AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24088

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24088.t1 MTR_1g026310 95.536 560 18 1 1 553 1 560 0.0 1096
MS.gene24088.t1 MTR_8g039720 80.071 567 91 6 1 553 1 559 0.0 906
MS.gene24088.t1 MTR_1g026470 94.802 404 14 1 1 397 1 404 0.0 779
MS.gene24088.t1 MTR_1g026430 95.511 401 11 1 1 394 1 401 0.0 779
MS.gene24088.t1 MTR_4g057730 45.672 543 286 5 17 553 3 542 1.99e-161 471
MS.gene24088.t1 MTR_3g088870 45.307 554 270 11 14 549 16 554 1.01e-159 467
MS.gene24088.t1 MTR_5g027480 43.571 560 304 6 1 553 1 555 5.69e-158 462
MS.gene24088.t1 MTR_1g090737 46.875 544 271 8 15 546 22 559 1.62e-157 462
MS.gene24088.t1 MTR_3g088880 44.464 560 277 12 7 549 10 552 5.25e-157 460
MS.gene24088.t1 MTR_2g095510 43.693 547 288 6 17 553 3 539 1.59e-152 448
MS.gene24088.t1 MTR_5g027500 44.790 547 278 9 26 553 27 568 2.40e-151 446
MS.gene24088.t1 MTR_4g077900 46.266 482 247 5 80 553 1 478 1.04e-150 441
MS.gene24088.t1 MTR_1g063110 43.808 541 288 8 27 553 13 551 1.85e-144 428
MS.gene24088.t1 MTR_1g063120 43.486 545 288 8 27 553 13 555 1.39e-142 423
MS.gene24088.t1 MTR_1g073180 44.096 542 279 10 14 546 1 527 2.71e-139 414
MS.gene24088.t1 MTR_4g057730 44.068 472 255 5 17 482 3 471 1.01e-129 387
MS.gene24088.t1 MTR_1g073180 42.830 530 276 11 8 528 20 531 2.17e-125 378
MS.gene24088.t1 MTR_2g105570 40.075 534 302 8 26 545 12 541 6.02e-120 365
MS.gene24088.t1 MTR_4g057730 43.111 450 247 5 17 460 3 449 2.20e-118 357
MS.gene24088.t1 MTR_4g128337 38.403 526 316 4 26 545 17 540 1.51e-117 358
MS.gene24088.t1 MTR_3g460770 35.075 536 324 7 20 543 8 531 1.17e-115 354
MS.gene24088.t1 MTR_5g007640 38.649 533 306 7 26 545 55 579 1.77e-115 354
MS.gene24088.t1 MTR_4g005750 40.152 528 304 8 26 545 11 534 3.57e-113 347
MS.gene24088.t1 MTR_2g095510 40.749 454 249 6 17 460 3 446 8.16e-108 330
MS.gene24088.t1 MTR_1g026350 94.118 153 9 0 1 153 1 153 1.04e-100 301
MS.gene24088.t1 MTR_1569s0010 28.215 521 316 12 68 545 22 527 1.32e-54 193
MS.gene24088.t1 MTR_3g035130 28.684 509 336 13 44 543 15 505 2.95e-51 184
MS.gene24088.t1 MTR_8g074040 28.431 510 314 16 67 542 81 573 7.27e-47 173
MS.gene24088.t1 MTR_3g064420 26.908 524 326 13 65 545 37 546 2.82e-45 168
MS.gene24088.t1 MTR_5g008800 27.621 496 326 12 68 539 40 526 1.23e-43 163
MS.gene24088.t1 MTR_5g076240 26.755 527 319 13 68 549 40 544 3.75e-42 159
MS.gene24088.t1 MTR_3g035130 28.947 456 297 13 44 490 15 452 1.71e-41 155
MS.gene24088.t1 MTR_8g074040 28.633 461 278 16 116 542 12 455 2.01e-40 153
MS.gene24088.t1 MTR_4g013210 29.563 389 238 11 192 552 65 445 2.49e-39 149
MS.gene24088.t1 MTR_5g008820 25.605 496 334 13 68 539 40 524 9.03e-39 149
MS.gene24088.t1 MTR_0803s0020 25.979 485 337 11 67 539 260 734 1.21e-38 152
MS.gene24088.t1 MTR_8g074120 27.101 476 302 15 98 542 23 484 1.97e-37 145
MS.gene24088.t1 MTR_2g044180 24.723 542 359 17 49 553 29 558 1.40e-36 143
MS.gene24088.t1 MTR_5g076220 25.528 521 334 11 68 549 40 545 1.96e-36 143
MS.gene24088.t1 MTR_4g011790 26.440 382 246 9 197 552 226 598 2.39e-36 143
MS.gene24088.t1 MTR_5g076200 25.434 519 337 11 68 549 40 545 2.61e-36 142
MS.gene24088.t1 MTR_4g121600 24.859 531 346 15 67 553 45 566 4.66e-35 139
MS.gene24088.t1 MTR_0161s0050 26.232 568 355 16 24 549 2 547 1.36e-34 138
MS.gene24088.t1 MTR_4g011790 27.110 391 241 12 197 552 226 607 1.81e-34 138
MS.gene24088.t1 MTR_4g011790 25.946 370 239 9 197 540 226 586 9.21e-34 136
MS.gene24088.t1 MTR_7g084850 23.092 524 363 11 68 553 203 724 1.58e-33 136
MS.gene24088.t1 MTR_2g044180 27.660 376 246 12 195 553 101 467 2.42e-33 133
MS.gene24088.t1 MTR_4g013210 24.660 515 327 17 52 521 68 566 5.39e-33 133
MS.gene24088.t1 MTR_4g013210 29.521 376 214 14 192 552 65 404 2.14e-31 126
MS.gene24088.t1 MTR_5g023730 24.606 508 335 16 67 542 42 533 3.36e-31 127
MS.gene24088.t1 MTR_4g013210 25.612 531 323 21 52 552 68 556 4.58e-31 127
MS.gene24088.t1 MTR_8g083170 25.919 571 301 18 69 528 110 669 4.03e-29 122
MS.gene24088.t1 MTR_3g084470 25.989 531 275 18 69 491 106 626 9.09e-28 118
MS.gene24088.t1 MTR_2g030240 25.282 443 299 11 69 485 29 465 3.97e-27 115
MS.gene24088.t1 MTR_2g030240 27.243 301 196 7 202 485 77 371 5.31e-24 105
MS.gene24088.t1 MTR_3g088890 60.938 64 25 0 455 518 10 73 1.74e-22 92.4
MS.gene24088.t1 MTR_1g104810 25.109 458 256 16 62 466 141 564 1.68e-21 99.0
MS.gene24088.t1 MTR_1g104810 25.328 458 255 15 62 466 74 497 2.60e-21 98.2
MS.gene24088.t1 MTR_4g057755 57.534 73 31 0 409 481 46 118 2.81e-21 90.1
MS.gene24088.t1 MTR_1054s0020 23.529 493 347 16 65 547 294 766 8.10e-21 97.1
MS.gene24088.t1 MTR_3g087980 27.697 343 174 15 198 475 248 581 3.79e-20 94.7
MS.gene24088.t1 MTR_1g086600 25.749 334 192 11 198 480 243 571 1.21e-19 93.2
MS.gene24088.t1 MTR_1g030290 28.235 340 176 13 198 475 250 583 2.24e-19 92.4
MS.gene24088.t1 MTR_4g066130 25.734 443 268 17 158 552 619 1048 4.97e-19 92.0
MS.gene24088.t1 MTR_2g098490 24.943 441 266 17 158 547 614 1040 1.02e-18 90.9
MS.gene24088.t1 MTR_8g099420 24.850 334 187 12 196 475 222 545 2.24e-18 89.4
MS.gene24088.t1 MTR_4g013210 24.429 438 271 18 52 470 68 464 9.46e-17 83.6
MS.gene24088.t1 MTR_3g084450 33.503 197 114 5 322 505 246 438 1.38e-16 83.2
MS.gene24088.t1 MTR_3g084450 33.503 197 114 5 322 505 454 646 2.93e-16 82.8
MS.gene24088.t1 MTR_7g109830 34.756 164 99 6 321 480 388 547 5.94e-15 78.6
MS.gene24088.t1 MTR_4g068670 22.626 495 295 19 62 485 72 549 3.64e-14 75.9
MS.gene24088.t1 MTR_5g009350 22.659 331 200 10 195 475 220 544 8.29e-14 74.7
MS.gene24088.t1 MTR_5g009350 22.659 331 200 10 195 475 220 544 9.24e-14 74.3
MS.gene24088.t1 MTR_5g009350 23.030 330 198 10 196 475 221 544 1.06e-13 74.3
MS.gene24088.t1 MTR_5g009350 21.905 420 250 14 196 546 221 631 1.19e-13 74.3
MS.gene24088.t1 MTR_4g013210 22.581 403 260 15 52 419 68 453 1.78e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24088.t1 AT1G20510 74.630 540 134 2 14 551 4 542 0.0 827
MS.gene24088.t1 AT1G20500 66.302 549 170 5 13 553 9 550 0.0 737
MS.gene24088.t1 AT1G20510 73.673 471 121 2 14 482 4 473 0.0 710
MS.gene24088.t1 AT1G20480 61.429 560 202 4 4 553 10 565 0.0 706
MS.gene24088.t1 AT5G38120 61.580 557 193 7 9 553 3 550 0.0 691
MS.gene24088.t1 AT1G20510 73.051 449 118 2 14 460 4 451 0.0 673
MS.gene24088.t1 AT5G38120 60.269 521 186 7 9 517 3 514 0.0 633
MS.gene24088.t1 AT5G38120 60.494 486 171 7 9 482 3 479 0.0 589
MS.gene24088.t1 AT5G38120 60.494 486 171 7 9 482 3 479 0.0 589
MS.gene24088.t1 AT1G20490 60.047 428 154 5 13 430 9 429 7.89e-179 511
MS.gene24088.t1 AT1G20490 60.047 428 154 5 13 430 9 429 7.89e-179 511
MS.gene24088.t1 AT5G63380 43.162 563 286 8 8 549 5 554 1.10e-164 480
MS.gene24088.t1 AT4G19010 41.591 553 299 7 15 553 20 562 2.76e-155 456
MS.gene24088.t1 AT4G05160 43.876 547 292 7 17 553 3 544 6.96e-155 454
MS.gene24088.t1 AT3G21240 39.474 532 313 6 21 545 17 546 1.55e-120 367
MS.gene24088.t1 AT1G65060 40.643 529 299 6 26 545 34 556 6.62e-120 365
MS.gene24088.t1 AT1G51680 39.377 546 317 6 12 545 10 553 3.88e-112 345
MS.gene24088.t1 AT3G21230 38.632 541 311 9 22 545 24 560 2.04e-110 341
MS.gene24088.t1 AT1G62940 35.619 525 329 4 26 545 12 532 3.47e-102 319
MS.gene24088.t1 AT1G51680 37.452 518 310 6 12 517 10 525 2.06e-97 306
MS.gene24088.t1 AT3G21230 37.115 520 302 10 22 524 24 535 3.13e-97 306
MS.gene24088.t1 AT3G21240 37.500 472 286 6 21 485 17 486 6.95e-96 301
MS.gene24088.t1 AT3G21240 37.500 472 286 6 21 485 17 486 6.95e-96 301
MS.gene24088.t1 AT3G21230 37.107 477 279 9 22 481 24 496 3.20e-88 281
MS.gene24088.t1 AT1G51680 37.137 482 289 6 12 481 10 489 1.68e-87 279
MS.gene24088.t1 AT1G65060 37.220 446 265 6 26 462 34 473 3.95e-81 263
MS.gene24088.t1 AT3G21230 32.678 407 253 9 22 411 24 426 1.43e-51 183
MS.gene24088.t1 AT3G48990 28.008 482 335 10 67 543 30 504 4.92e-47 172
MS.gene24088.t1 AT1G65890 28.060 531 317 18 67 549 39 552 1.32e-46 172
MS.gene24088.t1 AT1G21530 27.788 529 319 16 62 549 37 543 2.45e-46 171
MS.gene24088.t1 AT1G77240 26.705 528 337 15 58 551 32 543 4.07e-46 170
MS.gene24088.t1 AT1G20560 27.188 537 348 14 34 542 14 535 4.40e-46 170
MS.gene24088.t1 AT1G65880 28.652 534 311 19 67 549 39 553 6.11e-46 170
MS.gene24088.t1 AT1G21530 27.514 527 325 15 62 549 37 545 6.39e-46 169
MS.gene24088.t1 AT1G21540 27.938 519 322 14 69 551 46 548 1.79e-45 168
MS.gene24088.t1 AT1G66120 28.000 525 322 15 67 551 39 547 8.97e-45 167
MS.gene24088.t1 AT1G76290 28.101 516 318 18 67 549 39 534 7.10e-44 164
MS.gene24088.t1 AT5G16370 26.628 522 326 14 74 553 43 549 1.76e-41 157
MS.gene24088.t1 AT2G17650 27.013 559 346 19 37 549 55 597 3.16e-40 154
MS.gene24088.t1 AT5G16340 26.796 515 321 14 74 547 43 542 1.54e-39 152
MS.gene24088.t1 AT1G20560 27.692 455 292 12 116 542 12 457 2.80e-39 150
MS.gene24088.t1 AT1G30520 27.155 464 292 11 64 496 34 482 1.77e-38 149
MS.gene24088.t1 AT1G30520 27.155 464 292 11 64 496 34 482 1.77e-38 149
MS.gene24088.t1 AT1G75960 26.488 521 324 14 68 549 41 541 4.86e-37 144
MS.gene24088.t1 AT1G68270 24.952 521 308 16 67 545 39 518 8.05e-34 135
MS.gene24088.t1 AT3G16170 24.672 381 245 9 197 547 234 602 2.89e-32 131
MS.gene24088.t1 AT3G16910 23.269 520 346 16 67 545 47 554 7.93e-32 130
MS.gene24088.t1 AT5G36880 22.761 536 362 13 60 553 144 669 1.09e-31 130
MS.gene24088.t1 AT5G36880 22.761 536 362 13 60 553 144 669 1.09e-31 130
MS.gene24088.t1 AT5G36880 22.761 536 362 13 60 553 144 669 1.09e-31 130
MS.gene24088.t1 AT5G36880 22.761 536 362 13 60 553 144 669 1.09e-31 130
MS.gene24088.t1 AT5G36880 22.948 536 361 14 60 553 194 719 1.49e-31 130
MS.gene24088.t1 AT1G30520 25.843 445 281 12 64 477 34 460 5.88e-31 126
MS.gene24088.t1 AT1G30520 25.843 445 281 12 64 477 34 460 5.88e-31 126
MS.gene24088.t1 AT5G36880 21.957 460 312 11 60 481 144 594 1.37e-24 108
MS.gene24088.t1 AT5G27600 27.760 317 172 9 197 466 261 567 2.08e-22 102
MS.gene24088.t1 AT3G05970 28.707 317 169 9 197 466 261 567 5.42e-20 94.4
MS.gene24088.t1 AT2G47240 26.857 350 199 12 180 480 147 488 1.19e-19 93.2
MS.gene24088.t1 AT2G47240 26.857 350 199 12 180 480 206 547 1.23e-19 93.2
MS.gene24088.t1 AT2G47240 26.857 350 199 12 180 480 206 547 1.23e-19 93.2
MS.gene24088.t1 AT2G47240 26.857 350 199 12 180 480 206 547 1.23e-19 93.2
MS.gene24088.t1 AT1G64400 22.515 493 303 19 55 478 65 547 1.52e-19 92.8
MS.gene24088.t1 AT1G77590 27.874 348 167 14 198 475 100 433 2.74e-19 91.7
MS.gene24088.t1 AT1G77590 23.881 536 297 21 23 475 72 579 3.92e-19 91.7
MS.gene24088.t1 AT1G49430 25.000 344 199 13 193 485 219 554 1.46e-16 83.6
MS.gene24088.t1 AT4G11030 23.548 310 174 10 198 454 224 523 6.31e-15 78.2
MS.gene24088.t1 AT3G23790 32.632 190 103 6 322 490 455 640 3.41e-13 72.8
MS.gene24088.t1 AT3G23790 24.390 246 153 9 68 282 111 354 9.33e-12 68.2
MS.gene24088.t1 AT4G14070 29.200 250 146 8 322 546 471 714 5.57e-12 68.9

Find 158 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGCCCAGCCTCCTTATT+TGG 0.136723 1.2:-63694 None:intergenic
TCGCTTCATACGGTGGTTTC+TGG 0.138183 1.2:+61886 MS.gene24088:CDS
CTGCTGCAAAACCCACTTCT+TGG 0.251921 1.2:-60983 None:intergenic
ACCACTTGTGCCGCCGATAC+TGG 0.271545 1.2:+61813 MS.gene24088:CDS
GTAAGTATCTTTCAAGGTTA+TGG 0.275288 1.2:+61964 MS.gene24088:CDS
TGGAGCTTCTACTGAGTCTT+TGG 0.282200 1.2:+62008 MS.gene24088:CDS
TGTTGTTGATGATGAGTTGC+TGG 0.335506 1.2:-61440 None:intergenic
GTTCCGATGTTTCATATATA+CGG 0.342157 1.2:+61676 MS.gene24088:CDS
TGCTATGGGGCTTCTTGCTA+TGG 0.342915 1.2:+61711 MS.gene24088:CDS
TTAACTGAATCCTCTGGAAT+TGG 0.351281 1.2:+61988 MS.gene24088:CDS
AGCTCACCAGTCCGATTAAC+TGG 0.354985 1.2:-62117 None:intergenic
CTCTACTCATCAGGAACAAC+TGG 0.359656 1.2:+61544 MS.gene24088:CDS
CAAGTTGTAATGGCTAGATT+TGG 0.372468 1.2:+61616 MS.gene24088:CDS
GGAGGATGACGTCACCTTTA+CGG 0.372564 1.2:-61204 None:intergenic
TTATATCAGGTATCCAAATA+AGG 0.373402 1.2:+63681 MS.gene24088:intron
CATTAAGTACAAGGGATATC+AGG 0.379179 1.2:+63263 MS.gene24088:CDS
GGTGAATTGGTGGCCGGTGG+AGG 0.402061 1.2:-61122 None:intergenic
ATGACGGAGAGGCAGACAAC+AGG 0.402334 1.2:-61253 None:intergenic
AAGGCTGGAAGTAGCTTGTC+AGG 0.410549 1.2:+63739 MS.gene24088:CDS
TTAATCGGACTGGTGAGCTT+TGG 0.414553 1.2:+62121 MS.gene24088:CDS
TGGAGTTGGGAGAGAGGAGG+AGG 0.419067 1.2:-61222 None:intergenic
GAATTTAGAGAGAACAACTA+TGG 0.419281 1.2:-61740 None:intergenic
AAGAAACGAACCTTTCATAA+TGG 0.419398 1.2:-62157 None:intergenic
AGTAGATGGAGTTGGGAGAG+AGG 0.420482 1.2:-61228 None:intergenic
TGGTTTGGTGGAAGTGGAAG+GGG 0.421746 1.2:-61034 None:intergenic
ACTGGTGAGCTTTGGCTTCG+TGG 0.421933 1.2:+62129 MS.gene24088:CDS
AGTGAAAGCAAGAACAGGTT+TGG 0.423538 1.2:-61350 None:intergenic
ATCCATCTGGTAAAATTCTT+CGG 0.423771 1.2:+63958 MS.gene24088:CDS
AAAGAGTGGTTTGGTGGAAG+TGG 0.424298 1.2:-61040 None:intergenic
CCTTGAAAGATACTTACATT+AGG 0.428705 1.2:-61958 None:intergenic
TCCAAATAAGGAGGCTGGGC+AGG 0.431867 1.2:+63693 MS.gene24088:CDS
CCTAATGTAAGTATCTTTCA+AGG 0.432845 1.2:+61958 MS.gene24088:CDS
CTCAGCCGTTACCAGTTAAT+CGG 0.436385 1.2:+62106 MS.gene24088:CDS
GGTCTTGCAGTGTTTGCTAT+GGG 0.437424 1.2:+61697 MS.gene24088:CDS
CGGTCTTGCAGTGTTTGCTA+TGG 0.438320 1.2:+61696 MS.gene24088:CDS
GCTATGGGGCTTCTTGCTAT+GGG 0.440856 1.2:+61712 MS.gene24088:CDS
CTACAGCACGCCAGAGTTGT+TGG 0.441513 1.2:-61147 None:intergenic
GCAGACAACAGGGAAGTAGA+TGG 0.455653 1.2:-61242 None:intergenic
CTTGTCAGGAAGCCAAGTTA+TGG 0.458124 1.2:+63753 MS.gene24088:CDS
TTGGAAGAGAGTCGTAAGTA+TGG 0.463115 1.2:+62027 MS.gene24088:CDS
AGGCGTCAATGAAGGCGATA+TGG 0.463838 1.2:-61102 None:intergenic
GGGGCTTGTGTCTGCTTCCA+CGG 0.471317 1.2:+62056 MS.gene24088:CDS
TATGGTTTAACTGAATCCTC+TGG 0.471540 1.2:+61982 MS.gene24088:CDS
GTGGTTTGGTGGAAGTGGAA+GGG 0.473586 1.2:-61035 None:intergenic
AGAGTTGTTGGTAGGTGAAT+TGG 0.474858 1.2:-61135 None:intergenic
AGGTATCCAAATAAGGAGGC+TGG 0.481674 1.2:+63688 MS.gene24088:intron
TTCACTTCCAATCATTCTCA+TGG 0.482486 1.2:+61411 MS.gene24088:CDS
TAACCAACCCTCTGGAGTAA+TGG 0.484888 1.2:-63172 None:intergenic
CGATTTCGCGAGATGTATTG+AGG 0.485277 1.2:-61309 None:intergenic
GCGAGATGTATTGAGGGGAT+TGG 0.486320 1.2:-61302 None:intergenic
AACAAAGCCTCTAGTTCTGC+TGG 0.486762 1.2:-63474 None:intergenic
CACTTCCTCACTCTCCAACA+TGG 0.488973 1.2:+61177 MS.gene24088:CDS
GCCGGTGGAGGCGTCAATGA+AGG 0.490250 1.2:-61110 None:intergenic
AGTGGTTTGGTGGAAGTGGA+AGG 0.490859 1.2:-61036 None:intergenic
GCATATGTGGTAAGGAAGGC+TGG 0.491572 1.2:+63724 MS.gene24088:CDS
ATTAAGAAGATGGAGGTAAA+CGG 0.492454 1.2:+60929 None:intergenic
TGTATTGAGGGGATTGGTGG+TGG 0.493354 1.2:-61296 None:intergenic
CGATGGATTCTTATTTGTCG+TGG 0.494381 1.2:+63224 MS.gene24088:CDS
GTGGTGTCCATGAGAATGAT+TGG 0.496058 1.2:-61418 None:intergenic
CTGCAACAAAATCCATAACT+TGG 0.502133 1.2:-63765 None:intergenic
ACAGGGAAGTAGATGGAGTT+GGG 0.504299 1.2:-61235 None:intergenic
TGATTTGAGTTCGCTTCATA+CGG 0.505643 1.2:+61876 MS.gene24088:CDS
TTGGTAGGTGAATTGGTGGC+CGG 0.506146 1.2:-61128 None:intergenic
GCCACACTACTCTACTCATC+AGG 0.506955 1.2:+61535 MS.gene24088:CDS
AGTCGTAAGTATGGTACGGC+GGG 0.509532 1.2:+62036 MS.gene24088:CDS
AACAGGGAAGTAGATGGAGT+TGG 0.510251 1.2:-61236 None:intergenic
CGGCGGCACAAGTGGTAAGA+AGG 0.513016 1.2:-61806 None:intergenic
CATCGCTATGAGATTCTTGT+GGG 0.513470 1.2:-61590 None:intergenic
GCCTTCATTGACGCCTCCAC+CGG 0.515250 1.2:+61109 MS.gene24088:CDS
TGGAAGTGAAGGTGATGCTT+GGG 0.520153 1.2:-61398 None:intergenic
GGAGTAGTGAAAGCAAGAAC+AGG 0.520555 1.2:-61355 None:intergenic
GAAGATACTACTCCTTTGCT+TGG 0.520592 1.2:-61568 None:intergenic
ACTTCCTCACTCTCCAACAT+GGG 0.520727 1.2:+61178 MS.gene24088:CDS
TGCCTCTCCGTCATGTCCCT+CGG 0.521898 1.2:+61262 MS.gene24088:CDS
TTCCTTACCACATATGCCAT+TGG 0.522529 1.2:-63718 None:intergenic
AGCGATGGTTCAAGTTGTAA+TGG 0.525827 1.2:+61606 MS.gene24088:CDS
TCCGTTACTTCTTTACTCAA+AGG 0.528090 1.2:-61916 None:intergenic
GTTTGCTATATTGATAGCGA+TGG 0.538596 1.2:+63207 MS.gene24088:CDS
TTGGAAGTGAAGGTGATGCT+TGG 0.539417 1.2:-61399 None:intergenic
ATACAAAAGAATTCGAAAAG+TGG 0.543360 1.2:+63911 MS.gene24088:CDS
TTCCAATGGCATATGTGGTA+AGG 0.544112 1.2:+63716 MS.gene24088:CDS
CTTCATACGGTGGTTTCTGG+TGG 0.547015 1.2:+61889 MS.gene24088:CDS
ATCTGGTAAAATTCTTCGGA+AGG 0.547613 1.2:+63962 MS.gene24088:CDS
GGAGGCTGGGCAGGTTCCAA+TGG 0.547827 1.2:+63702 MS.gene24088:CDS
ACAGCAGCGTCTACAATAGC+AGG 0.550475 1.2:-63507 None:intergenic
GTAGGTGAATTGGTGGCCGG+TGG 0.552038 1.2:-61125 None:intergenic
ACCTTTACGGATCCCCATGT+TGG 0.552224 1.2:-61191 None:intergenic
GAAAGAGCTCATTAAGTACA+AGG 0.552506 1.2:+63254 MS.gene24088:CDS
GCATCTAAAGAGTGGTTTGG+TGG 0.553390 1.2:-61046 None:intergenic
CGTGGTCCCACCATTATGAA+AGG 0.554145 1.2:+62147 MS.gene24088:CDS
TCCAACATGGGGATCCGTAA+AGG 0.554642 1.2:+61190 MS.gene24088:CDS
GCAGGTTCCAATGGCATATG+TGG 0.556198 1.2:+63711 MS.gene24088:CDS
ACCAGTATCGGCGGCACAAG+TGG 0.558595 1.2:-61814 None:intergenic
AAACGAACCTTTCATAATGG+TGG 0.559397 1.2:-62154 None:intergenic
TTCAGCATAGCAACCAGTAT+CGG 0.561145 1.2:-61826 None:intergenic
CCAGTCCGATTAACTGGTAA+CGG 0.565679 1.2:-62111 None:intergenic
GGTATCCAAATAAGGAGGCT+GGG 0.569081 1.2:+63689 MS.gene24088:intron
CCATCGCTATGAGATTCTTG+TGG 0.569419 1.2:-61591 None:intergenic
GTGGCATCTAAAGAGTGGTT+TGG 0.570177 1.2:-61049 None:intergenic
CCGTTACCAGTTAATCGGAC+TGG 0.573347 1.2:+62111 MS.gene24088:CDS
TTGAGTAAAGAAGTAACGGA+AGG 0.574145 1.2:+61919 MS.gene24088:CDS
AGATGTATTGAGGGGATTGG+TGG 0.576476 1.2:-61299 None:intergenic
ATCTCCTCCCGCGCTCATCA+CGG 0.577531 1.2:+61079 MS.gene24088:CDS
TCCTTTGAGTAAAGAAGTAA+CGG 0.584099 1.2:+61915 MS.gene24088:CDS
GATTTCGCGAGATGTATTGA+GGG 0.584385 1.2:-61308 None:intergenic
AATTCACCTACCAACAACTC+TGG 0.585675 1.2:+61137 MS.gene24088:CDS
AACGAACCTTTCATAATGGT+GGG 0.587290 1.2:-62153 None:intergenic
GATATGGCCGTGATGAGCGC+GGG 0.587698 1.2:-61086 None:intergenic
AGATGGAGTTGGGAGAGAGG+AGG 0.592217 1.2:-61225 None:intergenic
TGAGTAAAGAAGTAACGGAA+GGG 0.592240 1.2:+61920 MS.gene24088:CDS
GTTGTTGGTAGGTGAATTGG+TGG 0.596032 1.2:-61132 None:intergenic
AATGGCATATGTGGTAAGGA+AGG 0.598196 1.2:+63720 MS.gene24088:CDS
GTCTTGCAGTGTTTGCTATG+GGG 0.598502 1.2:+61698 MS.gene24088:CDS
CGATATGGCCGTGATGAGCG+CGG 0.598855 1.2:-61087 None:intergenic
TGACGGAGAGGCAGACAACA+GGG 0.598859 1.2:-61252 None:intergenic
GTTGGAGAGTGAGGAAGTGA+CGG 0.598886 1.2:-61173 None:intergenic
CGACCATTACTCCAGAGGGT+TGG 0.603546 1.2:+63169 MS.gene24088:CDS
GATAGCGCCGAGGGACATGA+CGG 0.604148 1.2:-61269 None:intergenic
AGAGTGATCGATCCAAGAAG+TGG 0.604701 1.2:+60971 MS.gene24088:CDS
AGTGACTGAGCGCTTCAACC+AGG 0.605836 1.2:+61504 MS.gene24088:CDS
TGTTGATGATGAGTTGCTGG+TGG 0.609037 1.2:-61437 None:intergenic
ATGGCCGTGATGAGCGCGGG+AGG 0.610511 1.2:-61083 None:intergenic
CCACAAGAATCTCATAGCGA+TGG 0.611191 1.2:+61591 MS.gene24088:CDS
GGTCCCTCCAGCAGAACTAG+AGG 0.614140 1.2:+63467 MS.gene24088:intron
TTTGAGTTCGCTTCATACGG+TGG 0.616174 1.2:+61879 MS.gene24088:CDS
GATCCCCATGTTGGAGAGTG+AGG 0.617341 1.2:-61182 None:intergenic
GGTGGTGATGATAGCGCCGA+GGG 0.619275 1.2:-61278 None:intergenic
AGAGAGTCGTAAGTATGGTA+CGG 0.623595 1.2:+62032 MS.gene24088:CDS
AGCGCGGGAGGAGATGAAAG+TGG 0.628660 1.2:-61071 None:intergenic
GGAACAACTGGACCAAGCAA+AGG 0.634924 1.2:+61556 MS.gene24088:CDS
TCAGTCACTCTACTCAACTC+AGG 0.638010 1.2:-61490 None:intergenic
GTAGTGTGGCTGTATCATCC+TGG 0.639019 1.2:-61522 None:intergenic
ATGAGAATGATTGGAAGTGA+AGG 0.639263 1.2:-61409 None:intergenic
AAAGCCTCTAGTTCTGCTGG+AGG 0.643862 1.2:-63471 None:intergenic
AGCATAGCAACCAGTATCGG+CGG 0.644393 1.2:-61823 None:intergenic
GGCAGGTTATTTCAGTAACG+AGG 0.647450 1.2:+63134 MS.gene24088:intron
ACATCGACCATTACTCCAGA+GGG 0.647619 1.2:+63165 MS.gene24088:CDS
AAAGAGCTCATTAAGTACAA+GGG 0.651964 1.2:+63255 MS.gene24088:CDS
GAGTGATCGATCCAAGAAGT+GGG 0.652473 1.2:+60972 MS.gene24088:CDS
AGACCGTATATATGAAACAT+CGG 0.653796 1.2:-61679 None:intergenic
GCTTGTGTCTGCTTCCACGG+AGG 0.655096 1.2:+62059 MS.gene24088:CDS
CTCTGGAGTAATGGTCGATG+TGG 0.656546 1.2:-63163 None:intergenic
AAGCCTCTAGTTCTGCTGGA+GGG 0.656701 1.2:-63470 None:intergenic
ATTTCGCGAGATGTATTGAG+GGG 0.663874 1.2:-61307 None:intergenic
CTTCCTCACTCTCCAACATG+GGG 0.667378 1.2:+61179 MS.gene24088:CDS
GCGGGAGGAGATGAAAGTGG+TGG 0.667975 1.2:-61068 None:intergenic
AGGTTATTTCAGTAACGAGG+AGG 0.670840 1.2:+63137 MS.gene24088:intron
CGCCGAGGGACATGACGGAG+AGG 0.671164 1.2:-61264 None:intergenic
AAGTGGTGGCATCTAAAGAG+TGG 0.673146 1.2:-61054 None:intergenic
TATCAGGTATCCAAATAAGG+AGG 0.676684 1.2:+63684 MS.gene24088:intron
CACATCGACCATTACTCCAG+AGG 0.683319 1.2:+63164 MS.gene24088:CDS
TCCTGATGAGTAGAGTAGTG+TGG 0.691172 1.2:-61536 None:intergenic
GTCGTAAGTATGGTACGGCG+GGG 0.691371 1.2:+62037 MS.gene24088:CDS
AGCACGCCAGAGTTGTTGGT+AGG 0.696936 1.2:-61143 None:intergenic
AGTAGAAGCTCCAATTCCAG+AGG 0.697004 1.2:-61998 None:intergenic
TGGTGGTGATGATAGCGCCG+AGG 0.700534 1.2:-61279 None:intergenic
GTCAACAATCATAGCCTCCG+TGG 0.700669 1.2:-62073 None:intergenic
GAGTCGTAAGTATGGTACGG+CGG 0.704214 1.2:+62035 MS.gene24088:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTAATTAATTAAAAA+AGG - chr1.2:62315-62334 None:intergenic 0.0%
!! ATTAATTTATATTCTTTGGT+AGG - chr1.2:63544-63563 None:intergenic 15.0%
!! GAAAAAAATTAAAGTTGTAT+TGG - chr1.2:62517-62536 None:intergenic 15.0%
!!! AATTTTTTTCAATATATAGG+TGG + chr1.2:63884-63903 MS.gene24088:intron 15.0%
!! AAATGGAATGAAATAATCAT+TGG - chr1.2:62642-62661 None:intergenic 20.0%
!! AGTAAAATGTCAAAATTGTT+AGG - chr1.2:62847-62866 None:intergenic 20.0%
!! ATAAACAATTCAGATTAGAT+TGG + chr1.2:62242-62261 MS.gene24088:intron 20.0%
!! TAATTAAAATATTGAACACG+AGG + chr1.2:62323-62342 MS.gene24088:intron 20.0%
!!! ATCGATATTCTAAAAAATGT+GGG - chr1.2:62752-62771 None:intergenic 20.0%
!!! GTTACTTTTTTTATGTCTAA+TGG - chr1.2:62946-62965 None:intergenic 20.0%
!!! TATCGATATTCTAAAAAATG+TGG - chr1.2:62753-62772 None:intergenic 20.0%
!!! TGTCTTTTTCTAATAAAGTA+AGG + chr1.2:62593-62612 MS.gene24088:intron 20.0%
!!! TTTTGTGTGTATATTATATC+AGG + chr1.2:63668-63687 MS.gene24088:intron 20.0%
! AATTATAGCATATAAACTCG+TGG - chr1.2:62291-62310 None:intergenic 25.0%
! ATACAAAAGAATTCGAAAAG+TGG + chr1.2:63911-63930 MS.gene24088:CDS 25.0%
! ATATAAGCTCACATAATTGT+TGG + chr1.2:63067-63086 MS.gene24088:intron 25.0%
! CACTAAAATAATAGATGCAA+AGG + chr1.2:63005-63024 MS.gene24088:intron 25.0%
! GTAAACAGTAATTTATCTCT+GGG - chr1.2:63359-63378 None:intergenic 25.0%
! TCTTTATTACTATTGTGTGT+CGG - chr1.2:63381-63400 None:intergenic 25.0%
! TTATATCAGGTATCCAAATA+AGG + chr1.2:63681-63700 MS.gene24088:intron 25.0%
! TTATTACAAACTCACACAAA+TGG + chr1.2:62791-62810 MS.gene24088:intron 25.0%
!! ACTTTTCGAATTCTTTTGTA+TGG - chr1.2:63912-63931 None:intergenic 25.0%
!! TGTTTTATGGTTATTGTTAC+AGG + chr1.2:63446-63465 MS.gene24088:intron 25.0%
!! TTTTAGTGTTATAAACACCT+TGG - chr1.2:62993-63012 None:intergenic 25.0%
!!! AATTTTACCAGATGGATTTT+TGG - chr1.2:63955-63974 None:intergenic 25.0%
!!! ACTCTTTTTTATTAGATGCT+TGG + chr1.2:63826-63845 MS.gene24088:intron 25.0%
!!! ATTTTACCAGATGGATTTTT+GGG - chr1.2:63954-63973 None:intergenic 25.0%
!!! CATAATTGTTGGTTCTATTA+TGG + chr1.2:63078-63097 MS.gene24088:intron 25.0%
!!! CTCTTTTTTATTAGATGCTT+GGG + chr1.2:63827-63846 MS.gene24088:intron 25.0%
AAACTCACACAAATGGATAT+TGG + chr1.2:62798-62817 MS.gene24088:intron 30.0%
AAAGAGCTCATTAAGTACAA+GGG + chr1.2:63255-63274 MS.gene24088:CDS 30.0%
AACTCACACAAATGGATATT+GGG + chr1.2:62799-62818 MS.gene24088:intron 30.0%
AAGAAACGAACCTTTCATAA+TGG - chr1.2:62160-62179 None:intergenic 30.0%
AGACCGTATATATGAAACAT+CGG - chr1.2:61682-61701 None:intergenic 30.0%
ATCCATCTGGTAAAATTCTT+CGG + chr1.2:63958-63977 MS.gene24088:CDS 30.0%
ATTAAAATGCAAGACATGGT+TGG + chr1.2:63107-63126 MS.gene24088:intron 30.0%
CCTAATGTAAGTATCTTTCA+AGG + chr1.2:61958-61977 MS.gene24088:CDS 30.0%
CCTTGAAAGATACTTACATT+AGG - chr1.2:61961-61980 None:intergenic 30.0%
CGTAAATATTGTCAAACTCA+TGG + chr1.2:63408-63427 MS.gene24088:intron 30.0%
GAATTTAGAGAGAACAACTA+TGG - chr1.2:61743-61762 None:intergenic 30.0%
GCTAATTAAAATGCAAGACA+TGG + chr1.2:63103-63122 MS.gene24088:intron 30.0%
GGTAAACAGTAATTTATCTC+TGG - chr1.2:63360-63379 None:intergenic 30.0%
GTAAGTATCTTTCAAGGTTA+TGG + chr1.2:61964-61983 MS.gene24088:CDS 30.0%
GTTCCGATGTTTCATATATA+CGG + chr1.2:61676-61695 MS.gene24088:CDS 30.0%
TATACACACAAAACATTCAG+CGG - chr1.2:63661-63680 None:intergenic 30.0%
TATATTCTTTGGTAGGCTTA+CGG - chr1.2:63537-63556 None:intergenic 30.0%
TCCTTTGAGTAAAGAAGTAA+CGG + chr1.2:61915-61934 MS.gene24088:CDS 30.0%
TGAATTGCTAAACAAGTTTC+CGG - chr1.2:62354-62373 None:intergenic 30.0%
TGATTGATAAGAAGGAATAC+TGG - chr1.2:63635-63654 None:intergenic 30.0%
TTGACACACAGATTAAGAAA+TGG - chr1.2:62659-62678 None:intergenic 30.0%
!! AAGTGACACTCATTTTGAAA+CGG - chr1.2:62451-62470 None:intergenic 30.0%
!!! GTTCTCTTGCTTTTGTTTTA+TGG + chr1.2:63433-63452 MS.gene24088:intron 30.0%
AAACGAACCTTTCATAATGG+TGG - chr1.2:62157-62176 None:intergenic 35.0%
AAAGTTTGAGAGGGTAAAGT+TGG - chr1.2:62542-62561 None:intergenic 35.0%
AACGAACCTTTCATAATGGT+GGG - chr1.2:62156-62175 None:intergenic 35.0%
AATGAGAACATGTGGTCTAT+TGG + chr1.2:62410-62429 MS.gene24088:intron 35.0%
ACTCACACAAATGGATATTG+GGG + chr1.2:62800-62819 MS.gene24088:intron 35.0%
ATCTGGTAAAATTCTTCGGA+AGG + chr1.2:63962-63981 MS.gene24088:CDS 35.0%
ATGAGAATGATTGGAAGTGA+AGG - chr1.2:61412-61431 None:intergenic 35.0%
CAAGTTGTAATGGCTAGATT+TGG + chr1.2:61616-61635 MS.gene24088:CDS 35.0%
CATTAAGTACAAGGGATATC+AGG + chr1.2:63263-63282 MS.gene24088:CDS 35.0%
CTGCAACAAAATCCATAACT+TGG - chr1.2:63768-63787 None:intergenic 35.0%
GAAAGAGCTCATTAAGTACA+AGG + chr1.2:63254-63273 MS.gene24088:CDS 35.0%
GAATTGCTAAACAAGTTTCC+GGG - chr1.2:62353-62372 None:intergenic 35.0%
TAATACCAGCAACTAACCAA+AGG - chr1.2:63046-63065 None:intergenic 35.0%
TAGATGCAAAGGAAAAAGCT+TGG + chr1.2:63016-63035 MS.gene24088:intron 35.0%
TATCAGGTATCCAAATAAGG+AGG + chr1.2:63684-63703 MS.gene24088:intron 35.0%
TATGGTTTAACTGAATCCTC+TGG + chr1.2:61982-62001 MS.gene24088:CDS 35.0%
TCCGTTACTTCTTTACTCAA+AGG - chr1.2:61919-61938 None:intergenic 35.0%
TCTATACCCAAAAATCCATC+TGG + chr1.2:63945-63964 MS.gene24088:CDS 35.0%
TGTGTGAGAAAAGTTTGAGA+GGG - chr1.2:62551-62570 None:intergenic 35.0%
TTCACTTCCAATCATTCTCA+TGG + chr1.2:61411-61430 MS.gene24088:CDS 35.0%
TTGATGAGAATGAGAACATG+TGG + chr1.2:62402-62421 MS.gene24088:intron 35.0%
TTGTGTGAGAAAAGTTTGAG+AGG - chr1.2:62552-62571 None:intergenic 35.0%
! TGAGTAAAGAAGTAACGGAA+GGG + chr1.2:61920-61939 MS.gene24088:CDS 35.0%
! TGATTTGAGTTCGCTTCATA+CGG + chr1.2:61876-61895 MS.gene24088:CDS 35.0%
! TTCCGAAGAATTTTACCAGA+TGG - chr1.2:63963-63982 None:intergenic 35.0%
! TTGAGTAAAGAAGTAACGGA+AGG + chr1.2:61919-61938 MS.gene24088:CDS 35.0%
!! ATGCTTGGGTGATTTTAGAA+AGG - chr1.2:61387-61406 None:intergenic 35.0%
!! GTGATTTTAGAAAGGAGTTG+TGG - chr1.2:61379-61398 None:intergenic 35.0%
!! GTTTGCTATATTGATAGCGA+TGG + chr1.2:63207-63226 MS.gene24088:CDS 35.0%
!! TTAACTGAATCCTCTGGAAT+TGG + chr1.2:61988-62007 MS.gene24088:CDS 35.0%
!! TTCAAAATGAGTGTCGCTTT+AGG - chr1.2:62684-62703 None:intergenic 35.0%
!!! GACACTCATTTTGAAACAGA+GGG + chr1.2:62689-62708 MS.gene24088:intron 35.0%
AATGGCATATGTGGTAAGGA+AGG + chr1.2:63720-63739 MS.gene24088:CDS 40.0%
AATTCACCTACCAACAACTC+TGG + chr1.2:61137-61156 MS.gene24088:CDS 40.0%
AGAGAGTCGTAAGTATGGTA+CGG + chr1.2:62032-62051 MS.gene24088:CDS 40.0%
AGCGGTCTTGATTGATAAGA+AGG - chr1.2:63643-63662 None:intergenic 40.0%
AGTCATGCACGTCTAATGAA+TGG - chr1.2:62968-62987 None:intergenic 40.0%
AGTGAAAGCAAGAACAGGTT+TGG - chr1.2:61353-61372 None:intergenic 40.0%
ATTTCGCGAGATGTATTGAG+GGG - chr1.2:61310-61329 None:intergenic 40.0%
CATCGCTATGAGATTCTTGT+GGG - chr1.2:61593-61612 None:intergenic 40.0%
GAAGATACTACTCCTTTGCT+TGG - chr1.2:61571-61590 None:intergenic 40.0%
GATTTCGCGAGATGTATTGA+GGG - chr1.2:61311-61330 None:intergenic 40.0%
GGAAAGACAAAACATCCAGA+TGG + chr1.2:62263-62282 MS.gene24088:intron 40.0%
GTTGGTCATATGCTTGACTT+AGG + chr1.2:62718-62737 MS.gene24088:intron 40.0%
TAGTTGAAGTGATCGAAACG+TGG - chr1.2:63331-63350 None:intergenic 40.0%
TATAAACTCGTGGATCCATC+TGG - chr1.2:62281-62300 None:intergenic 40.0%
TGAAACAGAGGGAGTAATGT+TGG + chr1.2:62700-62719 MS.gene24088:intron 40.0%
TTCAGCATAGCAACCAGTAT+CGG - chr1.2:61829-61848 None:intergenic 40.0%
TTCCAATGGCATATGTGGTA+AGG + chr1.2:63716-63735 MS.gene24088:CDS 40.0%
TTCCTTACCACATATGCCAT+TGG - chr1.2:63721-63740 None:intergenic 40.0%
TTGGAAGAGAGTCGTAAGTA+TGG + chr1.2:62027-62046 MS.gene24088:CDS 40.0%
! ACTGTTTGAGTTCGAACTTG+TGG + chr1.2:62864-62883 MS.gene24088:intron 40.0%
! AGAGTTGTTGGTAGGTGAAT+TGG - chr1.2:61138-61157 None:intergenic 40.0%
! AGGTTATTTCAGTAACGAGG+AGG + chr1.2:63137-63156 MS.gene24088:intron 40.0%
! CGATGGATTCTTATTTGTCG+TGG + chr1.2:63224-63243 MS.gene24088:CDS 40.0%
! CTGTTTGAGTTCGAACTTGT+GGG + chr1.2:62865-62884 MS.gene24088:intron 40.0%
! TATGGATTTTGTTGCAGAGC+AGG + chr1.2:63771-63790 MS.gene24088:CDS 40.0%
!! AGAGGGTTGGTTAAAAACAG+GGG + chr1.2:63182-63201 MS.gene24088:CDS 40.0%
!! AGCGATGGTTCAAGTTGTAA+TGG + chr1.2:61606-61625 MS.gene24088:CDS 40.0%
!! CAGAGGGTTGGTTAAAAACA+GGG + chr1.2:63181-63200 MS.gene24088:CDS 40.0%
!! TGTTGTTGATGATGAGTTGC+TGG - chr1.2:61443-61462 None:intergenic 40.0%
!!! CGACACTCATTTTGAAACAG+AGG + chr1.2:62688-62707 MS.gene24088:intron 40.0%
!!! GACACTCATTTTGAAACGGA+TGG - chr1.2:62447-62466 None:intergenic 40.0%
AACAGGGAAGTAGATGGAGT+TGG - chr1.2:61239-61258 None:intergenic 45.0%
AAGTGGTGGCATCTAAAGAG+TGG - chr1.2:61057-61076 None:intergenic 45.0%
ACAGGGAAGTAGATGGAGTT+GGG - chr1.2:61238-61257 None:intergenic 45.0%
ACATCGACCATTACTCCAGA+GGG + chr1.2:63165-63184 MS.gene24088:CDS 45.0%
ACTTCCTCACTCTCCAACAT+GGG + chr1.2:61178-61197 MS.gene24088:CDS 45.0%
AGAGTGATCGATCCAAGAAG+TGG + chr1.2:60971-60990 MS.gene24088:CDS 45.0%
AGATGTATTGAGGGGATTGG+TGG - chr1.2:61302-61321 None:intergenic 45.0%
AGGTATCCAAATAAGGAGGC+TGG + chr1.2:63688-63707 MS.gene24088:intron 45.0%
AGTAGAAGCTCCAATTCCAG+AGG - chr1.2:62001-62020 None:intergenic 45.0%
CCACAAGAATCTCATAGCGA+TGG + chr1.2:61591-61610 MS.gene24088:CDS 45.0%
CCAGTCCGATTAACTGGTAA+CGG - chr1.2:62114-62133 None:intergenic 45.0%
CCATCGCTATGAGATTCTTG+TGG - chr1.2:61594-61613 None:intergenic 45.0%
CGATTTCGCGAGATGTATTG+AGG - chr1.2:61312-61331 None:intergenic 45.0%
CTCAGCCGTTACCAGTTAAT+CGG + chr1.2:62106-62125 MS.gene24088:CDS 45.0%
CTCTACTCATCAGGAACAAC+TGG + chr1.2:61544-61563 MS.gene24088:CDS 45.0%
CTTGTCAGGAAGCCAAGTTA+TGG + chr1.2:63753-63772 MS.gene24088:CDS 45.0%
GAGTGATCGATCCAAGAAGT+GGG + chr1.2:60972-60991 MS.gene24088:CDS 45.0%
GGAGTAGTGAAAGCAAGAAC+AGG - chr1.2:61358-61377 None:intergenic 45.0%
GGTATCCAAATAAGGAGGCT+GGG + chr1.2:63689-63708 MS.gene24088:intron 45.0%
GTGGCATCTAAAGAGTGGTT+TGG - chr1.2:61052-61071 None:intergenic 45.0%
GTGGTGTCCATGAGAATGAT+TGG - chr1.2:61421-61440 None:intergenic 45.0%
TATTGAACACGAGGTAGACC+CGG + chr1.2:62332-62351 MS.gene24088:intron 45.0%
TCAGTCACTCTACTCAACTC+AGG - chr1.2:61493-61512 None:intergenic 45.0%
TCCTGATGAGTAGAGTAGTG+TGG - chr1.2:61539-61558 None:intergenic 45.0%
TGGAAGTGAAGGTGATGCTT+GGG - chr1.2:61401-61420 None:intergenic 45.0%
TGGAGCTTCTACTGAGTCTT+TGG + chr1.2:62008-62027 MS.gene24088:CDS 45.0%
TTGGAAGTGAAGGTGATGCT+TGG - chr1.2:61402-61421 None:intergenic 45.0%
! GAAAAAGCTTGGTGAGCCTT+TGG + chr1.2:63027-63046 MS.gene24088:intron 45.0%
! GCAGAGCAGGTTTGTTTGTT+TGG + chr1.2:63784-63803 MS.gene24088:intron 45.0%
! GCATCTAAAGAGTGGTTTGG+TGG - chr1.2:61049-61068 None:intergenic 45.0%
! GGCAGGTTATTTCAGTAACG+AGG + chr1.2:63134-63153 MS.gene24088:intron 45.0%
! GTTGTTGGTAGGTGAATTGG+TGG - chr1.2:61135-61154 None:intergenic 45.0%
! TTTGAGTTCGCTTCATACGG+TGG + chr1.2:61879-61898 MS.gene24088:CDS 45.0%
!! AAAGAGTGGTTTGGTGGAAG+TGG - chr1.2:61043-61062 None:intergenic 45.0%
!! AACAAAGCCTCTAGTTCTGC+TGG - chr1.2:63477-63496 None:intergenic 45.0%
!! ATGCAAGACATGGTTGGTGA+TGG + chr1.2:63113-63132 MS.gene24088:intron 45.0%
!! CCAGAGGGTTGGTTAAAAAC+AGG + chr1.2:63180-63199 MS.gene24088:CDS 45.0%
!! GGTCTTGCAGTGTTTGCTAT+GGG + chr1.2:61697-61716 MS.gene24088:CDS 45.0%
!! GTCTTGCAGTGTTTGCTATG+GGG + chr1.2:61698-61717 MS.gene24088:CDS 45.0%
!! TAACCAACCCTCTGGAGTAA+TGG - chr1.2:63175-63194 None:intergenic 45.0%
!! TGTTGATGATGAGTTGCTGG+TGG - chr1.2:61440-61459 None:intergenic 45.0%
!! TTAATCGGACTGGTGAGCTT+TGG + chr1.2:62121-62140 MS.gene24088:CDS 45.0%
!!! CCTGTTTTTAACCAACCCTC+TGG - chr1.2:63183-63202 None:intergenic 45.0%
!! TATAATTAATTTATATTCTT+TGG - chr1.2:63548-63567 None:intergenic 5.0%
!!! ATTAATTTTTTTCAATATAT+AGG + chr1.2:63881-63900 MS.gene24088:intron 5.0%
!!! ATTTATTTTTTATAATATGA+TGG + chr1.2:63600-63619 MS.gene24088:intron 5.0%
ACAGCAGCGTCTACAATAGC+AGG - chr1.2:63510-63529 None:intergenic 50.0%
ACCTTTACGGATCCCCATGT+TGG - chr1.2:61194-61213 None:intergenic 50.0%
AGCTCACCAGTCCGATTAAC+TGG - chr1.2:62120-62139 None:intergenic 50.0%
AGGCGTCAATGAAGGCGATA+TGG - chr1.2:61105-61124 None:intergenic 50.0%
AGTAGATGGAGTTGGGAGAG+AGG - chr1.2:61231-61250 None:intergenic 50.0%
AGTCGTAAGTATGGTACGGC+GGG + chr1.2:62036-62055 MS.gene24088:CDS 50.0%
ATTGTTAGGCAGAGCACACC+AGG - chr1.2:62833-62852 None:intergenic 50.0%
CACATCGACCATTACTCCAG+AGG + chr1.2:63164-63183 MS.gene24088:CDS 50.0%
CACTTCCTCACTCTCCAACA+TGG + chr1.2:61177-61196 MS.gene24088:CDS 50.0%
CCGTTACCAGTTAATCGGAC+TGG + chr1.2:62111-62130 MS.gene24088:CDS 50.0%
CGTGGTCCCACCATTATGAA+AGG + chr1.2:62147-62166 MS.gene24088:CDS 50.0%
CTGCTGCAAAACCCACTTCT+TGG - chr1.2:60986-61005 None:intergenic 50.0%
CTTCCTCACTCTCCAACATG+GGG + chr1.2:61179-61198 MS.gene24088:CDS 50.0%
GAGTCGTAAGTATGGTACGG+CGG + chr1.2:62035-62054 MS.gene24088:CDS 50.0%
GCAGACAACAGGGAAGTAGA+TGG - chr1.2:61245-61264 None:intergenic 50.0%
GCAGGTTCCAATGGCATATG+TGG + chr1.2:63711-63730 MS.gene24088:CDS 50.0%
GCATATGTGGTAAGGAAGGC+TGG + chr1.2:63724-63743 MS.gene24088:CDS 50.0%
GCCACACTACTCTACTCATC+AGG + chr1.2:61535-61554 MS.gene24088:CDS 50.0%
GCGAGATGTATTGAGGGGAT+TGG - chr1.2:61305-61324 None:intergenic 50.0%
GGAACAACTGGACCAAGCAA+AGG + chr1.2:61556-61575 MS.gene24088:CDS 50.0%
GGAGGATGACGTCACCTTTA+CGG - chr1.2:61207-61226 None:intergenic 50.0%
GGATATTGGGGATCGAATCC+TGG + chr1.2:62812-62831 MS.gene24088:intron 50.0%
GTAGTGTGGCTGTATCATCC+TGG - chr1.2:61525-61544 None:intergenic 50.0%
GTCAACAATCATAGCCTCCG+TGG - chr1.2:62076-62095 None:intergenic 50.0%
GTTGGAGAGTGAGGAAGTGA+CGG - chr1.2:61176-61195 None:intergenic 50.0%
TAGACGTGCATGACTCTCCA+AGG + chr1.2:62973-62992 MS.gene24088:intron 50.0%
TCCAACATGGGGATCCGTAA+AGG + chr1.2:61190-61209 MS.gene24088:CDS 50.0%
TCGCTTCATACGGTGGTTTC+TGG + chr1.2:61886-61905 MS.gene24088:CDS 50.0%
TGCTATGGGGCTTCTTGCTA+TGG + chr1.2:61711-61730 MS.gene24088:CDS 50.0%
! AAGGCTGGAAGTAGCTTGTC+AGG + chr1.2:63739-63758 MS.gene24088:CDS 50.0%
! AGCATAGCAACCAGTATCGG+CGG - chr1.2:61826-61845 None:intergenic 50.0%
! CGGTCTTGCAGTGTTTGCTA+TGG + chr1.2:61696-61715 MS.gene24088:CDS 50.0%
! CTTCATACGGTGGTTTCTGG+TGG + chr1.2:61889-61908 MS.gene24088:CDS 50.0%
! GCTATGGGGCTTCTTGCTAT+GGG + chr1.2:61712-61731 MS.gene24088:CDS 50.0%
! GTGAGCCTTTGGTTAGTTGC+TGG + chr1.2:63038-63057 MS.gene24088:intron 50.0%
! TGTATTGAGGGGATTGGTGG+TGG - chr1.2:61299-61318 None:intergenic 50.0%
! TTGGTAGGTGAATTGGTGGC+CGG - chr1.2:61131-61150 None:intergenic 50.0%
!! AAAGCCTCTAGTTCTGCTGG+AGG - chr1.2:63474-63493 None:intergenic 50.0%
!! AAGACATGGTTGGTGATGGC+AGG + chr1.2:63117-63136 MS.gene24088:intron 50.0%
!! AAGCCTCTAGTTCTGCTGGA+GGG - chr1.2:63473-63492 None:intergenic 50.0%
!! AGTGGTTTGGTGGAAGTGGA+AGG - chr1.2:61039-61058 None:intergenic 50.0%
!! CTCTGGAGTAATGGTCGATG+TGG - chr1.2:63166-63185 None:intergenic 50.0%
!! GTGGTTTGGTGGAAGTGGAA+GGG - chr1.2:61038-61057 None:intergenic 50.0%
!! TGGTTTGGTGGAAGTGGAAG+GGG - chr1.2:61037-61056 None:intergenic 50.0%
ACCTGCCCAGCCTCCTTATT+TGG - chr1.2:63697-63716 None:intergenic 55.0%
AGATGGAGTTGGGAGAGAGG+AGG - chr1.2:61228-61247 None:intergenic 55.0%
AGCACGCCAGAGTTGTTGGT+AGG - chr1.2:61146-61165 None:intergenic 55.0%
AGTGACTGAGCGCTTCAACC+AGG + chr1.2:61504-61523 MS.gene24088:CDS 55.0%
CGACCATTACTCCAGAGGGT+TGG + chr1.2:63169-63188 MS.gene24088:CDS 55.0%
CTACAGCACGCCAGAGTTGT+TGG - chr1.2:61150-61169 None:intergenic 55.0%
GATCCCCATGTTGGAGAGTG+AGG - chr1.2:61185-61204 None:intergenic 55.0%
GTCGTAAGTATGGTACGGCG+GGG + chr1.2:62037-62056 MS.gene24088:CDS 55.0%
TCCAAATAAGGAGGCTGGGC+AGG + chr1.2:63693-63712 MS.gene24088:CDS 55.0%
TGACGGAGAGGCAGACAACA+GGG - chr1.2:61255-61274 None:intergenic 55.0%
! ACTGGTGAGCTTTGGCTTCG+TGG + chr1.2:62129-62148 MS.gene24088:CDS 55.0%
! ATGACGGAGAGGCAGACAAC+AGG - chr1.2:61256-61275 None:intergenic 55.0%
ACCACTTGTGCCGCCGATAC+TGG + chr1.2:61813-61832 MS.gene24088:CDS 60.0%
AGCGCGGGAGGAGATGAAAG+TGG - chr1.2:61074-61093 None:intergenic 60.0%
ATCTCCTCCCGCGCTCATCA+CGG + chr1.2:61079-61098 MS.gene24088:CDS 60.0%
CGATATGGCCGTGATGAGCG+CGG - chr1.2:61090-61109 None:intergenic 60.0%
CGGCGGCACAAGTGGTAAGA+AGG - chr1.2:61809-61828 None:intergenic 60.0%
GATATGGCCGTGATGAGCGC+GGG - chr1.2:61089-61108 None:intergenic 60.0%
GCCTTCATTGACGCCTCCAC+CGG + chr1.2:61109-61128 MS.gene24088:CDS 60.0%
GCGGGAGGAGATGAAAGTGG+TGG - chr1.2:61071-61090 None:intergenic 60.0%
GCTTGTGTCTGCTTCCACGG+AGG + chr1.2:62059-62078 MS.gene24088:CDS 60.0%
GGGGCTTGTGTCTGCTTCCA+CGG + chr1.2:62056-62075 MS.gene24088:CDS 60.0%
GGTCCCTCCAGCAGAACTAG+AGG + chr1.2:63467-63486 MS.gene24088:intron 60.0%
TGCCTCTCCGTCATGTCCCT+CGG + chr1.2:61262-61281 MS.gene24088:CDS 60.0%
TGGAGTTGGGAGAGAGGAGG+AGG - chr1.2:61225-61244 None:intergenic 60.0%
! GATAGCGCCGAGGGACATGA+CGG - chr1.2:61272-61291 None:intergenic 60.0%
! GGTGGTGATGATAGCGCCGA+GGG - chr1.2:61281-61300 None:intergenic 60.0%
! GTAGGTGAATTGGTGGCCGG+TGG - chr1.2:61128-61147 None:intergenic 60.0%
! TGGTGGTGATGATAGCGCCG+AGG - chr1.2:61282-61301 None:intergenic 60.0%
!! ACCAGTATCGGCGGCACAAG+TGG - chr1.2:61817-61836 None:intergenic 60.0%
GGAGGCTGGGCAGGTTCCAA+TGG + chr1.2:63702-63721 MS.gene24088:CDS 65.0%
! GGTGAATTGGTGGCCGGTGG+AGG - chr1.2:61125-61144 None:intergenic 65.0%
!! GCCGGTGGAGGCGTCAATGA+AGG - chr1.2:61113-61132 None:intergenic 65.0%
ATGGCCGTGATGAGCGCGGG+AGG - chr1.2:61086-61105 None:intergenic 70.0%
! CGCCGAGGGACATGACGGAG+AGG - chr1.2:61267-61286 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 60938 64016 60938 ID=MS.gene24088
chr1.2 mRNA 60938 64016 60938 ID=MS.gene24088.t1;Parent=MS.gene24088
chr1.2 exon 60938 62168 60938 ID=MS.gene24088.t1.exon1;Parent=MS.gene24088.t1
chr1.2 CDS 60938 62168 60938 ID=cds.MS.gene24088.t1;Parent=MS.gene24088.t1
chr1.2 exon 63139 63284 63139 ID=MS.gene24088.t1.exon2;Parent=MS.gene24088.t1
chr1.2 CDS 63139 63284 63139 ID=cds.MS.gene24088.t1;Parent=MS.gene24088.t1
chr1.2 exon 63468 63535 63468 ID=MS.gene24088.t1.exon3;Parent=MS.gene24088.t1
chr1.2 CDS 63468 63535 63468 ID=cds.MS.gene24088.t1;Parent=MS.gene24088.t1
chr1.2 exon 63690 63792 63690 ID=MS.gene24088.t1.exon4;Parent=MS.gene24088.t1
chr1.2 CDS 63690 63792 63690 ID=cds.MS.gene24088.t1;Parent=MS.gene24088.t1
chr1.2 exon 63903 64016 63903 ID=MS.gene24088.t1.exon5;Parent=MS.gene24088.t1
chr1.2 CDS 63903 64016 63903 ID=cds.MS.gene24088.t1;Parent=MS.gene24088.t1
Gene Sequence

>MS.gene24088

ATGGAGGTAAACGGTGAGAATACGAATAGCAGAAGAGTGATCGATCCAAGAAGTGGGTTTTGCAGCAGCAATTCAATCTTCTACAGCAAGAGAAAACCCCTTCCACTTCCACCAAACCACTCTTTAGATGCCACCACTTTCATCTCCTCCCGCGCTCATCACGGCCATATCGCCTTCATTGACGCCTCCACCGGCCACCAATTCACCTACCAACAACTCTGGCGTGCTGTAGACTCCGTCACTTCCTCACTCTCCAACATGGGGATCCGTAAAGGTGACGTCATCCTCCTCCTCTCTCCCAACTCCATCTACTTCCCTGTTGTCTGCCTCTCCGTCATGTCCCTCGGCGCTATCATCACCACCACCAATCCCCTCAATACATCTCGCGAAATCGCTAAACAGATCGCTGATTCCAAACCTGTTCTTGCTTTCACTACTCCACAACTCCTTTCTAAAATCACCCAAGCATCACCTTCACTTCCAATCATTCTCATGGACACCACCAGCAACTCATCATCAACAACAACACTTGATGAAATGATGAAGAAAGAACCTGAGTTGAGTAGAGTGACTGAGCGCTTCAACCAGGATGATACAGCCACACTACTCTACTCATCAGGAACAACTGGACCAAGCAAAGGAGTAGTATCTTCCCACAAGAATCTCATAGCGATGGTTCAAGTTGTAATGGCTAGATTTGGTAAAGAACAAGAGACGAGAGAAACTTTCATATGCACTGTTCCGATGTTTCATATATACGGTCTTGCAGTGTTTGCTATGGGGCTTCTTGCTATGGGTTCAACCATAGTTGTTCTCTCTAAATTCGAGATGCATGACATGCTTTCATCTATTGAGAAATTCAGAGCAACCTTCTTACCACTTGTGCCGCCGATACTGGTTGCTATGCTGAACAACGCAGATGCGATTAAGAGAAAGTATGATTTGAGTTCGCTTCATACGGTGGTTTCTGGTGGTGCTCCTTTGAGTAAAGAAGTAACGGAAGGGTTTATAGAAAAGTATCCTAATGTAAGTATCTTTCAAGGTTATGGTTTAACTGAATCCTCTGGAATTGGAGCTTCTACTGAGTCTTTGGAAGAGAGTCGTAAGTATGGTACGGCGGGGCTTGTGTCTGCTTCCACGGAGGCTATGATTGTTGACACTGAGACTGCTCAGCCGTTACCAGTTAATCGGACTGGTGAGCTTTGGCTTCGTGGTCCCACCATTATGAAAGGTTCGTTTCTTTTCAAATGTCGTCGCTTTGTTTCTAATTAATTGATCTTTTGATTCTGAAGATCGCTCTTTTAATAAACAATTCAGATTAGATTGGAAAGACAAAACATCCAGATGGATCCACGAGTTTATATGCTATAATTTCCTTTTTTAATTAATTAAAATATTGAACACGAGGTAGACCCGGAAACTTGTTTAGCAATTCAGAGTTTATATATTTGTATTTGTAGTTGATTGATGAGAATGAGAACATGTGGTCTATTGGAATTTAATACTCCATCCGTTTCAAAATGAGTGTCACTTTAAGCAAAAAAATTTGTTTCAAAATGAATGTCGCTTTACATTTCCAATACAACTTTAATTTTTTTCTTCCAACTTTACCCTCTCAAACTTTTCTCACACAATTTTCAATGCAACTTTAAGTTTGTCTTTTTCTAATAAAGTAAGGACAGTTTAGTAAAAGCGTTATATCCAATGATTATTTCATTCCATTTCTTAATCTGTGTGTCAAAGCCTAAAGCGACACTCATTTTGAAACAGAGGGAGTAATGTTGGTCATATGCTTGACTTAGGCTATATATCCCACATTTTTTAGAATATCGATAGACAAAATGACAAAACATTTATTACAAACTCACACAAATGGATATTGGGGATCGAATCCTGGTGTGCTCTGCCTAACAATTTTGACATTTTACTGTTTGAGTTCGAACTTGTGGGCTTTCGCTTTTCTAATTTAATAAATTTAATTTTTATTTAATAAAAAAAGAAGTAACCATTAGACATAAAAAAAGTAACCATTCATTAGACGTGCATGACTCTCCAAGGTGTTTATAACACTAAAATAATAGATGCAAAGGAAAAAGCTTGGTGAGCCTTTGGTTAGTTGCTGGTATTAGATATAAGCTCACATAATTGTTGGTTCTATTATGGATGCTAATTAAAATGCAAGACATGGTTGGTGATGGCAGGTTATTTCAGTAACGAGGAGGCAACCACATCGACCATTACTCCAGAGGGTTGGTTAAAAACAGGGGATGTTTGCTATATTGATAGCGATGGATTCTTATTTGTCGTGGATCGCTTGAAAGAGCTCATTAAGTACAAGGGATATCAGGTTATTATTAACAATTCTATCTAATCTAATGCAGTATTCACAACCACGTTTCGATCACTTCAACTAAATAACCCAGAGATAAATTACTGTTTACCGACACACAATAGTAATAAAGATTATAAACGTAAATATTGTCAAACTCATGGAAGTTCTCTTGCTTTTGTTTTATGGTTATTGTTACAGGTCCCTCCAGCAGAACTAGAGGCTTTGTTGCTGACTCATCCTGCTATTGTAGACGCTGCTGTCATACCGTAAGCCTACCAAAGAATATAAATTAATTATAGTATCTTAATTTTAGTTTTTTTGTCTGCTTTGATTTATTTTTTATAATATGATGGTGACGAAAACCAGTATTCCTTCTTATCAATCAAGACCGCTGAATGTTTTGTGTGTATATTATATCAGGTATCCAAATAAGGAGGCTGGGCAGGTTCCAATGGCATATGTGGTAAGGAAGGCTGGAAGTAGCTTGTCAGGAAGCCAAGTTATGGATTTTGTTGCAGAGCAGGTTTGTTTGTTTGGAACACTTCTCAACAATGACACTCTTTTTTATTAGATGCTTGGGTTTTGATTTCAACTATGAGATTGAAAACTAAATTAATTTTTTTCAATATATAGGTGGCTCCATACAAAAGAATTCGAAAAGTGGCATTTATATCTTCTATACCCAAAAATCCATCTGGTAAAATTCTTCGGAAGGATCTTATCAAGCTAGCTACGTCTAAACTTTGA
Protein sequence

>MS.gene24088.t1

MEVNGENTNSRRVIDPRSGFCSSNSIFYSKRKPLPLPPNHSLDATTFISSRAHHGHIAFIDASTGHQFTYQQLWRAVDSVTSSLSNMGIRKGDVILLLSPNSIYFPVVCLSVMSLGAIITTTNPLNTSREIAKQIADSKPVLAFTTPQLLSKITQASPSLPIILMDTTSNSSSTTTLDEMMKKEPELSRVTERFNQDDTATLLYSSGTTGPSKGVVSSHKNLIAMVQVVMARFGKEQETRETFICTVPMFHIYGLAVFAMGLLAMGSTIVVLSKFEMHDMLSSIEKFRATFLPLVPPILVAMLNNADAIKRKYDLSSLHTVVSGGAPLSKEVTEGFIEKYPNVSIFQGYGLTESSGIGASTESLEESRKYGTAGLVSASTEAMIVDTETAQPLPVNRTGELWLRGPTIMKGYFSNEEATTSTITPEGWLKTGDVCYIDSDGFLFVVDRLKELIKYKGYQVPPAELEALLLTHPAIVDAAVIPYPNKEAGQVPMAYVVRKAGSSLSGSQVMDFVAEQVAPYKRIRKVAFISSIPKNPSGKILRKDLIKLATSKL