AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04301

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04301.t1 MTR_3g460770 97.653 554 12 1 1 554 1 553 0.0 1106
MS.gene04301.t1 MTR_2g105570 39.154 544 311 9 10 538 7 545 1.17e-137 410
MS.gene04301.t1 MTR_4g005750 39.552 536 312 7 11 538 7 538 2.85e-130 391
MS.gene04301.t1 MTR_5g007640 37.893 541 315 8 11 538 51 583 4.35e-127 384
MS.gene04301.t1 MTR_4g128337 38.519 540 318 6 10 540 12 546 7.80e-122 370
MS.gene04301.t1 MTR_4g057730 35.424 542 332 8 13 545 9 541 4.04e-120 365
MS.gene04301.t1 MTR_2g095510 36.245 538 330 7 12 542 8 539 1.08e-116 356
MS.gene04301.t1 MTR_1g026310 34.791 549 316 9 8 532 20 550 2.12e-114 351
MS.gene04301.t1 MTR_1g090737 38.247 502 298 5 36 532 62 556 1.04e-113 349
MS.gene04301.t1 MTR_1g063120 35.424 542 320 11 23 542 22 555 1.52e-104 325
MS.gene04301.t1 MTR_8g039720 34.862 545 323 12 8 532 17 549 3.01e-102 319
MS.gene04301.t1 MTR_4g077900 37.190 484 286 9 70 542 2 478 4.37e-99 308
MS.gene04301.t1 MTR_5g027480 34.823 537 334 8 15 542 26 555 3.00e-98 309
MS.gene04301.t1 MTR_1g063110 33.648 529 336 8 16 532 13 538 4.48e-98 308
MS.gene04301.t1 MTR_4g057730 34.615 468 292 7 13 471 9 471 1.20e-97 305
MS.gene04301.t1 MTR_3g088870 35.000 500 299 8 44 532 64 548 2.40e-95 301
MS.gene04301.t1 MTR_3g088880 35.354 495 301 8 44 532 65 546 4.84e-95 301
MS.gene04301.t1 MTR_5g027500 34.128 545 339 8 15 542 27 568 1.33e-89 287
MS.gene04301.t1 MTR_1g073180 35.630 508 312 9 34 539 37 531 4.87e-87 279
MS.gene04301.t1 MTR_4g057730 33.184 446 284 7 13 449 9 449 1.49e-86 275
MS.gene04301.t1 MTR_2g095510 33.708 445 282 7 12 449 8 446 4.08e-82 264
MS.gene04301.t1 MTR_1g073180 35.135 481 297 9 34 512 59 526 2.13e-78 256
MS.gene04301.t1 MTR_3g035130 27.912 498 331 12 57 541 32 514 4.05e-54 191
MS.gene04301.t1 MTR_1569s0010 27.043 514 333 10 57 535 22 528 5.84e-53 189
MS.gene04301.t1 MTR_1g026430 28.500 400 244 9 8 383 20 401 2.05e-52 184
MS.gene04301.t1 MTR_1g026470 28.607 402 245 9 8 385 20 403 2.59e-52 184
MS.gene04301.t1 MTR_0803s0020 26.369 493 319 13 59 528 263 734 2.79e-43 166
MS.gene04301.t1 MTR_3g035130 27.982 436 286 12 57 479 32 452 4.80e-42 157
MS.gene04301.t1 MTR_5g008800 26.128 532 350 15 34 537 19 535 9.11e-41 155
MS.gene04301.t1 MTR_5g076240 28.185 518 317 17 58 536 41 542 1.71e-40 154
MS.gene04301.t1 MTR_3g064420 25.668 561 330 16 34 537 19 549 1.53e-39 152
MS.gene04301.t1 MTR_8g074040 23.596 534 363 13 34 537 61 579 3.67e-39 151
MS.gene04301.t1 MTR_2g044180 26.404 534 319 15 58 540 47 557 7.31e-39 150
MS.gene04301.t1 MTR_5g008820 24.953 537 348 15 34 537 19 533 8.06e-38 147
MS.gene04301.t1 MTR_4g121600 26.616 526 328 16 58 540 47 557 7.11e-36 141
MS.gene04301.t1 MTR_8g074120 28.101 395 250 14 160 537 113 490 3.01e-35 139
MS.gene04301.t1 MTR_8g074040 25.231 432 291 11 127 537 41 461 1.04e-34 137
MS.gene04301.t1 MTR_2g044180 28.074 431 248 13 149 540 59 466 8.80e-33 131
MS.gene04301.t1 MTR_5g023730 25.140 537 353 16 34 538 21 540 5.09e-32 130
MS.gene04301.t1 MTR_5g076220 25.184 544 347 17 34 536 19 543 1.17e-30 126
MS.gene04301.t1 MTR_5g076200 24.453 548 346 19 34 536 19 543 3.98e-28 119
MS.gene04301.t1 MTR_0161s0050 23.519 574 361 20 14 536 3 549 4.27e-26 112
MS.gene04301.t1 MTR_4g011790 25.946 370 240 12 192 536 233 593 2.19e-24 107
MS.gene04301.t1 MTR_4g011790 26.171 363 234 12 192 529 233 586 1.50e-23 105
MS.gene04301.t1 MTR_4g013210 24.933 373 244 11 192 536 76 440 1.87e-23 103
MS.gene04301.t1 MTR_2g030240 23.897 544 340 17 58 547 29 552 3.59e-22 100
MS.gene04301.t1 MTR_4g011790 25.594 379 239 13 192 536 233 602 3.75e-22 100
MS.gene04301.t1 MTR_4g013210 25.926 324 214 10 192 497 228 543 4.54e-21 97.4
MS.gene04301.t1 MTR_2g030240 24.685 397 241 12 192 547 79 458 2.18e-20 94.7
MS.gene04301.t1 MTR_4g013210 24.242 363 218 10 192 536 76 399 2.32e-20 94.0
MS.gene04301.t1 MTR_1054s0020 23.392 513 350 17 42 537 281 767 4.09e-20 94.7
MS.gene04301.t1 MTR_4g057755 46.667 75 40 0 398 472 46 120 4.65e-20 86.7
MS.gene04301.t1 MTR_4g013210 24.054 370 224 10 185 536 221 551 5.76e-20 94.0
MS.gene04301.t1 MTR_7g084850 22.261 566 371 18 43 552 185 737 6.84e-20 94.0
MS.gene04301.t1 MTR_7g109830 26.285 506 265 23 48 469 66 547 8.75e-19 90.5
MS.gene04301.t1 MTR_1g104810 32.620 187 106 8 286 459 389 568 1.02e-18 90.5
MS.gene04301.t1 MTR_1g104810 33.333 180 100 8 293 459 329 501 1.06e-18 90.1
MS.gene04301.t1 MTR_5g009360 26.389 360 202 16 162 469 75 423 3.05e-18 88.6
MS.gene04301.t1 MTR_1g026350 28.571 126 88 1 15 140 26 149 4.23e-18 82.4
MS.gene04301.t1 MTR_5g009360 26.389 360 202 16 162 469 201 549 4.46e-18 88.2
MS.gene04301.t1 MTR_8g083170 36.735 196 105 7 330 512 475 664 1.47e-17 86.7
MS.gene04301.t1 MTR_3g084470 36.571 175 97 5 330 493 468 639 2.15e-17 86.3
MS.gene04301.t1 MTR_5g009350 26.536 358 204 14 162 469 201 549 4.16e-17 85.1
MS.gene04301.t1 MTR_5g009350 26.257 358 205 14 162 469 201 549 4.56e-17 84.7
MS.gene04301.t1 MTR_5g009350 26.536 358 204 14 162 469 201 549 4.64e-17 85.1
MS.gene04301.t1 MTR_5g009350 26.462 359 205 14 162 470 201 550 4.80e-17 84.7
MS.gene04301.t1 MTR_8g099420 25.770 357 200 14 162 465 202 546 8.43e-17 84.3
MS.gene04301.t1 MTR_3g084450 33.168 202 113 7 330 518 264 456 3.88e-16 81.6
MS.gene04301.t1 MTR_3g084450 33.168 202 113 7 330 518 472 664 5.48e-16 81.6
MS.gene04301.t1 MTR_3g088890 48.333 60 31 0 444 503 10 69 2.89e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04301.t1 AT1G62940 72.932 532 141 2 10 539 7 537 0.0 789
MS.gene04301.t1 AT3G21240 38.060 536 319 6 12 538 19 550 4.45e-130 391
MS.gene04301.t1 AT1G65060 38.534 532 316 6 14 538 33 560 1.04e-126 383
MS.gene04301.t1 AT4G05160 36.716 542 325 8 13 542 9 544 1.51e-123 374
MS.gene04301.t1 AT1G20480 38.278 546 314 7 11 542 29 565 1.68e-123 374
MS.gene04301.t1 AT1G20510 35.755 523 325 7 16 532 17 534 2.59e-122 371
MS.gene04301.t1 AT3G21230 37.071 553 323 8 6 538 17 564 2.58e-118 361
MS.gene04301.t1 AT1G51680 37.291 539 318 8 15 538 24 557 2.30e-116 356
MS.gene04301.t1 AT3G21240 37.821 468 278 6 12 470 19 482 5.52e-108 332
MS.gene04301.t1 AT3G21240 37.821 468 278 6 12 470 19 482 5.52e-108 332
MS.gene04301.t1 AT5G63380 38.776 490 285 9 48 532 69 548 1.28e-106 331
MS.gene04301.t1 AT3G21230 36.484 529 305 9 6 514 17 534 1.63e-104 325
MS.gene04301.t1 AT1G51680 36.905 504 298 8 15 503 24 522 2.83e-103 322
MS.gene04301.t1 AT1G20500 32.083 533 346 8 8 532 16 540 1.07e-102 320
MS.gene04301.t1 AT4G19010 37.525 509 305 10 42 542 59 562 2.66e-102 320
MS.gene04301.t1 AT5G38120 33.087 541 334 9 16 542 24 550 9.93e-101 315
MS.gene04301.t1 AT1G20510 33.766 462 295 7 16 471 17 473 1.48e-98 307
MS.gene04301.t1 AT3G21230 36.495 485 283 8 6 470 17 496 6.03e-95 298
MS.gene04301.t1 AT1G51680 36.518 471 279 8 15 470 24 489 1.22e-93 295
MS.gene04301.t1 AT1G65060 35.360 444 276 6 15 451 34 473 4.14e-92 291
MS.gene04301.t1 AT5G38120 32.271 502 312 9 16 503 24 511 1.09e-87 280
MS.gene04301.t1 AT1G20510 32.045 440 288 7 16 449 17 451 4.58e-86 275
MS.gene04301.t1 AT5G38120 32.553 470 289 9 16 471 24 479 3.41e-80 259
MS.gene04301.t1 AT5G38120 32.553 470 289 9 16 471 24 479 3.41e-80 259
MS.gene04301.t1 AT3G21230 31.250 416 261 9 10 405 21 431 2.41e-53 187
MS.gene04301.t1 AT1G20490 27.869 427 278 10 8 418 16 428 2.27e-52 185
MS.gene04301.t1 AT1G20490 27.869 427 278 10 8 418 16 428 2.27e-52 185
MS.gene04301.t1 AT3G48990 28.599 521 342 12 35 541 9 513 5.58e-50 180
MS.gene04301.t1 AT1G21530 26.523 558 345 16 23 536 5 541 1.86e-41 157
MS.gene04301.t1 AT1G21530 26.523 558 347 16 23 536 5 543 5.86e-41 155
MS.gene04301.t1 AT5G16370 26.617 541 339 18 34 536 19 539 3.52e-40 154
MS.gene04301.t1 AT5G16340 27.140 549 339 19 34 541 19 547 1.15e-39 152
MS.gene04301.t1 AT1G21540 26.476 559 347 15 23 536 5 544 1.36e-39 152
MS.gene04301.t1 AT1G77240 25.698 537 359 16 29 536 14 539 4.20e-39 150
MS.gene04301.t1 AT1G75960 28.125 544 337 16 29 536 14 539 1.05e-38 149
MS.gene04301.t1 AT1G20560 27.491 542 334 17 34 537 21 541 3.16e-38 148
MS.gene04301.t1 AT2G17650 25.045 551 352 16 34 540 66 599 3.74e-37 145
MS.gene04301.t1 AT1G65890 26.335 543 349 15 34 536 19 550 2.17e-36 143
MS.gene04301.t1 AT1G66120 25.140 537 356 14 34 536 19 543 5.54e-36 142
MS.gene04301.t1 AT1G65880 26.103 544 350 15 34 536 19 551 5.92e-36 142
MS.gene04301.t1 AT1G20560 29.024 379 229 11 180 537 104 463 3.49e-32 129
MS.gene04301.t1 AT1G76290 27.222 540 341 19 34 542 19 537 5.61e-31 127
MS.gene04301.t1 AT3G16910 25.933 536 320 19 58 540 49 560 9.83e-31 126
MS.gene04301.t1 AT1G68270 24.490 539 324 16 34 531 19 515 1.03e-30 126
MS.gene04301.t1 AT5G27600 29.070 344 184 15 161 457 239 569 1.22e-25 112
MS.gene04301.t1 AT3G16170 21.730 497 350 10 70 538 119 604 7.27e-25 109
MS.gene04301.t1 AT3G05970 29.630 324 170 11 181 457 257 569 5.58e-23 103
MS.gene04301.t1 AT1G30520 22.563 554 350 13 47 543 23 554 2.29e-22 101
MS.gene04301.t1 AT1G30520 22.563 554 350 13 47 543 23 554 2.29e-22 101
MS.gene04301.t1 AT1G64400 25.813 492 283 20 49 469 70 550 7.86e-22 100
MS.gene04301.t1 AT4G23850 25.657 495 280 21 49 469 70 550 5.53e-20 94.4
MS.gene04301.t1 AT1G49430 23.984 492 286 21 38 455 60 537 2.54e-17 85.9
MS.gene04301.t1 AT1G30520 22.395 451 285 10 47 454 23 451 1.20e-14 77.0
MS.gene04301.t1 AT1G30520 22.395 451 285 10 47 454 23 451 1.20e-14 77.0
MS.gene04301.t1 AT3G23790 33.333 171 90 5 330 479 473 640 6.33e-14 75.1
MS.gene04301.t1 AT2G47240 34.320 169 102 7 305 469 325 488 1.58e-13 73.9
MS.gene04301.t1 AT4G14070 32.099 162 96 4 330 480 489 647 1.59e-13 73.9
MS.gene04301.t1 AT4G14070 26.556 241 149 7 27 241 95 333 2.19e-12 70.5
MS.gene04301.t1 AT2G47240 34.320 169 102 7 305 469 384 547 1.63e-13 73.9
MS.gene04301.t1 AT2G47240 34.320 169 102 7 305 469 384 547 1.63e-13 73.9
MS.gene04301.t1 AT2G47240 34.320 169 102 7 305 469 384 547 1.63e-13 73.9

Find 111 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAGGGATCTTCCTGTTTC+AGG 0.121636 3.4:-55953627 None:intergenic
CACCATATAGTTCAGCATTT+TGG 0.132681 3.4:-55952089 None:intergenic
GCTATTTGTGACAATTAATT+TGG 0.178651 3.4:-55952367 None:intergenic
ATGGCATACTCTACAATATT+TGG 0.200376 3.4:-55952246 None:intergenic
ATTGTTCCACCTATTATTCT+TGG 0.237238 3.4:+55952908 MS.gene04301:CDS
ATTGGAAAAGTCACTACATT+AGG 0.245143 3.4:+55952740 MS.gene04301:CDS
GCATTGCACAAAGGTCATTT+TGG 0.245241 3.4:-55952613 None:intergenic
TTAACCAATCCAAGAATAAT+AGG 0.258003 3.4:-55952917 None:intergenic
TGGTTTCATCCTTCCGAATT+TGG 0.259256 3.4:+55953587 MS.gene04301:CDS
GAAGATGGTGGAAAAGATTA+AGG 0.261374 3.4:+55954770 MS.gene04301:CDS
AATCTTTGTTCTACACTCTT+TGG 0.286307 3.4:+55952701 MS.gene04301:CDS
GAATATGTTGTGCAACTATT+AGG 0.306968 3.4:+55952798 MS.gene04301:CDS
CATGATTCCCAAAGCAATAA+TGG 0.317628 3.4:-55952265 None:intergenic
AGTTCCCCTGGTGTGTTCCT+TGG 0.340867 3.4:-55953648 None:intergenic
ATTTCCCCTGCCTCTTCATC+TGG 0.345841 3.4:-55954579 None:intergenic
TCAAAGGAAGTAAGTAATTC+AGG 0.346829 3.4:-55953016 None:intergenic
GAGTATGCCATTATTGCTTT+GGG 0.348885 3.4:+55952258 MS.gene04301:CDS
AGAGTATGCCATTATTGCTT+TGG 0.356223 3.4:+55952257 MS.gene04301:CDS
GGTCTCTTGGTTTGAGAAAA+GGG 0.362079 3.4:+55952202 MS.gene04301:CDS
GTTCCCCTGGTGTGTTCCTT+GGG 0.362580 3.4:-55953647 None:intergenic
AGGTCTCTTGGTTTGAGAAA+AGG 0.379071 3.4:+55952201 MS.gene04301:CDS
CTCCAAAATGCTGAACTATA+TGG 0.388619 3.4:+55952087 MS.gene04301:CDS
ATAAACTTGACTTCCAAATT+CGG 0.392960 3.4:-55953600 None:intergenic
GTACCGGAACGGGCGAATAT+TGG 0.396199 3.4:-55952035 None:intergenic
TCCACCTATTATTCTTGGAT+TGG 0.398159 3.4:+55952913 MS.gene04301:CDS
GGTGTAACACAAGAAATGAT+TGG 0.398343 3.4:+55952722 MS.gene04301:CDS
GCTATACCAAAATCACCTTC+TGG 0.399912 3.4:+55954720 MS.gene04301:CDS
AAGTTTATTGATCCTGAAAC+AGG 0.400350 3.4:+55953615 MS.gene04301:CDS
ACCTGAAAATGTAACATTGC+CGG 0.414752 3.4:+55952056 MS.gene04301:CDS
CATTATTGCTTTGGGAATCA+TGG 0.418941 3.4:+55952266 MS.gene04301:CDS
CTCAAAACAACACTTGCAGC+TGG 0.420863 3.4:-55954603 None:intergenic
TTGGAAAAGTCACTACATTA+GGG 0.421139 3.4:+55952741 MS.gene04301:CDS
CCTGTTGTGCCTGATGAAAA+TGG 0.423310 3.4:-55952635 None:intergenic
TCAAAGGCTTTAAGGTCTCT+TGG 0.427536 3.4:+55952189 MS.gene04301:CDS
AGAATAATAGGTGGAACAAT+AGG 0.427941 3.4:-55952905 None:intergenic
TGGTGATAAGGTTGCATTTG+TGG 0.435222 3.4:+55952107 MS.gene04301:CDS
TAGCCAATATTCGCCCGTTC+CGG 0.441770 3.4:+55952032 MS.gene04301:CDS
TAGAACAAAGATTTGCTACT+AGG 0.446233 3.4:-55952691 None:intergenic
AGATCAAATTGATCAACAAT+AGG 0.448219 3.4:-55952944 None:intergenic
TTTCACATTTATGGCATCAC+TGG 0.449930 3.4:+55952776 MS.gene04301:CDS
AGTGAGAGTGGTTCACTTTG+TGG 0.451776 3.4:+55954695 MS.gene04301:CDS
AACTTCTTGAAGCCGGAGAT+CGG 0.453268 3.4:+55952558 MS.gene04301:CDS
TTGATACAGGTGAAAAGTCT+AGG 0.459793 3.4:+55952479 MS.gene04301:intron
ACTTCTTGAAGCCGGAGATC+GGG 0.460564 3.4:+55952559 MS.gene04301:CDS
GGTTGCTCCTGCAGAGTTGG+AGG 0.467101 3.4:+55954247 MS.gene04301:intron
ATTTCCGACGTATGTGAAGC+TGG 0.470134 3.4:-55952318 None:intergenic
TTTCTTGTAATGAGCAGCAT+TGG 0.471695 3.4:-55954673 None:intergenic
CCCTGGTGTGTTCCTTGGGA+GGG 0.472351 3.4:-55953643 None:intergenic
TCCTTTGAACATAAGTTCCC+TGG 0.472567 3.4:+55953031 MS.gene04301:CDS
CTTCTCACACAAAGTTCCCC+TGG 0.483470 3.4:-55953660 None:intergenic
GATGCTGGTTGTGTGTTCTC+AGG 0.486269 3.4:+55952288 MS.gene04301:CDS
TGTGACTTCATGTGTAATCA+AGG 0.491977 3.4:-55952876 None:intergenic
ATCATTCCCTGCCCGATCTC+CGG 0.492090 3.4:-55952570 None:intergenic
CTTCAACAAGTTCATCTCCT+AGG 0.495549 3.4:-55952517 None:intergenic
GCAGGTTGCTCCTGCAGAGT+TGG 0.496551 3.4:+55954244 MS.gene04301:intron
CCCCTGGTGTGTTCCTTGGG+AGG 0.500432 3.4:-55953644 None:intergenic
TGCTGAACTATATGGTGATA+AGG 0.502771 3.4:+55952095 MS.gene04301:CDS
AGATTACCAGATGAAGAGGC+AGG 0.505717 3.4:+55954573 MS.gene04301:intron
AAATAGCTCAAGCCATGAAA+AGG 0.506415 3.4:+55952383 MS.gene04301:CDS
TTGTTGAAGATGCTATGAAC+TGG 0.506560 3.4:+55952531 MS.gene04301:CDS
GTGCAACTATTAGGAACAAA+GGG 0.513378 3.4:+55952807 MS.gene04301:CDS
GTGGATGCTGAGAGTGGAAA+AGG 0.515304 3.4:+55952126 MS.gene04301:CDS
GCTTTGGGAATCATGGATGC+TGG 0.516352 3.4:+55952273 MS.gene04301:CDS
CCCTGGTGTTCTTGTCCAAG+AGG 0.518092 3.4:+55953048 MS.gene04301:CDS
CTTGAAGCCGGAGATCGGGC+AGG 0.519508 3.4:+55952563 MS.gene04301:CDS
TAACTTATAGTGAGGTTGTG+AGG 0.520474 3.4:+55952151 MS.gene04301:CDS
GGGCTACCTGTGATAGTCCT+AGG 0.522982 3.4:+55952500 MS.gene04301:CDS
AAAATAGCCTCCAACTCTGC+AGG 0.523335 3.4:-55954254 None:intergenic
CAAAGGGAAAGTAGTGGTGA+TGG 0.526343 3.4:+55952823 MS.gene04301:CDS
ACCAGGGAACTTATGTTCAA+AGG 0.540093 3.4:-55953032 None:intergenic
AACTTATAGTGAGGTTGTGA+GGG 0.541554 3.4:+55952152 MS.gene04301:CDS
TGGCTTCACACTGGTGACAT+AGG 0.543428 3.4:+55953907 MS.gene04301:CDS
AAGGCTTGTCAAAGAGAAGA+TGG 0.550369 3.4:+55954755 MS.gene04301:CDS
TTACAGATTACCAGATGAAG+AGG 0.552721 3.4:+55954569 MS.gene04301:intron
ACCTCTTGGACAAGAACACC+AGG 0.557945 3.4:-55953049 None:intergenic
TCATCTCCTAGGACTATCAC+AGG 0.558388 3.4:-55952506 None:intergenic
TGGAACAAACTTCTTGAAGC+CGG 0.560308 3.4:+55952551 MS.gene04301:CDS
AAAGGGAAAGTAGTGGTGAT+GGG 0.563703 3.4:+55952824 MS.gene04301:CDS
GGCACAACAGGAATGTCAAA+GGG 0.563766 3.4:+55952647 MS.gene04301:CDS
TCAAAACAACACTTGCAGCT+GGG 0.565486 3.4:-55954602 None:intergenic
GGCAGGGAATGATTCAATGA+AGG 0.571367 3.4:+55952580 MS.gene04301:CDS
AGGCACAACAGGAATGTCAA+AGG 0.574039 3.4:+55952646 MS.gene04301:CDS
TCCCTCCCAAGGAACACACC+AGG 0.574638 3.4:+55953642 MS.gene04301:CDS
AAGGAGTTGATTAAATACAA+AGG 0.576483 3.4:+55953973 MS.gene04301:CDS
ACTTGACTTCCAAATTCGGA+AGG 0.585552 3.4:-55953596 None:intergenic
TGTGCAACTATTAGGAACAA+AGG 0.585600 3.4:+55952806 MS.gene04301:CDS
GATTACCAGATGAAGAGGCA+GGG 0.588955 3.4:+55954574 MS.gene04301:intron
TGATACAGGTGAAAAGTCTA+GGG 0.590073 3.4:+55952480 MS.gene04301:intron
CCCTCCCAAGGAACACACCA+GGG 0.599440 3.4:+55953643 MS.gene04301:CDS
TTGAAGCCGGAGATCGGGCA+GGG 0.599597 3.4:+55952564 MS.gene04301:CDS
ACTAGGTTATTATAAACAAG+TGG 0.600754 3.4:+55953852 MS.gene04301:intron
GATTACCTTTCATTACACAT+TGG 0.601358 3.4:-55953683 None:intergenic
ATTACCAGATGAAGAGGCAG+GGG 0.606768 3.4:+55954575 MS.gene04301:CDS
AAAAGGAGTAACTTATAGTG+AGG 0.610280 3.4:+55952143 MS.gene04301:CDS
GTAAGTAATTCAGGTGCAAG+TGG 0.616501 3.4:-55953007 None:intergenic
CTTCTGGTAAAATAATGAGA+AGG 0.618252 3.4:+55954736 MS.gene04301:CDS
ATGAAAATGGCATTGCACAA+AGG 0.621273 3.4:-55952622 None:intergenic
AACAGCTGCATCTTCAACTG+AGG 0.622182 3.4:-55954288 None:intergenic
GAACGGGCGAATATTGGCTA+CGG 0.624342 3.4:-55952029 None:intergenic
GCATTTGTGGATGCTGAGAG+TGG 0.629816 3.4:+55952120 MS.gene04301:CDS
ACCAATCCAAGAATAATAGG+TGG 0.632007 3.4:-55952914 None:intergenic
TAGGAACAAAGGGAAAGTAG+TGG 0.635216 3.4:+55952817 MS.gene04301:CDS
AGGTGCAAAAGAGAGTGAAG+AGG 0.641402 3.4:+55954629 MS.gene04301:CDS
AAACAGGAAGATCCCTCCCA+AGG 0.648638 3.4:+55953631 MS.gene04301:CDS
TCATTACAAGAAAGTGAGAG+TGG 0.654711 3.4:+55954683 MS.gene04301:CDS
AGAAGCCAATGTGTAATGAA+AGG 0.659864 3.4:+55953678 MS.gene04301:CDS
TAATCCAGCTTCACATACGT+CGG 0.679378 3.4:+55952314 MS.gene04301:CDS
CCTCCCAAGGAACACACCAG+GGG 0.693772 3.4:+55953644 MS.gene04301:CDS
GCTTGTCAAAGAGAAGATGG+TGG 0.703710 3.4:+55954758 MS.gene04301:CDS
AGGGTGTGATGCTTACACAC+AGG 0.727103 3.4:+55952666 MS.gene04301:CDS
CCTCTTGGACAAGAACACCA+GGG 0.754639 3.4:-55953048 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTTAGATAAATGTTA+GGG + chr3.4:55953733-55953752 MS.gene04301:intron 10.0%
!! TAATTATTTAGATAAATGTT+AGG + chr3.4:55953732-55953751 MS.gene04301:intron 10.0%
!! AATTATTTATGATCTTAATG+TGG + chr3.4:55954530-55954549 MS.gene04301:intron 15.0%
!! ATTATTTAGATAAATGTTAG+GGG + chr3.4:55953734-55953753 MS.gene04301:intron 15.0%
!!! AAAATTTGTTAGATTTTGTT+AGG - chr3.4:55954806-55954825 None:intergenic 15.0%
!!! AAATTTGTTAGATTTTGTTA+GGG - chr3.4:55954805-55954824 None:intergenic 15.0%
!!! GACTTTTTTTTTTTAGTATT+TGG + chr3.4:55953164-55953183 MS.gene04301:intron 15.0%
!!! TGAGTTTAATTTTAATATAC+CGG + chr3.4:55954382-55954401 MS.gene04301:intron 15.0%
!! GAGTACAATATTAGAATTTA+GGG - chr3.4:55953444-55953463 None:intergenic 20.0%
!! TGAACTAACAATAATATAGA+AGG - chr3.4:55953087-55953106 None:intergenic 20.0%
!! TGAGTACAATATTAGAATTT+AGG - chr3.4:55953445-55953464 None:intergenic 20.0%
!! TTATAATTATAACCCTTTGT+GGG - chr3.4:55953374-55953393 None:intergenic 20.0%
!! TTATTTAGATAAATGTTAGG+GGG + chr3.4:55953735-55953754 MS.gene04301:intron 20.0%
!! TTTATAATTATAACCCTTTG+TGG - chr3.4:55953375-55953394 None:intergenic 20.0%
!!! ATAATCTCTTTGTTTTTACT+AGG + chr3.4:55953835-55953854 MS.gene04301:intron 20.0%
!!! ATAATTATAAATACCCCTTT+TGG + chr3.4:55953384-55953403 MS.gene04301:intron 20.0%
!!! TAATTATAAATACCCCTTTT+GGG + chr3.4:55953385-55953404 MS.gene04301:intron 20.0%
!!! TTCTACAAAATTTTCTATGT+AGG - chr3.4:55951992-55952011 None:intergenic 20.0%
!!! TTTAATGTTACCTTTTCATT+GGG + chr3.4:55954350-55954369 MS.gene04301:intron 20.0%
!!! TTTTAATGTTACCTTTTCAT+TGG + chr3.4:55954349-55954368 MS.gene04301:intron 20.0%
!!! TTTTATTGTTGATCGTATTA+AGG + chr3.4:55953954-55953973 MS.gene04301:CDS 20.0%
! AATCTTAAATCCCAATGAAA+AGG - chr3.4:55954363-55954382 None:intergenic 25.0%
! ACATATGCACAAAAATTATC+TGG + chr3.4:55953543-55953562 MS.gene04301:CDS 25.0%
! ACTAGGTTATTATAAACAAG+TGG + chr3.4:55953852-55953871 MS.gene04301:intron 25.0%
! AGATCAAATTGATCAACAAT+AGG - chr3.4:55952947-55952966 None:intergenic 25.0%
! ATAAACTTGACTTCCAAATT+CGG - chr3.4:55953603-55953622 None:intergenic 25.0%
! ATCAACATTGTATCAAATCA+AGG - chr3.4:55952464-55952483 None:intergenic 25.0%
! ATGCCATAAATGTGAAAAAA+GGG - chr3.4:55952773-55952792 None:intergenic 25.0%
! ATGGAACAAAACATTATTTG+TGG + chr3.4:55954035-55954054 MS.gene04301:intron 25.0%
! ATTTGATACAATGTTGATAC+AGG + chr3.4:55952466-55952485 MS.gene04301:intron 25.0%
! GCTATTTGTGACAATTAATT+TGG - chr3.4:55952370-55952389 None:intergenic 25.0%
! GTTACATGTTTATTGTTACA+TGG + chr3.4:55954016-55954035 MS.gene04301:intron 25.0%
! TTAACCAATCCAAGAATAAT+AGG - chr3.4:55952920-55952939 None:intergenic 25.0%
!! AAGGAGTTGATTAAATACAA+AGG + chr3.4:55953973-55953992 MS.gene04301:CDS 25.0%
!! AATCAGACATTTTTAATCGA+CGG + chr3.4:55954478-55954497 MS.gene04301:intron 25.0%
!! ATAGATTTTCAAAGGCTTTA+AGG + chr3.4:55952181-55952200 MS.gene04301:CDS 25.0%
!! ATGATTCATCATCATTTTGA+AGG - chr3.4:55952414-55952433 None:intergenic 25.0%
!! GGATATACATAGATTTTCAA+AGG + chr3.4:55952173-55952192 MS.gene04301:CDS 25.0%
!! TGGAAGTACACATAAAAATT+CGG + chr3.4:55953563-55953582 MS.gene04301:CDS 25.0%
!!! AACTAATTTTAGCCTATACT+TGG - chr3.4:55954205-55954224 None:intergenic 25.0%
!!! ACTAATTTTAGCCTATACTT+GGG - chr3.4:55954204-55954223 None:intergenic 25.0%
!!! ATTCCCTTTTTTCACATTTA+TGG + chr3.4:55952767-55952786 MS.gene04301:CDS 25.0%
AAAAGGAGTAACTTATAGTG+AGG + chr3.4:55952143-55952162 MS.gene04301:CDS 30.0%
AAGTTTATTGATCCTGAAAC+AGG + chr3.4:55953615-55953634 MS.gene04301:CDS 30.0%
AATAACGCAAATTATTGTCG+CGG - chr3.4:55954327-55954346 None:intergenic 30.0%
AATCTTTGTTCTACACTCTT+TGG + chr3.4:55952701-55952720 MS.gene04301:CDS 30.0%
AGAATAATAGGTGGAACAAT+AGG - chr3.4:55952908-55952927 None:intergenic 30.0%
AGACTATTGACAAAAATGGA+TGG + chr3.4:55953887-55953906 MS.gene04301:CDS 30.0%
AGTACACATAAAAATTCGGT+TGG + chr3.4:55953567-55953586 MS.gene04301:CDS 30.0%
ATGGCATACTCTACAATATT+TGG - chr3.4:55952249-55952268 None:intergenic 30.0%
ATTATTAACAGCAATACCAC+TGG + chr3.4:55953766-55953785 MS.gene04301:intron 30.0%
ATTGGAAAAGTCACTACATT+AGG + chr3.4:55952740-55952759 MS.gene04301:CDS 30.0%
ATTGTTCCACCTATTATTCT+TGG + chr3.4:55952908-55952927 MS.gene04301:CDS 30.0%
ATTTGTGGAACATAATTGCA+AGG + chr3.4:55954050-55954069 MS.gene04301:intron 30.0%
CTTCTGGTAAAATAATGAGA+AGG + chr3.4:55954736-55954755 MS.gene04301:CDS 30.0%
GAATATGTTGTGCAACTATT+AGG + chr3.4:55952798-55952817 MS.gene04301:CDS 30.0%
GATGCCATAAATGTGAAAAA+AGG - chr3.4:55952774-55952793 None:intergenic 30.0%
GATTACCTTTCATTACACAT+TGG - chr3.4:55953686-55953705 None:intergenic 30.0%
TCAAAGGAAGTAAGTAATTC+AGG - chr3.4:55953019-55953038 None:intergenic 30.0%
TGTAACATCAAGAACTTACT+TGG - chr3.4:55954002-55954021 None:intergenic 30.0%
TTGGAAAAGTCACTACATTA+GGG + chr3.4:55952741-55952760 MS.gene04301:CDS 30.0%
! ATTTTACCAGAAGGTGATTT+TGG - chr3.4:55954729-55954748 None:intergenic 30.0%
! TCTAAAATCACACGTATGAT+TGG - chr3.4:55954443-55954462 None:intergenic 30.0%
!! AAATCAAGGAGTATCGAAAA+AGG - chr3.4:55952450-55952469 None:intergenic 30.0%
!! CTTCTCATTATTTTACCAGA+AGG - chr3.4:55954738-55954757 None:intergenic 30.0%
!! TAGAACAAAGATTTGCTACT+AGG - chr3.4:55952694-55952713 None:intergenic 30.0%
!!! AATGTTACATTTTCAGGTAC+CGG - chr3.4:55952054-55952073 None:intergenic 30.0%
!!! GTGTTTTCTTTTTTGAATGC+AGG + chr3.4:55954226-55954245 MS.gene04301:intron 30.0%
!!! TTTTTTCTTTTGCAGGCATA+TGG + chr3.4:55953492-55953511 MS.gene04301:intron 30.0%
AAATAGCTCAAGCCATGAAA+AGG + chr3.4:55952383-55952402 MS.gene04301:CDS 35.0%
AACTTATAGTGAGGTTGTGA+GGG + chr3.4:55952152-55952171 MS.gene04301:CDS 35.0%
ACCAATCCAAGAATAATAGG+TGG - chr3.4:55952917-55952936 None:intergenic 35.0%
ACCTGAAAATGTAACATTGC+CGG + chr3.4:55952056-55952075 MS.gene04301:CDS 35.0%
AGAAGCCAATGTGTAATGAA+AGG + chr3.4:55953678-55953697 MS.gene04301:CDS 35.0%
AGAGTATGCCATTATTGCTT+TGG + chr3.4:55952257-55952276 MS.gene04301:CDS 35.0%
ATGAAAATGGCATTGCACAA+AGG - chr3.4:55952625-55952644 None:intergenic 35.0%
ATTATTGTCGCGGTTACTTA+CGG - chr3.4:55954317-55954336 None:intergenic 35.0%
CATGATTCCCAAAGCAATAA+TGG - chr3.4:55952268-55952287 None:intergenic 35.0%
CTCCAAAATGCTGAACTATA+TGG + chr3.4:55952087-55952106 MS.gene04301:CDS 35.0%
GACCGTCAATATATTCCTAA+GGG + chr3.4:55953186-55953205 MS.gene04301:intron 35.0%
GAGTATGCCATTATTGCTTT+GGG + chr3.4:55952258-55952277 MS.gene04301:CDS 35.0%
GCTCAGACTATTGACAAAAA+TGG + chr3.4:55953883-55953902 MS.gene04301:CDS 35.0%
GGTGTAACACAAGAAATGAT+TGG + chr3.4:55952722-55952741 MS.gene04301:CDS 35.0%
GTGCAACTATTAGGAACAAA+GGG + chr3.4:55952807-55952826 MS.gene04301:CDS 35.0%
TAACTTATAGTGAGGTTGTG+AGG + chr3.4:55952151-55952170 MS.gene04301:CDS 35.0%
TAATATACCGGCAGTGTAAA+AGG + chr3.4:55954394-55954413 MS.gene04301:intron 35.0%
TCATTACAAGAAAGTGAGAG+TGG + chr3.4:55954683-55954702 MS.gene04301:CDS 35.0%
TCCACCTATTATTCTTGGAT+TGG + chr3.4:55952913-55952932 MS.gene04301:CDS 35.0%
TGATACAGGTGAAAAGTCTA+GGG + chr3.4:55952480-55952499 MS.gene04301:intron 35.0%
TGCTGAACTATATGGTGATA+AGG + chr3.4:55952095-55952114 MS.gene04301:CDS 35.0%
TGTGACTTCATGTGTAATCA+AGG - chr3.4:55952879-55952898 None:intergenic 35.0%
TGTGCAACTATTAGGAACAA+AGG + chr3.4:55952806-55952825 MS.gene04301:CDS 35.0%
TTACAGATTACCAGATGAAG+AGG + chr3.4:55954569-55954588 MS.gene04301:intron 35.0%
TTGATACAGGTGAAAAGTCT+AGG + chr3.4:55952479-55952498 MS.gene04301:intron 35.0%
TTGTTGAAGATGCTATGAAC+TGG + chr3.4:55952531-55952550 MS.gene04301:CDS 35.0%
TTTAGATAAATGTTAGGGGG+AGG + chr3.4:55953738-55953757 MS.gene04301:intron 35.0%
TTTCACATTTATGGCATCAC+TGG + chr3.4:55952776-55952795 MS.gene04301:CDS 35.0%
TTTCTTGTAATGAGCAGCAT+TGG - chr3.4:55954676-55954695 None:intergenic 35.0%
! ATTTTATCGTAAGTCTCCGA+AGG - chr3.4:55953798-55953817 None:intergenic 35.0%
! CACCATATAGTTCAGCATTT+TGG - chr3.4:55952092-55952111 None:intergenic 35.0%
! GAAATCTTTTGCCCAAGTAT+AGG + chr3.4:55954190-55954209 MS.gene04301:intron 35.0%
! TTGAAGGAGTTACCTTTTCA+TGG - chr3.4:55952398-55952417 None:intergenic 35.0%
!! CAAAGGTCATTTTGGTGAAT+CGG - chr3.4:55952608-55952627 None:intergenic 35.0%
!! CATTATTGCTTTGGGAATCA+TGG + chr3.4:55952266-55952285 MS.gene04301:CDS 35.0%
!! GAAGATGGTGGAAAAGATTA+AGG + chr3.4:55954770-55954789 MS.gene04301:CDS 35.0%
!!! GTGCTAGTTTTTTCTTTTGC+AGG + chr3.4:55953485-55953504 MS.gene04301:intron 35.0%
AAAAATGGATGGCTTCACAC+TGG + chr3.4:55953898-55953917 MS.gene04301:CDS 40.0%
AAAGGGAAAGTAGTGGTGAT+GGG + chr3.4:55952824-55952843 MS.gene04301:CDS 40.0%
AAGGCTTGTCAAAGAGAAGA+TGG + chr3.4:55954755-55954774 MS.gene04301:CDS 40.0%
ACCAGGGAACTTATGTTCAA+AGG - chr3.4:55953035-55953054 None:intergenic 40.0%
ACTTGACTTCCAAATTCGGA+AGG - chr3.4:55953599-55953618 None:intergenic 40.0%
CCTTGAGAATGATCCCAAAA+GGG - chr3.4:55953401-55953420 None:intergenic 40.0%
CTTCAACAAGTTCATCTCCT+AGG - chr3.4:55952520-55952539 None:intergenic 40.0%
CTTGAGAATGATCCCAAAAG+GGG - chr3.4:55953400-55953419 None:intergenic 40.0%
GCTATACCAAAATCACCTTC+TGG + chr3.4:55954720-55954739 MS.gene04301:CDS 40.0%
GGACCGTCAATATATTCCTA+AGG + chr3.4:55953185-55953204 MS.gene04301:intron 40.0%
GGATGAAAGTGAAAACCTCT+TGG - chr3.4:55953066-55953085 None:intergenic 40.0%
GGCCCTTAGGAATATATTGA+CGG - chr3.4:55953191-55953210 None:intergenic 40.0%
GTAAGTAATTCAGGTGCAAG+TGG - chr3.4:55953010-55953029 None:intergenic 40.0%
GTGTGTGTGTGTGTGAAAAT+AGG + chr3.4:55954104-55954123 MS.gene04301:intron 40.0%
TAATCCAGCTTCACATACGT+CGG + chr3.4:55952314-55952333 MS.gene04301:CDS 40.0%
TAGGAACAAAGGGAAAGTAG+TGG + chr3.4:55952817-55952836 MS.gene04301:CDS 40.0%
TCAAAACAACACTTGCAGCT+GGG - chr3.4:55954605-55954624 None:intergenic 40.0%
TCCTTTGAACATAAGTTCCC+TGG + chr3.4:55953031-55953050 MS.gene04301:CDS 40.0%
TGGAACAAACTTCTTGAAGC+CGG + chr3.4:55952551-55952570 MS.gene04301:CDS 40.0%
TGGTGATAAGGTTGCATTTG+TGG + chr3.4:55952107-55952126 MS.gene04301:CDS 40.0%
TGGTTTCATCCTTCCGAATT+TGG + chr3.4:55953587-55953606 MS.gene04301:CDS 40.0%
! AGGTCTCTTGGTTTGAGAAA+AGG + chr3.4:55952201-55952220 MS.gene04301:CDS 40.0%
! GAGCCTTAAGTCTTTTTACC+CGG - chr3.4:55953319-55953338 None:intergenic 40.0%
! GCATTGCACAAAGGTCATTT+TGG - chr3.4:55952616-55952635 None:intergenic 40.0%
! GGTCTCTTGGTTTGAGAAAA+GGG + chr3.4:55952202-55952221 MS.gene04301:CDS 40.0%
! GTATGATTGGTTGTGATCTG+TGG - chr3.4:55954430-55954449 None:intergenic 40.0%
! TCCGGCAATGTTACATTTTC+AGG - chr3.4:55952060-55952079 None:intergenic 40.0%
! TCTAGATGCTTCTAGAAGCT+GGG - chr3.4:55953295-55953314 None:intergenic 40.0%
! TGTTGAACCTTTTACACTGC+CGG - chr3.4:55954404-55954423 None:intergenic 40.0%
!! CTCTGAAATAAGCCCACAAA+GGG + chr3.4:55953359-55953378 MS.gene04301:intron 40.0%
!! TCAAAGGCTTTAAGGTCTCT+TGG + chr3.4:55952189-55952208 MS.gene04301:CDS 40.0%
!!! GCAAGTGTTGTTTTGAGCAA+AGG + chr3.4:55954609-55954628 MS.gene04301:CDS 40.0%
!!! GCATTTTGGAGCACAAATTC+CGG - chr3.4:55952078-55952097 None:intergenic 40.0%
!!! TACATTTTCAGGTACCGGAA+CGG - chr3.4:55952049-55952068 None:intergenic 40.0%
!!! TGTGCAATGCCATTTTCATC+AGG + chr3.4:55952626-55952645 MS.gene04301:CDS 40.0%
AAAATAGCCTCCAACTCTGC+AGG - chr3.4:55954257-55954276 None:intergenic 45.0%
AACAGCTGCATCTTCAACTG+AGG - chr3.4:55954291-55954310 None:intergenic 45.0%
AACTTCTTGAAGCCGGAGAT+CGG + chr3.4:55952558-55952577 MS.gene04301:CDS 45.0%
AGATTACCAGATGAAGAGGC+AGG + chr3.4:55954573-55954592 MS.gene04301:intron 45.0%
AGGCACAACAGGAATGTCAA+AGG + chr3.4:55952646-55952665 MS.gene04301:CDS 45.0%
AGGTGCAAAAGAGAGTGAAG+AGG + chr3.4:55954629-55954648 MS.gene04301:CDS 45.0%
AGTGAGAGTGGTTCACTTTG+TGG + chr3.4:55954695-55954714 MS.gene04301:CDS 45.0%
ATACCACTGGACTACACCTT+CGG + chr3.4:55953779-55953798 MS.gene04301:intron 45.0%
ATTACCAGATGAAGAGGCAG+GGG + chr3.4:55954575-55954594 MS.gene04301:CDS 45.0%
ATTTCCGACGTATGTGAAGC+TGG - chr3.4:55952321-55952340 None:intergenic 45.0%
CAAAGGGAAAGTAGTGGTGA+TGG + chr3.4:55952823-55952842 MS.gene04301:CDS 45.0%
CCTGTTGTGCCTGATGAAAA+TGG - chr3.4:55952638-55952657 None:intergenic 45.0%
CTCAAAACAACACTTGCAGC+TGG - chr3.4:55954606-55954625 None:intergenic 45.0%
GAGCCGGGTAAAAAGACTTA+AGG + chr3.4:55953313-55953332 MS.gene04301:intron 45.0%
GATTACCAGATGAAGAGGCA+GGG + chr3.4:55954574-55954593 MS.gene04301:intron 45.0%
GCCTTGAGAATGATCCCAAA+AGG - chr3.4:55953402-55953421 None:intergenic 45.0%
GCTTGTCAAAGAGAAGATGG+TGG + chr3.4:55954758-55954777 MS.gene04301:CDS 45.0%
GGCACAACAGGAATGTCAAA+GGG + chr3.4:55952647-55952666 MS.gene04301:CDS 45.0%
GGCAGGGAATGATTCAATGA+AGG + chr3.4:55952580-55952599 MS.gene04301:CDS 45.0%
TCATCTCCTAGGACTATCAC+AGG - chr3.4:55952509-55952528 None:intergenic 45.0%
! AAAGCCCTCTGGAAAGGTTT+AGG + chr3.4:55953213-55953232 MS.gene04301:intron 45.0%
! ATGCTTCTAGAAGCTGGGTA+AGG - chr3.4:55953290-55953309 None:intergenic 45.0%
! CCATTTTCATCAGGCACAAC+AGG + chr3.4:55952635-55952654 MS.gene04301:CDS 45.0%
! CTCTAGATGCTTCTAGAAGC+TGG - chr3.4:55953296-55953315 None:intergenic 45.0%
!! CTTCTAGAAGCATCTAGAGC+CGG + chr3.4:55953297-55953316 MS.gene04301:intron 45.0%
!! GCTCTGAAATAAGCCCACAA+AGG + chr3.4:55953358-55953377 MS.gene04301:intron 45.0%
!! TTCTAGAAGCATCTAGAGCC+GGG + chr3.4:55953298-55953317 MS.gene04301:intron 45.0%
!!! ACATTTTCAGGTACCGGAAC+GGG - chr3.4:55952048-55952067 None:intergenic 45.0%
!!! CCCTTTTGGGATCATTCTCA+AGG + chr3.4:55953398-55953417 MS.gene04301:intron 45.0%
AAACAGGAAGATCCCTCCCA+AGG + chr3.4:55953631-55953650 MS.gene04301:CDS 50.0%
AACCTTTCCAGAGGGCTTTC+AGG - chr3.4:55953212-55953231 None:intergenic 50.0%
ACCTCTTGGACAAGAACACC+AGG - chr3.4:55953052-55953071 None:intergenic 50.0%
ACTTCTTGAAGCCGGAGATC+GGG + chr3.4:55952559-55952578 MS.gene04301:CDS 50.0%
AGGGTGTGATGCTTACACAC+AGG + chr3.4:55952666-55952685 MS.gene04301:CDS 50.0%
ATTTCCCCTGCCTCTTCATC+TGG - chr3.4:55954582-55954601 None:intergenic 50.0%
CCTCTTGGACAAGAACACCA+GGG - chr3.4:55953051-55953070 None:intergenic 50.0%
CTTCTCACACAAAGTTCCCC+TGG - chr3.4:55953663-55953682 None:intergenic 50.0%
GCATTTGTGGATGCTGAGAG+TGG + chr3.4:55952120-55952139 MS.gene04301:CDS 50.0%
GTGGATGCTGAGAGTGGAAA+AGG + chr3.4:55952126-55952145 MS.gene04301:CDS 50.0%
TAGCCAATATTCGCCCGTTC+CGG + chr3.4:55952032-55952051 MS.gene04301:CDS 50.0%
TGGCTTCACACTGGTGACAT+AGG + chr3.4:55953907-55953926 MS.gene04301:CDS 50.0%
! CTTCTAGAAGCTGGGTAAGG+CGG - chr3.4:55953287-55953306 None:intergenic 50.0%
! GAACGGGCGAATATTGGCTA+CGG - chr3.4:55952032-55952051 None:intergenic 50.0%
! GACTTAAGGCTCAGCTTGCT+TGG + chr3.4:55953327-55953346 MS.gene04301:intron 50.0%
! GATGCTGGTTGTGTGTTCTC+AGG + chr3.4:55952288-55952307 MS.gene04301:CDS 50.0%
!! GCTTTGGGAATCATGGATGC+TGG + chr3.4:55952273-55952292 MS.gene04301:CDS 50.0%
AGTTCCCCTGGTGTGTTCCT+TGG - chr3.4:55953651-55953670 None:intergenic 55.0%
ATCATTCCCTGCCCGATCTC+CGG - chr3.4:55952573-55952592 None:intergenic 55.0%
CCCTGGTGTTCTTGTCCAAG+AGG + chr3.4:55953048-55953067 MS.gene04301:CDS 55.0%
GGCGCCTAAACCTTTCCAGA+GGG - chr3.4:55953220-55953239 None:intergenic 55.0%
GGGAGGGATCTTCCTGTTTC+AGG - chr3.4:55953630-55953649 None:intergenic 55.0%
GGGCTACCTGTGATAGTCCT+AGG + chr3.4:55952500-55952519 MS.gene04301:CDS 55.0%
GTACCGGAACGGGCGAATAT+TGG - chr3.4:55952038-55952057 None:intergenic 55.0%
GTTCCCCTGGTGTGTTCCTT+GGG - chr3.4:55953650-55953669 None:intergenic 55.0%
! TCTCCGAAGGTGTAGTCCAG+TGG - chr3.4:55953785-55953804 None:intergenic 55.0%
AGGTTTAGGCGCCCTCCAGA+AGG + chr3.4:55953227-55953246 MS.gene04301:intron 60.0%
CAGAAGGGCTCTTGAGGCCT+AGG + chr3.4:55953243-55953262 MS.gene04301:intron 60.0%
CAGAGGGCTTTCAGGCCCTT+AGG - chr3.4:55953204-55953223 None:intergenic 60.0%
CCCTCCCAAGGAACACACCA+GGG + chr3.4:55953643-55953662 MS.gene04301:CDS 60.0%
CCCTGGTGTGTTCCTTGGGA+GGG - chr3.4:55953646-55953665 None:intergenic 60.0%
CCTCCCAAGGAACACACCAG+GGG + chr3.4:55953644-55953663 MS.gene04301:CDS 60.0%
CTAAGGGCCTGAAAGCCCTC+TGG + chr3.4:55953202-55953221 MS.gene04301:intron 60.0%
CTAGGCCTCAAGAGCCCTTC+TGG - chr3.4:55953245-55953264 None:intergenic 60.0%
GCAGGTTGCTCCTGCAGAGT+TGG + chr3.4:55954244-55954263 MS.gene04301:intron 60.0%
GCCTCAAGAGCCCTTCTGGA+GGG - chr3.4:55953241-55953260 None:intergenic 60.0%
GGGCGCCTAAACCTTTCCAG+AGG - chr3.4:55953221-55953240 None:intergenic 60.0%
GGTTGCTCCTGCAGAGTTGG+AGG + chr3.4:55954247-55954266 MS.gene04301:intron 60.0%
GGTTTAGGCGCCCTCCAGAA+GGG + chr3.4:55953228-55953247 MS.gene04301:intron 60.0%
TCCCTCCCAAGGAACACACC+AGG + chr3.4:55953642-55953661 MS.gene04301:CDS 60.0%
TTGAAGCCGGAGATCGGGCA+GGG + chr3.4:55952564-55952583 MS.gene04301:CDS 60.0%
! GGCCTGAAAGCCCTCTGGAA+AGG + chr3.4:55953207-55953226 MS.gene04301:intron 60.0%
CCCCTGGTGTGTTCCTTGGG+AGG - chr3.4:55953647-55953666 None:intergenic 65.0%
CCTAGGCACGCCCTTCCAGA+GGG + chr3.4:55953260-55953279 MS.gene04301:intron 65.0%
CTAGGCACGCCCTTCCAGAG+GGG + chr3.4:55953261-55953280 MS.gene04301:intron 65.0%
CTTGAAGCCGGAGATCGGGC+AGG + chr3.4:55952563-55952582 MS.gene04301:CDS 65.0%
GCCCTCCAGAAGGGCTCTTG+AGG + chr3.4:55953237-55953256 MS.gene04301:intron 65.0%
GGCCTCAAGAGCCCTTCTGG+AGG - chr3.4:55953242-55953261 None:intergenic 65.0%
! GTAAGGCGGCCCCTCTGGAA+GGG - chr3.4:55953273-55953292 None:intergenic 65.0%
GCCTAGGCACGCCCTTCCAG+AGG + chr3.4:55953259-55953278 MS.gene04301:intron 70.0%
GGTAAGGCGGCCCCTCTGGA+AGG - chr3.4:55953274-55953293 None:intergenic 70.0%
! CCCTCTGGAAGGGCGTGCCT+AGG - chr3.4:55953263-55953282 None:intergenic 70.0%
GCTGGGTAAGGCGGCCCCTC+TGG - chr3.4:55953278-55953297 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 55951982 55954827 55951982 ID=MS.gene04301
chr3.4 mRNA 55951982 55954827 55951982 ID=MS.gene04301.t1;Parent=MS.gene04301
chr3.4 exon 55951982 55952404 55951982 ID=MS.gene04301.t1.exon1;Parent=MS.gene04301.t1
chr3.4 CDS 55951982 55952404 55951982 ID=cds.MS.gene04301.t1;Parent=MS.gene04301.t1
chr3.4 exon 55952488 55953069 55952488 ID=MS.gene04301.t1.exon2;Parent=MS.gene04301.t1
chr3.4 CDS 55952488 55953069 55952488 ID=cds.MS.gene04301.t1;Parent=MS.gene04301.t1
chr3.4 exon 55953507 55953699 55953507 ID=MS.gene04301.t1.exon3;Parent=MS.gene04301.t1
chr3.4 CDS 55953507 55953699 55953507 ID=cds.MS.gene04301.t1;Parent=MS.gene04301.t1
chr3.4 exon 55953857 55954002 55953857 ID=MS.gene04301.t1.exon4;Parent=MS.gene04301.t1
chr3.4 CDS 55953857 55954002 55953857 ID=cds.MS.gene04301.t1;Parent=MS.gene04301.t1
chr3.4 exon 55954248 55954315 55954248 ID=MS.gene04301.t1.exon5;Parent=MS.gene04301.t1
chr3.4 CDS 55954248 55954315 55954248 ID=cds.MS.gene04301.t1;Parent=MS.gene04301.t1
chr3.4 exon 55954575 55954827 55954575 ID=MS.gene04301.t1.exon6;Parent=MS.gene04301.t1
chr3.4 CDS 55954575 55954827 55954575 ID=cds.MS.gene04301.t1;Parent=MS.gene04301.t1
Gene Sequence

>MS.gene04301

ATGGGAGCCTACATAGAAAATTTTGTAGAAGAGAATGAACACATTTTCCGTAGCCAATATTCGCCCGTTCCGGTACCTGAAAATGTAACATTGCCGGAATTTGTGCTCCAAAATGCTGAACTATATGGTGATAAGGTTGCATTTGTGGATGCTGAGAGTGGAAAAGGAGTAACTTATAGTGAGGTTGTGAGGGATATACATAGATTTTCAAAGGCTTTAAGGTCTCTTGGTTTGAGAAAAGGGAATGTTGTGATTGTTGTACTTCCAAATATTGTAGAGTATGCCATTATTGCTTTGGGAATCATGGATGCTGGTTGTGTGTTCTCAGGAGCTAATCCAGCTTCACATACGTCGGAAATTAAGAAACAAGTTGAGTCTGCTGATGCCAAATTAATTGTCACAAATAGCTCAAGCCATGAAAAGGTAACTCCTTCAAAATGATGATGAATCATGTTTTTTCTTATGCCTTTTTCGATACTCCTTGATTTGATACAATGTTGATACAGGTGAAAAGTCTAGGGCTACCTGTGATAGTCCTAGGAGATGAACTTGTTGAAGATGCTATGAACTGGAACAAACTTCTTGAAGCCGGAGATCGGGCAGGGAATGATTCAATGAAGGAACCGATTCACCAAAATGACCTTTGTGCAATGCCATTTTCATCAGGCACAACAGGAATGTCAAAGGGTGTGATGCTTACACACAGGAACCTAGTAGCAAATCTTTGTTCTACACTCTTTGGTGTAACACAAGAAATGATTGGAAAAGTCACTACATTAGGGTTAATTCCCTTTTTTCACATTTATGGCATCACTGGAATATGTTGTGCAACTATTAGGAACAAAGGGAAAGTAGTGGTGATGGGAAAATATGATTTGAAAACATTTTTGAATGCCTTGATTACACATGAAGTCACATTTGCACCTATTGTTCCACCTATTATTCTTGGATTGGTTAAAAATCCTATTGTTGATCAATTTGATCTTAGTAAGCTTAAACTTCAAGCTATTATGACTGCTGCTGCACCACTTGCACCTGAATTACTTACTTCCTTTGAACATAAGTTCCCTGGTGTTCTTGTCCAAGAGGTTTTCACTTTCATCCTTCTATATTATTGTTAGTTCAATTACAGACTTTTTTAGAGTTTGTGAAAATATTTTCTTAAACTATTACATAGTTAAAGACTTTTTTTTTTTAGTATTTGGACCGTCAATATATTCCTAAGGGCCTGAAAGCCCTCTGGAAAGGTTTAGGCGCCCTCCAGAAGGGCTCTTGAGGCCTAGGCACGCCCTTCCAGAGGGGCCGCCTTACCCAGCTTCTAGAAGCATCTAGAGCCGGGTAAAAAGACTTAAGGCTCAGCTTGCTTGGCTTAAGTCGCTCTGAAATAAGCCCACAAAGGGTTATAATTATAAATACCCCTTTTGGGATCATTCTCAAGGCATCAAATAAACAGTCTAAACCCTAAATTCTAATATTGTACTCAGTACAGTATGTGTTTCTAAAGTGCTAGTTTTTTCTTTTGCAGGCATATGGACTAACTGAGCATAGCTGCATCACACTTACATATGCACAAAAATTATCTGGAAGTACACATAAAAATTCGGTTGGTTTCATCCTTCCGAATTTGGAAGTCAAGTTTATTGATCCTGAAACAGGAAGATCCCTCCCAAGGAACACACCAGGGGAACTTTGTGTGAGAAGCCAATGTGTAATGAAAGGTAATCTATGAGTAAAATTATTTATGAAAATATAATTATTTAGATAAATGTTAGGGGGAGGCGAAAATTATTAACAGCAATACCACTGGACTACACCTTCGGAGACTTACGATAAAATTATATGATAAATGTACTATAATCTCTTTGTTTTTACTAGGTTATTATAAACAAGTGGATGAGACTGCTCAGACTATTGACAAAAATGGATGGCTTCACACTGGTGACATAGGATTCATAGACGATGAAGAAAATGTTTTTATTGTTGATCGTATTAAGGAGTTGATTAAATACAAAGGCTTCCAAGTAAGTTCTTGATGTTACATGTTTATTGTTACATGGAACAAAACATTATTTGTGGAACATAATTGCAAGGTCTTAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAAAATAGGAGAAATCAAATTTGTAATGCAAGTTCTGTTTTGTAGTTTATTAATATGCATCTATAAGTCTAAGAAATCTTTTGCCCAAGTATAGGCTAAAATTAGTTTGTGTTTTCTTTTTTGAATGCAGGTTGCTCCTGCAGAGTTGGAGGCTATTTTGCTGTCTCACTCCTCAGTTGAAGATGCAGCTGTTGTACCGTAAGTAACCGCGACAATAATTTGCGTTATTCTTTTTAATGTTACCTTTTCATTGGGATTTAAGATTGAGTTTAATTTTAATATACCGGCAGTGTAAAAGGTTCAACATATCCACAGATCACAACCAATCATACGTGTGATTTTAGAAATAGTTACGATTAAAATCAGACATTTTTAATCGACGGTCTATACGAATTAAATCTTACAAGATTTCAATTATTTATGATCTTAATGTGGTTTTTTGCAATGATTGTTACAGATTACCAGATGAAGAGGCAGGGGAAATCCCAGCTGCAAGTGTTGTTTTGAGCAAAGGTGCAAAAGAGAGTGAAGAGGACATTATGAACTATGTTGCATCCAATGCTGCTCATTACAAGAAAGTGAGAGTGGTTCACTTTGTGGAAGCTATACCAAAATCACCTTCTGGTAAAATAATGAGAAGGCTTGTCAAAGAGAAGATGGTGGAAAAGATTAAGGAAAATAATTCCCTAACAAAATCTAACAAATTTTAA
Protein sequence

>MS.gene04301.t1

MGAYIENFVEENEHIFRSQYSPVPVPENVTLPEFVLQNAELYGDKVAFVDAESGKGVTYSEVVRDIHRFSKALRSLGLRKGNVVIVVLPNIVEYAIIALGIMDAGCVFSGANPASHTSEIKKQVESADAKLIVTNSSSHEKVKSLGLPVIVLGDELVEDAMNWNKLLEAGDRAGNDSMKEPIHQNDLCAMPFSSGTTGMSKGVMLTHRNLVANLCSTLFGVTQEMIGKVTTLGLIPFFHIYGITGICCATIRNKGKVVVMGKYDLKTFLNALITHEVTFAPIVPPIILGLVKNPIVDQFDLSKLKLQAIMTAAAPLAPELLTSFEHKFPGVLVQEAYGLTEHSCITLTYAQKLSGSTHKNSVGFILPNLEVKFIDPETGRSLPRNTPGELCVRSQCVMKGYYKQVDETAQTIDKNGWLHTGDIGFIDDEENVFIVDRIKELIKYKGFQVAPAELEAILLSHSSVEDAAVVPLPDEEAGEIPAASVVLSKGAKESEEDIMNYVASNAAHYKKVRVVHFVEAIPKSPSGKIMRRLVKEKMVEKIKENNSLTKSNKF