AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04193

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04193.t1 MTR_3g460770 97.473 554 13 1 1 554 1 553 0.0 1105
MS.gene04193.t1 MTR_2g105570 39.154 544 311 9 10 538 7 545 2.29e-137 410
MS.gene04193.t1 MTR_4g005750 39.552 536 312 7 11 538 7 538 6.13e-130 390
MS.gene04193.t1 MTR_5g007640 37.893 541 315 8 11 538 51 583 9.46e-127 384
MS.gene04193.t1 MTR_4g128337 38.519 540 318 6 10 540 12 546 1.73e-121 369
MS.gene04193.t1 MTR_4g057730 35.424 542 332 8 13 545 9 541 2.16e-120 366
MS.gene04193.t1 MTR_2g095510 36.245 538 330 7 12 542 8 539 8.86e-117 356
MS.gene04193.t1 MTR_1g026310 34.608 549 317 9 8 532 20 550 7.07e-114 350
MS.gene04193.t1 MTR_1g090737 38.048 502 299 5 36 532 62 556 3.61e-113 348
MS.gene04193.t1 MTR_1g063120 34.926 544 320 10 23 542 22 555 2.54e-105 327
MS.gene04193.t1 MTR_8g039720 35.046 545 322 12 8 532 17 549 6.88e-103 321
MS.gene04193.t1 MTR_1g063110 34.026 529 334 8 16 532 13 538 4.97e-99 311
MS.gene04193.t1 MTR_4g077900 37.190 484 286 9 70 542 2 478 7.80e-99 308
MS.gene04193.t1 MTR_4g057730 34.615 468 292 7 13 471 9 471 6.50e-98 305
MS.gene04193.t1 MTR_5g027480 34.637 537 335 8 15 542 26 555 1.06e-97 307
MS.gene04193.t1 MTR_3g088870 34.800 500 300 8 44 532 64 548 8.88e-95 300
MS.gene04193.t1 MTR_3g088880 35.152 495 302 8 44 532 65 546 1.24e-94 300
MS.gene04193.t1 MTR_5g027500 33.945 545 340 8 15 542 27 568 4.51e-89 285
MS.gene04193.t1 MTR_4g057730 33.184 446 284 7 13 449 9 449 7.63e-87 276
MS.gene04193.t1 MTR_1g073180 35.433 508 313 9 34 539 37 531 1.60e-86 278
MS.gene04193.t1 MTR_2g095510 33.708 445 282 7 12 449 8 446 3.52e-82 264
MS.gene04193.t1 MTR_1g073180 34.927 481 298 9 34 512 59 526 6.71e-78 255
MS.gene04193.t1 MTR_3g035130 27.711 498 332 12 57 541 32 514 1.58e-53 190
MS.gene04193.t1 MTR_1569s0010 27.043 514 333 10 57 535 22 528 6.87e-53 188
MS.gene04193.t1 MTR_1g026430 28.500 400 244 9 8 383 20 401 1.32e-52 185
MS.gene04193.t1 MTR_1g026470 28.607 402 245 9 8 385 20 403 1.73e-52 184
MS.gene04193.t1 MTR_0803s0020 26.166 493 320 13 59 528 263 734 9.64e-43 164
MS.gene04193.t1 MTR_5g008800 26.277 548 357 16 34 553 19 547 1.48e-41 157
MS.gene04193.t1 MTR_3g035130 27.752 436 287 12 57 479 32 452 1.57e-41 156
MS.gene04193.t1 MTR_5g076240 28.185 518 317 17 58 536 41 542 1.83e-40 154
MS.gene04193.t1 MTR_3g064420 25.668 561 330 16 34 537 19 549 1.08e-39 152
MS.gene04193.t1 MTR_8g074040 23.596 534 363 13 34 537 61 579 2.91e-39 152
MS.gene04193.t1 MTR_2g044180 26.217 534 320 15 58 540 47 557 2.34e-38 149
MS.gene04193.t1 MTR_5g008820 24.953 537 348 15 34 537 19 533 6.70e-38 147
MS.gene04193.t1 MTR_4g121600 26.426 526 329 16 58 540 47 557 2.16e-35 140
MS.gene04193.t1 MTR_8g074120 28.101 395 250 14 160 537 113 490 2.85e-35 139
MS.gene04193.t1 MTR_8g074040 25.231 432 291 11 127 537 41 461 8.83e-35 137
MS.gene04193.t1 MTR_2g044180 27.842 431 249 13 149 540 59 466 2.30e-32 130
MS.gene04193.t1 MTR_5g023730 25.140 537 353 16 34 538 21 540 4.50e-32 130
MS.gene04193.t1 MTR_5g076220 25.184 544 347 17 34 536 19 543 8.81e-31 126
MS.gene04193.t1 MTR_5g076200 24.453 548 346 19 34 536 19 543 3.53e-28 119
MS.gene04193.t1 MTR_0161s0050 23.519 574 361 20 14 536 3 549 3.69e-26 112
MS.gene04193.t1 MTR_4g011790 25.676 370 241 12 192 536 233 593 5.24e-24 106
MS.gene04193.t1 MTR_4g011790 25.895 363 235 12 192 529 233 586 3.70e-23 103
MS.gene04193.t1 MTR_4g013210 24.665 373 245 11 192 536 76 440 4.32e-23 102
MS.gene04193.t1 MTR_2g030240 23.897 544 340 17 58 547 29 552 3.40e-22 100
MS.gene04193.t1 MTR_4g011790 25.330 379 240 13 192 536 233 602 9.07e-22 99.8
MS.gene04193.t1 MTR_4g013210 25.617 324 215 10 192 497 228 543 1.11e-20 96.3
MS.gene04193.t1 MTR_2g030240 24.685 397 241 12 192 547 79 458 1.75e-20 94.7
MS.gene04193.t1 MTR_4g057755 46.667 75 40 0 398 472 46 120 3.54e-20 87.0
MS.gene04193.t1 MTR_1054s0020 23.392 513 350 17 42 537 281 767 4.02e-20 94.7
MS.gene04193.t1 MTR_4g013210 23.967 363 219 10 192 536 76 399 4.70e-20 92.8
MS.gene04193.t1 MTR_7g084850 22.261 566 371 18 43 552 185 737 9.65e-20 93.6
MS.gene04193.t1 MTR_4g013210 23.784 370 225 10 185 536 221 551 1.26e-19 92.8
MS.gene04193.t1 MTR_1g104810 33.889 180 99 8 293 459 329 501 3.02e-19 92.0
MS.gene04193.t1 MTR_1g104810 33.155 187 105 8 286 459 389 568 3.24e-19 92.0
MS.gene04193.t1 MTR_7g109830 26.285 506 265 23 48 469 66 547 8.68e-19 90.5
MS.gene04193.t1 MTR_5g009360 26.389 360 202 16 162 469 75 423 2.44e-18 88.6
MS.gene04193.t1 MTR_1g026350 28.571 126 88 1 15 140 26 149 3.62e-18 82.8
MS.gene04193.t1 MTR_5g009360 26.389 360 202 16 162 469 201 549 4.27e-18 88.2
MS.gene04193.t1 MTR_8g083170 36.224 196 106 7 330 512 475 664 1.32e-17 87.0
MS.gene04193.t1 MTR_5g009350 26.536 358 204 14 162 469 201 549 3.34e-17 85.5
MS.gene04193.t1 MTR_5g009350 26.536 358 204 14 162 469 201 549 4.14e-17 85.1
MS.gene04193.t1 MTR_5g009350 26.462 359 205 14 162 470 201 550 4.51e-17 84.7
MS.gene04193.t1 MTR_5g009350 26.257 358 205 14 162 469 201 549 4.72e-17 84.7
MS.gene04193.t1 MTR_3g084470 36.000 175 98 5 330 493 468 639 5.18e-17 85.1
MS.gene04193.t1 MTR_8g099420 25.770 357 200 14 162 465 202 546 8.14e-17 84.3
MS.gene04193.t1 MTR_3g084450 32.673 202 114 7 330 518 264 456 9.82e-16 80.5
MS.gene04193.t1 MTR_3g084450 32.673 202 114 7 330 518 472 664 1.34e-15 80.5
MS.gene04193.t1 MTR_3g088890 48.333 60 31 0 444 503 10 69 2.92e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04193.t1 AT1G62940 72.932 532 141 2 10 539 7 537 0.0 790
MS.gene04193.t1 AT3G21240 38.060 536 319 6 12 538 19 550 7.81e-130 390
MS.gene04193.t1 AT1G65060 38.534 532 316 6 14 538 33 560 1.38e-126 382
MS.gene04193.t1 AT4G05160 36.531 542 326 8 13 542 9 544 3.83e-123 373
MS.gene04193.t1 AT1G20480 38.095 546 315 7 11 542 29 565 4.52e-123 374
MS.gene04193.t1 AT1G20510 35.755 523 325 7 16 532 17 534 4.08e-122 370
MS.gene04193.t1 AT3G21230 37.071 553 323 8 6 538 17 564 3.97e-118 361
MS.gene04193.t1 AT1G51680 37.291 539 318 8 15 538 24 557 3.77e-116 355
MS.gene04193.t1 AT3G21240 37.821 468 278 6 12 470 19 482 7.07e-108 332
MS.gene04193.t1 AT3G21240 37.821 468 278 6 12 470 19 482 7.07e-108 332
MS.gene04193.t1 AT5G63380 38.571 490 286 9 48 532 69 548 3.31e-106 330
MS.gene04193.t1 AT3G21230 36.484 529 305 9 6 514 17 534 3.63e-104 324
MS.gene04193.t1 AT1G51680 36.905 504 298 8 15 503 24 522 5.49e-103 321
MS.gene04193.t1 AT1G20500 31.895 533 347 8 8 532 16 540 2.43e-102 319
MS.gene04193.t1 AT4G19010 36.313 537 328 11 15 542 31 562 8.00e-102 318
MS.gene04193.t1 AT5G38120 32.902 541 335 9 16 542 24 550 1.40e-100 315
MS.gene04193.t1 AT1G20510 33.766 462 295 7 16 471 17 473 3.68e-98 306
MS.gene04193.t1 AT3G21230 36.495 485 283 8 6 470 17 496 1.26e-94 298
MS.gene04193.t1 AT1G51680 36.518 471 279 8 15 470 24 489 2.15e-93 294
MS.gene04193.t1 AT1G65060 35.281 445 277 6 14 451 33 473 8.03e-92 290
MS.gene04193.t1 AT5G38120 32.072 502 313 9 16 503 24 511 3.07e-87 279
MS.gene04193.t1 AT1G20510 32.045 440 288 7 16 449 17 451 9.24e-86 274
MS.gene04193.t1 AT5G38120 32.340 470 290 9 16 471 24 479 1.07e-79 258
MS.gene04193.t1 AT5G38120 32.340 470 290 9 16 471 24 479 1.07e-79 258
MS.gene04193.t1 AT3G21230 31.250 416 261 9 10 405 21 431 5.38e-53 187
MS.gene04193.t1 AT1G20490 27.635 427 279 10 8 418 16 428 5.50e-52 184
MS.gene04193.t1 AT1G20490 27.635 427 279 10 8 418 16 428 5.50e-52 184
MS.gene04193.t1 AT3G48990 28.407 521 343 12 35 541 9 513 1.58e-49 179
MS.gene04193.t1 AT1G21530 26.523 558 345 16 23 536 5 541 1.21e-41 157
MS.gene04193.t1 AT1G21530 26.523 558 347 16 23 536 5 543 4.50e-41 156
MS.gene04193.t1 AT5G16370 26.617 541 339 18 34 536 19 539 2.98e-40 154
MS.gene04193.t1 AT1G21540 26.476 559 347 15 23 536 5 544 8.03e-40 152
MS.gene04193.t1 AT5G16340 27.140 549 339 19 34 541 19 547 9.57e-40 152
MS.gene04193.t1 AT1G77240 25.698 537 359 16 29 536 14 539 4.00e-39 150
MS.gene04193.t1 AT1G75960 28.125 544 337 16 29 536 14 539 7.97e-39 150
MS.gene04193.t1 AT1G20560 27.491 542 334 17 34 537 21 541 2.89e-38 148
MS.gene04193.t1 AT2G17650 25.045 551 352 16 34 540 66 599 3.54e-37 145
MS.gene04193.t1 AT1G65890 26.151 543 350 15 34 536 19 550 5.23e-36 142
MS.gene04193.t1 AT1G65880 25.919 544 351 15 34 536 19 551 1.44e-35 140
MS.gene04193.t1 AT1G66120 24.953 537 357 14 34 536 19 543 1.44e-35 140
MS.gene04193.t1 AT1G20560 29.024 379 229 11 180 537 104 463 3.92e-32 129
MS.gene04193.t1 AT1G76290 27.222 540 341 19 34 542 19 537 3.78e-31 127
MS.gene04193.t1 AT3G16910 25.746 536 321 19 58 540 49 560 3.15e-30 125
MS.gene04193.t1 AT1G68270 24.304 539 325 16 34 531 19 515 3.19e-30 124
MS.gene04193.t1 AT5G27600 28.779 344 185 15 161 457 239 569 2.96e-25 110
MS.gene04193.t1 AT3G16170 21.529 497 351 10 70 538 119 604 1.38e-24 108
MS.gene04193.t1 AT3G05970 29.630 324 170 11 181 457 257 569 4.97e-23 103
MS.gene04193.t1 AT1G30520 22.563 554 350 13 47 543 23 554 2.64e-22 101
MS.gene04193.t1 AT1G30520 22.563 554 350 13 47 543 23 554 2.64e-22 101
MS.gene04193.t1 AT1G64400 25.813 492 283 20 49 469 70 550 7.38e-22 100
MS.gene04193.t1 AT4G23850 25.657 495 280 21 49 469 70 550 4.51e-20 94.7
MS.gene04193.t1 AT1G49430 23.429 525 304 24 15 455 27 537 1.98e-17 86.3
MS.gene04193.t1 AT1G30520 22.395 451 285 10 47 454 23 451 1.06e-14 77.0
MS.gene04193.t1 AT1G30520 22.395 451 285 10 47 454 23 451 1.06e-14 77.0
MS.gene04193.t1 AT2G47240 34.320 169 102 7 305 469 325 488 1.51e-13 73.9
MS.gene04193.t1 AT2G47240 34.320 169 102 7 305 469 384 547 1.60e-13 73.9
MS.gene04193.t1 AT2G47240 34.320 169 102 7 305 469 384 547 1.60e-13 73.9
MS.gene04193.t1 AT2G47240 34.320 169 102 7 305 469 384 547 1.60e-13 73.9
MS.gene04193.t1 AT3G23790 32.164 171 92 5 330 479 473 640 1.75e-13 73.9
MS.gene04193.t1 AT4G14070 31.481 162 97 4 330 480 489 647 4.43e-13 72.4
MS.gene04193.t1 AT4G14070 26.556 241 149 7 27 241 95 333 2.35e-12 70.1

Find 109 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTTTCATCCTTCCGAATT+TGG 0.115535 3.3:+48365549 MS.gene04193:CDS
GGGAGGGATCTTCCTGTTTC+AGG 0.121636 3.3:-48365589 None:intergenic
CACCATATAGTTCAGCATTT+TGG 0.132681 3.3:-48364117 None:intergenic
GCTATTTGTGACAATTAATT+TGG 0.178651 3.3:-48364395 None:intergenic
ATGGCATACTCTACAATATT+TGG 0.200376 3.3:-48364274 None:intergenic
ATTGTTCCACCTATTATTCT+TGG 0.237238 3.3:+48364936 MS.gene04193:CDS
ATTGGAAAAGTCACTACATT+AGG 0.245143 3.3:+48364768 MS.gene04193:CDS
GCATGGCACAAAGGTCATTT+TGG 0.254488 3.3:-48364641 None:intergenic
TTAACCAATCCAAGAATAAT+AGG 0.258003 3.3:-48364945 None:intergenic
GAAGATGGTGGAAAAGATTA+AGG 0.261374 3.3:+48366760 MS.gene04193:CDS
AATCTTTGTTCTACACTCTT+TGG 0.286307 3.3:+48364729 MS.gene04193:CDS
GAATATGTTGTGCAACTATT+AGG 0.306968 3.3:+48364826 MS.gene04193:CDS
CATGATTCCCAAAGCAATAA+TGG 0.317628 3.3:-48364293 None:intergenic
TCCTTTGAACATAAGTTTCC+TGG 0.330815 3.3:+48365059 MS.gene04193:CDS
AGTTCCCCTGGTGTGTTCCT+TGG 0.340867 3.3:-48365610 None:intergenic
ATTTCCCCTGCCTCTTCATC+TGG 0.345841 3.3:-48366569 None:intergenic
TCAAAGGAAGTAAGTAATTC+AGG 0.346829 3.3:-48365044 None:intergenic
GAGTATGCCATTATTGCTTT+GGG 0.348885 3.3:+48364286 MS.gene04193:CDS
AGAGTATGCCATTATTGCTT+TGG 0.356223 3.3:+48364285 MS.gene04193:CDS
GGTCTCTTGGTTTGAGAAAA+GGG 0.362079 3.3:+48364230 MS.gene04193:CDS
GTTCCCCTGGTGTGTTCCTT+GGG 0.362580 3.3:-48365609 None:intergenic
GTACCGGAATGGGCGAATAT+TGG 0.371808 3.3:-48364063 None:intergenic
AGGTCTCTTGGTTTGAGAAA+AGG 0.379071 3.3:+48364229 MS.gene04193:CDS
TCATCTCCTAGCACAATAAC+AGG 0.379300 3.3:-48364534 None:intergenic
CTCCAAAATGCTGAACTATA+TGG 0.388619 3.3:+48364115 MS.gene04193:CDS
ATAAACTTGACTTCCAAATT+CGG 0.392960 3.3:-48365562 None:intergenic
GGTTGCTCCTGCAGAGTTAG+AGG 0.394433 3.3:+48366231 MS.gene04193:intron
TCCACCTATTATTCTTGGAT+TGG 0.398159 3.3:+48364941 MS.gene04193:CDS
GGTGTAACACAAGAAATGAT+TGG 0.398343 3.3:+48364750 MS.gene04193:CDS
GCTATACCAAAATCACCTTC+TGG 0.399912 3.3:+48366710 MS.gene04193:CDS
AAGTTTATTGATCCTGAAAC+AGG 0.400350 3.3:+48365577 MS.gene04193:CDS
TAGCCAATATTCGCCCATTC+CGG 0.401957 3.3:+48364060 MS.gene04193:CDS
ACCTGAAAATGTAACATTGC+CGG 0.414752 3.3:+48364084 MS.gene04193:CDS
CATTATTGCTTTGGGAATCA+TGG 0.418941 3.3:+48364294 MS.gene04193:CDS
CTCAAAACAACACTTGCAGC+TGG 0.420863 3.3:-48366593 None:intergenic
TTGGAAAAGTCACTACATTA+GGG 0.421139 3.3:+48364769 MS.gene04193:CDS
CCTGTTGTGCCTGATGAAAA+TGG 0.423310 3.3:-48364663 None:intergenic
TCAAAGGCTTTAAGGTCTCT+TGG 0.427536 3.3:+48364217 MS.gene04193:CDS
AGAATAATAGGTGGAACAAT+AGG 0.427941 3.3:-48364933 None:intergenic
TGGTGATAAGGTTGCATTTG+TGG 0.435222 3.3:+48364135 MS.gene04193:CDS
TAGAACAAAGATTTGCTACT+AGG 0.446233 3.3:-48364719 None:intergenic
AGATCAAATTGATCAACAAT+AGG 0.448219 3.3:-48364972 None:intergenic
TTTCACATTTATGGCATCAC+TGG 0.449930 3.3:+48364804 MS.gene04193:CDS
AGTGAGAGTGGTTCACTTTG+TGG 0.451776 3.3:+48366685 MS.gene04193:CDS
AACTTCTTGAAGCCGGAGAT+CGG 0.453268 3.3:+48364586 MS.gene04193:CDS
ACTTCTTGAAGCCGGAGATC+GGG 0.460564 3.3:+48364587 MS.gene04193:CDS
TTGATACAGGTGAAAAGTCT+AGG 0.462276 3.3:+48364507 MS.gene04193:intron
ATTTCCGACGTATGTGAAGC+TGG 0.470134 3.3:-48364346 None:intergenic
CCCTGGTGTGTTCCTTGGGA+GGG 0.472351 3.3:-48365605 None:intergenic
GGGTTACCTGTTATTGTGCT+AGG 0.479109 3.3:+48364528 MS.gene04193:CDS
TGGTGCAGCAGCAGTCATAA+TGG 0.483201 3.3:-48365015 None:intergenic
CTTCTCACACAAAGTTCCCC+TGG 0.483470 3.3:-48365622 None:intergenic
GATGCTGGTTGTGTGTTCTC+AGG 0.486269 3.3:+48364316 MS.gene04193:CDS
TCCTGGTGTTCTTGTTCAAG+AGG 0.488865 3.3:+48365076 MS.gene04193:CDS
TGTGACTTCATGTGTAATCA+AGG 0.491977 3.3:-48364904 None:intergenic
ATCATTCCCTGCCCGATCTC+CGG 0.492090 3.3:-48364598 None:intergenic
TTTCTTGTAATGAGCAGCAT+TGG 0.498730 3.3:-48366663 None:intergenic
CCCCTGGTGTGTTCCTTGGG+AGG 0.500432 3.3:-48365606 None:intergenic
TGCTGAACTATATGGTGATA+AGG 0.502771 3.3:+48364123 MS.gene04193:CDS
AGATTACCAGATGAAGAGGC+AGG 0.505717 3.3:+48366563 MS.gene04193:intron
AAATAGCTCAAGCCATGAAA+AGG 0.506415 3.3:+48364411 MS.gene04193:CDS
TTGTTGAAGATGCTATGAAC+TGG 0.506560 3.3:+48364559 MS.gene04193:CDS
AAAATAGCCTCTAACTCTGC+AGG 0.509463 3.3:-48366238 None:intergenic
GTGCAACTATTAGGAACAAA+GGG 0.513378 3.3:+48364835 MS.gene04193:CDS
GTGGATGCTGAGAGTGGAAA+AGG 0.515304 3.3:+48364154 MS.gene04193:CDS
GCTTTGGGAATCATGGATGC+TGG 0.516352 3.3:+48364301 MS.gene04193:CDS
CTTGAAGCCGGAGATCGGGC+AGG 0.519508 3.3:+48364591 MS.gene04193:CDS
TAACTTATAGTGAGGTTGTG+AGG 0.520474 3.3:+48364179 MS.gene04193:CDS
CAAAGGGAAAGTAGTGGTGA+TGG 0.526343 3.3:+48364851 MS.gene04193:CDS
ACCAGGAAACTTATGTTCAA+AGG 0.540093 3.3:-48365060 None:intergenic
AACTTATAGTGAGGTTGTGA+GGG 0.541554 3.3:+48364180 MS.gene04193:CDS
TGGCTTCACACTGGTGACAT+AGG 0.543428 3.3:+48365878 MS.gene04193:CDS
AAGGCTTGTCAAAGAGAAGA+TGG 0.550369 3.3:+48366745 MS.gene04193:CDS
TTACAGATTACCAGATGAAG+AGG 0.552310 3.3:+48366559 MS.gene04193:intron
TGGAACAAACTTCTTGAAGC+CGG 0.560308 3.3:+48364579 MS.gene04193:CDS
AAAGGGAAAGTAGTGGTGAT+GGG 0.563703 3.3:+48364852 MS.gene04193:CDS
GGCACAACAGGAATGTCAAA+GGG 0.563766 3.3:+48364675 MS.gene04193:CDS
TCAAAACAACACTTGCAGCT+GGG 0.565486 3.3:-48366592 None:intergenic
GGCAGGGAATGATTCAATGA+AGG 0.571367 3.3:+48364608 MS.gene04193:CDS
AGGCACAACAGGAATGTCAA+AGG 0.574039 3.3:+48364674 MS.gene04193:CDS
TCCCTCCCAAGGAACACACC+AGG 0.574638 3.3:+48365604 MS.gene04193:CDS
AAGGAGTTGATTAAATACAA+AGG 0.576483 3.3:+48365944 MS.gene04193:CDS
ACCTCTTGAACAAGAACACC+AGG 0.581302 3.3:-48365077 None:intergenic
GATTACCTTGCATTACACAT+TGG 0.583044 3.3:-48365645 None:intergenic
ACTTGACTTCCAAATTCGGA+AGG 0.585552 3.3:-48365558 None:intergenic
TGTGCAACTATTAGGAACAA+AGG 0.585600 3.3:+48364834 MS.gene04193:CDS
GATTACCAGATGAAGAGGCA+GGG 0.588955 3.3:+48366564 MS.gene04193:intron
ACTAGGTTATTATAAACAAG+TGG 0.592598 3.3:+48365823 MS.gene04193:intron
ATGAAAATGGCATGGCACAA+AGG 0.593972 3.3:-48364650 None:intergenic
TGATACAGGTGAAAAGTCTA+GGG 0.594118 3.3:+48364508 MS.gene04193:intron
CCCTCCCAAGGAACACACCA+GGG 0.599440 3.3:+48365605 MS.gene04193:CDS
TTGAAGCCGGAGATCGGGCA+GGG 0.599597 3.3:+48364592 MS.gene04193:CDS
ATTACCAGATGAAGAGGCAG+GGG 0.606768 3.3:+48366565 MS.gene04193:CDS
AAAAGGAGTAACTTATAGTG+AGG 0.610280 3.3:+48364171 MS.gene04193:CDS
GTAAGTAATTCAGGTGCAAG+TGG 0.616501 3.3:-48365035 None:intergenic
CTTCTGGTAAAATAATGAGA+AGG 0.618252 3.3:+48366726 MS.gene04193:CDS
GAATGGGCGAATATTGGCTA+CGG 0.618752 3.3:-48364057 None:intergenic
AACAGCTGCATCTTCAACTG+AGG 0.622182 3.3:-48366272 None:intergenic
GCATTTGTGGATGCTGAGAG+TGG 0.629816 3.3:+48364148 MS.gene04193:CDS
ACCAATCCAAGAATAATAGG+TGG 0.632007 3.3:-48364942 None:intergenic
TAGGAACAAAGGGAAAGTAG+TGG 0.635216 3.3:+48364845 MS.gene04193:CDS
AAACAGGAAGATCCCTCCCA+AGG 0.648638 3.3:+48365593 MS.gene04193:CDS
TCATTACAAGAAAGTGAGAG+TGG 0.654711 3.3:+48366673 MS.gene04193:CDS
TAATCCAGCTTCACATACGT+CGG 0.679378 3.3:+48364342 MS.gene04193:CDS
CCTCCCAAGGAACACACCAG+GGG 0.693772 3.3:+48365606 MS.gene04193:CDS
AGAAGCCAATGTGTAATGCA+AGG 0.701599 3.3:+48365640 MS.gene04193:CDS
GCTTGTCAAAGAGAAGATGG+TGG 0.703710 3.3:+48366748 MS.gene04193:CDS
TGTGCCTGATGAAAATGGCA+TGG 0.715816 3.3:-48364658 None:intergenic
AGGGTGTGATGCTTACACAC+AGG 0.727103 3.3:+48364694 MS.gene04193:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTACCAATTTATCAAATAA+AGG + chr3.3:48366098-48366117 MS.gene04193:intron 15.0%
!!! AAAAATTGTTAGATTTTGTT+AGG - chr3.3:48366796-48366815 None:intergenic 15.0%
!!! AAAATTGTTAGATTTTGTTA+GGG - chr3.3:48366795-48366814 None:intergenic 15.0%
!!! TTAACCTTTATTTGATAAAT+TGG - chr3.3:48366105-48366124 None:intergenic 15.0%
!! TTATAGATGCATATTAATCT+GGG - chr3.3:48366146-48366165 None:intergenic 20.0%
!! TTATCAAATAAAGGTTAACT+TGG + chr3.3:48366107-48366126 MS.gene04193:intron 20.0%
!!! AAGTTCTGTTTTATCAAATT+TGG + chr3.3:48366055-48366074 MS.gene04193:intron 20.0%
!!! TTCTACAAAATTTTCTATGT+AGG - chr3.3:48364020-48364039 None:intergenic 20.0%
!!! TTTTATTGTTGATCGTATTA+AGG + chr3.3:48365925-48365944 MS.gene04193:CDS 20.0%
!!! TTTTTTACATTATGTGTTTC+TGG + chr3.3:48365277-48365296 MS.gene04193:intron 20.0%
!!! TTTTTTTAGACAAATGTTAG+TGG + chr3.3:48365702-48365721 MS.gene04193:intron 20.0%
! AATAACAAAAATGATTGTCG+CGG - chr3.3:48366317-48366336 None:intergenic 25.0%
! AATATGCATCTATAAGTCTA+AGG + chr3.3:48366151-48366170 MS.gene04193:intron 25.0%
! AATGTTAGTGGTTAATTGTT+AGG + chr3.3:48365714-48365733 MS.gene04193:intron 25.0%
! ACATATGCACAAAAATTATC+TGG + chr3.3:48365505-48365524 MS.gene04193:CDS 25.0%
! ACTAGGTTATTATAAACAAG+TGG + chr3.3:48365823-48365842 MS.gene04193:intron 25.0%
! AGATCAAATTGATCAACAAT+AGG - chr3.3:48364975-48364994 None:intergenic 25.0%
! ATAAACTTGACTTCCAAATT+CGG - chr3.3:48365565-48365584 None:intergenic 25.0%
! ATCAACATTGTATCAAATCA+AGG - chr3.3:48364492-48364511 None:intergenic 25.0%
! ATGCCATAAATGTGAAAAAA+GGG - chr3.3:48364801-48364820 None:intergenic 25.0%
! ATGGAACAAAACATTATTTG+TGG + chr3.3:48366006-48366025 MS.gene04193:intron 25.0%
! ATGTAAAAAAAATCTAACGC+AGG - chr3.3:48365267-48365286 None:intergenic 25.0%
! ATGTTAGTGGTTAATTGTTA+GGG + chr3.3:48365715-48365734 MS.gene04193:intron 25.0%
! ATTTGATACAATGTTGATAC+AGG + chr3.3:48364494-48364513 MS.gene04193:intron 25.0%
! CACTTAAGAAACACATAAAA+GGG - chr3.3:48365430-48365449 None:intergenic 25.0%
! CTTATAGATGCATATTAATC+TGG - chr3.3:48366147-48366166 None:intergenic 25.0%
! GCTATTTGTGACAATTAATT+TGG - chr3.3:48364398-48364417 None:intergenic 25.0%
! GTTACATGTTTAATGTTACA+TGG + chr3.3:48365987-48366006 MS.gene04193:intron 25.0%
! TGAACTAACAATACTATAGA+AGG - chr3.3:48365115-48365134 None:intergenic 25.0%
! TGTAAAAAAAATCTAACGCA+GGG - chr3.3:48365266-48365285 None:intergenic 25.0%
! TGTAACATCAATAACTTACT+TGG - chr3.3:48365973-48365992 None:intergenic 25.0%
! TTAACCAATCCAAGAATAAT+AGG - chr3.3:48364948-48364967 None:intergenic 25.0%
!! AAGGAGTTGATTAAATACAA+AGG + chr3.3:48365944-48365963 MS.gene04193:CDS 25.0%
!! AGTCAAACATTTTTAATCGA+CGG + chr3.3:48366469-48366488 MS.gene04193:intron 25.0%
!! ATAGATTTTCAAAGGCTTTA+AGG + chr3.3:48364209-48364228 MS.gene04193:CDS 25.0%
!! ATGATTCATCATCATTTTGA+AGG - chr3.3:48364442-48364461 None:intergenic 25.0%
!! GGATATACATAGATTTTCAA+AGG + chr3.3:48364201-48364220 MS.gene04193:CDS 25.0%
!! GTTTAATTTAAATATACCGC+CGG + chr3.3:48366376-48366395 MS.gene04193:intron 25.0%
!! TGGAAGTACACATAAAAATT+CGG + chr3.3:48365525-48365544 MS.gene04193:CDS 25.0%
!!! AAACTCTTTTTGTTTCTACT+AGG + chr3.3:48365806-48365825 MS.gene04193:intron 25.0%
!!! AACTAATTTTAGCCTATACT+TGG - chr3.3:48366187-48366206 None:intergenic 25.0%
!!! ACTAATTTTAGCCTATACTT+GGG - chr3.3:48366186-48366205 None:intergenic 25.0%
!!! ATTCCCTTTTTTCACATTTA+TGG + chr3.3:48364795-48364814 MS.gene04193:CDS 25.0%
!!! GTTTTTTCTTTTTTGAATGC+AGG + chr3.3:48366210-48366229 MS.gene04193:intron 25.0%
!!! TTTTTTACAGTATGTGTTTC+TGG + chr3.3:48365155-48365174 MS.gene04193:intron 25.0%
AAAAGGAGTAACTTATAGTG+AGG + chr3.3:48364171-48364190 MS.gene04193:CDS 30.0%
AAGTTTATTGATCCTGAAAC+AGG + chr3.3:48365577-48365596 MS.gene04193:CDS 30.0%
AATCTTTGTTCTACACTCTT+TGG + chr3.3:48364729-48364748 MS.gene04193:CDS 30.0%
AGAATAATAGGTGGAACAAT+AGG - chr3.3:48364936-48364955 None:intergenic 30.0%
AGACTATTGACAAAAATGGA+TGG + chr3.3:48365858-48365877 MS.gene04193:CDS 30.0%
AGTACACATAAAAATTCGGT+CGG + chr3.3:48365529-48365548 MS.gene04193:CDS 30.0%
ATAACAAAAATGATTGTCGC+GGG - chr3.3:48366316-48366335 None:intergenic 30.0%
ATATGATTGATTGTGATCTG+TGG - chr3.3:48366421-48366440 None:intergenic 30.0%
ATCTTAAATCCACAATGAAG+AGG - chr3.3:48366353-48366372 None:intergenic 30.0%
ATGGCATACTCTACAATATT+TGG - chr3.3:48364277-48364296 None:intergenic 30.0%
ATTGGAAAAGTCACTACATT+AGG + chr3.3:48364768-48364787 MS.gene04193:CDS 30.0%
ATTGTTCCACCTATTATTCT+TGG + chr3.3:48364936-48364955 MS.gene04193:CDS 30.0%
CTTCTGGTAAAATAATGAGA+AGG + chr3.3:48366726-48366745 MS.gene04193:CDS 30.0%
GAATATGTTGTGCAACTATT+AGG + chr3.3:48364826-48364845 MS.gene04193:CDS 30.0%
GATGCCATAAATGTGAAAAA+AGG - chr3.3:48364802-48364821 None:intergenic 30.0%
GCACTTAAGAAACACATAAA+AGG - chr3.3:48365431-48365450 None:intergenic 30.0%
TCAAAGGAAGTAAGTAATTC+AGG - chr3.3:48365047-48365066 None:intergenic 30.0%
TGTTAGTGGTTAATTGTTAG+GGG + chr3.3:48365716-48365735 MS.gene04193:intron 30.0%
TTAATGTTACCTCTTCATTG+TGG + chr3.3:48366341-48366360 MS.gene04193:intron 30.0%
TTGGAAAAGTCACTACATTA+GGG + chr3.3:48364769-48364788 MS.gene04193:CDS 30.0%
! ATATGTTGATCCTTTTACAC+CGG - chr3.3:48366398-48366417 None:intergenic 30.0%
! ATTTTACCAGAAGGTGATTT+TGG - chr3.3:48366719-48366738 None:intergenic 30.0%
!! AAATCAAGGAGTATCGAAAA+AGG - chr3.3:48364478-48364497 None:intergenic 30.0%
!! CTTCTCATTATTTTACCAGA+AGG - chr3.3:48366728-48366747 None:intergenic 30.0%
!! TAGAACAAAGATTTGCTACT+AGG - chr3.3:48364722-48364741 None:intergenic 30.0%
!! TTACATTATGTGTTTCTGGA+GGG + chr3.3:48365281-48365300 MS.gene04193:intron 30.0%
!! TTTACATTATGTGTTTCTGG+AGG + chr3.3:48365280-48365299 MS.gene04193:intron 30.0%
!!! AATGTTACATTTTCAGGTAC+CGG - chr3.3:48364082-48364101 None:intergenic 30.0%
!!! GTGCTAATTTTTTCTTTTGC+AGG + chr3.3:48365447-48365466 MS.gene04193:intron 30.0%
!!! TTTTTTCTTTTGCAGGCATA+TGG + chr3.3:48365454-48365473 MS.gene04193:intron 30.0%
AAAAAATCTAACGCAGGGTT+CGG - chr3.3:48365261-48365280 None:intergenic 35.0%
AAATAGCTCAAGCCATGAAA+AGG + chr3.3:48364411-48364430 MS.gene04193:CDS 35.0%
AACTTATAGTGAGGTTGTGA+GGG + chr3.3:48364180-48364199 MS.gene04193:CDS 35.0%
ACCAATCCAAGAATAATAGG+TGG - chr3.3:48364945-48364964 None:intergenic 35.0%
ACCAGGAAACTTATGTTCAA+AGG - chr3.3:48365063-48365082 None:intergenic 35.0%
ACCTGAAAATGTAACATTGC+CGG + chr3.3:48364084-48364103 MS.gene04193:CDS 35.0%
AGAAACACATAAAAGGGCAA+AGG - chr3.3:48365424-48365443 None:intergenic 35.0%
AGAGTATGCCATTATTGCTT+TGG + chr3.3:48364285-48364304 MS.gene04193:CDS 35.0%
ATTATTAACGGCAATACCAC+TGG + chr3.3:48365748-48365767 MS.gene04193:intron 35.0%
CATGATTCCCAAAGCAATAA+TGG - chr3.3:48364296-48364315 None:intergenic 35.0%
CATTTATCATAAGTCTCCGA+AGG - chr3.3:48365780-48365799 None:intergenic 35.0%
CTCCAAAATGCTGAACTATA+TGG + chr3.3:48364115-48364134 MS.gene04193:CDS 35.0%
GAAACACATAAAAGGGCAAA+GGG - chr3.3:48365423-48365442 None:intergenic 35.0%
GAGTATGCCATTATTGCTTT+GGG + chr3.3:48364286-48364305 MS.gene04193:CDS 35.0%
GATTACCTTGCATTACACAT+TGG - chr3.3:48365648-48365667 None:intergenic 35.0%
GCTCAGACTATTGACAAAAA+TGG + chr3.3:48365854-48365873 MS.gene04193:CDS 35.0%
GGGAAGGCGAAAATTATTAA+CGG + chr3.3:48365736-48365755 MS.gene04193:intron 35.0%
GGTGTAACACAAGAAATGAT+TGG + chr3.3:48364750-48364769 MS.gene04193:CDS 35.0%
GTGCAACTATTAGGAACAAA+GGG + chr3.3:48364835-48364854 MS.gene04193:CDS 35.0%
TAACTTATAGTGAGGTTGTG+AGG + chr3.3:48364179-48364198 MS.gene04193:CDS 35.0%
TCATTACAAGAAAGTGAGAG+TGG + chr3.3:48366673-48366692 MS.gene04193:CDS 35.0%
TCCACCTATTATTCTTGGAT+TGG + chr3.3:48364941-48364960 MS.gene04193:CDS 35.0%
TCCTTTGAACATAAGTTTCC+TGG + chr3.3:48365059-48365078 MS.gene04193:CDS 35.0%
TGATACAGGTGAAAAGTCTA+GGG + chr3.3:48364508-48364527 MS.gene04193:intron 35.0%
TGCTGAACTATATGGTGATA+AGG + chr3.3:48364123-48364142 MS.gene04193:CDS 35.0%
TGTGACTTCATGTGTAATCA+AGG - chr3.3:48364907-48364926 None:intergenic 35.0%
TGTGCAACTATTAGGAACAA+AGG + chr3.3:48364834-48364853 MS.gene04193:CDS 35.0%
TTACAGATTACCAGATGAAG+AGG + chr3.3:48366559-48366578 MS.gene04193:intron 35.0%
TTGATACAGGTGAAAAGTCT+AGG + chr3.3:48364507-48364526 MS.gene04193:intron 35.0%
TTGTTGAAGATGCTATGAAC+TGG + chr3.3:48364559-48364578 MS.gene04193:CDS 35.0%
TTTCACATTTATGGCATCAC+TGG + chr3.3:48364804-48364823 MS.gene04193:CDS 35.0%
TTTCTTGTAATGAGCAGCAT+TGG - chr3.3:48366666-48366685 None:intergenic 35.0%
! CACCATATAGTTCAGCATTT+TGG - chr3.3:48364120-48364139 None:intergenic 35.0%
! TTGAAGGAGTTACCTTTTCA+TGG - chr3.3:48364426-48364445 None:intergenic 35.0%
!! CAAAGGTCATTTTGGTGAAT+TGG - chr3.3:48364636-48364655 None:intergenic 35.0%
!! CATTATTGCTTTGGGAATCA+TGG + chr3.3:48364294-48364313 MS.gene04193:CDS 35.0%
!! GAAGATGGTGGAAAAGATTA+AGG + chr3.3:48366760-48366779 MS.gene04193:CDS 35.0%
!! TACATTATGTGTTTCTGGAG+GGG + chr3.3:48365282-48365301 MS.gene04193:intron 35.0%
!! TTACAGTATGTGTTTCTGGA+GGG + chr3.3:48365159-48365178 MS.gene04193:intron 35.0%
!! TTTACAGTATGTGTTTCTGG+AGG + chr3.3:48365158-48365177 MS.gene04193:intron 35.0%
AAAAATCTAACGCAGGGTTC+GGG - chr3.3:48365260-48365279 None:intergenic 40.0%
AAAAATGGATGGCTTCACAC+TGG + chr3.3:48365869-48365888 MS.gene04193:CDS 40.0%
AAAATAGCCTCTAACTCTGC+AGG - chr3.3:48366241-48366260 None:intergenic 40.0%
AAAGGGAAAGTAGTGGTGAT+GGG + chr3.3:48364852-48364871 MS.gene04193:CDS 40.0%
AAATATACCGCCGGTGTAAA+AGG + chr3.3:48366385-48366404 MS.gene04193:intron 40.0%
AAGGCTTGTCAAAGAGAAGA+TGG + chr3.3:48366745-48366764 MS.gene04193:CDS 40.0%
ACTTGACTTCCAAATTCGGA+AGG - chr3.3:48365561-48365580 None:intergenic 40.0%
AGAAGCCAATGTGTAATGCA+AGG + chr3.3:48365640-48365659 MS.gene04193:CDS 40.0%
AGTGGTTAATTGTTAGGGGA+AGG + chr3.3:48365720-48365739 MS.gene04193:intron 40.0%
ATGAAAATGGCATGGCACAA+AGG - chr3.3:48364653-48364672 None:intergenic 40.0%
GCTATACCAAAATCACCTTC+TGG + chr3.3:48366710-48366729 MS.gene04193:CDS 40.0%
GTAAGTAATTCAGGTGCAAG+TGG - chr3.3:48365038-48365057 None:intergenic 40.0%
GTCATGTGACTGAAAGGAAA+CGG + chr3.3:48365211-48365230 MS.gene04193:intron 40.0%
GTTGTTGTCATGTGACTGAA+AGG + chr3.3:48365205-48365224 MS.gene04193:intron 40.0%
TAAAAGGGCAAAGGGAAATC+TGG - chr3.3:48365415-48365434 None:intergenic 40.0%
TAATCCAGCTTCACATACGT+CGG + chr3.3:48364342-48364361 MS.gene04193:CDS 40.0%
TAGGAACAAAGGGAAAGTAG+TGG + chr3.3:48364845-48364864 MS.gene04193:CDS 40.0%
TCAAAACAACACTTGCAGCT+GGG - chr3.3:48366595-48366614 None:intergenic 40.0%
TCATCTCCTAGCACAATAAC+AGG - chr3.3:48364537-48364556 None:intergenic 40.0%
TCATGTGACTGAAAGGAAAC+GGG + chr3.3:48365212-48365231 MS.gene04193:intron 40.0%
TGGAACAAACTTCTTGAAGC+CGG + chr3.3:48364579-48364598 MS.gene04193:CDS 40.0%
TGGTGATAAGGTTGCATTTG+TGG + chr3.3:48364135-48364154 MS.gene04193:CDS 40.0%
! AAGGAAACGGGTTCAAGTAT+TGG + chr3.3:48365224-48365243 MS.gene04193:intron 40.0%
! AGGTCTCTTGGTTTGAGAAA+AGG + chr3.3:48364229-48364248 MS.gene04193:CDS 40.0%
! GGTCTCTTGGTTTGAGAAAA+GGG + chr3.3:48364230-48364249 MS.gene04193:CDS 40.0%
! TCCGGCAATGTTACATTTTC+AGG - chr3.3:48364088-48364107 None:intergenic 40.0%
!! TACAGTATGTGTTTCTGGAG+GGG + chr3.3:48365160-48365179 MS.gene04193:intron 40.0%
!! TCAAAGGCTTTAAGGTCTCT+TGG + chr3.3:48364217-48364236 MS.gene04193:CDS 40.0%
!!! ACATTTTCAGGTACCGGAAT+GGG - chr3.3:48364076-48364095 None:intergenic 40.0%
!!! GCAAGTGTTGTTTTGAGCAA+AGG + chr3.3:48366599-48366618 MS.gene04193:CDS 40.0%
!!! GCATTTTGGAGCACAAATTC+CGG - chr3.3:48364106-48364125 None:intergenic 40.0%
!!! TACATTTTCAGGTACCGGAA+TGG - chr3.3:48364077-48364096 None:intergenic 40.0%
AACAGCTGCATCTTCAACTG+AGG - chr3.3:48366275-48366294 None:intergenic 45.0%
AACTTCTTGAAGCCGGAGAT+CGG + chr3.3:48364586-48364605 MS.gene04193:CDS 45.0%
ACCTCTTGAACAAGAACACC+AGG - chr3.3:48365080-48365099 None:intergenic 45.0%
AGATTACCAGATGAAGAGGC+AGG + chr3.3:48366563-48366582 MS.gene04193:intron 45.0%
AGGCACAACAGGAATGTCAA+AGG + chr3.3:48364674-48364693 MS.gene04193:CDS 45.0%
AGTGAGAGTGGTTCACTTTG+TGG + chr3.3:48366685-48366704 MS.gene04193:CDS 45.0%
ATACCACTGGACTACACCTT+CGG + chr3.3:48365761-48365780 MS.gene04193:intron 45.0%
ATTACCAGATGAAGAGGCAG+GGG + chr3.3:48366565-48366584 MS.gene04193:CDS 45.0%
ATTTCCGACGTATGTGAAGC+TGG - chr3.3:48364349-48364368 None:intergenic 45.0%
CAAAGGGAAAGTAGTGGTGA+TGG + chr3.3:48364851-48364870 MS.gene04193:CDS 45.0%
CCTGTTGTGCCTGATGAAAA+TGG - chr3.3:48364666-48364685 None:intergenic 45.0%
CGGTTTCATCCTTCCGAATT+TGG + chr3.3:48365549-48365568 MS.gene04193:CDS 45.0%
CTCAAAACAACACTTGCAGC+TGG - chr3.3:48366596-48366615 None:intergenic 45.0%
GATTACCAGATGAAGAGGCA+GGG + chr3.3:48366564-48366583 MS.gene04193:intron 45.0%
GCTTGTCAAAGAGAAGATGG+TGG + chr3.3:48366748-48366767 MS.gene04193:CDS 45.0%
GGCACAACAGGAATGTCAAA+GGG + chr3.3:48364675-48364694 MS.gene04193:CDS 45.0%
GGCAGGGAATGATTCAATGA+AGG + chr3.3:48364608-48364627 MS.gene04193:CDS 45.0%
GGGTTACCTGTTATTGTGCT+AGG + chr3.3:48364528-48364547 MS.gene04193:CDS 45.0%
TAGCCAATATTCGCCCATTC+CGG + chr3.3:48364060-48364079 MS.gene04193:CDS 45.0%
TCCTGGTGTTCTTGTTCAAG+AGG + chr3.3:48365076-48365095 MS.gene04193:CDS 45.0%
TGTGCCTGATGAAAATGGCA+TGG - chr3.3:48364661-48364680 None:intergenic 45.0%
TTAGCCTATACTTGGGCAGA+AGG - chr3.3:48366179-48366198 None:intergenic 45.0%
! CCATTTTCATCAGGCACAAC+AGG + chr3.3:48364663-48364682 MS.gene04193:CDS 45.0%
! GAATGGGCGAATATTGGCTA+CGG - chr3.3:48364060-48364079 None:intergenic 45.0%
! GCATGGCACAAAGGTCATTT+TGG - chr3.3:48364644-48364663 None:intergenic 45.0%
! TGTTGATCCTTTTACACCGG+CGG - chr3.3:48366395-48366414 None:intergenic 45.0%
!!! TGTGCCATGCCATTTTCATC+AGG + chr3.3:48364654-48364673 MS.gene04193:CDS 45.0%
AAAAATGATTGTCGCGGGCG+CGG - chr3.3:48366311-48366330 None:intergenic 50.0%
AAACAGGAAGATCCCTCCCA+AGG + chr3.3:48365593-48365612 MS.gene04193:CDS 50.0%
ACTTCTTGAAGCCGGAGATC+GGG + chr3.3:48364587-48364606 MS.gene04193:CDS 50.0%
AGGGTGTGATGCTTACACAC+AGG + chr3.3:48364694-48364713 MS.gene04193:CDS 50.0%
ATTTCCCCTGCCTCTTCATC+TGG - chr3.3:48366572-48366591 None:intergenic 50.0%
CAACTTTACCAGTTGCGCCA+AGG - chr3.3:48365189-48365208 None:intergenic 50.0%
CTTCTCACACAAAGTTCCCC+TGG - chr3.3:48365625-48365644 None:intergenic 50.0%
GCATTTGTGGATGCTGAGAG+TGG + chr3.3:48364148-48364167 MS.gene04193:CDS 50.0%
GGAACCTTCTGCCCAAGTAT+AGG + chr3.3:48366172-48366191 MS.gene04193:intron 50.0%
GTACCGGAATGGGCGAATAT+TGG - chr3.3:48364066-48364085 None:intergenic 50.0%
GTGGATGCTGAGAGTGGAAA+AGG + chr3.3:48364154-48364173 MS.gene04193:CDS 50.0%
TGGCTTCACACTGGTGACAT+AGG + chr3.3:48365878-48365897 MS.gene04193:CDS 50.0%
TGGTGCAGCAGCAGTCATAA+TGG - chr3.3:48365018-48365037 None:intergenic 50.0%
! CAACTTTTCCAGTTGCGCCA+AGG - chr3.3:48365311-48365330 None:intergenic 50.0%
! GATGCTGGTTGTGTGTTCTC+AGG + chr3.3:48364316-48364335 MS.gene04193:CDS 50.0%
!! GCTTTGGGAATCATGGATGC+TGG + chr3.3:48364301-48364320 MS.gene04193:CDS 50.0%
!! TGTTTCTGGAGGGGTAACCT+TGG + chr3.3:48365169-48365188 MS.gene04193:intron 50.0%
ACTAAAGCTCCCGCATACGC+AGG - chr3.3:48365389-48365408 None:intergenic 55.0%
AGTTCCCCTGGTGTGTTCCT+TGG - chr3.3:48365613-48365632 None:intergenic 55.0%
ATCATTCCCTGCCCGATCTC+CGG - chr3.3:48364601-48364620 None:intergenic 55.0%
ATCTAACGCAGGGTTCGGGA+AGG - chr3.3:48365256-48365275 None:intergenic 55.0%
CTAAAGCTCCCGCATACGCA+GGG - chr3.3:48365388-48365407 None:intergenic 55.0%
GGGAGGGATCTTCCTGTTTC+AGG - chr3.3:48365592-48365611 None:intergenic 55.0%
GGTTGCTCCTGCAGAGTTAG+AGG + chr3.3:48366231-48366250 MS.gene04193:intron 55.0%
GTTCCCCTGGTGTGTTCCTT+GGG - chr3.3:48365612-48365631 None:intergenic 55.0%
! TCTCCGAAGGTGTAGTCCAG+TGG - chr3.3:48365767-48365786 None:intergenic 55.0%
AGGGGTAACCTTGGCGCAAC+TGG + chr3.3:48365178-48365197 MS.gene04193:intron 60.0%
CCCTCCCAAGGAACACACCA+GGG + chr3.3:48365605-48365624 MS.gene04193:CDS 60.0%
CCCTGGTGTGTTCCTTGGGA+GGG - chr3.3:48365608-48365627 None:intergenic 60.0%
CCTCCCAAGGAACACACCAG+GGG + chr3.3:48365606-48365625 MS.gene04193:CDS 60.0%
CTTCCCGAACCCTGCGTATG+CGG + chr3.3:48365376-48365395 MS.gene04193:intron 60.0%
TCCCTCCCAAGGAACACACC+AGG + chr3.3:48365604-48365623 MS.gene04193:CDS 60.0%
TTCCCGAACCCTGCGTATGC+GGG + chr3.3:48365377-48365396 MS.gene04193:intron 60.0%
TTGAAGCCGGAGATCGGGCA+GGG + chr3.3:48364592-48364611 MS.gene04193:CDS 60.0%
CCCCTGGTGTGTTCCTTGGG+AGG - chr3.3:48365609-48365628 None:intergenic 65.0%
CGCATACGCAGGGTTCGGGA+AGG - chr3.3:48365378-48365397 None:intergenic 65.0%
CTCCCGCATACGCAGGGTTC+GGG - chr3.3:48365382-48365401 None:intergenic 65.0%
CTTGAAGCCGGAGATCGGGC+AGG + chr3.3:48364591-48364610 MS.gene04193:CDS 65.0%
GCTCCCGCATACGCAGGGTT+CGG - chr3.3:48365383-48365402 None:intergenic 65.0%
GTCGCGGGCGCGGTTACTTA+CGG - chr3.3:48366301-48366320 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 48364010 48366817 48364010 ID=MS.gene04193
chr3.3 mRNA 48364010 48366817 48364010 ID=MS.gene04193.t1;Parent=MS.gene04193
chr3.3 exon 48364010 48364432 48364010 ID=MS.gene04193.t1.exon1;Parent=MS.gene04193.t1
chr3.3 CDS 48364010 48364432 48364010 ID=cds.MS.gene04193.t1;Parent=MS.gene04193.t1
chr3.3 exon 48364516 48365097 48364516 ID=MS.gene04193.t1.exon2;Parent=MS.gene04193.t1
chr3.3 CDS 48364516 48365097 48364516 ID=cds.MS.gene04193.t1;Parent=MS.gene04193.t1
chr3.3 exon 48365469 48365661 48365469 ID=MS.gene04193.t1.exon3;Parent=MS.gene04193.t1
chr3.3 CDS 48365469 48365661 48365469 ID=cds.MS.gene04193.t1;Parent=MS.gene04193.t1
chr3.3 exon 48365828 48365973 48365828 ID=MS.gene04193.t1.exon4;Parent=MS.gene04193.t1
chr3.3 CDS 48365828 48365973 48365828 ID=cds.MS.gene04193.t1;Parent=MS.gene04193.t1
chr3.3 exon 48366232 48366299 48366232 ID=MS.gene04193.t1.exon5;Parent=MS.gene04193.t1
chr3.3 CDS 48366232 48366299 48366232 ID=cds.MS.gene04193.t1;Parent=MS.gene04193.t1
chr3.3 exon 48366565 48366817 48366565 ID=MS.gene04193.t1.exon6;Parent=MS.gene04193.t1
chr3.3 CDS 48366565 48366817 48366565 ID=cds.MS.gene04193.t1;Parent=MS.gene04193.t1
Gene Sequence

>MS.gene04193

ATGGGAGCCTACATAGAAAATTTTGTAGAAGAGAATGAACACATTTTCCGTAGCCAATATTCGCCCATTCCGGTACCTGAAAATGTAACATTGCCGGAATTTGTGCTCCAAAATGCTGAACTATATGGTGATAAGGTTGCATTTGTGGATGCTGAGAGTGGAAAAGGAGTAACTTATAGTGAGGTTGTGAGGGATATACATAGATTTTCAAAGGCTTTAAGGTCTCTTGGTTTGAGAAAAGGGAATGTTGTGATTGTTGTACTTCCAAATATTGTAGAGTATGCCATTATTGCTTTGGGAATCATGGATGCTGGTTGTGTGTTCTCAGGAGCTAATCCAGCTTCACATACGTCGGAAATTAAGAAACAAGTTGAGTCTGCTGATGCCAAATTAATTGTCACAAATAGCTCAAGCCATGAAAAGGTAACTCCTTCAAAATGATGATGAATCATGTTTTTTCTTATGCCTTTTTCGATACTCCTTGATTTGATACAATGTTGATACAGGTGAAAAGTCTAGGGTTACCTGTTATTGTGCTAGGAGATGAACTTGTTGAAGATGCTATGAACTGGAACAAACTTCTTGAAGCCGGAGATCGGGCAGGGAATGATTCAATGAAGGAACCAATTCACCAAAATGACCTTTGTGCCATGCCATTTTCATCAGGCACAACAGGAATGTCAAAGGGTGTGATGCTTACACACAGGAACCTAGTAGCAAATCTTTGTTCTACACTCTTTGGTGTAACACAAGAAATGATTGGAAAAGTCACTACATTAGGGTTAATTCCCTTTTTTCACATTTATGGCATCACTGGAATATGTTGTGCAACTATTAGGAACAAAGGGAAAGTAGTGGTGATGGGAAAATATGATTTGAAAACATTTTTGAATGCCTTGATTACACATGAAGTCACATTTGCACCTATTGTTCCACCTATTATTCTTGGATTGGTTAAAAATCCTATTGTTGATCAATTTGATCTTAGTAAGCTTAAACTTCAAGCCATTATGACTGCTGCTGCACCACTTGCACCTGAATTACTTACTTCCTTTGAACATAAGTTTCCTGGTGTTCTTGTTCAAGAGGTTATCACTTTCATCCTTCTATAGTATTGTTAGTTCAATTAAAGACTTTTTTAGATTTTTTTTACAGTATGTGTTTCTGGAGGGGTAACCTTGGCGCAACTGGTAAAGTTGTTGTCATGTGACTGAAAGGAAACGGGTTCAAGTATTGGAAACAGCCTTCCCGAACCCTGCGTTAGATTTTTTTTACATTATGTGTTTCTGGAGGGGTAACCTTGGCGCAACTGGAAAAGTTGTTGTCATGTGACTGAAAGGAAACGGGTTCAAGTATTGGAAACAGCCTTCCCGAACCCTGCGTATGCGGGAGCTTTAGTGCACCAGATTTCCCTTTGCCCTTTTATGTGTTTCTTAAGTGCTAATTTTTTCTTTTGCAGGCATATGGACTAACTGAGCATAGCTGCATCACACTTACATATGCACAAAAATTATCTGGAAGTACACATAAAAATTCGGTCGGTTTCATCCTTCCGAATTTGGAAGTCAAGTTTATTGATCCTGAAACAGGAAGATCCCTCCCAAGGAACACACCAGGGGAACTTTGTGTGAGAAGCCAATGTGTAATGCAAGGTAATCTATGACTATGAGTAAAATTATTTATGATAATATATTTTTTTAGACAAATGTTAGTGGTTAATTGTTAGGGGAAGGCGAAAATTATTAACGGCAATACCACTGGACTACACCTTCGGAGACTTATGATAAATGTACTATAAACTCTTTTTGTTTCTACTAGGTTATTATAAACAAGTGGATGAGACTGCTCAGACTATTGACAAAAATGGATGGCTTCACACTGGTGACATAGGATTCATAGATGATGAAGAAAATGTTTTTATTGTTGATCGTATTAAGGAGTTGATTAAATACAAAGGCTTCCAAGTAAGTTATTGATGTTACATGTTTAATGTTACATGGAACAAAACATTATTTGTGGAACATAATTGCAATGTCTTAGCTAGCAAGTTCTGTTTTATCAAATTTGGACAACATTTAACGTTCATGCATTACCAATTTATCAAATAAAGGTTAACTTGGTCAAAATTAATTTCCCAGATTAATATGCATCTATAAGTCTAAGGAACCTTCTGCCCAAGTATAGGCTAAAATTAGTTTATGTTTTTTCTTTTTTGAATGCAGGTTGCTCCTGCAGAGTTAGAGGCTATTTTGCTGTCTCATTCCTCAGTTGAAGATGCAGCTGTTGTACCGTAAGTAACCGCGCCCGCGACAATCATTTTTGTTATTCTTTTTAATGTTACCTCTTCATTGTGGATTTAAGATCGAGTTTAATTTAAATATACCGCCGGTGTAAAAGGATCAACATATCCACAGATCACAATCAATCATATGTGTGATTTTAGAAATAGTTACTCTTAAAGTCAAACATTTTTAATCGACGGTCTATACGAATTAAATCTTATAAGATTTCAACTATTTACAATGTTATGTTGTTTTGTGCAATGATTATTACAGATTACCAGATGAAGAGGCAGGGGAAATCCCAGCTGCAAGTGTTGTTTTGAGCAAAGGTGCAAAAGAGAGTGAAGAAGATATTATGAACTACGTTGCTTCCAATGCTGCTCATTACAAGAAAGTGAGAGTGGTTCACTTTGTGGAAGCTATACCAAAATCACCTTCTGGTAAAATAATGAGAAGGCTTGTCAAAGAGAAGATGGTGGAAAAGATTAAGGAAAATAATTCCCTAACAAAATCTAACAATTTTTAA
Protein sequence

>MS.gene04193.t1

MGAYIENFVEENEHIFRSQYSPIPVPENVTLPEFVLQNAELYGDKVAFVDAESGKGVTYSEVVRDIHRFSKALRSLGLRKGNVVIVVLPNIVEYAIIALGIMDAGCVFSGANPASHTSEIKKQVESADAKLIVTNSSSHEKVKSLGLPVIVLGDELVEDAMNWNKLLEAGDRAGNDSMKEPIHQNDLCAMPFSSGTTGMSKGVMLTHRNLVANLCSTLFGVTQEMIGKVTTLGLIPFFHIYGITGICCATIRNKGKVVVMGKYDLKTFLNALITHEVTFAPIVPPIILGLVKNPIVDQFDLSKLKLQAIMTAAAPLAPELLTSFEHKFPGVLVQEAYGLTEHSCITLTYAQKLSGSTHKNSVGFILPNLEVKFIDPETGRSLPRNTPGELCVRSQCVMQGYYKQVDETAQTIDKNGWLHTGDIGFIDDEENVFIVDRIKELIKYKGFQVAPAELEAILLSHSSVEDAAVVPLPDEEAGEIPAASVVLSKGAKESEEDIMNYVASNAAHYKKVRVVHFVEAIPKSPSGKIMRRLVKEKMVEKIKENNSLTKSNNF