AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007715

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007715.t1 MTR_4g057730 94.649 542 29 0 1 542 1 542 0.0 1053
MS.gene007715.t1 MTR_4g057730 94.692 471 25 0 1 471 1 471 0.0 920
MS.gene007715.t1 MTR_4g057730 94.444 450 25 0 1 450 1 450 0.0 877
MS.gene007715.t1 MTR_2g095510 72.509 542 146 2 1 542 1 539 0.0 820
MS.gene007715.t1 MTR_2g095510 68.889 450 137 2 1 450 1 447 0.0 652
MS.gene007715.t1 MTR_1g063110 58.240 534 216 6 12 539 13 545 0.0 612
MS.gene007715.t1 MTR_1g063120 57.565 542 211 6 12 539 13 549 0.0 605
MS.gene007715.t1 MTR_1g026310 45.338 547 289 5 3 542 17 560 1.86e-157 461
MS.gene007715.t1 MTR_1g090737 42.077 549 303 7 3 542 24 566 8.82e-149 439
MS.gene007715.t1 MTR_8g039720 45.290 552 282 8 4 542 15 559 3.93e-148 437
MS.gene007715.t1 MTR_2g105570 41.791 536 300 8 11 537 12 544 9.31e-143 423
MS.gene007715.t1 MTR_5g007640 43.094 543 284 8 11 540 55 585 8.38e-142 422
MS.gene007715.t1 MTR_5g027480 41.481 540 301 7 2 534 16 547 1.66e-138 412
MS.gene007715.t1 MTR_4g128337 42.185 531 299 5 11 537 17 543 3.88e-138 411
MS.gene007715.t1 MTR_4g005750 41.199 534 300 8 11 537 11 537 7.14e-134 400
MS.gene007715.t1 MTR_3g088870 42.495 513 275 6 30 535 52 551 1.58e-132 397
MS.gene007715.t1 MTR_1g073180 43.295 522 265 10 26 537 29 529 3.83e-129 388
MS.gene007715.t1 MTR_5g027500 40.395 557 303 11 6 542 21 568 1.57e-128 387
MS.gene007715.t1 MTR_3g088880 41.829 514 274 9 30 535 53 549 2.52e-128 386
MS.gene007715.t1 MTR_4g077900 41.079 482 274 6 67 542 1 478 2.12e-123 371
MS.gene007715.t1 MTR_3g460770 35.417 528 327 7 21 542 22 541 7.56e-120 364
MS.gene007715.t1 MTR_1g073180 42.032 502 260 10 26 517 51 531 3.56e-115 352
MS.gene007715.t1 MTR_1g026470 39.642 391 226 5 3 386 17 404 5.90e-82 261
MS.gene007715.t1 MTR_1g026430 39.691 388 224 5 3 383 17 401 2.64e-81 260
MS.gene007715.t1 MTR_1569s0010 29.887 532 333 9 35 534 4 527 1.30e-58 203
MS.gene007715.t1 MTR_3g035130 33.061 490 300 15 55 532 32 505 1.60e-57 200
MS.gene007715.t1 MTR_0803s0020 28.281 541 349 14 7 528 214 734 6.31e-49 182
MS.gene007715.t1 MTR_3g064420 26.606 545 357 10 30 540 17 552 5.71e-47 173
MS.gene007715.t1 MTR_3g035130 32.952 437 265 15 55 479 32 452 2.65e-46 169
MS.gene007715.t1 MTR_5g008820 27.290 535 350 14 30 540 17 536 9.44e-45 166
MS.gene007715.t1 MTR_2g044180 27.289 557 331 20 32 539 25 556 8.46e-43 161
MS.gene007715.t1 MTR_2g044180 31.888 392 216 16 181 539 92 465 4.01e-40 152
MS.gene007715.t1 MTR_4g057755 87.671 73 9 0 398 470 46 118 4.27e-39 139
MS.gene007715.t1 MTR_4g057755 76.667 60 13 1 222 281 1 59 4.35e-23 95.1
MS.gene007715.t1 MTR_4g011790 25.954 524 339 15 47 534 81 591 4.33e-39 151
MS.gene007715.t1 MTR_5g008800 26.335 543 347 13 30 540 17 538 4.65e-39 150
MS.gene007715.t1 MTR_4g013210 29.867 375 228 10 189 534 70 438 6.64e-39 148
MS.gene007715.t1 MTR_4g121600 26.381 561 349 21 23 539 16 556 1.92e-38 149
MS.gene007715.t1 MTR_5g076220 26.937 568 335 16 23 540 10 547 3.74e-38 148
MS.gene007715.t1 MTR_4g011790 25.856 526 341 15 47 536 81 593 1.31e-37 147
MS.gene007715.t1 MTR_4g011790 25.328 533 340 16 47 534 81 600 4.71e-37 145
MS.gene007715.t1 MTR_8g074040 24.408 549 366 14 23 540 52 582 1.92e-35 140
MS.gene007715.t1 MTR_5g076240 25.314 557 353 16 15 531 4 537 2.29e-35 140
MS.gene007715.t1 MTR_4g013210 28.375 363 208 11 189 534 70 397 6.14e-34 133
MS.gene007715.t1 MTR_4g013210 28.375 363 208 11 189 534 222 549 3.15e-33 133
MS.gene007715.t1 MTR_5g076200 25.586 555 357 16 23 539 10 546 3.78e-33 133
MS.gene007715.t1 MTR_2g030240 25.143 525 343 12 59 542 32 547 3.96e-33 133
MS.gene007715.t1 MTR_4g013210 29.630 351 212 10 189 510 222 566 5.82e-33 133
MS.gene007715.t1 MTR_8g074120 26.203 374 241 9 187 540 135 493 1.52e-31 128
MS.gene007715.t1 MTR_7g084850 24.715 526 340 13 54 534 202 716 1.59e-31 129
MS.gene007715.t1 MTR_0161s0050 24.955 557 360 14 23 539 10 548 1.95e-30 125
MS.gene007715.t1 MTR_1g104810 30.514 331 155 14 186 459 256 568 4.57e-29 122
MS.gene007715.t1 MTR_1g104810 30.514 331 155 14 186 459 189 501 5.84e-29 121
MS.gene007715.t1 MTR_8g074040 23.881 469 310 13 103 540 12 464 4.55e-28 117
MS.gene007715.t1 MTR_5g023730 24.432 528 360 17 30 531 19 533 1.22e-26 114
MS.gene007715.t1 MTR_2g030240 26.166 386 240 10 193 542 77 453 3.06e-26 112
MS.gene007715.t1 MTR_1g034360 24.125 514 290 19 32 464 49 543 4.31e-25 110
MS.gene007715.t1 MTR_5g009360 28.375 363 190 14 163 469 75 423 6.50e-23 102
MS.gene007715.t1 MTR_1g026350 43.284 134 73 2 3 135 17 148 1.13e-22 95.5
MS.gene007715.t1 MTR_5g009360 25.820 488 270 19 56 469 80 549 1.26e-22 102
MS.gene007715.t1 MTR_7g109830 27.390 387 202 17 189 506 221 597 1.27e-22 102
MS.gene007715.t1 MTR_1g086600 25.389 386 213 12 189 506 243 621 2.13e-22 101
MS.gene007715.t1 MTR_4g068670 23.063 542 304 21 6 464 30 541 1.36e-21 99.4
MS.gene007715.t1 MTR_5g009350 26.608 342 183 12 186 470 220 550 5.34e-21 97.1
MS.gene007715.t1 MTR_1054s0020 23.674 528 366 15 13 529 258 759 5.94e-21 97.4
MS.gene007715.t1 MTR_5g009350 27.353 340 181 12 186 469 220 549 9.84e-21 96.7
MS.gene007715.t1 MTR_5g009350 27.353 340 181 12 186 469 220 549 1.15e-20 96.3
MS.gene007715.t1 MTR_5g009350 26.393 341 183 12 186 469 220 549 1.23e-20 95.9
MS.gene007715.t1 MTR_3g084470 30.328 244 135 6 332 542 469 710 1.74e-20 95.9
MS.gene007715.t1 MTR_3g084470 25.738 237 150 7 35 248 83 316 1.08e-13 74.3
MS.gene007715.t1 MTR_8g083170 36.842 171 91 4 311 470 459 623 1.79e-20 95.9
MS.gene007715.t1 MTR_4g013210 26.543 324 182 11 189 505 222 496 2.91e-20 94.4
MS.gene007715.t1 MTR_3g084450 28.333 240 136 5 337 542 270 507 9.43e-19 89.7
MS.gene007715.t1 MTR_3g084450 27.755 245 141 5 332 542 473 715 1.62e-18 89.7
MS.gene007715.t1 MTR_4g013210 28.992 238 145 7 189 408 222 453 5.99e-16 80.9
MS.gene007715.t1 MTR_8g099420 33.898 177 102 7 289 460 375 541 2.04e-15 79.7
MS.gene007715.t1 MTR_3g088890 45.312 64 35 0 444 507 10 73 2.62e-15 72.0
MS.gene007715.t1 MTR_1g030290 31.776 214 110 12 312 509 426 619 7.48e-14 74.7
MS.gene007715.t1 MTR_2g098490 24.819 415 240 17 182 537 640 1041 2.23e-13 73.9
MS.gene007715.t1 MTR_1586s0010 21.096 365 238 7 66 399 81 426 3.62e-13 72.0
MS.gene007715.t1 MTR_3g087980 27.559 254 136 12 296 517 412 649 1.38e-11 67.8
MS.gene007715.t1 MTR_4g066130 22.922 397 255 15 189 537 651 1044 6.37e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007715.t1 AT4G05160 66.850 546 175 2 1 542 1 544 0.0 760
MS.gene007715.t1 AT1G20510 44.946 554 271 8 3 540 7 542 1.04e-156 458
MS.gene007715.t1 AT1G20500 44.685 555 275 10 3 542 13 550 1.45e-147 435
MS.gene007715.t1 AT1G65060 43.097 536 286 8 11 537 34 559 4.76e-144 427
MS.gene007715.t1 AT3G21240 42.037 540 296 8 7 537 18 549 2.77e-143 424
MS.gene007715.t1 AT1G20480 42.703 555 292 10 2 542 23 565 5.51e-141 419
MS.gene007715.t1 AT5G38120 42.626 556 284 10 3 542 14 550 1.89e-139 414
MS.gene007715.t1 AT4G19010 41.360 544 287 11 7 534 27 554 4.70e-136 406
MS.gene007715.t1 AT5G63380 41.621 543 285 12 10 534 22 550 4.34e-135 404
MS.gene007715.t1 AT1G51680 40.951 547 307 7 2 537 15 556 1.38e-134 402
MS.gene007715.t1 AT1G20510 43.093 485 242 8 3 471 7 473 1.51e-124 374
MS.gene007715.t1 AT1G51680 40.310 516 292 7 2 506 15 525 3.21e-122 370
MS.gene007715.t1 AT5G38120 41.346 520 270 10 3 506 14 514 3.44e-122 369
MS.gene007715.t1 AT3G21230 38.828 546 313 8 7 537 24 563 2.12e-121 369
MS.gene007715.t1 AT3G21240 40.842 475 264 8 7 472 18 484 1.39e-116 354
MS.gene007715.t1 AT3G21240 40.842 475 264 8 7 472 18 484 1.39e-116 354
MS.gene007715.t1 AT1G62940 36.735 539 320 9 11 542 12 536 1.18e-115 353
MS.gene007715.t1 AT1G20510 41.469 463 237 8 3 449 7 451 4.56e-111 339
MS.gene007715.t1 AT3G21230 38.104 538 306 11 7 529 24 549 1.75e-109 337
MS.gene007715.t1 AT1G51680 40.000 480 272 7 2 470 15 489 5.38e-109 334
MS.gene007715.t1 AT1G65060 41.111 450 246 8 11 451 34 473 1.87e-108 333
MS.gene007715.t1 AT5G38120 40.412 485 254 10 3 471 14 479 7.76e-107 328
MS.gene007715.t1 AT5G38120 40.412 485 254 10 3 471 14 479 7.76e-107 328
MS.gene007715.t1 AT3G21230 37.996 479 276 8 7 470 24 496 7.97e-98 306
MS.gene007715.t1 AT1G20490 38.051 431 237 13 3 418 13 428 1.60e-82 264
MS.gene007715.t1 AT1G20490 38.051 431 237 13 3 418 13 428 1.60e-82 264
MS.gene007715.t1 AT3G48990 31.746 504 315 14 41 532 18 504 6.15e-59 204
MS.gene007715.t1 AT3G21230 33.741 409 250 7 7 400 24 426 8.75e-58 199
MS.gene007715.t1 AT1G65890 25.887 564 361 17 18 538 5 554 1.90e-47 174
MS.gene007715.t1 AT1G77240 27.207 555 357 15 19 540 3 543 1.18e-46 171
MS.gene007715.t1 AT1G65880 25.044 563 368 16 18 538 5 555 2.24e-45 168
MS.gene007715.t1 AT1G66120 26.316 551 366 13 19 538 6 547 4.03e-42 159
MS.gene007715.t1 AT1G21540 27.143 560 356 18 19 540 3 548 6.66e-42 158
MS.gene007715.t1 AT3G16170 25.859 553 334 17 34 534 72 600 1.70e-40 155
MS.gene007715.t1 AT1G30520 27.308 509 327 15 64 534 42 545 1.03e-39 152
MS.gene007715.t1 AT1G30520 27.308 509 327 15 64 534 42 545 1.03e-39 152
MS.gene007715.t1 AT1G68270 24.910 558 336 18 19 536 6 520 1.75e-36 142
MS.gene007715.t1 AT1G21530 25.448 558 367 20 19 540 3 547 9.49e-36 140
MS.gene007715.t1 AT1G21530 26.025 561 358 23 19 540 3 545 2.29e-35 139
MS.gene007715.t1 AT5G16340 25.397 567 360 20 15 541 4 547 2.46e-35 139
MS.gene007715.t1 AT5G16370 25.136 553 371 18 15 537 4 543 1.49e-34 137
MS.gene007715.t1 AT1G20560 24.182 550 354 16 30 540 19 544 2.60e-34 137
MS.gene007715.t1 AT1G76290 26.079 533 363 16 27 539 14 535 3.45e-34 136
MS.gene007715.t1 AT1G75960 24.505 555 371 15 15 537 4 542 3.52e-34 136
MS.gene007715.t1 AT5G27600 26.527 524 291 20 11 457 63 569 1.91e-32 132
MS.gene007715.t1 AT2G17650 24.000 575 367 19 12 538 45 597 5.30e-32 130
MS.gene007715.t1 AT3G16910 24.779 565 336 22 32 539 27 559 5.64e-32 130
MS.gene007715.t1 AT5G36880 24.621 528 338 16 54 534 147 661 8.46e-31 127
MS.gene007715.t1 AT5G36880 24.621 528 338 16 54 534 147 661 8.46e-31 127
MS.gene007715.t1 AT5G36880 24.621 528 338 16 54 534 147 661 8.46e-31 127
MS.gene007715.t1 AT5G36880 24.621 528 338 16 54 534 147 661 8.46e-31 127
MS.gene007715.t1 AT5G36880 24.621 528 338 16 54 534 197 711 1.12e-30 127
MS.gene007715.t1 AT1G30520 25.995 427 284 13 64 466 42 460 2.62e-30 124
MS.gene007715.t1 AT1G30520 25.995 427 284 13 64 466 42 460 2.62e-30 124
MS.gene007715.t1 AT1G20560 26.316 380 233 11 187 540 108 466 2.00e-29 121
MS.gene007715.t1 AT1G64400 25.287 522 305 23 11 457 27 538 2.09e-26 114
MS.gene007715.t1 AT3G05970 30.063 316 172 10 186 457 259 569 2.25e-24 107
MS.gene007715.t1 AT2G47240 26.402 428 237 17 179 535 209 629 1.57e-23 105
MS.gene007715.t1 AT2G47240 26.402 428 237 17 179 535 209 629 1.57e-23 105
MS.gene007715.t1 AT2G47240 26.402 428 237 17 179 535 209 629 1.57e-23 105
MS.gene007715.t1 AT2G47240 26.402 428 237 17 179 535 150 570 1.62e-23 105
MS.gene007715.t1 AT5G36880 23.861 461 294 15 54 470 147 594 4.05e-23 103
MS.gene007715.t1 AT1G49430 28.021 389 205 15 186 506 221 602 4.88e-23 103
MS.gene007715.t1 AT4G23850 22.674 516 310 17 9 457 45 538 2.93e-20 95.1
MS.gene007715.t1 AT4G11030 24.897 486 278 19 56 470 81 550 5.42e-19 91.3
MS.gene007715.t1 AT3G23790 28.904 301 171 10 279 542 423 717 2.69e-18 89.0
MS.gene007715.t1 AT4G14070 38.000 150 80 4 332 470 490 637 5.01e-18 88.2
MS.gene007715.t1 AT4G14070 23.932 234 155 8 35 247 105 336 5.30e-12 68.9
MS.gene007715.t1 AT1G77590 34.104 173 89 9 312 469 276 438 1.74e-13 73.6
MS.gene007715.t1 AT1G77590 34.104 173 89 9 312 469 422 584 2.74e-13 73.2
MS.gene007715.t1 AT5G35930 25.195 385 240 12 181 529 95 467 2.14e-11 67.4
MS.gene007715.t1 AT2G04350 33.766 154 80 7 334 473 472 617 2.23e-11 67.0
MS.gene007715.t1 AT2G04350 33.766 154 80 7 334 473 472 617 2.23e-11 67.0
MS.gene007715.t1 AT5G35930 25.195 385 240 12 181 529 187 559 2.66e-11 67.0

Find 134 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATTATATCTTATATTT+TGG 0.116812 4.4:+45661510 None:intergenic
GGTACAGTGATAACTAATTT+AGG 0.209914 4.4:+45664378 None:intergenic
GGAGGGCTAGAGAAGGTCTT+AGG 0.217215 4.4:+45664715 None:intergenic
AAGGAGATGTTTGGATTGTT+TGG 0.225138 4.4:+45664693 None:intergenic
TCAGCTGGAATGTTGGTTTC+AGG 0.248160 4.4:-45663165 MS.gene007715:CDS
TCAGAAGTTTATTGCTAATC+AGG 0.249995 4.4:-45661817 MS.gene007715:intron
ACGACAGCATCTTGTATTTC+AGG 0.265567 4.4:+45662145 None:intergenic
TTATCACTGTACCTGAATTA+TGG 0.271891 4.4:-45664368 MS.gene007715:CDS
GTTGAGAGAAGGAGAGGGTT+TGG 0.278616 4.4:+45664601 None:intergenic
TTCACTTTGCTCCATAATTC+AGG 0.281829 4.4:+45664357 None:intergenic
AATGGGGAGGTTTGAGTTAG+AGG 0.302369 4.4:-45663709 MS.gene007715:CDS
TTTCTTCATTGAAGAGTATT+GGG 0.309637 4.4:-45663584 MS.gene007715:CDS
ACCTCATGTTACTATTTATC+AGG 0.312959 4.4:-45663508 MS.gene007715:intron
ATTGGAATCCTTGATTTGTT+TGG 0.316951 4.4:+45664401 None:intergenic
TTGAGAGAAGGAGAGGGTTT+GGG 0.319270 4.4:+45664602 None:intergenic
GATCAAATACAAAGGTTATC+AGG 0.342076 4.4:-45662470 MS.gene007715:intron
TTGCCGATGTTTCATGTGTT+TGG 0.353705 4.4:-45663783 MS.gene007715:CDS
AGCGTAGGTATTCGTCATTC+TGG 0.360952 4.4:-45663189 MS.gene007715:CDS
GCCACTCCCTCCAAAACAAT+TGG 0.363709 4.4:-45663106 MS.gene007715:CDS
TACAGGAAACTGGATGTTAT+TGG 0.371299 4.4:+45664497 None:intergenic
GGGTCTGGTGCTGCTCCTCT+TGG 0.372270 4.4:-45663564 MS.gene007715:CDS
AATGAGACTAAGGAGATGTT+TGG 0.381371 4.4:+45664684 None:intergenic
AGGAGATGTTTGGATTGTTT+GGG 0.382762 4.4:+45664694 None:intergenic
TCTCTTTCAATGCAGAAATC+TGG 0.384408 4.4:-45664765 None:intergenic
CCTGAGTTGATGATAATTGC+AGG 0.391344 4.4:+45664315 None:intergenic
GCCACCGATGATTCTTGCTT+TGG 0.397468 4.4:-45663637 MS.gene007715:CDS
ACCTCACCAGCCTCGTCATC+AGG 0.398355 4.4:+45661894 None:intergenic
CCACTCCCTCCAAAACAATT+GGG 0.408832 4.4:-45663105 MS.gene007715:CDS
AGGAAGTACGGTGGTTTCAA+TGG 0.410372 4.4:-45663727 MS.gene007715:CDS
GTTTCTTCATTGAAGAGTAT+TGG 0.413086 4.4:-45663585 MS.gene007715:CDS
CTAGGAAACATACAGGAAAC+TGG 0.415218 4.4:+45664487 None:intergenic
TCCTCGGACAGCAAACTGTT+GGG 0.419851 4.4:+45661846 None:intergenic
TCCTCTTGGGAAGGAATTGA+TGG 0.427485 4.4:-45663550 MS.gene007715:CDS
AATTTACCTTGCATCATGTT+GGG 0.436629 4.4:+45663063 None:intergenic
ATTCGTCATTCTGGCTCAGC+TGG 0.439809 4.4:-45663180 MS.gene007715:CDS
TGGGTACATACAGGAGATCT+TGG 0.444644 4.4:-45662544 MS.gene007715:CDS
TTCCTCGGACAGCAAACTGT+TGG 0.447080 4.4:+45661845 None:intergenic
GGTCTGGTGCTGCTCCTCTT+GGG 0.450711 4.4:-45663563 MS.gene007715:CDS
TTCTGGCTCAGCTGGAATGT+TGG 0.451563 4.4:-45663172 MS.gene007715:CDS
GGAAGTACGGTGGTTTCAAT+GGG 0.453310 4.4:-45663726 MS.gene007715:CDS
AGTGTTCCTAAGACAGCTTC+AGG 0.454588 4.4:-45661571 MS.gene007715:CDS
AGTATAGAGTGACAAATTTG+TGG 0.464020 4.4:-45663665 MS.gene007715:CDS
GAACGAACAACATAGGCAAT+TGG 0.465645 4.4:+45661870 None:intergenic
CAATTTACCTTGCATCATGT+TGG 0.472409 4.4:+45663062 None:intergenic
GATGTTTCATGTGTTTGGAT+TGG 0.473581 4.4:-45663778 MS.gene007715:CDS
ATCTTGTACTATTAGGGGTA+TGG 0.475615 4.4:-45663252 MS.gene007715:intron
GTTTCATGTGTTTGGATTGG+CGG 0.478530 4.4:-45663775 MS.gene007715:CDS
CGACAGCATCTTGTATTTCA+GGG 0.482401 4.4:+45662146 None:intergenic
ATGGGGAGGTTTGAGTTAGA+GGG 0.485043 4.4:-45663708 MS.gene007715:CDS
TCATTGAAGAGTATTGGGTC+TGG 0.485714 4.4:-45663579 MS.gene007715:CDS
CTGTACTCATCAGGAACTAC+AGG 0.487926 4.4:-45663915 MS.gene007715:CDS
AACATGGACGATGATTTAGC+AGG 0.490835 4.4:-45663834 MS.gene007715:CDS
AGGGACCTGTATATTCCATC+TGG 0.491867 4.4:+45664735 None:intergenic
TGGCGAAACAGAGTGTGGTT+GGG 0.494845 4.4:-45663617 MS.gene007715:CDS
TTATAACATACGCGCAGCTA+AGG 0.495514 4.4:-45663752 MS.gene007715:CDS
TTCCTAGCTGCCACCTCCAT+CGG 0.495897 4.4:-45664471 MS.gene007715:CDS
AGTGATAACTAATTTAGGAT+TGG 0.497297 4.4:+45664383 None:intergenic
GCGGCACTTCTGTACTCATC+AGG 0.505987 4.4:-45663924 MS.gene007715:CDS
GTATAGAGTGACAAATTTGT+GGG 0.509344 4.4:-45663664 MS.gene007715:CDS
GAGGGCTAGAGAAGGTCTTA+GGG 0.511707 4.4:+45664716 None:intergenic
TACGATCAACAACATAAAGT+TGG 0.524847 4.4:+45662503 None:intergenic
CCTGCAATTATCATCAACTC+AGG 0.525372 4.4:-45664315 MS.gene007715:intron
TCCTGATGACGAGGCTGGTG+AGG 0.526657 4.4:-45661895 MS.gene007715:CDS
AGATTTCCTGATGACGAGGC+TGG 0.527482 4.4:-45661900 MS.gene007715:CDS
GCACACAGTTTACTCAACCT+TGG 0.529470 4.4:-45664555 MS.gene007715:CDS
AAACTTCTGAATTTCTTCCT+CGG 0.530812 4.4:+45661830 None:intergenic
TGGTGCTGCTCCTCTTGGGA+AGG 0.534764 4.4:-45663559 MS.gene007715:CDS
TTTGAGTTGAGAGAAGGAGA+GGG 0.536638 4.4:+45664596 None:intergenic
TATCTAATAGGTAATGCTAC+GGG 0.537453 4.4:-45664041 MS.gene007715:intron
GAATGACGAATACCTACGCT+TGG 0.539411 4.4:+45663192 None:intergenic
TGTTATTGGTGGAGAGGATG+AGG 0.539934 4.4:+45664511 None:intergenic
GGAACTCTCCAAACAAATCA+AGG 0.541312 4.4:-45664409 MS.gene007715:CDS
AATGAGGGGTGGGTACATAC+AGG 0.542233 4.4:-45662553 MS.gene007715:CDS
ATATCTAATAGGTAATGCTA+CGG 0.542243 4.4:-45664042 MS.gene007715:intron
GTACAGAAGTGCCGCTGTAT+CGG 0.543298 4.4:+45663932 None:intergenic
AGATGTTTGGATTGTTTGGG+AGG 0.543747 4.4:+45664697 None:intergenic
TGTGAAGAGGAATGAGACTA+AGG 0.543904 4.4:+45664674 None:intergenic
AAAACAAACTTTAGACAATG+AGG 0.546586 4.4:-45662569 MS.gene007715:CDS
AGAAGATCTTCAAGTTCTGC+GGG 0.547646 4.4:+45662178 None:intergenic
ATAGTCCAGATGGAATATAC+AGG 0.549923 4.4:-45664740 MS.gene007715:CDS
GATGTTTGGATTGTTTGGGA+GGG 0.551181 4.4:+45664698 None:intergenic
GAGGCGTTGATTGAATCAGC+AGG 0.553384 4.4:-45663993 MS.gene007715:CDS
TTGGCGAAACAGAGTGTGGT+TGG 0.557745 4.4:-45663618 MS.gene007715:CDS
AAATCTGGTTATAGTCCAGA+TGG 0.559141 4.4:-45664750 MS.gene007715:CDS
ACCCAACAGTTTGCTGTCCG+AGG 0.559615 4.4:-45661847 MS.gene007715:CDS
CACCATTCAAAAGATTGCGA+AGG 0.561043 4.4:-45661609 MS.gene007715:CDS
ACATGGACGATGATTTAGCA+GGG 0.561737 4.4:-45663833 MS.gene007715:CDS
GGAAGAGTCTGCGTCGATGA+GGG 0.562446 4.4:+45664623 None:intergenic
TGGGGAGGTTTGAGTTAGAG+GGG 0.562823 4.4:-45663707 MS.gene007715:CDS
AAACAAACTTTAGACAATGA+GGG 0.563086 4.4:-45662568 MS.gene007715:CDS
GCAGCTAAGGAGAGGAAGTA+CGG 0.565977 4.4:-45663739 MS.gene007715:CDS
ACCATTCAAAAGATTGCGAA+GGG 0.569226 4.4:-45661608 MS.gene007715:CDS
AACTTTAGACAATGAGGGGT+GGG 0.570046 4.4:-45662563 MS.gene007715:CDS
CACCGATGGAGGTGGCAGCT+AGG 0.571967 4.4:+45664469 None:intergenic
GGGAAGAGTCTGCGTCGATG+AGG 0.577257 4.4:+45664622 None:intergenic
CACTCCCTCCAAAACAATTG+GGG 0.578348 4.4:-45663104 MS.gene007715:CDS
CGAAGCTCAAATAGTGAGTG+TGG 0.579940 4.4:-45663139 MS.gene007715:CDS
AGGAAACTGGATGTTATTGG+TGG 0.583013 4.4:+45664500 None:intergenic
GTTGGGTGAACGAACAACAT+AGG 0.583728 4.4:+45661863 None:intergenic
GTGGCAGCTAGGAAACATAC+AGG 0.584515 4.4:+45664480 None:intergenic
CTATAGATTTCCTGATGACG+AGG 0.586638 4.4:-45661904 MS.gene007715:intron
GACTATTGCACCGATGGAGG+TGG 0.596107 4.4:+45664461 None:intergenic
AAGAAGATCTTCAAGTTCTG+CGG 0.597098 4.4:+45662177 None:intergenic
AAGAAGAGACGCTTGTGAAG+AGG 0.603096 4.4:+45664661 None:intergenic
TTCGCCAAAGCAAGAATCAT+CGG 0.605055 4.4:+45663633 None:intergenic
TTGTTTGGAGAGTTCCGCAA+CGG 0.605682 4.4:+45664416 None:intergenic
GGAACTACAGGAGTGAGCAA+AGG 0.615795 4.4:-45663903 MS.gene007715:CDS
AAATACTCTTTACACCGTTG+CGG 0.620177 4.4:-45664430 MS.gene007715:CDS
TCTATCGAAAATCCAAGCGT+AGG 0.620944 4.4:-45663204 MS.gene007715:CDS
TTGTTTGGGAGGGCTAGAGA+AGG 0.621430 4.4:+45664708 None:intergenic
ACTGGATGTTATTGGTGGAG+AGG 0.622948 4.4:+45664505 None:intergenic
AAACTTTAGACAATGAGGGG+TGG 0.625674 4.4:-45662564 MS.gene007715:CDS
CGAGGTCCCAACATGATGCA+AGG 0.625710 4.4:-45663069 MS.gene007715:intron
GTACGGTGGTTTCAATGGGG+AGG 0.625711 4.4:-45663722 MS.gene007715:CDS
TCCATCAATTCCTTCCCAAG+AGG 0.631113 4.4:+45663549 None:intergenic
AACAAACTTTAGACAATGAG+GGG 0.631532 4.4:-45662567 MS.gene007715:CDS
AATCCAAACACATGAAACAT+CGG 0.641573 4.4:+45663780 None:intergenic
TGCTTTGGCGAAACAGAGTG+TGG 0.643161 4.4:-45663622 MS.gene007715:CDS
CATGGACGATGATTTAGCAG+GGG 0.652854 4.4:-45663832 MS.gene007715:CDS
TCTTGGGAAGGAATTGATGG+AGG 0.654495 4.4:-45663547 MS.gene007715:CDS
AAAGAACTGATCAAATACAA+AGG 0.656643 4.4:-45662478 MS.gene007715:CDS
ACATACGCGCAGCTAAGGAG+AGG 0.659076 4.4:-45663747 MS.gene007715:CDS
ACCTGATAAATAGTAACATG+AGG 0.666648 4.4:+45663507 None:intergenic
ACTCCCTCCAAAACAATTGG+GGG 0.667004 4.4:-45663103 MS.gene007715:CDS
AGGTTATTATAACAATCCAG+AGG 0.669013 4.4:-45662596 MS.gene007715:CDS
GGTGACTATTGCACCGATGG+AGG 0.673270 4.4:+45664458 None:intergenic
AGTGGTGACTATTGCACCGA+TGG 0.675842 4.4:+45664455 None:intergenic
GCCAAAGCAAGAATCATCGG+TGG 0.703214 4.4:+45663636 None:intergenic
AGCGGCGTTAATGATTAACA+TGG 0.703668 4.4:-45663850 MS.gene007715:CDS
GAAGTACGGTGGTTTCAATG+GGG 0.715971 4.4:-45663725 MS.gene007715:CDS
TTTGAAACAGACCGATACAG+CGG 0.717255 4.4:-45663943 MS.gene007715:CDS
GGTGTAAAGAGTATTTGCAG+TGG 0.726670 4.4:+45664437 None:intergenic
ACACAAGAACTTCATCGCAG+CGG 0.736714 4.4:-45663868 MS.gene007715:CDS
GCTAAGGAGAGGAAGTACGG+TGG 0.774505 4.4:-45663736 MS.gene007715:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATAGAGAAATATCAAT+TGG + chr4.4:45664330-45664349 None:intergenic 15.0%
!! GTTTAAAAATTAGTTGAAAT+TGG + chr4.4:45664635-45664654 None:intergenic 15.0%
!! TAACAAACTTTATATCTAAT+AGG - chr4.4:45662225-45662244 MS.gene007715:intron 15.0%
!! AATAACCTGAAATATTGTAT+AGG + chr4.4:45663670-45663689 None:intergenic 20.0%
!! ACAATATAATTGTATTCTGT+TGG + chr4.4:45663290-45663309 None:intergenic 20.0%
!! ACCATTAAAAACATAAATAC+AGG + chr4.4:45664593-45664612 None:intergenic 20.0%
!! ATTGATTATCTTGTACTATT+AGG - chr4.4:45663019-45663038 MS.gene007715:intron 20.0%
!! CCATTAAAAACATAAATACA+GGG + chr4.4:45664592-45664611 None:intergenic 20.0%
!! GATCTTAAGAAATATTAATG+AGG + chr4.4:45663983-45664002 None:intergenic 20.0%
!! TTAATATTTCTTAAGATCGA+TGG - chr4.4:45663985-45664004 MS.gene007715:CDS 20.0%
!! TTGATTATCTTGTACTATTA+GGG - chr4.4:45663020-45663039 MS.gene007715:intron 20.0%
!!! AATTAAAGTATCTTGATCAT+TGG - chr4.4:45662912-45662931 MS.gene007715:intron 20.0%
!!! TAATTAGTTGTTTTCTTCAT+TGG - chr4.4:45662080-45662099 MS.gene007715:intron 20.0%
!!! TAATTTTTAAACACGCTTAT+AGG - chr4.4:45664643-45664662 MS.gene007715:CDS 20.0%
!!! TGTATTTATGTTTTTAATGG+TGG - chr4.4:45664592-45664611 MS.gene007715:CDS 20.0%
!!! TGTTATTTAAAAGCATGAAA+AGG + chr4.4:45663501-45663520 None:intergenic 20.0%
! AAAACAAACTTTAGACAATG+AGG - chr4.4:45663709-45663728 MS.gene007715:CDS 25.0%
! AAACAAACTTTAGACAATGA+GGG - chr4.4:45663710-45663729 MS.gene007715:CDS 25.0%
! AAAGAACTGATCAAATACAA+AGG - chr4.4:45663800-45663819 MS.gene007715:CDS 25.0%
! AATCACTTAGAGTTATTTGT+AGG + chr4.4:45663265-45663284 None:intergenic 25.0%
! ACCATATAAATATACTGTGA+TGG - chr4.4:45664288-45664307 MS.gene007715:intron 25.0%
! ATATCTAATAGGTAATGCTA+CGG - chr4.4:45662236-45662255 MS.gene007715:intron 25.0%
! ATGTTACTATTTATCAGGTA+AGG - chr4.4:45662775-45662794 MS.gene007715:intron 25.0%
! GTATAGGAAAAGTTTCATAT+GGG + chr4.4:45663654-45663673 None:intergenic 25.0%
! TAAAATCGCTATCTTTAATC+CGG + chr4.4:45662878-45662897 None:intergenic 25.0%
! TATATCTCCAACTTTATGAT+TGG - chr4.4:45663615-45663634 MS.gene007715:CDS 25.0%
! TCCATCACAGTATATTTATA+TGG + chr4.4:45664292-45664311 None:intergenic 25.0%
! TGATTATCTTGTACTATTAG+GGG - chr4.4:45663021-45663040 MS.gene007715:intron 25.0%
! TGTATAGGAAAAGTTTCATA+TGG + chr4.4:45663655-45663674 None:intergenic 25.0%
! TTACATTCCAATCATAAAGT+TGG + chr4.4:45663625-45663644 None:intergenic 25.0%
!! AGTGATAACTAATTTAGGAT+TGG + chr4.4:45661898-45661917 None:intergenic 25.0%
!! ATATCCTAACTTGTAACAAT+TGG - chr4.4:45664225-45664244 MS.gene007715:intron 25.0%
!! CTTTTCCTATACAATATTTC+AGG - chr4.4:45663662-45663681 MS.gene007715:CDS 25.0%
!!! ATTGTCTAAAGTTTGTTTTG+TGG + chr4.4:45663708-45663727 None:intergenic 25.0%
!!! CCCTGTATTTATGTTTTTAA+TGG - chr4.4:45664589-45664608 MS.gene007715:CDS 25.0%
!!! TTGTCTCTTTGAAATTAACT+AGG - chr4.4:45664072-45664091 MS.gene007715:intron 25.0%
!!! TTTCTTCATTGAAGAGTATT+GGG - chr4.4:45662694-45662713 MS.gene007715:intron 25.0%
AAACTTCTGAATTTCTTCCT+CGG + chr4.4:45664451-45664470 None:intergenic 30.0%
AACAAACTTTAGACAATGAG+GGG - chr4.4:45663711-45663730 MS.gene007715:CDS 30.0%
AAGTAAACATAACAGTGACA+AGG + chr4.4:45663914-45663933 None:intergenic 30.0%
AATCCAAACACATGAAACAT+CGG + chr4.4:45662501-45662520 None:intergenic 30.0%
AATTTACCTTGCATCATGTT+GGG + chr4.4:45663218-45663237 None:intergenic 30.0%
ACCTCATGTTACTATTTATC+AGG - chr4.4:45662770-45662789 MS.gene007715:intron 30.0%
ACCTGATAAATAGTAACATG+AGG + chr4.4:45662774-45662793 None:intergenic 30.0%
AGGTTATTATAACAATCCAG+AGG - chr4.4:45663682-45663701 MS.gene007715:CDS 30.0%
AGTATAGAGTGACAAATTTG+TGG - chr4.4:45662613-45662632 MS.gene007715:intron 30.0%
CAAACCAATTGTTACAAGTT+AGG + chr4.4:45664232-45664251 None:intergenic 30.0%
GATATAAAAACTGCAAGACT+TGG + chr4.4:45663601-45663620 None:intergenic 30.0%
GATCAAATACAAAGGTTATC+AGG - chr4.4:45663808-45663827 MS.gene007715:CDS 30.0%
GGTACAGTGATAACTAATTT+AGG + chr4.4:45661903-45661922 None:intergenic 30.0%
GTATAGAGTGACAAATTTGT+GGG - chr4.4:45662614-45662633 MS.gene007715:intron 30.0%
TACGATCAACAACATAAAGT+TGG + chr4.4:45663778-45663797 None:intergenic 30.0%
TATCTAATAGGTAATGCTAC+GGG - chr4.4:45662237-45662256 MS.gene007715:intron 30.0%
TCAGAAGTTTATTGCTAATC+AGG - chr4.4:45664461-45664480 MS.gene007715:CDS 30.0%
TGTTATGTTTACTTGAGCAT+TGG - chr4.4:45663920-45663939 MS.gene007715:CDS 30.0%
TTAGTCTAACTATAACCAAC+TGG + chr4.4:45662063-45662082 None:intergenic 30.0%
TTATCACTGTACCTGAATTA+TGG - chr4.4:45661910-45661929 MS.gene007715:intron 30.0%
TTGATCAAGAGAAGTTTCAT+TGG + chr4.4:45663449-45663468 None:intergenic 30.0%
TTGGAAAGATGATATGGTAT+TGG - chr4.4:45663939-45663958 MS.gene007715:CDS 30.0%
! AGTGTTCTTGTTATGCTTTA+AGG - chr4.4:45663316-45663335 MS.gene007715:intron 30.0%
! GTTTCTTCATTGAAGAGTAT+TGG - chr4.4:45662693-45662712 MS.gene007715:intron 30.0%
! TATCATGCAAGTTTTACTCA+CGG + chr4.4:45664163-45664182 None:intergenic 30.0%
! TGGATTTTCGATAGAAACAA+CGG + chr4.4:45663069-45663088 None:intergenic 30.0%
! TGGCTATATATTTGTTGCAT+TGG - chr4.4:45662100-45662119 MS.gene007715:intron 30.0%
!! ATTGGAATCCTTGATTTGTT+TGG + chr4.4:45661880-45661899 None:intergenic 30.0%
!! TAAATCATTTTTATCCCAGC+TGG + chr4.4:45662992-45663011 None:intergenic 30.0%
!!! CTTGGATATTTTGATGAGAT+CGG - chr4.4:45663752-45663771 MS.gene007715:CDS 30.0%
!!! TACGAGGATTATTTTTGACA+AGG - chr4.4:45664029-45664048 MS.gene007715:CDS 30.0%
AAAGTCTCTGTAGAGTACAA+TGG - chr4.4:45663561-45663580 MS.gene007715:CDS 35.0%
AAATACTCTTTACACCGTTG+CGG - chr4.4:45661848-45661867 MS.gene007715:CDS 35.0%
AAATCTGGTTATAGTCCAGA+TGG - chr4.4:45661528-45661547 MS.gene007715:CDS 35.0%
AAGGAGATGTTTGGATTGTT+TGG + chr4.4:45661588-45661607 None:intergenic 35.0%
AATGAGACTAAGGAGATGTT+TGG + chr4.4:45661597-45661616 None:intergenic 35.0%
ACATAACAGTGACAAGGATT+TGG + chr4.4:45663908-45663927 None:intergenic 35.0%
ACCATTCAAAAGATTGCGAA+GGG - chr4.4:45664670-45664689 MS.gene007715:CDS 35.0%
ACCGACTGATCTTGATAAAA+CGG + chr4.4:45662855-45662874 None:intergenic 35.0%
AGGAGATGTTTGGATTGTTT+GGG + chr4.4:45661587-45661606 None:intergenic 35.0%
ATAGTCCAGATGGAATATAC+AGG - chr4.4:45661538-45661557 MS.gene007715:CDS 35.0%
ATCTTGTACTATTAGGGGTA+TGG - chr4.4:45663026-45663045 MS.gene007715:intron 35.0%
CAATGACACATTCAGATCAT+TGG + chr4.4:45662949-45662968 None:intergenic 35.0%
CAATTTACCTTGCATCATGT+TGG + chr4.4:45663219-45663238 None:intergenic 35.0%
CATTGCATTGAACCTATTTG+AGG - chr4.4:45662964-45662983 MS.gene007715:intron 35.0%
GATGTTTCATGTGTTTGGAT+TGG - chr4.4:45662500-45662519 MS.gene007715:CDS 35.0%
GCATGATAGTTTACGTTTGA+GGG - chr4.4:45664175-45664194 MS.gene007715:intron 35.0%
TAATCTTGATCGGACAGTTA+GGG - chr4.4:45662798-45662817 MS.gene007715:intron 35.0%
TACAGGAAACTGGATGTTAT+TGG + chr4.4:45661784-45661803 None:intergenic 35.0%
TCAGGTAAGGTAATCTTGAT+CGG - chr4.4:45662788-45662807 MS.gene007715:intron 35.0%
TGCATGATAGTTTACGTTTG+AGG - chr4.4:45664174-45664193 MS.gene007715:intron 35.0%
TTCACTTTGCTCCATAATTC+AGG + chr4.4:45661924-45661943 None:intergenic 35.0%
! AAGAAGATCTTCAAGTTCTG+CGG + chr4.4:45664104-45664123 None:intergenic 35.0%
! ATCATGCAAGTTTTACTCAC+GGG + chr4.4:45664162-45664181 None:intergenic 35.0%
! ATTTTTCCTGAAGCTGTCTT+AGG + chr4.4:45664716-45664735 None:intergenic 35.0%
! CATATTTCCCCCAATTGTTT+TGG + chr4.4:45663185-45663204 None:intergenic 35.0%
! GTCTGTTTCAAATCGTTTTC+TGG + chr4.4:45662327-45662346 None:intergenic 35.0%
! TTCTAACTTCACGAGTTTTG+AGG - chr4.4:45662266-45662285 MS.gene007715:intron 35.0%
! TTTCTTAAGATCGATGGACT+AGG - chr4.4:45663991-45664010 MS.gene007715:CDS 35.0%
!! CAAGCTTTAAACTATGTCTG+AGG - chr4.4:45664549-45664568 MS.gene007715:CDS 35.0%
!! CTTTTGCTGTAACTTGTAAG+TGG + chr4.4:45664517-45664536 None:intergenic 35.0%
!! TGAGCATTGGAAAGATGATA+TGG - chr4.4:45663933-45663952 MS.gene007715:CDS 35.0%
!!! CGTCGTTTTTATTTATGCCA+AGG + chr4.4:45661743-45661762 None:intergenic 35.0%
!!! TTGTGTTTTGAGTTGAGAGA+AGG + chr4.4:45661691-45661710 None:intergenic 35.0%
AAACTTTAGACAATGAGGGG+TGG - chr4.4:45663714-45663733 MS.gene007715:CDS 40.0%
AACATGGACGATGATTTAGC+AGG - chr4.4:45662444-45662463 MS.gene007715:intron 40.0%
AACTTTAGACAATGAGGGGT+GGG - chr4.4:45663715-45663734 MS.gene007715:CDS 40.0%
ACATGGACGATGATTTAGCA+GGG - chr4.4:45662445-45662464 MS.gene007715:intron 40.0%
ACATTCACGAATTCGGACAT+CGG - chr4.4:45662826-45662845 MS.gene007715:intron 40.0%
ACGACAGCATCTTGTATTTC+AGG + chr4.4:45664136-45664155 None:intergenic 40.0%
AGATGTTTGGATTGTTTGGG+AGG + chr4.4:45661584-45661603 None:intergenic 40.0%
AGCGGCGTTAATGATTAACA+TGG - chr4.4:45662428-45662447 MS.gene007715:intron 40.0%
AGGAAACTGGATGTTATTGG+TGG + chr4.4:45661781-45661800 None:intergenic 40.0%
ATAACCAACTGGTAGCAGTA+TGG + chr4.4:45662052-45662071 None:intergenic 40.0%
CACCATTCAAAAGATTGCGA+AGG - chr4.4:45664669-45664688 MS.gene007715:CDS 40.0%
CCTGCAATTATCATCAACTC+AGG - chr4.4:45661963-45661982 MS.gene007715:intron 40.0%
CGACAGCATCTTGTATTTCA+GGG + chr4.4:45664135-45664154 None:intergenic 40.0%
CTAGGAAACATACAGGAAAC+TGG + chr4.4:45661794-45661813 None:intergenic 40.0%
GAAATCATGTTGTGGTTACG+AGG - chr4.4:45664013-45664032 MS.gene007715:CDS 40.0%
GAACGAACAACATAGGCAAT+TGG + chr4.4:45664411-45664430 None:intergenic 40.0%
GATGTTTGGATTGTTTGGGA+GGG + chr4.4:45661583-45661602 None:intergenic 40.0%
GGAACTCTCCAAACAAATCA+AGG - chr4.4:45661869-45661888 MS.gene007715:CDS 40.0%
GTAATCTTGATCGGACAGTT+AGG - chr4.4:45662797-45662816 MS.gene007715:intron 40.0%
TCTATCGAAAATCCAAGCGT+AGG - chr4.4:45663074-45663093 MS.gene007715:CDS 40.0%
TGTGAAGAGGAATGAGACTA+AGG + chr4.4:45661607-45661626 None:intergenic 40.0%
TTATAACATACGCGCAGCTA+AGG - chr4.4:45662526-45662545 MS.gene007715:CDS 40.0%
TTCGCCAAAGCAAGAATCAT+CGG + chr4.4:45662648-45662667 None:intergenic 40.0%
TTGCCGATGTTTCATGTGTT+TGG - chr4.4:45662495-45662514 MS.gene007715:CDS 40.0%
TTTATCAAGATCAGTCGGTC+CGG - chr4.4:45662856-45662875 MS.gene007715:intron 40.0%
! ACCCTTCGCAATCTTTTGAA+TGG + chr4.4:45664674-45664693 None:intergenic 40.0%
! AGAAGATCTTCAAGTTCTGC+GGG + chr4.4:45664103-45664122 None:intergenic 40.0%
! CTATAGATTTCCTGATGACG+AGG - chr4.4:45664374-45664393 MS.gene007715:CDS 40.0%
! GTTTCATGTGTTTGGATTGG+CGG - chr4.4:45662503-45662522 MS.gene007715:CDS 40.0%
! TGAGTTAGAGGGGTTTTTGA+AGG - chr4.4:45662581-45662600 MS.gene007715:CDS 40.0%
! TGGACTAGGAAATCATGTTG+TGG - chr4.4:45664005-45664024 MS.gene007715:CDS 40.0%
! TTTGAAACAGACCGATACAG+CGG - chr4.4:45662335-45662354 MS.gene007715:intron 40.0%
!! CCTGAGTTGATGATAATTGC+AGG + chr4.4:45661966-45661985 None:intergenic 40.0%
!! GCCGTTTTATCAAGATCAGT+CGG - chr4.4:45662851-45662870 MS.gene007715:intron 40.0%
!! GGTGTAAAGAGTATTTGCAG+TGG + chr4.4:45661844-45661863 None:intergenic 40.0%
!! TCATTGAAGAGTATTGGGTC+TGG - chr4.4:45662699-45662718 MS.gene007715:intron 40.0%
!! TTTGAGTTGAGAGAAGGAGA+GGG + chr4.4:45661685-45661704 None:intergenic 40.0%
!!! AAAGTTTGTTTTGTGGCCTC+TGG + chr4.4:45663701-45663720 None:intergenic 40.0%
!!! ATTTCCCCCAATTGTTTTGG+AGG + chr4.4:45663182-45663201 None:intergenic 40.0%
!!! TTTCCCCCAATTGTTTTGGA+GGG + chr4.4:45663181-45663200 None:intergenic 40.0%
!!! TTTTGAGTTGAGAGAAGGAG+AGG + chr4.4:45661686-45661705 None:intergenic 40.0%
AAGAAGAGACGCTTGTGAAG+AGG + chr4.4:45661620-45661639 None:intergenic 45.0%
ACACAAGAACTTCATCGCAG+CGG - chr4.4:45662410-45662429 MS.gene007715:intron 45.0%
ACTCCCTCCAAAACAATTGG+GGG - chr4.4:45663175-45663194 MS.gene007715:CDS 45.0%
ACTGGATGTTATTGGTGGAG+AGG + chr4.4:45661776-45661795 None:intergenic 45.0%
AGCGTAGGTATTCGTCATTC+TGG - chr4.4:45663089-45663108 MS.gene007715:CDS 45.0%
AGGGACCTGTATATTCCATC+TGG + chr4.4:45661546-45661565 None:intergenic 45.0%
AGTGTTCCTAAGACAGCTTC+AGG - chr4.4:45664707-45664726 MS.gene007715:CDS 45.0%
CACTCCCTCCAAAACAATTG+GGG - chr4.4:45663174-45663193 MS.gene007715:CDS 45.0%
CATGGACGATGATTTAGCAG+GGG - chr4.4:45662446-45662465 MS.gene007715:intron 45.0%
CCACTCCCTCCAAAACAATT+GGG - chr4.4:45663173-45663192 MS.gene007715:CDS 45.0%
CGAAGCTCAAATAGTGAGTG+TGG - chr4.4:45663139-45663158 MS.gene007715:CDS 45.0%
CTGTACTCATCAGGAACTAC+AGG - chr4.4:45662363-45662382 MS.gene007715:intron 45.0%
GAAGTACGGTGGTTTCAATG+GGG - chr4.4:45662553-45662572 MS.gene007715:CDS 45.0%
GAATGACGAATACCTACGCT+TGG + chr4.4:45663089-45663108 None:intergenic 45.0%
GCACACAGTTTACTCAACCT+TGG - chr4.4:45661723-45661742 MS.gene007715:intron 45.0%
GGAGCACACATTCACGAATT+CGG - chr4.4:45662819-45662838 MS.gene007715:intron 45.0%
GTTGGGTGAACGAACAACAT+AGG + chr4.4:45664418-45664437 None:intergenic 45.0%
TCCATCAATTCCTTCCCAAG+AGG + chr4.4:45662732-45662751 None:intergenic 45.0%
TCCTCTTGGGAAGGAATTGA+TGG - chr4.4:45662728-45662747 MS.gene007715:intron 45.0%
TCTTGGGAAGGAATTGATGG+AGG - chr4.4:45662731-45662750 MS.gene007715:intron 45.0%
TGGGTACATACAGGAGATCT+TGG - chr4.4:45663734-45663753 MS.gene007715:CDS 45.0%
TGTTATTGGTGGAGAGGATG+AGG + chr4.4:45661770-45661789 None:intergenic 45.0%
TTCACCATACTGCTACCAGT+TGG - chr4.4:45662045-45662064 MS.gene007715:intron 45.0%
TTGAGAGAAGGAGAGGGTTT+GGG + chr4.4:45661679-45661698 None:intergenic 45.0%
TTGTTTGGAGAGTTCCGCAA+CGG + chr4.4:45661865-45661884 None:intergenic 45.0%
! AATGGGGAGGTTTGAGTTAG+AGG - chr4.4:45662569-45662588 MS.gene007715:CDS 45.0%
! AGGAAGTACGGTGGTTTCAA+TGG - chr4.4:45662551-45662570 MS.gene007715:CDS 45.0%
! ATGGGGAGGTTTGAGTTAGA+GGG - chr4.4:45662570-45662589 MS.gene007715:CDS 45.0%
! GGAAGTACGGTGGTTTCAAT+GGG - chr4.4:45662552-45662571 MS.gene007715:CDS 45.0%
!! TCAGCTGGAATGTTGGTTTC+AGG - chr4.4:45663113-45663132 MS.gene007715:CDS 45.0%
AATGAGGGGTGGGTACATAC+AGG - chr4.4:45663725-45663744 MS.gene007715:CDS 50.0%
AGTGGTGACTATTGCACCGA+TGG + chr4.4:45661826-45661845 None:intergenic 50.0%
GAGGGCTAGAGAAGGTCTTA+GGG + chr4.4:45661565-45661584 None:intergenic 50.0%
GCAGCTAAGGAGAGGAAGTA+CGG - chr4.4:45662539-45662558 MS.gene007715:CDS 50.0%
GCCAAAGCAAGAATCATCGG+TGG + chr4.4:45662645-45662664 None:intergenic 50.0%
GCCACCGATGATTCTTGCTT+TGG - chr4.4:45662641-45662660 MS.gene007715:intron 50.0%
GCCACTCCCTCCAAAACAAT+TGG - chr4.4:45663172-45663191 MS.gene007715:CDS 50.0%
GGAACTACAGGAGTGAGCAA+AGG - chr4.4:45662375-45662394 MS.gene007715:intron 50.0%
GTGGCAGCTAGGAAACATAC+AGG + chr4.4:45661801-45661820 None:intergenic 50.0%
GTTGAGAGAAGGAGAGGGTT+TGG + chr4.4:45661680-45661699 None:intergenic 50.0%
TCCTCGGACAGCAAACTGTT+GGG + chr4.4:45664435-45664454 None:intergenic 50.0%
TGCTTTGGCGAAACAGAGTG+TGG - chr4.4:45662656-45662675 MS.gene007715:intron 50.0%
TGGCGAAACAGAGTGTGGTT+GGG - chr4.4:45662661-45662680 MS.gene007715:intron 50.0%
TTCCTCGGACAGCAAACTGT+TGG + chr4.4:45664436-45664455 None:intergenic 50.0%
TTGGCGAAACAGAGTGTGGT+TGG - chr4.4:45662660-45662679 MS.gene007715:intron 50.0%
TTGGGGGAAATATGCGTACG+AGG - chr4.4:45663191-45663210 MS.gene007715:CDS 50.0%
TTGTTTGGGAGGGCTAGAGA+AGG + chr4.4:45661573-45661592 None:intergenic 50.0%
! AACCTATTTGAGGCACCAGC+TGG - chr4.4:45662974-45662993 MS.gene007715:intron 50.0%
! ACCTATTTGAGGCACCAGCT+GGG - chr4.4:45662975-45662994 MS.gene007715:intron 50.0%
! AGATTTCCTGATGACGAGGC+TGG - chr4.4:45664378-45664397 MS.gene007715:CDS 50.0%
! GTCGATGAGGGCGATTTTGT+CGG + chr4.4:45661646-45661665 None:intergenic 50.0%
! TCGATGAGGGCGATTTTGTC+GGG + chr4.4:45661645-45661664 None:intergenic 50.0%
! TGGGGAGGTTTGAGTTAGAG+GGG - chr4.4:45662571-45662590 MS.gene007715:CDS 50.0%
!! ATTCGTCATTCTGGCTCAGC+TGG - chr4.4:45663098-45663117 MS.gene007715:CDS 50.0%
!! GAGGCGTTGATTGAATCAGC+AGG - chr4.4:45662285-45662304 MS.gene007715:intron 50.0%
!! GTACAGAAGTGCCGCTGTAT+CGG + chr4.4:45662349-45662368 None:intergenic 50.0%
!! TTCTGGCTCAGCTGGAATGT+TGG - chr4.4:45663106-45663125 MS.gene007715:CDS 50.0%
!!! CCCAATTGTTTTGGAGGGAG+TGG + chr4.4:45663176-45663195 None:intergenic 50.0%
ACATACGCGCAGCTAAGGAG+AGG - chr4.4:45662531-45662550 MS.gene007715:CDS 55.0%
CGAGGTCCCAACATGATGCA+AGG - chr4.4:45663209-45663228 MS.gene007715:CDS 55.0%
GCGGCACTTCTGTACTCATC+AGG - chr4.4:45662354-45662373 MS.gene007715:intron 55.0%
GGAGGGCTAGAGAAGGTCTT+AGG + chr4.4:45661566-45661585 None:intergenic 55.0%
GTACGGTGGTTTCAATGGGG+AGG - chr4.4:45662556-45662575 MS.gene007715:CDS 55.0%
TCCCAGCTGGTGCCTCAAAT+AGG + chr4.4:45662979-45662998 None:intergenic 55.0%
TTCCTAGCTGCCACCTCCAT+CGG - chr4.4:45661807-45661826 MS.gene007715:intron 55.0%
! CGATGAGGGCGATTTTGTCG+GGG + chr4.4:45661644-45661663 None:intergenic 55.0%
! GACTATTGCACCGATGGAGG+TGG + chr4.4:45661820-45661839 None:intergenic 55.0%
! GATGAGGGCGATTTTGTCGG+GGG + chr4.4:45661643-45661662 None:intergenic 55.0%
! GCTAAGGAGAGGAAGTACGG+TGG - chr4.4:45662542-45662561 MS.gene007715:CDS 55.0%
! GGAAGAGTCTGCGTCGATGA+GGG + chr4.4:45661658-45661677 None:intergenic 55.0%
! GGTGACTATTGCACCGATGG+AGG + chr4.4:45661823-45661842 None:intergenic 55.0%
!! ACCCAACAGTTTGCTGTCCG+AGG - chr4.4:45664431-45664450 MS.gene007715:CDS 55.0%
ACCTCACCAGCCTCGTCATC+AGG + chr4.4:45664387-45664406 None:intergenic 60.0%
! GGGAAGAGTCTGCGTCGATG+AGG + chr4.4:45661659-45661678 None:intergenic 60.0%
! TCCTGATGACGAGGCTGGTG+AGG - chr4.4:45664383-45664402 MS.gene007715:CDS 60.0%
!! GGTCTGGTGCTGCTCCTCTT+GGG - chr4.4:45662715-45662734 MS.gene007715:intron 60.0%
!! TGGTGCTGCTCCTCTTGGGA+AGG - chr4.4:45662719-45662738 MS.gene007715:intron 60.0%
CACCGATGGAGGTGGCAGCT+AGG + chr4.4:45661812-45661831 None:intergenic 65.0%
!! GGGTCTGGTGCTGCTCCTCT+TGG - chr4.4:45662714-45662733 MS.gene007715:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 45661522 45664778 45661522 ID=MS.gene007715
chr4.4 mRNA 45661522 45664778 45661522 ID=MS.gene007715.t1;Parent=MS.gene007715
chr4.4 exon 45664316 45664778 45664316 ID=MS.gene007715.t1.exon1;Parent=MS.gene007715.t1
chr4.4 CDS 45664316 45664778 45664316 ID=cds.MS.gene007715.t1;Parent=MS.gene007715.t1
chr4.4 exon 45663509 45664053 45663509 ID=MS.gene007715.t1.exon2;Parent=MS.gene007715.t1
chr4.4 CDS 45663509 45664053 45663509 ID=cds.MS.gene007715.t1;Parent=MS.gene007715.t1
chr4.4 exon 45663070 45663259 45663070 ID=MS.gene007715.t1.exon3;Parent=MS.gene007715.t1
chr4.4 CDS 45663070 45663259 45663070 ID=cds.MS.gene007715.t1;Parent=MS.gene007715.t1
chr4.4 exon 45662471 45662616 45662471 ID=MS.gene007715.t1.exon4;Parent=MS.gene007715.t1
chr4.4 CDS 45662471 45662616 45662471 ID=cds.MS.gene007715.t1;Parent=MS.gene007715.t1
chr4.4 exon 45662139 45662206 45662139 ID=MS.gene007715.t1.exon5;Parent=MS.gene007715.t1
chr4.4 CDS 45662139 45662206 45662139 ID=cds.MS.gene007715.t1;Parent=MS.gene007715.t1
chr4.4 exon 45661818 45661920 45661818 ID=MS.gene007715.t1.exon6;Parent=MS.gene007715.t1
chr4.4 CDS 45661818 45661920 45661818 ID=cds.MS.gene007715.t1;Parent=MS.gene007715.t1
chr4.4 exon 45661522 45661635 45661522 ID=MS.gene007715.t1.exon7;Parent=MS.gene007715.t1
chr4.4 CDS 45661522 45661635 45661522 ID=cds.MS.gene007715.t1;Parent=MS.gene007715.t1
Gene Sequence

>MS.gene007715

ATGCAGAAATCTGGTTATAGTCCAGATGGAATATACAGGTCCCTAAGACCTTCTCTAGCCCTCCCAAACAATCCAAACATCTCCTTAGTCTCATTCCTCTTCACAAGCGTCTCTTCTTCCCCCGACAAAATCGCCCTCATCGACGCAGACTCTTCCCAAACCCTCTCCTTCTCTCAACTCAAAACACAAGTTGTAAAACTTGCACACAGTTTACTCAACCTTGGCATAAATAAAAACGACGTTGTCCTCATCCTCTCCACCAATAACATCCAGTTTCCTGTATGTTTCCTAGCTGCCACCTCCATCGGTGCAATAGTCACCACTGCAAATACTCTTTACACCGTTGCGGAACTCTCCAAACAAATCAAGGATTCCAATCCTAAATTAGTTATCACTGTACCTGAATTATGGAGCAAAGTGAAGAGTTTCAATTTTAATTTTCCTGCAATTATCATCAACTCAGGTAATATTTGATACAAAGTTAGTTATAGTTACTAGTTAGACTAGTTAGTTTTTGTCATCATTCACCATACTGCTACCAGTTGGTTATAGTTAGACTAATTAGTTGTTTTCTTCATTGGCTATATATTTGTTGCATCAGTTAGTTATAGTTAGACTAATTAGTTGTTTTCTTCATTGGCTATATATTTGTTGCATTGGTTAGTTTAGTTACACTAATTAGCTGTTCTCATCATTCTCTATATAACAAACTTTATATCTAATAGGTAATGCTACGGGTTTAAATTCTAACTTCACGAGTTTTGAGGCGTTGATTGAATCAGCAGGAGAAGTAACAGAGTTACCAGAAAACGATTTGAAACAGACCGATACAGCGGCACTTCTGTACTCATCAGGAACTACAGGAGTGAGCAAAGGAGTGATTCTCACACACAAGAACTTCATCGCAGCGGCGTTAATGATTAACATGGACGATGATTTAGCAGGGGAATTAAACAACGTGTTCATCTGCGTTTTGCCGATGTTTCATGTGTTTGGATTGGCGGTTATAACATACGCGCAGCTAAGGAGAGGAAGTACGGTGGTTTCAATGGGGAGGTTTGAGTTAGAGGGGTTTTTGAAGGTGATTGAGAAGTATAGAGTGACAAATTTGTGGGTTGTGCCACCGATGATTCTTGCTTTGGCGAAACAGAGTGTGGTTGGGAAATATGATGTTTCTTCATTGAAGAGTATTGGGTCTGGTGCTGCTCCTCTTGGGAAGGAATTGATGGAGGAGTGTGCTCGACGTCTACCTCATGTTACTATTTATCAGGTAAGGTAATCTTGATCGGACAGTTAGGGAGCACACATTCACGAATTCGGACATCGGTAGCCGTTTTATCAAGATCAGTCGGTCCGGATTAAAGATAGCGATTTTAAGTTATAAAACTGAAATTAAAGTATCTTGATCATTGGTAATTTTTTTACCAATGATCTGAATGTGTCATTGCATTGAACCTATTTGAGGCACCAGCTGGGATAAAAATGATTTATTTATTAATTGATTATCTTGTACTATTAGGGGTATGGTATGACAGAAACTTGTGCCGTTGTTTCTATCGAAAATCCAAGCGTAGGTATTCGTCATTCTGGCTCAGCTGGAATGTTGGTTTCAGGAGTCGAAGCTCAAATAGTGAGTGTGGATACACAGAAGCCACTCCCTCCAAAACAATTGGGGGAAATATGCGTACGAGGTCCCAACATGATGCAAGGTAAATTGTCAGCACATTCAACATTTACACTCCTACAAATAACTCTAAGTGATTTTCCAACAGAATACAATTATATTGTACATGTAGTGTTCTTGTTATGCTTTAAGGAATTCAGTAAAAAAAATGACTTGCATTGTTGTATATGCTACTTTCTGATATACATCTATTTTTTCTATTAAAAAATTCAACTTCTTAGTGTATTTGTAAATTCTCTACCAATGAAACTTCTCTTGATCAACTAATAATTAGAAACATGAACTTTAAGTTCCTTTTCATGCTTTTAAATAACATTGTAATCATAATGCATTTTATGATCATACTTACTTGTACAAAGTCTCTGTAGAGTACAATGGCTAGCACTGTTTCTCCAAGTCTTGCAGTTTTTATATCTCCAACTTTATGATTGGAATGTAAATTTTTCCCATATGAAACTTTTCCTATACAATATTTCAGGTTATTATAACAATCCAGAGGCCACAAAACAAACTTTAGACAATGAGGGGTGGGTACATACAGGAGATCTTGGATATTTTGATGAGATCGGCCAACTTTATGTTGTTGATCGTATCAAAGAACTGATCAAATACAAAGGTTATCAGGTATGTAATCTAAAATGATATCGATTGTAGTAACTAAAGTAACATATTTCATCTATTCATTATCTGAAATCTTAGCCAAATCCTTGTCACTGTTATGTTTACTTGAGCATTGGAAAGATGATATGGTATTGGTTGTATTAATCAAATGATCCTCATTAATATTTCTTAAGATCGATGGACTAGGAAATCATGTTGTGGTTACGAGGATTATTTTTGACAAGGAATTTCACATTATGTAATATTTGTCTCTTTGAAATTAACTAGGTTGCGCCCGCAGAACTTGAAGATCTTCTTGTTTCTCACCCTGAAATACAAGATGCTGTCGTTATCCCGTGAGTAAAACTTGCATGATAGTTTACGTTTGAGGGATGTTACTTACATCAAAAAATTGAAAAATATCCTAACTTGTAACAATTGGTTTGACACAGAGAAAAAAGTAATAAAAAATGAAATCGTACACCATATAAATATACTGTGATGGACAACAAAACTGTTTTCCAATTGATATTTCTCTATTAATAATTCACATTTGAGTTACTTATTGCTATAGATTTCCTGATGACGAGGCTGGTGAGGTTCCAATTGCCTATGTTGTTCGTTCACCCAACAGTTTGCTGTCCGAGGAAGAAATTCAGAAGTTTATTGCTAATCAGGTGAGAAAAAGCTAAGTAACAAGTTTTATCTCCACTTACAAGTTACAGCAAAAGAAACACATTTTGCAAGCTTTAAACTATGTCTGAGGAATGAAACTGTTTTTAACCCTGTATTTATGTTTTTAATGGTGGTCAACTTATTTCATTTGCCAATTTCAACTAATTTTTAAACACGCTTATAGGTTGCACCATTCAAAAGATTGCGAAGGGTTACATTCATCAACAGTGTTCCTAAGACAGCTTCAGGAAAAATTCTCAGAAGAGAGCTTATTGCAAAAGCACGATCCAAAATATAA
Protein sequence

>MS.gene007715.t1

MQKSGYSPDGIYRSLRPSLALPNNPNISLVSFLFTSVSSSPDKIALIDADSSQTLSFSQLKTQVVKLAHSLLNLGINKNDVVLILSTNNIQFPVCFLAATSIGAIVTTANTLYTVAELSKQIKDSNPKLVITVPELWSKVKSFNFNFPAIIINSGNATGLNSNFTSFEALIESAGEVTELPENDLKQTDTAALLYSSGTTGVSKGVILTHKNFIAAALMINMDDDLAGELNNVFICVLPMFHVFGLAVITYAQLRRGSTVVSMGRFELEGFLKVIEKYRVTNLWVVPPMILALAKQSVVGKYDVSSLKSIGSGAAPLGKELMEECARRLPHVTIYQGYGMTETCAVVSIENPSVGIRHSGSAGMLVSGVEAQIVSVDTQKPLPPKQLGEICVRGPNMMQGYYNNPEATKQTLDNEGWVHTGDLGYFDEIGQLYVVDRIKELIKYKGYQVAPAELEDLLVSHPEIQDAVVIPFPDDEAGEVPIAYVVRSPNSLLSEEEIQKFIANQVAPFKRLRRVTFINSVPKTASGKILRRELIAKARSKI