AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030325

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030325.t1 MTR_4g077900 97.699 478 11 0 86 563 1 478 0.0 952
MS.gene030325.t1 MTR_5g027480 79.464 560 109 2 4 563 2 555 0.0 909
MS.gene030325.t1 MTR_5g027500 79.021 572 103 3 4 563 2 568 0.0 892
MS.gene030325.t1 MTR_1g026310 45.138 545 288 5 26 563 20 560 6.17e-158 463
MS.gene030325.t1 MTR_8g039720 43.920 551 288 7 26 563 17 559 6.43e-152 447
MS.gene030325.t1 MTR_1g090737 44.964 556 276 12 26 563 27 570 5.80e-141 420
MS.gene030325.t1 MTR_4g057730 41.651 533 295 8 31 555 10 534 2.21e-132 397
MS.gene030325.t1 MTR_3g088880 41.600 500 273 6 60 555 64 548 2.99e-130 392
MS.gene030325.t1 MTR_3g088870 43.173 498 263 9 64 555 67 550 3.45e-129 389
MS.gene030325.t1 MTR_1g063120 40.179 560 307 10 24 563 4 555 3.96e-128 386
MS.gene030325.t1 MTR_2g095510 39.706 544 303 8 31 563 10 539 7.13e-128 385
MS.gene030325.t1 MTR_1g063110 39.779 543 310 7 24 555 4 540 1.28e-122 372
MS.gene030325.t1 MTR_2g105570 39.739 536 305 10 36 561 20 547 1.18e-112 346
MS.gene030325.t1 MTR_4g057730 41.667 468 257 8 31 490 10 469 2.10e-109 335
MS.gene030325.t1 MTR_1g073180 38.889 522 280 11 48 555 30 526 5.20e-109 337
MS.gene030325.t1 MTR_4g005750 38.606 531 312 9 36 561 19 540 4.09e-102 319
MS.gene030325.t1 MTR_4g128337 38.160 511 292 10 65 563 50 548 5.35e-101 316
MS.gene030325.t1 MTR_4g057730 40.444 450 248 8 31 470 10 449 1.03e-99 310
MS.gene030325.t1 MTR_1g073180 38.730 488 265 10 60 538 69 531 1.49e-99 312
MS.gene030325.t1 MTR_3g460770 34.004 547 339 8 26 563 8 541 1.92e-98 310
MS.gene030325.t1 MTR_5g007640 38.370 503 299 7 65 561 88 585 7.93e-98 309
MS.gene030325.t1 MTR_2g095510 38.242 455 256 8 31 474 10 450 2.26e-94 296
MS.gene030325.t1 MTR_1g026470 39.119 386 224 5 26 404 20 401 1.77e-81 261
MS.gene030325.t1 MTR_1g026430 39.119 386 224 5 26 404 20 401 3.79e-81 260
MS.gene030325.t1 MTR_3g035130 30.862 499 301 15 74 553 32 505 5.89e-53 188
MS.gene030325.t1 MTR_1569s0010 27.413 518 330 12 74 556 22 528 6.40e-44 164
MS.gene030325.t1 MTR_5g076240 28.752 553 337 17 41 557 11 542 6.85e-44 164
MS.gene030325.t1 MTR_3g035130 30.684 453 270 15 74 507 32 459 5.06e-42 157
MS.gene030325.t1 MTR_3g064420 27.154 534 320 16 70 556 36 547 1.92e-39 152
MS.gene030325.t1 MTR_0803s0020 28.656 506 295 16 76 549 263 734 5.06e-39 153
MS.gene030325.t1 MTR_4g011790 28.389 391 247 10 196 562 217 598 4.81e-38 148
MS.gene030325.t1 MTR_5g076220 27.810 525 325 16 70 557 36 543 5.01e-38 148
MS.gene030325.t1 MTR_4g013210 29.455 404 242 13 192 562 52 445 1.73e-37 144
MS.gene030325.t1 MTR_4g011790 28.496 379 238 10 196 550 217 586 5.75e-36 142
MS.gene030325.t1 MTR_0161s0050 28.686 373 230 9 204 556 188 544 6.25e-36 142
MS.gene030325.t1 MTR_4g011790 27.750 400 247 12 196 562 217 607 1.34e-35 141
MS.gene030325.t1 MTR_8g074040 28.000 500 315 17 86 556 94 577 2.86e-34 137
MS.gene030325.t1 MTR_5g076200 26.577 523 332 16 70 556 36 542 2.60e-33 134
MS.gene030325.t1 MTR_5g008820 27.723 505 306 18 86 556 52 531 4.32e-32 130
MS.gene030325.t1 MTR_5g023730 26.112 517 330 17 75 561 48 542 9.33e-32 129
MS.gene030325.t1 MTR_2g030240 25.865 549 313 16 77 563 31 547 8.16e-31 127
MS.gene030325.t1 MTR_8g074040 28.646 384 235 13 204 563 106 474 1.46e-30 125
MS.gene030325.t1 MTR_2g044180 29.891 368 219 11 212 556 110 461 1.68e-30 125
MS.gene030325.t1 MTR_2g044180 29.891 368 219 11 212 556 201 552 3.08e-30 125
MS.gene030325.t1 MTR_4g013210 28.954 373 222 12 192 531 204 566 3.43e-30 125
MS.gene030325.t1 MTR_5g008800 27.309 498 321 15 86 557 52 534 4.08e-30 124
MS.gene030325.t1 MTR_4g013210 27.157 394 223 11 192 562 52 404 1.10e-29 121
MS.gene030325.t1 MTR_4g013210 26.650 394 225 10 192 562 204 556 3.60e-29 122
MS.gene030325.t1 MTR_8g074120 27.200 375 228 11 206 557 137 489 4.71e-29 121
MS.gene030325.t1 MTR_4g121600 25.427 527 327 17 75 556 47 552 2.23e-28 119
MS.gene030325.t1 MTR_1g026350 41.791 134 78 0 26 159 20 153 3.09e-28 111
MS.gene030325.t1 MTR_2g030240 26.598 391 236 10 210 563 77 453 2.18e-26 112
MS.gene030325.t1 MTR_1g104810 24.528 530 308 15 27 480 55 568 2.30e-24 108
MS.gene030325.t1 MTR_3g087980 26.337 486 270 18 74 483 106 579 4.08e-24 107
MS.gene030325.t1 MTR_3g084450 26.055 545 293 18 63 503 94 632 2.07e-23 105
MS.gene030325.t1 MTR_1g104810 27.865 384 211 12 146 480 135 501 3.28e-23 104
MS.gene030325.t1 MTR_7g109830 26.062 518 307 18 43 490 36 547 2.92e-22 101
MS.gene030325.t1 MTR_1g030290 29.619 341 172 14 206 485 250 583 5.06e-22 100
MS.gene030325.t1 MTR_8g083170 25.901 583 320 20 63 540 96 671 9.32e-22 100
MS.gene030325.t1 MTR_7g084850 24.440 536 349 16 72 563 201 724 1.30e-21 99.8
MS.gene030325.t1 MTR_3g084450 27.229 415 212 14 168 503 21 424 1.52e-21 98.6
MS.gene030325.t1 MTR_1g086600 24.517 518 317 14 43 490 58 571 5.72e-21 97.4
MS.gene030325.t1 MTR_5g009350 24.860 535 313 19 28 485 22 544 1.29e-20 95.9
MS.gene030325.t1 MTR_5g009350 24.860 535 313 19 28 485 22 544 1.76e-20 95.9
MS.gene030325.t1 MTR_5g009350 24.860 535 313 19 28 485 22 544 2.01e-20 95.5
MS.gene030325.t1 MTR_5g009350 24.860 535 313 19 28 485 22 544 2.14e-20 95.5
MS.gene030325.t1 MTR_4g057755 52.778 72 34 0 419 490 46 117 3.55e-20 87.0
MS.gene030325.t1 MTR_5g009360 24.597 496 298 16 56 485 59 544 1.26e-19 93.2
MS.gene030325.t1 MTR_5g009360 27.729 339 190 11 194 485 88 418 3.26e-18 88.2
MS.gene030325.t1 MTR_1054s0020 23.154 501 341 11 71 554 294 767 5.97e-18 88.2
MS.gene030325.t1 MTR_3g082660 27.811 338 166 14 208 479 284 609 4.72e-17 85.1
MS.gene030325.t1 MTR_4g013210 26.821 302 167 9 192 480 204 464 4.76e-17 84.7
MS.gene030325.t1 MTR_3g084470 30.544 239 135 8 305 528 419 641 1.63e-16 83.6
MS.gene030325.t1 MTR_3g088890 50.794 63 31 0 465 527 10 72 4.47e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030325.t1 AT4G19010 59.640 555 215 5 15 563 11 562 0.0 660
MS.gene030325.t1 AT1G20510 44.630 540 288 5 26 561 10 542 4.39e-161 471
MS.gene030325.t1 AT1G20480 42.077 549 297 7 26 563 27 565 2.41e-149 441
MS.gene030325.t1 AT1G20500 42.210 552 297 7 21 563 12 550 1.76e-146 433
MS.gene030325.t1 AT4G05160 40.183 545 308 6 29 563 8 544 2.32e-134 402
MS.gene030325.t1 AT5G38120 40.541 555 301 9 21 563 13 550 6.56e-134 401
MS.gene030325.t1 AT5G63380 39.817 545 302 8 26 555 17 550 1.48e-133 400
MS.gene030325.t1 AT1G20510 43.041 467 259 3 26 490 10 471 1.38e-132 395
MS.gene030325.t1 AT1G20510 42.094 449 249 5 26 470 10 451 2.87e-120 363
MS.gene030325.t1 AT5G38120 39.306 519 286 9 21 527 13 514 2.78e-118 360
MS.gene030325.t1 AT3G21240 39.085 568 317 12 11 563 1 554 4.95e-113 347
MS.gene030325.t1 AT5G38120 39.419 482 263 9 21 490 13 477 7.69e-108 332
MS.gene030325.t1 AT5G38120 39.419 482 263 9 21 490 13 477 7.69e-108 332
MS.gene030325.t1 AT1G51680 41.176 510 284 6 65 563 57 561 1.64e-106 331
MS.gene030325.t1 AT1G65060 39.483 542 308 10 21 556 30 557 4.69e-105 327
MS.gene030325.t1 AT3G21230 38.053 565 332 8 7 561 10 566 6.00e-104 325
MS.gene030325.t1 AT1G20490 39.161 429 241 8 21 440 12 429 2.65e-93 293
MS.gene030325.t1 AT1G20490 39.161 429 241 8 21 440 12 429 2.65e-93 293
MS.gene030325.t1 AT3G21230 37.248 545 320 9 7 541 10 542 4.73e-93 296
MS.gene030325.t1 AT1G62940 37.132 509 297 9 63 561 43 538 9.83e-93 295
MS.gene030325.t1 AT1G51680 40.084 474 268 6 65 527 57 525 2.16e-91 291
MS.gene030325.t1 AT3G21240 37.626 497 281 12 11 492 1 483 1.34e-86 277
MS.gene030325.t1 AT3G21240 37.626 497 281 12 11 492 1 483 1.34e-86 277
MS.gene030325.t1 AT3G21230 36.895 496 295 8 7 492 10 497 7.31e-82 265
MS.gene030325.t1 AT1G51680 39.408 439 250 6 65 492 57 490 1.07e-80 261
MS.gene030325.t1 AT1G65060 36.853 464 261 11 21 472 30 473 2.24e-72 240
MS.gene030325.t1 AT3G48990 28.747 487 329 11 73 553 30 504 4.40e-49 178
MS.gene030325.t1 AT3G21230 32.235 425 270 8 7 421 10 426 2.66e-45 166
MS.gene030325.t1 AT1G77240 29.885 522 312 19 73 561 43 543 4.75e-44 164
MS.gene030325.t1 AT5G16370 29.941 511 295 19 86 557 53 539 1.16e-43 164
MS.gene030325.t1 AT1G21540 27.042 551 345 19 47 561 19 548 2.27e-42 160
MS.gene030325.t1 AT1G21530 27.938 519 321 19 73 557 44 543 8.37e-41 155
MS.gene030325.t1 AT1G65890 27.451 510 319 17 86 556 52 549 1.79e-40 155
MS.gene030325.t1 AT5G16340 29.020 510 299 18 86 556 53 538 4.05e-40 154
MS.gene030325.t1 AT1G21530 27.853 517 322 19 73 557 44 541 1.68e-39 152
MS.gene030325.t1 AT1G65880 26.641 518 314 16 86 556 52 550 2.73e-38 149
MS.gene030325.t1 AT1G20560 28.827 503 309 15 86 556 54 539 7.01e-37 144
MS.gene030325.t1 AT1G75960 29.273 509 301 16 86 557 53 539 1.06e-36 144
MS.gene030325.t1 AT1G66120 28.287 502 318 14 86 556 52 542 3.14e-36 143
MS.gene030325.t1 AT1G30520 26.465 529 312 16 83 557 42 547 1.33e-33 135
MS.gene030325.t1 AT1G30520 26.465 529 312 16 83 557 42 547 1.33e-33 135
MS.gene030325.t1 AT1G20560 30.287 383 232 10 192 556 96 461 1.60e-32 130
MS.gene030325.t1 AT3G16170 27.577 388 237 12 205 562 234 607 2.17e-32 132
MS.gene030325.t1 AT3G16910 28.861 395 234 11 189 556 181 555 7.99e-32 130
MS.gene030325.t1 AT2G17650 26.145 524 306 18 86 556 99 594 1.44e-31 129
MS.gene030325.t1 AT1G76290 25.502 498 326 16 86 556 52 531 2.90e-27 116
MS.gene030325.t1 AT1G30520 26.067 445 263 15 83 487 42 460 1.92e-25 110
MS.gene030325.t1 AT1G30520 26.067 445 263 15 83 487 42 460 1.92e-25 110
MS.gene030325.t1 AT1G64400 25.144 521 307 16 32 478 27 538 6.38e-25 109
MS.gene030325.t1 AT5G27600 26.945 527 295 17 27 478 58 569 9.93e-25 109
MS.gene030325.t1 AT5G36880 24.528 530 356 15 72 563 196 719 2.59e-24 108
MS.gene030325.t1 AT5G36880 24.528 530 356 15 72 563 146 669 3.04e-24 107
MS.gene030325.t1 AT5G36880 24.528 530 356 15 72 563 146 669 3.04e-24 107
MS.gene030325.t1 AT5G36880 24.528 530 356 15 72 563 146 669 3.04e-24 107
MS.gene030325.t1 AT5G36880 24.528 530 356 15 72 563 146 669 3.04e-24 107
MS.gene030325.t1 AT1G68270 36.709 158 89 2 409 556 363 519 7.60e-24 105
MS.gene030325.t1 AT4G11030 23.722 489 298 15 57 478 58 538 7.50e-23 103
MS.gene030325.t1 AT3G05970 30.395 329 171 11 197 478 252 569 5.43e-21 97.8
MS.gene030325.t1 AT1G49430 25.287 522 309 21 41 490 39 551 1.07e-20 96.7
MS.gene030325.t1 AT1G77590 27.920 351 179 15 206 492 100 440 4.10e-20 94.4
MS.gene030325.t1 AT4G23850 23.608 521 315 19 32 478 27 538 6.12e-20 94.4
MS.gene030325.t1 AT1G77590 27.920 351 179 15 206 492 246 586 6.67e-20 94.4
MS.gene030325.t1 AT3G23790 32.164 171 100 5 343 501 465 631 1.18e-14 77.8

Find 126 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTGCTTTACACAAAA+TGG 0.138296 6.2:-88200473 MS.gene030325:CDS
CTATTGGCTGTTCTGTATTT+AGG 0.170770 6.2:-88200388 MS.gene030325:CDS
TTGACATTGATGAGGTTATT+AGG 0.176901 6.2:-88199874 MS.gene030325:CDS
ACAGGGTAATAAATTGAATT+TGG 0.183276 6.2:+88200415 None:intergenic
GCCCTTATTGATTCTTCATC+TGG 0.217618 6.2:-88200541 MS.gene030325:CDS
TATCAAATACAAGGGATTTC+AGG 0.243292 6.2:-88198085 MS.gene030325:intron
GTTGAGTGATCATGTTGTTT+TGG 0.245364 6.2:+88200695 None:intergenic
TCATAGAAATCTTATTTCTA+TGG 0.256021 6.2:-88200053 MS.gene030325:CDS
ACGGCTGCATCGACTATTTC+AGG 0.272026 6.2:+88197913 None:intergenic
GGGTCAGTTGGAAGATTTCT+AGG 0.289970 6.2:+88200616 None:intergenic
ATTCACAACTATTCGTCAAT+AGG 0.296417 6.2:-88199001 MS.gene030325:CDS
ATGAAGGCAAAGGGTGTTAA+TGG 0.303488 6.2:-88199788 MS.gene030325:CDS
TTATTACCCTGTTATACTAT+TGG 0.314296 6.2:-88200404 MS.gene030325:CDS
AGAACAGCCAATAGTATAAC+AGG 0.320104 6.2:+88200397 None:intergenic
TGACATTGATGAGGTTATTA+GGG 0.322063 6.2:-88199873 MS.gene030325:CDS
CAGGCAGCTGCGGTGAGCTT+TGG 0.328932 6.2:-88198916 MS.gene030325:CDS
CAAAGCTCACCGCAGCTGCC+TGG 0.332403 6.2:+88198917 None:intergenic
TTGCCAATGTTTCATGTGTA+TGG 0.342668 6.2:-88199959 MS.gene030325:CDS
ACCCAGATGAAGAATCAATA+AGG 0.350199 6.2:+88200539 None:intergenic
GCTGGTATATTGTATTCATC+TGG 0.361976 6.2:-88200109 MS.gene030325:CDS
AAAGCTCACCGCAGCTGCCT+GGG 0.370228 6.2:+88198918 None:intergenic
TTCCCCAGTTTCTTCATCTT+CGG 0.374121 6.2:+88197749 None:intergenic
ACCTGTATAAAATCAACGTT+AGG 0.376743 6.2:+88199680 None:intergenic
AGCAATGCCTGAGCTGCTTT+CGG 0.384531 6.2:+88197457 None:intergenic
AGTGCCGAAGATGAAGAAAC+TGG 0.401295 6.2:-88197753 MS.gene030325:CDS
TGCTGCGGGATTATTGTCGT+TGG 0.401502 6.2:-88199924 MS.gene030325:CDS
ACCTTCCGGACCTTCTTGTA+TGG 0.403187 6.2:+88197525 None:intergenic
TTCACAACTATTCGTCAATA+GGG 0.405454 6.2:-88199000 MS.gene030325:CDS
TGTTGTTCCACCGATGTTGT+CGG 0.411176 6.2:-88199819 MS.gene030325:CDS
TGTTGGCGAAATTCGTAAAC+AGG 0.412030 6.2:-88200332 MS.gene030325:CDS
AAGGAAACAATGTCAAGAAA+AGG 0.413052 6.2:+88200595 None:intergenic
CCCTTATTGATTCTTCATCT+GGG 0.414327 6.2:-88200540 MS.gene030325:CDS
TCAGGATGGATATTTACATT+TGG 0.418131 6.2:-88198127 MS.gene030325:CDS
GTCAAGAAACTAGAGCTATT+AGG 0.418468 6.2:-88200265 MS.gene030325:CDS
TCAAAGGGTAGTTATTAAAC+AGG 0.419883 6.2:-88200140 MS.gene030325:CDS
CTATTCGTCAATAGGGTTGT+TGG 0.424322 6.2:-88198993 MS.gene030325:CDS
GACAACATCGGTGGAACAAC+AGG 0.427365 6.2:+88199821 None:intergenic
TGTTTAATAACTACCCTTTG+AGG 0.430394 6.2:+88200142 None:intergenic
GGTTTATCGCTCTTTGCTGC+GGG 0.430597 6.2:-88199938 MS.gene030325:CDS
TAACAACTTTGAGTAAGAAA+TGG 0.436153 6.2:+88200513 None:intergenic
CAGAAAATGAGAAGGATTTG+AGG 0.445513 6.2:-88200216 MS.gene030325:CDS
GCTGCGGGATTATTGTCGTT+GGG 0.447735 6.2:-88199923 MS.gene030325:CDS
CTCAACACCCTCACCCTCAT+TGG 0.448612 6.2:-88200678 MS.gene030325:CDS
TGCGGTGAGCTTTGGTTGAG+AGG 0.454034 6.2:-88198908 MS.gene030325:CDS
GCTTCTGCTTTACACAAAAT+GGG 0.455667 6.2:-88200472 MS.gene030325:CDS
CCCAGATGAAGAATCAATAA+GGG 0.467167 6.2:+88200540 None:intergenic
AATCAAATTTACCATAAAGA+AGG 0.470429 6.2:+88200167 None:intergenic
TTGCTTGACTTCTAAACTCT+AGG 0.476441 6.2:-88197439 None:intergenic
AAGCTCACCGCAGCTGCCTG+GGG 0.478646 6.2:+88198919 None:intergenic
TAAATTTGATTTGCCTCAAA+GGG 0.485047 6.2:-88200155 MS.gene030325:CDS
GCATTTGTTGTCAAGAAGGT+TGG 0.486407 6.2:-88197717 MS.gene030325:CDS
GTAAATTTGATTTGCCTCAA+AGG 0.487083 6.2:-88200156 MS.gene030325:CDS
ACATAATCGACCACATGCTT+CGG 0.487322 6.2:+88197681 None:intergenic
TTGTATTCATCTGGGACTAC+AGG 0.489553 6.2:-88200100 MS.gene030325:CDS
ATTTCGCCAACACTACTAAG+AGG 0.489624 6.2:+88200343 None:intergenic
AACACAGCTTCTAGATCAGC+AGG 0.500308 6.2:+88197946 None:intergenic
TTCTTGACAACAAATGCGAC+TGG 0.501806 6.2:+88197723 None:intergenic
AGATCCTCCGAAAGCAGCTC+AGG 0.501984 6.2:-88197464 MS.gene030325:CDS
CACAAAATGGGTGTTTCACA+AGG 0.502968 6.2:-88200460 MS.gene030325:CDS
CGGAGACCTTGGTATCTTGT+CGG 0.503203 6.2:+88197494 None:intergenic
TGGTTTATCGCTCTTTGCTG+CGG 0.507976 6.2:-88199939 MS.gene030325:CDS
TGTCGGTGAAGAACACCTTC+CGG 0.510625 6.2:+88197511 None:intergenic
TAAGTTGCAGAGTTTGAGAC+AGG 0.511530 6.2:-88199762 MS.gene030325:CDS
AGATACCAAGGTCTCCGATT+GGG 0.512248 6.2:-88197488 MS.gene030325:CDS
AGTCGCATTTGTTGTCAAGA+AGG 0.514135 6.2:-88197721 MS.gene030325:CDS
TTGCTCCATACAAGAAGGTC+CGG 0.515213 6.2:-88197530 MS.gene030325:CDS
CGGAGGCAAAAGTGGTAGAC+TGG 0.515561 6.2:-88198961 MS.gene030325:CDS
ATCTTCCCAATCGGAGACCT+TGG 0.515866 6.2:+88197483 None:intergenic
GCGGTGAGCTTTGGTTGAGA+GGG 0.517348 6.2:-88198907 MS.gene030325:CDS
TTGAATCCTCTTAGTAGTGT+TGG 0.520706 6.2:-88200349 MS.gene030325:CDS
AGGAAACAATGTCAAGAAAA+GGG 0.521407 6.2:+88200596 None:intergenic
CTGGTATATTGTATTCATCT+GGG 0.532782 6.2:-88200108 MS.gene030325:CDS
ATGTCAAGAAAAGGGTCAGT+TGG 0.533509 6.2:+88200604 None:intergenic
AAACCATACACATGAAACAT+TGG 0.533547 6.2:+88199956 None:intergenic
TCCAAACACGGAGGCAAAAG+TGG 0.534472 6.2:-88198969 MS.gene030325:CDS
GAAGCCATGGATTTGACTAA+AGG 0.537715 6.2:+88200490 None:intergenic
ATATCAACAATTGATAAAGA+TGG 0.537763 6.2:-88198183 MS.gene030325:CDS
AAAGATGGTTGGTTACATAC+AGG 0.537881 6.2:-88198168 MS.gene030325:CDS
GTTACCTTTAGTCAAATCCA+TGG 0.539728 6.2:-88200494 MS.gene030325:CDS
AAAGTGGTAGACTGGAACAA+TGG 0.541163 6.2:-88198953 MS.gene030325:CDS
CTCAGGATACTTGAATAATG+AGG 0.541417 6.2:-88198214 MS.gene030325:intron
TTCATATACTTACGCTGTAA+CGG 0.543086 6.2:+88197894 None:intergenic
TGCTTGACTTCTAAACTCTA+GGG 0.545589 6.2:-88197438 None:intergenic
ACACTACTAAGAGGATTCAA+TGG 0.545729 6.2:+88200352 None:intergenic
TTTCGGAGGATCTTCCCAAT+CGG 0.547034 6.2:+88197474 None:intergenic
AATCAACGTTAGGGAAAGCA+TGG 0.550452 6.2:+88199690 None:intergenic
GAAAGACATTATCAAATACA+AGG 0.550958 6.2:-88198094 MS.gene030325:CDS
GGTCGATTATGTTGCTGAGC+AGG 0.550976 6.2:-88197670 MS.gene030325:intron
CAACAATTGATAAAGATGGT+TGG 0.560181 6.2:-88198179 MS.gene030325:CDS
TTTGTGTAAAGCAGAAGCCA+TGG 0.560434 6.2:+88200477 None:intergenic
CCTGTATAAAATCAACGTTA+GGG 0.562718 6.2:+88199681 None:intergenic
GGCATTGACAATGAAGGCAA+AGG 0.569984 6.2:-88199798 MS.gene030325:CDS
GCATTGACAATGAAGGCAAA+GGG 0.570042 6.2:-88199797 MS.gene030325:CDS
GAGGAAATTTGACATTGATG+AGG 0.576756 6.2:-88199882 MS.gene030325:CDS
AACAGCTAGAAACACACACT+TGG 0.579086 6.2:+88199982 None:intergenic
ATTGTCAATGCCGACAACAT+CGG 0.579863 6.2:+88199809 None:intergenic
TGCTGTGCCAGAAAATGAGA+AGG 0.586565 6.2:-88200224 MS.gene030325:CDS
AAGATACCAAGGTCTCCGAT+TGG 0.589894 6.2:-88197489 MS.gene030325:CDS
AGTTTGAGACAGGTCTCTTG+CGG 0.592614 6.2:-88199752 MS.gene030325:CDS
GATGAATACCAATGAGGGTG+AGG 0.592922 6.2:+88200670 None:intergenic
GTTGTCGGCATTGACAATGA+AGG 0.595731 6.2:-88199804 MS.gene030325:CDS
GTGCCGAAGATGAAGAAACT+GGG 0.600776 6.2:-88197752 MS.gene030325:CDS
AGGGCTAAAACCCCATCATG+AGG 0.601023 6.2:+88200559 None:intergenic
AATGCCTGAGCTGCTTTCGG+AGG 0.604997 6.2:+88197460 None:intergenic
AGCTCACCGCAGCTGCCTGG+GGG 0.606825 6.2:+88198920 None:intergenic
ATTAAACAGGAAGATACTGC+TGG 0.608838 6.2:-88200127 MS.gene030325:CDS
CGTAAACAGGTTCAAGAGTG+TGG 0.611754 6.2:-88200319 MS.gene030325:CDS
AGGGTTGTTGGCTCCAAACA+CGG 0.614077 6.2:-88198981 MS.gene030325:CDS
GGGACTACAGGTGTGAGCAA+AGG 0.615067 6.2:-88200088 MS.gene030325:CDS
AAAGACATTATCAAATACAA+GGG 0.615550 6.2:-88198093 MS.gene030325:CDS
GTCAATGCCGACAACATCGG+TGG 0.615987 6.2:+88199812 None:intergenic
AGAGGGCCTTCTATTATGAG+AGG 0.618979 6.2:-88198890 MS.gene030325:intron
GCAGGTTGCTCCATACAAGA+AGG 0.622353 6.2:-88197535 MS.gene030325:intron
TCGACTGCAGTGGGAACACG+TGG 0.624008 6.2:-88199040 MS.gene030325:CDS
GCTCTACAGTCATTGTAATG+AGG 0.624765 6.2:-88199901 MS.gene030325:CDS
ATGACTGAGTCGACTGCAGT+GGG 0.649133 6.2:-88199049 MS.gene030325:CDS
GGGCTAAAACCCCATCATGA+GGG 0.653343 6.2:+88200560 None:intergenic
TCCATACAAGAAGGTCCGGA+AGG 0.659172 6.2:-88197526 MS.gene030325:CDS
TGTACTTTCTCCGAAGCATG+TGG 0.668913 6.2:-88197691 MS.gene030325:CDS
AATGACTGAGTCGACTGCAG+TGG 0.680151 6.2:-88199050 MS.gene030325:CDS
AAGAAACTAGAGCTATTAGG+TGG 0.680245 6.2:-88200262 MS.gene030325:CDS
TGCCGAAGATGAAGAAACTG+GGG 0.685738 6.2:-88197751 MS.gene030325:CDS
ATGAATACCAATGAGGGTGA+GGG 0.691317 6.2:+88200671 None:intergenic
GAACAGCCAATAGTATAACA+GGG 0.716419 6.2:+88200398 None:intergenic
TCTTCACCGACAAGATACCA+AGG 0.749835 6.2:-88197500 MS.gene030325:CDS
GTTGTTGGCTCCAAACACGG+AGG 0.795141 6.2:-88198978 MS.gene030325:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGAAAAAAATAAAATAA+AGG + chr6.2:88200614-88200633 None:intergenic 10.0%
!! GAAGAAAAAAATAAAATAAA+GGG + chr6.2:88200613-88200632 None:intergenic 10.0%
!!! TTTATTTTATTTTTTTCTTC+TGG - chr6.2:88200613-88200632 MS.gene030325:CDS 10.0%
!! AGATATTAAAAAGTAAAACA+CGG - chr6.2:88199327-88199346 MS.gene030325:intron 15.0%
!!! GTTTGATTAACAATTTTAAT+TGG + chr6.2:88199922-88199941 None:intergenic 15.0%
!!! TTATTTTTCTTTGTTATTGT+TGG - chr6.2:88198593-88198612 MS.gene030325:intron 15.0%
!! AAAGACATTATCAAATACAA+GGG - chr6.2:88200077-88200096 MS.gene030325:CDS 20.0%
!! AATCAAATTTACCATAAAGA+AGG + chr6.2:88198006-88198025 None:intergenic 20.0%
!! ATATAGAGCAAATATAGTAA+AGG + chr6.2:88199484-88199503 None:intergenic 20.0%
!! ATATCAACAATTGATAAAGA+TGG - chr6.2:88199987-88200006 MS.gene030325:CDS 20.0%
!! ATTAAGACTATTCTATTCAA+AGG + chr6.2:88198522-88198541 None:intergenic 20.0%
!! GATATTAAAAAGTAAAACAC+GGG - chr6.2:88199328-88199347 MS.gene030325:intron 20.0%
!! TATAGAGCAAATATAGTAAA+GGG + chr6.2:88199483-88199502 None:intergenic 20.0%
!! TTAATAGCAACTGTTATATA+GGG - chr6.2:88198675-88198694 MS.gene030325:intron 20.0%
!! TTTAATAGCAACTGTTATAT+AGG - chr6.2:88198674-88198693 MS.gene030325:intron 20.0%
!!! AGATATTGTTTATTTTGATC+AGG - chr6.2:88200025-88200044 MS.gene030325:CDS 20.0%
!!! AGTTTTTTTAACCTTCTTTA+TGG - chr6.2:88197992-88198011 MS.gene030325:intron 20.0%
!!! TCATAGAAATCTTATTTCTA+TGG - chr6.2:88198117-88198136 MS.gene030325:CDS 20.0%
!!! TCATTAACATTTTACTGATA+AGG + chr6.2:88199717-88199736 None:intergenic 20.0%
! AAACTGAAGTAAATTTCTCT+TGG - chr6.2:88199804-88199823 MS.gene030325:CDS 25.0%
! AACTAACATGATATTTCTCA+AGG + chr6.2:88199535-88199554 None:intergenic 25.0%
! ACAGGGTAATAAATTGAATT+TGG + chr6.2:88197758-88197777 None:intergenic 25.0%
! ATAGAGCAAATATAGTAAAG+GGG + chr6.2:88199482-88199501 None:intergenic 25.0%
! CTTACAGCTATTAATAATCT+AGG + chr6.2:88199077-88199096 None:intergenic 25.0%
! GAAAAATGATGTAACATTTG+AGG + chr6.2:88200391-88200410 None:intergenic 25.0%
! GAAAGACATTATCAAATACA+AGG - chr6.2:88200076-88200095 MS.gene030325:CDS 25.0%
! TAACAACTTTGAGTAAGAAA+TGG + chr6.2:88197660-88197679 None:intergenic 25.0%
! TTATAATCAAATGTCCAAAG+AGG + chr6.2:88200368-88200387 None:intergenic 25.0%
! TTATTACCCTGTTATACTAT+TGG - chr6.2:88197766-88197785 MS.gene030325:intron 25.0%
!! TTTTTACCTCTCATAATAGA+AGG + chr6.2:88199289-88199308 None:intergenic 25.0%
!! TTTTTGATGAATACCAATGA+GGG + chr6.2:88197508-88197527 None:intergenic 25.0%
!!! ATTGTTTATTTTGATCAGGA+TGG - chr6.2:88200029-88200048 MS.gene030325:CDS 25.0%
!!! ATTTGTTATCGCTTAACTAA+AGG - chr6.2:88199502-88199521 MS.gene030325:intron 25.0%
!!! TAAATTTGATTTGCCTCAAA+GGG - chr6.2:88198015-88198034 MS.gene030325:intron 25.0%
!!! TGTAAGATTGTTTCTTTTCA+GGG - chr6.2:88199091-88199110 MS.gene030325:intron 25.0%
!!! TTATGATCTAGAATTTGTTG+TGG + chr6.2:88199015-88199034 None:intergenic 25.0%
!!! TTTTATTTTTTTCTTCTGGC+AGG - chr6.2:88200617-88200636 MS.gene030325:CDS 25.0%
AAAACACGGGAGTAATATAA+AGG - chr6.2:88199341-88199360 MS.gene030325:intron 30.0%
AAACCATACACATGAAACAT+TGG + chr6.2:88198217-88198236 None:intergenic 30.0%
AAATAAGCTGAACAGAACTA+AGG - chr6.2:88198868-88198887 MS.gene030325:intron 30.0%
AACTCTTTCCTTTAATTCTC+AGG - chr6.2:88199939-88199958 MS.gene030325:CDS 30.0%
AAGGAAACAATGTCAAGAAA+AGG + chr6.2:88197578-88197597 None:intergenic 30.0%
AATAAGTTGTTTAGCCAAAC+AGG + chr6.2:88198701-88198720 None:intergenic 30.0%
ACCTGTATAAAATCAACGTT+AGG + chr6.2:88198493-88198512 None:intergenic 30.0%
AGGAAACAATGTCAAGAAAA+GGG + chr6.2:88197577-88197596 None:intergenic 30.0%
ATTCACAACTATTCGTCAAT+AGG - chr6.2:88199169-88199188 MS.gene030325:intron 30.0%
CAACAATTGATAAAGATGGT+TGG - chr6.2:88199991-88200010 MS.gene030325:CDS 30.0%
CATTGGTATTCATCAAAAAC+TGG - chr6.2:88197509-88197528 MS.gene030325:CDS 30.0%
CCTGTATAAAATCAACGTTA+GGG + chr6.2:88198492-88198511 None:intergenic 30.0%
CTGGTATATTGTATTCATCT+GGG - chr6.2:88198062-88198081 MS.gene030325:intron 30.0%
GTTTGGCTAAACAACTTATT+TGG - chr6.2:88198701-88198720 MS.gene030325:intron 30.0%
TATCAAATACAAGGGATTTC+AGG - chr6.2:88200085-88200104 MS.gene030325:CDS 30.0%
TCAAAGGGTAGTTATTAAAC+AGG - chr6.2:88198030-88198049 MS.gene030325:intron 30.0%
TCAAGCAATCATATAAACGA+TGG + chr6.2:88198565-88198584 None:intergenic 30.0%
TCAAGTATCCTGAGAATTAA+AGG + chr6.2:88199950-88199969 None:intergenic 30.0%
TCAGGATGGATATTTACATT+TGG - chr6.2:88200043-88200062 MS.gene030325:CDS 30.0%
TGACATTGATGAGGTTATTA+GGG - chr6.2:88198297-88198316 MS.gene030325:intron 30.0%
TGTTTAATAACTACCCTTTG+AGG + chr6.2:88198031-88198050 None:intergenic 30.0%
TTCACAACTATTCGTCAATA+GGG - chr6.2:88199170-88199189 MS.gene030325:intron 30.0%
TTCATATACTTACGCTGTAA+CGG + chr6.2:88200279-88200298 None:intergenic 30.0%
TTGACATTGATGAGGTTATT+AGG - chr6.2:88198296-88198315 MS.gene030325:intron 30.0%
! GTTTTTGATGAATACCAATG+AGG + chr6.2:88197509-88197528 None:intergenic 30.0%
! TTTTCCCATCTAGTGAAAAA+AGG + chr6.2:88199787-88199806 None:intergenic 30.0%
!! ATTGTTTCTTTTCAGGGTTA+TGG - chr6.2:88199097-88199116 MS.gene030325:intron 30.0%
!! CTGTAAGATTGTTTCTTTTC+AGG - chr6.2:88199090-88199109 MS.gene030325:intron 30.0%
!! GATTTCAGGTATCTACTTTT+AGG - chr6.2:88200099-88200118 MS.gene030325:CDS 30.0%
!! GTAAATTTGATTTGCCTCAA+AGG - chr6.2:88198014-88198033 MS.gene030325:intron 30.0%
!! GTCTTTTTCATAAGCTATCT+TGG - chr6.2:88198834-88198853 MS.gene030325:intron 30.0%
!! TCTAGTTTCTTGACATTTTC+AGG + chr6.2:88197899-88197918 None:intergenic 30.0%
!!! TTGAGTGATCATGTTGTTTT+GGG + chr6.2:88197477-88197496 None:intergenic 30.0%
AAGAAACTAGAGCTATTAGG+TGG - chr6.2:88197908-88197927 MS.gene030325:CDS 35.0%
AATCAACCTCATGCAAATCT+AGG + chr6.2:88199392-88199411 None:intergenic 35.0%
AATTGTTTGAGCTTGTCTAC+TGG + chr6.2:88198952-88198971 None:intergenic 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr6.2:88197821-88197840 None:intergenic 35.0%
ACCCAGATGAAGAATCAATA+AGG + chr6.2:88197634-88197653 None:intergenic 35.0%
AGAACAGCCAATAGTATAAC+AGG + chr6.2:88197776-88197795 None:intergenic 35.0%
ATAGCGAACAACAACAATCA+AGG - chr6.2:88199761-88199780 MS.gene030325:CDS 35.0%
ATCAACCTCATGCAAATCTA+GGG + chr6.2:88199391-88199410 None:intergenic 35.0%
ATGATATTTCTCAAGGACAC+AGG + chr6.2:88199528-88199547 None:intergenic 35.0%
ATTAAACAGGAAGATACTGC+TGG - chr6.2:88198043-88198062 MS.gene030325:intron 35.0%
CAGAAAATGAGAAGGATTTG+AGG - chr6.2:88197954-88197973 MS.gene030325:CDS 35.0%
CAGCAAGACAAGAAAAAAGA+AGG + chr6.2:88199891-88199910 None:intergenic 35.0%
CCCAGATGAAGAATCAATAA+GGG + chr6.2:88197633-88197652 None:intergenic 35.0%
CTCAGGATACTTGAATAATG+AGG - chr6.2:88199956-88199975 MS.gene030325:CDS 35.0%
GAACAGCCAATAGTATAACA+GGG + chr6.2:88197775-88197794 None:intergenic 35.0%
GAGGAAATTTGACATTGATG+AGG - chr6.2:88198288-88198307 MS.gene030325:intron 35.0%
GCTGGTATATTGTATTCATC+TGG - chr6.2:88198061-88198080 MS.gene030325:intron 35.0%
GCTTCTGCTTTACACAAAAT+GGG - chr6.2:88197698-88197717 MS.gene030325:CDS 35.0%
GTCAAGAAACTAGAGCTATT+AGG - chr6.2:88197905-88197924 MS.gene030325:intron 35.0%
GTTACCTTTAGTCAAATCCA+TGG - chr6.2:88197676-88197695 MS.gene030325:CDS 35.0%
TTGAATCCTCTTAGTAGTGT+TGG - chr6.2:88197821-88197840 MS.gene030325:intron 35.0%
TTGCCAATGTTTCATGTGTA+TGG - chr6.2:88198211-88198230 MS.gene030325:CDS 35.0%
! AAAGATGGTTGGTTACATAC+AGG - chr6.2:88200002-88200021 MS.gene030325:CDS 35.0%
! AGAAAAAAGAAGGTACAGGT+AGG + chr6.2:88199881-88199900 None:intergenic 35.0%
! ATAAGTTTTGCGAGACTGTT+TGG + chr6.2:88198928-88198947 None:intergenic 35.0%
! ATGAGGGTGTGAAAAAATGA+AGG + chr6.2:88197597-88197616 None:intergenic 35.0%
! ATGCAAATCTAGGGTAAGAT+TGG + chr6.2:88199382-88199401 None:intergenic 35.0%
! CTATTGGCTGTTCTGTATTT+AGG - chr6.2:88197782-88197801 MS.gene030325:intron 35.0%
! CTCAAATCCTTCTCATTTTC+TGG + chr6.2:88197956-88197975 None:intergenic 35.0%
! GACAAGAAAAAAGAAGGTAC+AGG + chr6.2:88199885-88199904 None:intergenic 35.0%
! TAAGTTTTGCGAGACTGTTT+GGG + chr6.2:88198927-88198946 None:intergenic 35.0%
! TTTGCATGAGGTTGATTCAA+TGG - chr6.2:88199395-88199414 MS.gene030325:intron 35.0%
!! AAGAGGCACTTTAGAAGATT+AGG + chr6.2:88200351-88200370 None:intergenic 35.0%
!! AATCTTCTAAAGTGCCTCTT+TGG - chr6.2:88200351-88200370 MS.gene030325:CDS 35.0%
!! ATTTTCTGGCACAGCAATAA+TGG + chr6.2:88197942-88197961 None:intergenic 35.0%
!! CCCTTATTGATTCTTCATCT+GGG - chr6.2:88197630-88197649 MS.gene030325:intron 35.0%
!! GTTGAGTGATCATGTTGTTT+TGG + chr6.2:88197478-88197497 None:intergenic 35.0%
!! TTTTCTGGCACAGCAATAAT+GGG + chr6.2:88197941-88197960 None:intergenic 35.0%
!!! AAGGCCTTTTTTCACTAGAT+GGG - chr6.2:88199780-88199799 MS.gene030325:CDS 35.0%
!!! ATTTTTTCACACCCTCATGA+TGG - chr6.2:88197599-88197618 MS.gene030325:intron 35.0%
!!! CCCTAACGTTGATTTTATAC+AGG - chr6.2:88198489-88198508 MS.gene030325:intron 35.0%
!!! TTTTTTCACACCCTCATGAT+GGG - chr6.2:88197600-88197619 MS.gene030325:intron 35.0%
AAAGTGGTAGACTGGAACAA+TGG - chr6.2:88199217-88199236 MS.gene030325:intron 40.0%
AACAGCTAGAAACACACACT+TGG + chr6.2:88198191-88198210 None:intergenic 40.0%
AATCAACGTTAGGGAAAGCA+TGG + chr6.2:88198483-88198502 None:intergenic 40.0%
ACATAATCGACCACATGCTT+CGG + chr6.2:88200492-88200511 None:intergenic 40.0%
ACTGTTATATAGGGCCTGTT+TGG - chr6.2:88198684-88198703 MS.gene030325:intron 40.0%
ATGAATACCAATGAGGGTGA+GGG + chr6.2:88197502-88197521 None:intergenic 40.0%
ATGTCAAGAAAAGGGTCAGT+TGG + chr6.2:88197569-88197588 None:intergenic 40.0%
ATTGTCAATGCCGACAACAT+CGG + chr6.2:88198364-88198383 None:intergenic 40.0%
ATTTCGCCAACACTACTAAG+AGG + chr6.2:88197830-88197849 None:intergenic 40.0%
CACAAAATGGGTGTTTCACA+AGG - chr6.2:88197710-88197729 MS.gene030325:CDS 40.0%
CTATTCGTCAATAGGGTTGT+TGG - chr6.2:88199177-88199196 MS.gene030325:intron 40.0%
CTGTTGTTGTAGTGCAACTA+TGG - chr6.2:88199597-88199616 MS.gene030325:intron 40.0%
CTTCATCTTCGGCACTAATA+TGG + chr6.2:88200413-88200432 None:intergenic 40.0%
GAAGCCATGGATTTGACTAA+AGG + chr6.2:88197683-88197702 None:intergenic 40.0%
GAGCAAATATAGTAAAGGGG+AGG + chr6.2:88199479-88199498 None:intergenic 40.0%
GCTCTACAGTCATTGTAATG+AGG - chr6.2:88198269-88198288 MS.gene030325:intron 40.0%
GGCTTCTGCTTTACACAAAA+TGG - chr6.2:88197697-88197716 MS.gene030325:CDS 40.0%
GTTGCACTACAACACTGATA+AGG + chr6.2:88199656-88199675 None:intergenic 40.0%
GTTGTGGATGTGAACATCAA+TGG + chr6.2:88198999-88199018 None:intergenic 40.0%
TACAGGTAGGCTAAGGAATA+AGG + chr6.2:88199868-88199887 None:intergenic 40.0%
TCTTACCCTAGATTTGCATG+AGG - chr6.2:88199383-88199402 MS.gene030325:intron 40.0%
TGTTGGCGAAATTCGTAAAC+AGG - chr6.2:88197838-88197857 MS.gene030325:intron 40.0%
TTCCCCAGTTTCTTCATCTT+CGG + chr6.2:88200424-88200443 None:intergenic 40.0%
TTCTTGACAACAAATGCGAC+TGG + chr6.2:88200450-88200469 None:intergenic 40.0%
TTTGTGTAAAGCAGAAGCCA+TGG + chr6.2:88197696-88197715 None:intergenic 40.0%
! GAGCAGGTTCTTCTTTTGAT+TGG - chr6.2:88200516-88200535 MS.gene030325:CDS 40.0%
! GCATTGACAATGAAGGCAAA+GGG - chr6.2:88198373-88198392 MS.gene030325:intron 40.0%
! TAAGTTGCAGAGTTTGAGAC+AGG - chr6.2:88198408-88198427 MS.gene030325:intron 40.0%
! TTGTATTCATCTGGGACTAC+AGG - chr6.2:88198070-88198089 MS.gene030325:intron 40.0%
! TTTTTCACACCCTCATGATG+GGG - chr6.2:88197601-88197620 MS.gene030325:intron 40.0%
!! AGTCGCATTTGTTGTCAAGA+AGG - chr6.2:88200449-88200468 MS.gene030325:CDS 40.0%
!! ATGAAGGCAAAGGGTGTTAA+TGG - chr6.2:88198382-88198401 MS.gene030325:intron 40.0%
!! GCATTTGTTGTCAAGAAGGT+TGG - chr6.2:88200453-88200472 MS.gene030325:CDS 40.0%
!! GCCCTTATTGATTCTTCATC+TGG - chr6.2:88197629-88197648 MS.gene030325:intron 40.0%
!!! CAAGGCCTTTTTTCACTAGA+TGG - chr6.2:88199779-88199798 MS.gene030325:CDS 40.0%
!!! GGGAGGTAGTTTTGACATAA+AGG + chr6.2:88199462-88199481 None:intergenic 40.0%
AACACAGCTTCTAGATCAGC+AGG + chr6.2:88200227-88200246 None:intergenic 45.0%
AAGATACCAAGGTCTCCGAT+TGG - chr6.2:88200681-88200700 MS.gene030325:CDS 45.0%
AGAGGGCCTTCTATTATGAG+AGG - chr6.2:88199280-88199299 MS.gene030325:intron 45.0%
AGATACCAAGGTCTCCGATT+GGG - chr6.2:88200682-88200701 MS.gene030325:CDS 45.0%
CGTAAACAGGTTCAAGAGTG+TGG - chr6.2:88197851-88197870 MS.gene030325:intron 45.0%
GATGAATACCAATGAGGGTG+AGG + chr6.2:88197503-88197522 None:intergenic 45.0%
GGGTCAGTTGGAAGATTTCT+AGG + chr6.2:88197557-88197576 None:intergenic 45.0%
GTGCCGAAGATGAAGAAACT+GGG - chr6.2:88200418-88200437 MS.gene030325:CDS 45.0%
TCTTCACCGACAAGATACCA+AGG - chr6.2:88200670-88200689 MS.gene030325:CDS 45.0%
TGCCGAAGATGAAGAAACTG+GGG - chr6.2:88200419-88200438 MS.gene030325:CDS 45.0%
TGCTGTGCCAGAAAATGAGA+AGG - chr6.2:88197946-88197965 MS.gene030325:CDS 45.0%
TGGTTTATCGCTCTTTGCTG+CGG - chr6.2:88198231-88198250 MS.gene030325:intron 45.0%
TGTACTTTCTCCGAAGCATG+TGG - chr6.2:88200479-88200498 MS.gene030325:CDS 45.0%
TGTTGTTCCACCGATGTTGT+CGG - chr6.2:88198351-88198370 MS.gene030325:intron 45.0%
TTGCTCCATACAAGAAGGTC+CGG - chr6.2:88200640-88200659 MS.gene030325:CDS 45.0%
TTTCGGAGGATCTTCCCAAT+CGG + chr6.2:88200699-88200718 None:intergenic 45.0%
! AAGAAGGTACAGGTAGGCTA+AGG + chr6.2:88199875-88199894 None:intergenic 45.0%
! AGTGCCGAAGATGAAGAAAC+TGG - chr6.2:88200417-88200436 MS.gene030325:CDS 45.0%
! AGTTTGAGACAGGTCTCTTG+CGG - chr6.2:88198418-88198437 MS.gene030325:intron 45.0%
! CATGAGGTTGATTCAATGGC+TGG - chr6.2:88199399-88199418 MS.gene030325:intron 45.0%
!! AAAGGTTTGAGTTGCTGCAG+TGG + chr6.2:88199444-88199463 None:intergenic 45.0%
!! GGCATTGACAATGAAGGCAA+AGG - chr6.2:88198372-88198391 MS.gene030325:intron 45.0%
!! GTTGTCGGCATTGACAATGA+AGG - chr6.2:88198366-88198385 MS.gene030325:intron 45.0%
!!! AATGGTACTTTTTTGCCCCC+AGG - chr6.2:88199235-88199254 MS.gene030325:intron 45.0%
AATGACTGAGTCGACTGCAG+TGG - chr6.2:88199120-88199139 MS.gene030325:intron 50.0%
ACCTTCCGGACCTTCTTGTA+TGG + chr6.2:88200648-88200667 None:intergenic 50.0%
AGGGCTAAAACCCCATCATG+AGG + chr6.2:88197614-88197633 None:intergenic 50.0%
ATCTTCCCAATCGGAGACCT+TGG + chr6.2:88200690-88200709 None:intergenic 50.0%
ATGACTGAGTCGACTGCAGT+GGG - chr6.2:88199121-88199140 MS.gene030325:intron 50.0%
CAGTGGTGTGACTAACGTTC+AGG + chr6.2:88199427-88199446 None:intergenic 50.0%
CGGAGACCTTGGTATCTTGT+CGG + chr6.2:88200679-88200698 None:intergenic 50.0%
GACAACATCGGTGGAACAAC+AGG + chr6.2:88198352-88198371 None:intergenic 50.0%
GCAGGTTGCTCCATACAAGA+AGG - chr6.2:88200635-88200654 MS.gene030325:CDS 50.0%
GCTGCGGGATTATTGTCGTT+GGG - chr6.2:88198247-88198266 MS.gene030325:intron 50.0%
GGGCTAAAACCCCATCATGA+GGG + chr6.2:88197613-88197632 None:intergenic 50.0%
GGTCGATTATGTTGCTGAGC+AGG - chr6.2:88200500-88200519 MS.gene030325:CDS 50.0%
GGTTTATCGCTCTTTGCTGC+GGG - chr6.2:88198232-88198251 MS.gene030325:intron 50.0%
TCCAAACACGGAGGCAAAAG+TGG - chr6.2:88199201-88199220 MS.gene030325:intron 50.0%
TCCATACAAGAAGGTCCGGA+AGG - chr6.2:88200644-88200663 MS.gene030325:CDS 50.0%
TGCTGCGGGATTATTGTCGT+TGG - chr6.2:88198246-88198265 MS.gene030325:intron 50.0%
TGTCGGTGAAGAACACCTTC+CGG + chr6.2:88200662-88200681 None:intergenic 50.0%
! AGCAATGCCTGAGCTGCTTT+CGG + chr6.2:88200716-88200735 None:intergenic 50.0%
!! ACCACTTTTGCCTCCGTGTT+TGG + chr6.2:88199205-88199224 None:intergenic 50.0%
!! ACGGCTGCATCGACTATTTC+AGG + chr6.2:88200260-88200279 None:intergenic 50.0%
!! AGGGTTGTTGGCTCCAAACA+CGG - chr6.2:88199189-88199208 MS.gene030325:intron 50.0%
AGATCCTCCGAAAGCAGCTC+AGG - chr6.2:88200706-88200725 MS.gene030325:CDS 55.0%
CGGAGGCAAAAGTGGTAGAC+TGG - chr6.2:88199209-88199228 MS.gene030325:intron 55.0%
CTCAACACCCTCACCCTCAT+TGG - chr6.2:88197492-88197511 MS.gene030325:CDS 55.0%
GGGACTACAGGTGTGAGCAA+AGG - chr6.2:88198082-88198101 MS.gene030325:intron 55.0%
GTCAATGCCGACAACATCGG+TGG + chr6.2:88198361-88198380 None:intergenic 55.0%
! AATGCCTGAGCTGCTTTCGG+AGG + chr6.2:88200713-88200732 None:intergenic 55.0%
! GCGGTGAGCTTTGGTTGAGA+GGG - chr6.2:88199263-88199282 MS.gene030325:intron 55.0%
! GGTGCAGCGCTTTTGAGCAT+AGG - chr6.2:88198439-88198458 MS.gene030325:intron 55.0%
! TGCGGTGAGCTTTGGTTGAG+AGG - chr6.2:88199262-88199281 MS.gene030325:intron 55.0%
!! GTTGTTGGCTCCAAACACGG+AGG - chr6.2:88199192-88199211 MS.gene030325:intron 55.0%
AAAGCTCACCGCAGCTGCCT+GGG + chr6.2:88199255-88199274 None:intergenic 60.0%
TCGACTGCAGTGGGAACACG+TGG - chr6.2:88199130-88199149 MS.gene030325:intron 60.0%
! TTTTTGCCCCCAGGCAGCTG+CGG - chr6.2:88199244-88199263 MS.gene030325:intron 60.0%
AAGCTCACCGCAGCTGCCTG+GGG + chr6.2:88199254-88199273 None:intergenic 65.0%
CAAAGCTCACCGCAGCTGCC+TGG + chr6.2:88199256-88199275 None:intergenic 65.0%
CAGGCAGCTGCGGTGAGCTT+TGG - chr6.2:88199254-88199273 MS.gene030325:intron 65.0%
AGCTCACCGCAGCTGCCTGG+GGG + chr6.2:88199253-88199272 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 88197440 88200752 88197440 ID=MS.gene030325
chr6.2 mRNA 88197440 88200752 88197440 ID=MS.gene030325.t1;Parent=MS.gene030325
chr6.2 exon 88199682 88200752 88199682 ID=MS.gene030325.t1.exon1;Parent=MS.gene030325.t1
chr6.2 CDS 88199682 88200752 88199682 ID=cds.MS.gene030325.t1;Parent=MS.gene030325.t1
chr6.2 exon 88198891 88199080 88198891 ID=MS.gene030325.t1.exon2;Parent=MS.gene030325.t1
chr6.2 CDS 88198891 88199080 88198891 ID=cds.MS.gene030325.t1;Parent=MS.gene030325.t1
chr6.2 exon 88198086 88198231 88198086 ID=MS.gene030325.t1.exon3;Parent=MS.gene030325.t1
chr6.2 CDS 88198086 88198231 88198086 ID=cds.MS.gene030325.t1;Parent=MS.gene030325.t1
chr6.2 exon 88197907 88197974 88197907 ID=MS.gene030325.t1.exon4;Parent=MS.gene030325.t1
chr6.2 CDS 88197907 88197974 88197907 ID=cds.MS.gene030325.t1;Parent=MS.gene030325.t1
chr6.2 exon 88197671 88197773 88197671 ID=MS.gene030325.t1.exon5;Parent=MS.gene030325.t1
chr6.2 CDS 88197671 88197773 88197671 ID=cds.MS.gene030325.t1;Parent=MS.gene030325.t1
chr6.2 exon 88197440 88197553 88197440 ID=MS.gene030325.t1.exon6;Parent=MS.gene030325.t1
chr6.2 CDS 88197440 88197553 88197440 ID=cds.MS.gene030325.t1;Parent=MS.gene030325.t1
Gene Sequence

>MS.gene030325

ATGGCTTCTGCTGCAAATTTAAACTGTGAAATCACCCAAAACAACATGATCACTCAACACCCTCACCCTCATTGGTATTCATCAAAAACTGGAATTTACAACAGTATACACACTCCTAGAAATCTTCCAACTGACCCTTTTCTTGACATTGTTTCCTTCATTTTTTCACACCCTCATGATGGGGTTTTAGCCCTTATTGATTCTTCATCTGGGTCTTCCATTTCTTACTCAAAGTTGTTACCTTTAGTCAAATCCATGGCTTCTGCTTTACACAAAATGGGTGTTTCACAAGGTGATGTTGTTTTGCTTCTACTTCCAAATTCAATTTATTACCCTGTTATACTATTGGCTGTTCTGTATTTAGGTGCTGTTTTTACACCATTGAATCCTCTTAGTAGTGTTGGCGAAATTCGTAAACAGGTTCAAGAGTGTGGTGTGAGTTTTGCTTTTACTATACCTGAAAATGTCAAGAAACTAGAGCTATTAGGTGGCATTAATCCCATTATTGCTGTGCCAGAAAATGAGAAGGATTTGAGGAATGAGTGTTTCTCAAGTTTTTTTAACCTTCTTTATGGTAAATTTGATTTGCCTCAAAGGGTAGTTATTAAACAGGAAGATACTGCTGGTATATTGTATTCATCTGGGACTACAGGTGTGAGCAAAGGAGTTGTTTTAACTCATAGAAATCTTATTTCTATGGTTGAGCTTTTTGTGAGATTTGAAGCTTCTCAATATGATTACTCTTGTTCCAAGTGTGTGTTTCTAGCTGTTTTGCCAATGTTTCATGTGTATGGTTTATCGCTCTTTGCTGCGGGATTATTGTCGTTGGGCTCTACAGTCATTGTAATGAGGAAATTTGACATTGATGAGGTTATTAGGGTGATTGACAAATATAATGTTACACACTTTCCTGTTGTTCCACCGATGTTGTCGGCATTGACAATGAAGGCAAAGGGTGTTAATGGAAGTAAGTTGCAGAGTTTGAGACAGGTCTCTTGCGGTGCAGCGCTTTTGAGCATAGGAGTTATTAGTAGTTTTGTCCATGCTTTCCCTAACGTTGATTTTATACAGGTAAAGAACCTTTGAATAGAATAGTCTTAATTAGAGAATGCAAGTAATGCTCCATCGTTTATATGATTGCTTGAGTTAATAGTTATTTTTCTTTGTTATTGTTGGTAGTAGCTAAATGCTGATTATTTCATTTAATTATGAGAGAACGTGTGAGCTTTAAAAATTTAATAGCAACTGTTATATAGGGCCTGTTTGGCTAAACAACTTATTTGGTGCTTATAGCATAAGCGCTTATCATAATAAGCACTTCTATATACACATACATAAGCTATTTCTATAGCAAAAAATAAAATAAAGTAAAATTGTTTTTGTATATGTTAAAAGTCTTTTTCATAAGCTATCTTGGATAACTTATAAAAATAAGCTGAACAGAACTAAGGAAAAATGTCAAAAAAGCTATTTTCATAAGTTCTCCCAAACAGTCTCGCAAAACTTATGCCAGTAGACAAGCTCAAACAATTTGTTTTCATAATACTCTTTTTGTGCCATTGATGTTCACATCCACAACAAATTCTAGATCATAAAAGTTGAACTTTATTTGTTTATGATAATAATGTTGTTTTCCTAGATTATTAATAGCTGTAAGATTGTTTCTTTTCAGGGTTATGGAATGACTGAGTCGACTGCAGTGGGAACACGTGGCTTCAATACTGAAAAGATTCACAACTATTCGTCAATAGGGTTGTTGGCTCCAAACACGGAGGCAAAAGTGGTAGACTGGAACAATGGTACTTTTTTGCCCCCAGGCAGCTGCGGTGAGCTTTGGTTGAGAGGGCCTTCTATTATGAGAGGTAAAAAATCTGTATTTTTCTGAATAGATATTAAAAAGTAAAACACGGGAGTAATATAAAGGTCTTGTCTATAGTAGCCAATCTTACCCTAGATTTGCATGAGGTTGATTCAATGGCTGGAACCTGAACGTTAGTCACACCACTGCAGCAACTCAAACCTTTATGTCAAAACTACCTCCCCTTTACTATATTTGCTCTATATTTGTTATCGCTTAACTAAAGGCCTGTGTCCTTGAGAAATATCATGTTAGTTTAGTAGACATTTTATTCTTCTAGAGTGATGCTATACACTCATCTGTTGTTGTAGTGCAACTATGGACGCGAATTCAGTTGAAAGATACATATACATCTCCTTATCAGTGTTGTAGTGCAACTATTGACGCGAATTCAGTTGAAAGTTACATATACATCTCCTTATCAGTAAAATGTTAATGAAAGTTTTCTTTCAATTTAGTTTAGATAGCGAACAACAACAATCAAGGCCTTTTTTCACTAGATGGGAAAACTGAAGTAAATTTCTCTTGGAGTCAACTGTTTCATTGCTATAATAACTTTTGTTTCTGCCTTATTCCTTAGCCTACCTGTACCTTCTTTTTTCTTGTCTTGCTGCTATTTGTCCAATTAAAATTGTTAATCAAACTCTTTCCTTTAATTCTCAGGATACTTGAATAATGAGGAAGCTACTATATCAACAATTGATAAAGATGGTTGGTTACATACAGGAGATATTGTTTATTTTGATCAGGATGGATATTTACATTTGGCTGATCGCTTGAAAGACATTATCAAATACAAGGGATTTCAGGTATCTACTTTTAGGTTTATTAAATTTTATAAAGAAAAGTGATGATAGATTAACGAGAAATGCAGAATTTTACTGAAACAAACACTTTGTATGACACATGATCTGTTACAGATTGCTCCTGCTGATCTAGAAGCTGTGTTGATTTTGCATCCTGAAATAGTCGATGCAGCCGTTACAGCGTAAGTATATGAACACTTCATTTATGTCTTCGCTTCTACAATCTATAGAAAATGCAGTCATTCCTAATCTTCTAAAGTGCCTCTTTGGACATTTGATTATAACCTCAAATGTTACATCATTTTTCCATATTAGTGCCGAAGATGAAGAAACTGGGGAAATTCCAGTCGCATTTGTTGTCAAGAAGGTTGGAAGTGTACTTTCTCCGAAGCATGTGGTCGATTATGTTGCTGAGCAGGTTCTTCTTTTGATTGGACAGACATGATTTTTATGATATTTGAATTGATATTACTACTTGAATCAATGTCATGCTTTTCACATTTTAACCCTTTATTTTATTTTTTTCTTCTGGCAGGTTGCTCCATACAAGAAGGTCCGGAAGGTGTTCTTCACCGACAAGATACCAAGGTCTCCGATTGGGAAGATCCTCCGAAAGCAGCTCAGGCATTGCTTGACTTCTAAACTCTAG
Protein sequence

>MS.gene030325.t1

MASAANLNCEITQNNMITQHPHPHWYSSKTGIYNSIHTPRNLPTDPFLDIVSFIFSHPHDGVLALIDSSSGSSISYSKLLPLVKSMASALHKMGVSQGDVVLLLLPNSIYYPVILLAVLYLGAVFTPLNPLSSVGEIRKQVQECGVSFAFTIPENVKKLELLGGINPIIAVPENEKDLRNECFSSFFNLLYGKFDLPQRVVIKQEDTAGILYSSGTTGVSKGVVLTHRNLISMVELFVRFEASQYDYSCSKCVFLAVLPMFHVYGLSLFAAGLLSLGSTVIVMRKFDIDEVIRVIDKYNVTHFPVVPPMLSALTMKAKGVNGSKLQSLRQVSCGAALLSIGVISSFVHAFPNVDFIQGYGMTESTAVGTRGFNTEKIHNYSSIGLLAPNTEAKVVDWNNGTFLPPGSCGELWLRGPSIMRGYLNNEEATISTIDKDGWLHTGDIVYFDQDGYLHLADRLKDIIKYKGFQIAPADLEAVLILHPEIVDAAVTAAEDEETGEIPVAFVVKKVGSVLSPKHVVDYVAEQVAPYKKVRKVFFTDKIPRSPIGKILRKQLRHCLTSKL