AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09153

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09153.t1 MTR_4g057730 94.649 542 29 0 1 542 1 542 0.0 1053
MS.gene09153.t1 MTR_4g057730 94.692 471 25 0 1 471 1 471 0.0 920
MS.gene09153.t1 MTR_4g057730 94.444 450 25 0 1 450 1 450 0.0 877
MS.gene09153.t1 MTR_2g095510 72.509 542 146 2 1 542 1 539 0.0 820
MS.gene09153.t1 MTR_2g095510 68.889 450 137 2 1 450 1 447 0.0 652
MS.gene09153.t1 MTR_1g063110 58.240 534 216 6 12 539 13 545 0.0 612
MS.gene09153.t1 MTR_1g063120 57.565 542 211 6 12 539 13 549 0.0 605
MS.gene09153.t1 MTR_1g026310 45.338 547 289 5 3 542 17 560 1.86e-157 461
MS.gene09153.t1 MTR_1g090737 42.077 549 303 7 3 542 24 566 8.82e-149 439
MS.gene09153.t1 MTR_8g039720 45.290 552 282 8 4 542 15 559 3.93e-148 437
MS.gene09153.t1 MTR_2g105570 41.791 536 300 8 11 537 12 544 9.31e-143 423
MS.gene09153.t1 MTR_5g007640 43.094 543 284 8 11 540 55 585 8.38e-142 422
MS.gene09153.t1 MTR_5g027480 41.481 540 301 7 2 534 16 547 1.66e-138 412
MS.gene09153.t1 MTR_4g128337 42.185 531 299 5 11 537 17 543 3.88e-138 411
MS.gene09153.t1 MTR_4g005750 41.199 534 300 8 11 537 11 537 7.14e-134 400
MS.gene09153.t1 MTR_3g088870 42.495 513 275 6 30 535 52 551 1.58e-132 397
MS.gene09153.t1 MTR_1g073180 43.295 522 265 10 26 537 29 529 3.83e-129 388
MS.gene09153.t1 MTR_5g027500 40.395 557 303 11 6 542 21 568 1.57e-128 387
MS.gene09153.t1 MTR_3g088880 41.829 514 274 9 30 535 53 549 2.52e-128 386
MS.gene09153.t1 MTR_4g077900 41.079 482 274 6 67 542 1 478 2.12e-123 371
MS.gene09153.t1 MTR_3g460770 35.417 528 327 7 21 542 22 541 7.56e-120 364
MS.gene09153.t1 MTR_1g073180 42.032 502 260 10 26 517 51 531 3.56e-115 352
MS.gene09153.t1 MTR_1g026470 39.642 391 226 5 3 386 17 404 5.90e-82 261
MS.gene09153.t1 MTR_1g026430 39.691 388 224 5 3 383 17 401 2.64e-81 260
MS.gene09153.t1 MTR_1569s0010 29.887 532 333 9 35 534 4 527 1.30e-58 203
MS.gene09153.t1 MTR_3g035130 33.061 490 300 15 55 532 32 505 1.60e-57 200
MS.gene09153.t1 MTR_0803s0020 28.281 541 349 14 7 528 214 734 6.31e-49 182
MS.gene09153.t1 MTR_3g064420 26.606 545 357 10 30 540 17 552 5.71e-47 173
MS.gene09153.t1 MTR_3g035130 32.952 437 265 15 55 479 32 452 2.65e-46 169
MS.gene09153.t1 MTR_5g008820 27.290 535 350 14 30 540 17 536 9.44e-45 166
MS.gene09153.t1 MTR_2g044180 27.289 557 331 20 32 539 25 556 8.46e-43 161
MS.gene09153.t1 MTR_2g044180 31.888 392 216 16 181 539 92 465 4.01e-40 152
MS.gene09153.t1 MTR_4g057755 87.671 73 9 0 398 470 46 118 4.27e-39 139
MS.gene09153.t1 MTR_4g057755 76.667 60 13 1 222 281 1 59 4.35e-23 95.1
MS.gene09153.t1 MTR_4g011790 25.954 524 339 15 47 534 81 591 4.33e-39 151
MS.gene09153.t1 MTR_5g008800 26.335 543 347 13 30 540 17 538 4.65e-39 150
MS.gene09153.t1 MTR_4g013210 29.867 375 228 10 189 534 70 438 6.64e-39 148
MS.gene09153.t1 MTR_4g121600 26.381 561 349 21 23 539 16 556 1.92e-38 149
MS.gene09153.t1 MTR_5g076220 26.937 568 335 16 23 540 10 547 3.74e-38 148
MS.gene09153.t1 MTR_4g011790 25.856 526 341 15 47 536 81 593 1.31e-37 147
MS.gene09153.t1 MTR_4g011790 25.328 533 340 16 47 534 81 600 4.71e-37 145
MS.gene09153.t1 MTR_8g074040 24.408 549 366 14 23 540 52 582 1.92e-35 140
MS.gene09153.t1 MTR_5g076240 25.314 557 353 16 15 531 4 537 2.29e-35 140
MS.gene09153.t1 MTR_4g013210 28.375 363 208 11 189 534 70 397 6.14e-34 133
MS.gene09153.t1 MTR_4g013210 28.375 363 208 11 189 534 222 549 3.15e-33 133
MS.gene09153.t1 MTR_5g076200 25.586 555 357 16 23 539 10 546 3.78e-33 133
MS.gene09153.t1 MTR_2g030240 25.143 525 343 12 59 542 32 547 3.96e-33 133
MS.gene09153.t1 MTR_4g013210 29.630 351 212 10 189 510 222 566 5.82e-33 133
MS.gene09153.t1 MTR_8g074120 26.203 374 241 9 187 540 135 493 1.52e-31 128
MS.gene09153.t1 MTR_7g084850 24.715 526 340 13 54 534 202 716 1.59e-31 129
MS.gene09153.t1 MTR_0161s0050 24.955 557 360 14 23 539 10 548 1.95e-30 125
MS.gene09153.t1 MTR_1g104810 30.514 331 155 14 186 459 256 568 4.57e-29 122
MS.gene09153.t1 MTR_1g104810 30.514 331 155 14 186 459 189 501 5.84e-29 121
MS.gene09153.t1 MTR_8g074040 23.881 469 310 13 103 540 12 464 4.55e-28 117
MS.gene09153.t1 MTR_5g023730 24.432 528 360 17 30 531 19 533 1.22e-26 114
MS.gene09153.t1 MTR_2g030240 26.166 386 240 10 193 542 77 453 3.06e-26 112
MS.gene09153.t1 MTR_1g034360 24.125 514 290 19 32 464 49 543 4.31e-25 110
MS.gene09153.t1 MTR_5g009360 28.375 363 190 14 163 469 75 423 6.50e-23 102
MS.gene09153.t1 MTR_1g026350 43.284 134 73 2 3 135 17 148 1.13e-22 95.5
MS.gene09153.t1 MTR_5g009360 25.820 488 270 19 56 469 80 549 1.26e-22 102
MS.gene09153.t1 MTR_7g109830 27.390 387 202 17 189 506 221 597 1.27e-22 102
MS.gene09153.t1 MTR_1g086600 25.389 386 213 12 189 506 243 621 2.13e-22 101
MS.gene09153.t1 MTR_4g068670 23.063 542 304 21 6 464 30 541 1.36e-21 99.4
MS.gene09153.t1 MTR_5g009350 26.608 342 183 12 186 470 220 550 5.34e-21 97.1
MS.gene09153.t1 MTR_1054s0020 23.674 528 366 15 13 529 258 759 5.94e-21 97.4
MS.gene09153.t1 MTR_5g009350 27.353 340 181 12 186 469 220 549 9.84e-21 96.7
MS.gene09153.t1 MTR_5g009350 27.353 340 181 12 186 469 220 549 1.15e-20 96.3
MS.gene09153.t1 MTR_5g009350 26.393 341 183 12 186 469 220 549 1.23e-20 95.9
MS.gene09153.t1 MTR_3g084470 30.328 244 135 6 332 542 469 710 1.74e-20 95.9
MS.gene09153.t1 MTR_3g084470 25.738 237 150 7 35 248 83 316 1.08e-13 74.3
MS.gene09153.t1 MTR_8g083170 36.842 171 91 4 311 470 459 623 1.79e-20 95.9
MS.gene09153.t1 MTR_4g013210 26.543 324 182 11 189 505 222 496 2.91e-20 94.4
MS.gene09153.t1 MTR_3g084450 28.333 240 136 5 337 542 270 507 9.43e-19 89.7
MS.gene09153.t1 MTR_3g084450 27.755 245 141 5 332 542 473 715 1.62e-18 89.7
MS.gene09153.t1 MTR_4g013210 28.992 238 145 7 189 408 222 453 5.99e-16 80.9
MS.gene09153.t1 MTR_8g099420 33.898 177 102 7 289 460 375 541 2.04e-15 79.7
MS.gene09153.t1 MTR_3g088890 45.312 64 35 0 444 507 10 73 2.62e-15 72.0
MS.gene09153.t1 MTR_1g030290 31.776 214 110 12 312 509 426 619 7.48e-14 74.7
MS.gene09153.t1 MTR_2g098490 24.819 415 240 17 182 537 640 1041 2.23e-13 73.9
MS.gene09153.t1 MTR_1586s0010 21.096 365 238 7 66 399 81 426 3.62e-13 72.0
MS.gene09153.t1 MTR_3g087980 27.559 254 136 12 296 517 412 649 1.38e-11 67.8
MS.gene09153.t1 MTR_4g066130 22.922 397 255 15 189 537 651 1044 6.37e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09153.t1 AT4G05160 66.850 546 175 2 1 542 1 544 0.0 760
MS.gene09153.t1 AT1G20510 44.946 554 271 8 3 540 7 542 1.04e-156 458
MS.gene09153.t1 AT1G20500 44.685 555 275 10 3 542 13 550 1.45e-147 435
MS.gene09153.t1 AT1G65060 43.097 536 286 8 11 537 34 559 4.76e-144 427
MS.gene09153.t1 AT3G21240 42.037 540 296 8 7 537 18 549 2.77e-143 424
MS.gene09153.t1 AT1G20480 42.703 555 292 10 2 542 23 565 5.51e-141 419
MS.gene09153.t1 AT5G38120 42.626 556 284 10 3 542 14 550 1.89e-139 414
MS.gene09153.t1 AT4G19010 41.360 544 287 11 7 534 27 554 4.70e-136 406
MS.gene09153.t1 AT5G63380 41.621 543 285 12 10 534 22 550 4.34e-135 404
MS.gene09153.t1 AT1G51680 40.951 547 307 7 2 537 15 556 1.38e-134 402
MS.gene09153.t1 AT1G20510 43.093 485 242 8 3 471 7 473 1.51e-124 374
MS.gene09153.t1 AT1G51680 40.310 516 292 7 2 506 15 525 3.21e-122 370
MS.gene09153.t1 AT5G38120 41.346 520 270 10 3 506 14 514 3.44e-122 369
MS.gene09153.t1 AT3G21230 38.828 546 313 8 7 537 24 563 2.12e-121 369
MS.gene09153.t1 AT3G21240 40.842 475 264 8 7 472 18 484 1.39e-116 354
MS.gene09153.t1 AT3G21240 40.842 475 264 8 7 472 18 484 1.39e-116 354
MS.gene09153.t1 AT1G62940 36.735 539 320 9 11 542 12 536 1.18e-115 353
MS.gene09153.t1 AT1G20510 41.469 463 237 8 3 449 7 451 4.56e-111 339
MS.gene09153.t1 AT3G21230 38.104 538 306 11 7 529 24 549 1.75e-109 337
MS.gene09153.t1 AT1G51680 40.000 480 272 7 2 470 15 489 5.38e-109 334
MS.gene09153.t1 AT1G65060 41.111 450 246 8 11 451 34 473 1.87e-108 333
MS.gene09153.t1 AT5G38120 40.412 485 254 10 3 471 14 479 7.76e-107 328
MS.gene09153.t1 AT5G38120 40.412 485 254 10 3 471 14 479 7.76e-107 328
MS.gene09153.t1 AT3G21230 37.996 479 276 8 7 470 24 496 7.97e-98 306
MS.gene09153.t1 AT1G20490 38.051 431 237 13 3 418 13 428 1.60e-82 264
MS.gene09153.t1 AT1G20490 38.051 431 237 13 3 418 13 428 1.60e-82 264
MS.gene09153.t1 AT3G48990 31.746 504 315 14 41 532 18 504 6.15e-59 204
MS.gene09153.t1 AT3G21230 33.741 409 250 7 7 400 24 426 8.75e-58 199
MS.gene09153.t1 AT1G65890 25.887 564 361 17 18 538 5 554 1.90e-47 174
MS.gene09153.t1 AT1G77240 27.207 555 357 15 19 540 3 543 1.18e-46 171
MS.gene09153.t1 AT1G65880 25.044 563 368 16 18 538 5 555 2.24e-45 168
MS.gene09153.t1 AT1G66120 26.316 551 366 13 19 538 6 547 4.03e-42 159
MS.gene09153.t1 AT1G21540 27.143 560 356 18 19 540 3 548 6.66e-42 158
MS.gene09153.t1 AT3G16170 25.859 553 334 17 34 534 72 600 1.70e-40 155
MS.gene09153.t1 AT1G30520 27.308 509 327 15 64 534 42 545 1.03e-39 152
MS.gene09153.t1 AT1G30520 27.308 509 327 15 64 534 42 545 1.03e-39 152
MS.gene09153.t1 AT1G68270 24.910 558 336 18 19 536 6 520 1.75e-36 142
MS.gene09153.t1 AT1G21530 25.448 558 367 20 19 540 3 547 9.49e-36 140
MS.gene09153.t1 AT1G21530 26.025 561 358 23 19 540 3 545 2.29e-35 139
MS.gene09153.t1 AT5G16340 25.397 567 360 20 15 541 4 547 2.46e-35 139
MS.gene09153.t1 AT5G16370 25.136 553 371 18 15 537 4 543 1.49e-34 137
MS.gene09153.t1 AT1G20560 24.182 550 354 16 30 540 19 544 2.60e-34 137
MS.gene09153.t1 AT1G76290 26.079 533 363 16 27 539 14 535 3.45e-34 136
MS.gene09153.t1 AT1G75960 24.505 555 371 15 15 537 4 542 3.52e-34 136
MS.gene09153.t1 AT5G27600 26.527 524 291 20 11 457 63 569 1.91e-32 132
MS.gene09153.t1 AT2G17650 24.000 575 367 19 12 538 45 597 5.30e-32 130
MS.gene09153.t1 AT3G16910 24.779 565 336 22 32 539 27 559 5.64e-32 130
MS.gene09153.t1 AT5G36880 24.621 528 338 16 54 534 147 661 8.46e-31 127
MS.gene09153.t1 AT5G36880 24.621 528 338 16 54 534 147 661 8.46e-31 127
MS.gene09153.t1 AT5G36880 24.621 528 338 16 54 534 147 661 8.46e-31 127
MS.gene09153.t1 AT5G36880 24.621 528 338 16 54 534 147 661 8.46e-31 127
MS.gene09153.t1 AT5G36880 24.621 528 338 16 54 534 197 711 1.12e-30 127
MS.gene09153.t1 AT1G30520 25.995 427 284 13 64 466 42 460 2.62e-30 124
MS.gene09153.t1 AT1G30520 25.995 427 284 13 64 466 42 460 2.62e-30 124
MS.gene09153.t1 AT1G20560 26.316 380 233 11 187 540 108 466 2.00e-29 121
MS.gene09153.t1 AT1G64400 25.287 522 305 23 11 457 27 538 2.09e-26 114
MS.gene09153.t1 AT3G05970 30.063 316 172 10 186 457 259 569 2.25e-24 107
MS.gene09153.t1 AT2G47240 26.402 428 237 17 179 535 209 629 1.57e-23 105
MS.gene09153.t1 AT2G47240 26.402 428 237 17 179 535 209 629 1.57e-23 105
MS.gene09153.t1 AT2G47240 26.402 428 237 17 179 535 209 629 1.57e-23 105
MS.gene09153.t1 AT2G47240 26.402 428 237 17 179 535 150 570 1.62e-23 105
MS.gene09153.t1 AT5G36880 23.861 461 294 15 54 470 147 594 4.05e-23 103
MS.gene09153.t1 AT1G49430 28.021 389 205 15 186 506 221 602 4.88e-23 103
MS.gene09153.t1 AT4G23850 22.674 516 310 17 9 457 45 538 2.93e-20 95.1
MS.gene09153.t1 AT4G11030 24.897 486 278 19 56 470 81 550 5.42e-19 91.3
MS.gene09153.t1 AT3G23790 28.904 301 171 10 279 542 423 717 2.69e-18 89.0
MS.gene09153.t1 AT4G14070 38.000 150 80 4 332 470 490 637 5.01e-18 88.2
MS.gene09153.t1 AT4G14070 23.932 234 155 8 35 247 105 336 5.30e-12 68.9
MS.gene09153.t1 AT1G77590 34.104 173 89 9 312 469 276 438 1.74e-13 73.6
MS.gene09153.t1 AT1G77590 34.104 173 89 9 312 469 422 584 2.74e-13 73.2
MS.gene09153.t1 AT5G35930 25.195 385 240 12 181 529 95 467 2.14e-11 67.4
MS.gene09153.t1 AT2G04350 33.766 154 80 7 334 473 472 617 2.23e-11 67.0
MS.gene09153.t1 AT2G04350 33.766 154 80 7 334 473 472 617 2.23e-11 67.0
MS.gene09153.t1 AT5G35930 25.195 385 240 12 181 529 187 559 2.66e-11 67.0

Find 134 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATTATATCTTATATTT+TGG 0.116812 4.3:+43371494 None:intergenic
GGTACAGTGATAACTAATTT+AGG 0.209914 4.3:+43374362 None:intergenic
GGAGGGCTAGAGAAGGTCTT+AGG 0.217215 4.3:+43374699 None:intergenic
AAGGAGATGTTTGGATTGTT+TGG 0.225138 4.3:+43374677 None:intergenic
TCAGCTGGAATGTTGGTTTC+AGG 0.248160 4.3:-43373149 MS.gene09153:CDS
TCAGAAGTTTATTGCTAATC+AGG 0.249995 4.3:-43371801 MS.gene09153:intron
ACGACAGCATCTTGTATTTC+AGG 0.265567 4.3:+43372129 None:intergenic
TTATCACTGTACCTGAATTA+TGG 0.271891 4.3:-43374352 MS.gene09153:CDS
GTTGAGAGAAGGAGAGGGTT+TGG 0.278616 4.3:+43374585 None:intergenic
TTCACTTTGCTCCATAATTC+AGG 0.281829 4.3:+43374341 None:intergenic
AATGGGGAGGTTTGAGTTAG+AGG 0.302369 4.3:-43373693 MS.gene09153:CDS
TTTCTTCATTGAAGAGTATT+GGG 0.309637 4.3:-43373568 MS.gene09153:CDS
ACCTCATGTTACTATTTATC+AGG 0.312959 4.3:-43373492 MS.gene09153:intron
ATTGGAATCCTTGATTTGTT+TGG 0.316951 4.3:+43374385 None:intergenic
TTGAGAGAAGGAGAGGGTTT+GGG 0.319270 4.3:+43374586 None:intergenic
GATCAAATACAAAGGTTATC+AGG 0.342076 4.3:-43372454 MS.gene09153:intron
TTGCCGATGTTTCATGTGTT+TGG 0.353705 4.3:-43373767 MS.gene09153:CDS
AGCGTAGGTATTCGTCATTC+TGG 0.360952 4.3:-43373173 MS.gene09153:CDS
GCCACTCCCTCCAAAACAAT+TGG 0.363709 4.3:-43373090 MS.gene09153:CDS
TACAGGAAACTGGATGTTAT+TGG 0.371299 4.3:+43374481 None:intergenic
GGGTCTGGTGCTGCTCCTCT+TGG 0.372270 4.3:-43373548 MS.gene09153:CDS
AATGAGACTAAGGAGATGTT+TGG 0.381371 4.3:+43374668 None:intergenic
AGGAGATGTTTGGATTGTTT+GGG 0.382762 4.3:+43374678 None:intergenic
TCTCTTTCAATGCAGAAATC+TGG 0.384408 4.3:-43374749 None:intergenic
CCTGAGTTGATGATAATTGC+AGG 0.391344 4.3:+43374299 None:intergenic
GCCACCGATGATTCTTGCTT+TGG 0.397468 4.3:-43373621 MS.gene09153:CDS
ACCTCACCAGCCTCGTCATC+AGG 0.398355 4.3:+43371878 None:intergenic
CCACTCCCTCCAAAACAATT+GGG 0.408832 4.3:-43373089 MS.gene09153:CDS
AGGAAGTACGGTGGTTTCAA+TGG 0.410372 4.3:-43373711 MS.gene09153:CDS
GTTTCTTCATTGAAGAGTAT+TGG 0.413086 4.3:-43373569 MS.gene09153:CDS
CTAGGAAACATACAGGAAAC+TGG 0.415218 4.3:+43374471 None:intergenic
TCCTCGGACAGCAAACTGTT+GGG 0.419851 4.3:+43371830 None:intergenic
TCCTCTTGGGAAGGAATTGA+TGG 0.427485 4.3:-43373534 MS.gene09153:CDS
AATTTACCTTGCATCATGTT+GGG 0.436629 4.3:+43373047 None:intergenic
ATTCGTCATTCTGGCTCAGC+TGG 0.439809 4.3:-43373164 MS.gene09153:CDS
TGGGTACATACAGGAGATCT+TGG 0.444644 4.3:-43372528 MS.gene09153:CDS
TTCCTCGGACAGCAAACTGT+TGG 0.447080 4.3:+43371829 None:intergenic
GGTCTGGTGCTGCTCCTCTT+GGG 0.450711 4.3:-43373547 MS.gene09153:CDS
TTCTGGCTCAGCTGGAATGT+TGG 0.451563 4.3:-43373156 MS.gene09153:CDS
GGAAGTACGGTGGTTTCAAT+GGG 0.453310 4.3:-43373710 MS.gene09153:CDS
AGTGTTCCTAAGACAGCTTC+AGG 0.454588 4.3:-43371555 MS.gene09153:CDS
AGTATAGAGTGACAAATTTG+TGG 0.464020 4.3:-43373649 MS.gene09153:CDS
GAACGAACAACATAGGCAAT+TGG 0.465645 4.3:+43371854 None:intergenic
CAATTTACCTTGCATCATGT+TGG 0.472409 4.3:+43373046 None:intergenic
GATGTTTCATGTGTTTGGAT+TGG 0.473581 4.3:-43373762 MS.gene09153:CDS
ATCTTGTACTATTAGGGGTA+TGG 0.475615 4.3:-43373236 MS.gene09153:intron
GTTTCATGTGTTTGGATTGG+CGG 0.478530 4.3:-43373759 MS.gene09153:CDS
CGACAGCATCTTGTATTTCA+GGG 0.482401 4.3:+43372130 None:intergenic
ATGGGGAGGTTTGAGTTAGA+GGG 0.485043 4.3:-43373692 MS.gene09153:CDS
TCATTGAAGAGTATTGGGTC+TGG 0.485714 4.3:-43373563 MS.gene09153:CDS
CTGTACTCATCAGGAACTAC+AGG 0.487926 4.3:-43373899 MS.gene09153:CDS
AACATGGACGATGATTTAGC+AGG 0.490835 4.3:-43373818 MS.gene09153:CDS
AGGGACCTGTATATTCCATC+TGG 0.491867 4.3:+43374719 None:intergenic
TGGCGAAACAGAGTGTGGTT+GGG 0.494845 4.3:-43373601 MS.gene09153:CDS
TTATAACATACGCGCAGCTA+AGG 0.495514 4.3:-43373736 MS.gene09153:CDS
TTCCTAGCTGCCACCTCCAT+CGG 0.495897 4.3:-43374455 MS.gene09153:CDS
AGTGATAACTAATTTAGGAT+TGG 0.497297 4.3:+43374367 None:intergenic
GCGGCACTTCTGTACTCATC+AGG 0.505987 4.3:-43373908 MS.gene09153:CDS
GTATAGAGTGACAAATTTGT+GGG 0.509344 4.3:-43373648 MS.gene09153:CDS
GAGGGCTAGAGAAGGTCTTA+GGG 0.511707 4.3:+43374700 None:intergenic
TACGATCAACAACATAAAGT+TGG 0.524847 4.3:+43372487 None:intergenic
CCTGCAATTATCATCAACTC+AGG 0.525372 4.3:-43374299 MS.gene09153:intron
TCCTGATGACGAGGCTGGTG+AGG 0.526657 4.3:-43371879 MS.gene09153:CDS
AGATTTCCTGATGACGAGGC+TGG 0.527482 4.3:-43371884 MS.gene09153:CDS
GCACACAGTTTACTCAACCT+TGG 0.529470 4.3:-43374539 MS.gene09153:CDS
AAACTTCTGAATTTCTTCCT+CGG 0.530812 4.3:+43371814 None:intergenic
TGGTGCTGCTCCTCTTGGGA+AGG 0.534764 4.3:-43373543 MS.gene09153:CDS
TTTGAGTTGAGAGAAGGAGA+GGG 0.536638 4.3:+43374580 None:intergenic
TATCTAATAGGTAATGCTAC+GGG 0.537453 4.3:-43374025 MS.gene09153:intron
GAATGACGAATACCTACGCT+TGG 0.539411 4.3:+43373176 None:intergenic
TGTTATTGGTGGAGAGGATG+AGG 0.539934 4.3:+43374495 None:intergenic
GGAACTCTCCAAACAAATCA+AGG 0.541312 4.3:-43374393 MS.gene09153:CDS
AATGAGGGGTGGGTACATAC+AGG 0.542233 4.3:-43372537 MS.gene09153:CDS
ATATCTAATAGGTAATGCTA+CGG 0.542243 4.3:-43374026 MS.gene09153:intron
GTACAGAAGTGCCGCTGTAT+CGG 0.543298 4.3:+43373916 None:intergenic
AGATGTTTGGATTGTTTGGG+AGG 0.543747 4.3:+43374681 None:intergenic
TGTGAAGAGGAATGAGACTA+AGG 0.543904 4.3:+43374658 None:intergenic
AAAACAAACTTTAGACAATG+AGG 0.546586 4.3:-43372553 MS.gene09153:CDS
AGAAGATCTTCAAGTTCTGC+GGG 0.547646 4.3:+43372162 None:intergenic
ATAGTCCAGATGGAATATAC+AGG 0.549923 4.3:-43374724 MS.gene09153:CDS
GATGTTTGGATTGTTTGGGA+GGG 0.551181 4.3:+43374682 None:intergenic
GAGGCGTTGATTGAATCAGC+AGG 0.553384 4.3:-43373977 MS.gene09153:CDS
TTGGCGAAACAGAGTGTGGT+TGG 0.557745 4.3:-43373602 MS.gene09153:CDS
AAATCTGGTTATAGTCCAGA+TGG 0.559141 4.3:-43374734 MS.gene09153:CDS
ACCCAACAGTTTGCTGTCCG+AGG 0.559615 4.3:-43371831 MS.gene09153:CDS
CACCATTCAAAAGATTGCGA+AGG 0.561043 4.3:-43371593 MS.gene09153:CDS
ACATGGACGATGATTTAGCA+GGG 0.561737 4.3:-43373817 MS.gene09153:CDS
GGAAGAGTCTGCGTCGATGA+GGG 0.562446 4.3:+43374607 None:intergenic
TGGGGAGGTTTGAGTTAGAG+GGG 0.562823 4.3:-43373691 MS.gene09153:CDS
AAACAAACTTTAGACAATGA+GGG 0.563086 4.3:-43372552 MS.gene09153:CDS
GCAGCTAAGGAGAGGAAGTA+CGG 0.565977 4.3:-43373723 MS.gene09153:CDS
ACCATTCAAAAGATTGCGAA+GGG 0.569226 4.3:-43371592 MS.gene09153:CDS
AACTTTAGACAATGAGGGGT+GGG 0.570046 4.3:-43372547 MS.gene09153:CDS
CACCGATGGAGGTGGCAGCT+AGG 0.571967 4.3:+43374453 None:intergenic
GGGAAGAGTCTGCGTCGATG+AGG 0.577257 4.3:+43374606 None:intergenic
CACTCCCTCCAAAACAATTG+GGG 0.578348 4.3:-43373088 MS.gene09153:CDS
CGAAGCTCAAATAGTGAGTG+TGG 0.579940 4.3:-43373123 MS.gene09153:CDS
AGGAAACTGGATGTTATTGG+TGG 0.583013 4.3:+43374484 None:intergenic
GTTGGGTGAACGAACAACAT+AGG 0.583728 4.3:+43371847 None:intergenic
GTGGCAGCTAGGAAACATAC+AGG 0.584515 4.3:+43374464 None:intergenic
CTATAGATTTCCTGATGACG+AGG 0.586638 4.3:-43371888 MS.gene09153:intron
GACTATTGCACCGATGGAGG+TGG 0.596107 4.3:+43374445 None:intergenic
AAGAAGATCTTCAAGTTCTG+CGG 0.597098 4.3:+43372161 None:intergenic
AAGAAGAGACGCTTGTGAAG+AGG 0.603096 4.3:+43374645 None:intergenic
TTCGCCAAAGCAAGAATCAT+CGG 0.605055 4.3:+43373617 None:intergenic
TTGTTTGGAGAGTTCCGCAA+CGG 0.605682 4.3:+43374400 None:intergenic
GGAACTACAGGAGTGAGCAA+AGG 0.615795 4.3:-43373887 MS.gene09153:CDS
AAATACTCTTTACACCGTTG+CGG 0.620177 4.3:-43374414 MS.gene09153:CDS
TCTATCGAAAATCCAAGCGT+AGG 0.620944 4.3:-43373188 MS.gene09153:CDS
TTGTTTGGGAGGGCTAGAGA+AGG 0.621430 4.3:+43374692 None:intergenic
ACTGGATGTTATTGGTGGAG+AGG 0.622948 4.3:+43374489 None:intergenic
AAACTTTAGACAATGAGGGG+TGG 0.625674 4.3:-43372548 MS.gene09153:CDS
CGAGGTCCCAACATGATGCA+AGG 0.625710 4.3:-43373053 MS.gene09153:intron
GTACGGTGGTTTCAATGGGG+AGG 0.625711 4.3:-43373706 MS.gene09153:CDS
TCCATCAATTCCTTCCCAAG+AGG 0.631113 4.3:+43373533 None:intergenic
AACAAACTTTAGACAATGAG+GGG 0.631532 4.3:-43372551 MS.gene09153:CDS
AATCCAAACACATGAAACAT+CGG 0.641573 4.3:+43373764 None:intergenic
TGCTTTGGCGAAACAGAGTG+TGG 0.643161 4.3:-43373606 MS.gene09153:CDS
CATGGACGATGATTTAGCAG+GGG 0.652854 4.3:-43373816 MS.gene09153:CDS
TCTTGGGAAGGAATTGATGG+AGG 0.654495 4.3:-43373531 MS.gene09153:CDS
AAAGAACTGATCAAATACAA+AGG 0.656643 4.3:-43372462 MS.gene09153:CDS
ACATACGCGCAGCTAAGGAG+AGG 0.659076 4.3:-43373731 MS.gene09153:CDS
ACCTGATAAATAGTAACATG+AGG 0.666648 4.3:+43373491 None:intergenic
ACTCCCTCCAAAACAATTGG+GGG 0.667004 4.3:-43373087 MS.gene09153:CDS
AGGTTATTATAACAATCCAG+AGG 0.669013 4.3:-43372580 MS.gene09153:CDS
GGTGACTATTGCACCGATGG+AGG 0.673270 4.3:+43374442 None:intergenic
AGTGGTGACTATTGCACCGA+TGG 0.675842 4.3:+43374439 None:intergenic
GCCAAAGCAAGAATCATCGG+TGG 0.703214 4.3:+43373620 None:intergenic
AGCGGCGTTAATGATTAACA+TGG 0.703668 4.3:-43373834 MS.gene09153:CDS
GAAGTACGGTGGTTTCAATG+GGG 0.715971 4.3:-43373709 MS.gene09153:CDS
TTTGAAACAGACCGATACAG+CGG 0.717255 4.3:-43373927 MS.gene09153:CDS
GGTGTAAAGAGTATTTGCAG+TGG 0.726670 4.3:+43374421 None:intergenic
ACACAAGAACTTCATCGCAG+CGG 0.736714 4.3:-43373852 MS.gene09153:CDS
GCTAAGGAGAGGAAGTACGG+TGG 0.774505 4.3:-43373720 MS.gene09153:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATAGAGAAATATCAAT+TGG + chr4.3:43374314-43374333 None:intergenic 15.0%
!! GTTTAAAAATTAGTTGAAAT+TGG + chr4.3:43374619-43374638 None:intergenic 15.0%
!! TAACAAACTTTATATCTAAT+AGG - chr4.3:43372209-43372228 MS.gene09153:intron 15.0%
!! AATAACCTGAAATATTGTAT+AGG + chr4.3:43373654-43373673 None:intergenic 20.0%
!! ACAATATAATTGTATTCTGT+TGG + chr4.3:43373274-43373293 None:intergenic 20.0%
!! ACCATTAAAAACATAAATAC+AGG + chr4.3:43374577-43374596 None:intergenic 20.0%
!! ATTGATTATCTTGTACTATT+AGG - chr4.3:43373003-43373022 MS.gene09153:intron 20.0%
!! CCATTAAAAACATAAATACA+GGG + chr4.3:43374576-43374595 None:intergenic 20.0%
!! GATCTTAAGAAATATTAATG+AGG + chr4.3:43373967-43373986 None:intergenic 20.0%
!! TTAATATTTCTTAAGATCGA+TGG - chr4.3:43373969-43373988 MS.gene09153:CDS 20.0%
!! TTGATTATCTTGTACTATTA+GGG - chr4.3:43373004-43373023 MS.gene09153:intron 20.0%
!!! AATTAAAGTATCTTGATCAT+TGG - chr4.3:43372896-43372915 MS.gene09153:intron 20.0%
!!! TAATTAGTTGTTTTCTTCAT+TGG - chr4.3:43372064-43372083 MS.gene09153:intron 20.0%
!!! TAATTTTTAAACACGCTTAT+AGG - chr4.3:43374627-43374646 MS.gene09153:CDS 20.0%
!!! TGTATTTATGTTTTTAATGG+TGG - chr4.3:43374576-43374595 MS.gene09153:CDS 20.0%
!!! TGTTATTTAAAAGCATGAAA+AGG + chr4.3:43373485-43373504 None:intergenic 20.0%
! AAAACAAACTTTAGACAATG+AGG - chr4.3:43373693-43373712 MS.gene09153:CDS 25.0%
! AAACAAACTTTAGACAATGA+GGG - chr4.3:43373694-43373713 MS.gene09153:CDS 25.0%
! AAAGAACTGATCAAATACAA+AGG - chr4.3:43373784-43373803 MS.gene09153:CDS 25.0%
! AATCACTTAGAGTTATTTGT+AGG + chr4.3:43373249-43373268 None:intergenic 25.0%
! ACCATATAAATATACTGTGA+TGG - chr4.3:43374272-43374291 MS.gene09153:intron 25.0%
! ATATCTAATAGGTAATGCTA+CGG - chr4.3:43372220-43372239 MS.gene09153:intron 25.0%
! ATGTTACTATTTATCAGGTA+AGG - chr4.3:43372759-43372778 MS.gene09153:intron 25.0%
! GTATAGGAAAAGTTTCATAT+GGG + chr4.3:43373638-43373657 None:intergenic 25.0%
! TAAAATCGCTATCTTTAATC+CGG + chr4.3:43372862-43372881 None:intergenic 25.0%
! TATATCTCCAACTTTATGAT+TGG - chr4.3:43373599-43373618 MS.gene09153:CDS 25.0%
! TCCATCACAGTATATTTATA+TGG + chr4.3:43374276-43374295 None:intergenic 25.0%
! TGATTATCTTGTACTATTAG+GGG - chr4.3:43373005-43373024 MS.gene09153:intron 25.0%
! TGTATAGGAAAAGTTTCATA+TGG + chr4.3:43373639-43373658 None:intergenic 25.0%
! TTACATTCCAATCATAAAGT+TGG + chr4.3:43373609-43373628 None:intergenic 25.0%
!! AGTGATAACTAATTTAGGAT+TGG + chr4.3:43371882-43371901 None:intergenic 25.0%
!! ATATCCTAACTTGTAACAAT+TGG - chr4.3:43374209-43374228 MS.gene09153:intron 25.0%
!! CTTTTCCTATACAATATTTC+AGG - chr4.3:43373646-43373665 MS.gene09153:CDS 25.0%
!!! ATTGTCTAAAGTTTGTTTTG+TGG + chr4.3:43373692-43373711 None:intergenic 25.0%
!!! CCCTGTATTTATGTTTTTAA+TGG - chr4.3:43374573-43374592 MS.gene09153:CDS 25.0%
!!! TTGTCTCTTTGAAATTAACT+AGG - chr4.3:43374056-43374075 MS.gene09153:intron 25.0%
!!! TTTCTTCATTGAAGAGTATT+GGG - chr4.3:43372678-43372697 MS.gene09153:intron 25.0%
AAACTTCTGAATTTCTTCCT+CGG + chr4.3:43374435-43374454 None:intergenic 30.0%
AACAAACTTTAGACAATGAG+GGG - chr4.3:43373695-43373714 MS.gene09153:CDS 30.0%
AAGTAAACATAACAGTGACA+AGG + chr4.3:43373898-43373917 None:intergenic 30.0%
AATCCAAACACATGAAACAT+CGG + chr4.3:43372485-43372504 None:intergenic 30.0%
AATTTACCTTGCATCATGTT+GGG + chr4.3:43373202-43373221 None:intergenic 30.0%
ACCTCATGTTACTATTTATC+AGG - chr4.3:43372754-43372773 MS.gene09153:intron 30.0%
ACCTGATAAATAGTAACATG+AGG + chr4.3:43372758-43372777 None:intergenic 30.0%
AGGTTATTATAACAATCCAG+AGG - chr4.3:43373666-43373685 MS.gene09153:CDS 30.0%
AGTATAGAGTGACAAATTTG+TGG - chr4.3:43372597-43372616 MS.gene09153:intron 30.0%
CAAACCAATTGTTACAAGTT+AGG + chr4.3:43374216-43374235 None:intergenic 30.0%
GATATAAAAACTGCAAGACT+TGG + chr4.3:43373585-43373604 None:intergenic 30.0%
GATCAAATACAAAGGTTATC+AGG - chr4.3:43373792-43373811 MS.gene09153:CDS 30.0%
GGTACAGTGATAACTAATTT+AGG + chr4.3:43371887-43371906 None:intergenic 30.0%
GTATAGAGTGACAAATTTGT+GGG - chr4.3:43372598-43372617 MS.gene09153:intron 30.0%
TACGATCAACAACATAAAGT+TGG + chr4.3:43373762-43373781 None:intergenic 30.0%
TATCTAATAGGTAATGCTAC+GGG - chr4.3:43372221-43372240 MS.gene09153:intron 30.0%
TCAGAAGTTTATTGCTAATC+AGG - chr4.3:43374445-43374464 MS.gene09153:CDS 30.0%
TGTTATGTTTACTTGAGCAT+TGG - chr4.3:43373904-43373923 MS.gene09153:CDS 30.0%
TTAGTCTAACTATAACCAAC+TGG + chr4.3:43372047-43372066 None:intergenic 30.0%
TTATCACTGTACCTGAATTA+TGG - chr4.3:43371894-43371913 MS.gene09153:intron 30.0%
TTGATCAAGAGAAGTTTCAT+TGG + chr4.3:43373433-43373452 None:intergenic 30.0%
TTGGAAAGATGATATGGTAT+TGG - chr4.3:43373923-43373942 MS.gene09153:CDS 30.0%
! AGTGTTCTTGTTATGCTTTA+AGG - chr4.3:43373300-43373319 MS.gene09153:intron 30.0%
! GTTTCTTCATTGAAGAGTAT+TGG - chr4.3:43372677-43372696 MS.gene09153:intron 30.0%
! TATCATGCAAGTTTTACTCA+CGG + chr4.3:43374147-43374166 None:intergenic 30.0%
! TGGATTTTCGATAGAAACAA+CGG + chr4.3:43373053-43373072 None:intergenic 30.0%
! TGGCTATATATTTGTTGCAT+TGG - chr4.3:43372084-43372103 MS.gene09153:intron 30.0%
!! ATTGGAATCCTTGATTTGTT+TGG + chr4.3:43371864-43371883 None:intergenic 30.0%
!! TAAATCATTTTTATCCCAGC+TGG + chr4.3:43372976-43372995 None:intergenic 30.0%
!!! CTTGGATATTTTGATGAGAT+CGG - chr4.3:43373736-43373755 MS.gene09153:CDS 30.0%
!!! TACGAGGATTATTTTTGACA+AGG - chr4.3:43374013-43374032 MS.gene09153:CDS 30.0%
AAAGTCTCTGTAGAGTACAA+TGG - chr4.3:43373545-43373564 MS.gene09153:CDS 35.0%
AAATACTCTTTACACCGTTG+CGG - chr4.3:43371832-43371851 MS.gene09153:CDS 35.0%
AAATCTGGTTATAGTCCAGA+TGG - chr4.3:43371512-43371531 MS.gene09153:CDS 35.0%
AAGGAGATGTTTGGATTGTT+TGG + chr4.3:43371572-43371591 None:intergenic 35.0%
AATGAGACTAAGGAGATGTT+TGG + chr4.3:43371581-43371600 None:intergenic 35.0%
ACATAACAGTGACAAGGATT+TGG + chr4.3:43373892-43373911 None:intergenic 35.0%
ACCATTCAAAAGATTGCGAA+GGG - chr4.3:43374654-43374673 MS.gene09153:CDS 35.0%
ACCGACTGATCTTGATAAAA+CGG + chr4.3:43372839-43372858 None:intergenic 35.0%
AGGAGATGTTTGGATTGTTT+GGG + chr4.3:43371571-43371590 None:intergenic 35.0%
ATAGTCCAGATGGAATATAC+AGG - chr4.3:43371522-43371541 MS.gene09153:CDS 35.0%
ATCTTGTACTATTAGGGGTA+TGG - chr4.3:43373010-43373029 MS.gene09153:intron 35.0%
CAATGACACATTCAGATCAT+TGG + chr4.3:43372933-43372952 None:intergenic 35.0%
CAATTTACCTTGCATCATGT+TGG + chr4.3:43373203-43373222 None:intergenic 35.0%
CATTGCATTGAACCTATTTG+AGG - chr4.3:43372948-43372967 MS.gene09153:intron 35.0%
GATGTTTCATGTGTTTGGAT+TGG - chr4.3:43372484-43372503 MS.gene09153:CDS 35.0%
GCATGATAGTTTACGTTTGA+GGG - chr4.3:43374159-43374178 MS.gene09153:intron 35.0%
TAATCTTGATCGGACAGTTA+GGG - chr4.3:43372782-43372801 MS.gene09153:intron 35.0%
TACAGGAAACTGGATGTTAT+TGG + chr4.3:43371768-43371787 None:intergenic 35.0%
TCAGGTAAGGTAATCTTGAT+CGG - chr4.3:43372772-43372791 MS.gene09153:intron 35.0%
TGCATGATAGTTTACGTTTG+AGG - chr4.3:43374158-43374177 MS.gene09153:intron 35.0%
TTCACTTTGCTCCATAATTC+AGG + chr4.3:43371908-43371927 None:intergenic 35.0%
! AAGAAGATCTTCAAGTTCTG+CGG + chr4.3:43374088-43374107 None:intergenic 35.0%
! ATCATGCAAGTTTTACTCAC+GGG + chr4.3:43374146-43374165 None:intergenic 35.0%
! ATTTTTCCTGAAGCTGTCTT+AGG + chr4.3:43374700-43374719 None:intergenic 35.0%
! CATATTTCCCCCAATTGTTT+TGG + chr4.3:43373169-43373188 None:intergenic 35.0%
! GTCTGTTTCAAATCGTTTTC+TGG + chr4.3:43372311-43372330 None:intergenic 35.0%
! TTCTAACTTCACGAGTTTTG+AGG - chr4.3:43372250-43372269 MS.gene09153:intron 35.0%
! TTTCTTAAGATCGATGGACT+AGG - chr4.3:43373975-43373994 MS.gene09153:CDS 35.0%
!! CAAGCTTTAAACTATGTCTG+AGG - chr4.3:43374533-43374552 MS.gene09153:CDS 35.0%
!! CTTTTGCTGTAACTTGTAAG+TGG + chr4.3:43374501-43374520 None:intergenic 35.0%
!! TGAGCATTGGAAAGATGATA+TGG - chr4.3:43373917-43373936 MS.gene09153:CDS 35.0%
!!! CGTCGTTTTTATTTATGCCA+AGG + chr4.3:43371727-43371746 None:intergenic 35.0%
!!! TTGTGTTTTGAGTTGAGAGA+AGG + chr4.3:43371675-43371694 None:intergenic 35.0%
AAACTTTAGACAATGAGGGG+TGG - chr4.3:43373698-43373717 MS.gene09153:CDS 40.0%
AACATGGACGATGATTTAGC+AGG - chr4.3:43372428-43372447 MS.gene09153:intron 40.0%
AACTTTAGACAATGAGGGGT+GGG - chr4.3:43373699-43373718 MS.gene09153:CDS 40.0%
ACATGGACGATGATTTAGCA+GGG - chr4.3:43372429-43372448 MS.gene09153:intron 40.0%
ACATTCACGAATTCGGACAT+CGG - chr4.3:43372810-43372829 MS.gene09153:intron 40.0%
ACGACAGCATCTTGTATTTC+AGG + chr4.3:43374120-43374139 None:intergenic 40.0%
AGATGTTTGGATTGTTTGGG+AGG + chr4.3:43371568-43371587 None:intergenic 40.0%
AGCGGCGTTAATGATTAACA+TGG - chr4.3:43372412-43372431 MS.gene09153:intron 40.0%
AGGAAACTGGATGTTATTGG+TGG + chr4.3:43371765-43371784 None:intergenic 40.0%
ATAACCAACTGGTAGCAGTA+TGG + chr4.3:43372036-43372055 None:intergenic 40.0%
CACCATTCAAAAGATTGCGA+AGG - chr4.3:43374653-43374672 MS.gene09153:CDS 40.0%
CCTGCAATTATCATCAACTC+AGG - chr4.3:43371947-43371966 MS.gene09153:intron 40.0%
CGACAGCATCTTGTATTTCA+GGG + chr4.3:43374119-43374138 None:intergenic 40.0%
CTAGGAAACATACAGGAAAC+TGG + chr4.3:43371778-43371797 None:intergenic 40.0%
GAAATCATGTTGTGGTTACG+AGG - chr4.3:43373997-43374016 MS.gene09153:CDS 40.0%
GAACGAACAACATAGGCAAT+TGG + chr4.3:43374395-43374414 None:intergenic 40.0%
GATGTTTGGATTGTTTGGGA+GGG + chr4.3:43371567-43371586 None:intergenic 40.0%
GGAACTCTCCAAACAAATCA+AGG - chr4.3:43371853-43371872 MS.gene09153:CDS 40.0%
GTAATCTTGATCGGACAGTT+AGG - chr4.3:43372781-43372800 MS.gene09153:intron 40.0%
TCTATCGAAAATCCAAGCGT+AGG - chr4.3:43373058-43373077 MS.gene09153:CDS 40.0%
TGTGAAGAGGAATGAGACTA+AGG + chr4.3:43371591-43371610 None:intergenic 40.0%
TTATAACATACGCGCAGCTA+AGG - chr4.3:43372510-43372529 MS.gene09153:CDS 40.0%
TTCGCCAAAGCAAGAATCAT+CGG + chr4.3:43372632-43372651 None:intergenic 40.0%
TTGCCGATGTTTCATGTGTT+TGG - chr4.3:43372479-43372498 MS.gene09153:CDS 40.0%
TTTATCAAGATCAGTCGGTC+CGG - chr4.3:43372840-43372859 MS.gene09153:intron 40.0%
! ACCCTTCGCAATCTTTTGAA+TGG + chr4.3:43374658-43374677 None:intergenic 40.0%
! AGAAGATCTTCAAGTTCTGC+GGG + chr4.3:43374087-43374106 None:intergenic 40.0%
! CTATAGATTTCCTGATGACG+AGG - chr4.3:43374358-43374377 MS.gene09153:CDS 40.0%
! GTTTCATGTGTTTGGATTGG+CGG - chr4.3:43372487-43372506 MS.gene09153:CDS 40.0%
! TGAGTTAGAGGGGTTTTTGA+AGG - chr4.3:43372565-43372584 MS.gene09153:CDS 40.0%
! TGGACTAGGAAATCATGTTG+TGG - chr4.3:43373989-43374008 MS.gene09153:CDS 40.0%
! TTTGAAACAGACCGATACAG+CGG - chr4.3:43372319-43372338 MS.gene09153:intron 40.0%
!! CCTGAGTTGATGATAATTGC+AGG + chr4.3:43371950-43371969 None:intergenic 40.0%
!! GCCGTTTTATCAAGATCAGT+CGG - chr4.3:43372835-43372854 MS.gene09153:intron 40.0%
!! GGTGTAAAGAGTATTTGCAG+TGG + chr4.3:43371828-43371847 None:intergenic 40.0%
!! TCATTGAAGAGTATTGGGTC+TGG - chr4.3:43372683-43372702 MS.gene09153:intron 40.0%
!! TTTGAGTTGAGAGAAGGAGA+GGG + chr4.3:43371669-43371688 None:intergenic 40.0%
!!! AAAGTTTGTTTTGTGGCCTC+TGG + chr4.3:43373685-43373704 None:intergenic 40.0%
!!! ATTTCCCCCAATTGTTTTGG+AGG + chr4.3:43373166-43373185 None:intergenic 40.0%
!!! TTTCCCCCAATTGTTTTGGA+GGG + chr4.3:43373165-43373184 None:intergenic 40.0%
!!! TTTTGAGTTGAGAGAAGGAG+AGG + chr4.3:43371670-43371689 None:intergenic 40.0%
AAGAAGAGACGCTTGTGAAG+AGG + chr4.3:43371604-43371623 None:intergenic 45.0%
ACACAAGAACTTCATCGCAG+CGG - chr4.3:43372394-43372413 MS.gene09153:intron 45.0%
ACTCCCTCCAAAACAATTGG+GGG - chr4.3:43373159-43373178 MS.gene09153:CDS 45.0%
ACTGGATGTTATTGGTGGAG+AGG + chr4.3:43371760-43371779 None:intergenic 45.0%
AGCGTAGGTATTCGTCATTC+TGG - chr4.3:43373073-43373092 MS.gene09153:CDS 45.0%
AGGGACCTGTATATTCCATC+TGG + chr4.3:43371530-43371549 None:intergenic 45.0%
AGTGTTCCTAAGACAGCTTC+AGG - chr4.3:43374691-43374710 MS.gene09153:CDS 45.0%
CACTCCCTCCAAAACAATTG+GGG - chr4.3:43373158-43373177 MS.gene09153:CDS 45.0%
CATGGACGATGATTTAGCAG+GGG - chr4.3:43372430-43372449 MS.gene09153:intron 45.0%
CCACTCCCTCCAAAACAATT+GGG - chr4.3:43373157-43373176 MS.gene09153:CDS 45.0%
CGAAGCTCAAATAGTGAGTG+TGG - chr4.3:43373123-43373142 MS.gene09153:CDS 45.0%
CTGTACTCATCAGGAACTAC+AGG - chr4.3:43372347-43372366 MS.gene09153:intron 45.0%
GAAGTACGGTGGTTTCAATG+GGG - chr4.3:43372537-43372556 MS.gene09153:CDS 45.0%
GAATGACGAATACCTACGCT+TGG + chr4.3:43373073-43373092 None:intergenic 45.0%
GCACACAGTTTACTCAACCT+TGG - chr4.3:43371707-43371726 MS.gene09153:intron 45.0%
GGAGCACACATTCACGAATT+CGG - chr4.3:43372803-43372822 MS.gene09153:intron 45.0%
GTTGGGTGAACGAACAACAT+AGG + chr4.3:43374402-43374421 None:intergenic 45.0%
TCCATCAATTCCTTCCCAAG+AGG + chr4.3:43372716-43372735 None:intergenic 45.0%
TCCTCTTGGGAAGGAATTGA+TGG - chr4.3:43372712-43372731 MS.gene09153:intron 45.0%
TCTTGGGAAGGAATTGATGG+AGG - chr4.3:43372715-43372734 MS.gene09153:intron 45.0%
TGGGTACATACAGGAGATCT+TGG - chr4.3:43373718-43373737 MS.gene09153:CDS 45.0%
TGTTATTGGTGGAGAGGATG+AGG + chr4.3:43371754-43371773 None:intergenic 45.0%
TTCACCATACTGCTACCAGT+TGG - chr4.3:43372029-43372048 MS.gene09153:intron 45.0%
TTGAGAGAAGGAGAGGGTTT+GGG + chr4.3:43371663-43371682 None:intergenic 45.0%
TTGTTTGGAGAGTTCCGCAA+CGG + chr4.3:43371849-43371868 None:intergenic 45.0%
! AATGGGGAGGTTTGAGTTAG+AGG - chr4.3:43372553-43372572 MS.gene09153:CDS 45.0%
! AGGAAGTACGGTGGTTTCAA+TGG - chr4.3:43372535-43372554 MS.gene09153:CDS 45.0%
! ATGGGGAGGTTTGAGTTAGA+GGG - chr4.3:43372554-43372573 MS.gene09153:CDS 45.0%
! GGAAGTACGGTGGTTTCAAT+GGG - chr4.3:43372536-43372555 MS.gene09153:CDS 45.0%
!! TCAGCTGGAATGTTGGTTTC+AGG - chr4.3:43373097-43373116 MS.gene09153:CDS 45.0%
AATGAGGGGTGGGTACATAC+AGG - chr4.3:43373709-43373728 MS.gene09153:CDS 50.0%
AGTGGTGACTATTGCACCGA+TGG + chr4.3:43371810-43371829 None:intergenic 50.0%
GAGGGCTAGAGAAGGTCTTA+GGG + chr4.3:43371549-43371568 None:intergenic 50.0%
GCAGCTAAGGAGAGGAAGTA+CGG - chr4.3:43372523-43372542 MS.gene09153:CDS 50.0%
GCCAAAGCAAGAATCATCGG+TGG + chr4.3:43372629-43372648 None:intergenic 50.0%
GCCACCGATGATTCTTGCTT+TGG - chr4.3:43372625-43372644 MS.gene09153:intron 50.0%
GCCACTCCCTCCAAAACAAT+TGG - chr4.3:43373156-43373175 MS.gene09153:CDS 50.0%
GGAACTACAGGAGTGAGCAA+AGG - chr4.3:43372359-43372378 MS.gene09153:intron 50.0%
GTGGCAGCTAGGAAACATAC+AGG + chr4.3:43371785-43371804 None:intergenic 50.0%
GTTGAGAGAAGGAGAGGGTT+TGG + chr4.3:43371664-43371683 None:intergenic 50.0%
TCCTCGGACAGCAAACTGTT+GGG + chr4.3:43374419-43374438 None:intergenic 50.0%
TGCTTTGGCGAAACAGAGTG+TGG - chr4.3:43372640-43372659 MS.gene09153:intron 50.0%
TGGCGAAACAGAGTGTGGTT+GGG - chr4.3:43372645-43372664 MS.gene09153:intron 50.0%
TTCCTCGGACAGCAAACTGT+TGG + chr4.3:43374420-43374439 None:intergenic 50.0%
TTGGCGAAACAGAGTGTGGT+TGG - chr4.3:43372644-43372663 MS.gene09153:intron 50.0%
TTGGGGGAAATATGCGTACG+AGG - chr4.3:43373175-43373194 MS.gene09153:CDS 50.0%
TTGTTTGGGAGGGCTAGAGA+AGG + chr4.3:43371557-43371576 None:intergenic 50.0%
! AACCTATTTGAGGCACCAGC+TGG - chr4.3:43372958-43372977 MS.gene09153:intron 50.0%
! ACCTATTTGAGGCACCAGCT+GGG - chr4.3:43372959-43372978 MS.gene09153:intron 50.0%
! AGATTTCCTGATGACGAGGC+TGG - chr4.3:43374362-43374381 MS.gene09153:CDS 50.0%
! GTCGATGAGGGCGATTTTGT+CGG + chr4.3:43371630-43371649 None:intergenic 50.0%
! TCGATGAGGGCGATTTTGTC+GGG + chr4.3:43371629-43371648 None:intergenic 50.0%
! TGGGGAGGTTTGAGTTAGAG+GGG - chr4.3:43372555-43372574 MS.gene09153:CDS 50.0%
!! ATTCGTCATTCTGGCTCAGC+TGG - chr4.3:43373082-43373101 MS.gene09153:CDS 50.0%
!! GAGGCGTTGATTGAATCAGC+AGG - chr4.3:43372269-43372288 MS.gene09153:intron 50.0%
!! GTACAGAAGTGCCGCTGTAT+CGG + chr4.3:43372333-43372352 None:intergenic 50.0%
!! TTCTGGCTCAGCTGGAATGT+TGG - chr4.3:43373090-43373109 MS.gene09153:CDS 50.0%
!!! CCCAATTGTTTTGGAGGGAG+TGG + chr4.3:43373160-43373179 None:intergenic 50.0%
ACATACGCGCAGCTAAGGAG+AGG - chr4.3:43372515-43372534 MS.gene09153:CDS 55.0%
CGAGGTCCCAACATGATGCA+AGG - chr4.3:43373193-43373212 MS.gene09153:CDS 55.0%
GCGGCACTTCTGTACTCATC+AGG - chr4.3:43372338-43372357 MS.gene09153:intron 55.0%
GGAGGGCTAGAGAAGGTCTT+AGG + chr4.3:43371550-43371569 None:intergenic 55.0%
GTACGGTGGTTTCAATGGGG+AGG - chr4.3:43372540-43372559 MS.gene09153:CDS 55.0%
TCCCAGCTGGTGCCTCAAAT+AGG + chr4.3:43372963-43372982 None:intergenic 55.0%
TTCCTAGCTGCCACCTCCAT+CGG - chr4.3:43371791-43371810 MS.gene09153:intron 55.0%
! CGATGAGGGCGATTTTGTCG+GGG + chr4.3:43371628-43371647 None:intergenic 55.0%
! GACTATTGCACCGATGGAGG+TGG + chr4.3:43371804-43371823 None:intergenic 55.0%
! GATGAGGGCGATTTTGTCGG+GGG + chr4.3:43371627-43371646 None:intergenic 55.0%
! GCTAAGGAGAGGAAGTACGG+TGG - chr4.3:43372526-43372545 MS.gene09153:CDS 55.0%
! GGAAGAGTCTGCGTCGATGA+GGG + chr4.3:43371642-43371661 None:intergenic 55.0%
! GGTGACTATTGCACCGATGG+AGG + chr4.3:43371807-43371826 None:intergenic 55.0%
!! ACCCAACAGTTTGCTGTCCG+AGG - chr4.3:43374415-43374434 MS.gene09153:CDS 55.0%
ACCTCACCAGCCTCGTCATC+AGG + chr4.3:43374371-43374390 None:intergenic 60.0%
! GGGAAGAGTCTGCGTCGATG+AGG + chr4.3:43371643-43371662 None:intergenic 60.0%
! TCCTGATGACGAGGCTGGTG+AGG - chr4.3:43374367-43374386 MS.gene09153:CDS 60.0%
!! GGTCTGGTGCTGCTCCTCTT+GGG - chr4.3:43372699-43372718 MS.gene09153:intron 60.0%
!! TGGTGCTGCTCCTCTTGGGA+AGG - chr4.3:43372703-43372722 MS.gene09153:intron 60.0%
CACCGATGGAGGTGGCAGCT+AGG + chr4.3:43371796-43371815 None:intergenic 65.0%
!! GGGTCTGGTGCTGCTCCTCT+TGG - chr4.3:43372698-43372717 MS.gene09153:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 43371506 43374762 43371506 ID=MS.gene09153
chr4.3 mRNA 43371506 43374762 43371506 ID=MS.gene09153.t1;Parent=MS.gene09153
chr4.3 exon 43374300 43374762 43374300 ID=MS.gene09153.t1.exon1;Parent=MS.gene09153.t1
chr4.3 CDS 43374300 43374762 43374300 ID=cds.MS.gene09153.t1;Parent=MS.gene09153.t1
chr4.3 exon 43373493 43374037 43373493 ID=MS.gene09153.t1.exon2;Parent=MS.gene09153.t1
chr4.3 CDS 43373493 43374037 43373493 ID=cds.MS.gene09153.t1;Parent=MS.gene09153.t1
chr4.3 exon 43373054 43373243 43373054 ID=MS.gene09153.t1.exon3;Parent=MS.gene09153.t1
chr4.3 CDS 43373054 43373243 43373054 ID=cds.MS.gene09153.t1;Parent=MS.gene09153.t1
chr4.3 exon 43372455 43372600 43372455 ID=MS.gene09153.t1.exon4;Parent=MS.gene09153.t1
chr4.3 CDS 43372455 43372600 43372455 ID=cds.MS.gene09153.t1;Parent=MS.gene09153.t1
chr4.3 exon 43372123 43372190 43372123 ID=MS.gene09153.t1.exon5;Parent=MS.gene09153.t1
chr4.3 CDS 43372123 43372190 43372123 ID=cds.MS.gene09153.t1;Parent=MS.gene09153.t1
chr4.3 exon 43371802 43371904 43371802 ID=MS.gene09153.t1.exon6;Parent=MS.gene09153.t1
chr4.3 CDS 43371802 43371904 43371802 ID=cds.MS.gene09153.t1;Parent=MS.gene09153.t1
chr4.3 exon 43371506 43371619 43371506 ID=MS.gene09153.t1.exon7;Parent=MS.gene09153.t1
chr4.3 CDS 43371506 43371619 43371506 ID=cds.MS.gene09153.t1;Parent=MS.gene09153.t1
Gene Sequence

>MS.gene09153

ATGCAGAAATCTGGTTATAGTCCAGATGGAATATACAGGTCCCTAAGACCTTCTCTAGCCCTCCCAAACAATCCAAACATCTCCTTAGTCTCATTCCTCTTCACAAGCGTCTCTTCTTCCCCCGACAAAATCGCCCTCATCGACGCAGACTCTTCCCAAACCCTCTCCTTCTCTCAACTCAAAACACAAGTTGTAAAACTTGCACACAGTTTACTCAACCTTGGCATAAATAAAAACGACGTTGTCCTCATCCTCTCCACCAATAACATCCAGTTTCCTGTATGTTTCCTAGCTGCCACCTCCATCGGTGCAATAGTCACCACTGCAAATACTCTTTACACCGTTGCGGAACTCTCCAAACAAATCAAGGATTCCAATCCTAAATTAGTTATCACTGTACCTGAATTATGGAGCAAAGTGAAGAGTTTCAATTTTAATTTTCCTGCAATTATCATCAACTCAGGTAATATTTGATACAAAGTTAGTTATAGTTACTAGTTAGACTAGTTAGTTTTTGTCATCATTCACCATACTGCTACCAGTTGGTTATAGTTAGACTAATTAGTTGTTTTCTTCATTGGCTATATATTTGTTGCATCAGTTAGTTATAGTTAGACTAATTAGTTGTTTTCTTCATTGGCTATATATTTGTTGCATTGGTTAGTTTAGTTACACTAATTAGCTGTTCTCATCATTCTCTATATAACAAACTTTATATCTAATAGGTAATGCTACGGGTTTAAATTCTAACTTCACGAGTTTTGAGGCGTTGATTGAATCAGCAGGAGAAGTAACAGAGTTACCAGAAAACGATTTGAAACAGACCGATACAGCGGCACTTCTGTACTCATCAGGAACTACAGGAGTGAGCAAAGGAGTGATTCTCACACACAAGAACTTCATCGCAGCGGCGTTAATGATTAACATGGACGATGATTTAGCAGGGGAATTAAACAACGTGTTCATCTGCGTTTTGCCGATGTTTCATGTGTTTGGATTGGCGGTTATAACATACGCGCAGCTAAGGAGAGGAAGTACGGTGGTTTCAATGGGGAGGTTTGAGTTAGAGGGGTTTTTGAAGGTGATTGAGAAGTATAGAGTGACAAATTTGTGGGTTGTGCCACCGATGATTCTTGCTTTGGCGAAACAGAGTGTGGTTGGGAAATATGATGTTTCTTCATTGAAGAGTATTGGGTCTGGTGCTGCTCCTCTTGGGAAGGAATTGATGGAGGAGTGTGCTCGACGTCTACCTCATGTTACTATTTATCAGGTAAGGTAATCTTGATCGGACAGTTAGGGAGCACACATTCACGAATTCGGACATCGGTAGCCGTTTTATCAAGATCAGTCGGTCCGGATTAAAGATAGCGATTTTAAGTTATAAAACTGAAATTAAAGTATCTTGATCATTGGTAATTTTTTTACCAATGATCTGAATGTGTCATTGCATTGAACCTATTTGAGGCACCAGCTGGGATAAAAATGATTTATTTATTAATTGATTATCTTGTACTATTAGGGGTATGGTATGACAGAAACTTGTGCCGTTGTTTCTATCGAAAATCCAAGCGTAGGTATTCGTCATTCTGGCTCAGCTGGAATGTTGGTTTCAGGAGTCGAAGCTCAAATAGTGAGTGTGGATACACAGAAGCCACTCCCTCCAAAACAATTGGGGGAAATATGCGTACGAGGTCCCAACATGATGCAAGGTAAATTGTCAGCACATTCAACATTTACACTCCTACAAATAACTCTAAGTGATTTTCCAACAGAATACAATTATATTGTACATGTAGTGTTCTTGTTATGCTTTAAGGAATTCAGTAAAAAAAATGACTTGCATTGTTGTATATGCTACTTTCTGATATACATCTATTTTTTCTATTAAAAAATTCAACTTCTTAGTGTATTTGTAAATTCTCTACCAATGAAACTTCTCTTGATCAACTAATAATTAGAAACATGAACTTTAAGTTCCTTTTCATGCTTTTAAATAACATTGTAATCATAATGCATTTTATGATCATACTTACTTGTACAAAGTCTCTGTAGAGTACAATGGCTAGCACTGTTTCTCCAAGTCTTGCAGTTTTTATATCTCCAACTTTATGATTGGAATGTAAATTTTTCCCATATGAAACTTTTCCTATACAATATTTCAGGTTATTATAACAATCCAGAGGCCACAAAACAAACTTTAGACAATGAGGGGTGGGTACATACAGGAGATCTTGGATATTTTGATGAGATCGGCCAACTTTATGTTGTTGATCGTATCAAAGAACTGATCAAATACAAAGGTTATCAGGTATGTAATCTAAAATGATATCGATTGTAGTAACTAAAGTAACATATTTCATCTATTCATTATCTGAAATCTTAGCCAAATCCTTGTCACTGTTATGTTTACTTGAGCATTGGAAAGATGATATGGTATTGGTTGTATTAATCAAATGATCCTCATTAATATTTCTTAAGATCGATGGACTAGGAAATCATGTTGTGGTTACGAGGATTATTTTTGACAAGGAATTTCACATTATGTAATATTTGTCTCTTTGAAATTAACTAGGTTGCGCCCGCAGAACTTGAAGATCTTCTTGTTTCTCACCCTGAAATACAAGATGCTGTCGTTATCCCGTGAGTAAAACTTGCATGATAGTTTACGTTTGAGGGATGTTACTTACATCAAAAAATTGAAAAATATCCTAACTTGTAACAATTGGTTTGACACAGAGAAAAAAGTAATAAAAAATGAAATCGTACACCATATAAATATACTGTGATGGACAACAAAACTGTTTTCCAATTGATATTTCTCTATTAATAATTCACATTTGAGTTACTTATTGCTATAGATTTCCTGATGACGAGGCTGGTGAGGTTCCAATTGCCTATGTTGTTCGTTCACCCAACAGTTTGCTGTCCGAGGAAGAAATTCAGAAGTTTATTGCTAATCAGGTGAGAAAAAGCTAAGTAACAAGTTTTATCTCCACTTACAAGTTACAGCAAAAGAAACACATTTTGCAAGCTTTAAACTATGTCTGAGGAATGAAACTGTTTTTAACCCTGTATTTATGTTTTTAATGGTGGTCAACTTATTTCATTTGCCAATTTCAACTAATTTTTAAACACGCTTATAGGTTGCACCATTCAAAAGATTGCGAAGGGTTACATTCATCAACAGTGTTCCTAAGACAGCTTCAGGAAAAATTCTCAGAAGAGAGCTTATTGCAAAAGCACGATCCAAAATATAA
Protein sequence

>MS.gene09153.t1

MQKSGYSPDGIYRSLRPSLALPNNPNISLVSFLFTSVSSSPDKIALIDADSSQTLSFSQLKTQVVKLAHSLLNLGINKNDVVLILSTNNIQFPVCFLAATSIGAIVTTANTLYTVAELSKQIKDSNPKLVITVPELWSKVKSFNFNFPAIIINSGNATGLNSNFTSFEALIESAGEVTELPENDLKQTDTAALLYSSGTTGVSKGVILTHKNFIAAALMINMDDDLAGELNNVFICVLPMFHVFGLAVITYAQLRRGSTVVSMGRFELEGFLKVIEKYRVTNLWVVPPMILALAKQSVVGKYDVSSLKSIGSGAAPLGKELMEECARRLPHVTIYQGYGMTETCAVVSIENPSVGIRHSGSAGMLVSGVEAQIVSVDTQKPLPPKQLGEICVRGPNMMQGYYNNPEATKQTLDNEGWVHTGDLGYFDEIGQLYVVDRIKELIKYKGYQVAPAELEDLLVSHPEIQDAVVIPFPDDEAGEVPIAYVVRSPNSLLSEEEIQKFIANQVAPFKRLRRVTFINSVPKTASGKILRRELIAKARSKI