AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1345670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1345670 MtrunA17_Chr8g0353331 97.674 172 4 0 1 172 1 172 6.05e-126 351
Msa1345670 MtrunA17_Chr7g0252391 55.952 168 71 2 1 165 1 168 1.59e-58 182
Msa1345670 MtrunA17_Chr3g0110281 52.500 160 65 3 4 160 35 186 9.06e-50 159
Msa1345670 MtrunA17_Chr5g0434521 47.134 157 74 2 5 160 14 162 4.06e-44 144
Msa1345670 MtrunA17_Chr6g0479111 47.134 157 74 2 1 157 73 220 1.20e-43 144
Msa1345670 MtrunA17_Chr1g0161131 47.468 158 75 3 1 157 1 151 2.90e-42 139
Msa1345670 MtrunA17_Chr8g0391621 48.428 159 72 5 1 157 1 151 7.25e-42 137
Msa1345670 MtrunA17_Chr1g0189471 87.879 66 8 0 1 66 1 66 2.30e-39 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1345670 AT3G12600.1 72.000 175 46 2 1 172 1 175 4.33e-81 238
Msa1345670 AT3G12600.2 70.732 164 45 2 12 172 3 166 3.62e-74 220
Msa1345670 AT3G26690.1 57.143 168 65 4 1 161 1 168 2.12e-54 171
Msa1345670 AT3G26690.2 57.143 168 65 4 1 161 1 168 2.12e-54 171
Msa1345670 AT1G12880.1 54.070 172 73 3 1 166 1 172 2.12e-52 166
Msa1345670 AT1G18300.1 48.428 159 75 2 3 160 42 194 8.05e-48 154
Msa1345670 AT1G14860.1 50.311 161 73 3 1 161 1 154 3.81e-46 149
Msa1345670 AT2G01670.1 50.955 157 70 3 1 157 6 155 9.97e-46 148
Msa1345670 AT1G73540.1 47.500 160 71 4 3 160 43 191 1.16e-43 144

Find 69 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAACAACGAAGACATTT+TGG 0.236717 tig0007337:+19534 None:intergenic
GTTGCAGGTGTGTTCCCTTT+AGG 0.253270 tig0007337:-17633 Msa1345670:intron
TTTGATCAAAACACAATTCT+TGG 0.263035 tig0007337:-15413 Msa1345670:three_prime_UTR
ATTTCAATGGCGAAGGAAAT+TGG 0.279899 tig0007337:-19453 Msa1345670:five_prime_UTR
GCAGCTTTACACAACCCTTC+TGG 0.339256 tig0007337:+15694 None:intergenic
GATGGTTATCGTCTTGTTGC+TGG 0.345818 tig0007337:-19302 Msa1345670:CDS
GTCTGATTTGGTTGCTCGTA+CGG 0.347628 tig0007337:-19348 Msa1345670:CDS
ATCCAGCCAACTCCTATTTC+TGG 0.347905 tig0007337:+15618 None:intergenic
TTGATCAAAACACAATTCTT+GGG 0.351474 tig0007337:-15412 Msa1345670:three_prime_UTR
TGTTTCTATTATGTCTGATT+TGG 0.353265 tig0007337:-19360 Msa1345670:exon
TCTATCTATAATTGATATAC+TGG 0.356719 tig0007337:-15220 Msa1345670:three_prime_UTR
CTGAGCAAAATACCAGAAAT+AGG 0.370277 tig0007337:-15630 Msa1345670:CDS
CTGGACTGAACTCATCTTGA+TGG 0.375486 tig0007337:+15713 None:intergenic
TCAAGGAGGAACTTGAACTA+TGG 0.378915 tig0007337:-15654 Msa1345670:CDS
TTTGCTGACAATTTATTTGT+AGG 0.379791 tig0007337:-15446 Msa1345670:three_prime_UTR
TTGAAATCGAAATTACAATA+AGG 0.381048 tig0007337:+19469 None:intergenic
CTCTGGAACGCTTCTGCAAA+TGG 0.393459 tig0007337:-15549 Msa1345670:CDS
TATTGTAATTTCGATTTCAA+TGG 0.396745 tig0007337:-19466 Msa1345670:five_prime_UTR
TATATCAATTATAGATAGAT+AGG 0.397854 tig0007337:+15224 None:intergenic
GTCTTGGATACTATGAGTTT+AGG 0.399550 tig0007337:-15744 Msa1345670:CDS
TTGCTGACAATTTATTTGTA+GGG 0.401875 tig0007337:-15445 Msa1345670:three_prime_UTR
TCTGATTTGGTTGCTCGTAC+GGG 0.430251 tig0007337:-19347 Msa1345670:CDS
AAGCACTTCAACAATCTTCT+CGG 0.437562 tig0007337:+17582 None:intergenic
ATAGAAGAAGCGGGAGTTCG+AGG 0.477400 tig0007337:-16350 Msa1345670:CDS
AAGCGGTTAGAACGGATCAA+TGG 0.477919 tig0007337:+19648 None:intergenic
TGAGTGGATGAGGGACGCTC+TGG 0.483748 tig0007337:-15566 Msa1345670:CDS
ATTCTTGGGGTAGTTACTCT+AGG 0.495566 tig0007337:-15398 Msa1345670:three_prime_UTR
GCCTCCGACATGAGTGGATG+AGG 0.497019 tig0007337:-15576 Msa1345670:CDS
GGGAGTTCGAGGAGACCTAA+TGG 0.502057 tig0007337:-16339 Msa1345670:intron
TACCAGAAATAGGAGTTGGC+TGG 0.502905 tig0007337:-15620 Msa1345670:CDS
AAAATACCAGAAATAGGAGT+TGG 0.506282 tig0007337:-15624 Msa1345670:CDS
TAGGAGTTGGCTGGATGTAT+CGG 0.513352 tig0007337:-15611 Msa1345670:CDS
ATGGTTATCGTCTTGTTGCT+GGG 0.519282 tig0007337:-19301 Msa1345670:intron
AGCGGTTAGAACGGATCAAT+GGG 0.520314 tig0007337:+19649 None:intergenic
TGGACTGAACTCATCTTGAT+GGG 0.521294 tig0007337:+15714 None:intergenic
CAGCAAATCAGTCTAACATC+AGG 0.526302 tig0007337:+15489 None:intergenic
TCTGCGGAATGATGTCTGTC+CGG 0.527991 tig0007337:-15336 Msa1345670:three_prime_UTR
AATGTTTGCTTTGTTTGTCA+AGG 0.532894 tig0007337:-15671 Msa1345670:CDS
CATCAGCAACGCTATGAAGA+TGG 0.535027 tig0007337:-19320 Msa1345670:CDS
TGCTGACAATTTATTTGTAG+GGG 0.539264 tig0007337:-15444 Msa1345670:three_prime_UTR
CAGTTATTGTTTCAAGTCTG+CGG 0.546544 tig0007337:-15352 Msa1345670:three_prime_UTR
AAAAGGAGAAGCGGTTAGAA+CGG 0.546783 tig0007337:+19640 None:intergenic
ATGGCATGAGGAAAAGTTTG+TGG 0.551089 tig0007337:-15530 Msa1345670:CDS
CAAGATGAGTTCAGTCCAGA+AGG 0.552506 tig0007337:-15709 Msa1345670:CDS
ATTTGGTTGCTCGTACGGGT+CGG 0.565256 tig0007337:-19343 Msa1345670:CDS
AGAGAAGCTATAGAAGAAGC+GGG 0.567209 tig0007337:-16359 Msa1345670:CDS
AAGAGAAGCTATAGAAGAAG+CGG 0.568408 tig0007337:-16360 Msa1345670:CDS
AACAAAAGACCTGGTCCACT+AGG 0.578546 tig0007337:+17547 None:intergenic
TGATGAAACTGTTGAGGAGG+CGG 0.578996 tig0007337:-16387 Msa1345670:CDS
ATTAATTCACCTAGTGGACC+AGG 0.583292 tig0007337:-17556 Msa1345670:CDS
GTTTGCTTTGTTTGTCAAGG+AGG 0.584229 tig0007337:-15668 Msa1345670:CDS
GCTGACAATTTATTTGTAGG+GGG 0.592512 tig0007337:-15443 Msa1345670:three_prime_UTR
TGATCAAAACACAATTCTTG+GGG 0.601836 tig0007337:-15411 Msa1345670:three_prime_UTR
CTTATGATTAATTCACCTAG+TGG 0.602210 tig0007337:-17562 Msa1345670:CDS
ACCTTTGGAAACAAAAGACC+TGG 0.602229 tig0007337:+17538 None:intergenic
CCCTCATCCACTCATGTCGG+AGG 0.604589 tig0007337:+15575 None:intergenic
GAATGATGAAACTGTTGAGG+AGG 0.612719 tig0007337:-16390 Msa1345670:CDS
AATTTCGATTTCAATGGCGA+AGG 0.612949 tig0007337:-19460 Msa1345670:five_prime_UTR
TTGATAGCCTCCGACATGAG+TGG 0.618790 tig0007337:-15582 Msa1345670:CDS
CATGAGGAAAAGTTTGTGGA+TGG 0.623665 tig0007337:-15526 Msa1345670:CDS
ACGCTTCTGCAAATGGCATG+AGG 0.629815 tig0007337:-15542 Msa1345670:CDS
CGTCCCTCATCCACTCATGT+CGG 0.636052 tig0007337:+15572 None:intergenic
GAGAGAACGAAAAGGAGAAG+CGG 0.638095 tig0007337:+19631 None:intergenic
CCTCCGACATGAGTGGATGA+GGG 0.641158 tig0007337:-15575 Msa1345670:CDS
GTTACTCTGAATCAAAAGAC+CGG 0.645377 tig0007337:+15317 None:intergenic
GAGTTGGCTGGATGTATCGG+AGG 0.659992 tig0007337:-15608 Msa1345670:CDS
AAGATGAGTTCAGTCCAGAA+GGG 0.667305 tig0007337:-15708 Msa1345670:CDS
CAATTATAGATAGATAGGTG+TGG 0.670020 tig0007337:+15229 None:intergenic
GGAGAATGATGAAACTGTTG+AGG 0.693121 tig0007337:-16393 Msa1345670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAATAAAAATAAAAAA+AGG + tig0007337:15587-15606 None:intergenic 0.0%
!! TAAATAAAAATAAAAAAAGG+AGG + tig0007337:15584-15603 None:intergenic 10.0%
!!! TAATTAATAATCATTGTTTT+AGG - tig0007337:15619-15638 Msa1345670:CDS 10.0%
!!! AATTAATAATCATTGTTTTA+GGG - tig0007337:15620-15639 Msa1345670:CDS 10.0%
!! ATATTTGAATGATTAATATT+TGG - tig0007337:16093-16112 Msa1345670:intron 10.0%
!!! TTTATAAAGAAGTATTATAA+AGG - tig0007337:16329-16348 Msa1345670:intron 10.0%
!!! TTGAGAATTTTTTTTGAATT+TGG - tig0007337:15432-15451 Msa1345670:three_prime_UTR 15.0%
!! TTGGATTTGATTAATAAAAA+AGG - tig0007337:15451-15470 Msa1345670:three_prime_UTR 15.0%
!! TGGATTTGATTAATAAAAAA+GGG - tig0007337:15452-15471 Msa1345670:three_prime_UTR 15.0%
!!! ATTAATAATCATTGTTTTAG+GGG - tig0007337:15621-15640 Msa1345670:CDS 15.0%
!!! TTAATTTGATGATATATGAA+TGG - tig0007337:15861-15880 Msa1345670:intron 15.0%
!! AATTATGAAATGAATTTGAT+TGG - tig0007337:17022-17041 Msa1345670:intron 15.0%
!!! TAAAAGTTGATTAATGAAAA+AGG + tig0007337:17087-17106 None:intergenic 15.0%
!!! TGATAAAAATATTTTCAGAT+AGG - tig0007337:17110-17129 Msa1345670:intron 15.0%
!!! TGATATTTTAAAATTTGCAA+AGG - tig0007337:17414-17433 Msa1345670:intron 15.0%
!!! AAAAATTTCACTTATTTCTT+TGG + tig0007337:17633-17652 None:intergenic 15.0%
!!! AATAAGTGAAATTTTTCTTA+TGG - tig0007337:17637-17656 Msa1345670:intron 15.0%
!!! ATACATATATATTTTGAAGA+AGG - tig0007337:17857-17876 Msa1345670:intron 15.0%
!!! TACATATATATTTTGAAGAA+GGG - tig0007337:17858-17877 Msa1345670:intron 15.0%
!!! ACATTTAAAATATGATGAAT+TGG - tig0007337:18773-18792 Msa1345670:intron 15.0%
!!! TGTTATTTTAATTGTAAACA+AGG - tig0007337:18991-19010 Msa1345670:intron 15.0%
!! TATATCAATTATAGATAGAT+AGG + tig0007337:19627-19646 None:intergenic 15.0%
!! TTGAAATCGAAATTACAATA+AGG + tig0007337:15382-15401 None:intergenic 20.0%
!! TATTGTAATTTCGATTTCAA+TGG - tig0007337:15382-15401 Msa1345670:three_prime_UTR 20.0%
!!! GAAAAGAATGTTTTTTGTTT+TGG - tig0007337:15664-15683 Msa1345670:CDS 20.0%
!!! TTTATTTGTGTGTTTGTTTT+TGG - tig0007337:15734-15753 Msa1345670:CDS 20.0%
!!! TCTCTATTATGGTTTTTTAA+GGG - tig0007337:15791-15810 Msa1345670:intron 20.0%
!! AATGGCTGAAATTAAATTTA+AGG - tig0007337:15879-15898 Msa1345670:intron 20.0%
!! AAAGAAGAAGAAAAAAAACA+TGG + tig0007337:16403-16422 None:intergenic 20.0%
!! TGCTAAATTGATTAAAACAA+TGG - tig0007337:16471-16490 Msa1345670:intron 20.0%
!! GTTCTTAGAAAACTAAAAAT+AGG + tig0007337:16587-16606 None:intergenic 20.0%
!! GGAAAATAATAAACAAAGAT+AGG + tig0007337:16772-16791 None:intergenic 20.0%
!!! TAAATGTAATTTTAATCCTC+TGG - tig0007337:16848-16867 Msa1345670:intron 20.0%
!!! TTTTTCTTATATACCTGTTT+TGG - tig0007337:17151-17170 Msa1345670:intron 20.0%
!! TATTTCAAACATTATACCTT+TGG + tig0007337:17328-17347 None:intergenic 20.0%
!!! TTCTTTTCAGTCATTATATT+GGG - tig0007337:17449-17468 Msa1345670:intron 20.0%
!! ACCTCTTATACTAATTATAA+TGG - tig0007337:17503-17522 Msa1345670:intron 20.0%
!! ACCATTATAATTAGTATAAG+AGG + tig0007337:17507-17526 None:intergenic 20.0%
!!! TCTTTCTAATTAAAAGTATC+AGG + tig0007337:17664-17683 None:intergenic 20.0%
!!! TTGTACTAAAATTCTTTTCT+TGG - tig0007337:17709-17728 Msa1345670:intron 20.0%
!! TTCTCTAATATCTACTATTA+TGG + tig0007337:18069-18088 None:intergenic 20.0%
!!! TTTTAAACAGTAACTTAATC+TGG - tig0007337:18340-18359 Msa1345670:intron 20.0%
!!! ATATTTTGATGATCTCATAA+TGG - tig0007337:18618-18637 Msa1345670:intron 20.0%
!!! TATTTTGATGATCTCATAAT+GGG - tig0007337:18619-18638 Msa1345670:intron 20.0%
!! TCTATCTATAATTGATATAC+TGG - tig0007337:19628-19647 Msa1345670:intron 20.0%
!! TGTTTCTATTATGTCTGATT+TGG - tig0007337:15488-15507 Msa1345670:three_prime_UTR 25.0%
!!! GTCTCTATTATGGTTTTTTA+AGG - tig0007337:15790-15809 Msa1345670:intron 25.0%
!!! CTCTATTATGGTTTTTTAAG+GGG - tig0007337:15792-15811 Msa1345670:intron 25.0%
! CATATGAAAGTCAATGTATA+AGG - tig0007337:15817-15836 Msa1345670:intron 25.0%
!!! ATTTTTATGGTCAACTTGTT+AGG - tig0007337:16216-16235 Msa1345670:intron 25.0%
!!! TTTTTATGGTCAACTTGTTA+GGG - tig0007337:16217-16236 Msa1345670:intron 25.0%
!! CATTTTCACAAAATAGGATT+AGG - tig0007337:16241-16260 Msa1345670:intron 25.0%
! AAAATAGGATTAGGATTACT+TGG - tig0007337:16250-16269 Msa1345670:intron 25.0%
!! TAATTAGTCTATTTAGCTAC+AGG + tig0007337:16293-16312 None:intergenic 25.0%
! AGCTATAAAATACAACTCTA+AGG + tig0007337:16624-16643 None:intergenic 25.0%
! TAAGACAGATACTAAACAAT+TGG + tig0007337:16945-16964 None:intergenic 25.0%
! AATACTAAAAAGTAAACGAC+TGG - tig0007337:16980-16999 Msa1345670:intron 25.0%
! AAATCACTCTAACTACAAAT+GGG - tig0007337:17051-17070 Msa1345670:intron 25.0%
!! GTTCTTTTCAGTCATTATAT+TGG - tig0007337:17448-17467 Msa1345670:intron 25.0%
! AGTCATTATATTGGGAAATT+TGG - tig0007337:17457-17476 Msa1345670:intron 25.0%
!!! ACAGAACTGATTTGTAAATT+TGG - tig0007337:17553-17572 Msa1345670:CDS 25.0%
! TTGAAAACTAGTTAGGTAAA+TGG - tig0007337:17960-17979 Msa1345670:intron 25.0%
! GTTTAGGTAATAAAATAGCT+TGG - tig0007337:18201-18220 Msa1345670:intron 25.0%
! ATGAGCATTTCCTAAATTAT+TGG + tig0007337:18250-18269 None:intergenic 25.0%
! AATTCATATTTCTCACCATT+AGG + tig0007337:18527-18546 None:intergenic 25.0%
! TAGCTTCATTAATATCATGA+TGG - tig0007337:18581-18600 Msa1345670:intron 25.0%
! AGCTTCATTAATATCATGAT+GGG - tig0007337:18582-18601 Msa1345670:intron 25.0%
!! AAGTTTTCTTAACTTTGCAT+GGG - tig0007337:18725-18744 Msa1345670:intron 25.0%
! TAATATTCCACCCTATTATA+GGG + tig0007337:18800-18819 None:intergenic 25.0%
! ATAATATTCCACCCTATTAT+AGG + tig0007337:18801-18820 None:intergenic 25.0%
!! TTCTACTTTCTACATGATTT+TGG - tig0007337:18878-18897 Msa1345670:intron 25.0%
! TTTGCTGACAATTTATTTGT+AGG - tig0007337:19402-19421 Msa1345670:five_prime_UTR 25.0%
! TTGCTGACAATTTATTTGTA+GGG - tig0007337:19403-19422 Msa1345670:five_prime_UTR 25.0%
! TTTGATCAAAACACAATTCT+TGG - tig0007337:19435-19454 Msa1345670:five_prime_UTR 25.0%
! TTGATCAAAACACAATTCTT+GGG - tig0007337:19436-19455 Msa1345670:five_prime_UTR 25.0%
AAGATTGAAGAAATGGGAAA+AGG + tig0007337:15242-15261 None:intergenic 30.0%
AGAGAGAAGATTGAAGAAAT+GGG + tig0007337:15248-15267 None:intergenic 30.0%
!!! CGAAGACATTTTGGTTTTTA+GGG + tig0007337:15308-15327 None:intergenic 30.0%
!!! ACGAAGACATTTTGGTTTTT+AGG + tig0007337:15309-15328 None:intergenic 30.0%
!!! GGAAACATGTCATTTTCTTA+CGG - tig0007337:15642-15661 Msa1345670:CDS 30.0%
GTGTTTGAGAAATTACGATT+TGG - tig0007337:15686-15705 Msa1345670:CDS 30.0%
!!! TTACGATTTGGTTTTGTTGA+TGG - tig0007337:15698-15717 Msa1345670:CDS 30.0%
!!! TTTTGTTGATGGTGATGTTT+TGG - tig0007337:15709-15728 Msa1345670:CDS 30.0%
GTTGATGTATGTCTCTATTA+TGG - tig0007337:15780-15799 Msa1345670:intron 30.0%
AAAGTCAATGTATAAGGTGA+AGG - tig0007337:15823-15842 Msa1345670:intron 30.0%
TGATTATATGCTATGAAGCA+CGG - tig0007337:15921-15940 Msa1345670:intron 30.0%
AATGATGTCAGAAAATGACA+TGG - tig0007337:15978-15997 Msa1345670:intron 30.0%
! ATGTAATGACTTGTGATGTT+AGG - tig0007337:16005-16024 Msa1345670:intron 30.0%
!!! GGTCTTGCTTTTTATGTTTT+AGG - tig0007337:16114-16133 Msa1345670:intron 30.0%
!!! TTTTATGGTCAACTTGTTAG+GGG - tig0007337:16218-16237 Msa1345670:intron 30.0%
! CATGCATATTTGATGATTGA+TGG - tig0007337:16374-16393 Msa1345670:CDS 30.0%
TAGATTGTATCTGTTGTGAT+AGG - tig0007337:16445-16464 Msa1345670:intron 30.0%
ATTAAAACAATGGATGAGTC+TGG - tig0007337:16481-16500 Msa1345670:intron 30.0%
TTATGCATTATGACAACGTT+TGG - tig0007337:16643-16662 Msa1345670:intron 30.0%
TACATGTATCAGTAACATGT+AGG + tig0007337:16721-16740 None:intergenic 30.0%
GAATGAAACTATTACATGCT+GGG + tig0007337:16793-16812 None:intergenic 30.0%
AGAATGAAACTATTACATGC+TGG + tig0007337:16794-16813 None:intergenic 30.0%
GAAATCACTCTAACTACAAA+TGG - tig0007337:17050-17069 Msa1345670:intron 30.0%
TCTTCAAAACAGAACTTGAA+GGG + tig0007337:17185-17204 None:intergenic 30.0%
ATCTTCAAAACAGAACTTGA+AGG + tig0007337:17186-17205 None:intergenic 30.0%
!!! TCTGTTTTGAAGATGTCAAT+TGG - tig0007337:17192-17211 Msa1345670:intron 30.0%
!!! CTGTTTTGAAGATGTCAATT+GGG - tig0007337:17193-17212 Msa1345670:intron 30.0%
! TCACAGCTTTTATACCTAAA+GGG + tig0007337:17232-17251 None:intergenic 30.0%
! TTCACAGCTTTTATACCTAA+AGG + tig0007337:17233-17252 None:intergenic 30.0%
CTTATGATTAATTCACCTAG+TGG - tig0007337:17286-17305 Msa1345670:intron 30.0%
AATCAGTTCTGTTGTAATGA+TGG + tig0007337:17545-17564 None:intergenic 30.0%
!!! AATTGGTTTTACTATCCCTA+TGG - tig0007337:17581-17600 Msa1345670:CDS 30.0%
AAGAGAATATCATGTGCATT+AGG - tig0007337:17680-17699 Msa1345670:intron 30.0%
TTTGTTTCCAAAGATGACTT+AGG - tig0007337:17802-17821 Msa1345670:intron 30.0%
TAAAATACCTTCATGCTAGT+TGG - tig0007337:17904-17923 Msa1345670:intron 30.0%
AATTAGGTTCGACTATATCA+AGG + tig0007337:18164-18183 None:intergenic 30.0%
!! GTTTTGAGCTCCAATAATTT+AGG - tig0007337:18237-18256 Msa1345670:intron 30.0%
!!! GGTTTGCGTTGTTTTTTTAA+TGG - tig0007337:18372-18391 Msa1345670:intron 30.0%
!!! TTTTTTTAATGGACCCTAAG+TGG - tig0007337:18383-18402 Msa1345670:intron 30.0%
!!! TTGCGTTGTTTTTCCATTAA+AGG - tig0007337:18425-18444 Msa1345670:intron 30.0%
!!! TGCGTTGTTTTTCCATTAAA+GGG - tig0007337:18426-18445 Msa1345670:intron 30.0%
TATTCTTTGTTGCTGTAAGT+TGG - tig0007337:18691-18710 Msa1345670:intron 30.0%
! GAAGTTTTCTTAACTTTGCA+TGG - tig0007337:18724-18743 Msa1345670:intron 30.0%
ATGAATTGGACCCTATAATA+GGG - tig0007337:18787-18806 Msa1345670:intron 30.0%
!! CTTTCAAGTTACAACAGTAA+AGG + tig0007337:18944-18963 None:intergenic 30.0%
!!! TATCGATCTTGTCATTTTGA+TGG - tig0007337:19038-19057 Msa1345670:intron 30.0%
!! AATGTTTGCTTTGTTTGTCA+AGG - tig0007337:19177-19196 Msa1345670:intron 30.0%
AAAATACCAGAAATAGGAGT+TGG - tig0007337:19224-19243 Msa1345670:intron 30.0%
TGCTGACAATTTATTTGTAG+GGG - tig0007337:19404-19423 Msa1345670:five_prime_UTR 30.0%
TGATCAAAACACAATTCTTG+GGG - tig0007337:19437-19456 Msa1345670:five_prime_UTR 30.0%
CAATTATAGATAGATAGGTG+TGG + tig0007337:19622-19641 None:intergenic 30.0%
GAGAGAGAAGATTGAAGAAA+TGG + tig0007337:15249-15268 None:intergenic 35.0%
!!! GTTTTTAGGGTTTGCATAAG+AGG + tig0007337:15295-15314 None:intergenic 35.0%
! TCTCAACAACGAAGACATTT+TGG + tig0007337:15317-15336 None:intergenic 35.0%
AATTTCGATTTCAATGGCGA+AGG - tig0007337:15388-15407 Msa1345670:three_prime_UTR 35.0%
ATTTCAATGGCGAAGGAAAT+TGG - tig0007337:15395-15414 Msa1345670:three_prime_UTR 35.0%
!!! TGTGTGTTTGTTTTTGGTTG+TGG - tig0007337:15740-15759 Msa1345670:CDS 35.0%
!!! TTGTTTTTGGTTGTGGAAGA+TGG - tig0007337:15747-15766 Msa1345670:CDS 35.0%
! ACACCTTTGATTAGAAGTGT+CGG - tig0007337:16043-16062 Msa1345670:intron 35.0%
GATGAAACTTGTGTTGCATA+TGG - tig0007337:16178-16197 Msa1345670:intron 35.0%
!!! TAGGGGCATTTTCACAAAAT+AGG - tig0007337:16235-16254 Msa1345670:intron 35.0%
!! GACTAATTAATGAAGCACTG+AGG - tig0007337:16304-16323 Msa1345670:intron 35.0%
AAGTTCAGTTGAATCGAATG+TGG - tig0007337:16667-16686 Msa1345670:intron 35.0%
TCAGTAACATGTAGGTACAA+AGG + tig0007337:16713-16732 None:intergenic 35.0%
TGTAGTCCTGGAAATATCAA+TGG - tig0007337:16737-16756 Msa1345670:intron 35.0%
CTTATGCCATTGATATTTCC+AGG + tig0007337:16746-16765 None:intergenic 35.0%
CAAGATAAGTCAAAAACCAG+AGG + tig0007337:16867-16886 None:intergenic 35.0%
!!! TGGTTTTTGACTTATCTTGC+TGG - tig0007337:16868-16887 Msa1345670:intron 35.0%
!! AAGGGTATCATATCCAAAAC+AGG + tig0007337:17167-17186 None:intergenic 35.0%
AAGCACTTCAACAATCTTCT+CGG + tig0007337:17269-17288 None:intergenic 35.0%
! AACCTGTTTGAGTATTGTTG+TGG - tig0007337:17358-17377 Msa1345670:intron 35.0%
AACCACAACAATACTCAAAC+AGG + tig0007337:17363-17382 None:intergenic 35.0%
! GTTGTGGTTGATACTAGTTT+AGG - tig0007337:17374-17393 Msa1345670:intron 35.0%
TTCTGTTGTAATGATGGCTA+CGG + tig0007337:17539-17558 None:intergenic 35.0%
TTGTAAATTTGGACGCGAAT+TGG - tig0007337:17564-17583 Msa1345670:CDS 35.0%
TTTCTTGGAATTGACTGATG+TGG - tig0007337:17724-17743 Msa1345670:intron 35.0%
ATGTGGACGATAAAGGATTA+TGG - tig0007337:17741-17760 Msa1345670:intron 35.0%
CTCTAATCCTAAGTCATCTT+TGG + tig0007337:17812-17831 None:intergenic 35.0%
GATAGTCACAAACTGAACAT+AGG - tig0007337:17933-17952 Msa1345670:intron 35.0%
AGGACTCTTGAAAACTAGTT+AGG - tig0007337:17953-17972 Msa1345670:intron 35.0%
AACAAGCTCAAGCTTGAATT+AGG + tig0007337:18180-18199 None:intergenic 35.0%
! AAGCTTGAGCTTGTTTGTTT+AGG - tig0007337:18185-18204 Msa1345670:intron 35.0%
AACTTAATCTGGTTGAGATC+AGG - tig0007337:18351-18370 Msa1345670:intron 35.0%
!!! GTTGTTTTTCCATTAAAGGG+AGG - tig0007337:18429-18448 Msa1345670:intron 35.0%
! TTTTTCCATTAAAGGGAGGT+TGG - tig0007337:18433-18452 Msa1345670:intron 35.0%
! TTTTCCATTAAAGGGAGGTT+GGG - tig0007337:18434-18453 Msa1345670:intron 35.0%
AAGAGAAGCTATAGAAGAAG+CGG - tig0007337:18488-18507 Msa1345670:intron 35.0%
!! ACTTTAGAACTTCTGCAATG+TGG - tig0007337:18552-18571 Msa1345670:intron 35.0%
GATGAATTGGACCCTATAAT+AGG - tig0007337:18786-18805 Msa1345670:intron 35.0%
! CTCACATCTGAAATAAATGC+TGG + tig0007337:18845-18864 None:intergenic 35.0%
AACAAAAAGGTTTCAGCCAT+CGG + tig0007337:19075-19094 None:intergenic 35.0%
AGACATTCCTGACAACAAAA+AGG + tig0007337:19088-19107 None:intergenic 35.0%
!! TTTTGTTGTCAGGAATGTCT+TGG - tig0007337:19088-19107 Msa1345670:intron 35.0%
GTCTTGGATACTATGAGTTT+AGG - tig0007337:19104-19123 Msa1345670:intron 35.0%
!!! CTATTTCTGGTATTTTGCTC+AGG + tig0007337:19220-19239 None:intergenic 35.0%
CTGAGCAAAATACCAGAAAT+AGG - tig0007337:19218-19237 Msa1345670:intron 35.0%
GCTGACAATTTATTTGTAGG+GGG - tig0007337:19405-19424 Msa1345670:five_prime_UTR 35.0%
! CAGTTATTGTTTCAAGTCTG+CGG - tig0007337:19496-19515 Msa1345670:five_prime_UTR 35.0%
GTTACTCTGAATCAAAAGAC+CGG + tig0007337:19534-19553 None:intergenic 35.0%
AAAAGGAGAAGCGGTTAGAA+CGG + tig0007337:15211-15230 None:intergenic 40.0%
! ATGGTTATCGTCTTGTTGCT+GGG - tig0007337:15547-15566 Msa1345670:CDS 40.0%
GTCAATGTATAAGGTGAAGG+TGG - tig0007337:15826-15845 Msa1345670:intron 40.0%
TGATGTTAGGAGTCAGATAC+TGG - tig0007337:16018-16037 Msa1345670:intron 40.0%
AACAATGGATGAGTCTGGAT+TGG - tig0007337:16486-16505 Msa1345670:intron 40.0%
GTTACTGATACATGTAGTCC+TGG - tig0007337:16725-16744 Msa1345670:intron 40.0%
! ATTAATTCACCTAGTGGACC+AGG - tig0007337:17292-17311 Msa1345670:intron 40.0%
!! ACCAGGTCTTTTGTTTCCAA+AGG - tig0007337:17309-17328 Msa1345670:intron 40.0%
! ACCTTTGGAAACAAAAGACC+TGG + tig0007337:17313-17332 None:intergenic 40.0%
CCATAGGAAATGAGTCCATA+GGG + tig0007337:17599-17618 None:intergenic 40.0%
CCTATGGACTCATTTCCTAT+GGG - tig0007337:17597-17616 Msa1345670:CDS 40.0%
! TTTGGAGCAATTCATCCCAT+AGG + tig0007337:17615-17634 None:intergenic 40.0%
TTGACTGATGTGGACGATAA+AGG - tig0007337:17734-17753 Msa1345670:intron 40.0%
CTATCAACCAACTAGCATGA+AGG + tig0007337:17914-17933 None:intergenic 40.0%
! TATCAAGGCTCAAGCTTTAG+AGG + tig0007337:18149-18168 None:intergenic 40.0%
GAACATGACAACTCCACTTA+GGG + tig0007337:18399-18418 None:intergenic 40.0%
AGAACATGACAACTCCACTT+AGG + tig0007337:18400-18419 None:intergenic 40.0%
GGAGAATGATGAAACTGTTG+AGG - tig0007337:18455-18474 Msa1345670:intron 40.0%
GAATGATGAAACTGTTGAGG+AGG - tig0007337:18458-18477 Msa1345670:intron 40.0%
AGAGAAGCTATAGAAGAAGC+GGG - tig0007337:18489-18508 Msa1345670:intron 40.0%
AATTGGACCCTATAATAGGG+TGG - tig0007337:18790-18809 Msa1345670:intron 40.0%
!! GCTGAAACCTTTTTGTTGTC+AGG - tig0007337:19078-19097 Msa1345670:intron 40.0%
TGGACTGAACTCATCTTGAT+GGG + tig0007337:19137-19156 None:intergenic 40.0%
AAGATGAGTTCAGTCCAGAA+GGG - tig0007337:19140-19159 Msa1345670:intron 40.0%
!! GTTTGCTTTGTTTGTCAAGG+AGG - tig0007337:19180-19199 Msa1345670:intron 40.0%
TCAAGGAGGAACTTGAACTA+TGG - tig0007337:19194-19213 Msa1345670:intron 40.0%
ATGGCATGAGGAAAAGTTTG+TGG - tig0007337:19318-19337 Msa1345670:CDS 40.0%
CATGAGGAAAAGTTTGTGGA+TGG - tig0007337:19322-19341 Msa1345670:CDS 40.0%
CAGCAAATCAGTCTAACATC+AGG + tig0007337:19362-19381 None:intergenic 40.0%
ATTCTTGGGGTAGTTACTCT+AGG - tig0007337:19450-19469 Msa1345670:five_prime_UTR 40.0%
AGCGGTTAGAACGGATCAAT+GGG + tig0007337:15202-15221 None:intergenic 45.0%
AAGCGGTTAGAACGGATCAA+TGG + tig0007337:15203-15222 None:intergenic 45.0%
GAGAGAACGAAAAGGAGAAG+CGG + tig0007337:15220-15239 None:intergenic 45.0%
GGGAAAAGGAGAGAACGAAA+AGG + tig0007337:15228-15247 None:intergenic 45.0%
!! GTCTGATTTGGTTGCTCGTA+CGG - tig0007337:15500-15519 Msa1345670:exon 45.0%
!! TCTGATTTGGTTGCTCGTAC+GGG - tig0007337:15501-15520 Msa1345670:exon 45.0%
CATCAGCAACGCTATGAAGA+TGG - tig0007337:15528-15547 Msa1345670:CDS 45.0%
! GATGGTTATCGTCTTGTTGC+TGG - tig0007337:15546-15565 Msa1345670:CDS 45.0%
ATGAAGCACGGACACATACA+CGG - tig0007337:15933-15952 Msa1345670:intron 45.0%
! GCACCGACACTTCTAATCAA+AGG + tig0007337:16049-16068 None:intergenic 45.0%
! TTTCTAAGAACGCGCTACTC+TGG - tig0007337:16596-16615 Msa1345670:intron 45.0%
! GAAGATGTCAATTGGGTTGC+AGG - tig0007337:17200-17219 Msa1345670:intron 45.0%
AACAAAAGACCTGGTCCACT+AGG + tig0007337:17304-17323 None:intergenic 45.0%
CCCTATGGACTCATTTCCTA+TGG - tig0007337:17596-17615 Msa1345670:CDS 45.0%
CCCATAGGAAATGAGTCCAT+AGG + tig0007337:17600-17619 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG - tig0007337:18018-18037 Msa1345670:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - tig0007337:18019-18038 Msa1345670:intron 45.0%
TCTCATATACCCAGTGTACG+TGG - tig0007337:18269-18288 Msa1345670:intron 45.0%
CTCATATACCCAGTGTACGT+GGG - tig0007337:18270-18289 Msa1345670:intron 45.0%
TCATATACCCAGTGTACGTG+GGG - tig0007337:18271-18290 Msa1345670:intron 45.0%
TTCTCCCAACCTCCCTTTAA+TGG + tig0007337:18441-18460 None:intergenic 45.0%
TGATGAAACTGTTGAGGAGG+CGG - tig0007337:18461-18480 Msa1345670:intron 45.0%
TAATGGGAACCTACGTTCTG+TGG - tig0007337:18635-18654 Msa1345670:intron 45.0%
TCAAATGCACCACAGAACGT+AGG + tig0007337:18647-18666 None:intergenic 45.0%
! ATGGGTGTGAAAGCACAAGA+AGG - tig0007337:18743-18762 Msa1345670:intron 45.0%
GATGGATCCTTTCTAACCGA+TGG - tig0007337:19056-19075 Msa1345670:intron 45.0%
! GTTTCAGCCATCGGTTAGAA+AGG + tig0007337:19066-19085 None:intergenic 45.0%
CTGGACTGAACTCATCTTGA+TGG + tig0007337:19138-19157 None:intergenic 45.0%
CAAGATGAGTTCAGTCCAGA+AGG - tig0007337:19139-19158 Msa1345670:intron 45.0%
TACCAGAAATAGGAGTTGGC+TGG - tig0007337:19228-19247 Msa1345670:intron 45.0%
ATCCAGCCAACTCCTATTTC+TGG + tig0007337:19233-19252 None:intergenic 45.0%
TAGGAGTTGGCTGGATGTAT+CGG - tig0007337:19237-19256 Msa1345670:intron 45.0%
!!! AATATATATGTATATTTTTA+TGG - tig0007337:16203-16222 Msa1345670:intron 5.0%
ATTTGGTTGCTCGTACGGGT+CGG - tig0007337:15505-15524 Msa1345670:exon 50.0%
GTTGCAGGTGTGTTCCCTTT+AGG - tig0007337:17215-17234 Msa1345670:intron 50.0%
CTTAGTGGGATAAGGCTTGG+TGG + tig0007337:18017-18036 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG + tig0007337:18020-18039 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG - tig0007337:18017-18036 Msa1345670:intron 50.0%
CAACCCCACTTAGTGGGATA+AGG + tig0007337:18025-18044 None:intergenic 50.0%
CTTATCCCACTAAGTGGGGT+TGG - tig0007337:18023-18042 Msa1345670:intron 50.0%
CATATACCCAGTGTACGTGG+GGG - tig0007337:18272-18291 Msa1345670:intron 50.0%
ATAAAGCCCCCACGTACACT+GGG + tig0007337:18281-18300 None:intergenic 50.0%
AATAAAGCCCCCACGTACAC+TGG + tig0007337:18282-18301 None:intergenic 50.0%
ATAGAAGAAGCGGGAGTTCG+AGG - tig0007337:18498-18517 Msa1345670:intron 50.0%
GCAGCTTTACACAACCCTTC+TGG + tig0007337:19157-19176 None:intergenic 50.0%
! TTGATAGCCTCCGACATGAG+TGG - tig0007337:19266-19285 Msa1345670:intron 50.0%
!! CTCTGGAACGCTTCTGCAAA+TGG - tig0007337:19299-19318 Msa1345670:intron 50.0%
ACGCTTCTGCAAATGGCATG+AGG - tig0007337:19306-19325 Msa1345670:CDS 50.0%
TCTGCGGAATGATGTCTGTC+CGG - tig0007337:19512-19531 Msa1345670:five_prime_UTR 50.0%
TGCAGCCAACCCCACTTAGT+GGG + tig0007337:18031-18050 None:intergenic 55.0%
ATGCAGCCAACCCCACTTAG+TGG + tig0007337:18032-18051 None:intergenic 55.0%
!! ACTAAGTGGGGTTGGCTGCA+TGG - tig0007337:18031-18050 Msa1345670:intron 55.0%
GGGAGTTCGAGGAGACCTAA+TGG - tig0007337:18509-18528 Msa1345670:intron 55.0%
GAGTTGGCTGGATGTATCGG+AGG - tig0007337:19240-19259 Msa1345670:intron 55.0%
CCTCCGACATGAGTGGATGA+GGG - tig0007337:19273-19292 Msa1345670:intron 55.0%
CGTCCCTCATCCACTCATGT+CGG + tig0007337:19279-19298 None:intergenic 55.0%
GCCTCCGACATGAGTGGATG+AGG - tig0007337:19272-19291 Msa1345670:intron 60.0%
CCCTCATCCACTCATGTCGG+AGG + tig0007337:19276-19295 None:intergenic 60.0%
TGAGTGGATGAGGGACGCTC+TGG - tig0007337:19282-19301 Msa1345670:intron 60.0%
Chromosome Type Strat End Strand Name
tig0007337 gene 15199 19671 15199 ID=Msa1345670;Name=Msa1345670
tig0007337 mRNA 15199 19671 15199 ID=Msa1345670-mRNA-1;Parent=Msa1345670;Name=Msa1345670-mRNA-1;_AED=0.23;_eAED=0.23;_QI=270|1|1|1|0.75|0.6|5|317|172
tig0007337 exon 15199 15770 15199 ID=Msa1345670-mRNA-1:exon:5123;Parent=Msa1345670-mRNA-1
tig0007337 exon 16340 16423 16340 ID=Msa1345670-mRNA-1:exon:5122;Parent=Msa1345670-mRNA-1
tig0007337 exon 17540 17648 17540 ID=Msa1345670-mRNA-1:exon:5121;Parent=Msa1345670-mRNA-1
tig0007337 exon 19302 19609 19302 ID=Msa1345670-mRNA-1:exon:5120;Parent=Msa1345670-mRNA-1
tig0007337 exon 19639 19671 19639 ID=Msa1345670-mRNA-1:exon:5119;Parent=Msa1345670-mRNA-1
tig0007337 five_prime_UTR 19639 19671 19639 ID=Msa1345670-mRNA-1:five_prime_utr;Parent=Msa1345670-mRNA-1
tig0007337 five_prime_UTR 19373 19609 19373 ID=Msa1345670-mRNA-1:five_prime_utr;Parent=Msa1345670-mRNA-1
tig0007337 CDS 19302 19372 19302 ID=Msa1345670-mRNA-1:cds;Parent=Msa1345670-mRNA-1
tig0007337 CDS 17540 17648 17540 ID=Msa1345670-mRNA-1:cds;Parent=Msa1345670-mRNA-1
tig0007337 CDS 16340 16423 16340 ID=Msa1345670-mRNA-1:cds;Parent=Msa1345670-mRNA-1
tig0007337 CDS 15516 15770 15516 ID=Msa1345670-mRNA-1:cds;Parent=Msa1345670-mRNA-1
tig0007337 three_prime_UTR 15199 15515 15199 ID=Msa1345670-mRNA-1:three_prime_utr;Parent=Msa1345670-mRNA-1
Gene Sequence

>Msa1345670

ATGTCTGATTTGGTTGCTCGTACGGGTCGGCATCAGCAACGCTATGAAGATGGTTATCGTCTTGTTGCTGGGTGTGTTCCCTTTAGGTATAAAAGCTGTGAAGACGACTCTAGTTCCGAGAAGATTGTTGAAGTGCTTATGATTAATTCACCTAGTGGACCAGGTCTTTTGTTTCCAAAGGGAGGTTGGGAGAATGATGAAACTGTTGAGGAGGCGGCTGTAAGAGAAGCTATAGAAGAAGCGGGAGTTCGAGGAGACCTAATGGAATGTCTTGGATACTATGAGTTTAGGAGTAAGACCCATCAAGATGAGTTCAGTCCAGAAGGGTTGTGTAAAGCTGCAATGTTTGCTTTGTTTGTCAAGGAGGAACTTGAACTATGGCCTGAGCAAAATACCAGAAATAGGAGTTGGCTGGATGTATCGGAGGCACTTGATAGCCTCCGACATGAGTGGATGAGGGACGCTCTGGAACGCTTCTGCAAATGGCATGAGGAAAAGTTTGTGGATGGAACAGAATGA
Protein sequence

>Msa1345670

MSDLVARTGRHQQRYEDGYRLVAGCVPFRYKSCEDDSSSEKIVEVLMINSPSGPGLLFPKGGWENDETVEEAAVREAIEEAGVRGDLMECLGYYEFRSKTHQDEFSPEGLCKAAMFALFVKEELELWPEQNTRNRSWLDVSEALDSLRHEWMRDALERFCKWHEEKFVDGTE*