AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006564

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006564.t1 MTR_4g077900 98.117 478 9 0 88 565 1 478 0.0 960
MS.gene006564.t1 MTR_5g027480 79.537 562 107 2 4 565 2 555 0.0 915
MS.gene006564.t1 MTR_5g027500 79.094 574 101 3 4 565 2 568 0.0 897
MS.gene006564.t1 MTR_1g026310 45.138 545 288 5 28 565 20 560 3.82e-160 469
MS.gene006564.t1 MTR_8g039720 44.102 551 287 7 28 565 17 559 8.86e-155 455
MS.gene006564.t1 MTR_1g090737 44.964 556 276 12 28 565 27 570 9.76e-142 422
MS.gene006564.t1 MTR_4g057730 41.651 533 295 8 33 557 10 534 6.83e-134 401
MS.gene006564.t1 MTR_3g088880 41.600 500 273 6 62 557 64 548 1.98e-130 392
MS.gene006564.t1 MTR_3g088870 43.173 498 263 9 66 557 67 550 2.35e-130 392
MS.gene006564.t1 MTR_1g063120 40.619 549 301 9 26 557 4 544 6.99e-130 391
MS.gene006564.t1 MTR_2g095510 39.706 544 303 8 33 565 10 539 4.45e-129 388
MS.gene006564.t1 MTR_1g063110 39.963 543 309 7 26 557 4 540 1.39e-125 380
MS.gene006564.t1 MTR_2g105570 40.828 507 288 8 66 563 44 547 2.42e-114 351
MS.gene006564.t1 MTR_1g073180 39.575 518 274 11 54 557 34 526 4.66e-111 342
MS.gene006564.t1 MTR_4g057730 41.667 468 257 8 33 492 10 469 2.46e-110 338
MS.gene006564.t1 MTR_4g128337 37.050 556 329 13 21 565 3 548 1.23e-104 326
MS.gene006564.t1 MTR_4g005750 38.418 531 313 9 38 563 19 540 3.26e-103 322
MS.gene006564.t1 MTR_1g073180 38.934 488 264 10 62 540 69 531 9.11e-101 315
MS.gene006564.t1 MTR_5g007640 38.690 504 298 7 66 563 87 585 9.28e-101 317
MS.gene006564.t1 MTR_4g057730 40.355 451 249 8 33 473 10 450 1.32e-100 312
MS.gene006564.t1 MTR_3g460770 34.186 547 338 8 28 565 8 541 5.29e-100 314
MS.gene006564.t1 MTR_2g095510 38.242 455 256 8 33 476 10 450 3.97e-95 298
MS.gene006564.t1 MTR_1g026470 39.637 386 222 5 28 406 20 401 1.97e-84 269
MS.gene006564.t1 MTR_1g026430 39.637 386 222 5 28 406 20 401 4.47e-84 268
MS.gene006564.t1 MTR_3g035130 31.936 501 293 16 76 555 32 505 1.29e-54 193
MS.gene006564.t1 MTR_5g076240 29.114 553 335 17 43 559 11 542 2.44e-46 171
MS.gene006564.t1 MTR_1569s0010 27.799 518 328 12 76 558 22 528 6.90e-46 169
MS.gene006564.t1 MTR_3g035130 31.648 455 263 16 76 509 32 459 5.98e-43 160
MS.gene006564.t1 MTR_3g064420 27.477 535 317 16 72 558 36 547 4.58e-41 157
MS.gene006564.t1 MTR_5g076220 28.190 525 323 16 72 559 36 543 2.00e-40 154
MS.gene006564.t1 MTR_0161s0050 29.223 373 228 9 206 558 188 544 7.51e-39 150
MS.gene006564.t1 MTR_0803s0020 28.458 506 296 16 78 551 263 734 9.38e-39 152
MS.gene006564.t1 MTR_4g011790 28.389 391 247 10 198 564 217 598 3.36e-38 149
MS.gene006564.t1 MTR_4g013210 29.208 404 243 12 194 564 52 445 6.81e-38 145
MS.gene006564.t1 MTR_8g074040 28.627 517 312 19 88 565 94 592 7.77e-37 145
MS.gene006564.t1 MTR_4g011790 28.496 379 238 10 198 552 217 586 2.71e-36 143
MS.gene006564.t1 MTR_4g011790 27.750 400 247 12 198 564 217 607 6.76e-36 142
MS.gene006564.t1 MTR_5g076200 26.908 524 329 16 72 558 36 542 1.95e-35 140
MS.gene006564.t1 MTR_5g008820 28.119 505 304 18 88 558 52 531 2.42e-34 137
MS.gene006564.t1 MTR_2g044180 30.707 368 216 11 214 558 110 461 6.60e-34 134
MS.gene006564.t1 MTR_2g044180 30.707 368 216 11 214 558 201 552 1.64e-33 135
MS.gene006564.t1 MTR_8g074040 29.534 386 229 14 206 565 106 474 4.40e-33 132
MS.gene006564.t1 MTR_2g030240 25.830 542 308 15 79 558 31 540 1.11e-32 132
MS.gene006564.t1 MTR_5g008800 27.400 500 318 14 88 559 52 534 1.96e-32 131
MS.gene006564.t1 MTR_5g023730 26.112 517 330 17 77 563 48 542 2.11e-32 131
MS.gene006564.t1 MTR_8g074120 28.191 376 223 12 208 559 137 489 3.68e-31 127
MS.gene006564.t1 MTR_4g013210 29.223 373 221 12 194 533 204 566 1.06e-30 126
MS.gene006564.t1 MTR_4g121600 25.237 527 328 16 77 558 47 552 4.22e-30 124
MS.gene006564.t1 MTR_4g013210 26.904 394 224 10 194 564 52 404 1.14e-29 121
MS.gene006564.t1 MTR_4g013210 26.904 394 224 10 194 564 204 556 3.16e-29 122
MS.gene006564.t1 MTR_1g026350 41.791 134 78 0 28 161 20 153 2.35e-28 111
MS.gene006564.t1 MTR_2g030240 26.562 384 231 9 212 558 77 446 2.57e-28 118
MS.gene006564.t1 MTR_1g104810 24.906 530 306 14 29 482 55 568 4.00e-26 113
MS.gene006564.t1 MTR_3g087980 26.543 486 269 18 76 485 106 579 1.68e-25 111
MS.gene006564.t1 MTR_1g104810 28.272 382 212 12 148 482 135 501 2.10e-24 107
MS.gene006564.t1 MTR_7g084850 23.529 595 400 17 10 565 146 724 7.14e-24 106
MS.gene006564.t1 MTR_7g109830 26.255 518 306 18 45 492 36 547 1.54e-23 105
MS.gene006564.t1 MTR_1g030290 29.912 341 171 14 208 487 250 583 2.32e-23 105
MS.gene006564.t1 MTR_3g084450 25.739 575 312 20 65 533 94 659 5.14e-22 100
MS.gene006564.t1 MTR_5g009350 24.860 535 313 19 30 487 22 544 9.16e-22 99.8
MS.gene006564.t1 MTR_5g009350 24.860 535 313 19 30 487 22 544 1.44e-21 99.0
MS.gene006564.t1 MTR_5g009350 24.860 535 313 19 30 487 22 544 1.60e-21 99.0
MS.gene006564.t1 MTR_5g009350 24.860 535 313 19 30 487 22 544 1.80e-21 99.0
MS.gene006564.t1 MTR_1g086600 24.517 518 317 14 45 492 58 571 2.50e-21 98.6
MS.gene006564.t1 MTR_5g009360 24.798 496 297 16 58 487 59 544 6.33e-21 97.4
MS.gene006564.t1 MTR_4g057755 52.778 72 34 0 421 492 46 117 8.70e-21 88.6
MS.gene006564.t1 MTR_8g099420 25.100 502 300 17 47 482 49 540 3.82e-20 94.7
MS.gene006564.t1 MTR_3g084450 26.517 445 232 16 170 533 21 451 4.60e-20 94.0
MS.gene006564.t1 MTR_5g009360 28.024 339 189 11 196 487 88 418 2.27e-19 92.0
MS.gene006564.t1 MTR_3g082660 28.107 338 165 14 210 481 284 609 1.71e-18 89.7
MS.gene006564.t1 MTR_1054s0020 23.307 502 339 12 73 556 294 767 2.13e-18 89.7
MS.gene006564.t1 MTR_8g083170 30.041 243 138 8 313 542 448 671 1.25e-17 87.0
MS.gene006564.t1 MTR_4g013210 26.821 302 167 9 194 482 204 464 6.85e-17 84.0
MS.gene006564.t1 MTR_3g084470 30.802 237 127 7 355 557 469 702 2.04e-16 83.2
MS.gene006564.t1 MTR_3g088890 50.794 63 31 0 467 529 10 72 1.37e-14 69.7
MS.gene006564.t1 MTR_2g098490 25.945 397 236 17 208 554 647 1035 1.83e-12 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006564.t1 AT4G19010 60.073 551 213 5 18 565 16 562 0.0 667
MS.gene006564.t1 AT1G20510 44.256 531 291 2 28 557 10 536 1.14e-163 477
MS.gene006564.t1 AT1G20480 41.894 549 298 7 28 565 27 565 5.72e-150 443
MS.gene006564.t1 AT1G20500 42.391 552 296 7 23 565 12 550 1.56e-149 441
MS.gene006564.t1 AT5G38120 40.901 555 299 9 23 565 13 550 5.60e-137 409
MS.gene006564.t1 AT4G05160 40.367 545 307 6 31 565 8 544 7.23e-136 406
MS.gene006564.t1 AT1G20510 42.918 466 261 2 28 492 10 471 5.63e-135 401
MS.gene006564.t1 AT5G63380 39.817 545 302 7 28 557 17 550 5.86e-135 404
MS.gene006564.t1 AT1G20510 41.480 446 256 2 28 472 10 451 1.59e-122 369
MS.gene006564.t1 AT5G38120 39.692 519 284 9 23 529 13 514 2.80e-121 367
MS.gene006564.t1 AT3G21240 40.445 539 296 11 38 565 30 554 1.04e-115 355
MS.gene006564.t1 AT5G38120 39.834 482 261 9 23 492 13 477 1.72e-110 338
MS.gene006564.t1 AT5G38120 39.834 482 261 9 23 492 13 477 1.72e-110 338
MS.gene006564.t1 AT1G51680 41.487 511 283 6 66 565 56 561 3.68e-110 340
MS.gene006564.t1 AT1G65060 39.020 551 304 11 20 558 27 557 2.54e-106 330
MS.gene006564.t1 AT3G21230 39.354 526 307 6 48 563 43 566 5.22e-105 327
MS.gene006564.t1 AT1G20490 39.394 429 240 8 23 442 12 429 6.98e-95 297
MS.gene006564.t1 AT1G20490 39.394 429 240 8 23 442 12 429 6.98e-95 297
MS.gene006564.t1 AT1G51680 40.421 475 267 6 66 529 56 525 2.92e-94 298
MS.gene006564.t1 AT1G62940 37.302 504 293 9 65 558 43 533 5.44e-94 298
MS.gene006564.t1 AT3G21230 38.538 506 295 7 48 543 43 542 1.74e-93 297
MS.gene006564.t1 AT3G21240 39.103 468 260 11 38 494 30 483 1.84e-88 282
MS.gene006564.t1 AT3G21240 39.103 468 260 11 38 494 30 483 1.84e-88 282
MS.gene006564.t1 AT1G51680 39.773 440 249 6 66 494 56 490 1.76e-83 269
MS.gene006564.t1 AT3G21230 38.293 457 270 6 48 494 43 497 3.75e-82 266
MS.gene006564.t1 AT1G65060 36.403 467 265 11 20 474 27 473 2.58e-73 242
MS.gene006564.t1 AT3G48990 28.866 485 331 9 75 555 30 504 6.28e-50 180
MS.gene006564.t1 AT5G16370 29.550 511 297 18 88 559 53 539 2.60e-46 171
MS.gene006564.t1 AT1G77240 30.171 527 304 19 75 563 43 543 3.37e-46 171
MS.gene006564.t1 AT3G21230 33.420 386 245 6 48 423 43 426 7.94e-46 167
MS.gene006564.t1 AT1G21540 27.405 551 343 19 49 563 19 548 2.16e-44 166
MS.gene006564.t1 AT1G21530 28.324 519 319 19 75 559 44 543 5.20e-43 162
MS.gene006564.t1 AT5G16340 29.883 512 292 18 88 558 53 538 6.49e-43 161
MS.gene006564.t1 AT1G65890 27.432 514 314 17 88 558 52 549 3.94e-42 160
MS.gene006564.t1 AT1G21530 28.240 517 320 19 75 559 44 541 8.96e-42 158
MS.gene006564.t1 AT1G65880 27.027 518 312 17 88 558 52 550 4.78e-40 154
MS.gene006564.t1 AT1G20560 29.026 503 308 15 88 558 54 539 3.78e-39 151
MS.gene006564.t1 AT1G75960 29.167 504 308 16 88 559 53 539 1.24e-38 149
MS.gene006564.t1 AT1G66120 28.373 504 315 15 88 558 52 542 3.74e-38 148
MS.gene006564.t1 AT1G30520 27.119 531 306 17 85 559 42 547 4.77e-36 142
MS.gene006564.t1 AT1G30520 27.119 531 306 17 85 559 42 547 4.77e-36 142
MS.gene006564.t1 AT1G20560 30.548 383 231 10 194 558 96 461 6.50e-35 137
MS.gene006564.t1 AT3G16910 29.847 392 234 10 191 558 181 555 2.98e-34 137
MS.gene006564.t1 AT2G17650 26.527 524 304 18 88 558 99 594 3.30e-34 137
MS.gene006564.t1 AT3G16170 27.577 388 237 12 207 564 234 607 1.13e-32 132
MS.gene006564.t1 AT1G76290 25.806 496 327 16 88 558 52 531 9.57e-30 123
MS.gene006564.t1 AT5G36880 25.094 530 353 15 74 565 196 719 1.18e-26 115
MS.gene006564.t1 AT5G36880 25.094 530 353 15 74 565 146 669 1.45e-26 115
MS.gene006564.t1 AT5G36880 25.094 530 353 15 74 565 146 669 1.45e-26 115
MS.gene006564.t1 AT5G36880 25.094 530 353 15 74 565 146 669 1.45e-26 115
MS.gene006564.t1 AT5G36880 25.094 530 353 15 74 565 146 669 1.45e-26 115
MS.gene006564.t1 AT5G27600 27.619 525 294 17 29 480 58 569 2.29e-26 114
MS.gene006564.t1 AT1G30520 26.622 447 258 16 85 489 42 460 3.18e-26 112
MS.gene006564.t1 AT1G30520 26.622 447 258 16 85 489 42 460 3.18e-26 112
MS.gene006564.t1 AT1G64400 24.952 521 308 16 34 480 27 538 6.71e-26 112
MS.gene006564.t1 AT1G68270 36.709 158 89 2 411 558 363 519 1.89e-24 107
MS.gene006564.t1 AT4G11030 23.722 489 298 15 59 480 58 538 5.89e-24 106
MS.gene006564.t1 AT1G49430 26.195 523 303 21 43 492 39 551 5.65e-23 103
MS.gene006564.t1 AT3G05970 30.699 329 170 11 199 480 252 569 1.56e-22 102
MS.gene006564.t1 AT1G77590 28.571 350 178 15 208 494 100 440 2.21e-21 98.2
MS.gene006564.t1 AT4G23850 23.608 521 315 19 34 480 27 538 4.29e-21 97.8
MS.gene006564.t1 AT1G77590 28.080 349 181 14 208 494 246 586 4.65e-21 97.8
MS.gene006564.t1 AT3G23790 32.919 161 93 4 355 503 474 631 5.53e-15 78.6
MS.gene006564.t1 AT4G14070 29.245 212 131 6 307 503 440 647 8.66e-11 65.5

Find 135 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTGCTTTACACAAAA+TGG 0.138296 4.4:-32673750 MS.gene006564:CDS
ACAGGGTAATAAATTGAATT+TGG 0.183276 4.4:+32673692 None:intergenic
CTCTTGGCTGTTCTGTATTT+AGG 0.185255 4.4:-32673665 MS.gene006564:CDS
GCCCTTATTGATTCTTCATC+TGG 0.217618 4.4:-32673818 MS.gene006564:CDS
TATCAAATACAAGGGATTTC+AGG 0.243292 4.4:-32671577 MS.gene006564:intron
GTTGAGTGATCATGTTGTTT+TGG 0.245364 4.4:+32673978 None:intergenic
TTGACATTGACGAGGTTATC+AGG 0.253484 4.4:-32673151 MS.gene006564:CDS
TCATAGAAATCTTATTTCTA+TGG 0.256021 4.4:-32673330 MS.gene006564:CDS
ACGGCTGCATCGACTATTTC+AGG 0.272026 4.4:+32671405 None:intergenic
AGTGCCGAAGATGAAGAAAT+TGG 0.290280 4.4:-32671245 MS.gene006564:CDS
TTTCACAACTATTCGTCAAT+AGG 0.295826 4.4:-32672332 MS.gene006564:CDS
GGGTCAGTTGGAAGGTTTCT+AGG 0.297530 4.4:+32673893 None:intergenic
ATGAAGGCAAAGGGTGTTAA+TGG 0.303488 4.4:-32673065 MS.gene006564:CDS
AGAACAGCCAAGAGTATAAC+AGG 0.326675 4.4:+32673674 None:intergenic
CAGGCAGCTGCGGTGAGCTT+TGG 0.328932 4.4:-32672247 MS.gene006564:CDS
CAAAGCTCACCGCAGCTGCC+TGG 0.332403 4.4:+32672248 None:intergenic
TTGCCAATGTTTCATGTGTA+TGG 0.342668 4.4:-32673236 MS.gene006564:CDS
ACCCAGATGAAGAATCAATA+AGG 0.350199 4.4:+32673816 None:intergenic
AGCAGTGCCTGAGTTGCTTT+CGG 0.356544 4.4:+32670937 None:intergenic
GCTGGTATATTGTATTCATC+TGG 0.361976 4.4:-32673386 MS.gene006564:CDS
TTCCCCAATTTCTTCATCTT+CGG 0.365786 4.4:+32671241 None:intergenic
TTCTTACCTCTCATAATAGA+AGG 0.374837 4.4:+32672215 None:intergenic
TTCACAACTATTCGTCAATA+GGG 0.376437 4.4:-32672331 MS.gene006564:CDS
ACCTGTATAAAATCAACGTT+AGG 0.376743 4.4:+32672957 None:intergenic
TGTTGGTGAAATTCGTAAAC+AGG 0.387052 4.4:-32673609 MS.gene006564:CDS
AAAGCTCACCGCAGCTGCCT+GGG 0.391998 4.4:+32672249 None:intergenic
TTATTACCCTGTTATACTCT+TGG 0.395992 4.4:-32673681 MS.gene006564:CDS
GTGCCGAAGATGAAGAAATT+GGG 0.397388 4.4:-32671244 MS.gene006564:CDS
TGCTGCGGGATTATTGTCGT+TGG 0.401502 4.4:-32673201 MS.gene006564:CDS
ACCTTCCGGACCTTCTTGTA+TGG 0.403187 4.4:+32671005 None:intergenic
TGTTGTTCCACCGATGTTGT+CGG 0.411176 4.4:-32673096 MS.gene006564:CDS
AAAGAAACAATGTCAAGAAA+AGG 0.413052 4.4:+32673872 None:intergenic
CCCTTATTGATTCTTCATCT+GGG 0.414327 4.4:-32673817 MS.gene006564:CDS
TGCTTGAGTTCTAAACTCTA+AGG 0.416696 4.4:-32670918 None:intergenic
TCAGGATGGATATTTACATT+TGG 0.418131 4.4:-32671619 MS.gene006564:CDS
GTCAAGAAACTAGAGCTATT+AGG 0.418468 4.4:-32673542 MS.gene006564:CDS
TCAAAGGGTAGTTATTAAAC+AGG 0.419883 4.4:-32673417 MS.gene006564:CDS
CTATTCGTCAATAGGGTTGT+TGG 0.424322 4.4:-32672324 MS.gene006564:CDS
GACAACATCGGTGGAACAAC+AGG 0.427365 4.4:+32673098 None:intergenic
TGTTTAATAACTACCCTTTG+AGG 0.430394 4.4:+32673419 None:intergenic
GGTTTATCGCTCTTTGCTGC+GGG 0.430597 4.4:-32673215 MS.gene006564:CDS
TAACAACTTTGAGTAAGAAA+TGG 0.436153 4.4:+32673790 None:intergenic
CAGAAAATGAGAAGGATTTG+AGG 0.445513 4.4:-32673493 MS.gene006564:CDS
GCTGCGGGATTATTGTCGTT+GGG 0.447735 4.4:-32673200 MS.gene006564:CDS
TGCGGTGAGCTTTGGTTGAG+AGG 0.454034 4.4:-32672239 MS.gene006564:CDS
GCTTCTGCTTTACACAAAAT+GGG 0.455667 4.4:-32673749 MS.gene006564:CDS
ACCCTCACCCTCACCCTCAT+TGG 0.457518 4.4:-32673955 MS.gene006564:CDS
CCCAGATGAAGAATCAATAA+GGG 0.467167 4.4:+32673817 None:intergenic
AATCAAATTTACCATAAAGA+AGG 0.470429 4.4:+32673444 None:intergenic
GCATTTGTTGTCAAGAAGGC+TGG 0.471765 4.4:-32671209 MS.gene006564:CDS
ATTATTGTCGTTGGGCTCTA+CGG 0.478036 4.4:-32673192 MS.gene006564:CDS
TACCAATGAGGGTGAGGGTG+AGG 0.484991 4.4:+32673953 None:intergenic
TAAATTTGATTTGCCTCAAA+GGG 0.485047 4.4:-32673432 MS.gene006564:CDS
GTAAATTTGATTTGCCTCAA+AGG 0.487083 4.4:-32673433 MS.gene006564:CDS
AACAGCTAGAAACACACTCT+TGG 0.487245 4.4:+32673259 None:intergenic
ACATAATCGACCACATGCTT+CGG 0.487322 4.4:+32671173 None:intergenic
TTGTATTCATCTGGGACTAC+AGG 0.489553 4.4:-32673377 MS.gene006564:CDS
ATTTCACCAACACTACTAAG+AGG 0.489624 4.4:+32673620 None:intergenic
CGGAGACCTTGGTATCTTGT+CGG 0.489928 4.4:+32670974 None:intergenic
AGTGGCATTTGTTGTCAAGA+AGG 0.496940 4.4:-32671213 MS.gene006564:CDS
AAGAAACAATGTCAAGAAAA+GGG 0.497596 4.4:+32673873 None:intergenic
AACACAGCTTCTAGATCAGC+AGG 0.500308 4.4:+32671438 None:intergenic
CACAAAATGGGTGTTTCACA+AGG 0.502968 4.4:-32673737 MS.gene006564:CDS
TTGAATCCTCTTAGTAGTGT+TGG 0.505325 4.4:-32673626 MS.gene006564:CDS
TTGAGCATAGGAGTTATTAG+TGG 0.506442 4.4:-32672996 MS.gene006564:CDS
ATCTTCCCAGTCGGAGACCT+TGG 0.507080 4.4:+32670963 None:intergenic
TGGTTTATCGCTCTTTGCTG+CGG 0.507976 4.4:-32673216 MS.gene006564:CDS
TGTCGGTGAAGAACACCTTC+CGG 0.510625 4.4:+32670991 None:intergenic
TAAGTTGCAGAGTTTGAGAC+AGG 0.511530 4.4:-32673039 MS.gene006564:CDS
TTGCTCCATACAAGAAGGTC+CGG 0.515213 4.4:-32671010 MS.gene006564:CDS
CGGAGGCAAAAGTGGTAGAC+TGG 0.515561 4.4:-32672292 MS.gene006564:CDS
AAAGATGGTTGGCTACATAC+AGG 0.515947 4.4:-32671660 MS.gene006564:CDS
GCGGTGAGCTTTGGTTGAGA+GGG 0.517348 4.4:-32672238 MS.gene006564:CDS
AAGCTCACCGCAGCTGCCTG+GGG 0.523366 4.4:+32672250 None:intergenic
CTGGTATATTGTATTCATCT+GGG 0.532782 4.4:-32673385 MS.gene006564:CDS
ATGTCAAGAAAAGGGTCAGT+TGG 0.533509 4.4:+32673881 None:intergenic
AAACCATACACATGAAACAT+TGG 0.533547 4.4:+32673233 None:intergenic
TCCAAACACGGAGGCAAAAG+TGG 0.534472 4.4:-32672300 MS.gene006564:CDS
TTCTTGACAACAAATGCCAC+TGG 0.535082 4.4:+32671215 None:intergenic
GAAGCCATGGATTTGACTAA+AGG 0.537715 4.4:+32673767 None:intergenic
ATATCAACAATTGATAAAGA+TGG 0.537763 4.4:-32671675 MS.gene006564:CDS
GTTACCTTTAGTCAAATCCA+TGG 0.539728 4.4:-32673771 MS.gene006564:CDS
AGATCCTCCGAAAGCAACTC+AGG 0.540928 4.4:-32670944 MS.gene006564:CDS
AAAGTGGTAGACTGGAACAA+TGG 0.541163 4.4:-32672284 MS.gene006564:CDS
CTCAGGATACTTGAATAATG+AGG 0.541417 4.4:-32671706 MS.gene006564:intron
TTCATATACTTACGCTGTAA+CGG 0.543086 4.4:+32671386 None:intergenic
CAAGAAAAGGGTCAGTTGGA+AGG 0.544290 4.4:+32673885 None:intergenic
AATCAACGTTAGGGAAAGCA+TGG 0.550452 4.4:+32672967 None:intergenic
GAAAGACATTATCAAATACA+AGG 0.550958 4.4:-32671586 MS.gene006564:CDS
GGTCGATTATGTTGCTGAGC+AGG 0.550976 4.4:-32671162 MS.gene006564:intron
GGGACTACAGGTGTGAGTAA+AGG 0.553349 4.4:-32673365 MS.gene006564:CDS
TTTGTGTAAAGCAGAAGCCA+TGG 0.560434 4.4:+32673754 None:intergenic
CCTGTATAAAATCAACGTTA+GGG 0.562718 4.4:+32672958 None:intergenic
TGACATTGACGAGGTTATCA+GGG 0.564262 4.4:-32673150 MS.gene006564:CDS
ACACTACTAAGAGGATTCAA+TGG 0.565007 4.4:+32673629 None:intergenic
CAACAATTGATAAAGATGGT+TGG 0.569594 4.4:-32671671 MS.gene006564:CDS
GGCATTGACAATGAAGGCAA+AGG 0.569984 4.4:-32673075 MS.gene006564:CDS
GCATTGACAATGAAGGCAAA+GGG 0.570042 4.4:-32673074 MS.gene006564:CDS
ATTTGATAATGTCTTTCAAG+CGG 0.579224 4.4:+32671592 None:intergenic
CTAATAACTCCTATGCTCAA+AGG 0.579398 4.4:+32672999 None:intergenic
ATTGTCAATGCCGACAACAT+CGG 0.579863 4.4:+32673086 None:intergenic
GGTGCAGCACCTTTGAGCAT+AGG 0.585948 4.4:-32673008 MS.gene006564:CDS
ACCAATGAGGGTGAGGGTGA+GGG 0.586326 4.4:+32673954 None:intergenic
TGCTGTGCCAGAAAATGAGA+AGG 0.586565 4.4:-32673501 MS.gene006564:CDS
AGTTTGAGACAGGTCTCTTG+CGG 0.592614 4.4:-32673029 MS.gene006564:CDS
GATGAATACCAATGAGGGTG+AGG 0.592922 4.4:+32673947 None:intergenic
GTTGTCGGCATTGACAATGA+AGG 0.595731 4.4:-32673081 MS.gene006564:CDS
AGGGCTAAAACCCCATCATG+AGG 0.601023 4.4:+32673836 None:intergenic
TGCCGAAGATGAAGAAATTG+GGG 0.602629 4.4:-32671243 MS.gene006564:CDS
AAGATACCAAGGTCTCCGAC+TGG 0.604082 4.4:-32670969 MS.gene006564:CDS
ATTAAACAGGAAGATACTGC+TGG 0.608838 4.4:-32673404 MS.gene006564:CDS
CGTAAACAGGTTCAAGAGTG+TGG 0.611754 4.4:-32673596 MS.gene006564:CDS
AGGGTTGTTGGCTCCAAACA+CGG 0.614077 4.4:-32672312 MS.gene006564:CDS
AAAGACATTATCAAATACAA+GGG 0.615550 4.4:-32671585 MS.gene006564:CDS
GTCAATGCCGACAACATCGG+TGG 0.615987 4.4:+32673089 None:intergenic
TTTCGGAGGATCTTCCCAGT+CGG 0.617027 4.4:+32670954 None:intergenic
AGAGGGCCTTCTATTATGAG+AGG 0.618979 4.4:-32672221 MS.gene006564:intron
AGATACCAAGGTCTCCGACT+GGG 0.620788 4.4:-32670968 MS.gene006564:CDS
GCAGGTTGCTCCATACAAGA+AGG 0.622353 4.4:-32671015 MS.gene006564:intron
TCGACTGCAGTGGGAACACG+TGG 0.624008 4.4:-32672371 MS.gene006564:CDS
GCTCTACGGTCATTGTAATG+AGG 0.634594 4.4:-32673178 MS.gene006564:CDS
AGCTCACCGCAGCTGCCTGG+GGG 0.641341 4.4:+32672251 None:intergenic
AGTGCCTGAGTTGCTTTCGG+AGG 0.646420 4.4:+32670940 None:intergenic
ATGACTGAGTCGACTGCAGT+GGG 0.649133 4.4:-32672380 MS.gene006564:CDS
GGGCTAAAACCCCATCATGA+GGG 0.653343 4.4:+32673837 None:intergenic
TCCATACAAGAAGGTCCGGA+AGG 0.659172 4.4:-32671006 MS.gene006564:CDS
TGTACTTTCTCCGAAGCATG+TGG 0.668913 4.4:-32671183 MS.gene006564:CDS
AATGACTGAGTCGACTGCAG+TGG 0.680151 4.4:-32672381 MS.gene006564:CDS
AAGAAACTAGAGCTATTAGG+TGG 0.680245 4.4:-32673539 MS.gene006564:CDS
GAGGAAATTTGACATTGACG+AGG 0.683873 4.4:-32673159 MS.gene006564:CDS
ATGAATACCAATGAGGGTGA+GGG 0.691317 4.4:+32673948 None:intergenic
AGAAATTGGGGAAATTCCAG+TGG 0.696561 4.4:-32671231 MS.gene006564:CDS
GAACAGCCAAGAGTATAACA+GGG 0.749221 4.4:+32673675 None:intergenic
TCTTCACCGACAAGATACCA+AGG 0.749835 4.4:-32670980 MS.gene006564:CDS
GTTGTTGGCTCCAAACACGG+AGG 0.795141 4.4:-32672309 MS.gene006564:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGGAAAAAAAAAATAAA+GGG + chr4.4:32673897-32673916 None:intergenic 15.0%
!!! GTTTGATTAACAATTTTAAT+TGG + chr4.4:32673193-32673212 None:intergenic 15.0%
!! AAAGACATTATCAAATACAA+GGG - chr4.4:32673348-32673367 MS.gene006564:CDS 20.0%
!! AATCAAATTTACCATAAAGA+AGG + chr4.4:32671492-32671511 None:intergenic 20.0%
!! ATATAGAGCAAATATAGTAA+AGG + chr4.4:32672875-32672894 None:intergenic 20.0%
!! ATATCAACAATTGATAAAGA+TGG - chr4.4:32673258-32673277 MS.gene006564:CDS 20.0%
!! ATTAAGACTATTCTATTCAA+AGG + chr4.4:32672008-32672027 None:intergenic 20.0%
!! CAGAAGGAAAAAAAAAATAA+AGG + chr4.4:32673898-32673917 None:intergenic 20.0%
!! TAGATATTAAAAGTGAAACA+CGG - chr4.4:32672758-32672777 MS.gene006564:intron 20.0%
!! TATAGAGCAAATATAGTAAA+GGG + chr4.4:32672874-32672893 None:intergenic 20.0%
!! TTAATAGCAACTGTTATATA+GGG - chr4.4:32672147-32672166 MS.gene006564:intron 20.0%
!! TTTAATAGCAACTGTTATAT+AGG - chr4.4:32672146-32672165 MS.gene006564:intron 20.0%
!!! AAATTTTATAAAGAAAAGCG+AGG - chr4.4:32673399-32673418 MS.gene006564:CDS 20.0%
!!! AGATATTGTTTATTTTGATC+AGG - chr4.4:32673296-32673315 MS.gene006564:CDS 20.0%
!!! AGTTTTTTTAACCTTCTTTA+TGG - chr4.4:32671478-32671497 MS.gene006564:intron 20.0%
!!! CAAATAAAGTTCAACTTTTA+TGG + chr4.4:32672464-32672483 None:intergenic 20.0%
!!! CTTTATTTTTTTTTTCCTTC+TGG - chr4.4:32673896-32673915 MS.gene006564:CDS 20.0%
!!! TCATAGAAATCTTATTTCTA+TGG - chr4.4:32671603-32671622 MS.gene006564:CDS 20.0%
! AAACTGAAGTAAATTTCTCT+TGG - chr4.4:32673075-32673094 MS.gene006564:CDS 25.0%
! AAAGAAACAATGTCAAGAAA+AGG + chr4.4:32671064-32671083 None:intergenic 25.0%
! AAATAAGCTGAAAAGAACTA+AGG - chr4.4:32672323-32672342 MS.gene006564:CDS 25.0%
! AACTAACATGATATTTCTCA+AGG + chr4.4:32672926-32672945 None:intergenic 25.0%
! AAGAAACAATGTCAAGAAAA+GGG + chr4.4:32671063-32671082 None:intergenic 25.0%
! AATAAATTGTTTAGCCAAAC+AGG + chr4.4:32672173-32672192 None:intergenic 25.0%
! ACAGGGTAATAAATTGAATT+TGG + chr4.4:32671244-32671263 None:intergenic 25.0%
! ATAGAGCAAATATAGTAAAG+GGG + chr4.4:32672873-32672892 None:intergenic 25.0%
! ATATAAAGGTCTTGATTCAA+TGG - chr4.4:32672786-32672805 MS.gene006564:intron 25.0%
! GAAAGACATTATCAAATACA+AGG - chr4.4:32673347-32673366 MS.gene006564:CDS 25.0%
! TAACAACTTTGAGTAAGAAA+TGG + chr4.4:32671146-32671165 None:intergenic 25.0%
! TTATAATCAAATGTCCAAAG+AGG + chr4.4:32673639-32673658 None:intergenic 25.0%
!! ATTTGTTAACGCTTAACTAA+AGG - chr4.4:32672893-32672912 MS.gene006564:intron 25.0%
!! TGTAAGATTGTTTCCTTTTA+GGG - chr4.4:32672523-32672542 MS.gene006564:intron 25.0%
!! TTTTTGATGAATACCAATGA+GGG + chr4.4:32670994-32671013 None:intergenic 25.0%
!!! ATTGTTTATTTTGATCAGGA+TGG - chr4.4:32673300-32673319 MS.gene006564:CDS 25.0%
!!! ATTTGATAATGTCTTTCAAG+CGG + chr4.4:32673344-32673363 None:intergenic 25.0%
!!! TAAATTTGATTTGCCTCAAA+GGG - chr4.4:32671501-32671520 MS.gene006564:intron 25.0%
AAACCATACACATGAAACAT+TGG + chr4.4:32671703-32671722 None:intergenic 30.0%
AACTCTTTCCTTTAATTCTC+AGG - chr4.4:32673210-32673229 MS.gene006564:CDS 30.0%
ACCTGTATAAAATCAACGTT+AGG + chr4.4:32671979-32671998 None:intergenic 30.0%
CAACAATTGATAAAGATGGT+TGG - chr4.4:32673262-32673281 MS.gene006564:CDS 30.0%
CATTGGTATTCATCAAAAAC+TGG - chr4.4:32670995-32671014 MS.gene006564:CDS 30.0%
CCTGTATAAAATCAACGTTA+GGG + chr4.4:32671978-32671997 None:intergenic 30.0%
CTGGTATATTGTATTCATCT+GGG - chr4.4:32671548-32671567 MS.gene006564:intron 30.0%
GAAAAATGACGTAACATTTG+AGG + chr4.4:32673662-32673681 None:intergenic 30.0%
GAAACACGGTAGTAATATAA+AGG - chr4.4:32672772-32672791 MS.gene006564:intron 30.0%
TATCAAATACAAGGGATTTC+AGG - chr4.4:32673356-32673375 MS.gene006564:CDS 30.0%
TCAAAGGGTAGTTATTAAAC+AGG - chr4.4:32671516-32671535 MS.gene006564:intron 30.0%
TCAAGCAATCATATAAACGA+TGG + chr4.4:32672051-32672070 None:intergenic 30.0%
TCAAGTATCCTGAGAATTAA+AGG + chr4.4:32673221-32673240 None:intergenic 30.0%
TCAGGATGGATATTTACATT+TGG - chr4.4:32673314-32673333 MS.gene006564:CDS 30.0%
TGTTTAATAACTACCCTTTG+AGG + chr4.4:32671517-32671536 None:intergenic 30.0%
TTATTACCCTGTTATACTCT+TGG - chr4.4:32671252-32671271 MS.gene006564:intron 30.0%
TTCACAACTATTCGTCAATA+GGG - chr4.4:32672602-32672621 MS.gene006564:intron 30.0%
TTCATATACTTACGCTGTAA+CGG + chr4.4:32673550-32673569 None:intergenic 30.0%
TTCTTACCTCTCATAATAGA+AGG + chr4.4:32672721-32672740 None:intergenic 30.0%
TTTCACAACTATTCGTCAAT+AGG - chr4.4:32672601-32672620 MS.gene006564:intron 30.0%
! CTGTAAGATTGTTTCCTTTT+AGG - chr4.4:32672522-32672541 MS.gene006564:intron 30.0%
! GTTTTTGATGAATACCAATG+AGG + chr4.4:32670995-32671014 None:intergenic 30.0%
!! GATTTCAGGTATCTACTTTT+AGG - chr4.4:32673370-32673389 MS.gene006564:CDS 30.0%
!! GTAAATTTGATTTGCCTCAA+AGG - chr4.4:32671500-32671519 MS.gene006564:intron 30.0%
!! TCTAGTTTCTTGACATTTTC+AGG + chr4.4:32671385-32671404 None:intergenic 30.0%
!! TTATGGTCTAGAATTTGTTG+TGG + chr4.4:32672447-32672466 None:intergenic 30.0%
!!! ATTGTTTCCTTTTAGGGTTA+TGG - chr4.4:32672529-32672548 MS.gene006564:intron 30.0%
!!! ATTTTTTTTTTCCTTCTGGC+AGG - chr4.4:32673900-32673919 MS.gene006564:CDS 30.0%
!!! TTGAGTGATCATGTTGTTTT+GGG + chr4.4:32670957-32670976 None:intergenic 30.0%
AAACAGTCTCGCAAAACTTA+TGG - chr4.4:32672379-32672398 MS.gene006564:CDS 35.0%
AAGAAACTAGAGCTATTAGG+TGG - chr4.4:32671394-32671413 MS.gene006564:intron 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr4.4:32671307-32671326 None:intergenic 35.0%
ACCCAGATGAAGAATCAATA+AGG + chr4.4:32671120-32671139 None:intergenic 35.0%
AGCAAATATAGTAAAGGGGA+GGG + chr4.4:32672869-32672888 None:intergenic 35.0%
AGTCATTCCATAACCCTAAA+AGG + chr4.4:32672539-32672558 None:intergenic 35.0%
ATGATATTTCTCAAGGACAC+AGG + chr4.4:32672919-32672938 None:intergenic 35.0%
ATTAAACAGGAAGATACTGC+TGG - chr4.4:32671529-32671548 MS.gene006564:intron 35.0%
ATTTCACCAACACTACTAAG+AGG + chr4.4:32671316-32671335 None:intergenic 35.0%
CAGAAAATGAGAAGGATTTG+AGG - chr4.4:32671440-32671459 MS.gene006564:CDS 35.0%
CAGCAAGACAAGAAAAAAGA+AGG + chr4.4:32673162-32673181 None:intergenic 35.0%
CCCAGATGAAGAATCAATAA+GGG + chr4.4:32671119-32671138 None:intergenic 35.0%
CTAATAACTCCTATGCTCAA+AGG + chr4.4:32671937-32671956 None:intergenic 35.0%
CTCAGGATACTTGAATAATG+AGG - chr4.4:32673227-32673246 MS.gene006564:CDS 35.0%
GCTGGTATATTGTATTCATC+TGG - chr4.4:32671547-32671566 MS.gene006564:intron 35.0%
GCTTCTGCTTTACACAAAAT+GGG - chr4.4:32671184-32671203 MS.gene006564:CDS 35.0%
GTCAAGAAACTAGAGCTATT+AGG - chr4.4:32671391-32671410 MS.gene006564:intron 35.0%
GTTACCTTTAGTCAAATCCA+TGG - chr4.4:32671162-32671181 MS.gene006564:intron 35.0%
TTCCCCAATTTCTTCATCTT+CGG + chr4.4:32673695-32673714 None:intergenic 35.0%
TTGAATCCTCTTAGTAGTGT+TGG - chr4.4:32671307-32671326 MS.gene006564:intron 35.0%
TTGAGCATAGGAGTTATTAG+TGG - chr4.4:32671937-32671956 MS.gene006564:intron 35.0%
TTGCCAATGTTTCATGTGTA+TGG - chr4.4:32671697-32671716 MS.gene006564:CDS 35.0%
! AGAAAAAAGAAGGTACAGGT+AGG + chr4.4:32673152-32673171 None:intergenic 35.0%
! ATAAGTTTTGCGAGACTGTT+TGG + chr4.4:32672380-32672399 None:intergenic 35.0%
! CTCAAATCCTTCTCATTTTC+TGG + chr4.4:32671442-32671461 None:intergenic 35.0%
! GACAAGAAAAAAGAAGGTAC+AGG + chr4.4:32673156-32673175 None:intergenic 35.0%
! TAAGTTTTGCGAGACTGTTT+GGG + chr4.4:32672379-32672398 None:intergenic 35.0%
! TGTTGGTGAAATTCGTAAAC+AGG - chr4.4:32671324-32671343 MS.gene006564:intron 35.0%
! TTTTCCCACCTAGTGAAAAA+AGG + chr4.4:32673058-32673077 None:intergenic 35.0%
!! AATCTTCTAAAGTGCCTCTT+TGG - chr4.4:32673622-32673641 MS.gene006564:CDS 35.0%
!! AGAGGCACTTTAGAAGATTA+CGG + chr4.4:32673621-32673640 None:intergenic 35.0%
!! AGTGAAAAAAGGCACTGATA+AGG + chr4.4:32673047-32673066 None:intergenic 35.0%
!! ATTTTCTGGCACAGCAATAA+TGG + chr4.4:32671428-32671447 None:intergenic 35.0%
!! CCCTTATTGATTCTTCATCT+GGG - chr4.4:32671116-32671135 MS.gene006564:intron 35.0%
!! GTCGTTTTCATAAGCTATCT+TGG - chr4.4:32672289-32672308 MS.gene006564:CDS 35.0%
!! GTTGAGTGATCATGTTGTTT+TGG + chr4.4:32670958-32670977 None:intergenic 35.0%
!! TTTTCTGGCACAGCAATAAT+GGG + chr4.4:32671427-32671446 None:intergenic 35.0%
!!! ATTTTTTCACACCCTCATGA+TGG - chr4.4:32671085-32671104 MS.gene006564:intron 35.0%
!!! CCCTAACGTTGATTTTATAC+AGG - chr4.4:32671975-32671994 MS.gene006564:intron 35.0%
!!! TATCAGTGCCTTTTTTCACT+AGG - chr4.4:32673047-32673066 MS.gene006564:CDS 35.0%
!!! TTTTTTCACACCCTCATGAT+GGG - chr4.4:32671086-32671105 MS.gene006564:intron 35.0%
AAAGGTCTTGATTCAATGGC+TGG - chr4.4:32672790-32672809 MS.gene006564:intron 40.0%
AAAGTGGTAGACTGGAACAA+TGG - chr4.4:32672649-32672668 MS.gene006564:intron 40.0%
AACAGCTAGAAACACACTCT+TGG + chr4.4:32671677-32671696 None:intergenic 40.0%
AATCAACGTTAGGGAAAGCA+TGG + chr4.4:32671969-32671988 None:intergenic 40.0%
ACATAATCGACCACATGCTT+CGG + chr4.4:32673763-32673782 None:intergenic 40.0%
ACTGTTATATAGGGCCTGTT+TGG - chr4.4:32672156-32672175 MS.gene006564:intron 40.0%
AGAAATTGGGGAAATTCCAG+TGG - chr4.4:32673702-32673721 MS.gene006564:CDS 40.0%
ATGAATACCAATGAGGGTGA+GGG + chr4.4:32670988-32671007 None:intergenic 40.0%
ATGTCAAGAAAAGGGTCAGT+TGG + chr4.4:32671055-32671074 None:intergenic 40.0%
ATTATTGTCGTTGGGCTCTA+CGG - chr4.4:32671741-32671760 MS.gene006564:intron 40.0%
ATTGTCAATGCCGACAACAT+CGG + chr4.4:32671850-32671869 None:intergenic 40.0%
CACAAAATGGGTGTTTCACA+AGG - chr4.4:32671196-32671215 MS.gene006564:CDS 40.0%
CTATTCGTCAATAGGGTTGT+TGG - chr4.4:32672609-32672628 MS.gene006564:intron 40.0%
CTCTTGGCTGTTCTGTATTT+AGG - chr4.4:32671268-32671287 MS.gene006564:intron 40.0%
GAAGCCATGGATTTGACTAA+AGG + chr4.4:32671169-32671188 None:intergenic 40.0%
GAGCAAATATAGTAAAGGGG+AGG + chr4.4:32672870-32672889 None:intergenic 40.0%
GAGGAAATTTGACATTGACG+AGG - chr4.4:32671774-32671793 MS.gene006564:intron 40.0%
GGCTTCTGCTTTACACAAAA+TGG - chr4.4:32671183-32671202 MS.gene006564:CDS 40.0%
GTGCCGAAGATGAAGAAATT+GGG - chr4.4:32673689-32673708 MS.gene006564:CDS 40.0%
GTTGTGGATGTGAACATCAA+TGG + chr4.4:32672431-32672450 None:intergenic 40.0%
TACAGGTAGGCTAAGGAATA+AGG + chr4.4:32673139-32673158 None:intergenic 40.0%
TGACATTGACGAGGTTATCA+GGG - chr4.4:32671783-32671802 MS.gene006564:intron 40.0%
TGCCGAAGATGAAGAAATTG+GGG - chr4.4:32673690-32673709 MS.gene006564:CDS 40.0%
TTCTTGACAACAAATGCCAC+TGG + chr4.4:32673721-32673740 None:intergenic 40.0%
TTGACATTGACGAGGTTATC+AGG - chr4.4:32671782-32671801 MS.gene006564:intron 40.0%
TTTGTGTAAAGCAGAAGCCA+TGG + chr4.4:32671182-32671201 None:intergenic 40.0%
! AAAGATGGTTGGCTACATAC+AGG - chr4.4:32673273-32673292 MS.gene006564:CDS 40.0%
! AGAACAGCCAAGAGTATAAC+AGG + chr4.4:32671262-32671281 None:intergenic 40.0%
! AGTGCCGAAGATGAAGAAAT+TGG - chr4.4:32673688-32673707 MS.gene006564:CDS 40.0%
! GAACAGCCAAGAGTATAACA+GGG + chr4.4:32671261-32671280 None:intergenic 40.0%
! GAGCAGGTTCTTCTTTTGAT+TGG - chr4.4:32673787-32673806 MS.gene006564:CDS 40.0%
! GCACTTTAGAAGATTACGGA+TGG + chr4.4:32673617-32673636 None:intergenic 40.0%
! GCATTGACAATGAAGGCAAA+GGG - chr4.4:32671859-32671878 MS.gene006564:intron 40.0%
! TAAGTTGCAGAGTTTGAGAC+AGG - chr4.4:32671894-32671913 MS.gene006564:intron 40.0%
! TTGTATTCATCTGGGACTAC+AGG - chr4.4:32671556-32671575 MS.gene006564:intron 40.0%
! TTTTTCACACCCTCATGATG+GGG - chr4.4:32671087-32671106 MS.gene006564:intron 40.0%
!! AGTGGCATTTGTTGTCAAGA+AGG - chr4.4:32673720-32673739 MS.gene006564:CDS 40.0%
!! ATGAAGGCAAAGGGTGTTAA+TGG - chr4.4:32671868-32671887 MS.gene006564:intron 40.0%
!! GCCCTTATTGATTCTTCATC+TGG - chr4.4:32671115-32671134 MS.gene006564:intron 40.0%
!!! AATGGTACTTTTTTACCCCC+AGG - chr4.4:32672667-32672686 MS.gene006564:intron 40.0%
!!! AGTGCCTTTTTTCACTAGGT+GGG - chr4.4:32673051-32673070 MS.gene006564:CDS 40.0%
AACACAGCTTCTAGATCAGC+AGG + chr4.4:32673498-32673517 None:intergenic 45.0%
AGAGGGCCTTCTATTATGAG+AGG - chr4.4:32672712-32672731 MS.gene006564:intron 45.0%
CAAGAAAAGGGTCAGTTGGA+AGG + chr4.4:32671051-32671070 None:intergenic 45.0%
CGTAAACAGGTTCAAGAGTG+TGG - chr4.4:32671337-32671356 MS.gene006564:intron 45.0%
GATGAATACCAATGAGGGTG+AGG + chr4.4:32670989-32671008 None:intergenic 45.0%
GCTCTACGGTCATTGTAATG+AGG - chr4.4:32671755-32671774 MS.gene006564:intron 45.0%
GGGAGGGAGTCTTGATATAA+AGG + chr4.4:32672853-32672872 None:intergenic 45.0%
TCTTCACCGACAAGATACCA+AGG - chr4.4:32673953-32673972 MS.gene006564:CDS 45.0%
TGCTGTGCCAGAAAATGAGA+AGG - chr4.4:32671432-32671451 MS.gene006564:CDS 45.0%
TGGTTTATCGCTCTTTGCTG+CGG - chr4.4:32671717-32671736 MS.gene006564:intron 45.0%
TGTACTTTCTCCGAAGCATG+TGG - chr4.4:32673750-32673769 MS.gene006564:CDS 45.0%
TGTTGTTCCACCGATGTTGT+CGG - chr4.4:32671837-32671856 MS.gene006564:intron 45.0%
TTGCTCCATACAAGAAGGTC+CGG - chr4.4:32673923-32673942 MS.gene006564:CDS 45.0%
! AAGAAGGTACAGGTAGGCTA+AGG + chr4.4:32673146-32673165 None:intergenic 45.0%
! AGTTTGAGACAGGTCTCTTG+CGG - chr4.4:32671904-32671923 MS.gene006564:intron 45.0%
!! AAAGGTTTGAGTTGCTGCAG+CGG + chr4.4:32672835-32672854 None:intergenic 45.0%
!! CTTCATCTTCGGCACTGATA+TGG + chr4.4:32673684-32673703 None:intergenic 45.0%
!! GCATTTGTTGTCAAGAAGGC+TGG - chr4.4:32673724-32673743 MS.gene006564:CDS 45.0%
!! GGCATTGACAATGAAGGCAA+AGG - chr4.4:32671858-32671877 MS.gene006564:intron 45.0%
!! GTTGTCGGCATTGACAATGA+AGG - chr4.4:32671852-32671871 MS.gene006564:intron 45.0%
!!! CAGTGCCTTTTTTCACTAGG+TGG - chr4.4:32673050-32673069 MS.gene006564:CDS 45.0%
AAGATACCAAGGTCTCCGAC+TGG - chr4.4:32673964-32673983 MS.gene006564:CDS 50.0%
AATGACTGAGTCGACTGCAG+TGG - chr4.4:32672552-32672571 MS.gene006564:intron 50.0%
ACCTTCCGGACCTTCTTGTA+TGG + chr4.4:32673931-32673950 None:intergenic 50.0%
AGATACCAAGGTCTCCGACT+GGG - chr4.4:32673965-32673984 MS.gene006564:CDS 50.0%
AGATCCTCCGAAAGCAACTC+AGG - chr4.4:32673989-32674008 MS.gene006564:CDS 50.0%
AGGGCTAAAACCCCATCATG+AGG + chr4.4:32671100-32671119 None:intergenic 50.0%
ATGACTGAGTCGACTGCAGT+GGG - chr4.4:32672553-32672572 MS.gene006564:intron 50.0%
CGGAGACCTTGGTATCTTGT+CGG + chr4.4:32673962-32673981 None:intergenic 50.0%
GACAACATCGGTGGAACAAC+AGG + chr4.4:32671838-32671857 None:intergenic 50.0%
GCAGGTTGCTCCATACAAGA+AGG - chr4.4:32673918-32673937 MS.gene006564:CDS 50.0%
GCTGCGGGATTATTGTCGTT+GGG - chr4.4:32671733-32671752 MS.gene006564:intron 50.0%
GGGACTACAGGTGTGAGTAA+AGG - chr4.4:32671568-32671587 MS.gene006564:intron 50.0%
GGGCTAAAACCCCATCATGA+GGG + chr4.4:32671099-32671118 None:intergenic 50.0%
GGGTCAGTTGGAAGGTTTCT+AGG + chr4.4:32671043-32671062 None:intergenic 50.0%
GGTCGATTATGTTGCTGAGC+AGG - chr4.4:32673771-32673790 MS.gene006564:CDS 50.0%
GGTTTATCGCTCTTTGCTGC+GGG - chr4.4:32671718-32671737 MS.gene006564:intron 50.0%
TCCAAACACGGAGGCAAAAG+TGG - chr4.4:32672633-32672652 MS.gene006564:intron 50.0%
TCCATACAAGAAGGTCCGGA+AGG - chr4.4:32673927-32673946 MS.gene006564:CDS 50.0%
TGCTGCGGGATTATTGTCGT+TGG - chr4.4:32671732-32671751 MS.gene006564:intron 50.0%
TGTCGGTGAAGAACACCTTC+CGG + chr4.4:32673945-32673964 None:intergenic 50.0%
TTTCGGAGGATCTTCCCAGT+CGG + chr4.4:32673982-32674001 None:intergenic 50.0%
! AGCAGTGCCTGAGTTGCTTT+CGG + chr4.4:32673999-32674018 None:intergenic 50.0%
!! ACCACTTTTGCCTCCGTGTT+TGG + chr4.4:32672637-32672656 None:intergenic 50.0%
!! ACGGCTGCATCGACTATTTC+AGG + chr4.4:32673531-32673550 None:intergenic 50.0%
!! AGGGTTGTTGGCTCCAAACA+CGG - chr4.4:32672621-32672640 MS.gene006564:intron 50.0%
ACCAATGAGGGTGAGGGTGA+GGG + chr4.4:32670982-32671001 None:intergenic 55.0%
ATCTTCCCAGTCGGAGACCT+TGG + chr4.4:32673973-32673992 None:intergenic 55.0%
CAGCGGTGTGACTAACGTTC+AGG + chr4.4:32672818-32672837 None:intergenic 55.0%
CGGAGGCAAAAGTGGTAGAC+TGG - chr4.4:32672641-32672660 MS.gene006564:intron 55.0%
GGTGCAGCACCTTTGAGCAT+AGG - chr4.4:32671925-32671944 MS.gene006564:intron 55.0%
GTATGGAGCAACCTGCCAGA+AGG + chr4.4:32673914-32673933 None:intergenic 55.0%
GTCAATGCCGACAACATCGG+TGG + chr4.4:32671847-32671866 None:intergenic 55.0%
TACCAATGAGGGTGAGGGTG+AGG + chr4.4:32670983-32671002 None:intergenic 55.0%
! AGTGCCTGAGTTGCTTTCGG+AGG + chr4.4:32673996-32674015 None:intergenic 55.0%
! GCGGTGAGCTTTGGTTGAGA+GGG - chr4.4:32672695-32672714 MS.gene006564:intron 55.0%
! TGCGGTGAGCTTTGGTTGAG+AGG - chr4.4:32672694-32672713 MS.gene006564:intron 55.0%
! TTTTTACCCCCAGGCAGCTG+CGG - chr4.4:32672676-32672695 MS.gene006564:intron 55.0%
!! GTTGTTGGCTCCAAACACGG+AGG - chr4.4:32672624-32672643 MS.gene006564:intron 55.0%
AAAGCTCACCGCAGCTGCCT+GGG + chr4.4:32672687-32672706 None:intergenic 60.0%
ACCCTCACCCTCACCCTCAT+TGG - chr4.4:32670978-32670997 MS.gene006564:CDS 60.0%
TCGACTGCAGTGGGAACACG+TGG - chr4.4:32672562-32672581 MS.gene006564:intron 60.0%
AAGCTCACCGCAGCTGCCTG+GGG + chr4.4:32672686-32672705 None:intergenic 65.0%
CAAAGCTCACCGCAGCTGCC+TGG + chr4.4:32672688-32672707 None:intergenic 65.0%
CAGGCAGCTGCGGTGAGCTT+TGG - chr4.4:32672686-32672705 MS.gene006564:intron 65.0%
AGCTCACCGCAGCTGCCTGG+GGG + chr4.4:32672685-32672704 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 32670920 32674035 32670920 ID=MS.gene006564
chr4.4 mRNA 32670920 32674035 32670920 ID=MS.gene006564.t1;Parent=MS.gene006564
chr4.4 exon 32672959 32674035 32672959 ID=MS.gene006564.t1.exon1;Parent=MS.gene006564.t1
chr4.4 CDS 32672959 32674035 32672959 ID=cds.MS.gene006564.t1;Parent=MS.gene006564.t1
chr4.4 exon 32672222 32672411 32672222 ID=MS.gene006564.t1.exon2;Parent=MS.gene006564.t1
chr4.4 CDS 32672222 32672411 32672222 ID=cds.MS.gene006564.t1;Parent=MS.gene006564.t1
chr4.4 exon 32671578 32671723 32671578 ID=MS.gene006564.t1.exon3;Parent=MS.gene006564.t1
chr4.4 CDS 32671578 32671723 32671578 ID=cds.MS.gene006564.t1;Parent=MS.gene006564.t1
chr4.4 exon 32671399 32671466 32671399 ID=MS.gene006564.t1.exon4;Parent=MS.gene006564.t1
chr4.4 CDS 32671399 32671466 32671399 ID=cds.MS.gene006564.t1;Parent=MS.gene006564.t1
chr4.4 exon 32671163 32671265 32671163 ID=MS.gene006564.t1.exon5;Parent=MS.gene006564.t1
chr4.4 CDS 32671163 32671265 32671163 ID=cds.MS.gene006564.t1;Parent=MS.gene006564.t1
chr4.4 exon 32670920 32671033 32670920 ID=MS.gene006564.t1.exon6;Parent=MS.gene006564.t1
chr4.4 CDS 32670920 32671033 32670920 ID=cds.MS.gene006564.t1;Parent=MS.gene006564.t1
Gene Sequence

>MS.gene006564

ATGGCTTCTGCTGCAAATTTAAACTGTGAAATCACCCAAAACAACATGATCACTCAACACCCTCACCCTCACCCTCATTGGTATTCATCAAAAACTGGAATTTACAACAGTGTACACTCTCCTAGAAACCTTCCAACTGACCCTTTTCTTGACATTGTTTCTTTCATTTTTTCACACCCTCATGATGGGGTTTTAGCCCTTATTGATTCTTCATCTGGGTCTTCCATTTCTTACTCAAAGTTGTTACCTTTAGTCAAATCCATGGCTTCTGCTTTACACAAAATGGGTGTTTCACAAGGTGATGTTGTTTTGCTTCTACTTCCAAATTCAATTTATTACCCTGTTATACTCTTGGCTGTTCTGTATTTAGGTGCTGTTTTTACACCATTGAATCCTCTTAGTAGTGTTGGTGAAATTCGTAAACAGGTTCAAGAGTGTGGTGTGAGTTTTGCTTTTACTATACCTGAAAATGTCAAGAAACTAGAGCTATTAGGTGGCATTAATCCCATTATTGCTGTGCCAGAAAATGAGAAGGATTTGAGGAATGAGTGTTTCTCAAGTTTTTTTAACCTTCTTTATGGTAAATTTGATTTGCCTCAAAGGGTAGTTATTAAACAGGAAGATACTGCTGGTATATTGTATTCATCTGGGACTACAGGTGTGAGTAAAGGAGTTGTTTTAACTCATAGAAATCTTATTTCTATGGTTGAGCTTTTTGTGAGATTTGAAGCTTCTCAATATGATTACTCTTGTTCCAAGAGTGTGTTTCTAGCTGTTTTGCCAATGTTTCATGTGTATGGTTTATCGCTCTTTGCTGCGGGATTATTGTCGTTGGGCTCTACGGTCATTGTAATGAGGAAATTTGACATTGACGAGGTTATCAGGGTGATTGACAAATATAATGTTACACACTTTCCTGTTGTTCCACCGATGTTGTCGGCATTGACAATGAAGGCAAAGGGTGTTAATGGAAGTAAGTTGCAGAGTTTGAGACAGGTCTCTTGCGGTGCAGCACCTTTGAGCATAGGAGTTATTAGTGGTTTTGTCCATGCTTTCCCTAACGTTGATTTTATACAGGTAAAGAACCTTTGAATAGAATAGTCTTAATTAGAGAATGCAAGTAATGCTCCATCGTTTATATGATTGCTTGAGTTAATGTAGTTATTTCATTTAACTAAATGCTGATTATTTCATTTAATTATGAGAGAACGTGTGAGCTTTAAAAATTTAATAGCAACTGTTATATAGGGCCTGTTTGGCTAAACAATTTATTTGCTGCTTATAGCATAAGCAGTTTTCATACACTTACATAAGCTATTTCTATAGCAAAAAATAAAATAAAGTAAAATTGTTTTTGTATATGTTAAAAGTCGTTTTCATAAGCTATCTTGGACAACTTATAAAAATAAGCTGAAAAGAACTAAGGAAAAATGTCAAAGCTGTTCTCATAAGTTCTCCCAAACAGTCTCGCAAAACTTATGGCAGCAGATAAGCTCAAATAAGTTGTGCCATTGATGTTCACATCCACAACAAATTCTAGACCATAAAAGTTGAACTTTATTTGTTTATGATAATAATGTTGTTTTACTAGATTATTAATAGCTGTAAGATTGTTTCCTTTTAGGGTTATGGAATGACTGAGTCGACTGCAGTGGGAACACGTGGCTTCAATACTGAAAAGTTTCACAACTATTCGTCAATAGGGTTGTTGGCTCCAAACACGGAGGCAAAAGTGGTAGACTGGAACAATGGTACTTTTTTACCCCCAGGCAGCTGCGGTGAGCTTTGGTTGAGAGGGCCTTCTATTATGAGAGGTAAGAAATCTGCATTTTTCTTAATAGATATTAAAAGTGAAACACGGTAGTAATATAAAGGTCTTGATTCAATGGCTGGAACCTGAACGTTAGTCACACCGCTGCAGCAACTCAAACCTTTATATCAAGACTCCCTCCCCTTTACTATATTTGCTCTATATTTGTTAACGCTTAACTAAAGGCCTGTGTCCTTGAGAAATATCATGTTAGTTTAGTAGACATTTTATTCTTCTAGAGTGATGCTATACACTCATCTGTTGCTGTAGTGCAACTATTGACGCTAATTCAGTTGAAAGATACATATACATCTCCTTATCAGTGCCTTTTTTCACTAGGTGGGAAAACTGAAGTAAATTTCTCTTGGAGTCAACTGTTTCATTGCTATAATAACTTTTGTTTCTGCCTTATTCCTTAGCCTACCTGTACCTTCTTTTTTCTTGTCTTGCTGCTATTCGTCCAATTAAAATTGTTAATCAAACTCTTTCCTTTAATTCTCAGGATACTTGAATAATGAGGAAGCTACTATATCAACAATTGATAAAGATGGTTGGCTACATACAGGAGATATTGTTTATTTTGATCAGGATGGATATTTACATTTGGCTGACCGCTTGAAAGACATTATCAAATACAAGGGATTTCAGGTATCTACTTTTAGGTTTATTAAATTTTATAAAGAAAAGCGAGGATAGATCAACGAGAAATGCAGAATTTTACTGAAATGCACACTTTGTATGACACATGATCTGTTACAGATTGCTCCTGCTGATCTAGAAGCTGTGTTAATTTTGCATCCTGAAATAGTCGATGCAGCCGTTACAGCGTAAGTATATGAACACTTCATTTATGTCTTCGCTTCTGCAATCTATAGAAAATGCAGCCATCCGTAATCTTCTAAAGTGCCTCTTTGGACATTTGATTATAACCTCAAATGTTACGTCATTTTTCCATATCAGTGCCGAAGATGAAGAAATTGGGGAAATTCCAGTGGCATTTGTTGTCAAGAAGGCTGGAAGTGTACTTTCTCCGAAGCATGTGGTCGATTATGTTGCTGAGCAGGTTCTTCTTTTGATTGGACAGACATGATTTTTATGATATTTGAATTGATATTACTAATTGATATTACTACTTGAATCAATGTCATGCTTTTCACATTTTAACCCTTTATTTTTTTTTTCCTTCTGGCAGGTTGCTCCATACAAGAAGGTCCGGAAGGTGTTCTTCACCGACAAGATACCAAGGTCTCCGACTGGGAAGATCCTCCGAAAGCAACTCAGGCACTGCTTGAGTTCTAAACTCTAA
Protein sequence

>MS.gene006564.t1

MASAANLNCEITQNNMITQHPHPHPHWYSSKTGIYNSVHSPRNLPTDPFLDIVSFIFSHPHDGVLALIDSSSGSSISYSKLLPLVKSMASALHKMGVSQGDVVLLLLPNSIYYPVILLAVLYLGAVFTPLNPLSSVGEIRKQVQECGVSFAFTIPENVKKLELLGGINPIIAVPENEKDLRNECFSSFFNLLYGKFDLPQRVVIKQEDTAGILYSSGTTGVSKGVVLTHRNLISMVELFVRFEASQYDYSCSKSVFLAVLPMFHVYGLSLFAAGLLSLGSTVIVMRKFDIDEVIRVIDKYNVTHFPVVPPMLSALTMKAKGVNGSKLQSLRQVSCGAAPLSIGVISGFVHAFPNVDFIQGYGMTESTAVGTRGFNTEKFHNYSSIGLLAPNTEAKVVDWNNGTFLPPGSCGELWLRGPSIMRGYLNNEEATISTIDKDGWLHTGDIVYFDQDGYLHLADRLKDIIKYKGFQIAPADLEAVLILHPEIVDAAVTAAEDEEIGEIPVAFVVKKAGSVLSPKHVVDYVAEQVAPYKKVRKVFFTDKIPRSPTGKILRKQLRHCLSSKL