AlfalfaGEDB | Msa1348620

M. sativa cultivar zhongmu-4 / Msa1348620

KEGG: K14488
GO: -
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1348620	XP_003603566.1	98.830	171	2	0	1	171	1	171	2.00e-122	352

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1348620	sp\|Q9LTV3\|SAU72_ARATH	33.913	115	72	2	16	130	1	111	2.66e-17	77.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1348620	G7J2U8	98.830	171	2	0	1	171	1	171	9.55e-123	352

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010150	Msa1348620	0.803675	3.112646e-49	-8.615850e-47
Msa1348620	Msa1355340	0.814679	1.362659e-51	-8.615850e-47
Msa1348620	Msa1455360	0.807257	5.522063e-50	-8.615850e-47
Msa0606600	Msa1348620	0.820819	5.607101e-53	-8.615850e-47
Msa0629160	Msa1348620	0.803991	2.676354e-49	-8.615850e-47
Msa0631700	Msa1348620	0.802358	5.827864e-49	-8.615850e-47
Msa0647300	Msa1348620	0.840533	8.211983e-58	-8.615850e-47
Msa0679220	Msa1348620	0.812300	4.543886e-51	-8.615850e-47
Msa0686830	Msa1348620	0.843130	1.692670e-58	-8.615850e-47
Msa0699170	Msa1348620	0.808280	3.346997e-50	-8.615850e-47
Msa0766170	Msa1348620	0.800208	1.605439e-48	-8.615850e-47
Msa0799110	Msa1348620	0.813234	2.837341e-51	-8.615850e-47
Msa0799120	Msa1348620	0.809799	1.582642e-50	-8.615850e-47
Msa0207640	Msa1348620	0.802308	5.968135e-49	-8.615850e-47
Msa1225850	Msa1348620	0.802431	5.627645e-49	-8.615850e-47
Msa1238250	Msa1348620	0.800931	1.143143e-48	-8.615850e-47
Msa1263110	Msa1348620	0.804185	2.438365e-49	-8.615850e-47
Msa1311370	Msa1348620	0.811795	5.854478e-51	-8.615850e-47
Msa1316960	Msa1348620	0.820674	6.052894e-53	-8.615850e-47
Msa1319030	Msa1348620	0.814900	1.217120e-51	-8.615850e-47
Msa1322500	Msa1348620	0.813458	2.533256e-51	-8.615850e-47
Msa0433550	Msa1348620	0.972291	1.582072e-134	-8.615850e-47
Msa0464310	Msa1348620	0.814158	1.776059e-51	-8.615850e-47
Msa1030320	Msa1348620	0.818264	2.146460e-52	-8.615850e-47
Msa1069350	Msa1348620	0.805978	1.028136e-49	-8.615850e-47
Msa1075090	Msa1348620	0.816256	6.074592e-52	-8.615850e-47
Msa1078120	Msa1348620	-0.808182	3.511895e-50	-8.615850e-47
Msa1100330	Msa1348620	0.801872	7.334172e-49	-8.615850e-47
Msa0265650	Msa1348620	0.830276	3.217731e-55	-8.615850e-47
Msa0279810	Msa1348620	0.803747	3.007930e-49	-8.615850e-47
Msa0307040	Msa1348620	0.807973	3.889829e-50	-8.615850e-47
Msa1108560	Msa1348620	0.805323	1.411174e-49	-8.615850e-47
Msa1147010	Msa1348620	0.807932	3.969070e-50	-8.615850e-47
Msa1156240	Msa1348620	0.803680	3.106137e-49	-8.615850e-47
Msa1157210	Msa1348620	0.830285	3.201528e-55	-8.615850e-47
Msa1165460	Msa1348620	0.813408	2.599293e-51	-8.615850e-47
Msa0490100	Msa1348620	0.804265	2.347291e-49	-8.615850e-47
Msa0501080	Msa1348620	0.803908	2.784326e-49	-8.615850e-47
Msa0535570	Msa1348620	0.806082	9.775840e-50	-8.615850e-47
Msa0867080	Msa1348620	0.819910	9.062860e-53	-8.615850e-47
Msa0970270	Msa1348620	0.805721	1.164271e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1348620	MtrunA17_Chr3g0139171	98.830	171	2	0	1	171	1	171	1.84e-126	352
Msa1348620	MtrunA17_Chr1g0147571	69.492	177	41	5	5	171	39	212	9.64e-79	233
Msa1348620	MtrunA17_Chr4g0000251	60.294	136	46	2	5	135	4	136	7.04e-45	145
Msa1348620	MtrunA17_Chr4g0009931	70.297	101	23	3	48	141	1	101	1.05e-40	132
Msa1348620	MtrunA17_Chr2g0301531	43.609	133	70	2	10	137	12	144	5.13e-30	107
Msa1348620	MtrunA17_Chr1g0156341	34.615	156	64	3	6	153	4	129	4.37e-23	89.4
Msa1348620	MtrunA17_Chr4g0070071	38.710	124	71	2	14	133	11	133	8.26e-23	89.0
Msa1348620	MtrunA17_Chr4g0036141	36.567	134	49	2	8	140	11	109	2.97e-18	76.6
Msa1348620	MtrunA17_Chr2g0311341	33.613	119	75	1	16	130	1	119	2.75e-17	74.3
Msa1348620	MtrunA17_Chr4g0036531	46.250	80	42	1	56	134	12	91	2.13e-16	70.5
Msa1348620	MtrunA17_Chr5g0396091	42.857	84	47	1	52	134	12	95	3.58e-16	70.1
Msa1348620	MtrunA17_Chr4g0036161	41.667	84	46	1	49	132	19	99	5.07e-16	69.7
Msa1348620	MtrunA17_Chr7g0214201	32.576	132	85	2	15	146	15	142	5.08e-16	71.2
Msa1348620	MtrunA17_Chr4g0036841	53.165	79	35	2	51	128	18	95	7.48e-16	69.3
Msa1348620	MtrunA17_Chr3g0121721	53.731	67	30	1	69	134	25	91	1.00e-15	68.6
Msa1348620	MtrunA17_Chr3g0131991	30.488	164	90	5	10	167	4	149	1.09e-15	70.1
Msa1348620	MtrunA17_Chr2g0281291	52.239	67	31	1	69	134	26	92	1.37e-15	68.6
Msa1348620	MtrunA17_Chr8g0358971	52.239	67	31	1	69	134	25	91	2.03e-15	67.8
Msa1348620	MtrunA17_Chr3g0145371	46.875	64	34	0	69	132	45	108	2.46e-15	68.2
Msa1348620	MtrunA17_Chr4g0036331	54.098	61	27	1	69	128	23	83	4.54e-15	67.0
Msa1348620	MtrunA17_Chr8g0358871	46.970	66	35	0	67	132	33	98	4.89e-15	67.8
Msa1348620	MtrunA17_Chr3g0142421	39.326	89	49	1	49	132	17	105	4.93e-15	67.4
Msa1348620	MtrunA17_Chr4g0036271	54.098	61	27	1	69	128	23	83	5.22e-15	66.6
Msa1348620	MtrunA17_Chr3g0121701	44.706	85	45	2	51	134	8	91	5.91e-15	66.6
Msa1348620	MtrunA17_Chr3g0121731	44.706	85	45	2	51	134	8	91	6.87e-15	66.6
Msa1348620	MtrunA17_Chr4g0036821	49.254	67	33	1	69	134	26	92	6.97e-15	66.6
Msa1348620	MtrunA17_Chr3g0127561	38.000	100	56	2	36	133	4	99	8.61e-15	67.0
Msa1348620	MtrunA17_Chr5g0405301	40.230	87	49	1	49	132	17	103	9.08e-15	66.6
Msa1348620	MtrunA17_Chr4g0036201	44.304	79	43	1	51	128	8	86	1.18e-14	65.9
Msa1348620	MtrunA17_Chr4g0036611	45.455	77	41	1	53	128	9	85	1.26e-14	65.9
Msa1348620	MtrunA17_Chr8g0346181	44.118	68	36	1	68	133	26	93	1.27e-14	66.2
Msa1348620	MtrunA17_Chr4g0036661	53.226	62	28	1	68	128	19	80	1.59e-14	65.5
Msa1348620	MtrunA17_Chr8g0358981	49.254	67	33	1	69	134	25	91	2.00e-14	65.5
Msa1348620	MtrunA17_Chr4g0036431	43.750	80	44	1	56	134	12	91	2.34e-14	65.1
Msa1348620	MtrunA17_Chr4g0036691	46.753	77	40	1	53	128	4	80	2.48e-14	64.7
Msa1348620	MtrunA17_Chr4g0036811	47.368	76	38	2	51	125	9	83	2.65e-14	65.1
Msa1348620	MtrunA17_Chr1g0178451	42.029	69	40	0	65	133	45	113	3.25e-14	65.9
Msa1348620	MtrunA17_Chr4g0036631	50.746	67	32	1	69	134	20	86	3.56e-14	64.7
Msa1348620	MtrunA17_Chr8g0389141	32.540	126	65	2	5	130	6	111	3.58e-14	66.2
Msa1348620	MtrunA17_Chr8g0359001	58.491	53	21	1	69	120	25	77	3.60e-14	64.3
Msa1348620	MtrunA17_Chr4g0036651	42.500	80	45	1	56	134	12	91	4.68e-14	64.3
Msa1348620	MtrunA17_Chr4g0036401	42.500	80	45	1	56	134	12	91	4.78e-14	64.3
Msa1348620	MtrunA17_Chr4g0036181	42.553	94	49	2	40	128	50	143	4.93e-14	65.9
Msa1348620	MtrunA17_Chr3g0139731	55.738	61	26	1	69	128	64	124	5.67e-14	65.5
Msa1348620	MtrunA17_Chr4g0036421	44.156	77	42	1	53	128	4	80	6.01e-14	63.9
Msa1348620	MtrunA17_Chr4g0036461	42.169	83	47	1	53	134	9	91	6.51e-14	63.9
Msa1348620	MtrunA17_Chr4g0036861	54.098	61	27	1	69	128	57	117	8.10e-14	64.7
Msa1348620	MtrunA17_Chr7g0276101	47.059	68	34	1	68	133	22	89	8.15e-14	63.9
Msa1348620	MtrunA17_Chr4g0036581	42.500	80	45	1	56	134	12	91	8.49e-14	63.5
Msa1348620	MtrunA17_Chr4g0036621	52.459	61	28	1	69	128	19	79	9.64e-14	63.5
Msa1348620	MtrunA17_Chr3g0121801	55.172	58	25	1	69	125	25	82	1.01e-13	63.5
Msa1348620	MtrunA17_Chr4g0036681	47.761	67	34	1	69	134	25	91	1.39e-13	63.2
Msa1348620	MtrunA17_Chr4g0036831	47.887	71	36	1	51	120	8	78	1.46e-13	63.2
Msa1348620	MtrunA17_Chr4g0036571	41.758	91	46	2	45	128	30	120	1.72e-13	63.9
Msa1348620	MtrunA17_Chr3g0139711	43.373	83	46	1	53	134	10	92	2.00e-13	62.8
Msa1348620	MtrunA17_Chr7g0266571	45.161	62	34	0	72	133	53	114	2.58e-13	63.5
Msa1348620	MtrunA17_Chr4g0036671	52.459	61	28	1	69	128	21	81	3.14e-13	62.0
Msa1348620	MtrunA17_Chr4g0036491	42.857	77	43	1	53	128	9	85	3.15e-13	62.0
Msa1348620	MtrunA17_Chr4g0036241	42.169	83	47	1	53	134	10	92	3.25e-13	62.4
Msa1348620	MtrunA17_Chr3g0139701	54.098	61	27	1	69	128	83	143	3.30e-13	63.5
Msa1348620	MtrunA17_Chr8g0359011	55.172	58	25	1	69	125	3	60	3.55e-13	61.6
Msa1348620	MtrunA17_Chr1g0179511	46.970	66	35	0	69	134	19	84	3.60e-13	62.8
Msa1348620	MtrunA17_Chr4g0036441	42.857	77	43	1	53	128	4	80	3.63e-13	62.0
Msa1348620	MtrunA17_Chr8g0389151	45.714	70	38	0	67	136	38	107	3.72e-13	63.5
Msa1348620	MtrunA17_Chr4g0036521	44.186	86	44	3	50	133	15	98	3.97e-13	62.0
Msa1348620	MtrunA17_Chr4g0036751	51.667	60	28	1	67	125	18	77	4.41e-13	61.6
Msa1348620	MtrunA17_Chr3g0121811	53.448	58	26	1	69	125	26	83	4.97e-13	61.6
Msa1348620	MtrunA17_Chr4g0036601	50.000	62	30	1	69	129	21	82	5.69e-13	61.2
Msa1348620	MtrunA17_Chr8g0372061	36.782	87	52	1	49	132	62	148	6.46e-13	62.8
Msa1348620	MtrunA17_Chr4g0036471	50.820	61	29	1	69	128	21	81	7.27e-13	61.2
Msa1348620	MtrunA17_Chr7g0266581	43.548	62	35	0	72	133	53	114	7.98e-13	62.0
Msa1348620	MtrunA17_Chr4g0036211	46.053	76	40	1	51	125	146	221	8.31e-13	64.3
Msa1348620	MtrunA17_Chr4g0036211	45.714	70	37	1	51	119	8	77	4.37e-11	59.7
Msa1348620	MtrunA17_Chr3g0145401	37.069	116	53	2	14	128	9	105	1.11e-12	61.2
Msa1348620	MtrunA17_Chr4g0036501	50.820	61	29	1	69	128	21	81	1.33e-12	60.5
Msa1348620	MtrunA17_Chr4g0036391	50.000	62	30	1	68	128	19	80	1.43e-12	60.5
Msa1348620	MtrunA17_Chr4g0036411	50.820	61	29	1	69	128	21	81	2.57e-12	59.7
Msa1348620	MtrunA17_Chr4g0036791	52.459	61	28	1	69	128	27	87	2.66e-12	59.7
Msa1348620	MtrunA17_Chr4g0036641	50.820	61	29	1	69	128	21	81	3.18e-12	59.3
Msa1348620	MtrunA17_Chr4g0036541	49.180	61	30	1	69	128	20	80	3.26e-12	59.3
Msa1348620	MtrunA17_Chr4g0036551	50.000	58	28	1	69	125	20	77	3.67e-12	59.3
Msa1348620	MtrunA17_Chr4g0011821	42.105	76	40	2	65	136	75	150	3.89e-12	61.2
Msa1348620	MtrunA17_Chr4g0036231	44.928	69	37	1	53	120	18	86	3.91e-12	59.7
Msa1348620	MtrunA17_Chr4g0036761	43.590	78	33	2	69	145	23	90	4.56e-12	59.3
Msa1348620	MtrunA17_Chr6g0481791	39.759	83	47	2	52	133	19	99	5.62e-12	59.3
Msa1348620	MtrunA17_Chr4g0036191	52.459	61	26	2	69	128	26	84	8.11e-12	58.5
Msa1348620	MtrunA17_Chr3g0121751	43.590	78	39	1	40	112	49	126	8.22e-12	60.1
Msa1348620	MtrunA17_Chr7g0230411	35.052	97	58	2	44	137	52	146	1.06e-11	59.7
Msa1348620	MtrunA17_Chr4g0036801	49.254	67	33	1	69	134	26	92	1.07e-11	58.2
Msa1348620	MtrunA17_Chr4g0036561	50.820	61	29	1	69	128	20	80	1.34e-11	57.8
Msa1348620	MtrunA17_Chr5g0396071	41.250	80	46	1	56	134	8	87	1.52e-11	57.8
Msa1348620	MtrunA17_Chr4g0000241	38.824	85	45	2	52	129	40	124	2.38e-11	58.9
Msa1348620	MtrunA17_Chr7g0264571	42.105	76	42	1	57	130	23	98	2.77e-11	57.8
Msa1348620	MtrunA17_Chr1g0199531	50.820	61	30	0	69	129	50	110	2.78e-11	57.8
Msa1348620	MtrunA17_Chr3g0121761	46.269	67	35	1	69	134	25	91	3.08e-11	57.0
Msa1348620	MtrunA17_Chr8g0358991	51.724	58	27	1	69	125	25	82	3.24e-11	57.0
Msa1348620	MtrunA17_Chr5g0396081	51.923	52	24	1	70	120	21	72	3.45e-11	56.6
Msa1348620	MtrunA17_Chr4g0036711	45.588	68	36	1	67	133	13	80	5.11e-11	56.2
Msa1348620	MtrunA17_Chr3g0121741	50.000	58	28	1	69	125	25	82	7.08e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1348620	AT4G31320.1	53.646	192	58	5	5	171	4	189	1.20e-56	176
Msa1348620	AT2G24400.1	54.011	187	54	6	5	171	4	178	7.99e-56	174
Msa1348620	AT3G43120.1	40.288	139	75	1	10	140	12	150	2.14e-29	106
Msa1348620	AT5G20810.2	44.697	132	65	3	10	133	12	143	1.59e-27	102
Msa1348620	AT5G20810.1	44.697	132	65	3	10	133	12	143	1.60e-27	101
Msa1348620	AT1G19840.1	37.795	127	50	1	6	132	7	104	5.13e-20	82.0
Msa1348620	AT5G10990.1	36.567	134	56	1	6	139	7	111	4.10e-19	79.3
Msa1348620	AT4G34750.2	36.220	127	52	2	6	132	3	100	1.20e-18	78.2
Msa1348620	AT4G34750.1	36.220	127	52	2	6	132	3	100	1.20e-18	78.2
Msa1348620	AT3G12830.1	33.913	115	72	2	16	130	1	111	2.70e-18	77.0
Msa1348620	AT1G56150.1	33.913	115	60	1	16	130	1	99	1.28e-17	74.3
Msa1348620	AT1G79130.1	35.593	118	64	2	16	130	1	109	2.28e-17	74.3
Msa1348620	AT1G75590.1	37.795	127	51	2	6	132	7	105	3.60e-17	74.7
Msa1348620	AT4G34760.1	39.326	89	49	1	49	132	18	106	4.32e-17	73.2
Msa1348620	AT4G36110.1	42.268	97	54	2	37	131	6	102	1.03e-16	72.0
Msa1348620	AT3G20220.1	40.404	99	57	2	32	129	11	108	4.53e-16	70.9
Msa1348620	AT3G61900.1	52.239	67	29	1	69	132	30	96	5.52e-16	70.9
Msa1348620	AT1G75580.1	35.156	128	59	3	5	132	4	107	3.46e-15	68.2
Msa1348620	AT2G16580.1	33.333	120	59	2	19	132	3	107	4.07e-15	67.8
Msa1348620	AT2G21210.1	48.193	83	35	3	46	125	10	87	9.81e-15	66.6
Msa1348620	AT5G66260.1	40.000	85	50	1	49	132	13	97	1.04e-14	66.6
Msa1348620	AT2G18010.1	46.032	63	34	0	69	131	48	110	2.21e-14	66.2
Msa1348620	AT2G21220.1	40.230	87	49	1	49	132	17	103	2.50e-14	65.9
Msa1348620	AT4G38860.1	39.806	103	56	3	34	132	4	104	2.65e-14	65.9
Msa1348620	AT2G21210.2	42.308	104	48	5	33	125	52	154	3.70e-14	67.0
Msa1348620	AT2G46690.1	42.857	70	37	1	69	135	24	93	8.89e-14	64.7
Msa1348620	AT1G19830.1	31.967	122	63	2	8	129	7	108	1.03e-13	64.7
Msa1348620	AT4G38840.1	55.932	59	24	2	69	125	33	91	1.07e-13	63.9
Msa1348620	AT3G60690.1	42.683	82	40	2	67	145	86	163	1.78e-13	65.1
Msa1348620	AT4G00880.1	47.887	71	35	1	65	133	24	94	1.99e-13	63.9
Msa1348620	AT4G34770.1	45.556	90	42	4	51	134	15	103	3.24e-13	62.8
Msa1348620	AT1G16510.1	30.833	120	77	2	16	132	1	117	6.00e-13	63.2
Msa1348620	AT3G51200.1	46.154	65	34	1	69	132	39	103	7.08e-12	59.3
Msa1348620	AT4G12410.1	42.667	75	40	1	65	136	72	146	9.24e-12	60.5
Msa1348620	AT5G53590.1	41.429	70	36	1	69	133	46	115	1.18e-11	59.7
Msa1348620	AT4G34800.1	49.231	65	29	2	69	129	21	85	1.19e-11	58.5
Msa1348620	AT3G03850.1	41.758	91	46	3	51	134	3	93	2.12e-11	57.8
Msa1348620	AT4G22620.1	43.056	72	38	1	68	136	78	149	3.06e-11	58.9
Msa1348620	AT5G18080.1	41.463	82	42	3	46	125	5	82	3.31e-11	57.4
Msa1348620	AT4G13790.1	42.647	68	36	2	68	132	24	91	3.78e-11	57.0
Msa1348620	AT4G38825.1	42.857	63	34	2	68	128	22	84	3.79e-11	57.0
Msa1348620	AT2G36210.1	31.200	125	75	4	11	133	4	119	9.27e-11	57.4

Find 34 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTATACCAACTGAGTATT+TGG	0.160089	tig0010471:+30571	Msa1348620:CDS
AGAGAAGCTGAAGAAGAATT+TGG	0.168030	tig0010471:+30620	Msa1348620:CDS
CTACTCTTCTCCATTTCTTA+AGG	0.303486	tig0010471:-30373	None:intergenic
GTGTTGGTGTAGACCTTAAC+AGG	0.314144	tig0010471:+30546	Msa1348620:CDS
TCTACTCAGAAATGTAGATT+TGG	0.343922	tig0010471:+30731	Msa1348620:CDS
GAGAAGCTGAAGAAGAATTT+GGG	0.377927	tig0010471:+30621	Msa1348620:CDS
TTCAACAGATCCTTAAGAAA+TGG	0.395955	tig0010471:+30363	Msa1348620:CDS
GAATTTGGGTTTGAACAAAC+TGG	0.403966	tig0010471:+30635	Msa1348620:CDS
TGATCCTCCTCCTTCCCGTT+CGG	0.422045	tig0010471:-30489	None:intergenic
TTGGTATAACAAACCTGTTA+AGG	0.460530	tig0010471:-30559	None:intergenic
ATTTGGCATTGAAAAGATGA+TGG	0.461164	tig0010471:+30748	Msa1348620:CDS
ATAATAACCAAGTTGGTTTG+AGG	0.468390	tig0010471:-30780	None:intergenic
AACACTTTCAATGTCCGAAC+GGG	0.473602	tig0010471:+30475	Msa1348620:CDS
CTTGAAGATGGTTGAAGGAA+AGG	0.483489	tig0010471:+30700	Msa1348620:CDS
TACTGTTCCTCAAACCAACT+TGG	0.509698	tig0010471:+30773	Msa1348620:CDS
GGCTACCTAGCCGTTTGTGT+TGG	0.517754	tig0010471:+30530	Msa1348620:CDS
GTTGATGATAATAACCAAGT+TGG	0.520248	tig0010471:-30787	None:intergenic
CAAACGGCTAGGTAGCCTTT+CGG	0.521166	tig0010471:-30524	None:intergenic
TATCTGCACATTGGACTATG+AGG	0.522020	tig0010471:-30809	None:intergenic
AGTATCTTGAAGATGGTTGA+AGG	0.527345	tig0010471:+30695	Msa1348620:CDS
GTTTGAGAGTATCTTGAAGA+TGG	0.543069	tig0010471:+30688	Msa1348620:CDS
AAGGTCTACACCAACACAAA+CGG	0.549240	tig0010471:-30540	None:intergenic
TTTGGCATTGAAAAGATGAT+GGG	0.560948	tig0010471:+30749	Msa1348620:CDS
ATGTCCGAACGGGAAGGAGG+AGG	0.563260	tig0010471:+30485	Msa1348620:CDS
TCAAACAACGCGGTTCCGAA+AGG	0.566250	tig0010471:+30509	Msa1348620:CDS
TTCTCTGAGTAATATATGAA+AGG	0.569528	tig0010471:-30603	None:intergenic
TGATGAGCCAAATACTCAGT+TGG	0.592407	tig0010471:-30578	None:intergenic
ATGACAAACTATCTGCACAT+TGG	0.610970	tig0010471:-30818	None:intergenic
GAACACTTTCAATGTCCGAA+CGG	0.618386	tig0010471:+30474	Msa1348620:CDS
TCAATGTCCGAACGGGAAGG+AGG	0.628171	tig0010471:+30482	Msa1348620:CDS
CTACACCAACACAAACGGCT+AGG	0.632675	tig0010471:-30535	None:intergenic
CTTTCAATGTCCGAACGGGA+AGG	0.652936	tig0010471:+30479	Msa1348620:CDS
TCAAACACTGAAACTTCACA+AGG	0.665855	tig0010471:-30671	None:intergenic
AGGAGGAGGATCAAACAACG+CGG	0.824181	tig0010471:+30499	Msa1348620:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAATTTCTCTGATTTTGTT+AGG	-	tig0010471:30338-30357	None:intergenic	25.0%
!!!	TCTGTTTGTTTTTGAAGTAT+TGG	-	tig0010471:30399-30418	None:intergenic	25.0%
!	TTCTCTGAGTAATATATGAA+AGG	-	tig0010471:30606-30625	None:intergenic	25.0%
!	ATTTTGTTAGGTTTCTTTGG+TGG	-	tig0010471:30326-30345	None:intergenic	30.0%
!	CTGATTTTGTTAGGTTTCTT+TGG	-	tig0010471:30329-30348	None:intergenic	30.0%
	TTCAACAGATCCTTAAGAAA+TGG	+	tig0010471:30363-30382	Msa1348620:CDS	30.0%
	TTGGTATAACAAACCTGTTA+AGG	-	tig0010471:30562-30581	None:intergenic	30.0%
	TGTTATACCAACTGAGTATT+TGG	+	tig0010471:30571-30590	Msa1348620:CDS	30.0%
	TCTACTCAGAAATGTAGATT+TGG	+	tig0010471:30731-30750	Msa1348620:CDS	30.0%
!	ATTTGGCATTGAAAAGATGA+TGG	+	tig0010471:30748-30767	Msa1348620:CDS	30.0%
!	TTTGGCATTGAAAAGATGAT+GGG	+	tig0010471:30749-30768	Msa1348620:CDS	30.0%
!!	ATAATAACCAAGTTGGTTTG+AGG	-	tig0010471:30783-30802	None:intergenic	30.0%
!!	GTTGATGATAATAACCAAGT+TGG	-	tig0010471:30790-30809	None:intergenic	30.0%
	CTACTCTTCTCCATTTCTTA+AGG	-	tig0010471:30376-30395	None:intergenic	35.0%
	AGAGAAGCTGAAGAAGAATT+TGG	+	tig0010471:30620-30639	Msa1348620:CDS	35.0%
	GAGAAGCTGAAGAAGAATTT+GGG	+	tig0010471:30621-30640	Msa1348620:CDS	35.0%
!	GAATTTGGGTTTGAACAAAC+TGG	+	tig0010471:30635-30654	Msa1348620:CDS	35.0%
	TCAAACACTGAAACTTCACA+AGG	-	tig0010471:30674-30693	None:intergenic	35.0%
!!	GTTTGAGAGTATCTTGAAGA+TGG	+	tig0010471:30688-30707	Msa1348620:CDS	35.0%
	AGTATCTTGAAGATGGTTGA+AGG	+	tig0010471:30695-30714	Msa1348620:CDS	35.0%
	GAACACTTTCAATGTCCGAA+CGG	+	tig0010471:30474-30493	Msa1348620:CDS	40.0%
	AACACTTTCAATGTCCGAAC+GGG	+	tig0010471:30475-30494	Msa1348620:CDS	40.0%
	AAGGTCTACACCAACACAAA+CGG	-	tig0010471:30543-30562	None:intergenic	40.0%
	TGATGAGCCAAATACTCAGT+TGG	-	tig0010471:30581-30600	None:intergenic	40.0%
	CTTGAAGATGGTTGAAGGAA+AGG	+	tig0010471:30700-30719	Msa1348620:CDS	40.0%
	TACTGTTCCTCAAACCAACT+TGG	+	tig0010471:30773-30792	Msa1348620:CDS	40.0%
	TATCTGCACATTGGACTATG+AGG	-	tig0010471:30812-30831	None:intergenic	40.0%
!	GTGTTGGTGTAGACCTTAAC+AGG	+	tig0010471:30546-30565	Msa1348620:CDS	45.0%
	CTTTCAATGTCCGAACGGGA+AGG	+	tig0010471:30479-30498	Msa1348620:CDS	50.0%
	AGGAGGAGGATCAAACAACG+CGG	+	tig0010471:30499-30518	Msa1348620:CDS	50.0%
	TCAAACAACGCGGTTCCGAA+AGG	+	tig0010471:30509-30528	Msa1348620:CDS	50.0%
	CAAACGGCTAGGTAGCCTTT+CGG	-	tig0010471:30527-30546	None:intergenic	50.0%
	CTACACCAACACAAACGGCT+AGG	-	tig0010471:30538-30557	None:intergenic	50.0%
	TCAATGTCCGAACGGGAAGG+AGG	+	tig0010471:30482-30501	Msa1348620:CDS	55.0%
	TGATCCTCCTCCTTCCCGTT+CGG	-	tig0010471:30492-30511	None:intergenic	55.0%
	GGCTACCTAGCCGTTTGTGT+TGG	+	tig0010471:30530-30549	Msa1348620:CDS	55.0%
	ATGTCCGAACGGGAAGGAGG+AGG	+	tig0010471:30485-30504	Msa1348620:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0010471	gene	30317	30832	30317	ID=Msa1348620;Name=Msa1348620
tig0010471	mRNA	30317	30832	30317	ID=Msa1348620-mRNA-1;Parent=Msa1348620;Name=Msa1348620-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|171
tig0010471	exon	30317	30832	30317	ID=Msa1348620-mRNA-1:exon:6491;Parent=Msa1348620-mRNA-1
tig0010471	CDS	30317	30832	30317	ID=Msa1348620-mRNA-1:cds;Parent=Msa1348620-mRNA-1

Gene Sequence

>Msa1348620

ATGGATCCACCAAAGAAACCTAACAAAATCAGAGAAATTGTTAGACTTCAACAGATCCTTAAGAAATGGAGAAGAGTAGCCAATACTTCAAAAACAAACAGAAGCAGCAGCAACAATAACAACAGTACTACTAGTAAAAGCATAAAGTTTCTGAAAAGAACACTTTCAATGTCCGAACGGGAAGGAGGAGGATCAAACAACGCGGTTCCGAAAGGCTACCTAGCCGTTTGTGTTGGTGTAGACCTTAACAGGTTTGTTATACCAACTGAGTATTTGGCTCATCAAGCCTTTCATATATTACTCAGAGAAGCTGAAGAAGAATTTGGGTTTGAACAAACTGGTGTTCTGAGAATTCCTTGTGAAGTTTCAGTGTTTGAGAGTATCTTGAAGATGGTTGAAGGAAAGGATAGATTTTCTACTCAGAAATGTAGATTTGGCATTGAAAAGATGATGGGATACTGTTCCTCAAACCAACTTGGTTATTATCATCAACCTCATAGTCCAATGTGCAGATAG

Protein sequence

>Msa1348620

MDPPKKPNKIREIVRLQQILKKWRRVANTSKTNRSSSNNNNSTTSKSIKFLKRTLSMSEREGGGSNNAVPKGYLAVCVGVDLNRFVIPTEYLAHQAFHILLREAEEEFGFEQTGVLRIPCEVSVFESILKMVEGKDRFSTQKCRFGIEKMMGYCSSNQLGYYHQPHSPMCR*