AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27109

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27109.t1 MTR_1g026310 95.536 560 18 1 1 553 1 560 0.0 1098
MS.gene27109.t1 MTR_8g039720 80.071 567 91 6 1 553 1 559 0.0 908
MS.gene27109.t1 MTR_1g026470 94.802 404 14 1 1 397 1 404 0.0 781
MS.gene27109.t1 MTR_1g026430 95.511 401 11 1 1 394 1 401 0.0 780
MS.gene27109.t1 MTR_4g057730 45.672 543 286 5 17 553 3 542 5.40e-162 472
MS.gene27109.t1 MTR_3g088870 45.307 554 270 11 14 549 16 554 8.62e-160 467
MS.gene27109.t1 MTR_5g027480 43.750 560 303 6 1 553 1 555 1.14e-158 464
MS.gene27109.t1 MTR_1g090737 46.875 544 271 8 15 546 22 559 4.28e-158 463
MS.gene27109.t1 MTR_3g088880 44.464 560 277 12 7 549 10 552 3.40e-157 461
MS.gene27109.t1 MTR_2g095510 43.693 547 288 6 17 553 3 539 4.28e-153 449
MS.gene27109.t1 MTR_5g027500 44.973 547 277 9 26 553 27 568 3.33e-152 448
MS.gene27109.t1 MTR_4g077900 46.473 482 246 5 80 553 1 478 2.06e-151 443
MS.gene27109.t1 MTR_1g063110 43.808 541 288 8 27 553 13 551 6.98e-145 429
MS.gene27109.t1 MTR_1g063120 43.486 545 288 8 27 553 13 555 3.74e-143 424
MS.gene27109.t1 MTR_1g073180 43.808 541 282 9 14 546 1 527 1.29e-139 415
MS.gene27109.t1 MTR_4g057730 44.068 472 255 5 17 482 3 471 3.08e-130 389
MS.gene27109.t1 MTR_1g073180 42.533 529 279 10 8 528 20 531 9.37e-126 379
MS.gene27109.t1 MTR_2g105570 40.262 534 301 8 26 545 12 541 1.30e-120 367
MS.gene27109.t1 MTR_4g057730 43.111 450 247 5 17 460 3 449 5.95e-119 359
MS.gene27109.t1 MTR_4g128337 38.593 526 315 4 26 545 17 540 3.82e-118 360
MS.gene27109.t1 MTR_3g460770 35.261 536 323 7 20 543 8 531 2.02e-116 356
MS.gene27109.t1 MTR_5g007640 39.212 533 303 8 26 545 55 579 3.46e-116 356
MS.gene27109.t1 MTR_4g005750 40.341 528 303 8 26 545 11 534 6.74e-114 349
MS.gene27109.t1 MTR_2g095510 40.749 454 249 6 17 460 3 446 2.90e-108 332
MS.gene27109.t1 MTR_1g026350 94.118 153 9 0 1 153 1 153 9.06e-101 302
MS.gene27109.t1 MTR_1569s0010 27.885 520 319 11 68 545 22 527 1.34e-54 193
MS.gene27109.t1 MTR_3g035130 28.880 509 335 13 44 543 15 505 6.88e-52 185
MS.gene27109.t1 MTR_8g074040 27.843 510 317 15 67 542 81 573 8.60e-47 173
MS.gene27109.t1 MTR_3g064420 27.308 520 329 13 65 545 37 546 9.76e-46 169
MS.gene27109.t1 MTR_5g008800 27.621 496 326 12 68 539 40 526 1.01e-43 163
MS.gene27109.t1 MTR_3g035130 29.167 456 296 13 44 490 15 452 4.28e-42 157
MS.gene27109.t1 MTR_5g076240 26.705 528 318 14 68 549 40 544 2.84e-41 157
MS.gene27109.t1 MTR_8g074040 27.983 461 281 15 116 542 12 455 2.22e-40 153
MS.gene27109.t1 MTR_4g013210 29.563 389 238 11 192 552 65 445 2.98e-39 149
MS.gene27109.t1 MTR_5g008820 25.605 496 334 13 68 539 40 524 7.29e-39 150
MS.gene27109.t1 MTR_0803s0020 25.979 485 337 11 67 539 260 734 1.30e-38 152
MS.gene27109.t1 MTR_8g074120 27.101 476 302 15 98 542 23 484 3.30e-37 144
MS.gene27109.t1 MTR_2g044180 24.723 542 359 17 49 553 29 558 1.32e-36 144
MS.gene27109.t1 MTR_5g076220 25.528 521 334 11 68 549 40 545 2.00e-36 143
MS.gene27109.t1 MTR_4g011790 26.440 382 246 9 197 552 226 598 2.15e-36 143
MS.gene27109.t1 MTR_5g076200 25.434 519 337 11 68 549 40 545 2.31e-36 143
MS.gene27109.t1 MTR_4g121600 24.670 531 347 14 67 553 45 566 5.58e-35 139
MS.gene27109.t1 MTR_0161s0050 26.232 568 355 16 24 549 2 547 1.45e-34 137
MS.gene27109.t1 MTR_4g011790 27.110 391 241 12 197 552 226 607 1.83e-34 138
MS.gene27109.t1 MTR_4g011790 25.946 370 239 9 197 540 226 586 1.03e-33 135
MS.gene27109.t1 MTR_7g084850 23.092 524 363 11 68 553 203 724 1.32e-33 136
MS.gene27109.t1 MTR_2g044180 27.660 376 246 12 195 553 101 467 2.57e-33 133
MS.gene27109.t1 MTR_4g013210 24.854 515 326 17 52 521 68 566 6.57e-33 133
MS.gene27109.t1 MTR_5g023730 24.803 508 334 16 67 542 42 533 7.76e-32 129
MS.gene27109.t1 MTR_4g013210 29.521 376 214 14 192 552 65 404 2.04e-31 126
MS.gene27109.t1 MTR_4g013210 25.612 531 323 21 52 552 68 556 6.18e-31 127
MS.gene27109.t1 MTR_8g083170 25.919 571 301 18 69 528 110 669 1.33e-29 124
MS.gene27109.t1 MTR_3g084470 26.177 531 274 18 69 491 106 626 2.35e-28 120
MS.gene27109.t1 MTR_2g030240 25.282 443 299 11 69 485 29 465 1.51e-26 114
MS.gene27109.t1 MTR_2g030240 27.243 301 196 7 202 485 77 371 5.99e-24 105
MS.gene27109.t1 MTR_3g088890 60.938 64 25 0 455 518 10 73 1.67e-22 92.4
MS.gene27109.t1 MTR_1g104810 25.109 458 256 16 62 466 141 564 1.60e-21 99.0
MS.gene27109.t1 MTR_1g104810 25.328 458 255 15 62 466 74 497 2.47e-21 98.2
MS.gene27109.t1 MTR_4g057755 57.534 73 31 0 409 481 46 118 2.81e-21 90.1
MS.gene27109.t1 MTR_1054s0020 24.048 499 337 19 65 547 294 766 3.09e-21 98.6
MS.gene27109.t1 MTR_3g087980 27.697 343 174 15 198 475 248 581 4.18e-20 94.7
MS.gene27109.t1 MTR_1g086600 25.749 334 192 11 198 480 243 571 1.25e-19 93.2
MS.gene27109.t1 MTR_1g030290 28.235 340 176 13 198 475 250 583 2.38e-19 92.4
MS.gene27109.t1 MTR_4g066130 25.734 443 268 17 158 552 619 1048 5.56e-19 92.0
MS.gene27109.t1 MTR_2g098490 24.943 441 266 17 158 547 614 1040 9.29e-19 91.3
MS.gene27109.t1 MTR_8g099420 24.850 334 187 12 196 475 222 545 2.72e-18 89.0
MS.gene27109.t1 MTR_4g013210 24.429 438 271 18 52 470 68 464 8.97e-17 83.6
MS.gene27109.t1 MTR_3g084450 33.503 197 114 5 322 505 246 438 1.37e-16 83.2
MS.gene27109.t1 MTR_3g084450 33.503 197 114 5 322 505 454 646 3.23e-16 82.4
MS.gene27109.t1 MTR_7g109830 34.756 164 99 6 321 480 388 547 5.69e-15 78.6
MS.gene27109.t1 MTR_4g068670 22.626 495 295 19 62 485 72 549 2.55e-14 76.3
MS.gene27109.t1 MTR_5g009350 22.659 331 200 10 195 475 220 544 1.00e-13 74.3
MS.gene27109.t1 MTR_5g009350 22.659 331 200 10 195 475 220 544 1.02e-13 74.3
MS.gene27109.t1 MTR_5g009350 23.030 330 198 10 196 475 221 544 1.19e-13 74.3
MS.gene27109.t1 MTR_5g009350 21.905 420 250 14 196 546 221 631 1.24e-13 74.3
MS.gene27109.t1 MTR_4g013210 26.613 248 151 9 192 419 217 453 1.80e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27109.t1 AT1G20510 74.630 540 134 2 14 551 4 542 0.0 828
MS.gene27109.t1 AT1G20500 66.302 549 170 5 13 553 9 550 0.0 738
MS.gene27109.t1 AT1G20510 73.673 471 121 2 14 482 4 473 0.0 711
MS.gene27109.t1 AT1G20480 61.429 560 202 4 4 553 10 565 0.0 708
MS.gene27109.t1 AT5G38120 61.580 557 193 7 9 553 3 550 0.0 693
MS.gene27109.t1 AT1G20510 73.051 449 118 2 14 460 4 451 0.0 675
MS.gene27109.t1 AT5G38120 60.269 521 186 7 9 517 3 514 0.0 635
MS.gene27109.t1 AT5G38120 60.494 486 171 7 9 482 3 479 0.0 590
MS.gene27109.t1 AT5G38120 60.494 486 171 7 9 482 3 479 0.0 590
MS.gene27109.t1 AT1G20490 60.047 428 154 5 13 430 9 429 1.85e-179 513
MS.gene27109.t1 AT1G20490 60.047 428 154 5 13 430 9 429 1.85e-179 513
MS.gene27109.t1 AT5G63380 43.162 563 286 8 8 549 5 554 2.75e-165 481
MS.gene27109.t1 AT4G19010 41.230 553 301 6 15 553 20 562 5.85e-156 457
MS.gene27109.t1 AT4G05160 44.059 547 291 7 17 553 3 544 1.28e-155 456
MS.gene27109.t1 AT3G21240 39.662 532 312 6 21 545 17 546 3.51e-121 368
MS.gene27109.t1 AT1G65060 40.832 529 298 6 26 545 34 556 1.92e-120 367
MS.gene27109.t1 AT1G51680 39.560 546 316 6 12 545 10 553 8.28e-113 347
MS.gene27109.t1 AT3G21230 38.817 541 310 9 22 545 24 560 4.25e-111 343
MS.gene27109.t1 AT1G62940 35.810 525 328 4 26 545 12 532 6.96e-103 320
MS.gene27109.t1 AT3G21230 37.308 520 301 10 22 524 24 535 6.67e-98 308
MS.gene27109.t1 AT1G51680 37.645 518 309 6 12 517 10 525 7.78e-98 307
MS.gene27109.t1 AT3G21240 37.712 472 285 6 21 485 17 486 1.55e-96 302
MS.gene27109.t1 AT3G21240 37.712 472 285 6 21 485 17 486 1.55e-96 302
MS.gene27109.t1 AT3G21230 37.317 477 278 9 22 481 24 496 7.92e-89 283
MS.gene27109.t1 AT1G51680 37.344 482 288 6 12 481 10 489 3.74e-88 281
MS.gene27109.t1 AT1G65060 37.444 446 264 6 26 462 34 473 9.74e-82 264
MS.gene27109.t1 AT3G21230 32.924 407 252 9 22 411 24 426 3.06e-52 184
MS.gene27109.t1 AT3G48990 28.008 482 335 10 67 543 30 504 1.41e-47 174
MS.gene27109.t1 AT1G65890 28.060 531 317 18 67 549 39 552 1.21e-46 172
MS.gene27109.t1 AT1G21530 27.514 527 323 15 62 549 37 543 3.02e-46 171
MS.gene27109.t1 AT1G20560 27.188 537 348 14 34 542 14 535 5.32e-46 170
MS.gene27109.t1 AT1G21530 27.238 525 329 14 62 549 37 545 1.00e-45 169
MS.gene27109.t1 AT1G77240 26.515 528 338 15 58 551 32 543 3.08e-45 167
MS.gene27109.t1 AT1G65880 28.839 534 310 20 67 549 39 553 3.83e-45 168
MS.gene27109.t1 AT1G21540 27.746 519 323 14 69 551 46 548 1.86e-44 166
MS.gene27109.t1 AT1G76290 28.101 516 318 18 67 549 39 534 2.41e-44 165
MS.gene27109.t1 AT1G66120 27.810 525 323 15 67 551 39 547 5.93e-44 164
MS.gene27109.t1 AT5G16370 26.628 522 326 14 74 553 43 549 1.98e-41 157
MS.gene27109.t1 AT2G17650 27.321 560 343 20 37 549 55 597 3.16e-40 154
MS.gene27109.t1 AT5G16340 26.796 515 321 14 74 547 43 542 1.61e-39 152
MS.gene27109.t1 AT1G20560 27.692 455 292 12 116 542 12 457 3.41e-39 149
MS.gene27109.t1 AT1G30520 27.155 464 292 11 64 496 34 482 8.02e-38 147
MS.gene27109.t1 AT1G30520 27.155 464 292 11 64 496 34 482 8.02e-38 147
MS.gene27109.t1 AT1G75960 26.488 521 324 14 68 549 41 541 8.76e-37 144
MS.gene27109.t1 AT1G68270 24.952 521 308 16 67 545 39 518 2.41e-33 134
MS.gene27109.t1 AT3G16170 24.672 381 245 9 197 547 234 602 3.24e-32 131
MS.gene27109.t1 AT5G36880 22.761 536 362 13 60 553 144 669 3.29e-32 132
MS.gene27109.t1 AT5G36880 22.761 536 362 13 60 553 144 669 3.29e-32 132
MS.gene27109.t1 AT5G36880 22.761 536 362 13 60 553 144 669 3.29e-32 132
MS.gene27109.t1 AT5G36880 22.761 536 362 13 60 553 144 669 3.29e-32 132
MS.gene27109.t1 AT5G36880 22.948 536 361 14 60 553 194 719 4.51e-32 131
MS.gene27109.t1 AT3G16910 23.654 520 344 16 67 545 47 554 1.01e-31 129
MS.gene27109.t1 AT1G30520 25.843 445 281 12 64 477 34 460 1.49e-30 125
MS.gene27109.t1 AT1G30520 25.843 445 281 12 64 477 34 460 1.49e-30 125
MS.gene27109.t1 AT5G36880 21.957 460 312 11 60 481 144 594 3.60e-25 110
MS.gene27109.t1 AT5G27600 27.760 317 172 9 197 466 261 567 2.10e-22 102
MS.gene27109.t1 AT3G05970 28.707 317 169 9 197 466 261 567 5.32e-20 94.4
MS.gene27109.t1 AT1G64400 22.515 493 303 19 55 478 65 547 1.58e-19 92.8
MS.gene27109.t1 AT1G77590 27.874 348 167 14 198 475 100 433 2.89e-19 91.7
MS.gene27109.t1 AT1G77590 27.874 348 167 14 198 475 246 579 5.43e-19 91.3
MS.gene27109.t1 AT2G47240 26.571 350 200 12 180 480 147 488 6.39e-19 90.9
MS.gene27109.t1 AT2G47240 26.571 350 200 12 180 480 206 547 7.16e-19 90.9
MS.gene27109.t1 AT2G47240 26.571 350 200 12 180 480 206 547 7.16e-19 90.9
MS.gene27109.t1 AT2G47240 26.571 350 200 12 180 480 206 547 7.16e-19 90.9
MS.gene27109.t1 AT1G49430 25.000 344 199 13 193 485 219 554 1.01e-16 84.0
MS.gene27109.t1 AT4G11030 23.548 310 174 10 198 454 224 523 7.07e-15 78.2
MS.gene27109.t1 AT3G23790 32.632 190 103 6 322 490 455 640 4.13e-13 72.8
MS.gene27109.t1 AT3G23790 24.797 246 152 9 68 282 111 354 2.54e-12 70.1
MS.gene27109.t1 AT4G14070 29.600 250 145 8 322 546 471 714 5.61e-12 68.9

Find 158 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGCCCAGCCTCCTTATT+TGG 0.136723 1.1:-138009 None:intergenic
TCGCTTCATACGGTGGTTTC+TGG 0.138183 1.1:+136201 MS.gene27109:CDS
CTGCTGCAAAACCCACTTCT+TGG 0.251921 1.1:-135298 None:intergenic
ACCACTTGTGCCGCCGATAC+TGG 0.271545 1.1:+136128 MS.gene27109:CDS
GTAAGTATCTTTCAAGGTTA+TGG 0.275288 1.1:+136279 MS.gene27109:CDS
TGGAGCTTCTACTGAGTCTT+TGG 0.282200 1.1:+136323 MS.gene27109:CDS
TGTTGTTGATGATGAGTTGC+TGG 0.335506 1.1:-135755 None:intergenic
GTTCCGATGTTTCATATATA+CGG 0.342157 1.1:+135991 MS.gene27109:CDS
TGCTATGGGGCTTCTTGCTA+TGG 0.342915 1.1:+136026 MS.gene27109:CDS
TTAACTGAATCCTCTGGAAT+TGG 0.351281 1.1:+136303 MS.gene27109:CDS
AGCTCACCAGTCCGATTAAC+TGG 0.354985 1.1:-136432 None:intergenic
CTCTACTCATCAGGAACAAC+TGG 0.359656 1.1:+135859 MS.gene27109:CDS
CAAGTTGTAATGGCTAGATT+TGG 0.372468 1.1:+135931 MS.gene27109:CDS
GGAGGATGACGTCACCTTTA+CGG 0.372564 1.1:-135519 None:intergenic
TTATATCAGGTATCCAAATA+AGG 0.373402 1.1:+137996 MS.gene27109:intron
CATTAAGTACAAGGGATATC+AGG 0.379179 1.1:+137578 MS.gene27109:CDS
GGTGAATTGGTGGCCGGTGG+AGG 0.402061 1.1:-135437 None:intergenic
ATGACGGAGAGGCAGACAAC+AGG 0.402334 1.1:-135568 None:intergenic
AAGGCTGGAAGTAGCTTGTC+AGG 0.410549 1.1:+138054 MS.gene27109:CDS
TTAATCGGACTGGTGAGCTT+TGG 0.414553 1.1:+136436 MS.gene27109:CDS
TGGAGTTGGGAGAGAGGAGG+AGG 0.419067 1.1:-135537 None:intergenic
GAATTTAGAGAGAACAACTA+TGG 0.419281 1.1:-136055 None:intergenic
AAGAAACGAACCTTTCATAA+TGG 0.419398 1.1:-136472 None:intergenic
AGTAGATGGAGTTGGGAGAG+AGG 0.420482 1.1:-135543 None:intergenic
TGGTTTGGTGGAAGTGGAAG+GGG 0.421746 1.1:-135349 None:intergenic
ACTGGTGAGCTTTGGCTTCG+TGG 0.421933 1.1:+136444 MS.gene27109:CDS
AGTGAAAGCAAGAACAGGTT+TGG 0.423538 1.1:-135665 None:intergenic
ATCCATCTGGTAAAATTCTT+CGG 0.423771 1.1:+138273 MS.gene27109:CDS
AAAGAGTGGTTTGGTGGAAG+TGG 0.424298 1.1:-135355 None:intergenic
CCTTGAAAGATACTTACATT+AGG 0.428705 1.1:-136273 None:intergenic
TCCAAATAAGGAGGCTGGGC+AGG 0.431867 1.1:+138008 MS.gene27109:CDS
CCTAATGTAAGTATCTTTCA+AGG 0.432845 1.1:+136273 MS.gene27109:CDS
CTCAGCCGTTACCAGTTAAT+CGG 0.436385 1.1:+136421 MS.gene27109:CDS
GGTCTTGCAGTGTTTGCTAT+GGG 0.437424 1.1:+136012 MS.gene27109:CDS
CGGTCTTGCAGTGTTTGCTA+TGG 0.438320 1.1:+136011 MS.gene27109:CDS
GCTATGGGGCTTCTTGCTAT+GGG 0.440856 1.1:+136027 MS.gene27109:CDS
CTACAGCACGCCAGAGTTGT+TGG 0.441513 1.1:-135462 None:intergenic
GCAGACAACAGGGAAGTAGA+TGG 0.455653 1.1:-135557 None:intergenic
CTTGTCAGGAAGCCAAGTTA+TGG 0.458124 1.1:+138068 MS.gene27109:CDS
TTGGAAGAGAGTCGTAAGTA+TGG 0.463115 1.1:+136342 MS.gene27109:CDS
AGGCGTCAATGAAGGCGATA+TGG 0.463838 1.1:-135417 None:intergenic
GGGGCTTGTGTCTGCTTCCA+CGG 0.471317 1.1:+136371 MS.gene27109:CDS
TATGGTTTAACTGAATCCTC+TGG 0.471540 1.1:+136297 MS.gene27109:CDS
GTGGTTTGGTGGAAGTGGAA+GGG 0.473586 1.1:-135350 None:intergenic
AGAGTTGTTGGTAGGTGAAT+TGG 0.474858 1.1:-135450 None:intergenic
AGGTATCCAAATAAGGAGGC+TGG 0.481674 1.1:+138003 MS.gene27109:intron
TTCACTTCCAATCATTCTCA+TGG 0.482486 1.1:+135726 MS.gene27109:CDS
TAACCAACCCTCTGGAGTAA+TGG 0.484888 1.1:-137487 None:intergenic
CGATTTCGCGAGATGTATTG+AGG 0.485277 1.1:-135624 None:intergenic
GCGAGATGTATTGAGGGGAT+TGG 0.486320 1.1:-135617 None:intergenic
AACAAAGCCTCTAGTTCTGC+TGG 0.486762 1.1:-137789 None:intergenic
CACTTCCTCACTCTCCAACA+TGG 0.488973 1.1:+135492 MS.gene27109:CDS
GCCGGTGGAGGCGTCAATGA+AGG 0.490250 1.1:-135425 None:intergenic
AGTGGTTTGGTGGAAGTGGA+AGG 0.490859 1.1:-135351 None:intergenic
GCATATGTGGTAAGGAAGGC+TGG 0.491572 1.1:+138039 MS.gene27109:CDS
ATTAAGAAGATGGAGGTAAA+CGG 0.492454 1.1:+135244 None:intergenic
TGTATTGAGGGGATTGGTGG+TGG 0.493354 1.1:-135611 None:intergenic
CGATGGATTCTTATTTGTCG+TGG 0.494381 1.1:+137539 MS.gene27109:CDS
GTGGTGTCCATGAGAATGAT+TGG 0.496058 1.1:-135733 None:intergenic
CTGCAACAAAATCCATAACT+TGG 0.502133 1.1:-138080 None:intergenic
ACAGGGAAGTAGATGGAGTT+GGG 0.504299 1.1:-135550 None:intergenic
TGATTTGAGTTCGCTTCATA+CGG 0.505643 1.1:+136191 MS.gene27109:CDS
TTGGTAGGTGAATTGGTGGC+CGG 0.506146 1.1:-135443 None:intergenic
GCCACACTACTCTACTCATC+AGG 0.506955 1.1:+135850 MS.gene27109:CDS
AGTCGTAAGTATGGTACGGC+GGG 0.509532 1.1:+136351 MS.gene27109:CDS
AACAGGGAAGTAGATGGAGT+TGG 0.510251 1.1:-135551 None:intergenic
CGGCGGCACAAGTGGTAAGA+AGG 0.513016 1.1:-136121 None:intergenic
CATCGCTATGAGATTCTTGT+GGG 0.513470 1.1:-135905 None:intergenic
GCCTTCATTGACGCCTCCAC+CGG 0.515250 1.1:+135424 MS.gene27109:CDS
TGGAAGTGAAGGTGATGCTT+GGG 0.520153 1.1:-135713 None:intergenic
GGAGTAGTGAAAGCAAGAAC+AGG 0.520555 1.1:-135670 None:intergenic
GAAGATACTACTCCTTTGCT+TGG 0.520592 1.1:-135883 None:intergenic
ACTTCCTCACTCTCCAACAT+GGG 0.520727 1.1:+135493 MS.gene27109:CDS
TGCCTCTCCGTCATGTCCCT+CGG 0.521898 1.1:+135577 MS.gene27109:CDS
TTCCTTACCACATATGCCAT+TGG 0.522529 1.1:-138033 None:intergenic
AGCGATGGTTCAAGTTGTAA+TGG 0.525827 1.1:+135921 MS.gene27109:CDS
TCCGTTACTTCTTTACTCAA+AGG 0.528090 1.1:-136231 None:intergenic
GTTTGCTATATTGATAGCGA+TGG 0.538596 1.1:+137522 MS.gene27109:CDS
TTGGAAGTGAAGGTGATGCT+TGG 0.539417 1.1:-135714 None:intergenic
ATACAAAAGAATTCGAAAAG+TGG 0.543360 1.1:+138226 MS.gene27109:CDS
TTCCAATGGCATATGTGGTA+AGG 0.544112 1.1:+138031 MS.gene27109:CDS
CTTCATACGGTGGTTTCTGG+TGG 0.547015 1.1:+136204 MS.gene27109:CDS
ATCTGGTAAAATTCTTCGGA+AGG 0.547613 1.1:+138277 MS.gene27109:CDS
GGAGGCTGGGCAGGTTCCAA+TGG 0.547827 1.1:+138017 MS.gene27109:CDS
ACAGCAGCGTCTACAATAGC+AGG 0.550475 1.1:-137822 None:intergenic
GTAGGTGAATTGGTGGCCGG+TGG 0.552038 1.1:-135440 None:intergenic
ACCTTTACGGATCCCCATGT+TGG 0.552224 1.1:-135506 None:intergenic
GAAAGAGCTCATTAAGTACA+AGG 0.552506 1.1:+137569 MS.gene27109:CDS
GCATCTAAAGAGTGGTTTGG+TGG 0.553390 1.1:-135361 None:intergenic
CGTGGTCCCACCATTATGAA+AGG 0.554145 1.1:+136462 MS.gene27109:CDS
TCCAACATGGGGATCCGTAA+AGG 0.554642 1.1:+135505 MS.gene27109:CDS
GCAGGTTCCAATGGCATATG+TGG 0.556198 1.1:+138026 MS.gene27109:CDS
ACCAGTATCGGCGGCACAAG+TGG 0.558595 1.1:-136129 None:intergenic
AAACGAACCTTTCATAATGG+TGG 0.559397 1.1:-136469 None:intergenic
TTCAGCATAGCAACCAGTAT+CGG 0.561145 1.1:-136141 None:intergenic
CCAGTCCGATTAACTGGTAA+CGG 0.565679 1.1:-136426 None:intergenic
GGTATCCAAATAAGGAGGCT+GGG 0.569081 1.1:+138004 MS.gene27109:intron
CCATCGCTATGAGATTCTTG+TGG 0.569419 1.1:-135906 None:intergenic
GTGGCATCTAAAGAGTGGTT+TGG 0.570177 1.1:-135364 None:intergenic
CCGTTACCAGTTAATCGGAC+TGG 0.573347 1.1:+136426 MS.gene27109:CDS
TTGAGTAAAGAAGTAACGGA+AGG 0.574145 1.1:+136234 MS.gene27109:CDS
AGATGTATTGAGGGGATTGG+TGG 0.576476 1.1:-135614 None:intergenic
ATCTCCTCCCGCGCTCATCA+CGG 0.577531 1.1:+135394 MS.gene27109:CDS
TCCTTTGAGTAAAGAAGTAA+CGG 0.584099 1.1:+136230 MS.gene27109:CDS
GATTTCGCGAGATGTATTGA+GGG 0.584385 1.1:-135623 None:intergenic
AATTCACCTACCAACAACTC+TGG 0.585675 1.1:+135452 MS.gene27109:CDS
AACGAACCTTTCATAATGGT+GGG 0.587290 1.1:-136468 None:intergenic
GATATGGCCGTGATGAGCGC+GGG 0.587698 1.1:-135401 None:intergenic
AGATGGAGTTGGGAGAGAGG+AGG 0.592217 1.1:-135540 None:intergenic
TGAGTAAAGAAGTAACGGAA+GGG 0.592240 1.1:+136235 MS.gene27109:CDS
GTTGTTGGTAGGTGAATTGG+TGG 0.596032 1.1:-135447 None:intergenic
AATGGCATATGTGGTAAGGA+AGG 0.598196 1.1:+138035 MS.gene27109:CDS
GTCTTGCAGTGTTTGCTATG+GGG 0.598502 1.1:+136013 MS.gene27109:CDS
CGATATGGCCGTGATGAGCG+CGG 0.598855 1.1:-135402 None:intergenic
TGACGGAGAGGCAGACAACA+GGG 0.598859 1.1:-135567 None:intergenic
GTTGGAGAGTGAGGAAGTGA+CGG 0.598886 1.1:-135488 None:intergenic
CGACCATTACTCCAGAGGGT+TGG 0.603546 1.1:+137484 MS.gene27109:CDS
GATAGCGCCGAGGGACATGA+CGG 0.604148 1.1:-135584 None:intergenic
AGAGTGATCGATCCAAGAAG+TGG 0.604701 1.1:+135286 MS.gene27109:CDS
AGTGACTGAGCGCATCAACC+AGG 0.608319 1.1:+135819 MS.gene27109:CDS
TGTTGATGATGAGTTGCTGG+TGG 0.609037 1.1:-135752 None:intergenic
ATGGCCGTGATGAGCGCGGG+AGG 0.610511 1.1:-135398 None:intergenic
CCACAAGAATCTCATAGCGA+TGG 0.611191 1.1:+135906 MS.gene27109:CDS
GGTCCCTCCAGCAGAACTAG+AGG 0.614140 1.1:+137782 MS.gene27109:intron
TTTGAGTTCGCTTCATACGG+TGG 0.616174 1.1:+136194 MS.gene27109:CDS
GATCCCCATGTTGGAGAGTG+AGG 0.617341 1.1:-135497 None:intergenic
GGTGGTGATGATAGCGCCGA+GGG 0.619275 1.1:-135593 None:intergenic
AGAGAGTCGTAAGTATGGTA+CGG 0.623595 1.1:+136347 MS.gene27109:CDS
AGCGCGGGAGGAGATGAAAG+TGG 0.628660 1.1:-135386 None:intergenic
GGAACAACTGGACCAAGCAA+AGG 0.634924 1.1:+135871 MS.gene27109:CDS
TCAGTCACTCTACTCAACTC+AGG 0.638010 1.1:-135805 None:intergenic
GTAGTGTGGCTGTATCATCC+TGG 0.639019 1.1:-135837 None:intergenic
ATGAGAATGATTGGAAGTGA+AGG 0.639263 1.1:-135724 None:intergenic
AAAGCCTCTAGTTCTGCTGG+AGG 0.643862 1.1:-137786 None:intergenic
AGCATAGCAACCAGTATCGG+CGG 0.644393 1.1:-136138 None:intergenic
GGCAGGTTATTTCAGTAACG+AGG 0.647450 1.1:+137449 MS.gene27109:intron
ACATCGACCATTACTCCAGA+GGG 0.647619 1.1:+137480 MS.gene27109:CDS
AAAGAGCTCATTAAGTACAA+GGG 0.651964 1.1:+137570 MS.gene27109:CDS
GAGTGATCGATCCAAGAAGT+GGG 0.652473 1.1:+135287 MS.gene27109:CDS
AGACCGTATATATGAAACAT+CGG 0.653796 1.1:-135994 None:intergenic
GCTTGTGTCTGCTTCCACGG+AGG 0.655096 1.1:+136374 MS.gene27109:CDS
CTCTGGAGTAATGGTCGATG+TGG 0.656546 1.1:-137478 None:intergenic
AAGCCTCTAGTTCTGCTGGA+GGG 0.656701 1.1:-137785 None:intergenic
ATTTCGCGAGATGTATTGAG+GGG 0.663874 1.1:-135622 None:intergenic
CTTCCTCACTCTCCAACATG+GGG 0.667378 1.1:+135494 MS.gene27109:CDS
GCGGGAGGAGATGAAAGTGG+TGG 0.667975 1.1:-135383 None:intergenic
AGGTTATTTCAGTAACGAGG+AGG 0.670840 1.1:+137452 MS.gene27109:intron
CGCCGAGGGACATGACGGAG+AGG 0.671164 1.1:-135579 None:intergenic
AAGTGGTGGCATCTAAAGAG+TGG 0.673146 1.1:-135369 None:intergenic
TATCAGGTATCCAAATAAGG+AGG 0.676684 1.1:+137999 MS.gene27109:intron
CACATCGACCATTACTCCAG+AGG 0.683319 1.1:+137479 MS.gene27109:CDS
TCCTGATGAGTAGAGTAGTG+TGG 0.691172 1.1:-135851 None:intergenic
GTCGTAAGTATGGTACGGCG+GGG 0.691371 1.1:+136352 MS.gene27109:CDS
AGCACGCCAGAGTTGTTGGT+AGG 0.696936 1.1:-135458 None:intergenic
AGTAGAAGCTCCAATTCCAG+AGG 0.697004 1.1:-136313 None:intergenic
TGGTGGTGATGATAGCGCCG+AGG 0.700534 1.1:-135594 None:intergenic
GTCAACAATCATAGCCTCCG+TGG 0.700669 1.1:-136388 None:intergenic
GAGTCGTAAGTATGGTACGG+CGG 0.704214 1.1:+136350 MS.gene27109:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTAATTAATTAAAAA+AGG - chr1.1:136630-136649 None:intergenic 0.0%
!! ATTAATTTATATTCTTTGGT+AGG - chr1.1:137859-137878 None:intergenic 15.0%
!! GAAAAAAATTAAAGTTGTAT+TGG - chr1.1:136832-136851 None:intergenic 15.0%
!!! AATTTTTTTCAATATATAGG+TGG + chr1.1:138199-138218 MS.gene27109:intron 15.0%
!! AAATGGAATGAAATAATCAT+TGG - chr1.1:136957-136976 None:intergenic 20.0%
!! AGTAAAATGTCAAAATTGTT+AGG - chr1.1:137162-137181 None:intergenic 20.0%
!! ATAAACAATTCAGATTAGAT+TGG + chr1.1:136557-136576 MS.gene27109:intron 20.0%
!! TAATTAAAATATTGAACACG+AGG + chr1.1:136638-136657 MS.gene27109:intron 20.0%
!!! ATCGATATTCTAAAAAATGT+GGG - chr1.1:137067-137086 None:intergenic 20.0%
!!! GTTACTTTTTTTATGTCTAA+TGG - chr1.1:137261-137280 None:intergenic 20.0%
!!! TATCGATATTCTAAAAAATG+TGG - chr1.1:137068-137087 None:intergenic 20.0%
!!! TGTCTTTTTCTAATAAAGTA+AGG + chr1.1:136908-136927 MS.gene27109:intron 20.0%
!!! TTTTGTGTGTATATTATATC+AGG + chr1.1:137983-138002 MS.gene27109:intron 20.0%
! AATTATAGCATATAAACTCG+TGG - chr1.1:136606-136625 None:intergenic 25.0%
! ATACAAAAGAATTCGAAAAG+TGG + chr1.1:138226-138245 MS.gene27109:CDS 25.0%
! ATATAAGCTCACATAATTGT+TGG + chr1.1:137382-137401 MS.gene27109:intron 25.0%
! CACTAAAATAATAGATGCAA+AGG + chr1.1:137320-137339 MS.gene27109:intron 25.0%
! GTAAACAGTAATTTATCTCT+GGG - chr1.1:137674-137693 None:intergenic 25.0%
! TCTTTATTACTATTGTGTGT+CGG - chr1.1:137696-137715 None:intergenic 25.0%
! TTATATCAGGTATCCAAATA+AGG + chr1.1:137996-138015 MS.gene27109:intron 25.0%
!! ACTTTTCGAATTCTTTTGTA+TGG - chr1.1:138227-138246 None:intergenic 25.0%
!! TGTTTTATGGTTATTGTTAC+AGG + chr1.1:137761-137780 MS.gene27109:intron 25.0%
!! TTTTAGTGTTATAAACACCT+TGG - chr1.1:137308-137327 None:intergenic 25.0%
!!! AATTTTACCAGATGGATTTT+TGG - chr1.1:138270-138289 None:intergenic 25.0%
!!! ACTCTTTTTTATTAGATGCT+TGG + chr1.1:138141-138160 MS.gene27109:intron 25.0%
!!! ATTTTACCAGATGGATTTTT+GGG - chr1.1:138269-138288 None:intergenic 25.0%
!!! CATAATTGTTGGTTCTATTA+TGG + chr1.1:137393-137412 MS.gene27109:intron 25.0%
!!! CTCTTTTTTATTAGATGCTT+GGG + chr1.1:138142-138161 MS.gene27109:intron 25.0%
AAACTCACACAAATAGATAC+TGG + chr1.1:137113-137132 MS.gene27109:intron 30.0%
AAAGAGCTCATTAAGTACAA+GGG + chr1.1:137570-137589 MS.gene27109:CDS 30.0%
AACTCACACAAATAGATACT+GGG + chr1.1:137114-137133 MS.gene27109:intron 30.0%
AAGAAACGAACCTTTCATAA+TGG - chr1.1:136475-136494 None:intergenic 30.0%
AGACCGTATATATGAAACAT+CGG - chr1.1:135997-136016 None:intergenic 30.0%
ATCCATCTGGTAAAATTCTT+CGG + chr1.1:138273-138292 MS.gene27109:CDS 30.0%
ATTAAAATGCAAGACATGGT+TGG + chr1.1:137422-137441 MS.gene27109:intron 30.0%
CCTAATGTAAGTATCTTTCA+AGG + chr1.1:136273-136292 MS.gene27109:CDS 30.0%
CCTTGAAAGATACTTACATT+AGG - chr1.1:136276-136295 None:intergenic 30.0%
CGTAAATATTGTCAAACTCA+TGG + chr1.1:137723-137742 MS.gene27109:intron 30.0%
GAATTTAGAGAGAACAACTA+TGG - chr1.1:136058-136077 None:intergenic 30.0%
GCTAATTAAAATGCAAGACA+TGG + chr1.1:137418-137437 MS.gene27109:intron 30.0%
GGTAAACAGTAATTTATCTC+TGG - chr1.1:137675-137694 None:intergenic 30.0%
GTAAGTATCTTTCAAGGTTA+TGG + chr1.1:136279-136298 MS.gene27109:CDS 30.0%
GTTCCGATGTTTCATATATA+CGG + chr1.1:135991-136010 MS.gene27109:CDS 30.0%
TATACACACAAAACATTCAG+CGG - chr1.1:137976-137995 None:intergenic 30.0%
TATATTCTTTGGTAGGCTTA+CGG - chr1.1:137852-137871 None:intergenic 30.0%
TCCTTTGAGTAAAGAAGTAA+CGG + chr1.1:136230-136249 MS.gene27109:CDS 30.0%
TGAATTGCTAAACAAGTTTC+CGG - chr1.1:136669-136688 None:intergenic 30.0%
TGATTGATAAGAAGGAATAC+TGG - chr1.1:137950-137969 None:intergenic 30.0%
!! AAGTGACACTCATTTTGAAA+CGG - chr1.1:136766-136785 None:intergenic 30.0%
!!! GTTCTCTTGCTTTTGTTTTA+TGG + chr1.1:137748-137767 MS.gene27109:intron 30.0%
AAACGAACCTTTCATAATGG+TGG - chr1.1:136472-136491 None:intergenic 35.0%
AAAGTTTGAGAGGGTAAAGT+TGG - chr1.1:136857-136876 None:intergenic 35.0%
AACGAACCTTTCATAATGGT+GGG - chr1.1:136471-136490 None:intergenic 35.0%
AATGAGAACATGTGGTCTAT+TGG + chr1.1:136725-136744 MS.gene27109:intron 35.0%
ACTCACACAAATAGATACTG+GGG + chr1.1:137115-137134 MS.gene27109:intron 35.0%
ATCTGGTAAAATTCTTCGGA+AGG + chr1.1:138277-138296 MS.gene27109:CDS 35.0%
ATGAGAATGATTGGAAGTGA+AGG - chr1.1:135727-135746 None:intergenic 35.0%
CAAGTTGTAATGGCTAGATT+TGG + chr1.1:135931-135950 MS.gene27109:CDS 35.0%
CATTAAGTACAAGGGATATC+AGG + chr1.1:137578-137597 MS.gene27109:CDS 35.0%
CTGCAACAAAATCCATAACT+TGG - chr1.1:138083-138102 None:intergenic 35.0%
GAAAGAGCTCATTAAGTACA+AGG + chr1.1:137569-137588 MS.gene27109:CDS 35.0%
GAATTGCTAAACAAGTTTCC+GGG - chr1.1:136668-136687 None:intergenic 35.0%
TAATACCAGCAACTAACCAA+AGG - chr1.1:137361-137380 None:intergenic 35.0%
TAGATGCAAAGGAAAAAGCT+TGG + chr1.1:137331-137350 MS.gene27109:intron 35.0%
TATCAGGTATCCAAATAAGG+AGG + chr1.1:137999-138018 MS.gene27109:intron 35.0%
TATGGTTTAACTGAATCCTC+TGG + chr1.1:136297-136316 MS.gene27109:CDS 35.0%
TCCGTTACTTCTTTACTCAA+AGG - chr1.1:136234-136253 None:intergenic 35.0%
TCTATACCCAAAAATCCATC+TGG + chr1.1:138260-138279 MS.gene27109:CDS 35.0%
TGTGTGAGAAAAGTTTGAGA+GGG - chr1.1:136866-136885 None:intergenic 35.0%
TTCACTTCCAATCATTCTCA+TGG + chr1.1:135726-135745 MS.gene27109:CDS 35.0%
TTGATGAGAATGAGAACATG+TGG + chr1.1:136717-136736 MS.gene27109:intron 35.0%
TTGGCACACAGATTAAGAAA+TGG - chr1.1:136974-136993 None:intergenic 35.0%
TTGTGTGAGAAAAGTTTGAG+AGG - chr1.1:136867-136886 None:intergenic 35.0%
! TGAGTAAAGAAGTAACGGAA+GGG + chr1.1:136235-136254 MS.gene27109:CDS 35.0%
! TGATTTGAGTTCGCTTCATA+CGG + chr1.1:136191-136210 MS.gene27109:CDS 35.0%
! TTCCGAAGAATTTTACCAGA+TGG - chr1.1:138278-138297 None:intergenic 35.0%
! TTGAGTAAAGAAGTAACGGA+AGG + chr1.1:136234-136253 MS.gene27109:CDS 35.0%
!! ATGCTTGGGTGATTTTAGAA+AGG - chr1.1:135702-135721 None:intergenic 35.0%
!! GTGATTTTAGAAAGGAGTTG+TGG - chr1.1:135694-135713 None:intergenic 35.0%
!! GTTTGCTATATTGATAGCGA+TGG + chr1.1:137522-137541 MS.gene27109:CDS 35.0%
!! TTAACTGAATCCTCTGGAAT+TGG + chr1.1:136303-136322 MS.gene27109:CDS 35.0%
!! TTCAAAATGAGTGTCGCTTT+AGG - chr1.1:136999-137018 None:intergenic 35.0%
!!! GACACTCATTTTGAAACAGA+GGG + chr1.1:137004-137023 MS.gene27109:intron 35.0%
AATGGCATATGTGGTAAGGA+AGG + chr1.1:138035-138054 MS.gene27109:CDS 40.0%
AATTCACCTACCAACAACTC+TGG + chr1.1:135452-135471 MS.gene27109:CDS 40.0%
AGAGAGTCGTAAGTATGGTA+CGG + chr1.1:136347-136366 MS.gene27109:CDS 40.0%
AGCGGTCTTGATTGATAAGA+AGG - chr1.1:137958-137977 None:intergenic 40.0%
AGTCATGCACGTCTAATGAA+TGG - chr1.1:137283-137302 None:intergenic 40.0%
AGTGAAAGCAAGAACAGGTT+TGG - chr1.1:135668-135687 None:intergenic 40.0%
ATTTCGCGAGATGTATTGAG+GGG - chr1.1:135625-135644 None:intergenic 40.0%
CATCGCTATGAGATTCTTGT+GGG - chr1.1:135908-135927 None:intergenic 40.0%
GAAGATACTACTCCTTTGCT+TGG - chr1.1:135886-135905 None:intergenic 40.0%
GATTTCGCGAGATGTATTGA+GGG - chr1.1:135626-135645 None:intergenic 40.0%
GGAAAGACAAAACATCCAGA+TGG + chr1.1:136578-136597 MS.gene27109:intron 40.0%
GTTGGTCATATGCTTGACTT+AGG + chr1.1:137033-137052 MS.gene27109:intron 40.0%
TAGTTGAAGTGATCGAAACG+TGG - chr1.1:137646-137665 None:intergenic 40.0%
TATAAACTCGTGGATCCATC+TGG - chr1.1:136596-136615 None:intergenic 40.0%
TGAAACAGAGGGAGTAATGT+TGG + chr1.1:137015-137034 MS.gene27109:intron 40.0%
TTCAGCATAGCAACCAGTAT+CGG - chr1.1:136144-136163 None:intergenic 40.0%
TTCCAATGGCATATGTGGTA+AGG + chr1.1:138031-138050 MS.gene27109:CDS 40.0%
TTCCTTACCACATATGCCAT+TGG - chr1.1:138036-138055 None:intergenic 40.0%
TTGGAAGAGAGTCGTAAGTA+TGG + chr1.1:136342-136361 MS.gene27109:CDS 40.0%
! ACTGTTTGAGTTCGAACTTG+TGG + chr1.1:137179-137198 MS.gene27109:intron 40.0%
! AGAGTTGTTGGTAGGTGAAT+TGG - chr1.1:135453-135472 None:intergenic 40.0%
! AGGTTATTTCAGTAACGAGG+AGG + chr1.1:137452-137471 MS.gene27109:intron 40.0%
! CGATGGATTCTTATTTGTCG+TGG + chr1.1:137539-137558 MS.gene27109:CDS 40.0%
! CTGTTTGAGTTCGAACTTGT+GGG + chr1.1:137180-137199 MS.gene27109:intron 40.0%
! TATGGATTTTGTTGCAGAGC+AGG + chr1.1:138086-138105 MS.gene27109:CDS 40.0%
!! AGAGGGTTGGTTAAAAACAG+GGG + chr1.1:137497-137516 MS.gene27109:CDS 40.0%
!! AGCGATGGTTCAAGTTGTAA+TGG + chr1.1:135921-135940 MS.gene27109:CDS 40.0%
!! CAGAGGGTTGGTTAAAAACA+GGG + chr1.1:137496-137515 MS.gene27109:CDS 40.0%
!! TGTTGTTGATGATGAGTTGC+TGG - chr1.1:135758-135777 None:intergenic 40.0%
!!! CGACACTCATTTTGAAACAG+AGG + chr1.1:137003-137022 MS.gene27109:intron 40.0%
!!! GACACTCATTTTGAAACGGA+TGG - chr1.1:136762-136781 None:intergenic 40.0%
AACAGGGAAGTAGATGGAGT+TGG - chr1.1:135554-135573 None:intergenic 45.0%
AAGTGGTGGCATCTAAAGAG+TGG - chr1.1:135372-135391 None:intergenic 45.0%
ACAGGGAAGTAGATGGAGTT+GGG - chr1.1:135553-135572 None:intergenic 45.0%
ACATCGACCATTACTCCAGA+GGG + chr1.1:137480-137499 MS.gene27109:CDS 45.0%
ACTTCCTCACTCTCCAACAT+GGG + chr1.1:135493-135512 MS.gene27109:CDS 45.0%
AGAGTGATCGATCCAAGAAG+TGG + chr1.1:135286-135305 MS.gene27109:CDS 45.0%
AGATGTATTGAGGGGATTGG+TGG - chr1.1:135617-135636 None:intergenic 45.0%
AGGTATCCAAATAAGGAGGC+TGG + chr1.1:138003-138022 MS.gene27109:intron 45.0%
AGTAGAAGCTCCAATTCCAG+AGG - chr1.1:136316-136335 None:intergenic 45.0%
CCACAAGAATCTCATAGCGA+TGG + chr1.1:135906-135925 MS.gene27109:CDS 45.0%
CCAGTCCGATTAACTGGTAA+CGG - chr1.1:136429-136448 None:intergenic 45.0%
CCATCGCTATGAGATTCTTG+TGG - chr1.1:135909-135928 None:intergenic 45.0%
CGATTTCGCGAGATGTATTG+AGG - chr1.1:135627-135646 None:intergenic 45.0%
CTCAGCCGTTACCAGTTAAT+CGG + chr1.1:136421-136440 MS.gene27109:CDS 45.0%
CTCTACTCATCAGGAACAAC+TGG + chr1.1:135859-135878 MS.gene27109:CDS 45.0%
CTTGTCAGGAAGCCAAGTTA+TGG + chr1.1:138068-138087 MS.gene27109:CDS 45.0%
GAGTGATCGATCCAAGAAGT+GGG + chr1.1:135287-135306 MS.gene27109:CDS 45.0%
GGAGTAGTGAAAGCAAGAAC+AGG - chr1.1:135673-135692 None:intergenic 45.0%
GGTATCCAAATAAGGAGGCT+GGG + chr1.1:138004-138023 MS.gene27109:intron 45.0%
GTGGCATCTAAAGAGTGGTT+TGG - chr1.1:135367-135386 None:intergenic 45.0%
GTGGTGTCCATGAGAATGAT+TGG - chr1.1:135736-135755 None:intergenic 45.0%
TATTGAACACGAGGTAGACC+CGG + chr1.1:136647-136666 MS.gene27109:intron 45.0%
TCAGTCACTCTACTCAACTC+AGG - chr1.1:135808-135827 None:intergenic 45.0%
TCCTGATGAGTAGAGTAGTG+TGG - chr1.1:135854-135873 None:intergenic 45.0%
TGGAAGTGAAGGTGATGCTT+GGG - chr1.1:135716-135735 None:intergenic 45.0%
TGGAGCTTCTACTGAGTCTT+TGG + chr1.1:136323-136342 MS.gene27109:CDS 45.0%
TTGGAAGTGAAGGTGATGCT+TGG - chr1.1:135717-135736 None:intergenic 45.0%
! GAAAAAGCTTGGTGAGCCTT+TGG + chr1.1:137342-137361 MS.gene27109:intron 45.0%
! GCAGAGCAGGTTTGTTTGTT+TGG + chr1.1:138099-138118 MS.gene27109:intron 45.0%
! GCATCTAAAGAGTGGTTTGG+TGG - chr1.1:135364-135383 None:intergenic 45.0%
! GGCAGGTTATTTCAGTAACG+AGG + chr1.1:137449-137468 MS.gene27109:intron 45.0%
! GTTGTTGGTAGGTGAATTGG+TGG - chr1.1:135450-135469 None:intergenic 45.0%
! TTTGAGTTCGCTTCATACGG+TGG + chr1.1:136194-136213 MS.gene27109:CDS 45.0%
!! AAAGAGTGGTTTGGTGGAAG+TGG - chr1.1:135358-135377 None:intergenic 45.0%
!! AACAAAGCCTCTAGTTCTGC+TGG - chr1.1:137792-137811 None:intergenic 45.0%
!! ATGAGTGTCGCTTTAGGCTT+TGG - chr1.1:136993-137012 None:intergenic 45.0%
!! ATGCAAGACATGGTTGGTGA+TGG + chr1.1:137428-137447 MS.gene27109:intron 45.0%
!! CCAGAGGGTTGGTTAAAAAC+AGG + chr1.1:137495-137514 MS.gene27109:CDS 45.0%
!! GGTCTTGCAGTGTTTGCTAT+GGG + chr1.1:136012-136031 MS.gene27109:CDS 45.0%
!! GTCTTGCAGTGTTTGCTATG+GGG + chr1.1:136013-136032 MS.gene27109:CDS 45.0%
!! TAACCAACCCTCTGGAGTAA+TGG - chr1.1:137490-137509 None:intergenic 45.0%
!! TGTTGATGATGAGTTGCTGG+TGG - chr1.1:135755-135774 None:intergenic 45.0%
!! TTAATCGGACTGGTGAGCTT+TGG + chr1.1:136436-136455 MS.gene27109:CDS 45.0%
!!! CCTGTTTTTAACCAACCCTC+TGG - chr1.1:137498-137517 None:intergenic 45.0%
!! TATAATTAATTTATATTCTT+TGG - chr1.1:137863-137882 None:intergenic 5.0%
!!! ATTAATTTTTTTCAATATAT+AGG + chr1.1:138196-138215 MS.gene27109:intron 5.0%
!!! ATTTATTTTTTATAATATGA+TGG + chr1.1:137915-137934 MS.gene27109:intron 5.0%
ACAGCAGCGTCTACAATAGC+AGG - chr1.1:137825-137844 None:intergenic 50.0%
ACCTTTACGGATCCCCATGT+TGG - chr1.1:135509-135528 None:intergenic 50.0%
AGATACTGGGGATCGAATCC+TGG + chr1.1:137127-137146 MS.gene27109:intron 50.0%
AGCTCACCAGTCCGATTAAC+TGG - chr1.1:136435-136454 None:intergenic 50.0%
AGGCGTCAATGAAGGCGATA+TGG - chr1.1:135420-135439 None:intergenic 50.0%
AGTAGATGGAGTTGGGAGAG+AGG - chr1.1:135546-135565 None:intergenic 50.0%
AGTCGTAAGTATGGTACGGC+GGG + chr1.1:136351-136370 MS.gene27109:CDS 50.0%
ATTGTTAGGCAGAGCACACC+AGG - chr1.1:137148-137167 None:intergenic 50.0%
CACATCGACCATTACTCCAG+AGG + chr1.1:137479-137498 MS.gene27109:CDS 50.0%
CACTTCCTCACTCTCCAACA+TGG + chr1.1:135492-135511 MS.gene27109:CDS 50.0%
CCGTTACCAGTTAATCGGAC+TGG + chr1.1:136426-136445 MS.gene27109:CDS 50.0%
CGTGGTCCCACCATTATGAA+AGG + chr1.1:136462-136481 MS.gene27109:CDS 50.0%
CTGCTGCAAAACCCACTTCT+TGG - chr1.1:135301-135320 None:intergenic 50.0%
CTTCCTCACTCTCCAACATG+GGG + chr1.1:135494-135513 MS.gene27109:CDS 50.0%
GAGTCGTAAGTATGGTACGG+CGG + chr1.1:136350-136369 MS.gene27109:CDS 50.0%
GCAGACAACAGGGAAGTAGA+TGG - chr1.1:135560-135579 None:intergenic 50.0%
GCAGGTTCCAATGGCATATG+TGG + chr1.1:138026-138045 MS.gene27109:CDS 50.0%
GCATATGTGGTAAGGAAGGC+TGG + chr1.1:138039-138058 MS.gene27109:CDS 50.0%
GCCACACTACTCTACTCATC+AGG + chr1.1:135850-135869 MS.gene27109:CDS 50.0%
GCGAGATGTATTGAGGGGAT+TGG - chr1.1:135620-135639 None:intergenic 50.0%
GGAACAACTGGACCAAGCAA+AGG + chr1.1:135871-135890 MS.gene27109:CDS 50.0%
GGAGGATGACGTCACCTTTA+CGG - chr1.1:135522-135541 None:intergenic 50.0%
GTAGTGTGGCTGTATCATCC+TGG - chr1.1:135840-135859 None:intergenic 50.0%
GTCAACAATCATAGCCTCCG+TGG - chr1.1:136391-136410 None:intergenic 50.0%
GTTGGAGAGTGAGGAAGTGA+CGG - chr1.1:135491-135510 None:intergenic 50.0%
TAGACGTGCATGACTCTCCA+AGG + chr1.1:137288-137307 MS.gene27109:intron 50.0%
TCCAACATGGGGATCCGTAA+AGG + chr1.1:135505-135524 MS.gene27109:CDS 50.0%
TCGCTTCATACGGTGGTTTC+TGG + chr1.1:136201-136220 MS.gene27109:CDS 50.0%
TGCTATGGGGCTTCTTGCTA+TGG + chr1.1:136026-136045 MS.gene27109:CDS 50.0%
! AAGGCTGGAAGTAGCTTGTC+AGG + chr1.1:138054-138073 MS.gene27109:CDS 50.0%
! AGCATAGCAACCAGTATCGG+CGG - chr1.1:136141-136160 None:intergenic 50.0%
! CGGTCTTGCAGTGTTTGCTA+TGG + chr1.1:136011-136030 MS.gene27109:CDS 50.0%
! CTTCATACGGTGGTTTCTGG+TGG + chr1.1:136204-136223 MS.gene27109:CDS 50.0%
! GCTATGGGGCTTCTTGCTAT+GGG + chr1.1:136027-136046 MS.gene27109:CDS 50.0%
! GTGAGCCTTTGGTTAGTTGC+TGG + chr1.1:137353-137372 MS.gene27109:intron 50.0%
! TGTATTGAGGGGATTGGTGG+TGG - chr1.1:135614-135633 None:intergenic 50.0%
! TTGGTAGGTGAATTGGTGGC+CGG - chr1.1:135446-135465 None:intergenic 50.0%
!! AAAGCCTCTAGTTCTGCTGG+AGG - chr1.1:137789-137808 None:intergenic 50.0%
!! AAGACATGGTTGGTGATGGC+AGG + chr1.1:137432-137451 MS.gene27109:intron 50.0%
!! AAGCCTCTAGTTCTGCTGGA+GGG - chr1.1:137788-137807 None:intergenic 50.0%
!! AGTGGTTTGGTGGAAGTGGA+AGG - chr1.1:135354-135373 None:intergenic 50.0%
!! CTCTGGAGTAATGGTCGATG+TGG - chr1.1:137481-137500 None:intergenic 50.0%
!! GTGGTTTGGTGGAAGTGGAA+GGG - chr1.1:135353-135372 None:intergenic 50.0%
!! TGGTTTGGTGGAAGTGGAAG+GGG - chr1.1:135352-135371 None:intergenic 50.0%
ACCTGCCCAGCCTCCTTATT+TGG - chr1.1:138012-138031 None:intergenic 55.0%
AGATGGAGTTGGGAGAGAGG+AGG - chr1.1:135543-135562 None:intergenic 55.0%
AGCACGCCAGAGTTGTTGGT+AGG - chr1.1:135461-135480 None:intergenic 55.0%
AGTGACTGAGCGCATCAACC+AGG + chr1.1:135819-135838 MS.gene27109:CDS 55.0%
CGACCATTACTCCAGAGGGT+TGG + chr1.1:137484-137503 MS.gene27109:CDS 55.0%
CTACAGCACGCCAGAGTTGT+TGG - chr1.1:135465-135484 None:intergenic 55.0%
GATCCCCATGTTGGAGAGTG+AGG - chr1.1:135500-135519 None:intergenic 55.0%
GTCGTAAGTATGGTACGGCG+GGG + chr1.1:136352-136371 MS.gene27109:CDS 55.0%
TCCAAATAAGGAGGCTGGGC+AGG + chr1.1:138008-138027 MS.gene27109:CDS 55.0%
TGACGGAGAGGCAGACAACA+GGG - chr1.1:135570-135589 None:intergenic 55.0%
! ACTGGTGAGCTTTGGCTTCG+TGG + chr1.1:136444-136463 MS.gene27109:CDS 55.0%
! ATGACGGAGAGGCAGACAAC+AGG - chr1.1:135571-135590 None:intergenic 55.0%
ACCACTTGTGCCGCCGATAC+TGG + chr1.1:136128-136147 MS.gene27109:CDS 60.0%
AGCGCGGGAGGAGATGAAAG+TGG - chr1.1:135389-135408 None:intergenic 60.0%
ATCTCCTCCCGCGCTCATCA+CGG + chr1.1:135394-135413 MS.gene27109:CDS 60.0%
CGATATGGCCGTGATGAGCG+CGG - chr1.1:135405-135424 None:intergenic 60.0%
CGGCGGCACAAGTGGTAAGA+AGG - chr1.1:136124-136143 None:intergenic 60.0%
GATATGGCCGTGATGAGCGC+GGG - chr1.1:135404-135423 None:intergenic 60.0%
GCCTTCATTGACGCCTCCAC+CGG + chr1.1:135424-135443 MS.gene27109:CDS 60.0%
GCGGGAGGAGATGAAAGTGG+TGG - chr1.1:135386-135405 None:intergenic 60.0%
GCTTGTGTCTGCTTCCACGG+AGG + chr1.1:136374-136393 MS.gene27109:CDS 60.0%
GGGGCTTGTGTCTGCTTCCA+CGG + chr1.1:136371-136390 MS.gene27109:CDS 60.0%
GGTCCCTCCAGCAGAACTAG+AGG + chr1.1:137782-137801 MS.gene27109:intron 60.0%
TGCCTCTCCGTCATGTCCCT+CGG + chr1.1:135577-135596 MS.gene27109:CDS 60.0%
TGGAGTTGGGAGAGAGGAGG+AGG - chr1.1:135540-135559 None:intergenic 60.0%
! GATAGCGCCGAGGGACATGA+CGG - chr1.1:135587-135606 None:intergenic 60.0%
! GGTGGTGATGATAGCGCCGA+GGG - chr1.1:135596-135615 None:intergenic 60.0%
! GTAGGTGAATTGGTGGCCGG+TGG - chr1.1:135443-135462 None:intergenic 60.0%
! TGGTGGTGATGATAGCGCCG+AGG - chr1.1:135597-135616 None:intergenic 60.0%
!! ACCAGTATCGGCGGCACAAG+TGG - chr1.1:136132-136151 None:intergenic 60.0%
GGAGGCTGGGCAGGTTCCAA+TGG + chr1.1:138017-138036 MS.gene27109:CDS 65.0%
! GGTGAATTGGTGGCCGGTGG+AGG - chr1.1:135440-135459 None:intergenic 65.0%
!! GCCGGTGGAGGCGTCAATGA+AGG - chr1.1:135428-135447 None:intergenic 65.0%
ATGGCCGTGATGAGCGCGGG+AGG - chr1.1:135401-135420 None:intergenic 70.0%
! CGCCGAGGGACATGACGGAG+AGG - chr1.1:135582-135601 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 135253 138331 135253 ID=MS.gene27109
chr1.1 mRNA 135253 138331 135253 ID=MS.gene27109.t1;Parent=MS.gene27109
chr1.1 exon 135253 136483 135253 ID=MS.gene27109.t1.exon1;Parent=MS.gene27109.t1
chr1.1 CDS 135253 136483 135253 ID=cds.MS.gene27109.t1;Parent=MS.gene27109.t1
chr1.1 exon 137454 137599 137454 ID=MS.gene27109.t1.exon2;Parent=MS.gene27109.t1
chr1.1 CDS 137454 137599 137454 ID=cds.MS.gene27109.t1;Parent=MS.gene27109.t1
chr1.1 exon 137783 137850 137783 ID=MS.gene27109.t1.exon3;Parent=MS.gene27109.t1
chr1.1 CDS 137783 137850 137783 ID=cds.MS.gene27109.t1;Parent=MS.gene27109.t1
chr1.1 exon 138005 138107 138005 ID=MS.gene27109.t1.exon4;Parent=MS.gene27109.t1
chr1.1 CDS 138005 138107 138005 ID=cds.MS.gene27109.t1;Parent=MS.gene27109.t1
chr1.1 exon 138218 138331 138218 ID=MS.gene27109.t1.exon5;Parent=MS.gene27109.t1
chr1.1 CDS 138218 138331 138218 ID=cds.MS.gene27109.t1;Parent=MS.gene27109.t1
Gene Sequence

>MS.gene27109

ATGGAGGTAAACGGTGAGAATACGAATAGCAGAAGAGTGATCGATCCAAGAAGTGGGTTTTGCAGCAGCAATTCAATCTTCTACAGCAAGAGAAAACCCCTTCCACTTCCACCAAACCACTCTTTAGATGCCACCACTTTCATCTCCTCCCGCGCTCATCACGGCCATATCGCCTTCATTGACGCCTCCACCGGCCACCAATTCACCTACCAACAACTCTGGCGTGCTGTAGACTCCGTCACTTCCTCACTCTCCAACATGGGGATCCGTAAAGGTGACGTCATCCTCCTCCTCTCTCCCAACTCCATCTACTTCCCTGTTGTCTGCCTCTCCGTCATGTCCCTCGGCGCTATCATCACCACCACCAATCCCCTCAATACATCTCGCGAAATCGCTAAACAGATCGCTGATTCCAAACCTGTTCTTGCTTTCACTACTCCACAACTCCTTTCTAAAATCACCCAAGCATCACCTTCACTTCCAATCATTCTCATGGACACCACCAGCAACTCATCATCAACAACAACACTTGATGAAATGATGAAGAAAGAACCTGAGTTGAGTAGAGTGACTGAGCGCATCAACCAGGATGATACAGCCACACTACTCTACTCATCAGGAACAACTGGACCAAGCAAAGGAGTAGTATCTTCCCACAAGAATCTCATAGCGATGGTTCAAGTTGTAATGGCTAGATTTGGTAAAGAACAAGAGACGAGAGAAACTTTCATATGCACTGTTCCGATGTTTCATATATACGGTCTTGCAGTGTTTGCTATGGGGCTTCTTGCTATGGGTTCAACCATAGTTGTTCTCTCTAAATTCGAGATGCATGACATGCTTTCATCTATTGAGAAATTCAGAGCAACCTTCTTACCACTTGTGCCGCCGATACTGGTTGCTATGCTGAACAACGCAGATGCGATTAAGAGAAAGTATGATTTGAGTTCGCTTCATACGGTGGTTTCTGGTGGTGCTCCTTTGAGTAAAGAAGTAACGGAAGGGTTTATAGAAAAGTATCCTAATGTAAGTATCTTTCAAGGTTATGGTTTAACTGAATCCTCTGGAATTGGAGCTTCTACTGAGTCTTTGGAAGAGAGTCGTAAGTATGGTACGGCGGGGCTTGTGTCTGCTTCCACGGAGGCTATGATTGTTGACACTGAGACTGCTCAGCCGTTACCAGTTAATCGGACTGGTGAGCTTTGGCTTCGTGGTCCCACCATTATGAAAGGTTCGTTTCTTTTCAAATGTCGTCGCTTTGTTTCTAATTAATTGATCTTTTGATTCTGAAGATCGCTCTTTTAATAAACAATTCAGATTAGATTGGAAAGACAAAACATCCAGATGGATCCACGAGTTTATATGCTATAATTTCCTTTTTTAATTAATTAAAATATTGAACACGAGGTAGACCCGGAAACTTGTTTAGCAATTCAGAGTTTATATATTTGTATTTGTAGTTGATTGATGAGAATGAGAACATGTGGTCTATTGGAATTTAATACTCCATCCGTTTCAAAATGAGTGTCACTTTAAGCAAAAAAATTTGTTTCAAAATGAATGTCGCTTTACATTTCCAATACAACTTTAATTTTTTTCTTCCAACTTTACCCTCTCAAACTTTTCTCACACAATTTTCAATGCAACTTTAAGTTTGTCTTTTTCTAATAAAGTAAGGACAGTTTAGTAAAAGCGTTATATCCAATGATTATTTCATTCCATTTCTTAATCTGTGTGCCAAAGCCTAAAGCGACACTCATTTTGAAACAGAGGGAGTAATGTTGGTCATATGCTTGACTTAGGCTATATATCCCACATTTTTTAGAATATCGATAGACAAAATGACAAAACATTTATTACAAACTCACACAAATAGATACTGGGGATCGAATCCTGGTGTGCTCTGCCTAACAATTTTGACATTTTACTGTTTGAGTTCGAACTTGTGGGCTTTCGCTTTTCTAATTTAATAAATTTAATTTTTATTTAATAAAAAAAGAAGTAACCATTAGACATAAAAAAAGTAACCATTCATTAGACGTGCATGACTCTCCAAGGTGTTTATAACACTAAAATAATAGATGCAAAGGAAAAAGCTTGGTGAGCCTTTGGTTAGTTGCTGGTATTAGATATAAGCTCACATAATTGTTGGTTCTATTATGGATGCTAATTAAAATGCAAGACATGGTTGGTGATGGCAGGTTATTTCAGTAACGAGGAGGCAACCACATCGACCATTACTCCAGAGGGTTGGTTAAAAACAGGGGATGTTTGCTATATTGATAGCGATGGATTCTTATTTGTCGTGGATCGCTTGAAAGAGCTCATTAAGTACAAGGGATATCAGGTTATTATTAACAATTCTATCTAATCTAATGCAGTATTCACAACCACGTTTCGATCACTTCAACTAAATAACCCAGAGATAAATTACTGTTTACCGACACACAATAGTAATAAAGATTATAAACGTAAATATTGTCAAACTCATGGAAGTTCTCTTGCTTTTGTTTTATGGTTATTGTTACAGGTCCCTCCAGCAGAACTAGAGGCTTTGTTGCTGACTCATCCTGCTATTGTAGACGCTGCTGTCATACCGTAAGCCTACCAAAGAATATAAATTAATTATAGTATCTTAATTTTAGTTTTTTTGTCTGCTTTGATTTATTTTTTATAATATGATGGTGACGAAAACCAGTATTCCTTCTTATCAATCAAGACCGCTGAATGTTTTGTGTGTATATTATATCAGGTATCCAAATAAGGAGGCTGGGCAGGTTCCAATGGCATATGTGGTAAGGAAGGCTGGAAGTAGCTTGTCAGGAAGCCAAGTTATGGATTTTGTTGCAGAGCAGGTTTGTTTGTTTGGAACACTTCTCAACAATGACACTCTTTTTTATTAGATGCTTGGGTTTTGATTTCAACTATGAGATTGAAAACTAAATTAATTTTTTTCAATATATAGGTGGCTCCATACAAAAGAATTCGAAAAGTGGCATTTATATCTTCTATACCCAAAAATCCATCTGGTAAAATTCTTCGGAAGGATCTTATCAAGCTAGCTACGTCTAAACTTTGA
Protein sequence

>MS.gene27109.t1

MEVNGENTNSRRVIDPRSGFCSSNSIFYSKRKPLPLPPNHSLDATTFISSRAHHGHIAFIDASTGHQFTYQQLWRAVDSVTSSLSNMGIRKGDVILLLSPNSIYFPVVCLSVMSLGAIITTTNPLNTSREIAKQIADSKPVLAFTTPQLLSKITQASPSLPIILMDTTSNSSSTTTLDEMMKKEPELSRVTERINQDDTATLLYSSGTTGPSKGVVSSHKNLIAMVQVVMARFGKEQETRETFICTVPMFHIYGLAVFAMGLLAMGSTIVVLSKFEMHDMLSSIEKFRATFLPLVPPILVAMLNNADAIKRKYDLSSLHTVVSGGAPLSKEVTEGFIEKYPNVSIFQGYGLTESSGIGASTESLEESRKYGTAGLVSASTEAMIVDTETAQPLPVNRTGELWLRGPTIMKGYFSNEEATTSTITPEGWLKTGDVCYIDSDGFLFVVDRLKELIKYKGYQVPPAELEALLLTHPAIVDAAVIPYPNKEAGQVPMAYVVRKAGSSLSGSQVMDFVAEQVAPYKRIRKVAFISSIPKNPSGKILRKDLIKLATSKL