AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1263050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1263050 MtrunA17_Chr4g0075751 92.857 140 9 1 1 139 1 140 5.87e-41 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCACTGCTACTACTTCTT+CGG 0.329034 8_2:-66136212 None:intergenic
TTCTTCAACTGTTGCTGCTA+TGG 0.377581 8_2:-66136461 None:intergenic
TTACCTCAACAGTGGCCATT+TGG 0.390008 8_2:-66135597 None:intergenic
TGTGTACTCTCAGCTTCAAC+TGG 0.435451 8_2:-66136253 None:intergenic
TGCTACTACTTCTTCGGTGG+TGG 0.459750 8_2:-66136206 None:intergenic
ACTACTTCTTCGGTGGTGGC+TGG 0.461076 8_2:-66136202 None:intergenic
CACAAGTAACTGAGGAAGCC+AGG 0.463325 8_2:+66136272 Msa1263050:CDS
CGGGTTCTCTACCTCTGCCC+TGG 0.474403 8_2:-66136290 None:intergenic
ATTCATCTTTCCATTCCAAA+TGG 0.477645 8_2:+66135582 Msa1263050:exon
CGGTGTTGTTTCCTCGACCT+TGG 0.487832 8_2:-66136155 None:intergenic
AGAAACTACTGAAACAGAAT+CGG 0.495909 8_2:+66136363 Msa1263050:CDS
GAAGTAGTAGCAGTGGAGAA+AGG 0.498978 8_2:+66136217 Msa1263050:CDS
CAGGGCAGAGGTAGAGAACC+CGG 0.499260 8_2:+66136291 Msa1263050:CDS
GGTGGTGGCTGGTTCTGAAG+TGG 0.534204 8_2:-66136191 None:intergenic
AGAGGAAGTATCAGCTGAAG+AGG 0.536609 8_2:+66136336 Msa1263050:CDS
ATTCCAAATGGCCACTGTTG+AGG 0.536707 8_2:+66135594 Msa1263050:exon
AACTGAGGAAGCCAGGGCAG+AGG 0.537053 8_2:+66136279 Msa1263050:CDS
TCAACAGTGGCCATTTGGAA+TGG 0.543349 8_2:-66135592 None:intergenic
GTGGTGGCTGGTTCTGAAGT+GGG 0.546159 8_2:-66136190 None:intergenic
CACCGAAGAAGTAGTAGCAG+TGG 0.549057 8_2:+66136210 Msa1263050:CDS
GAAGTGGGAACCTCGGAGGC+CGG 0.549296 8_2:-66136175 None:intergenic
AGAAGAAGTATCAACTGAAG+AGG 0.562005 8_2:+66136408 Msa1263050:CDS
CACTGCTACTACTTCTTCGG+TGG 0.564441 8_2:-66136209 None:intergenic
TGGTTCTGAAGTGGGAACCT+CGG 0.596316 8_2:-66136182 None:intergenic
AGTTGAGGTAACCAAGGTCG+AGG 0.604857 8_2:+66136144 Msa1263050:CDS
GACAGAAAATGAAACAGTTG+AGG 0.607247 8_2:+66136129 Msa1263050:CDS
ACAAGTAACTGAGGAAGCCA+GGG 0.632141 8_2:+66136273 Msa1263050:CDS
TGAGAGTACACAAGTAACTG+AGG 0.633074 8_2:+66136264 Msa1263050:CDS
TGAAACAGTTGAGGTAACCA+AGG 0.643241 8_2:+66136138 Msa1263050:CDS
TTCTGAAGTGGGAACCTCGG+AGG 0.645606 8_2:-66136179 None:intergenic
GGAAACAACACCGGCCTCCG+AGG 0.649162 8_2:+66136165 Msa1263050:CDS
TCTTTGAATTACCTCAACAG+TGG 0.681859 8_2:-66135605 None:intergenic
CAAGGTCGAGGAAACAACAC+CGG 0.700669 8_2:+66136156 Msa1263050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGATTATTGATCTTTTT+TGG - chr8_2:66135874-66135893 None:intergenic 15.0%
!!! AATTGATTATTGATCTTTTT+TGG - chr8_2:66135874-66135893 None:intergenic 15.0%
!! AAACAAAAATATTAGTCCAA+TGG + chr8_2:66135794-66135813 Msa1263050:intron 20.0%
!!! ATTTGTTGTTTATAATCCAT+TGG - chr8_2:66135813-66135832 None:intergenic 20.0%
!! TGGATTATGAAAAATAAACA+CGG - chr8_2:66135854-66135873 None:intergenic 20.0%
!! AAACAAAAATATTAGTCCAA+TGG + chr8_2:66135794-66135813 Msa1263050:intron 20.0%
!!! ATTTGTTGTTTATAATCCAT+TGG - chr8_2:66135813-66135832 None:intergenic 20.0%
!! TGGATTATGAAAAATAAACA+CGG - chr8_2:66135854-66135873 None:intergenic 20.0%
!!! AATATTTTTGTTTGATCCAC+TGG - chr8_2:66135787-66135806 None:intergenic 25.0%
! GATTATGAAAAATAAACACG+GGG - chr8_2:66135852-66135871 None:intergenic 25.0%
! GGATTATGAAAAATAAACAC+GGG - chr8_2:66135853-66135872 None:intergenic 25.0%
!!! AATATTTTTGTTTGATCCAC+TGG - chr8_2:66135787-66135806 None:intergenic 25.0%
! GATTATGAAAAATAAACACG+GGG - chr8_2:66135852-66135871 None:intergenic 25.0%
! GGATTATGAAAAATAAACAC+GGG - chr8_2:66135853-66135872 None:intergenic 25.0%
ATTCATCTTTCCATTCCAAA+TGG + chr8_2:66135582-66135601 Msa1263050:exon 30.0%
ATATGACCAAGTAACTCATA+GGG - chr8_2:66136027-66136046 None:intergenic 30.0%
TATATGACCAAGTAACTCAT+AGG - chr8_2:66136028-66136047 None:intergenic 30.0%
TAACAAAACATGTTGAGCAT+TGG + chr8_2:66136052-66136071 Msa1263050:intron 30.0%
AGAAACTACTGAAACAGAAT+CGG + chr8_2:66136363-66136382 Msa1263050:CDS 30.0%
ATTCATCTTTCCATTCCAAA+TGG + chr8_2:66135582-66135601 Msa1263050:exon 30.0%
ATATGACCAAGTAACTCATA+GGG - chr8_2:66136027-66136046 None:intergenic 30.0%
TATATGACCAAGTAACTCAT+AGG - chr8_2:66136028-66136047 None:intergenic 30.0%
TAACAAAACATGTTGAGCAT+TGG + chr8_2:66136052-66136071 Msa1263050:intron 30.0%
AGAAACTACTGAAACAGAAT+CGG + chr8_2:66136363-66136382 Msa1263050:CDS 30.0%
TCTTTGAATTACCTCAACAG+TGG - chr8_2:66135608-66135627 None:intergenic 35.0%
! GCATTGGTGGTTAATGAAAA+TGG + chr8_2:66136068-66136087 Msa1263050:intron 35.0%
TTAATGAAAATGGATCCTGC+AGG + chr8_2:66136078-66136097 Msa1263050:intron 35.0%
! TCATTTTCTGTCACAGTTGT+TGG - chr8_2:66136121-66136140 None:intergenic 35.0%
GACAGAAAATGAAACAGTTG+AGG + chr8_2:66136129-66136148 Msa1263050:CDS 35.0%
!! TCTTTCACTTCGACTTTTTC+AGG - chr8_2:66136319-66136338 None:intergenic 35.0%
TGAAAAAGTCGAAGTGAAAG+AGG + chr8_2:66136318-66136337 Msa1263050:CDS 35.0%
!! TCTTTTGCTTCGACTTTTTC+AGG - chr8_2:66136391-66136410 None:intergenic 35.0%
!! AGAAGAAGTATCAACTGAAG+AGG + chr8_2:66136408-66136427 Msa1263050:CDS 35.0%
TCTTTGAATTACCTCAACAG+TGG - chr8_2:66135608-66135627 None:intergenic 35.0%
! GCATTGGTGGTTAATGAAAA+TGG + chr8_2:66136068-66136087 Msa1263050:intron 35.0%
TTAATGAAAATGGATCCTGC+AGG + chr8_2:66136078-66136097 Msa1263050:intron 35.0%
! TCATTTTCTGTCACAGTTGT+TGG - chr8_2:66136121-66136140 None:intergenic 35.0%
GACAGAAAATGAAACAGTTG+AGG + chr8_2:66136129-66136148 Msa1263050:CDS 35.0%
!! TCTTTCACTTCGACTTTTTC+AGG - chr8_2:66136319-66136338 None:intergenic 35.0%
TGAAAAAGTCGAAGTGAAAG+AGG + chr8_2:66136318-66136337 Msa1263050:CDS 35.0%
!! TCTTTTGCTTCGACTTTTTC+AGG - chr8_2:66136391-66136410 None:intergenic 35.0%
!! AGAAGAAGTATCAACTGAAG+AGG + chr8_2:66136408-66136427 Msa1263050:CDS 35.0%
! ATTCACACAGCATTTTCCAG+TGG + chr8_2:66135768-66135787 Msa1263050:intron 40.0%
CATGTTCCCTATGAGTTACT+TGG + chr8_2:66136018-66136037 Msa1263050:intron 40.0%
!! CAAAACATGTTGAGCATTGG+TGG + chr8_2:66136055-66136074 Msa1263050:intron 40.0%
TGAAACAGTTGAGGTAACCA+AGG + chr8_2:66136138-66136157 Msa1263050:CDS 40.0%
! TGAGAGTACACAAGTAACTG+AGG + chr8_2:66136264-66136283 Msa1263050:CDS 40.0%
!!! ACTTCGACTTTTTCAGGTTC+CGG - chr8_2:66136313-66136332 None:intergenic 40.0%
TTCTTCAACTGTTGCTGCTA+TGG - chr8_2:66136464-66136483 None:intergenic 40.0%
! ATTCACACAGCATTTTCCAG+TGG + chr8_2:66135768-66135787 Msa1263050:intron 40.0%
CATGTTCCCTATGAGTTACT+TGG + chr8_2:66136018-66136037 Msa1263050:intron 40.0%
!! CAAAACATGTTGAGCATTGG+TGG + chr8_2:66136055-66136074 Msa1263050:intron 40.0%
TGAAACAGTTGAGGTAACCA+AGG + chr8_2:66136138-66136157 Msa1263050:CDS 40.0%
! TGAGAGTACACAAGTAACTG+AGG + chr8_2:66136264-66136283 Msa1263050:CDS 40.0%
!!! ACTTCGACTTTTTCAGGTTC+CGG - chr8_2:66136313-66136332 None:intergenic 40.0%
TTCTTCAACTGTTGCTGCTA+TGG - chr8_2:66136464-66136483 None:intergenic 40.0%
TCAACAGTGGCCATTTGGAA+TGG - chr8_2:66135595-66135614 None:intergenic 45.0%
ATTCCAAATGGCCACTGTTG+AGG + chr8_2:66135594-66135613 Msa1263050:exon 45.0%
TTACCTCAACAGTGGCCATT+TGG - chr8_2:66135600-66135619 None:intergenic 45.0%
CTCCACTGCTACTACTTCTT+CGG - chr8_2:66136215-66136234 None:intergenic 45.0%
GAAGTAGTAGCAGTGGAGAA+AGG + chr8_2:66136217-66136236 Msa1263050:CDS 45.0%
TGTGTACTCTCAGCTTCAAC+TGG - chr8_2:66136256-66136275 None:intergenic 45.0%
ACAAGTAACTGAGGAAGCCA+GGG + chr8_2:66136273-66136292 Msa1263050:CDS 45.0%
!!! CTTCGACTTTTTCAGGTTCC+GGG - chr8_2:66136312-66136331 None:intergenic 45.0%
!! AGAGGAAGTATCAGCTGAAG+AGG + chr8_2:66136336-66136355 Msa1263050:CDS 45.0%
TCAACAGTGGCCATTTGGAA+TGG - chr8_2:66135595-66135614 None:intergenic 45.0%
ATTCCAAATGGCCACTGTTG+AGG + chr8_2:66135594-66135613 Msa1263050:exon 45.0%
TTACCTCAACAGTGGCCATT+TGG - chr8_2:66135600-66135619 None:intergenic 45.0%
CTCCACTGCTACTACTTCTT+CGG - chr8_2:66136215-66136234 None:intergenic 45.0%
GAAGTAGTAGCAGTGGAGAA+AGG + chr8_2:66136217-66136236 Msa1263050:CDS 45.0%
TGTGTACTCTCAGCTTCAAC+TGG - chr8_2:66136256-66136275 None:intergenic 45.0%
ACAAGTAACTGAGGAAGCCA+GGG + chr8_2:66136273-66136292 Msa1263050:CDS 45.0%
!!! CTTCGACTTTTTCAGGTTCC+GGG - chr8_2:66136312-66136331 None:intergenic 45.0%
!! AGAGGAAGTATCAGCTGAAG+AGG + chr8_2:66136336-66136355 Msa1263050:CDS 45.0%
! CTTGCTGTGTTTCAACCTGC+AGG - chr8_2:66136096-66136115 None:intergenic 50.0%
AGTTGAGGTAACCAAGGTCG+AGG + chr8_2:66136144-66136163 Msa1263050:CDS 50.0%
CAAGGTCGAGGAAACAACAC+CGG + chr8_2:66136156-66136175 Msa1263050:CDS 50.0%
!! TGGTTCTGAAGTGGGAACCT+CGG - chr8_2:66136185-66136204 None:intergenic 50.0%
TGCTACTACTTCTTCGGTGG+TGG - chr8_2:66136209-66136228 None:intergenic 50.0%
CACTGCTACTACTTCTTCGG+TGG - chr8_2:66136212-66136231 None:intergenic 50.0%
CACCGAAGAAGTAGTAGCAG+TGG + chr8_2:66136210-66136229 Msa1263050:CDS 50.0%
CACAAGTAACTGAGGAAGCC+AGG + chr8_2:66136272-66136291 Msa1263050:CDS 50.0%
! CTTGCTGTGTTTCAACCTGC+AGG - chr8_2:66136096-66136115 None:intergenic 50.0%
AGTTGAGGTAACCAAGGTCG+AGG + chr8_2:66136144-66136163 Msa1263050:CDS 50.0%
CAAGGTCGAGGAAACAACAC+CGG + chr8_2:66136156-66136175 Msa1263050:CDS 50.0%
!! TGGTTCTGAAGTGGGAACCT+CGG - chr8_2:66136185-66136204 None:intergenic 50.0%
TGCTACTACTTCTTCGGTGG+TGG - chr8_2:66136209-66136228 None:intergenic 50.0%
CACTGCTACTACTTCTTCGG+TGG - chr8_2:66136212-66136231 None:intergenic 50.0%
CACCGAAGAAGTAGTAGCAG+TGG + chr8_2:66136210-66136229 Msa1263050:CDS 50.0%
CACAAGTAACTGAGGAAGCC+AGG + chr8_2:66136272-66136291 Msa1263050:CDS 50.0%
!! CGGTGTTGTTTCCTCGACCT+TGG - chr8_2:66136158-66136177 None:intergenic 55.0%
!! TTCTGAAGTGGGAACCTCGG+AGG - chr8_2:66136182-66136201 None:intergenic 55.0%
!! GTGGTGGCTGGTTCTGAAGT+GGG - chr8_2:66136193-66136212 None:intergenic 55.0%
! ACTACTTCTTCGGTGGTGGC+TGG - chr8_2:66136205-66136224 None:intergenic 55.0%
!! CGGTGTTGTTTCCTCGACCT+TGG - chr8_2:66136158-66136177 None:intergenic 55.0%
!! TTCTGAAGTGGGAACCTCGG+AGG - chr8_2:66136182-66136201 None:intergenic 55.0%
!! GTGGTGGCTGGTTCTGAAGT+GGG - chr8_2:66136193-66136212 None:intergenic 55.0%
! ACTACTTCTTCGGTGGTGGC+TGG - chr8_2:66136205-66136224 None:intergenic 55.0%
!! GGTGGTGGCTGGTTCTGAAG+TGG - chr8_2:66136194-66136213 None:intergenic 60.0%
AACTGAGGAAGCCAGGGCAG+AGG + chr8_2:66136279-66136298 Msa1263050:CDS 60.0%
CAGGGCAGAGGTAGAGAACC+CGG + chr8_2:66136291-66136310 Msa1263050:CDS 60.0%
!! GGTGGTGGCTGGTTCTGAAG+TGG - chr8_2:66136194-66136213 None:intergenic 60.0%
AACTGAGGAAGCCAGGGCAG+AGG + chr8_2:66136279-66136298 Msa1263050:CDS 60.0%
CAGGGCAGAGGTAGAGAACC+CGG + chr8_2:66136291-66136310 Msa1263050:CDS 60.0%
GGAAACAACACCGGCCTCCG+AGG + chr8_2:66136165-66136184 Msa1263050:CDS 65.0%
GAAGTGGGAACCTCGGAGGC+CGG - chr8_2:66136178-66136197 None:intergenic 65.0%
CGGGTTCTCTACCTCTGCCC+TGG - chr8_2:66136293-66136312 None:intergenic 65.0%
GGAAACAACACCGGCCTCCG+AGG + chr8_2:66136165-66136184 Msa1263050:CDS 65.0%
GAAGTGGGAACCTCGGAGGC+CGG - chr8_2:66136178-66136197 None:intergenic 65.0%
CGGGTTCTCTACCTCTGCCC+TGG - chr8_2:66136293-66136312 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 66135514 66136504 66135514 ID=Msa1263050;Name=Msa1263050
chr8_2 mRNA 66135514 66136504 66135514 ID=Msa1263050-mRNA-1;Parent=Msa1263050;Name=Msa1263050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=87|1|0.5|1|1|1|2|0|139
chr8_2 exon 66135514 66135615 66135514 ID=Msa1263050-mRNA-1:exon:21534;Parent=Msa1263050-mRNA-1
chr8_2 exon 66136100 66136504 66136100 ID=Msa1263050-mRNA-1:exon:21535;Parent=Msa1263050-mRNA-1
chr8_2 five_prime_UTR 66135514 66135600 66135514 ID=Msa1263050-mRNA-1:five_prime_utr;Parent=Msa1263050-mRNA-1
chr8_2 CDS 66135601 66135615 66135601 ID=Msa1263050-mRNA-1:cds;Parent=Msa1263050-mRNA-1
chr8_2 CDS 66136100 66136504 66136100 ID=Msa1263050-mRNA-1:cds;Parent=Msa1263050-mRNA-1
Gene Sequence

>Msa1263050

ATGGCCACTGTTGAGGTTGAAACACAGCAAGCACCAACAACTGTGACAGAAAATGAAACAGTTGAGGTAACCAAGGTCGAGGAAACAACACCGGCCTCCGAGGTTCCCACTTCAGAACCAGCCACCACCGAAGAAGTAGTAGCAGTGGAGAAAGGAGAAACTGAAGTTCCAGTTGAAGCTGAGAGTACACAAGTAACTGAGGAAGCCAGGGCAGAGGTAGAGAACCCGGAACCTGAAAAAGTCGAAGTGAAAGAGGAAGTATCAGCTGAAGAGGCTAAAGAAACTACTGAAACAGAATCGGCACCTGAAAAAGTCGAAGCAAAAGAAGAAGTATCAACTGAAGAGGCTAAAGAAACTACTGAAACAGAATCAGCAGCCATAGCAGCAACAGTTGAAGAAGTAACCACCACAGAAGCATAA

Protein sequence

>Msa1263050

MATVEVETQQAPTTVTENETVEVTKVEETTPASEVPTSEPATTEEVVAVEKGETEVPVEAESTQVTEEARAEVENPEPEKVEVKEEVSAEEAKETTETESAPEKVEAKEEVSTEEAKETTETESAAIAATVEEVTTTEA*