AlfalfaGEDB | Msa1173250

M. sativa cultivar zhongmu-4 / Msa1173250

KEGG: K20556
GO: GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1173250	XP_013469342.2	94.141	256	15	0	1	256	1	256	2.19e-176	505

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1173250	sp\|Q8VWZ7\|C76B6_CATRO	39.033	269	157	3	1	266	1	265	3.57e-58	196

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0035650	Msa1173250	0.896207	4.604974e-76	-8.615850e-47
Msa0035660	Msa1173250	0.813350	2.676767e-51	-8.615850e-47
Msa0035670	Msa1173250	0.967043	9.780539e-127	-8.615850e-47
Msa0035690	Msa1173250	0.869400	3.185360e-66	-8.615850e-47
Msa0035710	Msa1173250	0.835459	1.657889e-56	-8.615850e-47
Msa0048510	Msa1173250	0.804815	1.801852e-49	-8.615850e-47
Msa0077560	Msa1173250	0.820785	5.707561e-53	-8.615850e-47
Msa0083730	Msa1173250	0.815263	1.010886e-51	-8.615850e-47
Msa0083750	Msa1173250	0.886031	4.871694e-72	-8.615850e-47
Msa0083800	Msa1173250	0.830935	2.218545e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1173250	MtrunA17_Chr1g0197851	94.141	256	15	0	1	256	1	256	2.01e-180	505
Msa1173250	MtrunA17_Chr1g0197881	93.750	256	15	1	1	256	1	255	2.65e-177	497
Msa1173250	MtrunA17_Chr1g0197891	92.578	256	19	0	1	256	1	256	5.42e-176	494
Msa1173250	MtrunA17_Chr1g0197871	83.203	256	43	0	1	256	1	256	3.59e-158	449
Msa1173250	MtrunA17_Chr2g0314731	55.814	258	111	2	1	256	1	257	8.93e-92	280
Msa1173250	MtrunA17_Chr2g0314721	53.516	256	117	1	3	256	13	268	7.08e-89	272
Msa1173250	MtrunA17_Chr1g0176831	51.953	256	121	1	3	256	32	287	1.16e-85	265
Msa1173250	MtrunA17_Chr1g0205361	46.124	258	137	1	1	256	1	258	3.91e-83	258
Msa1173250	MtrunA17_Chr1g0205371	43.609	266	147	2	1	263	1	266	7.17e-82	254
Msa1173250	MtrunA17_Chr1g0160851	45.560	259	139	1	1	257	1	259	1.74e-81	253

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1173250	AT3G61040.2	33.061	245	158	2	25	264	24	267	6.11e-44	154
Msa1173250	AT3G61040.1	33.061	245	158	2	25	264	24	267	3.60e-43	154
Msa1173250	AT2G45550.1	30.357	280	181	5	1	266	1	280	1.85e-40	146
Msa1173250	AT2G45560.2	31.535	241	156	3	1	232	1	241	3.93e-38	136
Msa1173250	AT2G45560.3	31.250	240	156	3	1	231	1	240	6.08e-38	134
Msa1173250	AT2G45560.1	31.535	241	156	3	1	232	1	241	1.54e-36	136
Msa1173250	AT2G45580.3	32.719	217	142	1	44	256	48	264	2.24e-34	128
Msa1173250	AT2G45580.1	32.719	217	142	1	44	256	48	264	6.37e-34	129
Msa1173250	AT3G52970.1	27.984	243	170	3	8	245	10	252	2.26e-33	127
Msa1173250	AT2G45570.1	31.439	264	173	4	1	256	1	264	3.17e-32	124

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Find 28 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACTTGCAAAGTTTGCTA+AGG	0.524211	8_1:+3521450	Msa1173250:CDS
AACTTTGCAAGTGTTTGTTG+TGG	0.637386	8_1:-3521442	None:intergenic
AATCATCTCATCATCACATA+TGG	0.458125	8_1:+3521519	Msa1173250:CDS
ACCGGTGAAGCTGTTGATGT+TGG	0.556936	8_1:+3521781	Msa1173250:CDS
AGTTGTAGTGTTATCAGGAA+CGG	0.594813	8_1:-3521587	None:intergenic
ATGCATAAAAGCAGCTTAAC+CGG	0.422718	8_1:+3521763	Msa1173250:CDS
ATGTGATGATGAGATGATTA+TGG	0.455610	8_1:-3521515	None:intergenic
ATTTCAGATATGGATTATGT+TGG	0.514460	8_1:+3521274	None:intergenic
CACCTCTTTGGCAACATCTT+AGG	0.253021	8_1:+3521650	Msa1173250:CDS
CATAACTTAATGCGCATAAT+AGG	0.313177	8_1:-3521481	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATAACTTAATGCGCATAAT+AGG	-	chr8_1:3521484-3521503	None:intergenic	30.0%
AATCATCTCATCATCACATA+TGG	+	chr8_1:3521519-3521538	Msa1173250:CDS	30.0%
AAAAAATTGGGAATAGAGAC+GGG	+	chr8_1:3522061-3522080	Msa1173250:CDS	30.0%
CATAACTTAATGCGCATAAT+AGG	-	chr8_1:3521484-3521503	None:intergenic	30.0%
AATCATCTCATCATCACATA+TGG	+	chr8_1:3521519-3521538	Msa1173250:CDS	30.0%
AAAAAATTGGGAATAGAGAC+GGG	+	chr8_1:3522061-3522080	Msa1173250:CDS	30.0%
TGACAATGCATGTCAACAAA+AGG	-	chr8_1:3521312-3521331	None:intergenic	35.0%
TGCATTGTCATGTTCTTCAT+TGG	+	chr8_1:3521322-3521341	Msa1173250:CDS	35.0%
ATAGTGAAAAAAGAAGGTCC+TGG	-	chr8_1:3521391-3521410	None:intergenic	35.0%
AGTTGTAGTGTTATCAGGAA+CGG	-	chr8_1:3521590-3521609	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	3521283	3522104	3521283	ID=Msa1173250;Name=Msa1173250
chr8_1	mRNA	3521283	3522104	3521283	ID=Msa1173250-mRNA-1;Parent=Msa1173250;Name=Msa1173250-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|273
chr8_1	exon	3521283	3522104	3521283	ID=Msa1173250-mRNA-1:exon:1261;Parent=Msa1173250-mRNA-1
chr8_1	CDS	3521283	3522104	3521283	ID=Msa1173250-mRNA-1:cds;Parent=Msa1173250-mRNA-1

Gene Sequence

>Msa1173250

ATGGATTATGTTGGTAGTGTTATGCTCCTTTTGTTGACATGCATTGTCATGTTCTTCATTGGTTCACTACATGCAAGAAGCAAAAAATCGAACTACAAGCTTCCACCAGGACCTTCTTTTTTCACTATCATGTCACATGTTGTTGAATTGTACAACAAGCCACAACAAACACTTGCAAAGTTTGCTAAGGTCTATGGTCCTATTATGCGCATTAAGTTATGCACAGAAACTACCATAATCATCTCATCATCACATATGGCCAAAGAGATTCTTTTTACAAATGATTCATTGTTCACTGATAGATCCGTTCCTGATAACACTACAACTCACAATCACAACAATTTTAGCTTAGTTTTCCTCCCATTTTCACCTCTTTGGCAACATCTTAGGAAAATATGTCATAATAATTTATTCTCTACCAAGACTCTTGATGGAAGTCAAGAACTTAGACGTATGAAATTAAAAGATCTTCTCAATGATATGCATAAAAGCAGCTTAACCGGTGAAGCTGTTGATGTTGGAAGAGCTGCTTTCAAGGCTTGCATTAATTTTTTGTCATACACTTTTGTGTCTCAAGATTTTGTTGAGAATTTAGATGATGAATATAAAAATATAGTGTCAACTCTATTAAGTGCTGTTGGAACACCAAACATTGCTGATCATTTCCCAGTGTTGAAGATTTTTGACCCACAAGGCATTAGAAGACACACTACTCGTTATGTTTCAAAAGTGTTCTATGCTTTGGATATCATAATTGACAAACGAATGAACACTACTAAAAAAATTGGGAATAGAGACGGGCAAAATCCGTCTCTAAAATGA

Protein sequence

>Msa1173250

MDYVGSVMLLLLTCIVMFFIGSLHARSKKSNYKLPPGPSFFTIMSHVVELYNKPQQTLAKFAKVYGPIMRIKLCTETTIIISSSHMAKEILFTNDSLFTDRSVPDNTTTHNHNNFSLVFLPFSPLWQHLRKICHNNLFSTKTLDGSQELRRMKLKDLLNDMHKSSLTGEAVDVGRAAFKACINFLSYTFVSQDFVENLDDEYKNIVSTLLSAVGTPNIADHFPVLKIFDPQGIRRHTTRYVSKVFYALDIIIDKRMNTTKKIGNRDGQNPSLK*