AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88987

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88987.t1 MTR_1g026310 95.536 560 18 1 1 553 1 560 0.0 1096
MS.gene88987.t1 MTR_8g039720 80.071 567 91 6 1 553 1 559 0.0 906
MS.gene88987.t1 MTR_1g026470 94.802 404 14 1 1 397 1 404 0.0 779
MS.gene88987.t1 MTR_1g026430 95.511 401 11 1 1 394 1 401 0.0 779
MS.gene88987.t1 MTR_4g057730 45.672 543 286 5 17 553 3 542 1.99e-161 471
MS.gene88987.t1 MTR_3g088870 45.307 554 270 11 14 549 16 554 1.01e-159 467
MS.gene88987.t1 MTR_5g027480 43.571 560 304 6 1 553 1 555 5.69e-158 462
MS.gene88987.t1 MTR_1g090737 46.875 544 271 8 15 546 22 559 1.62e-157 462
MS.gene88987.t1 MTR_3g088880 44.464 560 277 12 7 549 10 552 5.25e-157 460
MS.gene88987.t1 MTR_2g095510 43.693 547 288 6 17 553 3 539 1.59e-152 448
MS.gene88987.t1 MTR_5g027500 44.790 547 278 9 26 553 27 568 2.40e-151 446
MS.gene88987.t1 MTR_4g077900 46.266 482 247 5 80 553 1 478 1.04e-150 441
MS.gene88987.t1 MTR_1g063110 43.808 541 288 8 27 553 13 551 1.85e-144 428
MS.gene88987.t1 MTR_1g063120 43.486 545 288 8 27 553 13 555 1.39e-142 423
MS.gene88987.t1 MTR_1g073180 44.096 542 279 10 14 546 1 527 2.71e-139 414
MS.gene88987.t1 MTR_4g057730 44.068 472 255 5 17 482 3 471 1.01e-129 387
MS.gene88987.t1 MTR_1g073180 42.830 530 276 11 8 528 20 531 2.17e-125 378
MS.gene88987.t1 MTR_2g105570 40.075 534 302 8 26 545 12 541 6.02e-120 365
MS.gene88987.t1 MTR_4g057730 43.111 450 247 5 17 460 3 449 2.20e-118 357
MS.gene88987.t1 MTR_4g128337 38.403 526 316 4 26 545 17 540 1.51e-117 358
MS.gene88987.t1 MTR_3g460770 35.075 536 324 7 20 543 8 531 1.17e-115 354
MS.gene88987.t1 MTR_5g007640 38.649 533 306 7 26 545 55 579 1.77e-115 354
MS.gene88987.t1 MTR_4g005750 40.152 528 304 8 26 545 11 534 3.57e-113 347
MS.gene88987.t1 MTR_2g095510 40.749 454 249 6 17 460 3 446 8.16e-108 330
MS.gene88987.t1 MTR_1g026350 94.118 153 9 0 1 153 1 153 1.04e-100 301
MS.gene88987.t1 MTR_1569s0010 28.215 521 316 12 68 545 22 527 1.32e-54 193
MS.gene88987.t1 MTR_3g035130 28.684 509 336 13 44 543 15 505 2.95e-51 184
MS.gene88987.t1 MTR_8g074040 28.431 510 314 16 67 542 81 573 7.27e-47 173
MS.gene88987.t1 MTR_3g064420 26.908 524 326 13 65 545 37 546 2.82e-45 168
MS.gene88987.t1 MTR_5g008800 27.621 496 326 12 68 539 40 526 1.23e-43 163
MS.gene88987.t1 MTR_5g076240 26.755 527 319 13 68 549 40 544 3.75e-42 159
MS.gene88987.t1 MTR_3g035130 28.947 456 297 13 44 490 15 452 1.71e-41 155
MS.gene88987.t1 MTR_8g074040 28.633 461 278 16 116 542 12 455 2.01e-40 153
MS.gene88987.t1 MTR_4g013210 29.563 389 238 11 192 552 65 445 2.49e-39 149
MS.gene88987.t1 MTR_5g008820 25.605 496 334 13 68 539 40 524 9.03e-39 149
MS.gene88987.t1 MTR_0803s0020 25.979 485 337 11 67 539 260 734 1.21e-38 152
MS.gene88987.t1 MTR_8g074120 27.101 476 302 15 98 542 23 484 1.97e-37 145
MS.gene88987.t1 MTR_2g044180 24.723 542 359 17 49 553 29 558 1.40e-36 143
MS.gene88987.t1 MTR_5g076220 25.528 521 334 11 68 549 40 545 1.96e-36 143
MS.gene88987.t1 MTR_4g011790 26.440 382 246 9 197 552 226 598 2.39e-36 143
MS.gene88987.t1 MTR_5g076200 25.434 519 337 11 68 549 40 545 2.61e-36 142
MS.gene88987.t1 MTR_4g121600 24.859 531 346 15 67 553 45 566 4.66e-35 139
MS.gene88987.t1 MTR_0161s0050 26.232 568 355 16 24 549 2 547 1.36e-34 138
MS.gene88987.t1 MTR_4g011790 27.110 391 241 12 197 552 226 607 1.81e-34 138
MS.gene88987.t1 MTR_4g011790 25.946 370 239 9 197 540 226 586 9.21e-34 136
MS.gene88987.t1 MTR_7g084850 23.092 524 363 11 68 553 203 724 1.58e-33 136
MS.gene88987.t1 MTR_2g044180 27.660 376 246 12 195 553 101 467 2.42e-33 133
MS.gene88987.t1 MTR_4g013210 24.660 515 327 17 52 521 68 566 5.39e-33 133
MS.gene88987.t1 MTR_4g013210 29.521 376 214 14 192 552 65 404 2.14e-31 126
MS.gene88987.t1 MTR_5g023730 24.606 508 335 16 67 542 42 533 3.36e-31 127
MS.gene88987.t1 MTR_4g013210 25.612 531 323 21 52 552 68 556 4.58e-31 127
MS.gene88987.t1 MTR_8g083170 25.919 571 301 18 69 528 110 669 4.03e-29 122
MS.gene88987.t1 MTR_3g084470 25.989 531 275 18 69 491 106 626 9.09e-28 118
MS.gene88987.t1 MTR_2g030240 25.282 443 299 11 69 485 29 465 3.97e-27 115
MS.gene88987.t1 MTR_2g030240 27.243 301 196 7 202 485 77 371 5.31e-24 105
MS.gene88987.t1 MTR_3g088890 60.938 64 25 0 455 518 10 73 1.74e-22 92.4
MS.gene88987.t1 MTR_1g104810 25.109 458 256 16 62 466 141 564 1.68e-21 99.0
MS.gene88987.t1 MTR_1g104810 25.328 458 255 15 62 466 74 497 2.60e-21 98.2
MS.gene88987.t1 MTR_4g057755 57.534 73 31 0 409 481 46 118 2.81e-21 90.1
MS.gene88987.t1 MTR_1054s0020 23.529 493 347 16 65 547 294 766 8.10e-21 97.1
MS.gene88987.t1 MTR_3g087980 27.697 343 174 15 198 475 248 581 3.79e-20 94.7
MS.gene88987.t1 MTR_1g086600 25.749 334 192 11 198 480 243 571 1.21e-19 93.2
MS.gene88987.t1 MTR_1g030290 28.235 340 176 13 198 475 250 583 2.24e-19 92.4
MS.gene88987.t1 MTR_4g066130 25.734 443 268 17 158 552 619 1048 4.97e-19 92.0
MS.gene88987.t1 MTR_2g098490 24.943 441 266 17 158 547 614 1040 1.02e-18 90.9
MS.gene88987.t1 MTR_8g099420 24.850 334 187 12 196 475 222 545 2.24e-18 89.4
MS.gene88987.t1 MTR_4g013210 24.429 438 271 18 52 470 68 464 9.46e-17 83.6
MS.gene88987.t1 MTR_3g084450 33.503 197 114 5 322 505 246 438 1.38e-16 83.2
MS.gene88987.t1 MTR_3g084450 33.503 197 114 5 322 505 454 646 2.93e-16 82.8
MS.gene88987.t1 MTR_7g109830 34.756 164 99 6 321 480 388 547 5.94e-15 78.6
MS.gene88987.t1 MTR_4g068670 22.626 495 295 19 62 485 72 549 3.64e-14 75.9
MS.gene88987.t1 MTR_5g009350 22.659 331 200 10 195 475 220 544 8.29e-14 74.7
MS.gene88987.t1 MTR_5g009350 22.659 331 200 10 195 475 220 544 9.24e-14 74.3
MS.gene88987.t1 MTR_5g009350 23.030 330 198 10 196 475 221 544 1.06e-13 74.3
MS.gene88987.t1 MTR_5g009350 21.905 420 250 14 196 546 221 631 1.19e-13 74.3
MS.gene88987.t1 MTR_4g013210 22.581 403 260 15 52 419 68 453 1.78e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88987.t1 AT1G20510 74.630 540 134 2 14 551 4 542 0.0 827
MS.gene88987.t1 AT1G20500 66.302 549 170 5 13 553 9 550 0.0 737
MS.gene88987.t1 AT1G20510 73.673 471 121 2 14 482 4 473 0.0 710
MS.gene88987.t1 AT1G20480 61.429 560 202 4 4 553 10 565 0.0 706
MS.gene88987.t1 AT5G38120 61.580 557 193 7 9 553 3 550 0.0 691
MS.gene88987.t1 AT1G20510 73.051 449 118 2 14 460 4 451 0.0 673
MS.gene88987.t1 AT5G38120 60.269 521 186 7 9 517 3 514 0.0 633
MS.gene88987.t1 AT5G38120 60.494 486 171 7 9 482 3 479 0.0 589
MS.gene88987.t1 AT5G38120 60.494 486 171 7 9 482 3 479 0.0 589
MS.gene88987.t1 AT1G20490 60.047 428 154 5 13 430 9 429 7.89e-179 511
MS.gene88987.t1 AT1G20490 60.047 428 154 5 13 430 9 429 7.89e-179 511
MS.gene88987.t1 AT5G63380 43.162 563 286 8 8 549 5 554 1.10e-164 480
MS.gene88987.t1 AT4G19010 41.591 553 299 7 15 553 20 562 2.76e-155 456
MS.gene88987.t1 AT4G05160 43.876 547 292 7 17 553 3 544 6.96e-155 454
MS.gene88987.t1 AT3G21240 39.474 532 313 6 21 545 17 546 1.55e-120 367
MS.gene88987.t1 AT1G65060 40.643 529 299 6 26 545 34 556 6.62e-120 365
MS.gene88987.t1 AT1G51680 39.377 546 317 6 12 545 10 553 3.88e-112 345
MS.gene88987.t1 AT3G21230 38.632 541 311 9 22 545 24 560 2.04e-110 341
MS.gene88987.t1 AT1G62940 35.619 525 329 4 26 545 12 532 3.47e-102 319
MS.gene88987.t1 AT1G51680 37.452 518 310 6 12 517 10 525 2.06e-97 306
MS.gene88987.t1 AT3G21230 37.115 520 302 10 22 524 24 535 3.13e-97 306
MS.gene88987.t1 AT3G21240 37.500 472 286 6 21 485 17 486 6.95e-96 301
MS.gene88987.t1 AT3G21240 37.500 472 286 6 21 485 17 486 6.95e-96 301
MS.gene88987.t1 AT3G21230 37.107 477 279 9 22 481 24 496 3.20e-88 281
MS.gene88987.t1 AT1G51680 37.137 482 289 6 12 481 10 489 1.68e-87 279
MS.gene88987.t1 AT1G65060 37.220 446 265 6 26 462 34 473 3.95e-81 263
MS.gene88987.t1 AT3G21230 32.678 407 253 9 22 411 24 426 1.43e-51 183
MS.gene88987.t1 AT3G48990 28.008 482 335 10 67 543 30 504 4.92e-47 172
MS.gene88987.t1 AT1G65890 28.060 531 317 18 67 549 39 552 1.32e-46 172
MS.gene88987.t1 AT1G21530 27.788 529 319 16 62 549 37 543 2.45e-46 171
MS.gene88987.t1 AT1G77240 26.705 528 337 15 58 551 32 543 4.07e-46 170
MS.gene88987.t1 AT1G20560 27.188 537 348 14 34 542 14 535 4.40e-46 170
MS.gene88987.t1 AT1G65880 28.652 534 311 19 67 549 39 553 6.11e-46 170
MS.gene88987.t1 AT1G21530 27.514 527 325 15 62 549 37 545 6.39e-46 169
MS.gene88987.t1 AT1G21540 27.938 519 322 14 69 551 46 548 1.79e-45 168
MS.gene88987.t1 AT1G66120 28.000 525 322 15 67 551 39 547 8.97e-45 167
MS.gene88987.t1 AT1G76290 28.101 516 318 18 67 549 39 534 7.10e-44 164
MS.gene88987.t1 AT5G16370 26.628 522 326 14 74 553 43 549 1.76e-41 157
MS.gene88987.t1 AT2G17650 27.013 559 346 19 37 549 55 597 3.16e-40 154
MS.gene88987.t1 AT5G16340 26.796 515 321 14 74 547 43 542 1.54e-39 152
MS.gene88987.t1 AT1G20560 27.692 455 292 12 116 542 12 457 2.80e-39 150
MS.gene88987.t1 AT1G30520 27.155 464 292 11 64 496 34 482 1.77e-38 149
MS.gene88987.t1 AT1G30520 27.155 464 292 11 64 496 34 482 1.77e-38 149
MS.gene88987.t1 AT1G75960 26.488 521 324 14 68 549 41 541 4.86e-37 144
MS.gene88987.t1 AT1G68270 24.952 521 308 16 67 545 39 518 8.05e-34 135
MS.gene88987.t1 AT3G16170 24.672 381 245 9 197 547 234 602 2.89e-32 131
MS.gene88987.t1 AT3G16910 23.269 520 346 16 67 545 47 554 7.93e-32 130
MS.gene88987.t1 AT5G36880 22.761 536 362 13 60 553 144 669 1.09e-31 130
MS.gene88987.t1 AT5G36880 22.761 536 362 13 60 553 144 669 1.09e-31 130
MS.gene88987.t1 AT5G36880 22.761 536 362 13 60 553 144 669 1.09e-31 130
MS.gene88987.t1 AT5G36880 22.761 536 362 13 60 553 144 669 1.09e-31 130
MS.gene88987.t1 AT5G36880 22.948 536 361 14 60 553 194 719 1.49e-31 130
MS.gene88987.t1 AT1G30520 25.843 445 281 12 64 477 34 460 5.88e-31 126
MS.gene88987.t1 AT1G30520 25.843 445 281 12 64 477 34 460 5.88e-31 126
MS.gene88987.t1 AT5G36880 21.957 460 312 11 60 481 144 594 1.37e-24 108
MS.gene88987.t1 AT5G27600 27.760 317 172 9 197 466 261 567 2.08e-22 102
MS.gene88987.t1 AT3G05970 28.707 317 169 9 197 466 261 567 5.42e-20 94.4
MS.gene88987.t1 AT2G47240 26.857 350 199 12 180 480 147 488 1.19e-19 93.2
MS.gene88987.t1 AT2G47240 26.857 350 199 12 180 480 206 547 1.23e-19 93.2
MS.gene88987.t1 AT2G47240 26.857 350 199 12 180 480 206 547 1.23e-19 93.2
MS.gene88987.t1 AT2G47240 26.857 350 199 12 180 480 206 547 1.23e-19 93.2
MS.gene88987.t1 AT1G64400 22.515 493 303 19 55 478 65 547 1.52e-19 92.8
MS.gene88987.t1 AT1G77590 27.874 348 167 14 198 475 100 433 2.74e-19 91.7
MS.gene88987.t1 AT1G77590 23.881 536 297 21 23 475 72 579 3.92e-19 91.7
MS.gene88987.t1 AT1G49430 25.000 344 199 13 193 485 219 554 1.46e-16 83.6
MS.gene88987.t1 AT4G11030 23.548 310 174 10 198 454 224 523 6.31e-15 78.2
MS.gene88987.t1 AT3G23790 32.632 190 103 6 322 490 455 640 3.41e-13 72.8
MS.gene88987.t1 AT3G23790 24.390 246 153 9 68 282 111 354 9.33e-12 68.2
MS.gene88987.t1 AT4G14070 29.200 250 146 8 322 546 471 714 5.57e-12 68.9

Find 158 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGCCCAGCCTCCTTATT+TGG 0.136723 1.4:+462964 None:intergenic
TCGCTTCATACGGTGGTTTC+TGG 0.138183 1.4:-464772 MS.gene88987:CDS
CTGCTGCAAAACCCACTTCT+TGG 0.251921 1.4:+465675 None:intergenic
ACCACTTGTGCCGCCGATAC+TGG 0.271545 1.4:-464845 MS.gene88987:CDS
GTAAGTATCTTTCAAGGTTA+TGG 0.275288 1.4:-464694 MS.gene88987:CDS
TGGAGCTTCTACTGAGTCTT+TGG 0.282200 1.4:-464650 MS.gene88987:CDS
TGTTGTTGATGATGAGTTGC+TGG 0.335506 1.4:+465218 None:intergenic
GTTCCGATGTTTCATATATA+CGG 0.342157 1.4:-464982 MS.gene88987:CDS
TGCTATGGGGCTTCTTGCTA+TGG 0.342915 1.4:-464947 MS.gene88987:CDS
TTAACTGAATCCTCTGGAAT+TGG 0.351281 1.4:-464670 MS.gene88987:CDS
AGCTCACCAGTCCGATTAAC+TGG 0.354985 1.4:+464541 None:intergenic
CTCTACTCATCAGGAACAAC+TGG 0.359656 1.4:-465114 MS.gene88987:CDS
CAAGTTGTAATGGCTAGATT+TGG 0.372468 1.4:-465042 MS.gene88987:CDS
GGAGGATGACGTCACCTTTA+CGG 0.372564 1.4:+465454 None:intergenic
TTATATCAGGTATCCAAATA+AGG 0.373402 1.4:-462977 MS.gene88987:intron
CATTAAGTACAAGGGATATC+AGG 0.379179 1.4:-463395 MS.gene88987:intron
GGTGAATTGGTGGCCGGTGG+AGG 0.402061 1.4:+465536 None:intergenic
ATGACGGAGAGGCAGACAAC+AGG 0.402334 1.4:+465405 None:intergenic
AAGGCTGGAAGTAGCTTGTC+AGG 0.410549 1.4:-462919 MS.gene88987:CDS
TTAATCGGACTGGTGAGCTT+TGG 0.414553 1.4:-464537 MS.gene88987:CDS
TGGAGTTGGGAGAGAGGAGG+AGG 0.419067 1.4:+465436 None:intergenic
GAATTTAGAGAGAACAACTA+TGG 0.419281 1.4:+464918 None:intergenic
AAGAAACGAACCTTTCATAA+TGG 0.419398 1.4:+464501 None:intergenic
AGTAGATGGAGTTGGGAGAG+AGG 0.420482 1.4:+465430 None:intergenic
TGGTTTGGTGGAAGTGGAAG+GGG 0.421746 1.4:+465624 None:intergenic
ACTGGTGAGCTTTGGCTTCG+TGG 0.421933 1.4:-464529 MS.gene88987:CDS
AGTGAAAGCAAGAACAGGTT+TGG 0.423538 1.4:+465308 None:intergenic
ATCCATCTGGTAAAATTCTT+CGG 0.423771 1.4:-462700 MS.gene88987:CDS
AAAGAGTGGTTTGGTGGAAG+TGG 0.424298 1.4:+465618 None:intergenic
CCTTGAAAGATACTTACATT+AGG 0.428705 1.4:+464700 None:intergenic
TCCAAATAAGGAGGCTGGGC+AGG 0.431867 1.4:-462965 MS.gene88987:CDS
CCTAATGTAAGTATCTTTCA+AGG 0.432845 1.4:-464700 MS.gene88987:CDS
CTCAGCCGTTACCAGTTAAT+CGG 0.436385 1.4:-464552 MS.gene88987:CDS
GGTCTTGCAGTGTTTGCTAT+GGG 0.437424 1.4:-464961 MS.gene88987:CDS
CGGTCTTGCAGTGTTTGCTA+TGG 0.438320 1.4:-464962 MS.gene88987:CDS
GCTATGGGGCTTCTTGCTAT+GGG 0.440856 1.4:-464946 MS.gene88987:CDS
CTACAGCACGCCAGAGTTGT+TGG 0.441513 1.4:+465511 None:intergenic
GCAGACAACAGGGAAGTAGA+TGG 0.455653 1.4:+465416 None:intergenic
CTTGTCAGGAAGCCAAGTTA+TGG 0.458124 1.4:-462905 MS.gene88987:CDS
TTGGAAGAGAGTCGTAAGTA+TGG 0.463115 1.4:-464631 MS.gene88987:CDS
AGGCGTCAATGAAGGCGATA+TGG 0.463838 1.4:+465556 None:intergenic
GGGGCTTGTGTCTGCTTCCA+CGG 0.471317 1.4:-464602 MS.gene88987:CDS
TATGGTTTAACTGAATCCTC+TGG 0.471540 1.4:-464676 MS.gene88987:CDS
GTGGTTTGGTGGAAGTGGAA+GGG 0.473586 1.4:+465623 None:intergenic
AGAGTTGTTGGTAGGTGAAT+TGG 0.474858 1.4:+465523 None:intergenic
AGGTATCCAAATAAGGAGGC+TGG 0.481674 1.4:-462970 MS.gene88987:CDS
TTCACTTCCAATCATTCTCA+TGG 0.482486 1.4:-465247 MS.gene88987:CDS
TAACCAACCCTCTGGAGTAA+TGG 0.484888 1.4:+463486 None:intergenic
CGATTTCGCGAGATGTATTG+AGG 0.485277 1.4:+465349 None:intergenic
GCGAGATGTATTGAGGGGAT+TGG 0.486320 1.4:+465356 None:intergenic
AACAAAGCCTCTAGTTCTGC+TGG 0.486762 1.4:+463184 None:intergenic
CACTTCCTCACTCTCCAACA+TGG 0.488973 1.4:-465481 MS.gene88987:CDS
GCCGGTGGAGGCGTCAATGA+AGG 0.490250 1.4:+465548 None:intergenic
AGTGGTTTGGTGGAAGTGGA+AGG 0.490859 1.4:+465622 None:intergenic
GCATATGTGGTAAGGAAGGC+TGG 0.491572 1.4:-462934 MS.gene88987:CDS
ATTAAGAAGATGGAGGTAAA+CGG 0.492454 1.4:-465729 None:intergenic
TGTATTGAGGGGATTGGTGG+TGG 0.493354 1.4:+465362 None:intergenic
CGATGGATTCTTATTTGTCG+TGG 0.494381 1.4:-463434 MS.gene88987:CDS
GTGGTGTCCATGAGAATGAT+TGG 0.496058 1.4:+465240 None:intergenic
CTGCAACAAAATCCATAACT+TGG 0.502133 1.4:+462893 None:intergenic
ACAGGGAAGTAGATGGAGTT+GGG 0.504299 1.4:+465423 None:intergenic
TGATTTGAGTTCGCTTCATA+CGG 0.505643 1.4:-464782 MS.gene88987:CDS
TTGGTAGGTGAATTGGTGGC+CGG 0.506146 1.4:+465530 None:intergenic
GCCACACTACTCTACTCATC+AGG 0.506955 1.4:-465123 MS.gene88987:CDS
AGTCGTAAGTATGGTACGGC+GGG 0.509532 1.4:-464622 MS.gene88987:CDS
AACAGGGAAGTAGATGGAGT+TGG 0.510251 1.4:+465422 None:intergenic
CGGCGGCACAAGTGGTAAGA+AGG 0.513016 1.4:+464852 None:intergenic
CATCGCTATGAGATTCTTGT+GGG 0.513470 1.4:+465068 None:intergenic
GCCTTCATTGACGCCTCCAC+CGG 0.515250 1.4:-465549 MS.gene88987:CDS
TGGAAGTGAAGGTGATGCTT+GGG 0.520153 1.4:+465260 None:intergenic
GGAGTAGTGAAAGCAAGAAC+AGG 0.520555 1.4:+465303 None:intergenic
GAAGATACTACTCCTTTGCT+TGG 0.520592 1.4:+465090 None:intergenic
ACTTCCTCACTCTCCAACAT+GGG 0.520727 1.4:-465480 MS.gene88987:CDS
TGCCTCTCCGTCATGTCCCT+CGG 0.521898 1.4:-465396 MS.gene88987:CDS
TTCCTTACCACATATGCCAT+TGG 0.522529 1.4:+462940 None:intergenic
AGCGATGGTTCAAGTTGTAA+TGG 0.525827 1.4:-465052 MS.gene88987:CDS
TCCGTTACTTCTTTACTCAA+AGG 0.528090 1.4:+464742 None:intergenic
GTTTGCTATATTGATAGCGA+TGG 0.538596 1.4:-463451 MS.gene88987:CDS
TTGGAAGTGAAGGTGATGCT+TGG 0.539417 1.4:+465259 None:intergenic
ATACAAAAGAATTCGAAAAG+TGG 0.543360 1.4:-462747 MS.gene88987:CDS
TTCCAATGGCATATGTGGTA+AGG 0.544112 1.4:-462942 MS.gene88987:CDS
CTTCATACGGTGGTTTCTGG+TGG 0.547015 1.4:-464769 MS.gene88987:CDS
ATCTGGTAAAATTCTTCGGA+AGG 0.547613 1.4:-462696 MS.gene88987:CDS
GGAGGCTGGGCAGGTTCCAA+TGG 0.547827 1.4:-462956 MS.gene88987:CDS
ACAGCAGCGTCTACAATAGC+AGG 0.550475 1.4:+463151 None:intergenic
GTAGGTGAATTGGTGGCCGG+TGG 0.552038 1.4:+465533 None:intergenic
ACCTTTACGGATCCCCATGT+TGG 0.552224 1.4:+465467 None:intergenic
GAAAGAGCTCATTAAGTACA+AGG 0.552506 1.4:-463404 MS.gene88987:CDS
GCATCTAAAGAGTGGTTTGG+TGG 0.553390 1.4:+465612 None:intergenic
CGTGGTCCCACCATTATGAA+AGG 0.554145 1.4:-464511 MS.gene88987:intron
TCCAACATGGGGATCCGTAA+AGG 0.554642 1.4:-465468 MS.gene88987:CDS
GCAGGTTCCAATGGCATATG+TGG 0.556198 1.4:-462947 MS.gene88987:CDS
ACCAGTATCGGCGGCACAAG+TGG 0.558595 1.4:+464844 None:intergenic
AAACGAACCTTTCATAATGG+TGG 0.559397 1.4:+464504 None:intergenic
TTCAGCATAGCAACCAGTAT+CGG 0.561145 1.4:+464832 None:intergenic
CCAGTCCGATTAACTGGTAA+CGG 0.565679 1.4:+464547 None:intergenic
GGTATCCAAATAAGGAGGCT+GGG 0.569081 1.4:-462969 MS.gene88987:CDS
CCATCGCTATGAGATTCTTG+TGG 0.569419 1.4:+465067 None:intergenic
GTGGCATCTAAAGAGTGGTT+TGG 0.570177 1.4:+465609 None:intergenic
CCGTTACCAGTTAATCGGAC+TGG 0.573347 1.4:-464547 MS.gene88987:CDS
TTGAGTAAAGAAGTAACGGA+AGG 0.574145 1.4:-464739 MS.gene88987:CDS
AGATGTATTGAGGGGATTGG+TGG 0.576476 1.4:+465359 None:intergenic
ATCTCCTCCCGCGCTCATCA+CGG 0.577531 1.4:-465579 MS.gene88987:CDS
TCCTTTGAGTAAAGAAGTAA+CGG 0.584099 1.4:-464743 MS.gene88987:CDS
GATTTCGCGAGATGTATTGA+GGG 0.584385 1.4:+465350 None:intergenic
AATTCACCTACCAACAACTC+TGG 0.585675 1.4:-465521 MS.gene88987:CDS
AACGAACCTTTCATAATGGT+GGG 0.587290 1.4:+464505 None:intergenic
GATATGGCCGTGATGAGCGC+GGG 0.587698 1.4:+465572 None:intergenic
AGATGGAGTTGGGAGAGAGG+AGG 0.592217 1.4:+465433 None:intergenic
TGAGTAAAGAAGTAACGGAA+GGG 0.592240 1.4:-464738 MS.gene88987:CDS
GTTGTTGGTAGGTGAATTGG+TGG 0.596032 1.4:+465526 None:intergenic
AATGGCATATGTGGTAAGGA+AGG 0.598196 1.4:-462938 MS.gene88987:CDS
GTCTTGCAGTGTTTGCTATG+GGG 0.598502 1.4:-464960 MS.gene88987:CDS
CGATATGGCCGTGATGAGCG+CGG 0.598855 1.4:+465571 None:intergenic
TGACGGAGAGGCAGACAACA+GGG 0.598859 1.4:+465406 None:intergenic
GTTGGAGAGTGAGGAAGTGA+CGG 0.598886 1.4:+465485 None:intergenic
CGACCATTACTCCAGAGGGT+TGG 0.603546 1.4:-463489 MS.gene88987:CDS
GATAGCGCCGAGGGACATGA+CGG 0.604148 1.4:+465389 None:intergenic
AGAGTGATCGATCCAAGAAG+TGG 0.604701 1.4:-465687 MS.gene88987:CDS
AGTGACTGAGCGCTTCAACC+AGG 0.605836 1.4:-465154 MS.gene88987:CDS
TGTTGATGATGAGTTGCTGG+TGG 0.609037 1.4:+465221 None:intergenic
ATGGCCGTGATGAGCGCGGG+AGG 0.610511 1.4:+465575 None:intergenic
CCACAAGAATCTCATAGCGA+TGG 0.611191 1.4:-465067 MS.gene88987:CDS
GGTCCCTCCAGCAGAACTAG+AGG 0.614140 1.4:-463191 MS.gene88987:CDS
TTTGAGTTCGCTTCATACGG+TGG 0.616174 1.4:-464779 MS.gene88987:CDS
GATCCCCATGTTGGAGAGTG+AGG 0.617341 1.4:+465476 None:intergenic
GGTGGTGATGATAGCGCCGA+GGG 0.619275 1.4:+465380 None:intergenic
AGAGAGTCGTAAGTATGGTA+CGG 0.623595 1.4:-464626 MS.gene88987:CDS
AGCGCGGGAGGAGATGAAAG+TGG 0.628660 1.4:+465587 None:intergenic
GGAACAACTGGACCAAGCAA+AGG 0.634924 1.4:-465102 MS.gene88987:CDS
TCAGTCACTCTACTCAACTC+AGG 0.638010 1.4:+465168 None:intergenic
GTAGTGTGGCTGTATCATCC+TGG 0.639019 1.4:+465136 None:intergenic
ATGAGAATGATTGGAAGTGA+AGG 0.639263 1.4:+465249 None:intergenic
AAAGCCTCTAGTTCTGCTGG+AGG 0.643862 1.4:+463187 None:intergenic
AGCATAGCAACCAGTATCGG+CGG 0.644393 1.4:+464835 None:intergenic
GGCAGGTTATTTCAGTAACG+AGG 0.647450 1.4:-463524 MS.gene88987:intron
ACATCGACCATTACTCCAGA+GGG 0.647619 1.4:-463493 MS.gene88987:CDS
AAAGAGCTCATTAAGTACAA+GGG 0.651964 1.4:-463403 MS.gene88987:CDS
GAGTGATCGATCCAAGAAGT+GGG 0.652473 1.4:-465686 MS.gene88987:CDS
AGACCGTATATATGAAACAT+CGG 0.653796 1.4:+464979 None:intergenic
GCTTGTGTCTGCTTCCACGG+AGG 0.655096 1.4:-464599 MS.gene88987:CDS
CTCTGGAGTAATGGTCGATG+TGG 0.656546 1.4:+463495 None:intergenic
AAGCCTCTAGTTCTGCTGGA+GGG 0.656701 1.4:+463188 None:intergenic
ATTTCGCGAGATGTATTGAG+GGG 0.663874 1.4:+465351 None:intergenic
CTTCCTCACTCTCCAACATG+GGG 0.667378 1.4:-465479 MS.gene88987:CDS
GCGGGAGGAGATGAAAGTGG+TGG 0.667975 1.4:+465590 None:intergenic
AGGTTATTTCAGTAACGAGG+AGG 0.670840 1.4:-463521 MS.gene88987:CDS
CGCCGAGGGACATGACGGAG+AGG 0.671164 1.4:+465394 None:intergenic
AAGTGGTGGCATCTAAAGAG+TGG 0.673146 1.4:+465604 None:intergenic
TATCAGGTATCCAAATAAGG+AGG 0.676684 1.4:-462974 MS.gene88987:intron
CACATCGACCATTACTCCAG+AGG 0.683319 1.4:-463494 MS.gene88987:CDS
TCCTGATGAGTAGAGTAGTG+TGG 0.691172 1.4:+465122 None:intergenic
GTCGTAAGTATGGTACGGCG+GGG 0.691371 1.4:-464621 MS.gene88987:CDS
AGCACGCCAGAGTTGTTGGT+AGG 0.696936 1.4:+465515 None:intergenic
AGTAGAAGCTCCAATTCCAG+AGG 0.697004 1.4:+464660 None:intergenic
TGGTGGTGATGATAGCGCCG+AGG 0.700534 1.4:+465379 None:intergenic
GTCAACAATCATAGCCTCCG+TGG 0.700669 1.4:+464585 None:intergenic
GAGTCGTAAGTATGGTACGG+CGG 0.704214 1.4:-464623 MS.gene88987:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTAATTAATTAAAAA+AGG + chr1.4:464041-464060 None:intergenic 0.0%
!! ATTAATTTATATTCTTTGGT+AGG + chr1.4:465270-465289 None:intergenic 15.0%
!! GAAAAAAATTAAAGTTGTAT+TGG + chr1.4:464243-464262 None:intergenic 15.0%
!!! AATTTTTTTCAATATATAGG+TGG - chr1.4:465610-465629 MS.gene88987:CDS 15.0%
!! AAATGGAATGAAATAATCAT+TGG + chr1.4:464368-464387 None:intergenic 20.0%
!! AGTAAAATGTCAAAATTGTT+AGG + chr1.4:464573-464592 None:intergenic 20.0%
!! ATAAACAATTCAGATTAGAT+TGG - chr1.4:463968-463987 MS.gene88987:intron 20.0%
!! GTAAACAGTAATTTATCTTT+GGG + chr1.4:465085-465104 None:intergenic 20.0%
!! TAATTAAAATATTGAACACG+AGG - chr1.4:464049-464068 MS.gene88987:intron 20.0%
!!! ATCGATATTCTAAAAAATGT+GGG + chr1.4:464478-464497 None:intergenic 20.0%
!!! GTTACTTTTTTTATGTCTAA+TGG + chr1.4:464672-464691 None:intergenic 20.0%
!!! TATCGATATTCTAAAAAATG+TGG + chr1.4:464479-464498 None:intergenic 20.0%
!!! TGTCTTTTTCTAATAAAGTA+AGG - chr1.4:464319-464338 MS.gene88987:intron 20.0%
!!! TTTTGTGTGTATATTATATC+AGG - chr1.4:465394-465413 MS.gene88987:CDS 20.0%
! AATTATAGCATATAAACTCG+TGG + chr1.4:464017-464036 None:intergenic 25.0%
! ATACAAAAGAATTCGAAAAG+TGG - chr1.4:465637-465656 MS.gene88987:CDS 25.0%
! ATATAAGCTCACATAATTGT+TGG - chr1.4:464793-464812 MS.gene88987:CDS 25.0%
! CACTAAAATAATAGATGCAA+AGG - chr1.4:464731-464750 MS.gene88987:CDS 25.0%
! GGTAAACAGTAATTTATCTT+TGG + chr1.4:465086-465105 None:intergenic 25.0%
! TCTTTATTACTATTGTGTGT+CGG + chr1.4:465107-465126 None:intergenic 25.0%
! TTATATCAGGTATCCAAATA+AGG - chr1.4:465407-465426 MS.gene88987:CDS 25.0%
! TTATTACAAACTCACACAAA+TGG - chr1.4:464517-464536 MS.gene88987:CDS 25.0%
!! ACTTTTCGAATTCTTTTGTA+TGG + chr1.4:465638-465657 None:intergenic 25.0%
!! TGTTTTATGGTTATTGTTAC+AGG - chr1.4:465172-465191 MS.gene88987:CDS 25.0%
!! TTTTAGTGTTATAAACACCT+TGG + chr1.4:464719-464738 None:intergenic 25.0%
!!! AATTTTACCAGATGGATTTT+TGG + chr1.4:465681-465700 None:intergenic 25.0%
!!! ACTCTTTTTTATTAGATGCT+TGG - chr1.4:465552-465571 MS.gene88987:CDS 25.0%
!!! ATTTTACCAGATGGATTTTT+GGG + chr1.4:465680-465699 None:intergenic 25.0%
!!! CATAATTGTTGGTTCTATTA+TGG - chr1.4:464804-464823 MS.gene88987:CDS 25.0%
!!! CTCTTTTTTATTAGATGCTT+GGG - chr1.4:465553-465572 MS.gene88987:CDS 25.0%
AAACTCACACAAATGGATAT+TGG - chr1.4:464524-464543 MS.gene88987:CDS 30.0%
AAAGAGCTCATTAAGTACAA+GGG - chr1.4:464981-465000 MS.gene88987:CDS 30.0%
AACTCACACAAATGGATATT+GGG - chr1.4:464525-464544 MS.gene88987:CDS 30.0%
AAGAAACGAACCTTTCATAA+TGG + chr1.4:463886-463905 None:intergenic 30.0%
AGACCGTATATATGAAACAT+CGG + chr1.4:463408-463427 None:intergenic 30.0%
ATCCATCTGGTAAAATTCTT+CGG - chr1.4:465684-465703 MS.gene88987:CDS 30.0%
ATTAAAATGCAAGACATGGT+TGG - chr1.4:464833-464852 MS.gene88987:CDS 30.0%
CCTAATGTAAGTATCTTTCA+AGG - chr1.4:463684-463703 MS.gene88987:intron 30.0%
CCTTGAAAGATACTTACATT+AGG + chr1.4:463687-463706 None:intergenic 30.0%
CGTAAATATTGTCAAACTCA+TGG - chr1.4:465134-465153 MS.gene88987:CDS 30.0%
GAATTTAGAGAGAACAACTA+TGG + chr1.4:463469-463488 None:intergenic 30.0%
GCTAATTAAAATGCAAGACA+TGG - chr1.4:464829-464848 MS.gene88987:CDS 30.0%
GTAAGTATCTTTCAAGGTTA+TGG - chr1.4:463690-463709 MS.gene88987:intron 30.0%
GTTCCGATGTTTCATATATA+CGG - chr1.4:463402-463421 MS.gene88987:CDS 30.0%
TATACACACAAAACATTCAG+CGG + chr1.4:465387-465406 None:intergenic 30.0%
TATATTCTTTGGTAGGCTTA+CGG + chr1.4:465263-465282 None:intergenic 30.0%
TCCTTTGAGTAAAGAAGTAA+CGG - chr1.4:463641-463660 MS.gene88987:intron 30.0%
TGAATTGCTAAACAAGTTTC+CGG + chr1.4:464080-464099 None:intergenic 30.0%
TGATTGATAAGAAGGAATAC+TGG + chr1.4:465361-465380 None:intergenic 30.0%
!! AAGTGACACTCATTTTGAAA+CGG + chr1.4:464177-464196 None:intergenic 30.0%
!!! GTTCTCTTGCTTTTGTTTTA+TGG - chr1.4:465159-465178 MS.gene88987:CDS 30.0%
AAACGAACCTTTCATAATGG+TGG + chr1.4:463883-463902 None:intergenic 35.0%
AAAGTTTGAGAGGGTAAAGT+TGG + chr1.4:464268-464287 None:intergenic 35.0%
AACGAACCTTTCATAATGGT+GGG + chr1.4:463882-463901 None:intergenic 35.0%
AATGAGAACATGTGGTCTAT+TGG - chr1.4:464136-464155 MS.gene88987:intron 35.0%
ACTCACACAAATGGATATTG+GGG - chr1.4:464526-464545 MS.gene88987:CDS 35.0%
ATCTGGTAAAATTCTTCGGA+AGG - chr1.4:465688-465707 MS.gene88987:CDS 35.0%
ATGAGAATGATTGGAAGTGA+AGG + chr1.4:463138-463157 None:intergenic 35.0%
CAAGTTGTAATGGCTAGATT+TGG - chr1.4:463342-463361 MS.gene88987:intron 35.0%
CATTAAGTACAAGGGATATC+AGG - chr1.4:464989-465008 MS.gene88987:CDS 35.0%
CTGCAACAAAATCCATAACT+TGG + chr1.4:465494-465513 None:intergenic 35.0%
GAAAGAGCTCATTAAGTACA+AGG - chr1.4:464980-464999 MS.gene88987:CDS 35.0%
GAATTGCTAAACAAGTTTCC+GGG + chr1.4:464079-464098 None:intergenic 35.0%
TAATACCAGCAACTAACCAA+AGG + chr1.4:464772-464791 None:intergenic 35.0%
TAGATGCAAAGGAAAAAGCT+TGG - chr1.4:464742-464761 MS.gene88987:CDS 35.0%
TATCAGGTATCCAAATAAGG+AGG - chr1.4:465410-465429 MS.gene88987:CDS 35.0%
TATGGTTTAACTGAATCCTC+TGG - chr1.4:463708-463727 MS.gene88987:intron 35.0%
TCCGTTACTTCTTTACTCAA+AGG + chr1.4:463645-463664 None:intergenic 35.0%
TCTATACCCAAAAATCCATC+TGG - chr1.4:465671-465690 MS.gene88987:CDS 35.0%
TGTGTGAGAAAAGTTTGAGA+GGG + chr1.4:464277-464296 None:intergenic 35.0%
TTCACTTCCAATCATTCTCA+TGG - chr1.4:463137-463156 MS.gene88987:intron 35.0%
TTGATGAGAATGAGAACATG+TGG - chr1.4:464128-464147 MS.gene88987:intron 35.0%
TTGGCACACAGATTAAGAAA+TGG + chr1.4:464385-464404 None:intergenic 35.0%
TTGTGTGAGAAAAGTTTGAG+AGG + chr1.4:464278-464297 None:intergenic 35.0%
! TGAGTAAAGAAGTAACGGAA+GGG - chr1.4:463646-463665 MS.gene88987:intron 35.0%
! TGATTTGAGTTCGCTTCATA+CGG - chr1.4:463602-463621 MS.gene88987:intron 35.0%
! TTCCGAAGAATTTTACCAGA+TGG + chr1.4:465689-465708 None:intergenic 35.0%
! TTGAGTAAAGAAGTAACGGA+AGG - chr1.4:463645-463664 MS.gene88987:intron 35.0%
!! ATGCTTGGGTGATTTTAGAA+AGG + chr1.4:463113-463132 None:intergenic 35.0%
!! GTGATTTTAGAAAGGAGTTG+TGG + chr1.4:463105-463124 None:intergenic 35.0%
!! GTTTGCTATATTGATAGCGA+TGG - chr1.4:464933-464952 MS.gene88987:CDS 35.0%
!! TTAACTGAATCCTCTGGAAT+TGG - chr1.4:463714-463733 MS.gene88987:intron 35.0%
!! TTCAAAATGAGTGTCGCTTT+AGG + chr1.4:464410-464429 None:intergenic 35.0%
!!! GACACTCATTTTGAAACAGA+GGG - chr1.4:464415-464434 MS.gene88987:intron 35.0%
AATGGCATATGTGGTAAGGA+AGG - chr1.4:465446-465465 MS.gene88987:CDS 40.0%
AATTCACCTACCAACAACTC+TGG - chr1.4:462863-462882 MS.gene88987:intron 40.0%
AGAGAGTCGTAAGTATGGTA+CGG - chr1.4:463758-463777 MS.gene88987:intron 40.0%
AGCGGTCTTGATTGATAAGA+AGG + chr1.4:465369-465388 None:intergenic 40.0%
AGTCATGCACGTCTAATGAA+TGG + chr1.4:464694-464713 None:intergenic 40.0%
AGTGAAAGCAAGAACAGGTT+TGG + chr1.4:463079-463098 None:intergenic 40.0%
ATTTCGCGAGATGTATTGAG+GGG + chr1.4:463036-463055 None:intergenic 40.0%
CATCGCTATGAGATTCTTGT+GGG + chr1.4:463319-463338 None:intergenic 40.0%
GAAGATACTACTCCTTTGCT+TGG + chr1.4:463297-463316 None:intergenic 40.0%
GATTTCGCGAGATGTATTGA+GGG + chr1.4:463037-463056 None:intergenic 40.0%
GGAAAGACAAAACATCCAGA+TGG - chr1.4:463989-464008 MS.gene88987:intron 40.0%
GTTGGTCATATGCTTGACTT+AGG - chr1.4:464444-464463 MS.gene88987:intron 40.0%
TAGTTGAAGTGATCGAAACG+TGG + chr1.4:465057-465076 None:intergenic 40.0%
TATAAACTCGTGGATCCATC+TGG + chr1.4:464007-464026 None:intergenic 40.0%
TGAAACAGAGGGAGTAATGT+TGG - chr1.4:464426-464445 MS.gene88987:intron 40.0%
TTCAGCATAGCAACCAGTAT+CGG + chr1.4:463555-463574 None:intergenic 40.0%
TTCCAATGGCATATGTGGTA+AGG - chr1.4:465442-465461 MS.gene88987:CDS 40.0%
TTCCTTACCACATATGCCAT+TGG + chr1.4:465447-465466 None:intergenic 40.0%
TTGGAAGAGAGTCGTAAGTA+TGG - chr1.4:463753-463772 MS.gene88987:intron 40.0%
! ACTGTTTGAGTTCGAACTTG+TGG - chr1.4:464590-464609 MS.gene88987:CDS 40.0%
! AGAGTTGTTGGTAGGTGAAT+TGG + chr1.4:462864-462883 None:intergenic 40.0%
! AGGTTATTTCAGTAACGAGG+AGG - chr1.4:464863-464882 MS.gene88987:CDS 40.0%
! CGATGGATTCTTATTTGTCG+TGG - chr1.4:464950-464969 MS.gene88987:CDS 40.0%
! CTGTTTGAGTTCGAACTTGT+GGG - chr1.4:464591-464610 MS.gene88987:CDS 40.0%
! TATGGATTTTGTTGCAGAGC+AGG - chr1.4:465497-465516 MS.gene88987:CDS 40.0%
!! AGAGGGTTGGTTAAAAACAG+GGG - chr1.4:464908-464927 MS.gene88987:CDS 40.0%
!! AGCGATGGTTCAAGTTGTAA+TGG - chr1.4:463332-463351 MS.gene88987:intron 40.0%
!! CAGAGGGTTGGTTAAAAACA+GGG - chr1.4:464907-464926 MS.gene88987:CDS 40.0%
!! TGTTGTTGATGATGAGTTGC+TGG + chr1.4:463169-463188 None:intergenic 40.0%
!!! CGACACTCATTTTGAAACAG+AGG - chr1.4:464414-464433 MS.gene88987:intron 40.0%
!!! GACACTCATTTTGAAACGGA+TGG + chr1.4:464173-464192 None:intergenic 40.0%
AACAGGGAAGTAGATGGAGT+TGG + chr1.4:462965-462984 None:intergenic 45.0%
AAGTGGTGGCATCTAAAGAG+TGG + chr1.4:462783-462802 None:intergenic 45.0%
ACAGGGAAGTAGATGGAGTT+GGG + chr1.4:462964-462983 None:intergenic 45.0%
ACATCGACCATTACTCCAGA+GGG - chr1.4:464891-464910 MS.gene88987:CDS 45.0%
ACTTCCTCACTCTCCAACAT+GGG - chr1.4:462904-462923 MS.gene88987:CDS 45.0%
AGAGTGATCGATCCAAGAAG+TGG - chr1.4:462697-462716 MS.gene88987:CDS 45.0%
AGATGTATTGAGGGGATTGG+TGG + chr1.4:463028-463047 None:intergenic 45.0%
AGGTATCCAAATAAGGAGGC+TGG - chr1.4:465414-465433 MS.gene88987:CDS 45.0%
AGTAGAAGCTCCAATTCCAG+AGG + chr1.4:463727-463746 None:intergenic 45.0%
CCACAAGAATCTCATAGCGA+TGG - chr1.4:463317-463336 MS.gene88987:intron 45.0%
CCAGTCCGATTAACTGGTAA+CGG + chr1.4:463840-463859 None:intergenic 45.0%
CCATCGCTATGAGATTCTTG+TGG + chr1.4:463320-463339 None:intergenic 45.0%
CGATTTCGCGAGATGTATTG+AGG + chr1.4:463038-463057 None:intergenic 45.0%
CTCAGCCGTTACCAGTTAAT+CGG - chr1.4:463832-463851 MS.gene88987:intron 45.0%
CTCTACTCATCAGGAACAAC+TGG - chr1.4:463270-463289 MS.gene88987:intron 45.0%
CTTGTCAGGAAGCCAAGTTA+TGG - chr1.4:465479-465498 MS.gene88987:CDS 45.0%
GAGTGATCGATCCAAGAAGT+GGG - chr1.4:462698-462717 MS.gene88987:CDS 45.0%
GGAGTAGTGAAAGCAAGAAC+AGG + chr1.4:463084-463103 None:intergenic 45.0%
GGTATCCAAATAAGGAGGCT+GGG - chr1.4:465415-465434 MS.gene88987:CDS 45.0%
GTGGCATCTAAAGAGTGGTT+TGG + chr1.4:462778-462797 None:intergenic 45.0%
GTGGTGTCCATGAGAATGAT+TGG + chr1.4:463147-463166 None:intergenic 45.0%
TATTGAACACGAGGTAGACC+CGG - chr1.4:464058-464077 MS.gene88987:intron 45.0%
TCAGTCACTCTACTCAACTC+AGG + chr1.4:463219-463238 None:intergenic 45.0%
TCCTGATGAGTAGAGTAGTG+TGG + chr1.4:463265-463284 None:intergenic 45.0%
TGGAAGTGAAGGTGATGCTT+GGG + chr1.4:463127-463146 None:intergenic 45.0%
TGGAGCTTCTACTGAGTCTT+TGG - chr1.4:463734-463753 MS.gene88987:intron 45.0%
TTGGAAGTGAAGGTGATGCT+TGG + chr1.4:463128-463147 None:intergenic 45.0%
! GAAAAAGCTTGGTGAGCCTT+TGG - chr1.4:464753-464772 MS.gene88987:CDS 45.0%
! GCAGAGCAGGTTTGTTTGTT+TGG - chr1.4:465510-465529 MS.gene88987:CDS 45.0%
! GCATCTAAAGAGTGGTTTGG+TGG + chr1.4:462775-462794 None:intergenic 45.0%
! GGCAGGTTATTTCAGTAACG+AGG - chr1.4:464860-464879 MS.gene88987:CDS 45.0%
! GTTGTTGGTAGGTGAATTGG+TGG + chr1.4:462861-462880 None:intergenic 45.0%
! TTTGAGTTCGCTTCATACGG+TGG - chr1.4:463605-463624 MS.gene88987:intron 45.0%
!! AAAGAGTGGTTTGGTGGAAG+TGG + chr1.4:462769-462788 None:intergenic 45.0%
!! AACAAAGCCTCTAGTTCTGC+TGG + chr1.4:465203-465222 None:intergenic 45.0%
!! ATGAGTGTCGCTTTAGGCTT+TGG + chr1.4:464404-464423 None:intergenic 45.0%
!! ATGCAAGACATGGTTGGTGA+TGG - chr1.4:464839-464858 MS.gene88987:CDS 45.0%
!! CCAGAGGGTTGGTTAAAAAC+AGG - chr1.4:464906-464925 MS.gene88987:CDS 45.0%
!! GGTCTTGCAGTGTTTGCTAT+GGG - chr1.4:463423-463442 MS.gene88987:CDS 45.0%
!! GTCTTGCAGTGTTTGCTATG+GGG - chr1.4:463424-463443 MS.gene88987:CDS 45.0%
!! TAACCAACCCTCTGGAGTAA+TGG + chr1.4:464901-464920 None:intergenic 45.0%
!! TGTTGATGATGAGTTGCTGG+TGG + chr1.4:463166-463185 None:intergenic 45.0%
!! TTAATCGGACTGGTGAGCTT+TGG - chr1.4:463847-463866 MS.gene88987:intron 45.0%
!!! CCTGTTTTTAACCAACCCTC+TGG + chr1.4:464909-464928 None:intergenic 45.0%
!! TATAATTAATTTATATTCTT+TGG + chr1.4:465274-465293 None:intergenic 5.0%
!!! ATTAATTTTTTTCAATATAT+AGG - chr1.4:465607-465626 MS.gene88987:CDS 5.0%
!!! ATTTATTTTTTATAATATGA+TGG - chr1.4:465326-465345 MS.gene88987:CDS 5.0%
ACAGCAGCGTCTACAATAGC+AGG + chr1.4:465236-465255 None:intergenic 50.0%
ACCTTTACGGATCCCCATGT+TGG + chr1.4:462920-462939 None:intergenic 50.0%
AGCTCACCAGTCCGATTAAC+TGG + chr1.4:463846-463865 None:intergenic 50.0%
AGGCGTCAATGAAGGCGATA+TGG + chr1.4:462831-462850 None:intergenic 50.0%
AGTAGATGGAGTTGGGAGAG+AGG + chr1.4:462957-462976 None:intergenic 50.0%
AGTCGTAAGTATGGTACGGC+GGG - chr1.4:463762-463781 MS.gene88987:intron 50.0%
ATTGTTAGGCAGAGCACACC+AGG + chr1.4:464559-464578 None:intergenic 50.0%
CACATCGACCATTACTCCAG+AGG - chr1.4:464890-464909 MS.gene88987:CDS 50.0%
CACTTCCTCACTCTCCAACA+TGG - chr1.4:462903-462922 MS.gene88987:CDS 50.0%
CCGTTACCAGTTAATCGGAC+TGG - chr1.4:463837-463856 MS.gene88987:intron 50.0%
CGTGGTCCCACCATTATGAA+AGG - chr1.4:463873-463892 MS.gene88987:intron 50.0%
CTGCTGCAAAACCCACTTCT+TGG + chr1.4:462712-462731 None:intergenic 50.0%
CTTCCTCACTCTCCAACATG+GGG - chr1.4:462905-462924 MS.gene88987:CDS 50.0%
GAGTCGTAAGTATGGTACGG+CGG - chr1.4:463761-463780 MS.gene88987:intron 50.0%
GCAGACAACAGGGAAGTAGA+TGG + chr1.4:462971-462990 None:intergenic 50.0%
GCAGGTTCCAATGGCATATG+TGG - chr1.4:465437-465456 MS.gene88987:CDS 50.0%
GCATATGTGGTAAGGAAGGC+TGG - chr1.4:465450-465469 MS.gene88987:CDS 50.0%
GCCACACTACTCTACTCATC+AGG - chr1.4:463261-463280 MS.gene88987:intron 50.0%
GCGAGATGTATTGAGGGGAT+TGG + chr1.4:463031-463050 None:intergenic 50.0%
GGAACAACTGGACCAAGCAA+AGG - chr1.4:463282-463301 MS.gene88987:intron 50.0%
GGAGGATGACGTCACCTTTA+CGG + chr1.4:462933-462952 None:intergenic 50.0%
GGATATTGGGGATCGAATCC+TGG - chr1.4:464538-464557 MS.gene88987:CDS 50.0%
GTAGTGTGGCTGTATCATCC+TGG + chr1.4:463251-463270 None:intergenic 50.0%
GTCAACAATCATAGCCTCCG+TGG + chr1.4:463802-463821 None:intergenic 50.0%
GTTGGAGAGTGAGGAAGTGA+CGG + chr1.4:462902-462921 None:intergenic 50.0%
TAGACGTGCATGACTCTCCA+AGG - chr1.4:464699-464718 MS.gene88987:CDS 50.0%
TCCAACATGGGGATCCGTAA+AGG - chr1.4:462916-462935 MS.gene88987:CDS 50.0%
TCGCTTCATACGGTGGTTTC+TGG - chr1.4:463612-463631 MS.gene88987:intron 50.0%
TGCTATGGGGCTTCTTGCTA+TGG - chr1.4:463437-463456 MS.gene88987:CDS 50.0%
! AAGGCTGGAAGTAGCTTGTC+AGG - chr1.4:465465-465484 MS.gene88987:CDS 50.0%
! AGCATAGCAACCAGTATCGG+CGG + chr1.4:463552-463571 None:intergenic 50.0%
! CGGTCTTGCAGTGTTTGCTA+TGG - chr1.4:463422-463441 MS.gene88987:CDS 50.0%
! CTTCATACGGTGGTTTCTGG+TGG - chr1.4:463615-463634 MS.gene88987:intron 50.0%
! GCTATGGGGCTTCTTGCTAT+GGG - chr1.4:463438-463457 MS.gene88987:CDS 50.0%
! GTGAGCCTTTGGTTAGTTGC+TGG - chr1.4:464764-464783 MS.gene88987:CDS 50.0%
! TGTATTGAGGGGATTGGTGG+TGG + chr1.4:463025-463044 None:intergenic 50.0%
! TTGGTAGGTGAATTGGTGGC+CGG + chr1.4:462857-462876 None:intergenic 50.0%
!! AAAGCCTCTAGTTCTGCTGG+AGG + chr1.4:465200-465219 None:intergenic 50.0%
!! AAGACATGGTTGGTGATGGC+AGG - chr1.4:464843-464862 MS.gene88987:CDS 50.0%
!! AAGCCTCTAGTTCTGCTGGA+GGG + chr1.4:465199-465218 None:intergenic 50.0%
!! AGTGGTTTGGTGGAAGTGGA+AGG + chr1.4:462765-462784 None:intergenic 50.0%
!! CTCTGGAGTAATGGTCGATG+TGG + chr1.4:464892-464911 None:intergenic 50.0%
!! GTGGTTTGGTGGAAGTGGAA+GGG + chr1.4:462764-462783 None:intergenic 50.0%
!! TGGTTTGGTGGAAGTGGAAG+GGG + chr1.4:462763-462782 None:intergenic 50.0%
ACCTGCCCAGCCTCCTTATT+TGG + chr1.4:465423-465442 None:intergenic 55.0%
AGATGGAGTTGGGAGAGAGG+AGG + chr1.4:462954-462973 None:intergenic 55.0%
AGCACGCCAGAGTTGTTGGT+AGG + chr1.4:462872-462891 None:intergenic 55.0%
AGTGACTGAGCGCTTCAACC+AGG - chr1.4:463230-463249 MS.gene88987:intron 55.0%
CGACCATTACTCCAGAGGGT+TGG - chr1.4:464895-464914 MS.gene88987:CDS 55.0%
CTACAGCACGCCAGAGTTGT+TGG + chr1.4:462876-462895 None:intergenic 55.0%
GATCCCCATGTTGGAGAGTG+AGG + chr1.4:462911-462930 None:intergenic 55.0%
GTCGTAAGTATGGTACGGCG+GGG - chr1.4:463763-463782 MS.gene88987:intron 55.0%
TCCAAATAAGGAGGCTGGGC+AGG - chr1.4:465419-465438 MS.gene88987:CDS 55.0%
TGACGGAGAGGCAGACAACA+GGG + chr1.4:462981-463000 None:intergenic 55.0%
! ACTGGTGAGCTTTGGCTTCG+TGG - chr1.4:463855-463874 MS.gene88987:intron 55.0%
! ATGACGGAGAGGCAGACAAC+AGG + chr1.4:462982-463001 None:intergenic 55.0%
ACCACTTGTGCCGCCGATAC+TGG - chr1.4:463539-463558 MS.gene88987:intron 60.0%
AGCGCGGGAGGAGATGAAAG+TGG + chr1.4:462800-462819 None:intergenic 60.0%
ATCTCCTCCCGCGCTCATCA+CGG - chr1.4:462805-462824 MS.gene88987:intron 60.0%
CGATATGGCCGTGATGAGCG+CGG + chr1.4:462816-462835 None:intergenic 60.0%
CGGCGGCACAAGTGGTAAGA+AGG + chr1.4:463535-463554 None:intergenic 60.0%
GATATGGCCGTGATGAGCGC+GGG + chr1.4:462815-462834 None:intergenic 60.0%
GCCTTCATTGACGCCTCCAC+CGG - chr1.4:462835-462854 MS.gene88987:intron 60.0%
GCGGGAGGAGATGAAAGTGG+TGG + chr1.4:462797-462816 None:intergenic 60.0%
GCTTGTGTCTGCTTCCACGG+AGG - chr1.4:463785-463804 MS.gene88987:intron 60.0%
GGGGCTTGTGTCTGCTTCCA+CGG - chr1.4:463782-463801 MS.gene88987:intron 60.0%
GGTCCCTCCAGCAGAACTAG+AGG - chr1.4:465193-465212 MS.gene88987:CDS 60.0%
TGCCTCTCCGTCATGTCCCT+CGG - chr1.4:462988-463007 MS.gene88987:intron 60.0%
TGGAGTTGGGAGAGAGGAGG+AGG + chr1.4:462951-462970 None:intergenic 60.0%
! GATAGCGCCGAGGGACATGA+CGG + chr1.4:462998-463017 None:intergenic 60.0%
! GGTGGTGATGATAGCGCCGA+GGG + chr1.4:463007-463026 None:intergenic 60.0%
! GTAGGTGAATTGGTGGCCGG+TGG + chr1.4:462854-462873 None:intergenic 60.0%
! TGGTGGTGATGATAGCGCCG+AGG + chr1.4:463008-463027 None:intergenic 60.0%
!! ACCAGTATCGGCGGCACAAG+TGG + chr1.4:463543-463562 None:intergenic 60.0%
GGAGGCTGGGCAGGTTCCAA+TGG - chr1.4:465428-465447 MS.gene88987:CDS 65.0%
! GGTGAATTGGTGGCCGGTGG+AGG + chr1.4:462851-462870 None:intergenic 65.0%
!! GCCGGTGGAGGCGTCAATGA+AGG + chr1.4:462839-462858 None:intergenic 65.0%
ATGGCCGTGATGAGCGCGGG+AGG + chr1.4:462812-462831 None:intergenic 70.0%
! CGCCGAGGGACATGACGGAG+AGG + chr1.4:462993-463012 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 462664 465742 462664 ID=MS.gene88987
chr1.4 mRNA 462664 465742 462664 ID=MS.gene88987.t1;Parent=MS.gene88987
chr1.4 exon 464512 465742 464512 ID=MS.gene88987.t1.exon1;Parent=MS.gene88987.t1
chr1.4 CDS 464512 465742 464512 ID=cds.MS.gene88987.t1;Parent=MS.gene88987.t1
chr1.4 exon 463396 463541 463396 ID=MS.gene88987.t1.exon2;Parent=MS.gene88987.t1
chr1.4 CDS 463396 463541 463396 ID=cds.MS.gene88987.t1;Parent=MS.gene88987.t1
chr1.4 exon 463145 463212 463145 ID=MS.gene88987.t1.exon3;Parent=MS.gene88987.t1
chr1.4 CDS 463145 463212 463145 ID=cds.MS.gene88987.t1;Parent=MS.gene88987.t1
chr1.4 exon 462888 462990 462888 ID=MS.gene88987.t1.exon4;Parent=MS.gene88987.t1
chr1.4 CDS 462888 462990 462888 ID=cds.MS.gene88987.t1;Parent=MS.gene88987.t1
chr1.4 exon 462664 462777 462664 ID=MS.gene88987.t1.exon5;Parent=MS.gene88987.t1
chr1.4 CDS 462664 462777 462664 ID=cds.MS.gene88987.t1;Parent=MS.gene88987.t1
Gene Sequence

>MS.gene88987

ATGGAGGTAAACGGTGAGAATACGAATAGCAGAAGAGTGATCGATCCAAGAAGTGGGTTTTGCAGCAGCAATTCAATCTTCTACAGCAAGAGAAAACCCCTTCCACTTCCACCAAACCACTCTTTAGATGCCACCACTTTCATCTCCTCCCGCGCTCATCACGGCCATATCGCCTTCATTGACGCCTCCACCGGCCACCAATTCACCTACCAACAACTCTGGCGTGCTGTAGACTCCGTCACTTCCTCACTCTCCAACATGGGGATCCGTAAAGGTGACGTCATCCTCCTCCTCTCTCCCAACTCCATCTACTTCCCTGTTGTCTGCCTCTCCGTCATGTCCCTCGGCGCTATCATCACCACCACCAATCCCCTCAATACATCTCGCGAAATCGCTAAACAGATCGCTGATTCCAAACCTGTTCTTGCTTTCACTACTCCACAACTCCTTTCTAAAATCACCCAAGCATCACCTTCACTTCCAATCATTCTCATGGACACCACCAGCAACTCATCATCAACAACAACACTTGATGAAATGATGAAGAAAGAACCTGAGTTGAGTAGAGTGACTGAGCGCTTCAACCAGGATGATACAGCCACACTACTCTACTCATCAGGAACAACTGGACCAAGCAAAGGAGTAGTATCTTCCCACAAGAATCTCATAGCGATGGTTCAAGTTGTAATGGCTAGATTTGGTAAAGAACAAGAGACGAGAGAAACTTTCATATGCACTGTTCCGATGTTTCATATATACGGTCTTGCAGTGTTTGCTATGGGGCTTCTTGCTATGGGTTCAACCATAGTTGTTCTCTCTAAATTCGAGATGCATGACATGCTTTCATCTATTGAGAAATTCAGAGCAACCTTCTTACCACTTGTGCCGCCGATACTGGTTGCTATGCTGAACAACGCAGATGCGATTAAGAGAAAGTATGATTTGAGTTCGCTTCATACGGTGGTTTCTGGTGGTGCTCCTTTGAGTAAAGAAGTAACGGAAGGGTTTATAGAAAAGTATCCTAATGTAAGTATCTTTCAAGGTTATGGTTTAACTGAATCCTCTGGAATTGGAGCTTCTACTGAGTCTTTGGAAGAGAGTCGTAAGTATGGTACGGCGGGGCTTGTGTCTGCTTCCACGGAGGCTATGATTGTTGACACTGAGACTGCTCAGCCGTTACCAGTTAATCGGACTGGTGAGCTTTGGCTTCGTGGTCCCACCATTATGAAAGGTTCGTTTCTTTTCAAATGTCGTCGCTTTGTTTCTAATTAATTGATCTTTTGATTCTGAAGATCGCTCTTTTAATAAACAATTCAGATTAGATTGGAAAGACAAAACATCCAGATGGATCCACGAGTTTATATGCTATAATTTCCTTTTTTAATTAATTAAAATATTGAACACGAGGTAGACCCGGAAACTTGTTTAGCAATTCAGAGTTTATATATTTGTATTTGTAGTTGATTGATGAGAATGAGAACATGTGGTCTATTGGAATTTAATACTCCATCCGTTTCAAAATGAGTGTCACTTTAAGCAAAAAAATTTGTTTCAAAATGAATGTCGCTTTACATTTCCAATACAACTTTAATTTTTTTCTTCCAACTTTACCCTCTCAAACTTTTCTCACACAATTTTCAATGCAACTTTAAGTTTGTCTTTTTCTAATAAAGTAAGGACAGTTTAGTAAAAGCGTTATATCCAATGATTATTTCATTCCATTTCTTAATCTGTGTGCCAAAGCCTAAAGCGACACTCATTTTGAAACAGAGGGAGTAATGTTGGTCATATGCTTGACTTAGGCTATATATCCCACATTTTTTAGAATATCGATAGACAAAATGACAAAACATTTATTACAAACTCACACAAATGGATATTGGGGATCGAATCCTGGTGTGCTCTGCCTAACAATTTTGACATTTTACTGTTTGAGTTCGAACTTGTGGGCTTTCGCTTTTCTAATTTAATAAATTTAATTTTTATTTAATAAAAAAAGAAGTAACCATTAGACATAAAAAAAGTAACCATTCATTAGACGTGCATGACTCTCCAAGGTGTTTATAACACTAAAATAATAGATGCAAAGGAAAAAGCTTGGTGAGCCTTTGGTTAGTTGCTGGTATTAGATATAAGCTCACATAATTGTTGGTTCTATTATGGATGCTAATTAAAATGCAAGACATGGTTGGTGATGGCAGGTTATTTCAGTAACGAGGAGGCAACCACATCGACCATTACTCCAGAGGGTTGGTTAAAAACAGGGGATGTTTGCTATATTGATAGCGATGGATTCTTATTTGTCGTGGATCGCTTGAAAGAGCTCATTAAGTACAAGGGATATCAGGTTATTATTAACAATTCTATCTAATCTAATGCAGTATTCACAACCACGTTTCGATCACTTCAACTAAATAACCCAAAGATAAATTACTGTTTACCGACACACAATAGTAATAAAGATTATAAACGTAAATATTGTCAAACTCATGGAAGTTCTCTTGCTTTTGTTTTATGGTTATTGTTACAGGTCCCTCCAGCAGAACTAGAGGCTTTGTTGCTGACTCATCCTGCTATTGTAGACGCTGCTGTCATACCGTAAGCCTACCAAAGAATATAAATTAATTATAGTATCTTAATTTTAGTTTTTTTGTCTGCTTTGATTTATTTTTTATAATATGATGGTGACGAAAACCAGTATTCCTTCTTATCAATCAAGACCGCTGAATGTTTTGTGTGTATATTATATCAGGTATCCAAATAAGGAGGCTGGGCAGGTTCCAATGGCATATGTGGTAAGGAAGGCTGGAAGTAGCTTGTCAGGAAGCCAAGTTATGGATTTTGTTGCAGAGCAGGTTTGTTTGTTTGGAACACTTCTCAACAATGACACTCTTTTTTATTAGATGCTTGGGTTTTGATTTCAACTATGAGATTGAAAACTAAATTAATTTTTTTCAATATATAGGTGGCTCCATACAAAAGAATTCGAAAAGTGGCATTTATATCTTCTATACCCAAAAATCCATCTGGTAAAATTCTTCGGAAGGATCTTATCAAGCTAGCTACGTCTAAACTTTGA
Protein sequence

>MS.gene88987.t1

MEVNGENTNSRRVIDPRSGFCSSNSIFYSKRKPLPLPPNHSLDATTFISSRAHHGHIAFIDASTGHQFTYQQLWRAVDSVTSSLSNMGIRKGDVILLLSPNSIYFPVVCLSVMSLGAIITTTNPLNTSREIAKQIADSKPVLAFTTPQLLSKITQASPSLPIILMDTTSNSSSTTTLDEMMKKEPELSRVTERFNQDDTATLLYSSGTTGPSKGVVSSHKNLIAMVQVVMARFGKEQETRETFICTVPMFHIYGLAVFAMGLLAMGSTIVVLSKFEMHDMLSSIEKFRATFLPLVPPILVAMLNNADAIKRKYDLSSLHTVVSGGAPLSKEVTEGFIEKYPNVSIFQGYGLTESSGIGASTESLEESRKYGTAGLVSASTEAMIVDTETAQPLPVNRTGELWLRGPTIMKGYFSNEEATTSTITPEGWLKTGDVCYIDSDGFLFVVDRLKELIKYKGYQVPPAELEALLLTHPAIVDAAVIPYPNKEAGQVPMAYVVRKAGSSLSGSQVMDFVAEQVAPYKRIRKVAFISSIPKNPSGKILRKDLIKLATSKL