTTAA+AGG - tig0021157:62197-62216 None:intergenic 25.0%
!!! GATGAAGGTATTATTAAGTA+AGG - tig0021157:62552-62571 None:intergenic 25.0%
! ACAAGAAACATAAATATGGA+TGG - tig0021157:63025-63044 None:intergenic 25.0%
! GCTAACAAGAAACATAAATA+TGG - tig0021157:63029-63048 None:intergenic 25.0%
! GTTCAAAGAAGAGATATAAA+AGG + tig0021157:63142-63161 Msa1388680:CDS 25.0%
! AAAATGAAAAAGTTACTAGG+TGG - tig0021157:63472-63491 None:intergenic 25.0%
!! ATATTTTCAAAATCTGGGTT+TGG + tig0021157:63540-63559 Msa1388680:intron 25.0%
! TAAAGAAGACTCTAGTATAT+TGG - tig0021157:63684-63703 None:intergenic 25.0%
!! TACACTTGTACTATTTTATC+TGG + tig0021157:63831-63850 Msa1388680:CDS 25.0%
! GGTCATAATAATCAAATGTT+AGG + tig0021157:63892-63911 Msa1388680:three_prime_UTR 25.0%
!! TTGTTAATCTACAATTTTGG+GGG + tig0021157:63946-63965 Msa1388680:three_prime_UTR 25.0%
!! AAAAACAGGATTTGAGTTAA+AGG + tig0021157:61865-61884 Msa1388680:five_prime_UTR 25.0%
! GAAATGAGAAAATGAACTTA+CGG - tig0021157:62088-62107 None:intergenic 25.0%
! TCCAACTATATACTCTTTAA+AGG - tig0021157:62197-62216 None:intergenic 25.0%
!!! GATGAAGGTATTATTAAGTA+AGG - tig0021157:62552-62571 None:intergenic 25.0%
! ACAAGAAACATAAATATGGA+TGG - tig0021157:63025-63044 None:intergenic 25.0%
! GCTAACAAGAAACATAAATA+TGG - tig0021157:63029-63048 None:intergenic 25.0%
! GTTCAAAGAAGAGATATAAA+AGG + tig0021157:63142-63161 Msa1388680:CDS 25.0%
! AAAATGAAAAAGTTACTAGG+TGG - tig0021157:63472-63491 None:intergenic 25.0%
!! ATATTTTCAAAATCTGGGTT+TGG + tig0021157:63540-63559 Msa1388680:intron 25.0%
! TAAAGAAGACTCTAGTATAT+TGG - tig0021157:63684-63703 None:intergenic 25.0%
!! TACACTTGTACTATTTTATC+TGG + tig0021157:63831-63850 Msa1388680:CDS 25.0%
! GGTCATAATAATCAAATGTT+AGG + tig0021157:63892-63911 Msa1388680:three_prime_UTR 25.0%
!! TTGTTAATCTACAATTTTGG+GGG + tig0021157:63946-63965 Msa1388680:three_prime_UTR 25.0%
!!! AATCCTGTTTTTGTAAGACT+GGG - tig0021157:61857-61876 None:intergenic 30.0%
!!! AAATCCTGTTTTTGTAAGAC+TGG - tig0021157:61858-61877 None:intergenic 30.0%
!! GAGTTAAAGGTATCGAATAA+TGG + tig0021157:61878-61897 Msa1388680:exon 30.0%
TCTTGCTTTCTTCATTTCAT+CGG - tig0021157:62164-62183 None:intergenic 30.0%
ATGAAGAAAGCAAGAAAAGT+TGG + tig0021157:62169-62188 Msa1388680:CDS 30.0%
! GCCTTTAAAGAGTATATAGT+TGG + tig0021157:62193-62212 Msa1388680:CDS 30.0%
AACGAATCAAAAACATGAAG+CGG + tig0021157:62384-62403 Msa1388680:CDS 30.0%
!!! TCAGACATATTTTTAGGCAT+GGG - tig0021157:62517-62536 None:intergenic 30.0%
!!! TTCAGACATATTTTTAGGCA+TGG - tig0021157:62518-62537 None:intergenic 30.0%
!! GAAGGTATTATTAAGTAAGG+AGG - tig0021157:62549-62568 None:intergenic 30.0%
ATCACTCTCTAATTCTTCAA+GGG - tig0021157:62606-62625 None:intergenic 30.0%
AATCACTCTCTAATTCTTCA+AGG - tig0021157:62607-62626 None:intergenic 30.0%
TTAACACGAATCAACATCAT+CGG - tig0021157:62703-62722 None:intergenic 30.0%
!! ATGATGTTGATTCGTGTTAA+TGG + tig0021157:62703-62722 Msa1388680:intron 30.0%
!! GTTTTGTTGTCTTTTGTTGT+TGG + tig0021157:62775-62794 Msa1388680:intron 30.0%
TTTCGTTGTTTGCATGATTT+GGG + tig0021157:62822-62841 Msa1388680:intron 30.0%
ATGTGTTTCTTGTTCTTGTA+AGG + tig0021157:62979-62998 Msa1388680:intron 30.0%
TGTGTTTCTTGTTCTTGTAA+GGG + tig0021157:62980-62999 Msa1388680:intron 30.0%
!! ATTTATTTATGAAGGTGCTG+TGG + tig0021157:63744-63763 Msa1388680:intron 30.0%
AAAGGATTGAAAGAATTCAG+AGG + tig0021157:63767-63786 Msa1388680:CDS 30.0%
AACAAACTCAAGCAACTATT+TGG - tig0021157:63931-63950 None:intergenic 30.0%
!! ACAATTTTGGGGGTATATTT+AGG + tig0021157:63956-63975 Msa1388680:three_prime_UTR 30.0%
!!! AATCCTGTTTTTGTAAGACT+GGG - tig0021157:61857-61876 None:intergenic 30.0%
!!! AAATCCTGTTTTTGTAAGAC+TGG - tig0021157:61858-61877 None:intergenic 30.0%
!! GAGTTAAAGGTATCGAATAA+TGG + tig0021157:61878-61897 Msa1388680:exon 30.0%
TCTTGCTTTCTTCATTTCAT+CGG - tig0021157:62164-62183 None:intergenic 30.0%
ATGAAGAAAGCAAGAAAAGT+TGG + tig0021157:62169-62188 Msa1388680:CDS 30.0%
! GCCTTTAAAGAGTATATAGT+TGG + tig0021157:62193-62212 Msa1388680:CDS 30.0%
AACGAATCAAAAACATGAAG+CGG + tig0021157:62384-62403 Msa1388680:CDS 30.0%
!!! TCAGACATATTTTTAGGCAT+GGG - tig0021157:62517-62536 None:intergenic 30.0%
!!! TTCAGACATATTTTTAGGCA+TGG - tig0021157:62518-62537 None:intergenic 30.0%
!! GAAGGTATTATTAAGTAAGG+AGG - tig0021157:62549-62568 None:intergenic 30.0%
ATCACTCTCTAATTCTTCAA+GGG - tig0021157:62606-62625 None:intergenic 30.0%
AATCACTCTCTAATTCTTCA+AGG - tig0021157:62607-62626 None:intergenic 30.0%
TTAACACGAATCAACATCAT+CGG - tig0021157:62703-62722 None:intergenic 30.0%
!! ATGATGTTGATTCGTGTTAA+TGG + tig0021157:62703-62722 Msa1388680:intron 30.0%
!! GTTTTGTTGTCTTTTGTTGT+TGG + tig0021157:62775-62794 Msa1388680:intron 30.0%
TTTCGTTGTTTGCATGATTT+GGG + tig0021157:62822-62841 Msa1388680:intron 30.0%
ATGTGTTTCTTGTTCTTGTA+AGG + tig0021157:62979-62998 Msa1388680:intron 30.0%
TGTGTTTCTTGTTCTTGTAA+GGG + tig0021157:62980-62999 Msa1388680:intron 30.0%
!! ATTTATTTATGAAGGTGCTG+TGG + tig0021157:63744-63763 Msa1388680:intron 30.0%
AAAGGATTGAAAGAATTCAG+AGG + tig0021157:63767-63786 Msa1388680:CDS 30.0%
AACAAACTCAAGCAACTATT+TGG - tig0021157:63931-63950 None:intergenic 30.0%
!! ACAATTTTGGGGGTATATTT+AGG + tig0021157:63956-63975 Msa1388680:three_prime_UTR 30.0%
GAAAATGAACTTACGGAGTA+AGG - tig0021157:62081-62100 None:intergenic 35.0%
AATGCCTTCGTATTTCTCAA+TGG - tig0021157:62248-62267 None:intergenic 35.0%
ATGCACTTGCAAAGTTTACT+TGG + tig0021157:62443-62462 Msa1388680:CDS 35.0%
TGCACTTGCAAAGTTTACTT+GGG + tig0021157:62444-62463 Msa1388680:CDS 35.0%
CTTTCGTTGTTTGCATGATT+TGG + tig0021157:62821-62840 Msa1388680:intron 35.0%
TTCTTGTTAGCTCCAAAAGA+TGG + tig0021157:63038-63057 Msa1388680:intron 35.0%
CAAATCCAATGGATATTGCA+AGG + tig0021157:63067-63086 Msa1388680:CDS 35.0%
TTAGACCTTGCAATATCCAT+TGG - tig0021157:63075-63094 None:intergenic 35.0%
GGATATTGCAAGGTCTAATT+TGG + tig0021157:63077-63096 Msa1388680:CDS 35.0%
AGGTCTAATTTGGCTAAAAG+CGG + tig0021157:63087-63106 Msa1388680:CDS 35.0%
TATCTCTTCTTTGAACCACA+TGG - tig0021157:63138-63157 None:intergenic 35.0%
!! GTTATGTCCACTTGTTATCT+CGG + tig0021157:63284-63303 Msa1388680:CDS 35.0%
CTTTACATCGATCAGCAAAT+AGG + tig0021157:63369-63388 Msa1388680:intron 35.0%
AGGACAAGTATTAAACTGCA+AGG + tig0021157:63787-63806 Msa1388680:CDS 35.0%
TTCCCTTCAGCCTTAATAAA+AGG + tig0021157:64035-64054 Msa1388680:three_prime_UTR 35.0%
! TGCCTTTTATTAAGGCTGAA+GGG - tig0021157:64040-64059 None:intergenic 35.0%
! TTGCCTTTTATTAAGGCTGA+AGG - tig0021157:64041-64060 None:intergenic 35.0%
! TGGGTTCTTGCCTTTTATTA+AGG - tig0021157:64048-64067 None:intergenic 35.0%
GAAAATGAACTTACGGAGTA+AGG - tig0021157:62081-62100 None:intergenic 35.0%
AATGCCTTCGTATTTCTCAA+TGG - tig0021157:62248-62267 None:intergenic 35.0%
ATGCACTTGCAAAGTTTACT+TGG + tig0021157:62443-62462 Msa1388680:CDS 35.0%
TGCACTTGCAAAGTTTACTT+GGG + tig0021157:62444-62463 Msa1388680:CDS 35.0%
CTTTCGTTGTTTGCATGATT+TGG + tig0021157:62821-62840 Msa1388680:intron 35.0%
TTCTTGTTAGCTCCAAAAGA+TGG + tig0021157:63038-63057 Msa1388680:intron 35.0%
CAAATCCAATGGATATTGCA+AGG + tig0021157:63067-63086 Msa1388680:CDS 35.0%
TTAGACCTTGCAATATCCAT+TGG - tig0021157:63075-63094 None:intergenic 35.0%
GGATATTGCAAGGTCTAATT+TGG + tig0021157:63077-63096 Msa1388680:CDS 35.0%
AGGTCTAATTTGGCTAAAAG+CGG + tig0021157:63087-63106 Msa1388680:CDS 35.0%
TATCTCTTCTTTGAACCACA+TGG - tig0021157:63138-63157 None:intergenic 35.0%
!! GTTATGTCCACTTGTTATCT+CGG + tig0021157:63284-63303 Msa1388680:CDS 35.0%
CTTTACATCGATCAGCAAAT+AGG + tig0021157:63369-63388 Msa1388680:intron 35.0%
AGGACAAGTATTAAACTGCA+AGG + tig0021157:63787-63806 Msa1388680:CDS 35.0%
TTCCCTTCAGCCTTAATAAA+AGG + tig0021157:64035-64054 Msa1388680:three_prime_UTR 35.0%
! TGCCTTTTATTAAGGCTGAA+GGG - tig0021157:64040-64059 None:intergenic 35.0%
! TTGCCTTTTATTAAGGCTGA+AGG - tig0021157:64041-64060 None:intergenic 35.0%
! TGGGTTCTTGCCTTTTATTA+AGG - tig0021157:64048-64067 None:intergenic 35.0%
! GTTTTTGTAAGACTGGGAAG+TGG - tig0021157:61851-61870 None:intergenic 40.0%
CTTCCCAGTCTTACAAAAAC+AGG + tig0021157:61851-61870 Msa1388680:five_prime_UTR 40.0%
! GAACCGTAGTGGAAATTGAA+CGG - tig0021157:61971-61990 None:intergenic 40.0%
AAGGTCAAAAACGTCTTCAC+AGG + tig0021157:62014-62033 Msa1388680:CDS 40.0%
AAGGTGAATTGAGATGCATC+TGG - tig0021157:62062-62081 None:intergenic 40.0%
! GCAAACTCTGAGGACAAAAT+AGG - tig0021157:62142-62161 None:intergenic 40.0%
ACTGCCATTGAGAAATACGA+AGG + tig0021157:62241-62260 Msa1388680:CDS 40.0%
TAAGTGCACAATTGCTGAAG+TGG + tig0021157:62417-62436 Msa1388680:CDS 40.0%
TGTTAGCTCCAAAAGATGGT+CGG + tig0021157:63042-63061 Msa1388680:intron 40.0%
!! GATTTGTACCGACCATCTTT+TGG - tig0021157:63053-63072 None:intergenic 40.0%
!! TTTATGAAGGTGCTGTGGAA+AGG + tig0021157:63749-63768 Msa1388680:intron 40.0%
CAAGGAAGCCTTAACTGAAT+GGG + tig0021157:63805-63824 Msa1388680:CDS 40.0%
GTGTAGATCCCATTCAGTTA+AGG - tig0021157:63816-63835 None:intergenic 40.0%
GGCAAGAACCCAAATTGTTA+AGG + tig0021157:64056-64075 Msa1388680:three_prime_UTR 40.0%
ATTTGATCCCTAGCGTAATC+TGG + tig0021157:64115-64134 Msa1388680:three_prime_UTR 40.0%
TTCAAAGCCAGATTACGCTA+GGG - tig0021157:64125-64144 None:intergenic 40.0%
TTTCAAAGCCAGATTACGCT+AGG - tig0021157:64126-64145 None:intergenic 40.0%
! GTTTTTGTAAGACTGGGAAG+TGG - tig0021157:61851-61870 None:intergenic 40.0%
CTTCCCAGTCTTACAAAAAC+AGG + tig0021157:61851-61870 Msa1388680:five_prime_UTR 40.0%
! GAACCGTAGTGGAAATTGAA+CGG - tig0021157:61971-61990 None:intergenic 40.0%
AAGGTCAAAAACGTCTTCAC+AGG + tig0021157:62014-62033 Msa1388680:CDS 40.0%
AAGGTGAATTGAGATGCATC+TGG - tig0021157:62062-62081 None:intergenic 40.0%
! GCAAACTCTGAGGACAAAAT+AGG - tig0021157:62142-62161 None:intergenic 40.0%
ACTGCCATTGAGAAATACGA+AGG + tig0021157:62241-62260 Msa1388680:CDS 40.0%
TAAGTGCACAATTGCTGAAG+TGG + tig0021157:62417-62436 Msa1388680:CDS 40.0%
TGTTAGCTCCAAAAGATGGT+CGG + tig0021157:63042-63061 Msa1388680:intron 40.0%
!! GATTTGTACCGACCATCTTT+TGG - tig0021157:63053-63072 None:intergenic 40.0%
!! TTTATGAAGGTGCTGTGGAA+AGG + tig0021157:63749-63768 Msa1388680:intron 40.0%
CAAGGAAGCCTTAACTGAAT+GGG + tig0021157:63805-63824 Msa1388680:CDS 40.0%
GTGTAGATCCCATTCAGTTA+AGG - tig0021157:63816-63835 None:intergenic 40.0%
GGCAAGAACCCAAATTGTTA+AGG + tig0021157:64056-64075 Msa1388680:three_prime_UTR 40.0%
ATTTGATCCCTAGCGTAATC+TGG + tig0021157:64115-64134 Msa1388680:three_prime_UTR 40.0%
TTCAAAGCCAGATTACGCTA+GGG - tig0021157:64125-64144 None:intergenic 40.0%
TTTCAAAGCCAGATTACGCT+AGG - tig0021157:64126-64145 None:intergenic 40.0%
AAGACTGGGAAGTGGAAAAC+TGG - tig0021157:61843-61862 None:intergenic 45.0%
TAGTGGAAATTGAACGGTGG+TGG - tig0021157:61965-61984 None:intergenic 45.0%
CCACCGTTCAATTTCCACTA+CGG + tig0021157:61965-61984 Msa1388680:CDS 45.0%
TCACCTTCAGAGTTCATGGA+TGG + tig0021157:61989-62008 Msa1388680:CDS 45.0%
TGTCCATCCATGAACTCTGA+AGG - tig0021157:61995-62014 None:intergenic 45.0%
TCAGAGTTCATGGATGGACA+AGG + tig0021157:61995-62014 Msa1388680:CDS 45.0%
TTCACAGGCCAAAAATCCGA+AGG + tig0021157:62029-62048 Msa1388680:CDS 45.0%
GTGAATTGAGATGCATCTGG+TGG - tig0021157:62059-62078 None:intergenic 45.0%
CATTTCATCGGCAAACTCTG+AGG - tig0021157:62152-62171 None:intergenic 45.0%
AATCGAATGCGGCGAGATAT+CGG - tig0021157:62273-62292 None:intergenic 45.0%
CTCTCCTGTGTAATCGAATG+CGG - tig0021157:62284-62303 None:intergenic 45.0%
GATGGTCGGTACAAATCCAA+TGG + tig0021157:63056-63075 Msa1388680:CDS 45.0%
CTAAAAGCGGACAAGTTAGC+AGG + tig0021157:63100-63119 Msa1388680:CDS 45.0%
CTTTGAACCACATGGACAGA+GGG - tig0021157:63130-63149 None:intergenic 45.0%
TCTTTGAACCACATGGACAG+AGG - tig0021157:63131-63150 None:intergenic 45.0%
TAGCAAGCCGAGATAACAAG+TGG - tig0021157:63294-63313 None:intergenic 45.0%
!! TCTCGGCTTGCTAAAGTTCT+TGG + tig0021157:63301-63320 Msa1388680:CDS 45.0%
!! CTCGGCTTGCTAAAGTTCTT+GGG + tig0021157:63302-63321 Msa1388680:CDS 45.0%
!! TCGGCTTGCTAAAGTTCTTG+GGG + tig0021157:63303-63322 Msa1388680:CDS 45.0%
GCAAGGAAGCCTTAACTGAA+TGG + tig0021157:63804-63823 Msa1388680:CDS 45.0%
AATGCACTAGACTGCAGACA+TGG + tig0021157:63871-63890 Msa1388680:exon 45.0%
AAGACTGGGAAGTGGAAAAC+TGG - tig0021157:61843-61862 None:intergenic 45.0%
TAGTGGAAATTGAACGGTGG+TGG - tig0021157:61965-61984 None:intergenic 45.0%
CCACCGTTCAATTTCCACTA+CGG + tig0021157:61965-61984 Msa1388680:CDS 45.0%
TCACCTTCAGAGTTCATGGA+TGG + tig0021157:61989-62008 Msa1388680:CDS 45.0%
TGTCCATCCATGAACTCTGA+AGG - tig0021157:61995-62014 None:intergenic 45.0%
TCAGAGTTCATGGATGGACA+AGG + tig0021157:61995-62014 Msa1388680:CDS 45.0%
TTCACAGGCCAAAAATCCGA+AGG + tig0021157:62029-62048 Msa1388680:CDS 45.0%
GTGAATTGAGATGCATCTGG+TGG - tig0021157:62059-62078 None:intergenic 45.0%
CATTTCATCGGCAAACTCTG+AGG - tig0021157:62152-62171 None:intergenic 45.0%
AATCGAATGCGGCGAGATAT+CGG - tig0021157:62273-62292 None:intergenic 45.0%
CTCTCCTGTGTAATCGAATG+CGG - tig0021157:62284-62303 None:intergenic 45.0%
GATGGTCGGTACAAATCCAA+TGG + tig0021157:63056-63075 Msa1388680:CDS 45.0%
CTAAAAGCGGACAAGTTAGC+AGG + tig0021157:63100-63119 Msa1388680:CDS 45.0%
CTTTGAACCACATGGACAGA+GGG - tig0021157:63130-63149 None:intergenic 45.0%
TCTTTGAACCACATGGACAG+AGG - tig0021157:63131-63150 None:intergenic 45.0%
TAGCAAGCCGAGATAACAAG+TGG - tig0021157:63294-63313 None:intergenic 45.0%
!! TCTCGGCTTGCTAAAGTTCT+TGG + tig0021157:63301-63320 Msa1388680:CDS 45.0%
!! CTCGGCTTGCTAAAGTTCTT+GGG + tig0021157:63302-63321 Msa1388680:CDS 45.0%
!! TCGGCTTGCTAAAGTTCTTG+GGG + tig0021157:63303-63322 Msa1388680:CDS 45.0%
GCAAGGAAGCCTTAACTGAA+TGG + tig0021157:63804-63823 Msa1388680:CDS 45.0%
AATGCACTAGACTGCAGACA+TGG + tig0021157:63871-63890 Msa1388680:exon 45.0%
!!! TGTATTATTTATTTTTATAT+AGG - tig0021157:62735-62754 None:intergenic 5.0%
!!! TGTATTATTTATTTTTATAT+AGG - tig0021157:62735-62754 None:intergenic 5.0%
TCTGTTTGCGATGCGAAGTG+CGG - tig0021157:61904-61923 None:intergenic 50.0%
! CCGTAGTGGAAATTGAACGG+TGG - tig0021157:61968-61987 None:intergenic 50.0%
! ACTCTGAAGGTGAACCGTAG+TGG - tig0021157:61982-62001 None:intergenic 50.0%
CGGTTCACCTTCAGAGTTCA+TGG + tig0021157:61985-62004 Msa1388680:CDS 50.0%
! GTGGTGCTCCTTCGGATTTT+TGG - tig0021157:62040-62059 None:intergenic 50.0%
CGCATTCGATTACACAGGAG+AGG + tig0021157:62282-62301 Msa1388680:CDS 50.0%
CTGCAGTCTAGTGCATTGAG+CGG - tig0021157:63868-63887 None:intergenic 50.0%
TCTGTTTGCGATGCGAAGTG+CGG - tig0021157:61904-61923 None:intergenic 50.0%
! CCGTAGTGGAAATTGAACGG+TGG - tig0021157:61968-61987 None:intergenic 50.0%
! ACTCTGAAGGTGAACCGTAG+TGG - tig0021157:61982-62001 None:intergenic 50.0%
CGGTTCACCTTCAGAGTTCA+TGG + tig0021157:61985-62004 Msa1388680:CDS 50.0%
! GTGGTGCTCCTTCGGATTTT+TGG - tig0021157:62040-62059 None:intergenic 50.0%
CGCATTCGATTACACAGGAG+AGG + tig0021157:62282-62301 Msa1388680:CDS 50.0%
CTGCAGTCTAGTGCATTGAG+CGG - tig0021157:63868-63887 None:intergenic 50.0%
! TGCATCTGGTGGTGCTCCTT+CGG - tig0021157:62048-62067 None:intergenic 55.0%
CTCGCCGCATTCGATTACAC+AGG + tig0021157:62277-62296 Msa1388680:CDS 55.0%
AGGAATGCCCTCTGTCCATG+TGG + tig0021157:63120-63139 Msa1388680:CDS 55.0%
! TGCATCTGGTGGTGCTCCTT+CGG - tig0021157:62048-62067 None:intergenic 55.0%
CTCGCCGCATTCGATTACAC+AGG + tig0021157:62277-62296 Msa1388680:CDS 55.0%
AGGAATGCCCTCTGTCCATG+TGG + tig0021157:63120-63139 Msa1388680:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0021157 gene 61822 64166 61822 ID=Msa1388680;Name=Msa1388680
tig0021157 mRNA 61822 64166 61822 ID=Msa1388680-mRNA-1;Parent=Msa1388680;Name=Msa1388680-mRNA-1;_AED=0.08;_eAED=0.08;_QI=75|0.6|0.83|0.83|0.8|0.83|6|286|263
tig0021157 exon 61822 62086 61822 ID=Msa1388680-mRNA-1:exon:11239;Parent=Msa1388680-mRNA-1
tig0021157 exon 62155 62303 62155 ID=Msa1388680-mRNA-1:exon:11240;Parent=Msa1388680-mRNA-1
tig0021157 exon 62376 62537 62376 ID=Msa1388680-mRNA-1:exon:11241;Parent=Msa1388680-mRNA-1
tig0021157 exon 63048 63163 63048 ID=Msa1388680-mRNA-1:exon:11242;Parent=Msa1388680-mRNA-1
tig0021157 exon 63273 63324 63273 ID=Msa1388680-mRNA-1:exon:11243;Parent=Msa1388680-mRNA-1
tig0021157 exon 63758 64166 63758 ID=Msa1388680-mRNA-1:exon:11244;Parent=Msa1388680-mRNA-1
tig0021157 five_prime_UTR 61822 61896 61822 ID=Msa1388680-mRNA-1:five_prime_utr;Parent=Msa1388680-mRNA-1
tig0021157 CDS 61897 62086 61897 ID=Msa1388680-mRNA-1:cds;Parent=Msa1388680-mRNA-1
tig0021157 CDS 62155 62303 62155 ID=Msa1388680-mRNA-1:cds;Parent=Msa1388680-mRNA-1
tig0021157 CDS 62376 62537 62376 ID=Msa1388680-mRNA-1:cds;Parent=Msa1388680-mRNA-1
tig0021157 CDS 63048 63163 63048 ID=Msa1388680-mRNA-1:cds;Parent=Msa1388680-mRNA-1
tig0021157 CDS 63273 63324 63273 ID=Msa1388680-mRNA-1:cds;Parent=Msa1388680-mRNA-1
tig0021157 CDS 63758 63880 63758 ID=Msa1388680-mRNA-1:cds;Parent=Msa1388680-mRNA-1
tig0021157 three_prime_UTR 63881 64166 63881 ID=Msa1388680-mRNA-1:three_prime_utr;Parent=Msa1388680-mRNA-1
Gene Sequence

>Msa1388680

ATGGCCGCACTTCGCATCGCAAACAGAATCATAAATTATCATCATAATCTCTTATACAACAATTACCACCACCGTTCAATTTCCACTACGGTTCACCTTCAGAGTTCATGGATGGACAAGGTCAAAAACGTCTTCACAGGCCAAAAATCCGAAGGAGCACCACCAGATGCATCTCAATTCACCTTACTCCAGTTTGCCGATGAAATGAAGAAAGCAAGAAAAGTTGGTGCCTTTAAAGAGTATATAGTTGGACGAAGCAGCGAAGTTACTTTTAGTACTGCCATTGAGAAATACGAAGGCATTATCCGATATCTCGCCGCATTCGATTACACAGGAGAGAACTTGACAACGAATCAAAAACATGAAGCGGCGAAACATTGTAAGTGCACAATTGCTGAAGTGGAGAATGCACTTGCAAAGTTTACTTGGGCTAAAGAAGCACACAAGAAGATTCAAAAGTTAAATCAAGAAGGAAAACCCATGCCTAAAAATATGTCTGAACTCCAAAAGATGGTCGGTACAAATCCAATGGATATTGCAAGGTCTAATTTGGCTAAAAGCGGACAAGTTAGCAGGAATGCCCTCTGTCCATGTGGTTCAAAGAAGAGATATAAAAGAAAAGCAAAATGTTATGTCCACTTGTTATCTCGGCTTGCTAAAGTTCTTGGGGTGCTGTGGAAAGGATTGAAAGAATTCAGAGGACAAGTATTAAACTGCAAGGAAGCCTTAACTGAATGGGATCTACACTTGTACTATTTTATCTGGTTCAGATCAAGCCGCTCAATGCACTAG
Protein sequence

>Msa1388680

MAALRIANRIINYHHNLLYNNYHHRSISTTVHLQSSWMDKVKNVFTGQKSEGAPPDASQFTLLQFADEMKKARKVGAFKEYIVGRSSEVTFSTAIEKYEGIIRYLAAFDYTGENLTTNQKHEAAKHCKCTIAEVENALAKFTWAKEAHKKIQKLNQEGKPMPKNMSELQKMVGTNPMDIARSNLAKSGQVSRNALCPCGSKKRYKRKAKCYVHLLSRLAKVLGVLWKGLKEFRGQVLNCKEALTEWDLHLYYFIWFRSSRSMH*