AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028035

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028035.t1 MTR_5g027480 92.613 555 41 0 1 555 1 555 0.0 1048
MS.gene028035.t1 MTR_5g027500 78.873 568 107 2 1 555 1 568 0.0 876
MS.gene028035.t1 MTR_4g077900 79.707 478 95 1 80 555 1 478 0.0 775
MS.gene028035.t1 MTR_1g026310 44.224 554 296 5 11 555 11 560 1.49e-155 456
MS.gene028035.t1 MTR_8g039720 43.915 567 294 9 5 555 1 559 6.69e-151 444
MS.gene028035.t1 MTR_1g090737 44.118 544 280 10 18 547 25 558 6.57e-141 419
MS.gene028035.t1 MTR_4g057730 42.301 539 294 9 18 547 4 534 3.26e-139 414
MS.gene028035.t1 MTR_2g095510 40.520 538 305 7 25 555 10 539 5.06e-136 406
MS.gene028035.t1 MTR_3g088870 41.331 496 273 7 58 547 67 550 6.52e-127 383
MS.gene028035.t1 MTR_1g063120 38.798 549 305 10 20 547 6 544 2.49e-126 381
MS.gene028035.t1 MTR_1g063110 38.532 545 312 8 18 547 4 540 3.78e-124 376
MS.gene028035.t1 MTR_3g088880 40.443 497 281 6 54 547 64 548 8.14e-124 375
MS.gene028035.t1 MTR_4g057730 41.983 474 258 9 18 482 4 469 3.04e-114 347
MS.gene028035.t1 MTR_1g073180 39.341 516 288 9 42 549 30 528 9.63e-109 335
MS.gene028035.t1 MTR_4g128337 39.400 500 285 10 58 547 49 540 5.28e-107 332
MS.gene028035.t1 MTR_4g057730 41.410 454 249 9 18 462 4 449 1.00e-105 325
MS.gene028035.t1 MTR_2g105570 38.910 532 303 11 30 548 20 542 3.91e-105 327
MS.gene028035.t1 MTR_2g095510 39.326 445 255 7 25 462 10 446 2.50e-101 314
MS.gene028035.t1 MTR_4g005750 39.811 530 295 13 30 548 19 535 5.48e-100 313
MS.gene028035.t1 MTR_5g007640 38.067 507 295 7 58 553 87 585 3.62e-99 312
MS.gene028035.t1 MTR_3g460770 33.945 545 328 8 26 555 14 541 2.68e-98 309
MS.gene028035.t1 MTR_1g073180 37.918 538 298 13 9 530 14 531 1.18e-97 306
MS.gene028035.t1 MTR_1g026470 38.481 395 230 5 11 396 11 401 1.17e-78 253
MS.gene028035.t1 MTR_1g026430 38.481 395 230 5 11 396 11 401 1.58e-78 253
MS.gene028035.t1 MTR_3g035130 30.041 486 320 9 68 545 32 505 3.23e-52 186
MS.gene028035.t1 MTR_5g076240 28.244 524 317 17 66 549 38 542 1.90e-43 163
MS.gene028035.t1 MTR_3g035130 29.773 440 289 9 68 499 32 459 1.83e-41 155
MS.gene028035.t1 MTR_1569s0010 25.573 524 332 11 68 549 22 529 1.32e-40 154
MS.gene028035.t1 MTR_4g011790 25.887 479 310 11 109 554 132 598 9.05e-38 147
MS.gene028035.t1 MTR_0803s0020 27.525 505 300 13 70 541 263 734 7.15e-37 147
MS.gene028035.t1 MTR_4g011790 25.615 488 309 13 109 554 132 607 5.49e-36 142
MS.gene028035.t1 MTR_2g030240 25.878 541 321 13 71 555 31 547 3.54e-35 139
MS.gene028035.t1 MTR_4g011790 25.696 467 302 11 109 542 132 586 1.22e-34 138
MS.gene028035.t1 MTR_5g076220 27.807 374 232 10 196 548 186 542 1.55e-34 137
MS.gene028035.t1 MTR_5g008800 28.008 507 330 14 67 549 39 534 3.20e-34 136
MS.gene028035.t1 MTR_3g064420 26.168 535 322 19 64 548 36 547 1.19e-33 135
MS.gene028035.t1 MTR_2g044180 30.055 366 227 12 201 548 107 461 5.88e-33 132
MS.gene028035.t1 MTR_2g044180 27.129 505 320 15 81 548 59 552 7.75e-33 132
MS.gene028035.t1 MTR_4g013210 27.475 404 250 12 182 554 54 445 1.87e-32 130
MS.gene028035.t1 MTR_5g008820 26.796 515 318 16 69 548 41 531 2.44e-32 131
MS.gene028035.t1 MTR_0161s0050 27.128 376 232 12 196 548 188 544 9.96e-32 129
MS.gene028035.t1 MTR_8g074040 27.505 509 304 20 80 548 94 577 1.86e-31 129
MS.gene028035.t1 MTR_4g121600 25.382 524 329 16 69 548 47 552 2.10e-31 128
MS.gene028035.t1 MTR_5g076200 27.273 374 234 11 196 548 186 542 8.03e-31 127
MS.gene028035.t1 MTR_8g074040 28.831 385 231 14 185 549 99 460 3.34e-30 124
MS.gene028035.t1 MTR_8g074120 29.223 373 223 12 198 549 137 489 4.97e-29 121
MS.gene028035.t1 MTR_2g030240 27.387 398 234 10 197 555 72 453 9.81e-29 119
MS.gene028035.t1 MTR_1g026350 41.304 138 81 0 11 148 11 148 3.22e-27 108
MS.gene028035.t1 MTR_4g013210 26.234 385 238 11 182 554 54 404 5.47e-27 113
MS.gene028035.t1 MTR_4g013210 25.189 397 227 9 182 554 206 556 1.79e-26 114
MS.gene028035.t1 MTR_5g023730 26.306 517 327 18 69 553 48 542 4.30e-26 112
MS.gene028035.t1 MTR_7g084850 24.194 558 359 16 43 555 186 724 2.37e-25 111
MS.gene028035.t1 MTR_4g013210 27.151 372 230 11 182 523 206 566 3.62e-25 110
MS.gene028035.t1 MTR_4g057755 55.556 72 32 0 411 482 46 117 4.93e-22 92.0
MS.gene028035.t1 MTR_1g104810 25.048 523 308 16 23 472 57 568 4.58e-21 97.8
MS.gene028035.t1 MTR_3g087980 24.897 486 275 16 68 475 106 579 4.15e-20 94.7
MS.gene028035.t1 MTR_1g030290 26.989 352 173 15 198 480 250 586 9.21e-20 93.6
MS.gene028035.t1 MTR_1g104810 27.778 324 177 10 195 472 189 501 9.54e-20 93.6
MS.gene028035.t1 MTR_7g109830 24.197 529 305 19 37 484 36 549 2.18e-18 89.4
MS.gene028035.t1 MTR_1054s0020 24.930 357 243 11 200 549 432 770 4.53e-18 88.6
MS.gene028035.t1 MTR_8g099420 25.879 398 217 14 140 484 180 552 5.71e-18 87.8
MS.gene028035.t1 MTR_2g098490 26.566 399 231 18 198 544 647 1035 6.35e-16 82.0
MS.gene028035.t1 MTR_4g066130 25.750 400 234 19 197 544 650 1038 3.70e-15 79.7
MS.gene028035.t1 MTR_8g083170 30.180 222 133 7 324 532 459 671 6.15e-15 78.6
MS.gene028035.t1 MTR_3g088890 48.438 64 33 0 457 520 10 73 2.50e-14 68.9
MS.gene028035.t1 MTR_3g084470 30.769 221 122 7 296 495 418 628 3.10e-13 73.2
MS.gene028035.t1 MTR_3g084450 30.808 198 114 7 315 495 233 424 9.35e-13 71.2
MS.gene028035.t1 MTR_3g084450 30.808 198 114 7 315 495 441 632 2.37e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028035.t1 AT4G19010 58.318 547 223 3 10 551 12 558 0.0 644
MS.gene028035.t1 AT1G20510 44.403 536 285 5 18 547 8 536 8.67e-160 467
MS.gene028035.t1 AT1G20480 42.495 553 299 6 14 555 21 565 3.89e-149 440
MS.gene028035.t1 AT1G20500 42.162 555 301 8 11 555 6 550 1.42e-146 433
MS.gene028035.t1 AT4G05160 40.516 543 307 6 23 555 8 544 5.08e-139 414
MS.gene028035.t1 AT5G38120 40.868 553 303 9 6 547 3 542 1.16e-134 403
MS.gene028035.t1 AT5G63380 39.927 551 307 7 12 547 9 550 2.43e-134 402
MS.gene028035.t1 AT1G20510 43.312 471 254 5 18 482 8 471 4.19e-133 396
MS.gene028035.t1 AT1G20510 42.350 451 247 5 18 462 8 451 2.67e-122 368
MS.gene028035.t1 AT5G38120 39.810 525 292 9 6 519 3 514 7.38e-121 366
MS.gene028035.t1 AT3G21240 40.187 535 301 10 30 555 30 554 2.78e-111 343
MS.gene028035.t1 AT1G65060 41.129 496 283 7 58 548 66 557 3.02e-110 340
MS.gene028035.t1 AT5G38120 39.549 488 271 9 6 482 3 477 1.89e-109 335
MS.gene028035.t1 AT5G38120 39.549 488 271 9 6 482 3 477 1.89e-109 335
MS.gene028035.t1 AT1G51680 42.240 509 280 8 58 555 56 561 1.21e-108 336
MS.gene028035.t1 AT3G21230 38.899 527 306 9 40 553 43 566 2.54e-103 323
MS.gene028035.t1 AT1G51680 41.860 473 261 8 58 519 56 525 2.10e-96 304
MS.gene028035.t1 AT1G62940 37.624 505 290 8 57 549 43 534 2.38e-96 303
MS.gene028035.t1 AT3G21230 38.540 493 287 9 40 519 43 532 1.10e-91 292
MS.gene028035.t1 AT1G20490 37.963 432 248 8 11 431 6 428 4.54e-89 282
MS.gene028035.t1 AT1G20490 37.963 432 248 8 11 431 6 428 4.54e-89 282
MS.gene028035.t1 AT3G21240 38.793 464 265 10 30 484 30 483 4.77e-86 275
MS.gene028035.t1 AT3G21240 38.793 464 265 10 30 484 30 483 4.77e-86 275
MS.gene028035.t1 AT1G51680 40.411 438 247 8 58 484 56 490 4.96e-83 267
MS.gene028035.t1 AT3G21230 37.719 456 268 9 40 482 43 495 9.37e-80 259
MS.gene028035.t1 AT1G65060 39.078 412 242 7 58 464 66 473 2.52e-78 255
MS.gene028035.t1 AT3G48990 29.569 487 323 12 67 545 30 504 2.62e-49 178
MS.gene028035.t1 AT3G21230 33.075 387 243 9 40 413 43 426 3.33e-44 163
MS.gene028035.t1 AT1G77240 32.637 383 214 15 195 552 182 545 1.77e-39 151
MS.gene028035.t1 AT1G65890 26.128 532 326 18 66 549 38 550 2.52e-37 146
MS.gene028035.t1 AT1G21530 26.460 548 341 21 41 549 19 543 3.22e-37 145
MS.gene028035.t1 AT5G16370 26.839 503 319 14 80 549 53 539 4.72e-37 145
MS.gene028035.t1 AT1G30520 26.679 521 319 15 77 549 42 547 5.62e-37 144
MS.gene028035.t1 AT1G30520 26.679 521 319 15 77 549 42 547 5.62e-37 144
MS.gene028035.t1 AT1G21530 26.374 546 342 21 41 549 19 541 6.19e-36 141
MS.gene028035.t1 AT1G65880 26.996 526 330 17 66 549 38 551 8.62e-36 141
MS.gene028035.t1 AT1G21540 30.623 369 224 12 204 552 194 550 1.67e-35 140
MS.gene028035.t1 AT1G66120 26.857 525 324 17 66 549 38 543 2.91e-33 134
MS.gene028035.t1 AT1G75960 29.650 371 228 13 196 548 183 538 5.33e-33 133
MS.gene028035.t1 AT5G16340 26.680 506 316 18 80 549 53 539 3.33e-32 130
MS.gene028035.t1 AT3G16910 28.238 386 250 10 181 549 181 556 4.24e-31 127
MS.gene028035.t1 AT1G20560 27.273 506 313 17 80 549 54 540 6.43e-31 127
MS.gene028035.t1 AT3G16170 24.846 487 299 16 109 554 147 607 4.94e-30 125
MS.gene028035.t1 AT1G20560 29.891 368 227 12 198 549 110 462 2.40e-29 121
MS.gene028035.t1 AT1G30520 25.909 440 274 13 77 482 42 463 4.85e-29 120
MS.gene028035.t1 AT1G30520 25.909 440 274 13 77 482 42 463 4.85e-29 120
MS.gene028035.t1 AT5G36880 25.047 531 348 14 67 555 197 719 2.04e-28 120
MS.gene028035.t1 AT5G36880 25.047 531 348 14 67 555 147 669 2.31e-28 120
MS.gene028035.t1 AT5G36880 25.047 531 348 14 67 555 147 669 2.31e-28 120
MS.gene028035.t1 AT5G36880 25.047 531 348 14 67 555 147 669 2.31e-28 120
MS.gene028035.t1 AT5G36880 25.047 531 348 14 67 555 147 669 2.31e-28 120
MS.gene028035.t1 AT1G76290 26.747 501 316 16 80 549 52 532 2.72e-28 119
MS.gene028035.t1 AT2G17650 28.457 376 227 14 196 549 240 595 7.16e-28 118
MS.gene028035.t1 AT1G68270 23.864 528 311 18 66 548 38 519 4.99e-26 112
MS.gene028035.t1 AT5G27600 26.616 526 292 17 23 470 60 569 1.24e-24 108
MS.gene028035.t1 AT5G36880 25.163 461 298 14 67 488 147 599 3.33e-22 101
MS.gene028035.t1 AT1G77590 23.975 488 288 15 68 482 107 584 1.37e-18 90.1
MS.gene028035.t1 AT3G05970 25.047 535 283 18 26 470 63 569 1.86e-18 89.7
MS.gene028035.t1 AT1G77590 26.225 347 186 12 198 482 100 438 8.53e-18 87.0
MS.gene028035.t1 AT3G23790 29.070 172 104 5 335 493 465 631 4.55e-11 66.2
MS.gene028035.t1 AT2G47240 31.548 168 109 5 320 484 326 490 6.10e-11 65.5
MS.gene028035.t1 AT2G47240 31.548 168 109 5 320 484 385 549 6.90e-11 65.5
MS.gene028035.t1 AT2G47240 31.548 168 109 5 320 484 385 549 6.90e-11 65.5
MS.gene028035.t1 AT2G47240 31.548 168 109 5 320 484 385 549 6.90e-11 65.5

Find 122 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTATTGATTCCTCTTC+TGG 0.119793 5.3:-17092754 MS.gene028035:CDS
TTGAAATTGATGAGACTATT+AGG 0.168502 5.3:-17092093 MS.gene028035:CDS
GGCTTCTGGTCTTCATAAAA+TGG 0.204210 5.3:-17092686 MS.gene028035:CDS
ATGGGATAGTAAATTGAATT+TGG 0.204514 5.3:+17092628 None:intergenic
TATCAAATACAAGGGCTTTC+AGG 0.229499 5.3:-17091189 MS.gene028035:intron
TATTGCCATGGTTGAGCTTT+TGG 0.243376 5.3:-17092260 MS.gene028035:CDS
TACTATCCCATTGTACTATT+AGG 0.254485 5.3:-17092616 MS.gene028035:CDS
CTTAGGATACTTGAATAATA+AGG 0.261353 5.3:-17091318 MS.gene028035:intron
CCCTTATTGATTCCTCTTCT+GGG 0.272214 5.3:-17092753 MS.gene028035:CDS
TTGCCGATGTTCCATTTATA+TGG 0.273927 5.3:-17092178 MS.gene028035:CDS
ATAGTGGATGTCGCCGTTAC+AGG 0.280831 5.3:-17091003 MS.gene028035:CDS
AGAGAGATAAACCATATAAA+TGG 0.302429 5.3:+17092167 None:intergenic
ATATCATCACTCACCCTTCT+TGG 0.305510 5.3:-17092891 MS.gene028035:CDS
ACCTGTATAAAATCAACATT+AGG 0.307157 5.3:+17091899 None:intergenic
GCGAAGGCAAAGGGTGTAAA+TGG 0.324668 5.3:-17092007 MS.gene028035:CDS
TGCTACGGGATTATTGTCAT+TGG 0.327188 5.3:-17092143 MS.gene028035:CDS
TGGAGGCAAAAGTGGTATAT+TGG 0.338544 5.3:-17091660 MS.gene028035:CDS
TGAAATTGATGAGACTATTA+GGG 0.342348 5.3:-17092092 MS.gene028035:CDS
TTAGTCAAATCAATGGCTTC+TGG 0.343881 5.3:-17092700 MS.gene028035:CDS
AGCAGTTTCTGAGTTGCTTT+CGG 0.356180 5.3:+17090597 None:intergenic
ACGGCGACATCCACTATTTC+AGG 0.369334 5.3:+17091009 None:intergenic
ATGACTGAGTCGGGTGCATT+AGG 0.374967 5.3:-17091748 MS.gene028035:CDS
CTCCCCAGCTTCTTCATCCT+TGG 0.375919 5.3:+17090885 None:intergenic
CAGGCAGAAGTGGTGAGCTT+TGG 0.376467 5.3:-17091615 MS.gene028035:CDS
TACATGCCTTAGAGTTTAGA+AGG 0.376709 5.3:+17090572 None:intergenic
AACGATACAACGTCGAGAAA+AGG 0.386540 5.3:+17092808 None:intergenic
TGCTTGCCTTCTAAACTCTA+AGG 0.391570 5.3:-17090578 None:intergenic
TCCCAGAAGAGGAATCAATA+AGG 0.395051 5.3:+17092752 None:intergenic
ACTTTGCGAACCTTCTTGAA+TGG 0.396762 5.3:+17090665 None:intergenic
GGAGATTCCAGTGGGATTTG+TGG 0.412173 5.3:-17090866 MS.gene028035:CDS
GTGTATACTATTGTAAATTC+CGG 0.412626 5.3:+17092855 None:intergenic
GCTACGGGATTATTGTCATT+GGG 0.419178 5.3:-17092142 MS.gene028035:CDS
TGGTTTATCTCTCTTTGCTA+CGG 0.427906 5.3:-17092158 MS.gene028035:CDS
AAACAAAGTTACCAGAAATC+AGG 0.431755 5.3:+17092386 None:intergenic
TAACAACTTTGAGTAAGAAA+TGG 0.436153 5.3:+17092726 None:intergenic
AAAGATGGTTGGCTACATAC+TGG 0.442873 5.3:-17091272 MS.gene028035:CDS
GCTGTCAATGCCGACAATAT+CGG 0.443469 5.3:+17092028 None:intergenic
GGTTTATCTCTCTTTGCTAC+GGG 0.445435 5.3:-17092157 MS.gene028035:CDS
GAAGCCATTGATTTGACTAA+AGG 0.445775 5.3:+17092703 None:intergenic
CAAAGCTCACCACTTCTGCC+TGG 0.447396 5.3:+17091616 None:intergenic
TATCGATTTCGTTGCTAAGC+AGG 0.452311 5.3:-17090806 MS.gene028035:intron
TCTTGTTCCACCGATATTGT+CGG 0.455719 5.3:-17092038 MS.gene028035:CDS
GTTACCTTTAGTCAAATCAA+TGG 0.458910 5.3:-17092707 MS.gene028035:CDS
AAAGCTCACCACTTCTGCCT+GGG 0.459009 5.3:+17091617 None:intergenic
ACGAAATCGATAACATCCTT+TGG 0.459388 5.3:+17090817 None:intergenic
GGATTTGTGGTCAAGAAGGT+TGG 0.460966 5.3:-17090853 MS.gene028035:CDS
ACGATACAACGTCGAGAAAA+GGG 0.466770 5.3:+17092809 None:intergenic
TTTCGCAACTATTCTTCACT+AGG 0.467126 5.3:-17091700 MS.gene028035:CDS
CCTGTATAAAATCAACATTA+GGG 0.467794 5.3:+17091900 None:intergenic
TTCTGTGCCAGAAAATGAAA+AGG 0.468038 5.3:-17092443 MS.gene028035:CDS
AAGATTCCGAGGTCTGTGAC+TGG 0.468807 5.3:-17090629 MS.gene028035:CDS
GCTTCTGGTCTTCATAAAAT+GGG 0.474868 5.3:-17092685 MS.gene028035:CDS
AATCAACATTAGGGAGAGCT+TGG 0.474883 5.3:+17091909 None:intergenic
ATAACACCTAATAGTACAAT+GGG 0.478275 5.3:+17092610 None:intergenic
GCTAAGAAATTAGAACCATT+AGG 0.483798 5.3:-17092478 MS.gene028035:CDS
AAAGTGGTATATTGGAATAG+TGG 0.491834 5.3:-17091652 MS.gene028035:CDS
ATTTCATCAACACTACTAAG+AGG 0.496577 5.3:+17092556 None:intergenic
CACAGACCTCGGAATCTTGT+CGG 0.496944 5.3:+17090634 None:intergenic
GAAAGAATTTATCAAATACA+AGG 0.497893 5.3:-17091198 MS.gene028035:CDS
GGGTCGGTCGGAAGGCTCCT+AGG 0.498932 5.3:+17092829 None:intergenic
TGTTGATGAAATTCGTAAGC+AGG 0.500410 5.3:-17092545 MS.gene028035:CDS
GTTCTTCAATCGTTGTAATG+AGG 0.501221 5.3:-17092120 MS.gene028035:CDS
CCCAGAAGAGGAATCAATAA+GGG 0.505561 5.3:+17092753 None:intergenic
GAAATAATAGGAATGCCTAA+TGG 0.507899 5.3:+17092463 None:intergenic
TAAGTTGCAGAGTTTGAGAC+AGG 0.511530 5.3:-17091981 MS.gene028035:CDS
AGTGGGATTTGTGGTCAAGA+AGG 0.512122 5.3:-17090857 MS.gene028035:CDS
GTTTATGGAATGACTGAGTC+GGG 0.516171 5.3:-17091757 MS.gene028035:CDS
GGTTTATGGAATGACTGAGT+CGG 0.521041 5.3:-17091758 MS.gene028035:CDS
AGTGGTGAGCTTTGGTTGAG+AGG 0.521413 5.3:-17091607 MS.gene028035:CDS
AGTGCCAAGGATGAAGAAGC+TGG 0.523191 5.3:-17090889 MS.gene028035:CDS
ATGTCGACAATTGATAAAGA+TGG 0.526979 5.3:-17091287 MS.gene028035:CDS
AACACGGCTTCTAAATCAGC+AGG 0.533730 5.3:+17091042 None:intergenic
TCCAAACATGGAGGCAAAAG+TGG 0.536470 5.3:-17091668 MS.gene028035:CDS
ACAATAGTATACACTCTCCT+AGG 0.538812 5.3:-17092846 MS.gene028035:CDS
TCATAGAAATCTTATTGCCA+TGG 0.541662 5.3:-17092272 MS.gene028035:CDS
TTCGCAACTATTCTTCACTA+GGG 0.541708 5.3:-17091699 MS.gene028035:CDS
GCAGGTTGCTCCATTCAAGA+AGG 0.547112 5.3:-17090675 MS.gene028035:intron
CGAGAAAAGGGTCGGTCGGA+AGG 0.547817 5.3:+17092821 None:intergenic
TAGTGGATGTCGCCGTTACA+GGG 0.551826 5.3:-17091002 MS.gene028035:intron
GTGCCAAGGATGAAGAAGCT+GGG 0.553492 5.3:-17090888 MS.gene028035:CDS
TTCTTGACCACAAATCCCAC+TGG 0.554243 5.3:+17090859 None:intergenic
CATAACACCTAATAGTACAA+TGG 0.557647 5.3:+17092609 None:intergenic
GCATTGACAGCGAAGGCAAA+GGG 0.561700 5.3:-17092016 MS.gene028035:CDS
ACACTACTAAGAGGATTCAA+TGG 0.565007 5.3:+17092565 None:intergenic
GGCATTGACAGCGAAGGCAA+AGG 0.570107 5.3:-17092017 MS.gene028035:CDS
ATTTGATAAATTCTTTCAAG+CGG 0.572308 5.3:+17091204 None:intergenic
AGAGGTCCTTCAATTATGAA+AGG 0.572900 5.3:-17091589 MS.gene028035:intron
AAGCTCACCACTTCTGCCTG+GGG 0.573330 5.3:+17091618 None:intergenic
CGACAATTGATAAAGATGGT+TGG 0.573781 5.3:-17091283 MS.gene028035:CDS
AGATTCCGAGGTCTGTGACT+GGG 0.575854 5.3:-17090628 MS.gene028035:CDS
ATCTTCCCAGTCACAGACCT+CGG 0.576821 5.3:+17090623 None:intergenic
AAAGAATTTATCAAATACAA+GGG 0.580755 5.3:-17091197 MS.gene028035:CDS
CATAAAATGGGAGTTTCACA+AGG 0.583635 5.3:-17092673 MS.gene028035:CDS
CTATTAGGTGTTATGTACCT+AGG 0.587403 5.3:-17092601 MS.gene028035:CDS
GGTACTACTGGTGTGAGCAA+AGG 0.591807 5.3:-17092307 MS.gene028035:CDS
ATTCAAGAAGGTTCGCAAAG+TGG 0.594840 5.3:-17090663 MS.gene028035:CDS
AAACCATATAAATGGAACAT+CGG 0.598356 5.3:+17092175 None:intergenic
AGAAATGGAAGTCCCAGAAG+AGG 0.600253 5.3:+17092741 None:intergenic
TTCAGGATGCAAAATTAACA+CGG 0.600524 5.3:+17091026 None:intergenic
AGGGCTAAAACCCCATCATG+AGG 0.601023 5.3:+17092772 None:intergenic
TTCATATACTTACCCTGTAA+CGG 0.602675 5.3:+17090990 None:intergenic
TTGCAGAGTTTGAGACAGGT+CGG 0.607467 5.3:-17091977 MS.gene028035:CDS
AGTTTCTGAGTTGCTTTCGG+AGG 0.613327 5.3:+17090600 None:intergenic
AGGGTTGTTAGCTCCAAACA+TGG 0.614345 5.3:-17091680 MS.gene028035:CDS
TGGAAGTGTACTATCTCCAA+AGG 0.615514 5.3:-17090833 MS.gene028035:CDS
TGCCAAGGATGAAGAAGCTG+GGG 0.626607 5.3:-17090887 MS.gene028035:CDS
TCGGGTGCATTAGGAGCATG+CGG 0.630001 5.3:-17091739 MS.gene028035:CDS
GTCAATGCCGACAATATCGG+TGG 0.636107 5.3:+17092031 None:intergenic
AGCTCACCACTTCTGCCTGG+GGG 0.642781 5.3:+17091619 None:intergenic
GGGCTAAAACCCCATCATGA+GGG 0.653343 5.3:+17092773 None:intergenic
CGTAAGCAGGTTAATGACTG+TGG 0.656452 5.3:-17092532 MS.gene028035:CDS
ACGTCGAGAAAAGGGTCGGT+CGG 0.657613 5.3:+17092817 None:intergenic
ATTGTCGGCATTGACAGCGA+AGG 0.665271 5.3:-17092023 MS.gene028035:CDS
CCTCGCCAAAAGCTCAACCA+TGG 0.675519 5.3:+17092255 None:intergenic
GAAGCTGGGGAGATTCCAGT+GGG 0.676967 5.3:-17090874 MS.gene028035:CDS
TTGGAAGTAGAAGCAAAACA+AGG 0.677547 5.3:+17092647 None:intergenic
AGAAGCTGGGGAGATTCCAG+TGG 0.677976 5.3:-17090875 MS.gene028035:CDS
TACAACGTCGAGAAAAGGGT+CGG 0.686772 5.3:+17092813 None:intergenic
ACGGCTTCTAAATCAGCAGG+AGG 0.692774 5.3:+17091045 None:intergenic
ATGGTGTAACAACAGCACCT+AGG 0.700872 5.3:+17092584 None:intergenic
TCTTCACCGACAAGATTCCG+AGG 0.707806 5.3:-17090640 MS.gene028035:CDS
GTTGTTAGCTCCAAACATGG+AGG 0.741487 5.3:-17091677 MS.gene028035:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAATTTATCAAATACAA+GGG - chr5.3:17092308-17092327 MS.gene028035:CDS 15.0%
!! GTTTGATTAATAATTTCAAT+TGG + chr5.3:17092153-17092172 None:intergenic 15.0%
!! TTATATTTAAATGTCTAAAG+AGG + chr5.3:17092566-17092585 None:intergenic 15.0%
!!! ATGTAAAATTGTTTCTTTTT+AGG - chr5.3:17091726-17091745 MS.gene028035:CDS 15.0%
!! AAACAACATTATTTGAATGA+TGG + chr5.3:17091680-17091699 None:intergenic 20.0%
!! ATTTGATAAATTCTTTCAAG+CGG + chr5.3:17092304-17092323 None:intergenic 20.0%
!! GAAAGAATTTATCAAATACA+AGG - chr5.3:17092307-17092326 MS.gene028035:CDS 20.0%
!! TAAATACTAAAAGGATAACA+TGG - chr5.3:17091978-17091997 MS.gene028035:CDS 20.0%
!!! AACTTTTTCCTTAATTTCTT+AGG - chr5.3:17092170-17092189 MS.gene028035:CDS 20.0%
!!! AATAAGACTATTTTATTCTG+AGG + chr5.3:17091637-17091656 None:intergenic 20.0%
!!! AATTTTAACCCTTTTTTTGT+TGG - chr5.3:17092808-17092827 MS.gene028035:CDS 20.0%
!!! ATTGTTTATTTTGATCAAGA+TGG - chr5.3:17092260-17092279 MS.gene028035:CDS 20.0%
!!! ATTGTTTCTTTTTAGGTTTA+TGG - chr5.3:17091733-17091752 MS.gene028035:CDS 20.0%
!!! TGTTTTCCTAGATTTTTTTA+AGG - chr5.3:17091695-17091714 MS.gene028035:CDS 20.0%
!!! TTTTTACCTTTCATAATTGA+AGG + chr5.3:17091925-17091944 None:intergenic 20.0%
! AAACCATATAAATGGAACAT+CGG + chr5.3:17091333-17091352 None:intergenic 25.0%
! ACCTGTATAAAATCAACATT+AGG + chr5.3:17091609-17091628 None:intergenic 25.0%
! ACTTGAATCAATGTTAGTAT+TGG - chr5.3:17092774-17092793 MS.gene028035:CDS 25.0%
! AGAGAGATAAACCATATAAA+TGG + chr5.3:17091341-17091360 None:intergenic 25.0%
! AGGATAACATGGTTATATAA+AGG - chr5.3:17091989-17092008 MS.gene028035:CDS 25.0%
! ATAACACCTAATAGTACAAT+GGG + chr5.3:17090898-17090917 None:intergenic 25.0%
! ATGGGATAGTAAATTGAATT+TGG + chr5.3:17090880-17090899 None:intergenic 25.0%
! CCTGTATAAAATCAACATTA+GGG + chr5.3:17091608-17091627 None:intergenic 25.0%
! CTTAGGATACTTGAATAATA+AGG - chr5.3:17092187-17092206 MS.gene028035:CDS 25.0%
! GTGTATACTATTGTAAATTC+CGG + chr5.3:17090653-17090672 None:intergenic 25.0%
! TAACAACTTTGAGTAAGAAA+TGG + chr5.3:17090782-17090801 None:intergenic 25.0%
! TCAAGTATCCTAAGAAATTA+AGG + chr5.3:17092181-17092200 None:intergenic 25.0%
! TGAAATTGATGAGACTATTA+GGG - chr5.3:17091413-17091432 MS.gene028035:intron 25.0%
! TTGAAATTGATGAGACTATT+AGG - chr5.3:17091412-17091431 MS.gene028035:intron 25.0%
!! AATCAATGTTAGTATTGGAA+TGG - chr5.3:17092779-17092798 MS.gene028035:CDS 25.0%
!! TCTAATTTCTTAGCATTTTC+AGG + chr5.3:17091021-17091040 None:intergenic 25.0%
!! TGTTTAATAACCAGATTTTG+AGG + chr5.3:17091147-17091166 None:intergenic 25.0%
!! TTTATAGCTTTTGTGCTTAA+AGG + chr5.3:17091567-17091586 None:intergenic 25.0%
!!! TGTTTTTTTAACCTGATTTC+TGG - chr5.3:17091108-17091127 MS.gene028035:intron 25.0%
AAACAAAGTTACCAGAAATC+AGG + chr5.3:17091122-17091141 None:intergenic 30.0%
AAAGTGGTATATTGGAATAG+TGG - chr5.3:17091853-17091872 MS.gene028035:intron 30.0%
AATCAATCTCATGCTAATGT+AGG + chr5.3:17092040-17092059 None:intergenic 30.0%
ATGTCGACAATTGATAAAGA+TGG - chr5.3:17092218-17092237 MS.gene028035:CDS 30.0%
ATTTCATCAACACTACTAAG+AGG + chr5.3:17090952-17090971 None:intergenic 30.0%
CATAACACCTAATAGTACAA+TGG + chr5.3:17090899-17090918 None:intergenic 30.0%
GAAATAATAGGAATGCCTAA+TGG + chr5.3:17091045-17091064 None:intergenic 30.0%
GCACATCCTTAAAAAAATCT+AGG + chr5.3:17091704-17091723 None:intergenic 30.0%
GCTAAGAAATTAGAACCATT+AGG - chr5.3:17091027-17091046 MS.gene028035:CDS 30.0%
GGAAAATGATGAAACACTTA+AGG + chr5.3:17092589-17092608 None:intergenic 30.0%
GTTACCTTTAGTCAAATCAA+TGG - chr5.3:17090798-17090817 MS.gene028035:intron 30.0%
TACTATCCCATTGTACTATT+AGG - chr5.3:17090889-17090908 MS.gene028035:CDS 30.0%
TCATAGAAATCTTATTGCCA+TGG - chr5.3:17091233-17091252 MS.gene028035:CDS 30.0%
TTCAGGATGCAAAATTAACA+CGG + chr5.3:17092482-17092501 None:intergenic 30.0%
TTCATATACTTACCCTGTAA+CGG + chr5.3:17092518-17092537 None:intergenic 30.0%
! AACAGCTAGATACACATTTT+TGG + chr5.3:17091307-17091326 None:intergenic 30.0%
! AATTTTGCATCCTGAAATAG+TGG - chr5.3:17092486-17092505 MS.gene028035:CDS 30.0%
! ATCGAATCCTTTTCATTTTC+TGG + chr5.3:17091072-17091091 None:intergenic 30.0%
! CTATTGTAAATTCCGGTTTT+TGG + chr5.3:17090646-17090665 None:intergenic 30.0%
!! TAAAGGTTTTGTCTATTGTG+TGG - chr5.3:17092006-17092025 MS.gene028035:CDS 30.0%
!! TTTTCTGGCACAGAAATAAT+AGG + chr5.3:17091057-17091076 None:intergenic 30.0%
!! TTTTTGGTGAATACCAAGAA+GGG + chr5.3:17090630-17090649 None:intergenic 30.0%
!!! CCCTAATGTTGATTTTATAC+AGG - chr5.3:17091605-17091624 MS.gene028035:CDS 30.0%
AATTGGAATAGCAACAAGAC+AGG + chr5.3:17092136-17092155 None:intergenic 35.0%
ACAATAGTATACACTCTCCT+AGG - chr5.3:17090659-17090678 MS.gene028035:CDS 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr5.3:17090943-17090962 None:intergenic 35.0%
ACGAAATCGATAACATCCTT+TGG + chr5.3:17092691-17092710 None:intergenic 35.0%
AGAGGTCCTTCAATTATGAA+AGG - chr5.3:17091916-17091935 MS.gene028035:CDS 35.0%
CATAAAATGGGAGTTTCACA+AGG - chr5.3:17090832-17090851 MS.gene028035:CDS 35.0%
CGACAATTGATAAAGATGGT+TGG - chr5.3:17092222-17092241 MS.gene028035:CDS 35.0%
GTTCTTCAATCGTTGTAATG+AGG - chr5.3:17091385-17091404 MS.gene028035:intron 35.0%
TATCAAATACAAGGGCTTTC+AGG - chr5.3:17092316-17092335 MS.gene028035:CDS 35.0%
TCTTGGTATTCACCAAAAAC+CGG - chr5.3:17090631-17090650 MS.gene028035:CDS 35.0%
TGGTTTATCTCTCTTTGCTA+CGG - chr5.3:17091347-17091366 MS.gene028035:intron 35.0%
TTAGTCAAATCAATGGCTTC+TGG - chr5.3:17090805-17090824 MS.gene028035:intron 35.0%
TTCGCAACTATTCTTCACTA+GGG - chr5.3:17091806-17091825 MS.gene028035:intron 35.0%
TTCTGTGCCAGAAAATGAAA+AGG - chr5.3:17091062-17091081 MS.gene028035:intron 35.0%
TTGCCGATGTTCCATTTATA+TGG - chr5.3:17091327-17091346 MS.gene028035:intron 35.0%
TTGGAAGTAGAAGCAAAACA+AGG + chr5.3:17090861-17090880 None:intergenic 35.0%
TTTCGCAACTATTCTTCACT+AGG - chr5.3:17091805-17091824 MS.gene028035:intron 35.0%
! CTATTAGGTGTTATGTACCT+AGG - chr5.3:17090904-17090923 MS.gene028035:intron 35.0%
! GCTGCTATATTGTTTTCTTC+TGG - chr5.3:17091177-17091196 MS.gene028035:intron 35.0%
! GCTTCTGGTCTTCATAAAAT+GGG - chr5.3:17090820-17090839 MS.gene028035:CDS 35.0%
! TGTTGATGAAATTCGTAAGC+AGG - chr5.3:17090960-17090979 MS.gene028035:intron 35.0%
! TTGTTTTCTTCTGGTACTAC+TGG - chr5.3:17091186-17091205 MS.gene028035:intron 35.0%
! TTTTCCATATATCAGTGCCA+AGG - chr5.3:17092603-17092622 MS.gene028035:CDS 35.0%
!! GAAGCCATTGATTTGACTAA+AGG + chr5.3:17090805-17090824 None:intergenic 35.0%
!!! ATTTTTTCTCACCCTCATGA+TGG - chr5.3:17090721-17090740 MS.gene028035:intron 35.0%
!!! CTTTGTTTTGCCTCAAAATC+TGG - chr5.3:17091134-17091153 MS.gene028035:intron 35.0%
!!! GTTTTTGGTGAATACCAAGA+AGG + chr5.3:17090631-17090650 None:intergenic 35.0%
!!! TTAACCCTTTTTTTGTTGGC+AGG - chr5.3:17092812-17092831 MS.gene028035:CDS 35.0%
!!! TTTTTTCTCACCCTCATGAT+GGG - chr5.3:17090722-17090741 MS.gene028035:intron 35.0%
AACGATACAACGTCGAGAAA+AGG + chr5.3:17090700-17090719 None:intergenic 40.0%
AATCAACATTAGGGAGAGCT+TGG + chr5.3:17091599-17091618 None:intergenic 40.0%
ACGATACAACGTCGAGAAAA+GGG + chr5.3:17090699-17090718 None:intergenic 40.0%
ACTTTGCGAACCTTCTTGAA+TGG + chr5.3:17092843-17092862 None:intergenic 40.0%
AGCAACCTGCCAACAAAAAA+AGG + chr5.3:17092820-17092839 None:intergenic 40.0%
ATATCATCACTCACCCTTCT+TGG - chr5.3:17090614-17090633 MS.gene028035:CDS 40.0%
ATTCAAGAAGGTTCGCAAAG+TGG - chr5.3:17092842-17092861 MS.gene028035:CDS 40.0%
CCCAGAAGAGGAATCAATAA+GGG + chr5.3:17090755-17090774 None:intergenic 40.0%
GCAACCTGCCAACAAAAAAA+GGG + chr5.3:17092819-17092838 None:intergenic 40.0%
GCTACGGGATTATTGTCATT+GGG - chr5.3:17091363-17091382 MS.gene028035:intron 40.0%
GGTTTATCTCTCTTTGCTAC+GGG - chr5.3:17091348-17091367 MS.gene028035:intron 40.0%
GGTTTATGGAATGACTGAGT+CGG - chr5.3:17091747-17091766 MS.gene028035:CDS 40.0%
GTTTATGGAATGACTGAGTC+GGG - chr5.3:17091748-17091767 MS.gene028035:CDS 40.0%
TCCCAGAAGAGGAATCAATA+AGG + chr5.3:17090756-17090775 None:intergenic 40.0%
TCTTGTTCCACCGATATTGT+CGG - chr5.3:17091467-17091486 MS.gene028035:intron 40.0%
TGCTACGGGATTATTGTCAT+TGG - chr5.3:17091362-17091381 MS.gene028035:intron 40.0%
TGGAGGCAAAAGTGGTATAT+TGG - chr5.3:17091845-17091864 MS.gene028035:intron 40.0%
! AAAGATGGTTGGCTACATAC+TGG - chr5.3:17092233-17092252 MS.gene028035:CDS 40.0%
! AGCAGTTTCTGAGTTGCTTT+CGG + chr5.3:17092911-17092930 None:intergenic 40.0%
! GGCTTCTGGTCTTCATAAAA+TGG - chr5.3:17090819-17090838 MS.gene028035:CDS 40.0%
! GGGTGAGTGATGATATTTTG+TGG + chr5.3:17090610-17090629 None:intergenic 40.0%
! TAAGTTGCAGAGTTTGAGAC+AGG - chr5.3:17091524-17091543 MS.gene028035:intron 40.0%
! TATTGCCATGGTTGAGCTTT+TGG - chr5.3:17091245-17091264 MS.gene028035:CDS 40.0%
! TGGAAGTGTACTATCTCCAA+AGG - chr5.3:17092672-17092691 MS.gene028035:CDS 40.0%
! TTTTTCTCACCCTCATGATG+GGG - chr5.3:17090723-17090742 MS.gene028035:intron 40.0%
!! CCCTTATTGATTCCTCTTCT+GGG - chr5.3:17090752-17090771 MS.gene028035:intron 40.0%
!! TATCGATTTCGTTGCTAAGC+AGG - chr5.3:17092699-17092718 MS.gene028035:CDS 40.0%
!! TCATCCTTGGCACTGATATA+TGG + chr5.3:17092610-17092629 None:intergenic 40.0%
AACACGGCTTCTAAATCAGC+AGG + chr5.3:17092466-17092485 None:intergenic 45.0%
ACAGGCAGAAAAGGTTCAAG+TGG + chr5.3:17092118-17092137 None:intergenic 45.0%
AGAAATGGAAGTCCCAGAAG+AGG + chr5.3:17090767-17090786 None:intergenic 45.0%
AGCAACAAGACAGGCAGAAA+AGG + chr5.3:17092127-17092146 None:intergenic 45.0%
AGGGTTGTTAGCTCCAAACA+TGG - chr5.3:17091825-17091844 MS.gene028035:intron 45.0%
AGTGGGATTTGTGGTCAAGA+AGG - chr5.3:17092648-17092667 MS.gene028035:CDS 45.0%
ATGGTGTAACAACAGCACCT+AGG + chr5.3:17090924-17090943 None:intergenic 45.0%
CGTAAGCAGGTTAATGACTG+TGG - chr5.3:17090973-17090992 MS.gene028035:intron 45.0%
GAAAAGGTTCAAGTGGCTTG+AGG + chr5.3:17092111-17092130 None:intergenic 45.0%
GCTGTCAATGCCGACAATAT+CGG + chr5.3:17091480-17091499 None:intergenic 45.0%
GGATTTGTGGTCAAGAAGGT+TGG - chr5.3:17092652-17092671 MS.gene028035:CDS 45.0%
GTTGTTAGCTCCAAACATGG+AGG - chr5.3:17091828-17091847 MS.gene028035:intron 45.0%
TACAACGTCGAGAAAAGGGT+CGG + chr5.3:17090695-17090714 None:intergenic 45.0%
TCCAAACATGGAGGCAAAAG+TGG - chr5.3:17091837-17091856 MS.gene028035:intron 45.0%
TTCTTGACCACAAATCCCAC+TGG + chr5.3:17092649-17092668 None:intergenic 45.0%
! AGTTTCTGAGTTGCTTTCGG+AGG + chr5.3:17092908-17092927 None:intergenic 45.0%
! TTGCAGAGTTTGAGACAGGT+CGG - chr5.3:17091528-17091547 MS.gene028035:intron 45.0%
!! ACCACTTTTGCCTCCATGTT+TGG + chr5.3:17091841-17091860 None:intergenic 45.0%
!! GCCCTTATTGATTCCTCTTC+TGG - chr5.3:17090751-17090770 MS.gene028035:intron 45.0%
!! TAATAAATATAAATACTAAA+AGG - chr5.3:17091969-17091988 MS.gene028035:CDS 5.0%
AAAGCTCACCACTTCTGCCT+GGG + chr5.3:17091891-17091910 None:intergenic 50.0%
AAGATTCCGAGGTCTGTGAC+TGG - chr5.3:17092876-17092895 MS.gene028035:CDS 50.0%
ACGGCGACATCCACTATTTC+AGG + chr5.3:17092499-17092518 None:intergenic 50.0%
ACGGCTTCTAAATCAGCAGG+AGG + chr5.3:17092463-17092482 None:intergenic 50.0%
AGATTCCGAGGTCTGTGACT+GGG - chr5.3:17092877-17092896 MS.gene028035:CDS 50.0%
AGGGCTAAAACCCCATCATG+AGG + chr5.3:17090736-17090755 None:intergenic 50.0%
AGTGCCAAGGATGAAGAAGC+TGG - chr5.3:17092616-17092635 MS.gene028035:CDS 50.0%
ATCTTCCCAGTCACAGACCT+CGG + chr5.3:17092885-17092904 None:intergenic 50.0%
ATGACTGAGTCGGGTGCATT+AGG - chr5.3:17091757-17091776 MS.gene028035:CDS 50.0%
CACAGACCTCGGAATCTTGT+CGG + chr5.3:17092874-17092893 None:intergenic 50.0%
GCAGGTTGCTCCATTCAAGA+AGG - chr5.3:17092830-17092849 MS.gene028035:CDS 50.0%
GGAGATTCCAGTGGGATTTG+TGG - chr5.3:17092639-17092658 MS.gene028035:CDS 50.0%
GGGCTAAAACCCCATCATGA+GGG + chr5.3:17090735-17090754 None:intergenic 50.0%
GGTACTACTGGTGTGAGCAA+AGG - chr5.3:17091198-17091217 MS.gene028035:CDS 50.0%
GTCAATGCCGACAATATCGG+TGG + chr5.3:17091477-17091496 None:intergenic 50.0%
GTGCCAAGGATGAAGAAGCT+GGG - chr5.3:17092617-17092636 MS.gene028035:CDS 50.0%
TCTTCACCGACAAGATTCCG+AGG - chr5.3:17092865-17092884 MS.gene028035:CDS 50.0%
TGCCAAGGATGAAGAAGCTG+GGG - chr5.3:17092618-17092637 MS.gene028035:CDS 50.0%
! AGTGGTGAGCTTTGGTTGAG+AGG - chr5.3:17091898-17091917 MS.gene028035:intron 50.0%
! GCATTGACAGCGAAGGCAAA+GGG - chr5.3:17091489-17091508 MS.gene028035:intron 50.0%
! GCGAAGGCAAAGGGTGTAAA+TGG - chr5.3:17091498-17091517 MS.gene028035:intron 50.0%
! TAGTGGATGTCGCCGTTACA+GGG - chr5.3:17092503-17092522 MS.gene028035:CDS 50.0%
!! ATAGTGGATGTCGCCGTTAC+AGG - chr5.3:17092502-17092521 MS.gene028035:CDS 50.0%
!! ATTGTCGGCATTGACAGCGA+AGG - chr5.3:17091482-17091501 MS.gene028035:intron 50.0%
AAGCTCACCACTTCTGCCTG+GGG + chr5.3:17091890-17091909 None:intergenic 55.0%
ACGTCGAGAAAAGGGTCGGT+CGG + chr5.3:17090691-17090710 None:intergenic 55.0%
AGAAGCTGGGGAGATTCCAG+TGG - chr5.3:17092630-17092649 MS.gene028035:CDS 55.0%
CAAAGCTCACCACTTCTGCC+TGG + chr5.3:17091892-17091911 None:intergenic 55.0%
CAGGCAGAAGTGGTGAGCTT+TGG - chr5.3:17091890-17091909 MS.gene028035:intron 55.0%
CCTCGCCAAAAGCTCAACCA+TGG + chr5.3:17091253-17091272 None:intergenic 55.0%
CTCCCCAGCTTCTTCATCCT+TGG + chr5.3:17092623-17092642 None:intergenic 55.0%
GAAGCTGGGGAGATTCCAGT+GGG - chr5.3:17092631-17092650 MS.gene028035:CDS 55.0%
! CCATGGTTGAGCTTTTGGCG+AGG - chr5.3:17091250-17091269 MS.gene028035:CDS 55.0%
! TCGGGTGCATTAGGAGCATG+CGG - chr5.3:17091766-17091785 MS.gene028035:intron 55.0%
! TTTTTGCCCCCAGGCAGAAG+TGG - chr5.3:17091880-17091899 MS.gene028035:intron 55.0%
!! GGCATTGACAGCGAAGGCAA+AGG - chr5.3:17091488-17091507 MS.gene028035:intron 55.0%
AGCTCACCACTTCTGCCTGG+GGG + chr5.3:17091889-17091908 None:intergenic 60.0%
CGAGAAAAGGGTCGGTCGGA+AGG + chr5.3:17090687-17090706 None:intergenic 60.0%
! AGTGGTGCGTTTTTGCCCCC+AGG - chr5.3:17091871-17091890 MS.gene028035:intron 60.0%
GGGTCGGTCGGAAGGCTCCT+AGG + chr5.3:17090679-17090698 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 17090580 17092947 17090580 ID=MS.gene028035
chr5.3 mRNA 17090580 17092947 17090580 ID=MS.gene028035.t1;Parent=MS.gene028035
chr5.3 exon 17091901 17092947 17091901 ID=MS.gene028035.t1.exon1;Parent=MS.gene028035.t1
chr5.3 CDS 17091901 17092947 17091901 ID=cds.MS.gene028035.t1;Parent=MS.gene028035.t1
chr5.3 exon 17091590 17091779 17091590 ID=MS.gene028035.t1.exon2;Parent=MS.gene028035.t1
chr5.3 CDS 17091590 17091779 17091590 ID=cds.MS.gene028035.t1;Parent=MS.gene028035.t1
chr5.3 exon 17091190 17091335 17091190 ID=MS.gene028035.t1.exon3;Parent=MS.gene028035.t1
chr5.3 CDS 17091190 17091335 17091190 ID=cds.MS.gene028035.t1;Parent=MS.gene028035.t1
chr5.3 exon 17091003 17091070 17091003 ID=MS.gene028035.t1.exon4;Parent=MS.gene028035.t1
chr5.3 CDS 17091003 17091070 17091003 ID=cds.MS.gene028035.t1;Parent=MS.gene028035.t1
chr5.3 exon 17090807 17090909 17090807 ID=MS.gene028035.t1.exon5;Parent=MS.gene028035.t1
chr5.3 CDS 17090807 17090909 17090807 ID=cds.MS.gene028035.t1;Parent=MS.gene028035.t1
chr5.3 exon 17090580 17090693 17090580 ID=MS.gene028035.t1.exon6;Parent=MS.gene028035.t1
chr5.3 CDS 17090580 17090693 17090580 ID=cds.MS.gene028035.t1;Parent=MS.gene028035.t1
Gene Sequence

>MS.gene028035

ATGGCTGCAAATTTAAACTATGACATGCCACAAAATATCATCACTCACCCTTCTTGGTATTCACCAAAAACCGGAATTTACAATAGTATACACTCTCCTAGGAGCCTTCCGACCGACCCTTTTCTCGACGTTGTATCGTTCATTTTTTCTCACCCTCATGATGGGGTTTTAGCCCTTATTGATTCCTCTTCTGGGACTTCCATTTCTTACTCAAAGTTGTTACCTTTAGTCAAATCAATGGCTTCTGGTCTTCATAAAATGGGAGTTTCACAAGGTGACCTTGTTTTGCTTCTACTTCCAAATTCAATTTACTATCCCATTGTACTATTAGGTGTTATGTACCTAGGTGCTGTTGTTACACCATTGAATCCTCTTAGTAGTGTTGATGAAATTCGTAAGCAGGTTAATGACTGTGGTGTGAGTTTTGCTTTTACTATACCTGAAAATGCTAAGAAATTAGAACCATTAGGCATTCCTATTATTTCTGTGCCAGAAAATGAAAAGGATTCGATGAATGATTGTTTCTCATGTTTTTTTAACCTGATTTCTGGTAACTTTGTTTTGCCTCAAAATCTGGTTATTAAACAAGAAGACACTGCTGCTATATTGTTTTCTTCTGGTACTACTGGTGTGAGCAAAGGAGTTGTTTTAACTCATAGAAATCTTATTGCCATGGTTGAGCTTTTGGCGAGGTTTGAAACTTCTAAATATGAATGCTCTAGTTCCAAAAATGTGTATCTAGCTGTTTTGCCGATGTTCCATTTATATGGTTTATCTCTCTTTGCTACGGGATTATTGTCATTGGGTTCTTCAATCGTTGTAATGAGGAAATTTGAAATTGATGAGACTATTAGGGTGATCGATAAATATAAAGTTACACACTTTCATCTTGTTCCACCGATATTGTCGGCATTGACAGCGAAGGCAAAGGGTGTAAATGGAAGTAAGTTGCAGAGTTTGAGACAGGTCGGCTCTAGTGCAGCGCCTTTAAGCACAAAAGCTATAAATGATTTTGTCCAAGCTCTCCCTAATGTTGATTTTATACAGGTAAAAACCTCAGAATAAAATAGTCTTATTTAAAGAATGCAAGTCATGATCCATCATTCAAATAATGTTGTTTTCCTAGATTTTTTTAAGGATGTGCAGATGTAAAATTGTTTCTTTTTAGGTTTATGGAATGACTGAGTCGGGTGCATTAGGAGCATGCGGTTTCAATACAGAAAAATTTCGCAACTATTCTTCACTAGGGTTGTTAGCTCCAAACATGGAGGCAAAAGTGGTATATTGGAATAGTGGTGCGTTTTTGCCCCCAGGCAGAAGTGGTGAGCTTTGGTTGAGAGGTCCTTCAATTATGAAAGGTAAAAAAAATCTGCATTTTTTATGAATAGATAATAAATATAAATACTAAAAGGATAACATGGTTATATAAAGGTTTTGTCTATTGTGTGGAATCTTAACCTACATTAGCATGAGATTGATTCAATGATTTCAACTGTTTTGTTGCTGTAACTTTTGTTTCTGTCTTATTCCTCAAGCCACTTGAACCTTTTCTGCCTGTCTTGTTGCTATTCCAATTGAAATTATTAATCAAACTTTTTCCTTAATTTCTTAGGATACTTGAATAATAAGGAAGCTACAATGTCGACAATTGATAAAGATGGTTGGCTACATACTGGAGATATTGTTTATTTTGATCAAGATGGATATTTATATTTATTTGACCGCTTGAAAGAATTTATCAAATACAAGGGCTTTCAGGTATCAATTTTTTTGTTTATTTAAATTTTATAGTGATGTTACATATTTTTATTTTACAGTTTATTTTTACTCAATTAGTATGCATTGTTCTTATTTATATCACACATGATCTGTTACAGATTCCTCCTGCTGATTTAGAAGCCGTGTTAATTTTGCATCCTGAAATAGTGGATGTCGCCGTTACAGGGTAAGTATATGAACACTTCATTTATGTCTTCTAAGATGCCTCTTTAGACATTTAAATATAACCTTAAGTGTTTCATCATTTTCCATATATCAGTGCCAAGGATGAAGAAGCTGGGGAGATTCCAGTGGGATTTGTGGTCAAGAAGGTTGGAAGTGTACTATCTCCAAAGGATGTTATCGATTTCGTTGCTAAGCAGGTTCTTCTTTATAAGACAGACATGAGATCTATGATATTTGAATTAATGTCACTACTTGAATCAATGTTAGTATTGGAATGGTTTTCAAATTTTAACCCTTTTTTTGTTGGCAGGTTGCTCCATTCAAGAAGGTTCGCAAAGTGGTCTTCACCGACAAGATTCCGAGGTCTGTGACTGGGAAGATCCTCCGAAAGCAACTCAGAAACTGCTTGCCTTCTAAACTCTAA
Protein sequence

>MS.gene028035.t1

MAANLNYDMPQNIITHPSWYSPKTGIYNSIHSPRSLPTDPFLDVVSFIFSHPHDGVLALIDSSSGTSISYSKLLPLVKSMASGLHKMGVSQGDLVLLLLPNSIYYPIVLLGVMYLGAVVTPLNPLSSVDEIRKQVNDCGVSFAFTIPENAKKLEPLGIPIISVPENEKDSMNDCFSCFFNLISGNFVLPQNLVIKQEDTAAILFSSGTTGVSKGVVLTHRNLIAMVELLARFETSKYECSSSKNVYLAVLPMFHLYGLSLFATGLLSLGSSIVVMRKFEIDETIRVIDKYKVTHFHLVPPILSALTAKAKGVNGSKLQSLRQVGSSAAPLSTKAINDFVQALPNVDFIQVYGMTESGALGACGFNTEKFRNYSSLGLLAPNMEAKVVYWNSGAFLPPGRSGELWLRGPSIMKGYLNNKEATMSTIDKDGWLHTGDIVYFDQDGYLYLFDRLKEFIKYKGFQIPPADLEAVLILHPEIVDVAVTGAKDEEAGEIPVGFVVKKVGSVLSPKDVIDFVAKQVAPFKKVRKVVFTDKIPRSVTGKILRKQLRNCLPSKL