AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1321550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1321550 MtrunA17_Chr8g0353331 97.674 172 4 0 1 172 1 172 6.05e-126 351
Msa1321550 MtrunA17_Chr7g0252391 55.952 168 71 2 1 165 1 168 1.59e-58 182
Msa1321550 MtrunA17_Chr3g0110281 52.500 160 65 3 4 160 35 186 9.06e-50 159
Msa1321550 MtrunA17_Chr5g0434521 47.134 157 74 2 5 160 14 162 4.06e-44 144
Msa1321550 MtrunA17_Chr6g0479111 47.134 157 74 2 1 157 73 220 1.20e-43 144
Msa1321550 MtrunA17_Chr1g0161131 47.468 158 75 3 1 157 1 151 2.90e-42 139
Msa1321550 MtrunA17_Chr8g0391621 48.428 159 72 5 1 157 1 151 7.25e-42 137
Msa1321550 MtrunA17_Chr1g0189471 87.879 66 8 0 1 66 1 66 2.30e-39 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1321550 AT3G12600.1 72.000 175 46 2 1 172 1 175 4.33e-81 238
Msa1321550 AT3G12600.2 70.732 164 45 2 12 172 3 166 3.62e-74 220
Msa1321550 AT3G26690.1 57.143 168 65 4 1 161 1 168 2.12e-54 171
Msa1321550 AT3G26690.2 57.143 168 65 4 1 161 1 168 2.12e-54 171
Msa1321550 AT1G12880.1 54.070 172 73 3 1 166 1 172 2.12e-52 166
Msa1321550 AT1G18300.1 48.428 159 75 2 3 160 42 194 8.05e-48 154
Msa1321550 AT1G14860.1 50.311 161 73 3 1 161 1 154 3.81e-46 149
Msa1321550 AT2G01670.1 50.955 157 70 3 1 157 6 155 9.97e-46 148
Msa1321550 AT1G73540.1 47.500 160 71 4 3 160 43 191 1.16e-43 144

Find 70 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAACAACGAAGACATTT+TGG 0.236717 8_4:-27579368 None:intergenic
GTTGCAGGTGTGTTCCCTTT+AGG 0.253270 8_4:+27581250 Msa1321550:intron
TTTGATCAAAACACAATTCT+TGG 0.263035 8_4:+27583472 Msa1321550:three_prime_UTR
ATTTCAATGGCGAAGGAAAT+TGG 0.279899 8_4:+27579449 Msa1321550:five_prime_UTR
TGTTTCTACTATGTCTGATT+TGG 0.325748 8_4:+27579542 Msa1321550:exon
GCAGCTTTACACAACCCTTC+TGG 0.339256 8_4:-27583191 None:intergenic
GTCTGATTTGGTTGCTCGTA+CGG 0.345456 8_4:+27579554 Msa1321550:CDS
GATGGTTATCGTCTTGTTGC+TGG 0.345818 8_4:+27579600 Msa1321550:CDS
ATCCAGCCAACTCCTATTTC+TGG 0.347905 8_4:-27583267 None:intergenic
TTGATCAAAACACAATTCTT+GGG 0.351474 8_4:+27583473 Msa1321550:three_prime_UTR
TCTATCTATAATTGATATAC+TGG 0.356719 8_4:+27583665 Msa1321550:three_prime_UTR
CTGAGCAAAATACCAGAAAT+AGG 0.370277 8_4:+27583255 Msa1321550:CDS
CTGGACTGAACTCATCTTGA+TGG 0.375486 8_4:-27583172 None:intergenic
TCAAGGAGGAACTTGAACTA+TGG 0.378915 8_4:+27583231 Msa1321550:CDS
TTTGCTGACAATTTATTTGT+AGG 0.379791 8_4:+27583439 Msa1321550:three_prime_UTR
TTGAAATCGAAATTACAATA+AGG 0.381048 8_4:-27579433 None:intergenic
CTCTGGAACGCTTCTGCAAA+TGG 0.393459 8_4:+27583336 Msa1321550:CDS
TATTGTAATTTCGATTTCAA+TGG 0.396745 8_4:+27579436 Msa1321550:five_prime_UTR
TATATCAATTATAGATAGAT+AGG 0.397854 8_4:-27583661 None:intergenic
GTCTTGGATACTATGAGTTT+AGG 0.399550 8_4:+27583141 Msa1321550:CDS
TTGCTGACAATTTATTTGTA+GGG 0.401875 8_4:+27583440 Msa1321550:three_prime_UTR
TCTGATTTGGTTGCTCGTAC+GGG 0.430251 8_4:+27579555 Msa1321550:CDS
AAGCACTTCAACAATCTTCT+CGG 0.437562 8_4:-27581301 None:intergenic
ATAGAAGAAGCGGGAGTTCG+AGG 0.477400 8_4:+27582535 Msa1321550:CDS
AAGCGGTTAGAACGGATCAA+TGG 0.477919 8_4:-27579254 None:intergenic
TGAGTGGATGAGGGACGCTC+TGG 0.483748 8_4:+27583319 Msa1321550:CDS
ATTCTTGGGGTAGTTACTCT+AGG 0.495566 8_4:+27583487 Msa1321550:three_prime_UTR
GCCTCCGACATGAGTGGATG+AGG 0.497019 8_4:+27583309 Msa1321550:CDS
GGGAGTTCGAGGAGACCTAA+TGG 0.502057 8_4:+27582546 Msa1321550:CDS
TACCAGAAATAGGAGTTGGC+TGG 0.502905 8_4:+27583265 Msa1321550:CDS
AAAATACCAGAAATAGGAGT+TGG 0.506282 8_4:+27583261 Msa1321550:CDS
TAGGAGTTGGCTGGATGTAT+CGG 0.513352 8_4:+27583274 Msa1321550:CDS
ATGGTTATCGTCTTGTTGCT+GGG 0.519282 8_4:+27579601 Msa1321550:CDS
AGCGGTTAGAACGGATCAAT+GGG 0.520314 8_4:-27579253 None:intergenic
TGGACTGAACTCATCTTGAT+GGG 0.521294 8_4:-27583171 None:intergenic
GGGAAAAGGAGAGAACGAAA+AGG 0.522409 8_4:-27579279 None:intergenic
CAGCAAATCAGTCTAACATC+AGG 0.526302 8_4:-27583396 None:intergenic
TCTGCGGAATGATGTCTGTC+CGG 0.527991 8_4:+27583549 Msa1321550:three_prime_UTR
AATGTTTGCTTTGTTTGTCA+AGG 0.532894 8_4:+27583214 Msa1321550:CDS
CATCAGCAACGCTATGAAGA+TGG 0.535027 8_4:+27579582 Msa1321550:CDS
TGCTGACAATTTATTTGTAG+GGG 0.539264 8_4:+27583441 Msa1321550:three_prime_UTR
CAGTTATTGTTTCAAGTCTG+CGG 0.546544 8_4:+27583533 Msa1321550:three_prime_UTR
AAAAGGAGAAGCGGTTAGAA+CGG 0.546783 8_4:-27579262 None:intergenic
ATGGCATGAGGAAAAGTTTG+TGG 0.551089 8_4:+27583355 Msa1321550:CDS
CAAGATGAGTTCAGTCCAGA+AGG 0.552506 8_4:+27583176 Msa1321550:CDS
ATTTGGTTGCTCGTACGGGT+CGG 0.565256 8_4:+27579559 Msa1321550:CDS
AGAGAAGCTATAGAAGAAGC+GGG 0.567209 8_4:+27582526 Msa1321550:CDS
AAGAGAAGCTATAGAAGAAG+CGG 0.568408 8_4:+27582525 Msa1321550:CDS
AACAAAAGACCTGGTCCACT+AGG 0.578546 8_4:-27581336 None:intergenic
TGATGAAACTGTTGAGGAGG+CGG 0.578996 8_4:+27582498 Msa1321550:CDS
ATTAATTCACCTAGTGGACC+AGG 0.583292 8_4:+27581327 Msa1321550:CDS
GTTTGCTTTGTTTGTCAAGG+AGG 0.584229 8_4:+27583217 Msa1321550:CDS
GCTGACAATTTATTTGTAGG+GGG 0.592512 8_4:+27583442 Msa1321550:three_prime_UTR
TGATCAAAACACAATTCTTG+GGG 0.601836 8_4:+27583474 Msa1321550:three_prime_UTR
CTTATGATTAATTCACCTAG+TGG 0.602210 8_4:+27581321 Msa1321550:CDS
ACCTTTGGAAACAAAAGACC+TGG 0.602229 8_4:-27581345 None:intergenic
CCCTCATCCACTCATGTCGG+AGG 0.604589 8_4:-27583310 None:intergenic
GAATGATGAAACTGTTGAGG+AGG 0.612719 8_4:+27582495 Msa1321550:CDS
AATTTCGATTTCAATGGCGA+AGG 0.612949 8_4:+27579442 Msa1321550:five_prime_UTR
TTGATAGCCTCCGACATGAG+TGG 0.618790 8_4:+27583303 Msa1321550:CDS
CATGAGGAAAAGTTTGTGGA+TGG 0.623665 8_4:+27583359 Msa1321550:CDS
ACGCTTCTGCAAATGGCATG+AGG 0.629815 8_4:+27583343 Msa1321550:CDS
CGTCCCTCATCCACTCATGT+CGG 0.636052 8_4:-27583313 None:intergenic
GAGAGAACGAAAAGGAGAAG+CGG 0.638095 8_4:-27579271 None:intergenic
CCTCCGACATGAGTGGATGA+GGG 0.641158 8_4:+27583310 Msa1321550:CDS
GTTACTCTGAATCAAAAGAC+CGG 0.645377 8_4:-27583568 None:intergenic
GAGTTGGCTGGATGTATCGG+AGG 0.659992 8_4:+27583277 Msa1321550:CDS
AAGATGAGTTCAGTCCAGAA+GGG 0.667305 8_4:+27583177 Msa1321550:CDS
CAATTATAGATAGATAGGTG+TGG 0.670020 8_4:-27583656 None:intergenic
GGAGAATGATGAAACTGTTG+AGG 0.693121 8_4:+27582492 Msa1321550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAATAAAAATAAAAAA+GGG - chr8_4:27579640-27579659 None:intergenic 0.0%
!! TAATTAAATAAAAATAAAAA+AGG - chr8_4:27579641-27579660 None:intergenic 0.0%
!! TTAAATAAAAATAAAAAAGG+GGG - chr8_4:27579638-27579657 None:intergenic 10.0%
!!! ATTAATAATTATTGTTTTAG+GGG + chr8_4:27579674-27579693 Msa1321550:intron 10.0%
!!! AATATGTATGTATATTTTTA+TGG + chr8_4:27580242-27580261 Msa1321550:intron 10.0%
!!! TTTATAAAGAAGTATTATAA+AGG + chr8_4:27580368-27580387 Msa1321550:intron 10.0%
!!! TTGAGAATTTTTTTTGAATT+TGG + chr8_4:27579486-27579505 Msa1321550:five_prime_UTR 15.0%
!! TTGGATTTGATTAATAAAAA+AGG + chr8_4:27579505-27579524 Msa1321550:five_prime_UTR 15.0%
!! TGGATTTGATTAATAAAAAA+GGG + chr8_4:27579506-27579525 Msa1321550:five_prime_UTR 15.0%
!! TATATGCAATGATTAATATT+TGG + chr8_4:27580132-27580151 Msa1321550:intron 15.0%
!! AATTATGAAATGAATTTGAT+TGG + chr8_4:27581057-27581076 Msa1321550:intron 15.0%
!!! TAAAAGTTGATTAATGAAAA+AGG - chr8_4:27581122-27581141 None:intergenic 15.0%
!!! TGATAAAAATATTTTCAGAT+AGG + chr8_4:27581145-27581164 Msa1321550:intron 15.0%
!!! AAAAATTTCACTTATTTCTT+TGG - chr8_4:27581668-27581687 None:intergenic 15.0%
!!! AATAAGTGAAATTTTTCTTA+TGG + chr8_4:27581672-27581691 Msa1321550:intron 15.0%
!!! ATACATATATATTTTGAAGA+AGG + chr8_4:27581892-27581911 Msa1321550:intron 15.0%
!!! TACATATATATTTTGAAGAA+GGG + chr8_4:27581893-27581912 Msa1321550:intron 15.0%
!!! ACATTTAAAATATGATGAAT+TGG + chr8_4:27582810-27582829 Msa1321550:intron 15.0%
!!! TGTTATTTTAATTGTAAACA+AGG + chr8_4:27583028-27583047 Msa1321550:intron 15.0%
!! TATATCAATTATAGATAGAT+AGG - chr8_4:27583664-27583683 None:intergenic 15.0%
!! TTGAAATCGAAATTACAATA+AGG - chr8_4:27579436-27579455 None:intergenic 20.0%
!! TATTGTAATTTCGATTTCAA+TGG + chr8_4:27579436-27579455 Msa1321550:five_prime_UTR 20.0%
!!! GAAAAGAATGTTTTTTGTTT+TGG + chr8_4:27579717-27579736 Msa1321550:intron 20.0%
!!! TTTATTTGTGTGTTTGTTTT+TGG + chr8_4:27579787-27579806 Msa1321550:intron 20.0%
!!! TCTCTATTATGGTTTTTTAA+GGG + chr8_4:27579844-27579863 Msa1321550:intron 20.0%
!!! TTAATTTGATGATATACGAA+TGG + chr8_4:27579913-27579932 Msa1321550:intron 20.0%
!! AATGGCTGAAATTAAATTTA+AGG + chr8_4:27579931-27579950 Msa1321550:intron 20.0%
!! AAAAGAAGAAGAAAAAAACA+TGG - chr8_4:27580442-27580461 None:intergenic 20.0%
!! TGCTAAATTGATTAAAACAA+TGG + chr8_4:27580509-27580528 Msa1321550:intron 20.0%
!! GTTCTTAGAAAACTAAAAAT+AGG - chr8_4:27580625-27580644 None:intergenic 20.0%
!! GGAAAATAATAAACAAAGAT+AGG - chr8_4:27580810-27580829 None:intergenic 20.0%
!!! TAAATGTAATTTTAATCCTC+TGG + chr8_4:27580886-27580905 Msa1321550:intron 20.0%
!!! TTTTTCTTATATACCTGTTT+TGG + chr8_4:27581186-27581205 Msa1321550:intron 20.0%
!! TATTTCAAACATTATACCTT+TGG - chr8_4:27581363-27581382 None:intergenic 20.0%
!!! TTCTTTTCAGTCATTATATT+GGG + chr8_4:27581484-27581503 Msa1321550:intron 20.0%
!! ACCTCTTATACTAATTATAA+TGG + chr8_4:27581538-27581557 Msa1321550:intron 20.0%
!! ACCATTATAATTAGTATAAG+AGG - chr8_4:27581542-27581561 None:intergenic 20.0%
!!! TCTTTCTAATTAAAAGTATC+AGG - chr8_4:27581699-27581718 None:intergenic 20.0%
!!! TTGTACTAAAATTCTTTTCT+TGG + chr8_4:27581744-27581763 Msa1321550:intron 20.0%
!! TTCTCTAATATCTACTATTA+TGG - chr8_4:27582104-27582123 None:intergenic 20.0%
!!! TTTTAAACAGTAACTTAATC+TGG + chr8_4:27582377-27582396 Msa1321550:intron 20.0%
!!! ATATTTTGATGATCTCATAA+TGG + chr8_4:27582655-27582674 Msa1321550:intron 20.0%
!!! TATTTTGATGATCTCATAAT+GGG + chr8_4:27582656-27582675 Msa1321550:intron 20.0%
!! TCTATCTATAATTGATATAC+TGG + chr8_4:27583665-27583684 Msa1321550:three_prime_UTR 20.0%
!!! GTCTCTATTATGGTTTTTTA+AGG + chr8_4:27579843-27579862 Msa1321550:intron 25.0%
!!! CTCTATTATGGTTTTTTAAG+GGG + chr8_4:27579845-27579864 Msa1321550:intron 25.0%
! CATATGAAAGTCAATGTATA+AGG + chr8_4:27579870-27579889 Msa1321550:intron 25.0%
!!! ATTTTTATGGTCAACTTGTT+AGG + chr8_4:27580255-27580274 Msa1321550:intron 25.0%
!!! TTTTTATGGTCAACTTGTTA+GGG + chr8_4:27580256-27580275 Msa1321550:intron 25.0%
!! CATTTTCACAAAATAGGATT+AGG + chr8_4:27580280-27580299 Msa1321550:intron 25.0%
! AAAATAGGATTAGGATTACT+TGG + chr8_4:27580289-27580308 Msa1321550:intron 25.0%
!! TAATTAGTCTATTTAGCTAC+AGG - chr8_4:27580332-27580351 None:intergenic 25.0%
! AGCTATAAAATACAACTCTA+AGG - chr8_4:27580662-27580681 None:intergenic 25.0%
! TAAGACAGATACTAAACAAT+TGG - chr8_4:27580983-27581002 None:intergenic 25.0%
! AATACTAAAAAGTAAACGAC+TGG + chr8_4:27581018-27581037 Msa1321550:intron 25.0%
! AAATCACTCTAACTACAAAT+GGG + chr8_4:27581086-27581105 Msa1321550:intron 25.0%
!! GTTCTTTTCAGTCATTATAT+TGG + chr8_4:27581483-27581502 Msa1321550:intron 25.0%
! AGTCATTATATTGGGAAATT+TGG + chr8_4:27581492-27581511 Msa1321550:intron 25.0%
! TTGAAAACTAGTTAGGTAAA+TGG + chr8_4:27581995-27582014 Msa1321550:intron 25.0%
! GTTTAGGTAATAAAATAGCT+TGG + chr8_4:27582236-27582255 Msa1321550:intron 25.0%
! ATGAGCATTTCCTAAATTAT+TGG - chr8_4:27582285-27582304 None:intergenic 25.0%
! AATTCATATTTCTCACCATT+AGG - chr8_4:27582564-27582583 None:intergenic 25.0%
! TAGCTTCATTAATATCATGA+TGG + chr8_4:27582618-27582637 Msa1321550:intron 25.0%
! AGCTTCATTAATATCATGAT+GGG + chr8_4:27582619-27582638 Msa1321550:intron 25.0%
!! AAGTTTTCTTAACTTTGCAT+GGG + chr8_4:27582762-27582781 Msa1321550:intron 25.0%
! TAATATTCCACCCTATTATA+GGG - chr8_4:27582837-27582856 None:intergenic 25.0%
! ATAATATTCCACCCTATTAT+AGG - chr8_4:27582838-27582857 None:intergenic 25.0%
!! TTCTACTTTCTACATGATTT+TGG + chr8_4:27582915-27582934 Msa1321550:intron 25.0%
! TTTGCTGACAATTTATTTGT+AGG + chr8_4:27583439-27583458 Msa1321550:three_prime_UTR 25.0%
! TTGCTGACAATTTATTTGTA+GGG + chr8_4:27583440-27583459 Msa1321550:three_prime_UTR 25.0%
! TTTGATCAAAACACAATTCT+TGG + chr8_4:27583472-27583491 Msa1321550:three_prime_UTR 25.0%
! TTGATCAAAACACAATTCTT+GGG + chr8_4:27583473-27583492 Msa1321550:three_prime_UTR 25.0%
AAGAATGAAGAAATGGGAAA+AGG - chr8_4:27579296-27579315 None:intergenic 30.0%
AGAGAGAAGAATGAAGAAAT+GGG - chr8_4:27579302-27579321 None:intergenic 30.0%
!!! CGAAGACATTTTGGTTTTTA+GGG - chr8_4:27579362-27579381 None:intergenic 30.0%
!!! ACGAAGACATTTTGGTTTTT+AGG - chr8_4:27579363-27579382 None:intergenic 30.0%
! TGTTTCTACTATGTCTGATT+TGG + chr8_4:27579542-27579561 Msa1321550:exon 30.0%
!!! GGAAACATGTCATTTTCTTA+CGG + chr8_4:27579695-27579714 Msa1321550:intron 30.0%
GTGTTTGAGAAATTACGATT+TGG + chr8_4:27579739-27579758 Msa1321550:intron 30.0%
!!! TTACGATTTGGTTTTGTTGA+TGG + chr8_4:27579751-27579770 Msa1321550:intron 30.0%
!!! TTTTGTTGATGGTGATGTTT+TGG + chr8_4:27579762-27579781 Msa1321550:intron 30.0%
GTTGATGTATGTCTCTATTA+TGG + chr8_4:27579833-27579852 Msa1321550:intron 30.0%
AAAGTCAATGTATAAGGTGA+AGG + chr8_4:27579876-27579895 Msa1321550:intron 30.0%
TGATTATATGCTATGAAGCA+CGG + chr8_4:27579973-27579992 Msa1321550:intron 30.0%
AATGATGTCAGAAAATGACA+TGG + chr8_4:27580030-27580049 Msa1321550:intron 30.0%
! ATGTAATGACTTGTGATGTT+AGG + chr8_4:27580057-27580076 Msa1321550:intron 30.0%
!! ACCTTTTTGATTAGAAGTGT+CGG + chr8_4:27580097-27580116 Msa1321550:intron 30.0%
!!! GGTCTTGCTTTTTATGTTTT+AGG + chr8_4:27580153-27580172 Msa1321550:intron 30.0%
GATAATACTTGTGTTGCATA+TGG + chr8_4:27580217-27580236 Msa1321550:intron 30.0%
!!! TTTTATGGTCAACTTGTTAG+GGG + chr8_4:27580257-27580276 Msa1321550:intron 30.0%
! CATGCATATTTGATGATTGA+TGG + chr8_4:27580413-27580432 Msa1321550:intron 30.0%
TAGATTGTATCTGTTGTGAT+AGG + chr8_4:27580483-27580502 Msa1321550:intron 30.0%
ATTAAAACAATGGATGAGTC+TGG + chr8_4:27580519-27580538 Msa1321550:intron 30.0%
TTATGCATTATGACAACGTT+TGG + chr8_4:27580681-27580700 Msa1321550:intron 30.0%
TACATGTATCAGTAACATGT+AGG - chr8_4:27580759-27580778 None:intergenic 30.0%
GAATGAAACTATTACATGCT+GGG - chr8_4:27580831-27580850 None:intergenic 30.0%
AGAATGAAACTATTACATGC+TGG - chr8_4:27580832-27580851 None:intergenic 30.0%
GAAATCACTCTAACTACAAA+TGG + chr8_4:27581085-27581104 Msa1321550:intron 30.0%
TCTTCAAAACAGAACTTGAA+GGG - chr8_4:27581220-27581239 None:intergenic 30.0%
ATCTTCAAAACAGAACTTGA+AGG - chr8_4:27581221-27581240 None:intergenic 30.0%
!!! TCTGTTTTGAAGATGTCAAT+TGG + chr8_4:27581227-27581246 Msa1321550:intron 30.0%
!!! CTGTTTTGAAGATGTCAATT+GGG + chr8_4:27581228-27581247 Msa1321550:intron 30.0%
! TCACAGCTTTTATACCTAAA+GGG - chr8_4:27581267-27581286 None:intergenic 30.0%
! TTCACAGCTTTTATACCTAA+AGG - chr8_4:27581268-27581287 None:intergenic 30.0%
CTTATGATTAATTCACCTAG+TGG + chr8_4:27581321-27581340 Msa1321550:CDS 30.0%
ACAGAACTGCTTTGTAAATT+TGG + chr8_4:27581588-27581607 Msa1321550:intron 30.0%
AACTGCTTTGTAAATTTGGA+CGG + chr8_4:27581592-27581611 Msa1321550:intron 30.0%
!!! AATTGGTTTTACTATCCCTA+TGG + chr8_4:27581616-27581635 Msa1321550:intron 30.0%
AAGAGAATATCATGTGCATT+AGG + chr8_4:27581715-27581734 Msa1321550:intron 30.0%
TTTGTTTCCAAAGATGACTT+AGG + chr8_4:27581837-27581856 Msa1321550:intron 30.0%
TAAAATACCTTCATGCTAGT+TGG + chr8_4:27581939-27581958 Msa1321550:intron 30.0%
AATTAGGTTCGACTATATCA+AGG - chr8_4:27582199-27582218 None:intergenic 30.0%
!! GTTTTGAGCTCCAATAATTT+AGG + chr8_4:27582272-27582291 Msa1321550:intron 30.0%
!!! GGTTTGCGTTGTTTTTTTAA+TGG + chr8_4:27582409-27582428 Msa1321550:intron 30.0%
!!! TTTTTTTAATGGACCCTAAG+TGG + chr8_4:27582420-27582439 Msa1321550:intron 30.0%
!!! TTGCGTTGTTTTTCCATTAA+AGG + chr8_4:27582462-27582481 Msa1321550:intron 30.0%
!!! TGCGTTGTTTTTCCATTAAA+GGG + chr8_4:27582463-27582482 Msa1321550:intron 30.0%
TATTCTTTGTTGCTGTAAGT+TGG + chr8_4:27582728-27582747 Msa1321550:intron 30.0%
! GAAGTTTTCTTAACTTTGCA+TGG + chr8_4:27582761-27582780 Msa1321550:intron 30.0%
ATGAATTGGACCCTATAATA+GGG + chr8_4:27582824-27582843 Msa1321550:intron 30.0%
!! CTTTCAAGTTACAACAGTAA+AGG - chr8_4:27582981-27583000 None:intergenic 30.0%
!!! TATCGATCTTGTCATTTTGA+TGG + chr8_4:27583075-27583094 Msa1321550:intron 30.0%
!! AATGTTTGCTTTGTTTGTCA+AGG + chr8_4:27583214-27583233 Msa1321550:CDS 30.0%
AAAATACCAGAAATAGGAGT+TGG + chr8_4:27583261-27583280 Msa1321550:CDS 30.0%
TGCTGACAATTTATTTGTAG+GGG + chr8_4:27583441-27583460 Msa1321550:three_prime_UTR 30.0%
TGATCAAAACACAATTCTTG+GGG + chr8_4:27583474-27583493 Msa1321550:three_prime_UTR 30.0%
CAATTATAGATAGATAGGTG+TGG - chr8_4:27583659-27583678 None:intergenic 30.0%
GAGAGAGAAGAATGAAGAAA+TGG - chr8_4:27579303-27579322 None:intergenic 35.0%
!!! GTTTTTAGGGTTTGCATAAG+AGG - chr8_4:27579349-27579368 None:intergenic 35.0%
! TCTCAACAACGAAGACATTT+TGG - chr8_4:27579371-27579390 None:intergenic 35.0%
AATTTCGATTTCAATGGCGA+AGG + chr8_4:27579442-27579461 Msa1321550:five_prime_UTR 35.0%
ATTTCAATGGCGAAGGAAAT+TGG + chr8_4:27579449-27579468 Msa1321550:five_prime_UTR 35.0%
!!! TGTGTGTTTGTTTTTGGTTG+TGG + chr8_4:27579793-27579812 Msa1321550:intron 35.0%
!!! TTGTTTTTGGTTGTGGAAGA+TGG + chr8_4:27579800-27579819 Msa1321550:intron 35.0%
ACCGACACTTCTAATCAAAA+AGG - chr8_4:27580101-27580120 None:intergenic 35.0%
!!! TAGGGGCATTTTCACAAAAT+AGG + chr8_4:27580274-27580293 Msa1321550:intron 35.0%
!! GACTAATTAATGAAGCACTG+AGG + chr8_4:27580343-27580362 Msa1321550:intron 35.0%
AAGTTCAGTTGAATCGAATG+TGG + chr8_4:27580705-27580724 Msa1321550:intron 35.0%
TGTAGTCCTGGAAATATCAA+TGG + chr8_4:27580775-27580794 Msa1321550:intron 35.0%
CTTATGCCATTGATATTTCC+AGG - chr8_4:27580784-27580803 None:intergenic 35.0%
CAAGATAAGTCAAAAACCAG+AGG - chr8_4:27580905-27580924 None:intergenic 35.0%
!!! TGGTTTTTGACTTATCTTGC+TGG + chr8_4:27580906-27580925 Msa1321550:intron 35.0%
!! AAGGGTATCATATCCAAAAC+AGG - chr8_4:27581202-27581221 None:intergenic 35.0%
AAGCACTTCAACAATCTTCT+CGG - chr8_4:27581304-27581323 None:intergenic 35.0%
! AACCTGTTTGAGTATTGTTG+TGG + chr8_4:27581393-27581412 Msa1321550:intron 35.0%
AACCACAACAATACTCAAAC+AGG - chr8_4:27581398-27581417 None:intergenic 35.0%
! GTTGTGGTTGATACTAGTTT+AGG + chr8_4:27581409-27581428 Msa1321550:intron 35.0%
TTCTGTTGTAATGATGGCTA+CGG - chr8_4:27581574-27581593 None:intergenic 35.0%
AAGCAGTTCTGTTGTAATGA+TGG - chr8_4:27581580-27581599 None:intergenic 35.0%
ACTGCTTTGTAAATTTGGAC+GGG + chr8_4:27581593-27581612 Msa1321550:intron 35.0%
TTGTAAATTTGGACGGGAAT+TGG + chr8_4:27581599-27581618 Msa1321550:intron 35.0%
TTTCTTGGAATTGACTGATG+TGG + chr8_4:27581759-27581778 Msa1321550:intron 35.0%
ATGTGGACGATAAAGGATTA+TGG + chr8_4:27581776-27581795 Msa1321550:intron 35.0%
CTCTAATCCTAAGTCATCTT+TGG - chr8_4:27581847-27581866 None:intergenic 35.0%
GATAGTCACAAACTGAACAT+AGG + chr8_4:27581968-27581987 Msa1321550:intron 35.0%
AGGACTCTTGAAAACTAGTT+AGG + chr8_4:27581988-27582007 Msa1321550:intron 35.0%
AACAAGCTCAAGCTTGAATT+AGG - chr8_4:27582215-27582234 None:intergenic 35.0%
! AAGCTTGAGCTTGTTTGTTT+AGG + chr8_4:27582220-27582239 Msa1321550:intron 35.0%
AACTTAATCTGGTTGAGATC+AGG + chr8_4:27582388-27582407 Msa1321550:intron 35.0%
!!! GTTGTTTTTCCATTAAAGGG+AGG + chr8_4:27582466-27582485 Msa1321550:intron 35.0%
! TTTTTCCATTAAAGGGAGGT+TGG + chr8_4:27582470-27582489 Msa1321550:intron 35.0%
! TTTTCCATTAAAGGGAGGTT+GGG + chr8_4:27582471-27582490 Msa1321550:intron 35.0%
AAGAGAAGCTATAGAAGAAG+CGG + chr8_4:27582525-27582544 Msa1321550:CDS 35.0%
!! ACTTTAGAACTTCTGCAATG+TGG + chr8_4:27582589-27582608 Msa1321550:intron 35.0%
GATGAATTGGACCCTATAAT+AGG + chr8_4:27582823-27582842 Msa1321550:intron 35.0%
! CTCACATCTGAAATAAATGC+TGG - chr8_4:27582882-27582901 None:intergenic 35.0%
AACAAAAAGGTTTCAGCCAT+CGG - chr8_4:27583112-27583131 None:intergenic 35.0%
AGACATTCCTGACAACAAAA+AGG - chr8_4:27583125-27583144 None:intergenic 35.0%
!! TTTTGTTGTCAGGAATGTCT+TGG + chr8_4:27583125-27583144 Msa1321550:intron 35.0%
GTCTTGGATACTATGAGTTT+AGG + chr8_4:27583141-27583160 Msa1321550:CDS 35.0%
!!! CTATTTCTGGTATTTTGCTC+AGG - chr8_4:27583257-27583276 None:intergenic 35.0%
CTGAGCAAAATACCAGAAAT+AGG + chr8_4:27583255-27583274 Msa1321550:CDS 35.0%
GCTGACAATTTATTTGTAGG+GGG + chr8_4:27583442-27583461 Msa1321550:three_prime_UTR 35.0%
! CAGTTATTGTTTCAAGTCTG+CGG + chr8_4:27583533-27583552 Msa1321550:three_prime_UTR 35.0%
GTTACTCTGAATCAAAAGAC+CGG - chr8_4:27583571-27583590 None:intergenic 35.0%
AAAAGGAGAAGCGGTTAGAA+CGG - chr8_4:27579265-27579284 None:intergenic 40.0%
! ATGGTTATCGTCTTGTTGCT+GGG + chr8_4:27579601-27579620 Msa1321550:CDS 40.0%
GTCAATGTATAAGGTGAAGG+TGG + chr8_4:27579879-27579898 Msa1321550:intron 40.0%
TGATGTTAGGAGTCAGATAC+TGG + chr8_4:27580070-27580089 Msa1321550:intron 40.0%
AACAATGGATGAGTCTGGAT+TGG + chr8_4:27580524-27580543 Msa1321550:intron 40.0%
GTTACTGATACATGTAGTCC+TGG + chr8_4:27580763-27580782 Msa1321550:intron 40.0%
! ATTAATTCACCTAGTGGACC+AGG + chr8_4:27581327-27581346 Msa1321550:CDS 40.0%
!! ACCAGGTCTTTTGTTTCCAA+AGG + chr8_4:27581344-27581363 Msa1321550:CDS 40.0%
! ACCTTTGGAAACAAAAGACC+TGG - chr8_4:27581348-27581367 None:intergenic 40.0%
CTGAAAAGAACAGACTGTGT+TGG - chr8_4:27581474-27581493 None:intergenic 40.0%
CCATAGGAAATGAGTCCATA+GGG - chr8_4:27581634-27581653 None:intergenic 40.0%
CCTATGGACTCATTTCCTAT+GGG + chr8_4:27581632-27581651 Msa1321550:intron 40.0%
! TTTGGAGCAATTCATCCCAT+AGG - chr8_4:27581650-27581669 None:intergenic 40.0%
TTGACTGATGTGGACGATAA+AGG + chr8_4:27581769-27581788 Msa1321550:intron 40.0%
CTATCAACCAACTAGCATGA+AGG - chr8_4:27581949-27581968 None:intergenic 40.0%
! TATCAAGGCTCAAGCTTTAG+AGG - chr8_4:27582184-27582203 None:intergenic 40.0%
TATACCCAGTGTATAAAGCG+TGG + chr8_4:27582309-27582328 Msa1321550:intron 40.0%
ATACCCAGTGTATAAAGCGT+GGG + chr8_4:27582310-27582329 Msa1321550:intron 40.0%
GAACATGACAACTCCACTTA+GGG - chr8_4:27582436-27582455 None:intergenic 40.0%
AGAACATGACAACTCCACTT+AGG - chr8_4:27582437-27582456 None:intergenic 40.0%
GGAGAATGATGAAACTGTTG+AGG + chr8_4:27582492-27582511 Msa1321550:CDS 40.0%
GAATGATGAAACTGTTGAGG+AGG + chr8_4:27582495-27582514 Msa1321550:CDS 40.0%
AGAGAAGCTATAGAAGAAGC+GGG + chr8_4:27582526-27582545 Msa1321550:CDS 40.0%
AATTGGACCCTATAATAGGG+TGG + chr8_4:27582827-27582846 Msa1321550:intron 40.0%
!! GCTGAAACCTTTTTGTTGTC+AGG + chr8_4:27583115-27583134 Msa1321550:intron 40.0%
TGGACTGAACTCATCTTGAT+GGG - chr8_4:27583174-27583193 None:intergenic 40.0%
AAGATGAGTTCAGTCCAGAA+GGG + chr8_4:27583177-27583196 Msa1321550:CDS 40.0%
!! GTTTGCTTTGTTTGTCAAGG+AGG + chr8_4:27583217-27583236 Msa1321550:CDS 40.0%
TCAAGGAGGAACTTGAACTA+TGG + chr8_4:27583231-27583250 Msa1321550:CDS 40.0%
ATGGCATGAGGAAAAGTTTG+TGG + chr8_4:27583355-27583374 Msa1321550:CDS 40.0%
CATGAGGAAAAGTTTGTGGA+TGG + chr8_4:27583359-27583378 Msa1321550:CDS 40.0%
CAGCAAATCAGTCTAACATC+AGG - chr8_4:27583399-27583418 None:intergenic 40.0%
ATTCTTGGGGTAGTTACTCT+AGG + chr8_4:27583487-27583506 Msa1321550:three_prime_UTR 40.0%
AGCGGTTAGAACGGATCAAT+GGG - chr8_4:27579256-27579275 None:intergenic 45.0%
AAGCGGTTAGAACGGATCAA+TGG - chr8_4:27579257-27579276 None:intergenic 45.0%
GAGAGAACGAAAAGGAGAAG+CGG - chr8_4:27579274-27579293 None:intergenic 45.0%
GGGAAAAGGAGAGAACGAAA+AGG - chr8_4:27579282-27579301 None:intergenic 45.0%
!! GTCTGATTTGGTTGCTCGTA+CGG + chr8_4:27579554-27579573 Msa1321550:CDS 45.0%
!! TCTGATTTGGTTGCTCGTAC+GGG + chr8_4:27579555-27579574 Msa1321550:CDS 45.0%
CATCAGCAACGCTATGAAGA+TGG + chr8_4:27579582-27579601 Msa1321550:CDS 45.0%
! GATGGTTATCGTCTTGTTGC+TGG + chr8_4:27579600-27579619 Msa1321550:CDS 45.0%
ATGAAGCACGGACACATACA+CGG + chr8_4:27579985-27580004 Msa1321550:intron 45.0%
! TTTCTAAGAACGCGCTACTC+TGG + chr8_4:27580634-27580653 Msa1321550:intron 45.0%
! GAAGATGTCAATTGGGTTGC+AGG + chr8_4:27581235-27581254 Msa1321550:intron 45.0%
AACAAAAGACCTGGTCCACT+AGG - chr8_4:27581339-27581358 None:intergenic 45.0%
CCCTATGGACTCATTTCCTA+TGG + chr8_4:27581631-27581650 Msa1321550:intron 45.0%
CCCATAGGAAATGAGTCCAT+AGG - chr8_4:27581635-27581654 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG + chr8_4:27582053-27582072 Msa1321550:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG + chr8_4:27582054-27582073 Msa1321550:intron 45.0%
TACCCAGTGTATAAAGCGTG+GGG + chr8_4:27582311-27582330 Msa1321550:intron 45.0%
TTCTCCCAACCTCCCTTTAA+TGG - chr8_4:27582478-27582497 None:intergenic 45.0%
TGATGAAACTGTTGAGGAGG+CGG + chr8_4:27582498-27582517 Msa1321550:CDS 45.0%
TAATGGGAACCTACGTTCTG+TGG + chr8_4:27582672-27582691 Msa1321550:intron 45.0%
TCAAATGCACCACAGAACGT+AGG - chr8_4:27582684-27582703 None:intergenic 45.0%
! ATGGGTGTGAAAGCACAAGA+AGG + chr8_4:27582780-27582799 Msa1321550:intron 45.0%
GATGGATCCTTTCTAACCGA+TGG + chr8_4:27583093-27583112 Msa1321550:intron 45.0%
! GTTTCAGCCATCGGTTAGAA+AGG - chr8_4:27583103-27583122 None:intergenic 45.0%
CTGGACTGAACTCATCTTGA+TGG - chr8_4:27583175-27583194 None:intergenic 45.0%
CAAGATGAGTTCAGTCCAGA+AGG + chr8_4:27583176-27583195 Msa1321550:CDS 45.0%
TACCAGAAATAGGAGTTGGC+TGG + chr8_4:27583265-27583284 Msa1321550:CDS 45.0%
ATCCAGCCAACTCCTATTTC+TGG - chr8_4:27583270-27583289 None:intergenic 45.0%
TAGGAGTTGGCTGGATGTAT+CGG + chr8_4:27583274-27583293 Msa1321550:CDS 45.0%
!! ATTAAATAAAAATAAAAAAG+GGG - chr8_4:27579639-27579658 None:intergenic 5.0%
!!! TAATTAATAATTATTGTTTT+AGG + chr8_4:27579672-27579691 Msa1321550:intron 5.0%
!!! AATTAATAATTATTGTTTTA+GGG + chr8_4:27579673-27579692 Msa1321550:intron 5.0%
ATTTGGTTGCTCGTACGGGT+CGG + chr8_4:27579559-27579578 Msa1321550:CDS 50.0%
GTTGCAGGTGTGTTCCCTTT+AGG + chr8_4:27581250-27581269 Msa1321550:intron 50.0%
CTTAGTGGGATAAGGCTTGG+TGG - chr8_4:27582052-27582071 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG - chr8_4:27582055-27582074 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG + chr8_4:27582052-27582071 Msa1321550:intron 50.0%
CAACCCCACTTAGTGGGATA+AGG - chr8_4:27582060-27582079 None:intergenic 50.0%
CTTATCCCACTAAGTGGGGT+TGG + chr8_4:27582058-27582077 Msa1321550:intron 50.0%
ACCCAGTGTATAAAGCGTGG+GGG + chr8_4:27582312-27582331 Msa1321550:intron 50.0%
ATAGAAGAAGCGGGAGTTCG+AGG + chr8_4:27582535-27582554 Msa1321550:CDS 50.0%
GCAGCTTTACACAACCCTTC+TGG - chr8_4:27583194-27583213 None:intergenic 50.0%
! TTGATAGCCTCCGACATGAG+TGG + chr8_4:27583303-27583322 Msa1321550:CDS 50.0%
!! CTCTGGAACGCTTCTGCAAA+TGG + chr8_4:27583336-27583355 Msa1321550:CDS 50.0%
ACGCTTCTGCAAATGGCATG+AGG + chr8_4:27583343-27583362 Msa1321550:CDS 50.0%
TCTGCGGAATGATGTCTGTC+CGG + chr8_4:27583549-27583568 Msa1321550:three_prime_UTR 50.0%
TGCAGCCAACCCCACTTAGT+GGG - chr8_4:27582066-27582085 None:intergenic 55.0%
ATGCAGCCAACCCCACTTAG+TGG - chr8_4:27582067-27582086 None:intergenic 55.0%
!! ACTAAGTGGGGTTGGCTGCA+TGG + chr8_4:27582066-27582085 Msa1321550:intron 55.0%
GCCCCCACGCTTTATACACT+GGG - chr8_4:27582316-27582335 None:intergenic 55.0%
AGCCCCCACGCTTTATACAC+TGG - chr8_4:27582317-27582336 None:intergenic 55.0%
GGGAGTTCGAGGAGACCTAA+TGG + chr8_4:27582546-27582565 Msa1321550:CDS 55.0%
GAGTTGGCTGGATGTATCGG+AGG + chr8_4:27583277-27583296 Msa1321550:CDS 55.0%
CCTCCGACATGAGTGGATGA+GGG + chr8_4:27583310-27583329 Msa1321550:CDS 55.0%
CGTCCCTCATCCACTCATGT+CGG - chr8_4:27583316-27583335 None:intergenic 55.0%
GCCTCCGACATGAGTGGATG+AGG + chr8_4:27583309-27583328 Msa1321550:CDS 60.0%
CCCTCATCCACTCATGTCGG+AGG - chr8_4:27583313-27583332 None:intergenic 60.0%
TGAGTGGATGAGGGACGCTC+TGG + chr8_4:27583319-27583338 Msa1321550:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 27579253 27583708 27579253 ID=Msa1321550;Name=Msa1321550
chr8_4 mRNA 27579253 27583708 27579253 ID=Msa1321550-mRNA-1;Parent=Msa1321550;Name=Msa1321550-mRNA-1;_AED=0.23;_eAED=0.23;_QI=270|1|1|1|0.75|0.6|5|317|172
chr8_4 exon 27579253 27579293 27579253 ID=Msa1321550-mRNA-1:exon:9236;Parent=Msa1321550-mRNA-1
chr8_4 exon 27579323 27579622 27579323 ID=Msa1321550-mRNA-1:exon:9237;Parent=Msa1321550-mRNA-1
chr8_4 exon 27581257 27581365 27581257 ID=Msa1321550-mRNA-1:exon:9238;Parent=Msa1321550-mRNA-1
chr8_4 exon 27582484 27582567 27582484 ID=Msa1321550-mRNA-1:exon:9239;Parent=Msa1321550-mRNA-1
chr8_4 exon 27583137 27583708 27583137 ID=Msa1321550-mRNA-1:exon:9240;Parent=Msa1321550-mRNA-1
chr8_4 five_prime_UTR 27579253 27579293 27579253 ID=Msa1321550-mRNA-1:five_prime_utr;Parent=Msa1321550-mRNA-1
chr8_4 five_prime_UTR 27579323 27579551 27579323 ID=Msa1321550-mRNA-1:five_prime_utr;Parent=Msa1321550-mRNA-1
chr8_4 CDS 27579552 27579622 27579552 ID=Msa1321550-mRNA-1:cds;Parent=Msa1321550-mRNA-1
chr8_4 CDS 27581257 27581365 27581257 ID=Msa1321550-mRNA-1:cds;Parent=Msa1321550-mRNA-1
chr8_4 CDS 27582484 27582567 27582484 ID=Msa1321550-mRNA-1:cds;Parent=Msa1321550-mRNA-1
chr8_4 CDS 27583137 27583391 27583137 ID=Msa1321550-mRNA-1:cds;Parent=Msa1321550-mRNA-1
chr8_4 three_prime_UTR 27583392 27583708 27583392 ID=Msa1321550-mRNA-1:three_prime_utr;Parent=Msa1321550-mRNA-1
Gene Sequence

>Msa1321550

ATGTCTGATTTGGTTGCTCGTACGGGTCGGCATCAGCAACGCTATGAAGATGGTTATCGTCTTGTTGCTGGGTGTGTTCCCTTTAGGTATAAAAGCTGTGAAGACGACTCTAGTTCCGAGAAGATTGTTGAAGTGCTTATGATTAATTCACCTAGTGGACCAGGTCTTTTGTTTCCAAAGGGAGGTTGGGAGAATGATGAAACTGTTGAGGAGGCGGCTGTAAGAGAAGCTATAGAAGAAGCGGGAGTTCGAGGAGACCTAATGGAATGTCTTGGATACTATGAGTTTAGGAGTAAGACCCATCAAGATGAGTTCAGTCCAGAAGGGTTGTGTAAAGCTGCAATGTTTGCTTTGTTTGTCAAGGAGGAACTTGAACTATGGCCTGAGCAAAATACCAGAAATAGGAGTTGGCTGGATGTATCGGAGGCACTTGATAGCCTCCGACATGAGTGGATGAGGGACGCTCTGGAACGCTTCTGCAAATGGCATGAGGAAAAGTTTGTGGATGGAACAGAATGA
Protein sequence

>Msa1321550

MSDLVARTGRHQQRYEDGYRLVAGCVPFRYKSCEDDSSSEKIVEVLMINSPSGPGLLFPKGGWENDETVEEAAVREAIEEAGVRGDLMECLGYYEFRSKTHQDEFSPEGLCKAAMFALFVKEELELWPEQNTRNRSWLDVSEALDSLRHEWMRDALERFCKWHEEKFVDGTE*