AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1220720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1220720 MtrunA17_Chr4g0072091 99.071 323 3 0 1 323 1 323 0.0 659
Msa1220720 MtrunA17_Chr5g0401431 65.031 326 108 3 1 320 1 326 8.38e-157 441
Msa1220720 MtrunA17_Chr4g0053871 67.619 315 94 2 9 320 12 321 5.47e-155 437
Msa1220720 MtrunA17_Chr1g0148021 63.240 321 116 1 5 323 8 328 8.46e-148 419
Msa1220720 MtrunA17_Chr7g0225221 55.521 326 136 4 1 323 1 320 4.16e-125 361
Msa1220720 MtrunA17_Chr1g0197411 42.547 322 178 3 7 323 11 330 1.02e-87 266
Msa1220720 MtrunA17_Chr4g0046131 44.375 320 167 5 10 320 26 343 2.46e-85 260
Msa1220720 MtrunA17_Chr8g0364291 42.415 323 179 4 1 319 1 320 4.46e-83 254
Msa1220720 MtrunA17_Chr3g0128061 42.500 320 175 5 5 319 7 322 2.27e-82 252
Msa1220720 MtrunA17_Chr5g0405991 43.614 321 167 8 7 323 6 316 9.68e-82 250
Msa1220720 MtrunA17_Chr6g0451571 42.368 321 176 6 8 323 18 334 2.48e-79 244
Msa1220720 MtrunA17_Chr2g0292211 44.582 323 169 5 3 319 19 337 3.06e-79 245
Msa1220720 MtrunA17_Chr1g0157051 41.139 316 175 5 7 319 7 314 6.87e-79 243
Msa1220720 MtrunA17_Chr7g0258811 41.994 331 180 5 1 323 45 371 3.79e-78 243
Msa1220720 MtrunA17_Chr2g0292241 45.277 307 158 5 19 319 24 326 1.97e-77 240
Msa1220720 MtrunA17_Chr2g0322861 41.250 320 180 5 8 323 71 386 4.61e-77 241
Msa1220720 MtrunA17_Chr1g0175561 41.104 326 178 4 5 323 8 326 8.44e-76 235
Msa1220720 MtrunA17_Chr4g0063671 41.975 324 177 5 6 323 2 320 1.40e-75 234
Msa1220720 MtrunA17_Chr2g0292181 43.648 307 163 5 19 319 33 335 2.77e-75 235
Msa1220720 MtrunA17_Chr2g0292251 43.974 307 162 5 19 319 23 325 7.40e-75 234
Msa1220720 MtrunA17_Chr2g0292271 41.159 328 185 4 1 323 6 330 8.12e-75 234
Msa1220720 MtrunA17_Chr2g0299691 41.562 320 178 4 6 319 38 354 2.70e-74 233
Msa1220720 MtrunA17_Chr8g0371381 42.809 299 159 6 24 319 26 315 1.17e-73 229
Msa1220720 MtrunA17_Chr4g0076211 42.282 298 163 5 27 323 44 333 6.74e-73 228
Msa1220720 MtrunA17_Chr2g0292261 43.648 307 163 4 19 319 24 326 8.01e-73 229
Msa1220720 MtrunA17_Chr2g0291751 44.118 306 161 6 20 319 25 326 2.23e-72 228
Msa1220720 MtrunA17_Chr1g0204011 41.139 316 175 6 8 319 10 318 6.47e-72 225
Msa1220720 MtrunA17_Chr4g0024121 39.557 316 187 3 6 319 9 322 1.21e-71 224
Msa1220720 MtrunA17_Chr7g0268501 40.673 327 183 6 1 323 1 320 3.40e-71 223
Msa1220720 MtrunA17_Chr7g0268521 40.673 327 183 6 1 323 1 320 3.40e-71 223
Msa1220720 MtrunA17_Chr2g0292151 41.694 307 169 6 19 319 24 326 1.36e-70 223
Msa1220720 MtrunA17_Chr1g0194151 40.785 331 178 10 5 323 9 333 1.80e-70 222
Msa1220720 MtrunA17_Chr2g0279041 39.394 330 188 6 1 323 1 325 1.97e-70 221
Msa1220720 MtrunA17_Chr4g0013171 39.938 323 181 6 5 323 8 321 2.24e-70 221
Msa1220720 MtrunA17_Chr5g0404721 40.532 301 170 4 28 323 34 330 6.41e-70 220
Msa1220720 MtrunA17_Chr2g0320071 39.748 317 177 5 6 319 8 313 1.06e-69 219
Msa1220720 MtrunA17_Chr2g0320091 39.185 319 177 6 6 319 8 314 1.36e-69 219
Msa1220720 MtrunA17_Chr1g0194271 40.828 338 175 10 5 323 9 340 1.48e-69 220
Msa1220720 MtrunA17_Chr5g0402581 40.439 319 179 7 6 319 10 322 3.47e-69 218
Msa1220720 MtrunA17_Chr4g0070951 40.752 319 179 5 6 319 12 325 4.68e-69 218
Msa1220720 MtrunA17_Chr7g0254231 42.667 300 158 7 22 319 20 307 1.22e-68 216
Msa1220720 MtrunA17_Chr6g0467251 40.184 326 178 6 5 323 13 328 1.37e-68 218
Msa1220720 MtrunA17_Chr5g0438021 37.273 330 188 6 1 323 8 325 4.30e-68 216
Msa1220720 MtrunA17_Chr5g0402571 39.498 319 182 7 6 319 10 322 4.47e-68 216
Msa1220720 MtrunA17_Chr6g0467121 41.554 296 163 4 24 319 74 359 5.16e-68 217
Msa1220720 MtrunA17_Chr1g0164891 40.789 304 164 8 27 320 92 389 2.56e-67 216
Msa1220720 MtrunA17_Chr2g0320101 39.432 317 178 5 6 319 8 313 3.21e-67 213
Msa1220720 MtrunA17_Chr7g0244451 40.994 322 165 7 11 323 11 316 3.70e-67 213
Msa1220720 MtrunA17_Chr2g0292111 40.909 308 170 7 19 319 24 326 4.01e-67 214
Msa1220720 MtrunA17_Chr2g0292101 40.717 307 172 5 19 319 20 322 4.05e-67 214
Msa1220720 MtrunA17_Chr2g0320031 40.439 319 174 7 6 319 8 315 1.64e-66 211
Msa1220720 MtrunA17_Chr2g0292291 42.208 308 167 5 19 319 25 328 2.14e-66 212
Msa1220720 MtrunA17_Chr2g0292091 39.604 303 175 4 22 319 22 321 2.23e-66 212
Msa1220720 MtrunA17_Chr3g0106921 39.755 327 176 8 5 323 7 320 2.26e-66 211
Msa1220720 MtrunA17_Chr1g0188431 37.072 321 185 6 6 323 21 327 2.33e-66 211
Msa1220720 MtrunA17_Chr2g0320061 39.308 318 178 6 6 319 8 314 7.45e-66 209
Msa1220720 MtrunA17_Chr3g0136751 38.750 320 189 4 6 323 8 322 1.04e-65 209
Msa1220720 MtrunA17_Chr8g0372601 39.394 297 171 4 28 319 34 326 1.09e-65 209
Msa1220720 MtrunA17_Chr4g0013181 40.333 300 166 6 28 323 63 353 1.35e-65 210
Msa1220720 MtrunA17_Chr3g0107881 41.463 328 171 10 6 323 8 324 5.07e-65 207
Msa1220720 MtrunA17_Chr4g0038161 40.367 327 173 9 5 323 8 320 5.67e-65 207
Msa1220720 MtrunA17_Chr3g0129051 39.198 324 178 7 7 323 11 322 1.11e-64 207
Msa1220720 MtrunA17_Chr3g0129061 40.625 320 171 7 11 323 15 322 1.21e-64 206
Msa1220720 MtrunA17_Chr6g0467161 39.024 328 180 6 6 323 19 336 1.24e-64 207
Msa1220720 MtrunA17_Chr4g0038151 39.009 323 180 7 8 323 9 321 1.54e-64 206
Msa1220720 MtrunA17_Chr2g0296621 37.037 297 177 6 24 319 50 337 3.87e-64 206
Msa1220720 MtrunA17_Chr7g0254201 38.538 301 172 6 22 319 18 308 4.61e-64 205
Msa1220720 MtrunA17_Chr7g0244461 37.097 310 166 4 21 319 17 308 4.71e-64 205
Msa1220720 MtrunA17_Chr2g0320051 39.308 318 178 6 6 319 8 314 5.08e-64 205
Msa1220720 MtrunA17_Chr2g0278671 40.064 312 171 8 20 323 20 323 1.50e-63 204
Msa1220720 MtrunA17_Chr4g0043391 39.877 326 177 8 5 323 11 324 2.84e-63 203
Msa1220720 MtrunA17_Chr4g0013201 39.308 318 178 5 10 323 32 338 5.69e-63 203
Msa1220720 MtrunA17_Chr4g0038171 38.365 318 180 6 13 323 13 321 2.39e-62 201
Msa1220720 MtrunA17_Chr5g0432441 40.244 328 172 11 6 323 13 326 3.20e-62 201
Msa1220720 MtrunA17_Chr5g0432351 39.697 330 172 10 6 323 7 321 3.28e-62 200
Msa1220720 MtrunA17_Chr4g0068621 37.621 311 183 5 18 323 32 336 5.54e-62 200
Msa1220720 MtrunA17_Chr4g0013721 37.134 307 184 6 16 319 92 392 9.43e-61 199
Msa1220720 MtrunA17_Chr4g0013191 39.935 308 171 7 21 323 42 340 2.39e-60 196
Msa1220720 MtrunA17_Chr6g0460711 37.658 316 185 6 7 319 4 310 5.93e-60 194
Msa1220720 MtrunA17_Chr5g0432341 38.788 330 175 10 6 323 7 321 6.35e-60 194
Msa1220720 MtrunA17_Chr3g0114451 36.810 326 189 5 5 323 3 318 7.54e-60 194
Msa1220720 MtrunA17_Chr5g0432331 38.788 330 175 10 6 323 38 352 3.82e-59 193
Msa1220720 MtrunA17_Chr4g0013821 36.393 305 181 6 20 319 24 320 4.52e-59 193
Msa1220720 MtrunA17_Chr3g0129041 38.650 326 180 7 6 323 9 322 7.06e-59 192
Msa1220720 MtrunA17_Chr5g0400331 37.864 309 178 6 21 319 23 327 3.12e-57 188
Msa1220720 MtrunA17_Chr6g0477071 35.948 306 179 6 21 323 51 342 3.29e-57 188
Msa1220720 MtrunA17_Chr2g0329641 35.061 328 198 6 5 323 14 335 8.92e-57 187
Msa1220720 MtrunA17_Chr1g0155611 37.908 306 180 5 24 323 46 347 1.35e-55 184
Msa1220720 MtrunA17_Chr1g0213061 37.072 321 183 8 6 319 9 317 3.12e-55 182
Msa1220720 MtrunA17_Chr4g0075241 35.505 307 185 6 23 323 28 327 6.22e-55 182
Msa1220720 MtrunA17_Chr7g0224371 34.700 317 194 6 6 319 12 318 3.56e-54 179
Msa1220720 MtrunA17_Chr2g0316791 34.627 335 180 5 22 321 74 404 4.47e-54 182
Msa1220720 MtrunA17_Chr4g0074851 32.530 332 202 7 5 323 6 328 6.37e-54 179
Msa1220720 MtrunA17_Chr2g0316481 35.224 335 178 6 22 321 74 404 9.20e-54 181
Msa1220720 MtrunA17_Chr2g0279061 39.231 260 149 4 68 323 12 266 2.51e-53 176
Msa1220720 MtrunA17_Chr1g0181511 34.768 302 186 5 22 319 25 319 7.01e-53 176
Msa1220720 MtrunA17_Chr1g0181431 34.106 302 188 5 22 319 25 319 1.04e-52 176
Msa1220720 MtrunA17_Chr2g0316191 34.821 336 179 6 22 321 74 405 1.20e-52 178
Msa1220720 MtrunA17_Chr1g0181481 35.099 302 185 5 22 319 25 319 3.59e-52 174
Msa1220720 MtrunA17_Chr2g0316831 34.627 335 180 6 22 321 110 440 5.63e-52 177
Msa1220720 MtrunA17_Chr3g0106901 35.202 321 193 6 6 323 15 323 1.46e-50 170
Msa1220720 MtrunA17_Chr2g0311241 31.367 373 181 4 22 321 58 428 2.69e-50 172
Msa1220720 MtrunA17_Chr2g0311251 33.828 337 179 7 22 321 9 338 1.60e-49 168
Msa1220720 MtrunA17_Chr5g0421411 35.766 274 163 8 55 319 12 281 3.98e-48 163
Msa1220720 MtrunA17_Chr5g0413051 33.968 315 187 9 22 323 71 377 1.45e-47 164
Msa1220720 MtrunA17_Chr5g0432401 35.168 327 148 10 6 321 11 284 1.24e-45 156
Msa1220720 MtrunA17_Chr4g0004591 32.390 318 155 4 24 321 77 354 1.61e-42 150
Msa1220720 MtrunA17_Chr2g0292281 43.902 205 103 3 117 319 18 212 6.98e-42 145
Msa1220720 MtrunA17_Chr2g0316811 37.391 230 120 5 22 231 48 273 4.04e-37 135
Msa1220720 MtrunA17_Chr3g0114461 37.895 190 110 3 135 323 10 192 8.17e-33 120
Msa1220720 MtrunA17_Chr2g0316141 31.597 288 139 8 70 321 11 276 4.16e-29 112
Msa1220720 MtrunA17_Chr2g0316181 31.597 288 139 8 70 321 11 276 4.16e-29 112
Msa1220720 MtrunA17_Chr1g0181501 31.746 189 118 4 135 319 4 185 1.18e-22 93.2
Msa1220720 MtrunA17_Chr5g0432321 38.889 126 65 3 145 268 17 132 6.01e-19 81.6
Msa1220720 MtrunA17_Chr7g0254181 39.552 134 68 3 96 229 33 153 3.33e-17 77.4
Msa1220720 MtrunA17_Chr1g0181491 34.959 123 76 3 22 143 10 129 1.42e-16 75.5
Msa1220720 MtrunA17_Chr3g0114491 40.179 112 61 3 23 131 17 125 6.94e-16 73.6
Msa1220720 MtrunA17_Chr4g0037131 28.467 274 141 10 55 320 32 258 3.89e-15 74.7
Msa1220720 MtrunA17_Chr3g0137751 27.675 271 120 9 55 308 105 316 1.87e-13 70.1
Msa1220720 MtrunA17_Chr3g0104101 30.435 138 89 5 188 319 9 145 2.64e-12 63.9
Msa1220720 MtrunA17_Chr7g0268491 42.683 82 46 1 242 323 17 97 4.36e-12 61.6
Msa1220720 MtrunA17_Chr5g0423911 46.377 69 37 0 254 322 2 70 5.27e-12 62.0
Msa1220720 MtrunA17_Chr6g0468731 43.662 71 36 2 63 133 22 88 8.34e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1220720 AT2G18980.1 73.981 319 82 1 5 323 6 323 2.45e-175 488
Msa1220720 AT4G30170.1 76.238 303 71 1 21 323 24 325 4.28e-172 480
Msa1220720 AT3G49960.1 62.776 317 115 2 6 319 9 325 3.31e-147 417
Msa1220720 AT4G37530.1 65.017 303 103 1 20 319 23 325 8.11e-147 416
Msa1220720 AT5G67400.1 63.924 316 111 2 7 319 10 325 3.62e-144 410
Msa1220720 AT4G37520.1 63.455 301 107 1 22 319 25 325 8.45e-143 406
Msa1220720 AT4G37520.2 62.458 301 107 2 22 319 25 322 1.34e-138 395
Msa1220720 AT4G37530.2 65.385 260 87 1 20 276 23 282 8.28e-127 364
Msa1220720 AT5G14130.1 55.031 318 138 3 8 323 16 330 6.15e-115 335
Msa1220720 AT5G14130.2 55.031 318 138 3 8 323 57 371 1.18e-114 337
Msa1220720 AT4G17690.1 47.039 304 150 4 24 323 26 322 2.09e-89 270
Msa1220720 AT5G47000.1 45.988 324 163 5 6 323 13 330 3.88e-87 265
Msa1220720 AT2G34060.1 42.598 331 171 6 6 319 14 342 1.57e-85 261
Msa1220720 AT5G40150.1 43.042 309 155 5 20 319 28 324 6.69e-80 246
Msa1220720 AT4G11290.1 42.679 321 170 6 9 319 6 322 4.33e-79 244
Msa1220720 AT3G28200.1 41.311 305 167 4 19 319 16 312 1.23e-77 240
Msa1220720 AT1G05240.1 43.144 299 164 3 28 323 30 325 2.60e-77 239
Msa1220720 AT1G05250.1 43.144 299 164 3 28 323 30 325 2.60e-77 239
Msa1220720 AT1G24110.1 41.176 323 183 5 5 323 3 322 3.79e-77 239
Msa1220720 AT5G06730.1 41.121 321 179 5 6 319 13 330 3.92e-77 240
Msa1220720 AT5G06720.1 40.867 323 181 5 8 323 14 333 4.32e-76 237
Msa1220720 AT1G05260.1 40.850 306 173 4 22 323 24 325 6.67e-76 236
Msa1220720 AT3G50990.1 41.290 310 177 4 17 323 34 341 1.72e-75 236
Msa1220720 AT5G42180.1 40.379 317 176 7 9 321 8 315 5.86e-75 233
Msa1220720 AT2G38380.1 42.949 312 171 4 13 319 20 329 1.60e-74 233
Msa1220720 AT5G15180.1 38.838 327 193 4 1 323 6 329 2.67e-74 232
Msa1220720 AT3G01190.1 41.534 313 177 4 12 323 14 321 7.96e-74 230
Msa1220720 AT4G31760.1 41.615 322 175 5 8 323 12 326 1.44e-73 230
Msa1220720 AT5G51890.1 42.903 310 162 8 13 316 15 315 1.69e-73 229
Msa1220720 AT5G19880.1 42.368 321 174 7 11 323 12 329 2.02e-73 229
Msa1220720 AT2G38390.1 41.987 312 174 4 13 319 20 329 3.84e-73 229
Msa1220720 AT3G49120.1 41.534 313 175 4 13 319 21 331 4.70e-73 229
Msa1220720 AT4G36430.1 40.127 314 183 3 13 323 19 330 5.73e-73 229
Msa1220720 AT3G21770.1 41.176 306 172 5 19 320 24 325 7.07e-73 228
Msa1220720 AT3G32980.1 41.853 313 174 4 13 319 20 330 2.65e-72 228
Msa1220720 AT5G66390.1 40.062 322 184 4 7 323 16 333 5.63e-72 226
Msa1220720 AT3G49110.1 41.214 313 176 4 13 319 22 332 8.07e-72 226
Msa1220720 AT2G18150.1 41.000 300 172 3 27 323 39 336 3.66e-71 224
Msa1220720 AT1G71695.1 42.105 304 164 6 24 323 44 339 4.28e-71 224
Msa1220720 AT5G17820.1 40.867 323 180 6 1 323 2 313 5.50e-71 223
Msa1220720 AT5G17820.2 40.867 323 180 6 1 323 55 366 4.99e-70 222
Msa1220720 AT5G05340.1 41.748 309 161 6 22 323 28 324 5.74e-70 221
Msa1220720 AT2G18140.1 40.803 299 172 3 28 323 39 335 1.97e-69 220
Msa1220720 AT2G39040.1 41.558 308 165 8 22 319 44 346 2.74e-69 220
Msa1220720 AT2G41480.1 37.771 323 191 4 6 323 11 328 2.86e-69 219
Msa1220720 AT2G24800.1 39.542 306 176 5 22 323 29 329 2.17e-68 217
Msa1220720 AT5G64110.1 39.329 328 185 7 4 323 9 330 4.96e-68 216
Msa1220720 AT5G64120.1 39.394 297 174 4 27 323 38 328 1.57e-67 214
Msa1220720 AT4G08780.1 39.610 308 178 4 18 319 18 323 8.65e-67 213
Msa1220720 AT4G08770.1 39.550 311 180 4 15 319 15 323 1.08e-66 213
Msa1220720 AT1G14540.1 40.123 324 176 8 7 323 3 315 1.85e-66 211
Msa1220720 AT5G39580.1 39.375 320 181 7 6 323 11 319 1.30e-65 209
Msa1220720 AT5G58400.1 38.838 327 179 8 5 323 12 325 2.98e-64 206
Msa1220720 AT4G16270.1 39.597 298 169 7 27 319 53 344 7.44e-64 206
Msa1220720 AT1G14550.1 39.692 325 178 10 6 323 8 321 2.47e-63 204
Msa1220720 AT5G58390.1 41.538 325 170 9 7 323 4 316 4.13e-63 203
Msa1220720 AT2G22420.1 37.700 313 182 6 12 319 11 315 4.56e-63 203
Msa1220720 AT1G77100.1 39.677 310 171 6 20 323 20 319 5.46e-63 202
Msa1220720 AT1G49570.1 39.355 310 175 7 21 322 45 349 5.67e-63 203
Msa1220720 AT5G19890.1 40.066 302 171 5 22 319 28 323 8.28e-63 202
Msa1220720 AT3G03670.1 37.500 320 196 3 1 319 1 317 1.05e-62 202
Msa1220720 AT1G30870.1 37.624 303 178 6 24 323 51 345 1.30e-62 202
Msa1220720 AT4G33420.1 37.888 322 179 7 9 323 18 325 4.44e-62 200
Msa1220720 AT4G33420.2 37.888 322 179 7 9 323 65 372 2.27e-61 200
Msa1220720 AT4G25980.1 38.065 310 181 6 20 323 22 326 8.59e-61 197
Msa1220720 AT5G24070.1 36.013 311 186 5 16 319 27 331 5.10e-60 196
Msa1220720 AT1G44970.1 38.926 298 172 5 28 319 49 342 7.05e-59 193
Msa1220720 AT2G43480.1 35.048 311 189 5 20 323 31 335 1.76e-58 191
Msa1220720 AT5G64100.1 35.952 331 191 7 6 323 9 331 3.72e-58 191
Msa1220720 AT4G33870.1 32.440 336 189 4 22 321 66 399 5.02e-58 192
Msa1220720 AT5G22410.1 36.164 318 191 7 7 319 11 321 2.98e-56 186
Msa1220720 AT1G34510.1 36.304 303 171 8 22 319 20 305 3.69e-55 182
Msa1220720 AT2G35380.1 35.905 337 179 9 7 319 8 331 2.88e-54 181
Msa1220720 AT4G26010.2 36.562 320 187 8 2 319 28 333 7.20e-54 179
Msa1220720 AT4G26010.1 36.593 317 185 8 5 319 3 305 7.89e-54 179
Msa1220720 AT1G68850.1 34.202 307 186 9 24 319 30 331 6.51e-53 177
Msa1220720 AT2G37130.1 35.197 304 185 6 21 319 27 323 8.09e-52 174
Msa1220720 AT2G37130.2 35.690 297 179 6 28 319 3 292 1.55e-51 172
Msa1220720 AT4G21960.1 31.548 336 208 7 1 323 1 327 1.46e-50 171
Msa1220720 AT3G17070.1 32.934 334 200 9 3 321 13 337 1.26e-42 150
Msa1220720 AT2G35380.2 35.146 239 129 6 96 319 16 243 5.29e-36 130
Msa1220720 AT5G39580.2 42.268 194 101 5 6 198 11 194 1.14e-35 129
Msa1220720 AT2G24800.2 41.071 168 95 2 22 189 29 192 6.26e-33 122
Msa1220720 AT4G25980.2 38.983 177 104 3 19 195 66 238 2.46e-29 114
Msa1220720 AT3G17070.2 32.584 267 159 9 66 321 18 274 2.68e-28 110
Msa1220720 AT4G32320.1 29.057 265 158 11 11 261 75 323 3.34e-12 66.6

Find 86 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAATTCAACATTATAAAA+TGG 0.234921 8_1:-84010378 None:intergenic
AAAGCACTCTCAAAAGCTTT+TGG 0.240385 8_1:-84010930 None:intergenic
ATTCTAGGATCAACTCTTAA+AGG 0.261695 8_1:-84010759 None:intergenic
CTGGTAATCAAGGTGAAATT+AGG 0.279887 8_1:+84010994 Msa1220720:CDS
TCCATGTTTATTGCAATTCT+AGG 0.304893 8_1:-84010774 None:intergenic
GATGGGTCAATTCTACTTCT+TGG 0.306557 8_1:-84010696 None:intergenic
AACAAGAGATGTCGTCAATT+TGG 0.322334 8_1:+84010199 Msa1220720:CDS
ACAGATATGGTTGCATTATC+AGG 0.330722 8_1:+84010513 Msa1220720:CDS
TTCGGAAGCATGCAGTTATA+TGG 0.349386 8_1:+84011074 Msa1220720:three_prime_UTR
TATTAATGCTATTACTAAGT+TGG 0.351024 8_1:+84010953 Msa1220720:CDS
AAGGACACATTTGCCTAAGT+TGG 0.369798 8_1:-84010740 None:intergenic
AGGCCAAGGCAGCCGTTGAT+AGG 0.374458 8_1:+84010126 Msa1220720:CDS
TCTTCCATGATTGCTTTGTC+AGG 0.378203 8_1:+84009893 Msa1220720:CDS
GGAAGGGTTGGTGTTAAAAC+TGG 0.381845 8_1:+84010975 Msa1220720:CDS
ATTAATGCTATTACTAAGTT+GGG 0.389915 8_1:+84010954 Msa1220720:CDS
TGTTTAGGTGCACATACAAT+TGG 0.394824 8_1:+84010627 Msa1220720:intron
TACAATATGCTTTCCAACTT+AGG 0.395009 8_1:+84010727 Msa1220720:CDS
ATGGGTCAATTCTACTTCTT+GGG 0.405954 8_1:-84010695 None:intergenic
CGAACCAATTGTTCAACATT+GGG 0.410150 8_1:-84009808 None:intergenic
TATGGCATCATCGTCGGATT+CGG 0.411722 8_1:+84011056 Msa1220720:three_prime_UTR
GCATATTGTAAATTTAATGA+TGG 0.417152 8_1:-84010714 None:intergenic
AAGTGTTGTTTACTGATTCA+AGG 0.418439 8_1:+84010874 Msa1220720:CDS
AGTTCTTTGAGGTATATTCC+TGG 0.424322 8_1:+84011318 Msa1220720:three_prime_UTR
ATAAATGATCAAGTTCTTTG+AGG 0.429563 8_1:+84011307 Msa1220720:three_prime_UTR
TGGCCTCTCTCAAACAGATA+TGG 0.436444 8_1:+84010500 Msa1220720:CDS
ACCGTTCAGTCTCAAATCAA+TGG 0.436643 8_1:-84011112 None:intergenic
ATCCCTATCAACGGCTGCCT+TGG 0.441611 8_1:-84010129 None:intergenic
GGATGTTCCCTCTCTGCCTT+CGG 0.455966 8_1:-84010059 None:intergenic
GAGACTTTGTTTCGGCACTT+AGG 0.474560 8_1:-84010152 None:intergenic
TTGTTGAAACTTCTGATTCA+CGG 0.478111 8_1:-84009836 None:intergenic
GGTGTTAAAACTGGTAATCA+AGG 0.480424 8_1:+84010984 Msa1220720:CDS
ATGCCCCAATGTTGAACAAT+TGG 0.480960 8_1:+84009804 Msa1220720:CDS
CTTCCATGATTGCTTTGTCA+GGG 0.481760 8_1:+84009894 Msa1220720:CDS
AGCTGATTCAAATTGAAATG+AGG 0.482900 8_1:-84010456 None:intergenic
AAGAACAGTCTGAGAGTGGC+AGG 0.483844 8_1:-84009874 None:intergenic
CAGCACAAGAGACTTTGTTT+CGG 0.486532 8_1:-84010160 None:intergenic
GCTGATTCAAATTGAAATGA+GGG 0.493262 8_1:-84010455 None:intergenic
CGCTTCTCAAATAGAATCTA+CGG 0.494457 8_1:+84010666 Msa1220720:CDS
ATACCCTGACAAAGCAATCA+TGG 0.497957 8_1:-84009897 None:intergenic
GACCATCATATCAAAATCAA+TGG 0.498791 8_1:-84011176 None:intergenic
ACTGCGTCAAGTGGTTATGC+AGG 0.500869 8_1:+84011209 Msa1220720:three_prime_UTR
AATACCATGTTTAATCTCCA+TGG 0.501959 8_1:+84010480 Msa1220720:CDS
CATATTGTAAATTTAATGAT+GGG 0.504769 8_1:-84010713 None:intergenic
ACGAACCAATTGTTCAACAT+TGG 0.505241 8_1:-84009809 None:intergenic
TTCAAATTGAAATGAGGGCC+AGG 0.507134 8_1:-84010450 None:intergenic
GGCCAAGGCAGCCGTTGATA+GGG 0.509062 8_1:+84010127 Msa1220720:CDS
TTTATAATGTTGAATTGGGA+AGG 0.509337 8_1:+84010382 Msa1220720:CDS
AAGAATCTACAACAAGGAAA+AGG 0.514037 8_1:+84010834 Msa1220720:CDS
TGCTATTACTAAGTTGGGAA+GGG 0.516971 8_1:+84010959 Msa1220720:CDS
AACAGAACATTCATTAGCAA+TGG 0.517705 8_1:+84009690 Msa1220720:exon
GTTTACTGATTCAAGGTCAA+AGG 0.518163 8_1:+84010881 Msa1220720:CDS
CACAGTGTCAAAACCGTCCC+CGG 0.519436 8_1:-84010099 None:intergenic
CCCAAAAGAAAAGATGATGA+TGG 0.520472 8_1:-84011242 None:intergenic
CCGGCTAGTGATATATCATC+AGG 0.522576 8_1:-84010080 None:intergenic
AAATTAGGTTTGATTGTACC+AGG 0.524795 8_1:+84011009 Msa1220720:CDS
CGTAGATTCTATTTGAGAAG+CGG 0.527609 8_1:-84010665 None:intergenic
ATTACTAAGTTGGGAAGGGT+TGG 0.533445 8_1:+84010963 Msa1220720:CDS
ATGCTATTACTAAGTTGGGA+AGG 0.537394 8_1:+84010958 Msa1220720:CDS
TTGAATTGGGAAGGCGTGAT+GGG 0.540575 8_1:+84010391 Msa1220720:CDS
GTTGAATTGGGAAGGCGTGA+TGG 0.540741 8_1:+84010390 Msa1220720:CDS
TGACACTGTGGTTAAGGCCA+AGG 0.546297 8_1:+84010112 Msa1220720:CDS
CCTAGTAGTAACTTGAAGCC+AGG 0.547090 8_1:-84011336 None:intergenic
CCTGATGATATATCACTAGC+CGG 0.549221 8_1:+84010080 Msa1220720:CDS
ACCATTGATTTGAGACTGAA+CGG 0.550622 8_1:+84011111 Msa1220720:three_prime_UTR
TACTGTTATGGCATCATCGT+CGG 0.553428 8_1:+84011050 Msa1220720:three_prime_UTR
AATGACAAGAAGACAAAGCT+TGG 0.556626 8_1:-84009718 None:intergenic
CTGATGATATATCACTAGCC+GGG 0.558208 8_1:+84010081 Msa1220720:CDS
TATCTGTTTGAGAGAGGCCA+TGG 0.565015 8_1:-84010497 None:intergenic
TGCAACTCCGAAGGCAGAGA+GGG 0.568528 8_1:+84010052 Msa1220720:CDS
TTTGATATGATGGTCGAGAT+CGG 0.574762 8_1:+84011184 Msa1220720:three_prime_UTR
TCCTAGAATTGCAATAAACA+TGG 0.588665 8_1:+84010773 Msa1220720:CDS
TGTGCAATATTGCAGGCAGG+GGG 0.610026 8_1:+84010354 Msa1220720:intron
AGATCGGTAACTGCGTCAAG+TGG 0.619691 8_1:+84011200 Msa1220720:three_prime_UTR
GATATATCACTAGCCGGGGA+CGG 0.624652 8_1:+84010086 Msa1220720:CDS
ATGGAAGAACAGTCTGAGAG+TGG 0.625981 8_1:-84009878 None:intergenic
CTGCGTCAAGTGGTTATGCA+GGG 0.634145 8_1:+84011210 Msa1220720:three_prime_UTR
CAACCATATCTGTTTGAGAG+AGG 0.635637 8_1:-84010503 None:intergenic
AGCTCAGCTCAACTCACTCG+TGG 0.647235 8_1:+84009766 Msa1220720:CDS
GCACTTAGGATCCCTATCAA+CGG 0.658126 8_1:-84010138 None:intergenic
TGATGATATATCACTAGCCG+GGG 0.662666 8_1:+84010082 Msa1220720:CDS
GAACCAATTGTTCAACATTG+GGG 0.665173 8_1:-84009807 None:intergenic
AGTGTTCAAAGAAATCTACC+TGG 0.677375 8_1:+84010432 Msa1220720:CDS
GAGGCCATGGAGATTAAACA+TGG 0.686465 8_1:-84010484 None:intergenic
GTGCAATATTGCAGGCAGGG+GGG 0.712500 8_1:+84010355 Msa1220720:intron
TTGCAACTCCGAAGGCAGAG+AGG 0.722926 8_1:+84010051 Msa1220720:CDS
TATTTCAAGAATCTACAACA+AGG 0.734171 8_1:+84010828 Msa1220720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAATTAAAAGGAAAA+AGG - chr8_1:84009949-84009968 None:intergenic 10.0%
!! TTAGAGTTTAATTAATTAAA+AGG - chr8_1:84009956-84009975 None:intergenic 10.0%
!!! TTAATAAAATTTGTTTTCAA+TGG + chr8_1:84011147-84011166 Msa1220720:three_prime_UTR 10.0%
!! TTAATTAATTAAAAGGAAAA+AGG - chr8_1:84009949-84009968 None:intergenic 10.0%
!! TTAGAGTTTAATTAATTAAA+AGG - chr8_1:84009956-84009975 None:intergenic 10.0%
!!! TTAATAAAATTTGTTTTCAA+TGG + chr8_1:84011147-84011166 Msa1220720:three_prime_UTR 10.0%
!!! TAAGATTAGTATTACTATTT+TGG - chr8_1:84010333-84010352 None:intergenic 15.0%
!!! AAGAAGTATAAACTATTATA+TGG - chr8_1:84010560-84010579 None:intergenic 15.0%
!! CATATTGTAAATTTAATGAT+GGG - chr8_1:84010716-84010735 None:intergenic 15.0%
!!! TAAGATTAGTATTACTATTT+TGG - chr8_1:84010333-84010352 None:intergenic 15.0%
!!! AAGAAGTATAAACTATTATA+TGG - chr8_1:84010560-84010579 None:intergenic 15.0%
!! CATATTGTAAATTTAATGAT+GGG - chr8_1:84010716-84010735 None:intergenic 15.0%
!!! AAACAAAAATTTGCTTTTAC+AGG + chr8_1:84010007-84010026 Msa1220720:intron 20.0%
!!! AACAAAAATTTGCTTTTACA+GGG + chr8_1:84010008-84010027 Msa1220720:intron 20.0%
!! ATATTTGTAATTCACTTACA+AGG + chr8_1:84010261-84010280 Msa1220720:intron 20.0%
!!! CCATTTTATAATGTTGAATT+GGG + chr8_1:84010378-84010397 Msa1220720:CDS 20.0%
!! GTATAAACTATTATATGGTA+GGG - chr8_1:84010555-84010574 None:intergenic 20.0%
!! AGTATAAACTATTATATGGT+AGG - chr8_1:84010556-84010575 None:intergenic 20.0%
!! CCTAAACATACATATTATAT+AGG - chr8_1:84010615-84010634 None:intergenic 20.0%
!! CCTATATAATATGTATGTTT+AGG + chr8_1:84010612-84010631 Msa1220720:intron 20.0%
!! GCATATTGTAAATTTAATGA+TGG - chr8_1:84010717-84010736 None:intergenic 20.0%
!! TATTAATGCTATTACTAAGT+TGG + chr8_1:84010953-84010972 Msa1220720:CDS 20.0%
!! ATTAATGCTATTACTAAGTT+GGG + chr8_1:84010954-84010973 Msa1220720:CDS 20.0%
!!! AAACAAAAATTTGCTTTTAC+AGG + chr8_1:84010007-84010026 Msa1220720:intron 20.0%
!!! AACAAAAATTTGCTTTTACA+GGG + chr8_1:84010008-84010027 Msa1220720:intron 20.0%
!! ATATTTGTAATTCACTTACA+AGG + chr8_1:84010261-84010280 Msa1220720:intron 20.0%
!!! CCATTTTATAATGTTGAATT+GGG + chr8_1:84010378-84010397 Msa1220720:CDS 20.0%
!! GTATAAACTATTATATGGTA+GGG - chr8_1:84010555-84010574 None:intergenic 20.0%
!! AGTATAAACTATTATATGGT+AGG - chr8_1:84010556-84010575 None:intergenic 20.0%
!! CCTAAACATACATATTATAT+AGG - chr8_1:84010615-84010634 None:intergenic 20.0%
!! CCTATATAATATGTATGTTT+AGG + chr8_1:84010612-84010631 Msa1220720:intron 20.0%
!! GCATATTGTAAATTTAATGA+TGG - chr8_1:84010717-84010736 None:intergenic 20.0%
!! TATTAATGCTATTACTAAGT+TGG + chr8_1:84010953-84010972 Msa1220720:CDS 20.0%
!! ATTAATGCTATTACTAAGTT+GGG + chr8_1:84010954-84010973 Msa1220720:CDS 20.0%
! AAAGAAAGTGATGAAATGAA+TGG - chr8_1:84010227-84010246 None:intergenic 25.0%
!!! GCCATTTTATAATGTTGAAT+TGG + chr8_1:84010377-84010396 Msa1220720:CDS 25.0%
! CCCAATTCAACATTATAAAA+TGG - chr8_1:84010381-84010400 None:intergenic 25.0%
! TTTATAATGTTGAATTGGGA+AGG + chr8_1:84010382-84010401 Msa1220720:CDS 25.0%
!!! TATTGATTGTCAAACTTTTG+AGG - chr8_1:84010810-84010829 None:intergenic 25.0%
! TATTTCAAGAATCTACAACA+AGG + chr8_1:84010828-84010847 Msa1220720:CDS 25.0%
! TAGAAAAAGTAGTACTGTTA+TGG + chr8_1:84011038-84011057 Msa1220720:exon 25.0%
! ATAAATGATCAAGTTCTTTG+AGG + chr8_1:84011307-84011326 Msa1220720:three_prime_UTR 25.0%
! AAAGAAAGTGATGAAATGAA+TGG - chr8_1:84010227-84010246 None:intergenic 25.0%
!!! GCCATTTTATAATGTTGAAT+TGG + chr8_1:84010377-84010396 Msa1220720:CDS 25.0%
! CCCAATTCAACATTATAAAA+TGG - chr8_1:84010381-84010400 None:intergenic 25.0%
! TTTATAATGTTGAATTGGGA+AGG + chr8_1:84010382-84010401 Msa1220720:CDS 25.0%
!!! TATTGATTGTCAAACTTTTG+AGG - chr8_1:84010810-84010829 None:intergenic 25.0%
! TATTTCAAGAATCTACAACA+AGG + chr8_1:84010828-84010847 Msa1220720:CDS 25.0%
! TAGAAAAAGTAGTACTGTTA+TGG + chr8_1:84011038-84011057 Msa1220720:exon 25.0%
! ATAAATGATCAAGTTCTTTG+AGG + chr8_1:84011307-84011326 Msa1220720:three_prime_UTR 25.0%
AACAGAACATTCATTAGCAA+TGG + chr8_1:84009690-84009709 Msa1220720:exon 30.0%
TTGTTGAAACTTCTGATTCA+CGG - chr8_1:84009839-84009858 None:intergenic 30.0%
ATCTTATTGTGCAATATTGC+AGG + chr8_1:84010347-84010366 Msa1220720:intron 30.0%
! GCTGATTCAAATTGAAATGA+GGG - chr8_1:84010458-84010477 None:intergenic 30.0%
! AGCTGATTCAAATTGAAATG+AGG - chr8_1:84010459-84010478 None:intergenic 30.0%
AATACCATGTTTAATCTCCA+TGG + chr8_1:84010480-84010499 Msa1220720:CDS 30.0%
TACAATATGCTTTCCAACTT+AGG + chr8_1:84010727-84010746 Msa1220720:CDS 30.0%
!! ATTCTAGGATCAACTCTTAA+AGG - chr8_1:84010762-84010781 None:intergenic 30.0%
TCCTAGAATTGCAATAAACA+TGG + chr8_1:84010773-84010792 Msa1220720:CDS 30.0%
TCCATGTTTATTGCAATTCT+AGG - chr8_1:84010777-84010796 None:intergenic 30.0%
AAGAATCTACAACAAGGAAA+AGG + chr8_1:84010834-84010853 Msa1220720:CDS 30.0%
!! AAGTGTTGTTTACTGATTCA+AGG + chr8_1:84010874-84010893 Msa1220720:CDS 30.0%
AAATTAGGTTTGATTGTACC+AGG + chr8_1:84011009-84011028 Msa1220720:CDS 30.0%
!!! CAGTACTACTTTTTCTAGTT+GGG - chr8_1:84011035-84011054 None:intergenic 30.0%
!!! ACAGTACTACTTTTTCTAGT+TGG - chr8_1:84011036-84011055 None:intergenic 30.0%
!!! CACCATTGATTTTGATATGA+TGG + chr8_1:84011174-84011193 Msa1220720:three_prime_UTR 30.0%
GACCATCATATCAAAATCAA+TGG - chr8_1:84011179-84011198 None:intergenic 30.0%
! TCCATCATCATCTTTTCTTT+TGG + chr8_1:84011241-84011260 Msa1220720:three_prime_UTR 30.0%
! CCATCATCATCTTTTCTTTT+GGG + chr8_1:84011242-84011261 Msa1220720:three_prime_UTR 30.0%
! CATCATCATCTTTTCTTTTG+GGG + chr8_1:84011243-84011262 Msa1220720:three_prime_UTR 30.0%
! ATCATCATCTTTTCTTTTGG+GGG + chr8_1:84011244-84011263 Msa1220720:three_prime_UTR 30.0%
AACAGAACATTCATTAGCAA+TGG + chr8_1:84009690-84009709 Msa1220720:exon 30.0%
TTGTTGAAACTTCTGATTCA+CGG - chr8_1:84009839-84009858 None:intergenic 30.0%
ATCTTATTGTGCAATATTGC+AGG + chr8_1:84010347-84010366 Msa1220720:intron 30.0%
! GCTGATTCAAATTGAAATGA+GGG - chr8_1:84010458-84010477 None:intergenic 30.0%
! AGCTGATTCAAATTGAAATG+AGG - chr8_1:84010459-84010478 None:intergenic 30.0%
AATACCATGTTTAATCTCCA+TGG + chr8_1:84010480-84010499 Msa1220720:CDS 30.0%
TACAATATGCTTTCCAACTT+AGG + chr8_1:84010727-84010746 Msa1220720:CDS 30.0%
!! ATTCTAGGATCAACTCTTAA+AGG - chr8_1:84010762-84010781 None:intergenic 30.0%
TCCTAGAATTGCAATAAACA+TGG + chr8_1:84010773-84010792 Msa1220720:CDS 30.0%
TCCATGTTTATTGCAATTCT+AGG - chr8_1:84010777-84010796 None:intergenic 30.0%
AAGAATCTACAACAAGGAAA+AGG + chr8_1:84010834-84010853 Msa1220720:CDS 30.0%
!! AAGTGTTGTTTACTGATTCA+AGG + chr8_1:84010874-84010893 Msa1220720:CDS 30.0%
AAATTAGGTTTGATTGTACC+AGG + chr8_1:84011009-84011028 Msa1220720:CDS 30.0%
!!! CAGTACTACTTTTTCTAGTT+GGG - chr8_1:84011035-84011054 None:intergenic 30.0%
!!! ACAGTACTACTTTTTCTAGT+TGG - chr8_1:84011036-84011055 None:intergenic 30.0%
!!! CACCATTGATTTTGATATGA+TGG + chr8_1:84011174-84011193 Msa1220720:three_prime_UTR 30.0%
GACCATCATATCAAAATCAA+TGG - chr8_1:84011179-84011198 None:intergenic 30.0%
! TCCATCATCATCTTTTCTTT+TGG + chr8_1:84011241-84011260 Msa1220720:three_prime_UTR 30.0%
! CCATCATCATCTTTTCTTTT+GGG + chr8_1:84011242-84011261 Msa1220720:three_prime_UTR 30.0%
! CATCATCATCTTTTCTTTTG+GGG + chr8_1:84011243-84011262 Msa1220720:three_prime_UTR 30.0%
! ATCATCATCTTTTCTTTTGG+GGG + chr8_1:84011244-84011263 Msa1220720:three_prime_UTR 30.0%
AATGACAAGAAGACAAAGCT+TGG - chr8_1:84009721-84009740 None:intergenic 35.0%
GAACCAATTGTTCAACATTG+GGG - chr8_1:84009810-84009829 None:intergenic 35.0%
CGAACCAATTGTTCAACATT+GGG - chr8_1:84009811-84009830 None:intergenic 35.0%
ACGAACCAATTGTTCAACAT+TGG - chr8_1:84009812-84009831 None:intergenic 35.0%
AACAAGAGATGTCGTCAATT+TGG + chr8_1:84010199-84010218 Msa1220720:CDS 35.0%
AGTGTTCAAAGAAATCTACC+TGG + chr8_1:84010432-84010451 Msa1220720:CDS 35.0%
ACAGATATGGTTGCATTATC+AGG + chr8_1:84010513-84010532 Msa1220720:CDS 35.0%
TGTTTAGGTGCACATACAAT+TGG + chr8_1:84010627-84010646 Msa1220720:intron 35.0%
CGTAGATTCTATTTGAGAAG+CGG - chr8_1:84010668-84010687 None:intergenic 35.0%
CGCTTCTCAAATAGAATCTA+CGG + chr8_1:84010666-84010685 Msa1220720:CDS 35.0%
ATGGGTCAATTCTACTTCTT+GGG - chr8_1:84010698-84010717 None:intergenic 35.0%
!! GTTTACTGATTCAAGGTCAA+AGG + chr8_1:84010881-84010900 Msa1220720:CDS 35.0%
!! AAAGCACTCTCAAAAGCTTT+TGG - chr8_1:84010933-84010952 None:intergenic 35.0%
! ATGCTATTACTAAGTTGGGA+AGG + chr8_1:84010958-84010977 Msa1220720:CDS 35.0%
! TGCTATTACTAAGTTGGGAA+GGG + chr8_1:84010959-84010978 Msa1220720:CDS 35.0%
GGTGTTAAAACTGGTAATCA+AGG + chr8_1:84010984-84011003 Msa1220720:CDS 35.0%
CTGGTAATCAAGGTGAAATT+AGG + chr8_1:84010994-84011013 Msa1220720:CDS 35.0%
!! ACCATTGATTTGAGACTGAA+CGG + chr8_1:84011111-84011130 Msa1220720:three_prime_UTR 35.0%
TTTGATATGATGGTCGAGAT+CGG + chr8_1:84011184-84011203 Msa1220720:three_prime_UTR 35.0%
CCCAAAAGAAAAGATGATGA+TGG - chr8_1:84011245-84011264 None:intergenic 35.0%
!! AGTTCTTTGAGGTATATTCC+TGG + chr8_1:84011318-84011337 Msa1220720:three_prime_UTR 35.0%
AATGACAAGAAGACAAAGCT+TGG - chr8_1:84009721-84009740 None:intergenic 35.0%
GAACCAATTGTTCAACATTG+GGG - chr8_1:84009810-84009829 None:intergenic 35.0%
CGAACCAATTGTTCAACATT+GGG - chr8_1:84009811-84009830 None:intergenic 35.0%
ACGAACCAATTGTTCAACAT+TGG - chr8_1:84009812-84009831 None:intergenic 35.0%
AACAAGAGATGTCGTCAATT+TGG + chr8_1:84010199-84010218 Msa1220720:CDS 35.0%
AGTGTTCAAAGAAATCTACC+TGG + chr8_1:84010432-84010451 Msa1220720:CDS 35.0%
ACAGATATGGTTGCATTATC+AGG + chr8_1:84010513-84010532 Msa1220720:CDS 35.0%
TGTTTAGGTGCACATACAAT+TGG + chr8_1:84010627-84010646 Msa1220720:intron 35.0%
CGTAGATTCTATTTGAGAAG+CGG - chr8_1:84010668-84010687 None:intergenic 35.0%
CGCTTCTCAAATAGAATCTA+CGG + chr8_1:84010666-84010685 Msa1220720:CDS 35.0%
ATGGGTCAATTCTACTTCTT+GGG - chr8_1:84010698-84010717 None:intergenic 35.0%
!! GTTTACTGATTCAAGGTCAA+AGG + chr8_1:84010881-84010900 Msa1220720:CDS 35.0%
!! AAAGCACTCTCAAAAGCTTT+TGG - chr8_1:84010933-84010952 None:intergenic 35.0%
! ATGCTATTACTAAGTTGGGA+AGG + chr8_1:84010958-84010977 Msa1220720:CDS 35.0%
! TGCTATTACTAAGTTGGGAA+GGG + chr8_1:84010959-84010978 Msa1220720:CDS 35.0%
GGTGTTAAAACTGGTAATCA+AGG + chr8_1:84010984-84011003 Msa1220720:CDS 35.0%
CTGGTAATCAAGGTGAAATT+AGG + chr8_1:84010994-84011013 Msa1220720:CDS 35.0%
!! ACCATTGATTTGAGACTGAA+CGG + chr8_1:84011111-84011130 Msa1220720:three_prime_UTR 35.0%
TTTGATATGATGGTCGAGAT+CGG + chr8_1:84011184-84011203 Msa1220720:three_prime_UTR 35.0%
CCCAAAAGAAAAGATGATGA+TGG - chr8_1:84011245-84011264 None:intergenic 35.0%
!! AGTTCTTTGAGGTATATTCC+TGG + chr8_1:84011318-84011337 Msa1220720:three_prime_UTR 35.0%
ATGCCCCAATGTTGAACAAT+TGG + chr8_1:84009804-84009823 Msa1220720:CDS 40.0%
TCTTCCATGATTGCTTTGTC+AGG + chr8_1:84009893-84009912 Msa1220720:CDS 40.0%
CTTCCATGATTGCTTTGTCA+GGG + chr8_1:84009894-84009913 Msa1220720:CDS 40.0%
ATACCCTGACAAAGCAATCA+TGG - chr8_1:84009900-84009919 None:intergenic 40.0%
CCTGATGATATATCACTAGC+CGG + chr8_1:84010080-84010099 Msa1220720:CDS 40.0%
CTGATGATATATCACTAGCC+GGG + chr8_1:84010081-84010100 Msa1220720:CDS 40.0%
TGATGATATATCACTAGCCG+GGG + chr8_1:84010082-84010101 Msa1220720:CDS 40.0%
! CAGCACAAGAGACTTTGTTT+CGG - chr8_1:84010163-84010182 None:intergenic 40.0%
TATTGTGCAATATTGCAGGC+AGG + chr8_1:84010351-84010370 Msa1220720:intron 40.0%
ATTGTGCAATATTGCAGGCA+GGG + chr8_1:84010352-84010371 Msa1220720:intron 40.0%
TTCAAATTGAAATGAGGGCC+AGG - chr8_1:84010453-84010472 None:intergenic 40.0%
CAACCATATCTGTTTGAGAG+AGG - chr8_1:84010506-84010525 None:intergenic 40.0%
GATGGGTCAATTCTACTTCT+TGG - chr8_1:84010699-84010718 None:intergenic 40.0%
AAGGACACATTTGCCTAAGT+TGG - chr8_1:84010743-84010762 None:intergenic 40.0%
!!! CAAACTTTTGAGGTGAGACA+GGG - chr8_1:84010800-84010819 None:intergenic 40.0%
!!! TCAAACTTTTGAGGTGAGAC+AGG - chr8_1:84010801-84010820 None:intergenic 40.0%
! ATTACTAAGTTGGGAAGGGT+TGG + chr8_1:84010963-84010982 Msa1220720:CDS 40.0%
TACTGTTATGGCATCATCGT+CGG + chr8_1:84011050-84011069 Msa1220720:three_prime_UTR 40.0%
! TTCGGAAGCATGCAGTTATA+TGG + chr8_1:84011074-84011093 Msa1220720:three_prime_UTR 40.0%
ACCGTTCAGTCTCAAATCAA+TGG - chr8_1:84011115-84011134 None:intergenic 40.0%
ATGCCCCAATGTTGAACAAT+TGG + chr8_1:84009804-84009823 Msa1220720:CDS 40.0%
TCTTCCATGATTGCTTTGTC+AGG + chr8_1:84009893-84009912 Msa1220720:CDS 40.0%
CTTCCATGATTGCTTTGTCA+GGG + chr8_1:84009894-84009913 Msa1220720:CDS 40.0%
ATACCCTGACAAAGCAATCA+TGG - chr8_1:84009900-84009919 None:intergenic 40.0%
CCTGATGATATATCACTAGC+CGG + chr8_1:84010080-84010099 Msa1220720:CDS 40.0%
CTGATGATATATCACTAGCC+GGG + chr8_1:84010081-84010100 Msa1220720:CDS 40.0%
TGATGATATATCACTAGCCG+GGG + chr8_1:84010082-84010101 Msa1220720:CDS 40.0%
! CAGCACAAGAGACTTTGTTT+CGG - chr8_1:84010163-84010182 None:intergenic 40.0%
TATTGTGCAATATTGCAGGC+AGG + chr8_1:84010351-84010370 Msa1220720:intron 40.0%
ATTGTGCAATATTGCAGGCA+GGG + chr8_1:84010352-84010371 Msa1220720:intron 40.0%
TTCAAATTGAAATGAGGGCC+AGG - chr8_1:84010453-84010472 None:intergenic 40.0%
CAACCATATCTGTTTGAGAG+AGG - chr8_1:84010506-84010525 None:intergenic 40.0%
GATGGGTCAATTCTACTTCT+TGG - chr8_1:84010699-84010718 None:intergenic 40.0%
AAGGACACATTTGCCTAAGT+TGG - chr8_1:84010743-84010762 None:intergenic 40.0%
!!! CAAACTTTTGAGGTGAGACA+GGG - chr8_1:84010800-84010819 None:intergenic 40.0%
!!! TCAAACTTTTGAGGTGAGAC+AGG - chr8_1:84010801-84010820 None:intergenic 40.0%
! ATTACTAAGTTGGGAAGGGT+TGG + chr8_1:84010963-84010982 Msa1220720:CDS 40.0%
TACTGTTATGGCATCATCGT+CGG + chr8_1:84011050-84011069 Msa1220720:three_prime_UTR 40.0%
! TTCGGAAGCATGCAGTTATA+TGG + chr8_1:84011074-84011093 Msa1220720:three_prime_UTR 40.0%
ACCGTTCAGTCTCAAATCAA+TGG - chr8_1:84011115-84011134 None:intergenic 40.0%
ATGGAAGAACAGTCTGAGAG+TGG - chr8_1:84009881-84009900 None:intergenic 45.0%
! GATTTTGCTTGCAACTCCGA+AGG + chr8_1:84010043-84010062 Msa1220720:CDS 45.0%
CCGGCTAGTGATATATCATC+AGG - chr8_1:84010083-84010102 None:intergenic 45.0%
!! CGGTTTTGACACTGTGGTTA+AGG + chr8_1:84010106-84010125 Msa1220720:CDS 45.0%
GCACTTAGGATCCCTATCAA+CGG - chr8_1:84010141-84010160 None:intergenic 45.0%
! GAGACTTTGTTTCGGCACTT+AGG - chr8_1:84010155-84010174 None:intergenic 45.0%
TTGTGCAATATTGCAGGCAG+GGG + chr8_1:84010353-84010372 Msa1220720:intron 45.0%
! TTGAATTGGGAAGGCGTGAT+GGG + chr8_1:84010391-84010410 Msa1220720:CDS 45.0%
GAGGCCATGGAGATTAAACA+TGG - chr8_1:84010487-84010506 None:intergenic 45.0%
TATCTGTTTGAGAGAGGCCA+TGG - chr8_1:84010500-84010519 None:intergenic 45.0%
TGGCCTCTCTCAAACAGATA+TGG + chr8_1:84010500-84010519 Msa1220720:CDS 45.0%
!! GGAAGGGTTGGTGTTAAAAC+TGG + chr8_1:84010975-84010994 Msa1220720:CDS 45.0%
!!! CTACTTTTTCTAGTTGGGCC+TGG - chr8_1:84011030-84011049 None:intergenic 45.0%
TATGGCATCATCGTCGGATT+CGG + chr8_1:84011056-84011075 Msa1220720:three_prime_UTR 45.0%
ATGGAAGAACAGTCTGAGAG+TGG - chr8_1:84009881-84009900 None:intergenic 45.0%
! GATTTTGCTTGCAACTCCGA+AGG + chr8_1:84010043-84010062 Msa1220720:CDS 45.0%
CCGGCTAGTGATATATCATC+AGG - chr8_1:84010083-84010102 None:intergenic 45.0%
!! CGGTTTTGACACTGTGGTTA+AGG + chr8_1:84010106-84010125 Msa1220720:CDS 45.0%
GCACTTAGGATCCCTATCAA+CGG - chr8_1:84010141-84010160 None:intergenic 45.0%
! GAGACTTTGTTTCGGCACTT+AGG - chr8_1:84010155-84010174 None:intergenic 45.0%
TTGTGCAATATTGCAGGCAG+GGG + chr8_1:84010353-84010372 Msa1220720:intron 45.0%
! TTGAATTGGGAAGGCGTGAT+GGG + chr8_1:84010391-84010410 Msa1220720:CDS 45.0%
GAGGCCATGGAGATTAAACA+TGG - chr8_1:84010487-84010506 None:intergenic 45.0%
TATCTGTTTGAGAGAGGCCA+TGG - chr8_1:84010500-84010519 None:intergenic 45.0%
TGGCCTCTCTCAAACAGATA+TGG + chr8_1:84010500-84010519 Msa1220720:CDS 45.0%
!! GGAAGGGTTGGTGTTAAAAC+TGG + chr8_1:84010975-84010994 Msa1220720:CDS 45.0%
!!! CTACTTTTTCTAGTTGGGCC+TGG - chr8_1:84011030-84011049 None:intergenic 45.0%
TATGGCATCATCGTCGGATT+CGG + chr8_1:84011056-84011075 Msa1220720:three_prime_UTR 45.0%
AAGAACAGTCTGAGAGTGGC+AGG - chr8_1:84009877-84009896 None:intergenic 50.0%
GATATATCACTAGCCGGGGA+CGG + chr8_1:84010086-84010105 Msa1220720:CDS 50.0%
TGACACTGTGGTTAAGGCCA+AGG + chr8_1:84010112-84010131 Msa1220720:CDS 50.0%
TGTGCAATATTGCAGGCAGG+GGG + chr8_1:84010354-84010373 Msa1220720:intron 50.0%
! GTTGAATTGGGAAGGCGTGA+TGG + chr8_1:84010390-84010409 Msa1220720:CDS 50.0%
AGATCGGTAACTGCGTCAAG+TGG + chr8_1:84011200-84011219 Msa1220720:three_prime_UTR 50.0%
ACTGCGTCAAGTGGTTATGC+AGG + chr8_1:84011209-84011228 Msa1220720:three_prime_UTR 50.0%
CTGCGTCAAGTGGTTATGCA+GGG + chr8_1:84011210-84011229 Msa1220720:three_prime_UTR 50.0%
AAGAACAGTCTGAGAGTGGC+AGG - chr8_1:84009877-84009896 None:intergenic 50.0%
GATATATCACTAGCCGGGGA+CGG + chr8_1:84010086-84010105 Msa1220720:CDS 50.0%
TGACACTGTGGTTAAGGCCA+AGG + chr8_1:84010112-84010131 Msa1220720:CDS 50.0%
TGTGCAATATTGCAGGCAGG+GGG + chr8_1:84010354-84010373 Msa1220720:intron 50.0%
! GTTGAATTGGGAAGGCGTGA+TGG + chr8_1:84010390-84010409 Msa1220720:CDS 50.0%
AGATCGGTAACTGCGTCAAG+TGG + chr8_1:84011200-84011219 Msa1220720:three_prime_UTR 50.0%
ACTGCGTCAAGTGGTTATGC+AGG + chr8_1:84011209-84011228 Msa1220720:three_prime_UTR 50.0%
CTGCGTCAAGTGGTTATGCA+GGG + chr8_1:84011210-84011229 Msa1220720:three_prime_UTR 50.0%
AGCTCAGCTCAACTCACTCG+TGG + chr8_1:84009766-84009785 Msa1220720:CDS 55.0%
TTGCAACTCCGAAGGCAGAG+AGG + chr8_1:84010051-84010070 Msa1220720:CDS 55.0%
TGCAACTCCGAAGGCAGAGA+GGG + chr8_1:84010052-84010071 Msa1220720:CDS 55.0%
GGATGTTCCCTCTCTGCCTT+CGG - chr8_1:84010062-84010081 None:intergenic 55.0%
CACAGTGTCAAAACCGTCCC+CGG - chr8_1:84010102-84010121 None:intergenic 55.0%
ATCCCTATCAACGGCTGCCT+TGG - chr8_1:84010132-84010151 None:intergenic 55.0%
GTGCAATATTGCAGGCAGGG+GGG + chr8_1:84010355-84010374 Msa1220720:intron 55.0%
AGCTCAGCTCAACTCACTCG+TGG + chr8_1:84009766-84009785 Msa1220720:CDS 55.0%
TTGCAACTCCGAAGGCAGAG+AGG + chr8_1:84010051-84010070 Msa1220720:CDS 55.0%
TGCAACTCCGAAGGCAGAGA+GGG + chr8_1:84010052-84010071 Msa1220720:CDS 55.0%
GGATGTTCCCTCTCTGCCTT+CGG - chr8_1:84010062-84010081 None:intergenic 55.0%
CACAGTGTCAAAACCGTCCC+CGG - chr8_1:84010102-84010121 None:intergenic 55.0%
ATCCCTATCAACGGCTGCCT+TGG - chr8_1:84010132-84010151 None:intergenic 55.0%
GTGCAATATTGCAGGCAGGG+GGG + chr8_1:84010355-84010374 Msa1220720:intron 55.0%
!! CGGGGACGGTTTTGACACTG+TGG + chr8_1:84010100-84010119 Msa1220720:CDS 60.0%
AGGCCAAGGCAGCCGTTGAT+AGG + chr8_1:84010126-84010145 Msa1220720:CDS 60.0%
GGCCAAGGCAGCCGTTGATA+GGG + chr8_1:84010127-84010146 Msa1220720:CDS 60.0%
!! CGGGGACGGTTTTGACACTG+TGG + chr8_1:84010100-84010119 Msa1220720:CDS 60.0%
AGGCCAAGGCAGCCGTTGAT+AGG + chr8_1:84010126-84010145 Msa1220720:CDS 60.0%
GGCCAAGGCAGCCGTTGATA+GGG + chr8_1:84010127-84010146 Msa1220720:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 84009686 84011345 84009686 ID=Msa1220720;Name=Msa1220720
chr8_1 mRNA 84009686 84011345 84009686 ID=Msa1220720-mRNA-1;Parent=Msa1220720;Name=Msa1220720-mRNA-1;_AED=0.06;_eAED=0.06;_QI=23|1|1|1|1|1|4|305|323
chr8_1 exon 84009686 84009915 84009686 ID=Msa1220720-mRNA-1:exon:30143;Parent=Msa1220720-mRNA-1
chr8_1 exon 84010029 84010220 84010029 ID=Msa1220720-mRNA-1:exon:30144;Parent=Msa1220720-mRNA-1
chr8_1 exon 84010369 84010534 84010369 ID=Msa1220720-mRNA-1:exon:30145;Parent=Msa1220720-mRNA-1
chr8_1 exon 84010634 84011345 84010634 ID=Msa1220720-mRNA-1:exon:30146;Parent=Msa1220720-mRNA-1
chr8_1 five_prime_UTR 84009686 84009708 84009686 ID=Msa1220720-mRNA-1:five_prime_utr;Parent=Msa1220720-mRNA-1
chr8_1 CDS 84009709 84009915 84009709 ID=Msa1220720-mRNA-1:cds;Parent=Msa1220720-mRNA-1
chr8_1 CDS 84010029 84010220 84010029 ID=Msa1220720-mRNA-1:cds;Parent=Msa1220720-mRNA-1
chr8_1 CDS 84010369 84010534 84010369 ID=Msa1220720-mRNA-1:cds;Parent=Msa1220720-mRNA-1
chr8_1 CDS 84010634 84011040 84010634 ID=Msa1220720-mRNA-1:cds;Parent=Msa1220720-mRNA-1
chr8_1 three_prime_UTR 84011041 84011345 84011041 ID=Msa1220720-mRNA-1:three_prime_utr;Parent=Msa1220720-mRNA-1
Gene Sequence

>Msa1220720

ATGGAAGCTCCAAGCTTTGTCTTCTTGTCATTGCTTCTTCTACTCACAGCTACAACAAGCTCAGCTCAACTCACTCGTGGCTTCTATAACAATGTATGCCCCAATGTTGAACAATTGGTTCGTTCTGCCGTGAATCAGAAGTTTCAACAAACATTTGTTACTGCTCCTGCCACTCTCAGACTGTTCTTCCATGATTGCTTTGTCAGGGGTTGTGATGCTTCGATTTTGCTTGCAACTCCGAAGGCAGAGAGGGAACATCCTGATGATATATCACTAGCCGGGGACGGTTTTGACACTGTGGTTAAGGCCAAGGCAGCCGTTGATAGGGATCCTAAGTGCCGAAACAAAGTCTCTTGTGCTGATATTCTTGCTCTCGCAACAAGAGATGTCGTCAATTTGGCAGGGGGGCCATTTTATAATGTTGAATTGGGAAGGCGTGATGGGAGAGTATCTACTATAGCAAGTGTTCAAAGAAATCTACCTGGCCCTCATTTCAATTTGAATCAGCTTAATACCATGTTTAATCTCCATGGCCTCTCTCAAACAGATATGGTTGCATTATCAGGTGCACATACAATTGGATTCTCTCATTGCAACCGCTTCTCAAATAGAATCTACGGATTCAGCCCAAGAAGTAGAATTGACCCATCATTAAATTTACAATATGCTTTCCAACTTAGGCAAATGTGTCCTTTAAGAGTTGATCCTAGAATTGCAATAAACATGGACCCTGTCTCACCTCAAAAGTTTGACAATCAATATTTCAAGAATCTACAACAAGGAAAAGGTCTATTCACTTCTGATCAAGTGTTGTTTACTGATTCAAGGTCAAAGGCTACAGTCAACTTATTTGCATCAAATCCAAAAGCTTTTGAGAGTGCTTTTATTAATGCTATTACTAAGTTGGGAAGGGTTGGTGTTAAAACTGGTAATCAAGGTGAAATTAGGTTTGATTGTACCAGGCCCAACTAG
Protein sequence

>Msa1220720

MEAPSFVFLSLLLLLTATTSSAQLTRGFYNNVCPNVEQLVRSAVNQKFQQTFVTAPATLRLFFHDCFVRGCDASILLATPKAEREHPDDISLAGDGFDTVVKAKAAVDRDPKCRNKVSCADILALATRDVVNLAGGPFYNVELGRRDGRVSTIASVQRNLPGPHFNLNQLNTMFNLHGLSQTDMVALSGAHTIGFSHCNRFSNRIYGFSPRSRIDPSLNLQYAFQLRQMCPLRVDPRIAINMDPVSPQKFDNQYFKNLQQGKGLFTSDQVLFTDSRSKATVNLFASNPKAFESAFINAITKLGRVGVKTGNQGEIRFDCTRPN*