AlfalfaGEDB | Msa1418500

M. sativa cultivar zhongmu-4 / Msa1418500

KEGG: K07897
GO: -
PFAM: PF00071.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418500	sp\|Q41640\|RAB7_VIGAC	88.940	217	12	3	1	216	1	206	1.27e-139	391

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418500	A0A072VQC0	94.907	216	2	1	1	216	1	207	1.04e-148	421

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0016540	Msa1418500	0.807896	4.039681e-50	-8.615850e-47
Msa0032020	Msa1418500	0.837395	5.330087e-57	-8.615850e-47
Msa0037410	Msa1418500	0.901444	2.663927e-78	-8.615850e-47
Msa0062350	Msa1418500	0.813138	2.978633e-51	-8.615850e-47
Msa0078360	Msa1418500	0.807666	4.522028e-50	-8.615850e-47
Msa0142410	Msa1418500	0.823681	1.215100e-53	-8.615850e-47
Msa0168090	Msa1418500	0.814098	1.831625e-51	-8.615850e-47
Msa0178120	Msa1418500	0.900245	8.886853e-78	-8.615850e-47
Msa0196190	Msa1418500	0.804961	1.680374e-49	-8.615850e-47
Msa0209140	Msa1418500	0.813141	2.973754e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418500	MtrunA17_Chr1g0201121	94.907	216	2	1	1	216	1	207	2.01e-152	421
Msa1418500	MtrunA17_Chr6g0466301	85.714	217	19	3	1	216	1	206	4.07e-134	375
Msa1418500	MtrunA17_Chr6g0465491	85.714	217	19	3	1	216	1	206	4.07e-134	375
Msa1418500	MtrunA17_Chr8g0383261	70.698	215	52	2	2	216	6	209	9.74e-112	318
Msa1418500	MtrunA17_Chr2g0331691	71.429	210	44	2	4	213	6	199	2.68e-108	309
Msa1418500	MtrunA17_Chr4g0004661	69.907	216	56	1	1	216	57	263	3.08e-108	311
Msa1418500	MtrunA17_Chr4g0034871	69.266	218	53	3	1	216	1	206	2.15e-105	302
Msa1418500	MtrunA17_Chr6g0466161	69.780	182	53	1	35	216	8	187	2.61e-87	256
Msa1418500	MtrunA17_Chr6g0465471	69.231	182	54	1	35	216	8	187	1.28e-86	254
Msa1418500	MtrunA17_Chr6g0466431	61.345	119	28	1	1	119	1	101	1.11e-47	152

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418500	AT4G09720.1	79.167	216	35	2	1	216	1	206	8.17e-126	354
Msa1418500	AT1G22740.1	79.167	216	32	2	1	216	1	203	9.04e-123	346
Msa1418500	AT4G09720.4	77.376	221	35	3	1	216	1	211	3.87e-122	345
Msa1418500	AT4G09720.3	75.330	227	35	3	1	216	1	217	4.94e-122	345
Msa1418500	AT1G52280.1	70.183	218	51	3	1	216	1	206	1.30e-107	308
Msa1418500	AT3G16100.1	68.807	218	54	3	1	216	1	206	3.47e-106	304
Msa1418500	AT4G09720.6	81.395	172	31	1	45	216	2	172	5.23e-104	298
Msa1418500	AT4G09720.5	81.395	172	31	1	45	216	2	172	5.23e-104	298
Msa1418500	AT4G09720.2	81.395	172	31	1	45	216	2	172	5.23e-104	298
Msa1418500	AT3G18820.1	68.349	218	55	3	1	216	1	206	8.96e-104	298

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Find 83 sgRNAs with CRISPR-Local

Find 358 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGCAACTCCAAGACTT+TGG	0.454601	tig0024604:-31708	None:intergenic
AAAAGTGAGTAGTTTATATA+TGG	0.396519	tig0024604:-30543	None:intergenic
AAACAATAGTACTATCAAAA+AGG	0.261754	tig0024604:-35428	None:intergenic
AACAAACGGGTAAGAAACCA+AGG	0.625931	tig0024604:-31102	None:intergenic
AACAAGATTGATATTGATGG+CGG	0.609685	tig0024604:+32603	Msa1418500:CDS
AAGGACTGGTGTGCTTCAAA+AGG	0.438389	tig0024604:+32729	Msa1418500:CDS
AATGACATAGCTGGTTACGG+TGG	0.700311	tig0024604:-30893	None:intergenic
AATGGAAATGCCCTTGTATC+AGG	0.343477	tig0024604:-32567	None:intergenic
AATTTGAAACACAGCATTGA+AGG	0.485101	tig0024604:-35327	None:intergenic
ACAAGATTGATATTGATGGC+GGG	0.576782	tig0024604:+32604	Msa1418500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCACACTTAATTGTTCAC+GGG	+	tig0024604:31127-31146	Msa1418500:intron	30.0%
AACCTTAAAGAAGTCAAATC+AGG	-	tig0024604:31373-31392	None:intergenic	30.0%
TTATGGTTATGACGAGAAAA+AGG	+	tig0024604:31933-31952	Msa1418500:intron	30.0%
GACATTAAAATGAATGTAGC+TGG	+	tig0024604:31992-32011	Msa1418500:intron	30.0%
CTTACAAAAAGTCAGAATCT+TGG	+	tig0024604:32023-32042	Msa1418500:intron	30.0%
TCACAACCTGACTAATTTAT+AGG	-	tig0024604:32214-32233	None:intergenic	30.0%
GCATTTCCATTTATATTGCT+TGG	+	tig0024604:32579-32598	Msa1418500:CDS	30.0%
GGAAACAAGATTGATATTGA+TGG	+	tig0024604:32600-32619	Msa1418500:CDS	30.0%
AACAAGATTGATATTGATGG+CGG	+	tig0024604:32603-32622	Msa1418500:CDS	30.0%
ATTAAGGAAACTTAGAAGTG+AGG	-	tig0024604:32656-32675	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024604	gene	30544	35690	30544	ID=Msa1418500;Name=Msa1418500
tig0024604	mRNA	30544	35690	30544	ID=Msa1418500-mRNA-1;Parent=Msa1418500;Name=Msa1418500-mRNA-1;_AED=0.16;_eAED=0.16;_QI=364\|0.83\|0.85\|1\|1\|1\|7\|394\|216
tig0024604	exon	30544	30960	30544	ID=Msa1418500-mRNA-1:exon:398;Parent=Msa1418500-mRNA-1
tig0024604	exon	31064	31117	31064	ID=Msa1418500-mRNA-1:exon:399;Parent=Msa1418500-mRNA-1
tig0024604	exon	31468	31567	31468	ID=Msa1418500-mRNA-1:exon:400;Parent=Msa1418500-mRNA-1
tig0024604	exon	31679	31825	31679	ID=Msa1418500-mRNA-1:exon:401;Parent=Msa1418500-mRNA-1
tig0024604	exon	32558	32638	32558	ID=Msa1418500-mRNA-1:exon:402;Parent=Msa1418500-mRNA-1
tig0024604	exon	32711	32856	32711	ID=Msa1418500-mRNA-1:exon:403;Parent=Msa1418500-mRNA-1
tig0024604	exon	35227	35690	35227	ID=Msa1418500-mRNA-1:exon:404;Parent=Msa1418500-mRNA-1
tig0024604	five_prime_UTR	30544	30907	30544	ID=Msa1418500-mRNA-1:five_prime_utr;Parent=Msa1418500-mRNA-1
tig0024604	CDS	30908	30960	30908	ID=Msa1418500-mRNA-1:cds;Parent=Msa1418500-mRNA-1
tig0024604	CDS	31064	31117	31064	ID=Msa1418500-mRNA-1:cds;Parent=Msa1418500-mRNA-1
tig0024604	CDS	31468	31567	31468	ID=Msa1418500-mRNA-1:cds;Parent=Msa1418500-mRNA-1
tig0024604	CDS	31679	31825	31679	ID=Msa1418500-mRNA-1:cds;Parent=Msa1418500-mRNA-1
tig0024604	CDS	32558	32638	32558	ID=Msa1418500-mRNA-1:cds;Parent=Msa1418500-mRNA-1
tig0024604	CDS	32711	32856	32711	ID=Msa1418500-mRNA-1:cds;Parent=Msa1418500-mRNA-1
tig0024604	CDS	35227	35296	35227	ID=Msa1418500-mRNA-1:cds;Parent=Msa1418500-mRNA-1
tig0024604	three_prime_UTR	35297	35690	35297	ID=Msa1418500-mRNA-1:three_prime_utr;Parent=Msa1418500-mRNA-1

Gene Sequence

>Msa1418500

ATGTCATTGCGCAGACGAACCTTGCTCAAGGTCATTGTTCTCGGCGATAGCGGGGTTGGAAAAACCTCCTTGATGAATCAGTATCCTCGTTCCTTGGTTTCTTACCCATATGTGCACAACAAGTTTAGTCAGCAGTATAAGGCAACAATTGGTGCTGATTTTGTCACTAAAGAACTTCAAATTGACGACAGACTCGTCACACTACAAATATGGGACACTGCTGGACAAGAGAGATTCCAAAGTCTTGGAGTTGCGTTTTATAGAGGGGCGGATTGCTGTGTTCTTGCGTATGATGTGAACGTCATGAGGTCGTTTGATTCACTTGACAACTGGCACGAGGAGTTTCTCAAACAGGCAAACCCTCCTGATACAAGGGCATTTCCATTTATATTGCTTGGAAACAAGATTGATATTGATGGCGGGAATAGTCGAGTGGTTTCTGAGAAGAAAGCAAAGGACTGGTGTGCTTCAAAAGGGAATATTCCCTACTTTGAGACATCTGCAAAAGAGGATTACAATGTTGATGCTGCATTCTTGTGTATTGCAAAGACTGCCCTGGCCAATGAGCGTGAACAGGACATATATTTTCAAGGGATTCCTGAGGCGGTTCCAGAGAGTGAGCAAAGTGGTGGTGGTGGATGTGCATGCTGA

Protein sequence

>Msa1418500

MSLRRRTLLKVIVLGDSGVGKTSLMNQYPRSLVSYPYVHNKFSQQYKATIGADFVTKELQIDDRLVTLQIWDTAGQERFQSLGVAFYRGADCCVLAYDVNVMRSFDSLDNWHEEFLKQANPPDTRAFPFILLGNKIDIDGGNSRVVSEKKAKDWCASKGNIPYFETSAKEDYNVDAAFLCIAKTALANEREQDIYFQGIPEAVPESEQSGGGGCAC*