AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031746

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031746.t1 MTR_4g077900 97.908 478 10 0 86 563 1 478 0.0 957
MS.gene031746.t1 MTR_5g027480 79.821 560 107 2 4 563 2 555 0.0 912
MS.gene031746.t1 MTR_5g027500 79.545 572 100 3 4 563 2 568 0.0 901
MS.gene031746.t1 MTR_1g026310 45.138 545 288 5 26 563 20 560 1.98e-160 469
MS.gene031746.t1 MTR_8g039720 44.102 551 287 7 26 563 17 559 7.34e-155 455
MS.gene031746.t1 MTR_1g090737 44.604 556 278 12 26 563 27 570 1.13e-140 419
MS.gene031746.t1 MTR_4g057730 41.651 533 295 8 31 555 10 534 1.87e-133 400
MS.gene031746.t1 MTR_1g063120 40.619 549 301 9 24 555 4 544 4.26e-130 391
MS.gene031746.t1 MTR_3g088880 41.532 496 271 6 64 555 68 548 9.04e-129 388
MS.gene031746.t1 MTR_3g088870 42.771 498 265 9 64 555 67 550 1.08e-128 388
MS.gene031746.t1 MTR_2g095510 39.372 541 309 7 31 563 10 539 4.68e-128 385
MS.gene031746.t1 MTR_1g063110 39.963 543 309 7 24 555 4 540 4.81e-125 378
MS.gene031746.t1 MTR_2g105570 39.739 536 305 10 36 561 20 547 2.60e-113 348
MS.gene031746.t1 MTR_4g057730 41.880 468 256 8 31 490 10 469 2.14e-111 340
MS.gene031746.t1 MTR_1g073180 39.080 522 279 11 48 555 30 526 2.28e-109 337
MS.gene031746.t1 MTR_4g128337 38.189 508 298 10 64 563 49 548 1.05e-103 323
MS.gene031746.t1 MTR_4g005750 38.418 531 313 9 36 561 19 540 2.15e-102 320
MS.gene031746.t1 MTR_4g057730 40.576 451 248 8 31 471 10 450 1.60e-101 314
MS.gene031746.t1 MTR_5g007640 38.492 504 299 7 64 561 87 585 1.66e-100 316
MS.gene031746.t1 MTR_1g073180 39.228 492 264 11 60 542 69 534 6.41e-100 313
MS.gene031746.t1 MTR_3g460770 34.004 547 339 8 26 563 8 541 3.85e-98 309
MS.gene031746.t1 MTR_2g095510 38.053 452 261 7 31 474 10 450 1.83e-95 299
MS.gene031746.t1 MTR_1g026470 39.637 386 222 5 26 404 20 401 4.57e-85 270
MS.gene031746.t1 MTR_1g026430 39.637 386 222 5 26 404 20 401 1.15e-84 269
MS.gene031746.t1 MTR_3g035130 31.474 502 294 16 74 553 32 505 7.21e-55 194
MS.gene031746.t1 MTR_1569s0010 27.992 518 327 12 74 556 22 528 2.09e-46 171
MS.gene031746.t1 MTR_5g076240 28.820 517 319 14 75 557 41 542 5.95e-45 167
MS.gene031746.t1 MTR_3g035130 31.429 455 264 16 74 507 32 459 9.66e-43 159
MS.gene031746.t1 MTR_3g064420 27.290 535 318 16 70 556 36 547 1.86e-40 155
MS.gene031746.t1 MTR_5g076220 28.000 525 324 16 70 557 36 543 2.33e-39 151
MS.gene031746.t1 MTR_0161s0050 29.491 373 227 9 204 556 188 544 3.72e-39 151
MS.gene031746.t1 MTR_0803s0020 28.458 506 296 16 76 549 263 734 1.18e-38 152
MS.gene031746.t1 MTR_4g011790 28.389 391 247 10 196 562 217 598 1.35e-38 150
MS.gene031746.t1 MTR_4g013210 29.455 404 242 13 192 562 52 445 7.19e-38 145
MS.gene031746.t1 MTR_4g011790 28.496 379 238 10 196 550 217 586 8.57e-37 145
MS.gene031746.t1 MTR_4g011790 27.750 400 247 12 196 562 217 607 2.20e-36 144
MS.gene031746.t1 MTR_8g074040 28.433 517 313 19 86 563 94 592 9.47e-36 142
MS.gene031746.t1 MTR_5g076200 26.718 524 330 16 70 556 36 542 2.35e-34 137
MS.gene031746.t1 MTR_5g008820 27.326 516 316 18 75 556 41 531 5.22e-33 133
MS.gene031746.t1 MTR_2g030240 26.015 542 307 15 77 556 31 540 5.89e-33 133
MS.gene031746.t1 MTR_2g044180 30.707 368 216 11 212 556 110 461 7.51e-33 131
MS.gene031746.t1 MTR_2g044180 30.707 368 216 11 212 556 201 552 1.60e-32 132
MS.gene031746.t1 MTR_8g074040 29.427 384 228 14 206 563 108 474 7.35e-32 129
MS.gene031746.t1 MTR_5g023730 25.532 517 337 16 75 561 44 542 1.80e-31 129
MS.gene031746.t1 MTR_5g008800 26.810 511 329 14 75 557 41 534 1.85e-31 129
MS.gene031746.t1 MTR_4g013210 28.954 373 222 12 192 531 204 566 1.28e-30 126
MS.gene031746.t1 MTR_8g074120 28.191 376 223 12 206 557 137 489 5.53e-30 124
MS.gene031746.t1 MTR_4g121600 25.143 525 331 15 75 556 47 552 5.63e-30 124
MS.gene031746.t1 MTR_4g013210 27.157 394 223 11 192 562 52 404 1.75e-29 120
MS.gene031746.t1 MTR_4g013210 26.650 394 225 10 192 562 204 556 6.16e-29 121
MS.gene031746.t1 MTR_2g030240 26.823 384 230 9 210 556 77 446 9.32e-29 119
MS.gene031746.t1 MTR_1g026350 41.791 134 78 0 26 159 20 153 1.16e-28 112
MS.gene031746.t1 MTR_3g087980 26.749 486 268 18 74 483 106 579 1.20e-26 115
MS.gene031746.t1 MTR_1g104810 24.906 530 306 15 27 480 55 568 2.98e-26 114
MS.gene031746.t1 MTR_1g104810 28.125 384 210 12 146 480 135 501 1.32e-24 108
MS.gene031746.t1 MTR_7g109830 26.255 518 306 18 43 490 36 547 2.74e-24 107
MS.gene031746.t1 MTR_1g030290 29.912 341 171 14 206 485 250 583 1.34e-23 105
MS.gene031746.t1 MTR_7g084850 23.697 595 394 18 8 563 151 724 2.79e-23 104
MS.gene031746.t1 MTR_5g009350 25.047 535 312 19 28 485 22 544 1.06e-22 102
MS.gene031746.t1 MTR_5g009350 25.047 535 312 19 28 485 22 544 1.45e-22 102
MS.gene031746.t1 MTR_5g009350 25.047 535 312 19 28 485 22 544 1.50e-22 102
MS.gene031746.t1 MTR_5g009350 25.047 535 312 19 28 485 22 544 1.75e-22 101
MS.gene031746.t1 MTR_1g086600 24.517 518 317 14 43 490 58 571 4.59e-22 100
MS.gene031746.t1 MTR_3g084450 25.688 545 295 18 63 503 94 632 5.65e-22 100
MS.gene031746.t1 MTR_5g009360 24.798 496 297 16 56 485 59 544 1.35e-21 99.4
MS.gene031746.t1 MTR_8g099420 25.100 502 300 17 45 480 49 540 6.62e-21 97.1
MS.gene031746.t1 MTR_4g057755 52.778 72 34 0 419 490 46 117 9.18e-21 88.6
MS.gene031746.t1 MTR_5g009360 28.024 339 189 11 194 485 88 418 1.05e-19 93.2
MS.gene031746.t1 MTR_3g082660 25.971 489 261 21 75 479 138 609 9.55e-19 90.5
MS.gene031746.t1 MTR_1054s0020 23.307 502 339 13 71 554 294 767 1.17e-18 90.5
MS.gene031746.t1 MTR_8g083170 31.356 236 130 8 311 533 448 664 7.88e-18 87.8
MS.gene031746.t1 MTR_4g013210 26.821 302 167 9 192 480 204 464 7.04e-17 84.0
MS.gene031746.t1 MTR_3g084450 34.356 163 92 4 353 503 265 424 3.40e-16 82.0
MS.gene031746.t1 MTR_3g084470 30.380 237 128 7 353 555 469 702 4.89e-16 82.0
MS.gene031746.t1 MTR_3g088890 50.000 64 32 0 465 528 10 73 2.03e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031746.t1 AT4G19010 60.000 555 213 5 15 563 11 562 0.0 667
MS.gene031746.t1 AT1G20510 44.068 531 292 2 26 555 10 536 2.22e-163 476
MS.gene031746.t1 AT1G20480 41.894 549 298 7 26 563 27 565 5.90e-150 442
MS.gene031746.t1 AT1G20500 42.210 552 297 7 21 563 12 550 1.23e-148 439
MS.gene031746.t1 AT5G38120 40.951 547 294 9 21 555 13 542 3.26e-136 407
MS.gene031746.t1 AT1G20510 42.918 466 261 2 26 490 10 471 1.06e-135 403
MS.gene031746.t1 AT4G05160 40.183 545 308 6 29 563 8 544 9.36e-135 403
MS.gene031746.t1 AT5G63380 39.450 545 304 7 26 555 17 550 2.49e-133 400
MS.gene031746.t1 AT1G20510 41.480 446 256 2 26 470 10 451 4.13e-123 370
MS.gene031746.t1 AT5G38120 39.692 519 284 9 21 527 13 514 2.14e-121 368
MS.gene031746.t1 AT3G21240 39.261 568 316 12 11 563 1 554 1.13e-115 354
MS.gene031746.t1 AT5G38120 39.834 482 261 9 21 490 13 477 3.79e-111 340
MS.gene031746.t1 AT5G38120 39.834 482 261 9 21 490 13 477 3.79e-111 340
MS.gene031746.t1 AT1G51680 41.292 511 284 6 64 563 56 561 1.86e-109 338
MS.gene031746.t1 AT1G65060 39.051 548 302 11 21 556 30 557 1.71e-105 328
MS.gene031746.t1 AT3G21230 38.078 562 330 8 10 561 13 566 2.50e-104 325
MS.gene031746.t1 AT1G20490 39.394 429 240 8 21 440 12 429 2.03e-95 298
MS.gene031746.t1 AT1G20490 39.394 429 240 8 21 440 12 429 2.03e-95 298
MS.gene031746.t1 AT1G51680 40.421 475 267 6 64 527 56 525 9.39e-95 300
MS.gene031746.t1 AT3G21230 37.689 528 311 8 10 527 13 532 8.76e-93 295
MS.gene031746.t1 AT1G62940 37.103 504 294 9 63 556 43 533 2.70e-92 293
MS.gene031746.t1 AT3G21240 38.028 497 279 12 11 492 1 483 7.93e-90 285
MS.gene031746.t1 AT3G21240 38.028 497 279 12 11 492 1 483 7.93e-90 285
MS.gene031746.t1 AT1G51680 39.773 440 249 6 64 492 56 490 4.46e-84 270
MS.gene031746.t1 AT3G21230 37.120 493 292 8 10 492 13 497 1.40e-82 266
MS.gene031746.t1 AT1G65060 36.638 464 262 11 21 472 30 473 1.27e-73 243
MS.gene031746.t1 AT3G48990 28.866 485 331 9 73 553 30 504 2.98e-50 181
MS.gene031746.t1 AT3G21230 32.464 422 267 8 10 421 13 426 4.83e-46 168
MS.gene031746.t1 AT5G16370 29.980 507 300 18 86 557 53 539 1.18e-45 169
MS.gene031746.t1 AT1G77240 29.791 527 306 19 73 561 43 543 1.49e-44 166
MS.gene031746.t1 AT1G21540 27.667 553 339 19 47 561 19 548 5.02e-43 162
MS.gene031746.t1 AT5G16340 29.883 512 292 18 86 556 53 538 1.56e-42 160
MS.gene031746.t1 AT1G21530 28.516 519 318 19 75 557 42 543 1.15e-41 158
MS.gene031746.t1 AT1G65890 26.515 528 329 17 72 556 38 549 2.84e-41 157
MS.gene031746.t1 AT1G21530 28.433 517 319 19 75 557 42 541 2.15e-40 154
MS.gene031746.t1 AT1G65880 25.940 532 328 16 72 556 38 550 2.59e-39 152
MS.gene031746.t1 AT1G20560 29.026 503 308 15 86 556 54 539 5.71e-38 147
MS.gene031746.t1 AT1G75960 28.968 504 309 16 86 557 53 539 1.75e-37 146
MS.gene031746.t1 AT1G66120 27.413 518 330 15 72 556 38 542 3.70e-37 145
MS.gene031746.t1 AT1G30520 27.119 531 306 17 83 557 42 547 5.48e-36 142
MS.gene031746.t1 AT1G30520 27.119 531 306 17 83 557 42 547 5.48e-36 142
MS.gene031746.t1 AT1G20560 30.548 383 231 10 192 556 96 461 1.08e-33 134
MS.gene031746.t1 AT3G16910 29.949 394 231 10 189 556 181 555 1.14e-33 135
MS.gene031746.t1 AT3G16170 27.835 388 236 12 205 562 234 607 2.18e-33 135
MS.gene031746.t1 AT2G17650 26.336 524 305 18 86 556 99 594 3.53e-33 134
MS.gene031746.t1 AT1G76290 25.806 496 327 16 86 556 52 531 6.73e-30 124
MS.gene031746.t1 AT1G64400 25.144 521 307 16 32 478 27 538 1.35e-26 115
MS.gene031746.t1 AT1G30520 26.622 447 258 16 83 487 42 460 1.73e-26 113
MS.gene031746.t1 AT1G30520 26.622 447 258 16 83 487 42 460 1.73e-26 113
MS.gene031746.t1 AT5G27600 27.619 525 294 17 27 478 58 569 2.77e-26 114
MS.gene031746.t1 AT5G36880 24.906 530 354 15 72 563 146 669 9.24e-26 112
MS.gene031746.t1 AT5G36880 24.906 530 354 15 72 563 146 669 9.24e-26 112
MS.gene031746.t1 AT5G36880 24.906 530 354 15 72 563 146 669 9.24e-26 112
MS.gene031746.t1 AT5G36880 24.906 530 354 15 72 563 146 669 9.24e-26 112
MS.gene031746.t1 AT5G36880 24.906 530 354 15 72 563 196 719 1.20e-25 112
MS.gene031746.t1 AT4G11030 23.722 489 298 15 57 478 58 538 9.66e-25 109
MS.gene031746.t1 AT1G68270 36.076 158 90 2 409 556 363 519 2.29e-23 104
MS.gene031746.t1 AT1G49430 26.004 523 304 21 41 490 39 551 4.79e-23 103
MS.gene031746.t1 AT3G05970 30.699 329 170 11 197 478 252 569 8.76e-23 103
MS.gene031746.t1 AT4G23850 23.800 521 314 19 32 478 27 538 4.24e-22 100
MS.gene031746.t1 AT1G77590 28.080 349 181 14 206 492 100 440 1.35e-21 99.0
MS.gene031746.t1 AT1G77590 28.080 349 181 14 206 492 246 586 2.15e-21 99.0
MS.gene031746.t1 AT2G04350 24.380 484 275 19 75 479 131 602 4.32e-16 82.0
MS.gene031746.t1 AT2G04350 24.380 484 275 19 75 479 131 602 4.32e-16 82.0
MS.gene031746.t1 AT3G23790 32.919 161 93 4 353 501 474 631 6.36e-15 78.6
MS.gene031746.t1 AT2G47240 32.903 155 98 5 328 479 326 477 1.74e-11 67.4
MS.gene031746.t1 AT2G47240 32.903 155 98 5 328 479 385 536 2.08e-11 67.4
MS.gene031746.t1 AT2G47240 32.903 155 98 5 328 479 385 536 2.08e-11 67.4
MS.gene031746.t1 AT2G47240 32.903 155 98 5 328 479 385 536 2.08e-11 67.4
MS.gene031746.t1 AT4G14070 27.899 276 167 8 305 552 440 711 4.99e-11 66.2

Find 130 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTGCTTTACACAAAA+TGG 0.138296 4.3:-31048638 MS.gene031746:CDS
GCTGGTATATTGTATTCTTC+TGG 0.174054 4.3:-31048274 MS.gene031746:CDS
ACAGGATAGTAAATTGAATT+TGG 0.180607 4.3:+31048580 None:intergenic
CTCTTGGCTGTTCTGTATTT+AGG 0.185255 4.3:-31048553 MS.gene031746:CDS
GCCCTTATTGATTCTTCATC+TGG 0.217618 4.3:-31048706 MS.gene031746:CDS
TATCAAATACAAGGGATTTC+AGG 0.243292 4.3:-31046389 MS.gene031746:intron
GTTGAGTGATCATGTTGTTT+TGG 0.245364 4.3:+31048860 None:intergenic
TTGACATTGACGAGGTTATC+AGG 0.253484 4.3:-31048039 MS.gene031746:CDS
ACGGCTGCATCGACTATTTC+AGG 0.272026 4.3:+31046217 None:intergenic
AGTGCCGAAGATGAAGAAAT+TGG 0.290280 4.3:-31046057 MS.gene031746:CDS
TCATAGAAATCTAATTTCTA+TGG 0.293867 4.3:-31048218 MS.gene031746:CDS
TTTCACAACTATTCGTCAAT+AGG 0.295826 4.3:-31047204 MS.gene031746:CDS
GGGTCAGTTGGAAGGTTTCT+AGG 0.297530 4.3:+31048781 None:intergenic
ATGAAGGCAAAGGGTGTTAA+TGG 0.303488 4.3:-31047953 MS.gene031746:CDS
CAGGCAGCTGCGGTGAGCTT+TGG 0.328932 4.3:-31047119 MS.gene031746:CDS
CAAAGCTCACCGCAGCTGCC+TGG 0.332403 4.3:+31047120 None:intergenic
TTGCCAATGTTTCATGTGTA+TGG 0.342668 4.3:-31048124 MS.gene031746:CDS
CTGGTATATTGTATTCTTCT+GGG 0.346881 4.3:-31048273 MS.gene031746:CDS
ACCCAGATGAAGAATCAATA+AGG 0.350199 4.3:+31048704 None:intergenic
AACAGCTAGAAACACATTCT+TGG 0.351291 4.3:+31048147 None:intergenic
AGCAGTGCCTGAGTTGCTTT+CGG 0.356544 4.3:+31045759 None:intergenic
TTCCCCAATTTCTTCATCTT+CGG 0.365786 4.3:+31046053 None:intergenic
TTCTTACCTCTCATAATAGA+AGG 0.374837 4.3:+31047087 None:intergenic
TTCACAACTATTCGTCAATA+GGG 0.376437 4.3:-31047203 MS.gene031746:CDS
ACCTGTATAAAATCAACGTT+AGG 0.379226 4.3:+31047845 None:intergenic
TGTTGGTGAAATTCGTAAAC+AGG 0.387052 4.3:-31048497 MS.gene031746:CDS
AAAGCTCACCGCAGCTGCCT+GGG 0.391998 4.3:+31047121 None:intergenic
TTACTATCCTGTTATACTCT+TGG 0.392695 4.3:-31048569 MS.gene031746:CDS
AGAACAGCCAAGAGTATAAC+AGG 0.393213 4.3:+31048562 None:intergenic
GTGCCGAAGATGAAGAAATT+GGG 0.397388 4.3:-31046056 MS.gene031746:CDS
ACCTTCCGGACCTTCTTGTA+TGG 0.403187 4.3:+31045827 None:intergenic
TGCTGCGGGATTATTGTCGT+TGG 0.410245 4.3:-31048089 MS.gene031746:CDS
TGTTGTTCCACCGATGTTGT+CGG 0.411176 4.3:-31047984 MS.gene031746:CDS
AAGGAAACAATGTCAAGAAA+AGG 0.413052 4.3:+31048760 None:intergenic
CCCTTATTGATTCTTCATCT+GGG 0.414327 4.3:-31048705 MS.gene031746:CDS
TGCTTGAGTTCTAAACTCTA+AGG 0.416696 4.3:-31045740 None:intergenic
TCAGGATGGATATTTACATT+TGG 0.418131 4.3:-31046431 MS.gene031746:CDS
GTCAAGAAACTAGAGCTATT+AGG 0.418468 4.3:-31048430 MS.gene031746:CDS
TCAAAGGGTAGTTATTAAAC+AGG 0.419883 4.3:-31048305 MS.gene031746:CDS
CTATTCGTCAATAGGGTTGT+TGG 0.424322 4.3:-31047196 MS.gene031746:CDS
GACAACATCGGTGGAACAAC+AGG 0.427365 4.3:+31047986 None:intergenic
TGTTTAATAACTACCCTTTG+AGG 0.430394 4.3:+31048307 None:intergenic
GGTTTATCGCTCTTTGCTGC+GGG 0.430597 4.3:-31048103 MS.gene031746:CDS
AAACAACTTTGAGTAAGAAA+TGG 0.436153 4.3:+31048678 None:intergenic
CAGAAAATGAGAAGGATTTG+AGG 0.445513 4.3:-31048381 MS.gene031746:CDS
CTCAACACCCTCACCCTCAT+TGG 0.448612 4.3:-31048843 MS.gene031746:CDS
TGCGGTGAGCTTTGGTTGAG+AGG 0.454034 4.3:-31047111 MS.gene031746:CDS
GCTTCTGCTTTACACAAAAT+GGG 0.455667 4.3:-31048637 MS.gene031746:CDS
GCTGCGGGATTATTGTCGTT+GGG 0.458574 4.3:-31048088 MS.gene031746:CDS
TTGTATTCTTCTGGGACTAC+AGG 0.459563 4.3:-31048265 MS.gene031746:CDS
TGTCGGTGATGAACACCTTC+CGG 0.460828 4.3:+31045813 None:intergenic
CCCAGATGAAGAATCAATAA+GGG 0.467167 4.3:+31048705 None:intergenic
AATCAAATTTACCATAAAGA+AGG 0.470429 4.3:+31048332 None:intergenic
TAAATTTGATTTGCCTCAAA+GGG 0.485047 4.3:-31048320 MS.gene031746:CDS
GGTGCAGCGCCTTTGAGCAT+AGG 0.486009 4.3:-31047896 MS.gene031746:CDS
GTAAATTTGATTTGCCTCAA+AGG 0.487083 4.3:-31048321 MS.gene031746:CDS
GCATTTGTTGTCAAGAAGGT+TGG 0.487321 4.3:-31046021 MS.gene031746:CDS
ACATAATCGACCACATGCTT+CGG 0.487322 4.3:+31045985 None:intergenic
ATTTCACCAACACTACTAAG+AGG 0.489624 4.3:+31048508 None:intergenic
CGGAGACCTTGGTATCTTGT+CGG 0.489928 4.3:+31045796 None:intergenic
AGTGGCATTTGTTGTCAAGA+AGG 0.496940 4.3:-31046025 MS.gene031746:CDS
TTGAGCATAGGAGTTATTAG+TGG 0.499006 4.3:-31047884 MS.gene031746:CDS
AACACAGCTTCTAGATCAGC+AGG 0.500308 4.3:+31046250 None:intergenic
CACAAAATGGGTGTTTCACA+AGG 0.502968 4.3:-31048625 MS.gene031746:CDS
TTGAATCCTCTTAGTAGTGT+TGG 0.505325 4.3:-31048514 MS.gene031746:CDS
ATCTTCCCAGTCGGAGACCT+TGG 0.507080 4.3:+31045785 None:intergenic
TGGTTTATCGCTCTTTGCTG+CGG 0.507976 4.3:-31048104 MS.gene031746:CDS
TTCTTGACAACAAATGCCAC+TGG 0.510888 4.3:+31046027 None:intergenic
TAAGTTGCAGAGTTTGAGAC+AGG 0.511530 4.3:-31047927 MS.gene031746:CDS
TTGCTCCATACAAGAAGGTC+CGG 0.515213 4.3:-31045832 MS.gene031746:CDS
CGGAGGCAAAAGTGGTAGAC+TGG 0.515561 4.3:-31047164 MS.gene031746:CDS
AAAGATGGTTGGCTACATAC+AGG 0.515947 4.3:-31046472 MS.gene031746:CDS
GCGGTGAGCTTTGGTTGAGA+GGG 0.517348 4.3:-31047110 MS.gene031746:CDS
AGGAAACAATGTCAAGAAAA+GGG 0.521407 4.3:+31048761 None:intergenic
AAGCTCACCGCAGCTGCCTG+GGG 0.523366 4.3:+31047122 None:intergenic
ATGTCAAGAAAAGGGTCAGT+TGG 0.533509 4.3:+31048769 None:intergenic
AAACCATACACATGAAACAT+TGG 0.533547 4.3:+31048121 None:intergenic
TCCAAACACGGAGGCAAAAG+TGG 0.534472 4.3:-31047172 MS.gene031746:CDS
ATATCAACAATTGATAAAGA+TGG 0.537763 4.3:-31046487 MS.gene031746:CDS
AGATCCTCCGAAAGCAACTC+AGG 0.540928 4.3:-31045766 MS.gene031746:CDS
AAAGTGGTAGACTGGAACAA+TGG 0.541163 4.3:-31047156 MS.gene031746:CDS
CTCAGGATACTTGAATAATG+AGG 0.541417 4.3:-31046518 MS.gene031746:intron
TTCATATACTTACGCTGTAA+CGG 0.543086 4.3:+31046198 None:intergenic
GAAGCCATGGATTTGACTAA+AGG 0.543817 4.3:+31048655 None:intergenic
CAAGAAAAGGGTCAGTTGGA+AGG 0.544290 4.3:+31048773 None:intergenic
AATCAACGTTAGGGAAAGCA+TGG 0.550452 4.3:+31047855 None:intergenic
GAAAGACATTATCAAATACA+AGG 0.550958 4.3:-31046398 MS.gene031746:CDS
GGTCGATTATGTTGCTGAGC+AGG 0.550976 4.3:-31045974 MS.gene031746:intron
GGGACTACAGGTGTGAGTAA+AGG 0.553349 4.3:-31048253 MS.gene031746:CDS
GTTTCCTTTAGTCAAATCCA+TGG 0.554922 4.3:-31048659 MS.gene031746:CDS
TTTGTGTAAAGCAGAAGCCA+TGG 0.560434 4.3:+31048642 None:intergenic
CCTGTATAAAATCAACGTTA+GGG 0.562718 4.3:+31047846 None:intergenic
TGACATTGACGAGGTTATCA+GGG 0.564262 4.3:-31048038 MS.gene031746:CDS
ACACTACTAAGAGGATTCAA+TGG 0.565007 4.3:+31048517 None:intergenic
CAACAATTGATAAAGATGGT+TGG 0.569594 4.3:-31046483 MS.gene031746:CDS
GGCATTGACAATGAAGGCAA+AGG 0.569984 4.3:-31047963 MS.gene031746:CDS
GCATTGACAATGAAGGCAAA+GGG 0.570042 4.3:-31047962 MS.gene031746:CDS
ATTTGATAATGTCTTTCAAG+CGG 0.579224 4.3:+31046404 None:intergenic
ATTGTCAATGCCGACAACAT+CGG 0.579863 4.3:+31047974 None:intergenic
TGCTGTGCCAGAAAATGAGA+AGG 0.586565 4.3:-31048389 MS.gene031746:CDS
AGTTTGAGACAGGTCTCTTG+CGG 0.592614 4.3:-31047917 MS.gene031746:CDS
GATGAATACCAATGAGGGTG+AGG 0.592922 4.3:+31048835 None:intergenic
GTTGTCGGCATTGACAATGA+AGG 0.595731 4.3:-31047969 MS.gene031746:CDS
GATCTACTGTCATTGTAATG+AGG 0.600626 4.3:-31048066 MS.gene031746:CDS
TGCCGAAGATGAAGAAATTG+GGG 0.602629 4.3:-31046055 MS.gene031746:CDS
AAGATACCAAGGTCTCCGAC+TGG 0.604082 4.3:-31045791 MS.gene031746:CDS
CTAATAACTCCTATGCTCAA+AGG 0.604731 4.3:+31047887 None:intergenic
ATTAAACAGGAAGATACTGC+TGG 0.608838 4.3:-31048292 MS.gene031746:CDS
CGTAAACAGGTTCAAGAGTG+TGG 0.611754 4.3:-31048484 MS.gene031746:CDS
AGGGTTGTTGGCTCCAAACA+CGG 0.614077 4.3:-31047184 MS.gene031746:CDS
AAAGACATTATCAAATACAA+GGG 0.615550 4.3:-31046397 MS.gene031746:CDS
GTCAATGCCGACAACATCGG+TGG 0.615987 4.3:+31047977 None:intergenic
TTTCGGAGGATCTTCCCAGT+CGG 0.617027 4.3:+31045776 None:intergenic
AGAGGGCCTTCTATTATGAG+AGG 0.618979 4.3:-31047093 MS.gene031746:intron
AGATACCAAGGTCTCCGACT+GGG 0.620788 4.3:-31045790 MS.gene031746:CDS
GCAGGTTGCTCCATACAAGA+AGG 0.622353 4.3:-31045837 MS.gene031746:intron
TCGACTGCAGTGGGAACACG+TGG 0.624008 4.3:-31047243 MS.gene031746:CDS
AGCTCACCGCAGCTGCCTGG+GGG 0.641341 4.3:+31047123 None:intergenic
AGGGCTAAAACCCCATCATG+AGG 0.644178 4.3:+31048724 None:intergenic
AGTGCCTGAGTTGCTTTCGG+AGG 0.646420 4.3:+31045762 None:intergenic
ATGACTGAGTCGACTGCAGT+GGG 0.649133 4.3:-31047252 MS.gene031746:CDS
TCCATACAAGAAGGTCCGGA+AGG 0.659172 4.3:-31045828 MS.gene031746:CDS
TGTACTTTCTCCGAAGCATG+TGG 0.668913 4.3:-31045995 MS.gene031746:CDS
AATGACTGAGTCGACTGCAG+TGG 0.680151 4.3:-31047253 MS.gene031746:CDS
AAGAAACTAGAGCTATTAGG+TGG 0.680245 4.3:-31048427 MS.gene031746:CDS
GAGGAAATTTGACATTGACG+AGG 0.683873 4.3:-31048047 MS.gene031746:CDS
ATGAATACCAATGAGGGTGA+GGG 0.691317 4.3:+31048836 None:intergenic
AGAAATTGGGGAAATTCCAG+TGG 0.696561 4.3:-31046043 MS.gene031746:CDS
TCATCACCGACAAGATACCA+AGG 0.747173 4.3:-31045802 MS.gene031746:CDS
GTTGTTGGCTCCAAACACGG+AGG 0.795141 4.3:-31047181 MS.gene031746:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATAATTAAATGAAATAAT+CGG + chr4.3:31046936-31046955 None:intergenic 10.0%
!!! TATTTTTTTTTTTTTTCTTC+TGG - chr4.3:31048778-31048797 MS.gene031746:CDS 10.0%
!!! GTTTGATTAACAATTTTAAT+TGG + chr4.3:31048085-31048104 None:intergenic 15.0%
!!! TTATTAACATTTTACTGATA+AGG + chr4.3:31047880-31047899 None:intergenic 15.0%
!!! TTATTTTTCTTTGTTATTGT+TGG - chr4.3:31046897-31046916 MS.gene031746:intron 15.0%
!! AAAGACATTATCAAATACAA+GGG - chr4.3:31048240-31048259 MS.gene031746:CDS 20.0%
!! AATCAAATTTACCATAAAGA+AGG + chr4.3:31046308-31046327 None:intergenic 20.0%
!! AGATATTAAAAAGTGAAACA+CGG - chr4.3:31047591-31047610 MS.gene031746:intron 20.0%
!! ATATAGAGCAAATATAGTAA+AGG + chr4.3:31047708-31047727 None:intergenic 20.0%
!! ATATCAACAATTGATAAAGA+TGG - chr4.3:31048150-31048169 MS.gene031746:CDS 20.0%
!! TATAGAGCAAATATAGTAAA+GGG + chr4.3:31047707-31047726 None:intergenic 20.0%
!! TCATAGAAATCTAATTTCTA+TGG - chr4.3:31046419-31046438 MS.gene031746:CDS 20.0%
!! TTAATAGCAACTGTTATATA+GGG - chr4.3:31046979-31046998 MS.gene031746:intron 20.0%
!! TTTAATAGCAACTGTTATAT+AGG - chr4.3:31046978-31046997 MS.gene031746:intron 20.0%
!!! AGATATTGTTTATTTTGATC+AGG - chr4.3:31048188-31048207 MS.gene031746:CDS 20.0%
!!! AGTTTTTTTAACCTTCTTTA+TGG - chr4.3:31046294-31046313 MS.gene031746:intron 20.0%
!!! CAAATAAAGTTCAACTTTTA+TGG + chr4.3:31047296-31047315 None:intergenic 20.0%
! AAACAACTTTGAGTAAGAAA+TGG + chr4.3:31045962-31045981 None:intergenic 25.0%
! AAACTGAAGTAAATTTCTCT+TGG - chr4.3:31047967-31047986 MS.gene031746:CDS 25.0%
! AAATAAGCTGAAAAGAACTA+AGG - chr4.3:31047155-31047174 MS.gene031746:CDS 25.0%
! AACTAACATGATATTTCTCA+AGG + chr4.3:31047759-31047778 None:intergenic 25.0%
! AATAAATTGTTTAGCCAAAC+AGG + chr4.3:31047005-31047024 None:intergenic 25.0%
! ACAGGATAGTAAATTGAATT+TGG + chr4.3:31046060-31046079 None:intergenic 25.0%
! ATAGAGCAAATATAGTAAAG+GGG + chr4.3:31047706-31047725 None:intergenic 25.0%
! ATATAAAGGTCTTGATTCAA+TGG - chr4.3:31047619-31047638 MS.gene031746:intron 25.0%
! ATTAAGGCTATTCTATTCAA+AGG + chr4.3:31046824-31046843 None:intergenic 25.0%
! GAAAGACATTATCAAATACA+AGG - chr4.3:31048239-31048258 MS.gene031746:CDS 25.0%
! TTACTTGCATTCTCTAATTA+AGG + chr4.3:31046840-31046859 None:intergenic 25.0%
! TTATAATCAAATGTCCAAAG+AGG + chr4.3:31048531-31048550 None:intergenic 25.0%
!! ATTTGTTAACGCTTAACTAA+AGG - chr4.3:31047726-31047745 MS.gene031746:intron 25.0%
!! TGTAAGATTGTTTCCTTTTA+GGG - chr4.3:31047355-31047374 MS.gene031746:intron 25.0%
!! TTTTTGATGAATACCAATGA+GGG + chr4.3:31045810-31045829 None:intergenic 25.0%
!!! ATTGTTTATTTTGATCAGGA+TGG - chr4.3:31048192-31048211 MS.gene031746:CDS 25.0%
!!! ATTTGATAATGTCTTTCAAG+CGG + chr4.3:31048236-31048255 None:intergenic 25.0%
!!! TAAATTTGATTTGCCTCAAA+GGG - chr4.3:31046317-31046336 MS.gene031746:intron 25.0%
!!! TTTTTTTTTTTTCTTCTGGC+AGG - chr4.3:31048782-31048801 MS.gene031746:CDS 25.0%
AAACCATACACATGAAACAT+TGG + chr4.3:31046519-31046538 None:intergenic 30.0%
AACTCTTTCCTTTAATTCTC+AGG - chr4.3:31048102-31048121 MS.gene031746:CDS 30.0%
AAGGAAACAATGTCAAGAAA+AGG + chr4.3:31045880-31045899 None:intergenic 30.0%
ACCTGTATAAAATCAACGTT+AGG + chr4.3:31046795-31046814 None:intergenic 30.0%
AGGAAACAATGTCAAGAAAA+GGG + chr4.3:31045879-31045898 None:intergenic 30.0%
ATGAGGATGTGAAAAAATGA+AGG + chr4.3:31045899-31045918 None:intergenic 30.0%
CAACAATTGATAAAGATGGT+TGG - chr4.3:31048154-31048173 MS.gene031746:CDS 30.0%
CATTGGTATTCATCAAAAAC+TGG - chr4.3:31045811-31045830 MS.gene031746:CDS 30.0%
CCTGTATAAAATCAACGTTA+GGG + chr4.3:31046794-31046813 None:intergenic 30.0%
CTGGTATATTGTATTCTTCT+GGG - chr4.3:31046364-31046383 MS.gene031746:intron 30.0%
GAAAAATGACGTAACATTTG+AGG + chr4.3:31048554-31048573 None:intergenic 30.0%
GAAACACGGTAGTAATATAA+AGG - chr4.3:31047605-31047624 MS.gene031746:intron 30.0%
TATCAAATACAAGGGATTTC+AGG - chr4.3:31048248-31048267 MS.gene031746:CDS 30.0%
TCAAAGGGTAGTTATTAAAC+AGG - chr4.3:31046332-31046351 MS.gene031746:intron 30.0%
TCAAGCAATCATATAAACGA+TGG + chr4.3:31046867-31046886 None:intergenic 30.0%
TCAAGTATCCTGAGAATTAA+AGG + chr4.3:31048113-31048132 None:intergenic 30.0%
TCAGGATGGATATTTACATT+TGG - chr4.3:31048206-31048225 MS.gene031746:CDS 30.0%
TGTTTAATAACTACCCTTTG+AGG + chr4.3:31046333-31046352 None:intergenic 30.0%
TTACTATCCTGTTATACTCT+TGG - chr4.3:31046068-31046087 MS.gene031746:intron 30.0%
TTCACAACTATTCGTCAATA+GGG - chr4.3:31047434-31047453 MS.gene031746:intron 30.0%
TTCATATACTTACGCTGTAA+CGG + chr4.3:31048442-31048461 None:intergenic 30.0%
TTCTTACCTCTCATAATAGA+AGG + chr4.3:31047553-31047572 None:intergenic 30.0%
TTTCACAACTATTCGTCAAT+AGG - chr4.3:31047433-31047452 MS.gene031746:intron 30.0%
! CTGTAAGATTGTTTCCTTTT+AGG - chr4.3:31047354-31047373 MS.gene031746:intron 30.0%
! GTTTTTGATGAATACCAATG+AGG + chr4.3:31045811-31045830 None:intergenic 30.0%
!! GATTTCAGGTATCTACTTTT+AGG - chr4.3:31048262-31048281 MS.gene031746:CDS 30.0%
!! GTAAATTTGATTTGCCTCAA+AGG - chr4.3:31046316-31046335 MS.gene031746:intron 30.0%
!! TCTAGTTTCTTGACATTTTC+AGG + chr4.3:31046201-31046220 None:intergenic 30.0%
!!! ATTGTTTCCTTTTAGGGTTA+TGG - chr4.3:31047361-31047380 MS.gene031746:intron 30.0%
!!! ATTTTTTCACATCCTCATGA+TGG - chr4.3:31045901-31045920 MS.gene031746:intron 30.0%
!!! TTGAGTGATCATGTTGTTTT+GGG + chr4.3:31045779-31045798 None:intergenic 30.0%
!!! TTTTTTCACATCCTCATGAT+GGG - chr4.3:31045902-31045921 MS.gene031746:intron 30.0%
AACAGCTAGAAACACATTCT+TGG + chr4.3:31046493-31046512 None:intergenic 35.0%
AAGAAACTAGAGCTATTAGG+TGG - chr4.3:31046210-31046229 MS.gene031746:intron 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr4.3:31046123-31046142 None:intergenic 35.0%
ACCCAGATGAAGAATCAATA+AGG + chr4.3:31045936-31045955 None:intergenic 35.0%
AGCAAATATAGTAAAGGGGA+GGG + chr4.3:31047702-31047721 None:intergenic 35.0%
AGTCATTCCATAACCCTAAA+AGG + chr4.3:31047371-31047390 None:intergenic 35.0%
ATAGCGAACAACAACAATCA+AGG - chr4.3:31047924-31047943 MS.gene031746:CDS 35.0%
ATGATATTTCTCAAGGACAC+AGG + chr4.3:31047752-31047771 None:intergenic 35.0%
ATTAAACAGGAAGATACTGC+TGG - chr4.3:31046345-31046364 MS.gene031746:intron 35.0%
ATTTCACCAACACTACTAAG+AGG + chr4.3:31046132-31046151 None:intergenic 35.0%
CAGAAAATGAGAAGGATTTG+AGG - chr4.3:31046256-31046275 MS.gene031746:CDS 35.0%
CAGCAAGACAAGAAAAAAGA+AGG + chr4.3:31048054-31048073 None:intergenic 35.0%
CCCAGATGAAGAATCAATAA+GGG + chr4.3:31045935-31045954 None:intergenic 35.0%
CTAATAACTCCTATGCTCAA+AGG + chr4.3:31046753-31046772 None:intergenic 35.0%
CTCAGGATACTTGAATAATG+AGG - chr4.3:31048119-31048138 MS.gene031746:CDS 35.0%
GATCTACTGTCATTGTAATG+AGG - chr4.3:31046571-31046590 MS.gene031746:intron 35.0%
GCTGGTATATTGTATTCTTC+TGG - chr4.3:31046363-31046382 MS.gene031746:intron 35.0%
GCTTCTGCTTTACACAAAAT+GGG - chr4.3:31046000-31046019 MS.gene031746:CDS 35.0%
GTCAAGAAACTAGAGCTATT+AGG - chr4.3:31046207-31046226 MS.gene031746:intron 35.0%
GTTTCCTTTAGTCAAATCCA+TGG - chr4.3:31045978-31045997 MS.gene031746:CDS 35.0%
TCACATCCACAACAAATTCT+AGG - chr4.3:31047270-31047289 MS.gene031746:intron 35.0%
TTCCCCAATTTCTTCATCTT+CGG + chr4.3:31048587-31048606 None:intergenic 35.0%
TTGAATCCTCTTAGTAGTGT+TGG - chr4.3:31046123-31046142 MS.gene031746:intron 35.0%
TTGAGCATAGGAGTTATTAG+TGG - chr4.3:31046753-31046772 MS.gene031746:intron 35.0%
TTGCCAATGTTTCATGTGTA+TGG - chr4.3:31046513-31046532 MS.gene031746:CDS 35.0%
! AGAAAAAAGAAGGTACAGGT+AGG + chr4.3:31048044-31048063 None:intergenic 35.0%
! CTCAAATCCTTCTCATTTTC+TGG + chr4.3:31046258-31046277 None:intergenic 35.0%
! GACAAGAAAAAAGAAGGTAC+AGG + chr4.3:31048048-31048067 None:intergenic 35.0%
! GCTGCATTTTCTATAGATTG+CGG + chr4.3:31048487-31048506 None:intergenic 35.0%
! TGTTGGTGAAATTCGTAAAC+AGG - chr4.3:31046140-31046159 MS.gene031746:intron 35.0%
! TTTTTCACATCCTCATGATG+GGG - chr4.3:31045903-31045922 MS.gene031746:intron 35.0%
!! AATCTTCTAAAGTGCCTCTT+TGG - chr4.3:31048514-31048533 MS.gene031746:CDS 35.0%
!! AGAGGCACTTTAGAAGATTA+CGG + chr4.3:31048513-31048532 None:intergenic 35.0%
!! ATTTTCTGGCACAGCAATAA+TGG + chr4.3:31046244-31046263 None:intergenic 35.0%
!! CCCTTATTGATTCTTCATCT+GGG - chr4.3:31045932-31045951 MS.gene031746:intron 35.0%
!! GTCGTTTTCATAAGCTATCT+TGG - chr4.3:31047121-31047140 MS.gene031746:CDS 35.0%
!! GTTGAGTGATCATGTTGTTT+TGG + chr4.3:31045780-31045799 None:intergenic 35.0%
!! TTATGGCCTAGAATTTGTTG+TGG + chr4.3:31047279-31047298 None:intergenic 35.0%
!! TTTTCTGGCACAGCAATAAT+GGG + chr4.3:31046243-31046262 None:intergenic 35.0%
!!! AATCAAGGCCTTTTTTCACT+CGG - chr4.3:31047939-31047958 MS.gene031746:CDS 35.0%
!!! CCCTAACGTTGATTTTATAC+AGG - chr4.3:31046791-31046810 MS.gene031746:intron 35.0%
AAACCGTCTCGCAAAACTTA+TGG - chr4.3:31047211-31047230 MS.gene031746:CDS 40.0%
AAAGTGGTAGACTGGAACAA+TGG - chr4.3:31047481-31047500 MS.gene031746:intron 40.0%
AATCAACGTTAGGGAAAGCA+TGG + chr4.3:31046785-31046804 None:intergenic 40.0%
ACATAATCGACCACATGCTT+CGG + chr4.3:31048655-31048674 None:intergenic 40.0%
ACTGTTATATAGGGCCTGTT+TGG - chr4.3:31046988-31047007 MS.gene031746:intron 40.0%
AGAAATTGGGGAAATTCCAG+TGG - chr4.3:31048594-31048613 MS.gene031746:CDS 40.0%
ATGAATACCAATGAGGGTGA+GGG + chr4.3:31045804-31045823 None:intergenic 40.0%
ATGTCAAGAAAAGGGTCAGT+TGG + chr4.3:31045871-31045890 None:intergenic 40.0%
ATTGTCAATGCCGACAACAT+CGG + chr4.3:31046666-31046685 None:intergenic 40.0%
CACAAAATGGGTGTTTCACA+AGG - chr4.3:31046012-31046031 MS.gene031746:CDS 40.0%
CTATTCGTCAATAGGGTTGT+TGG - chr4.3:31047441-31047460 MS.gene031746:intron 40.0%
CTCTTGGCTGTTCTGTATTT+AGG - chr4.3:31046084-31046103 MS.gene031746:intron 40.0%
GAAGCCATGGATTTGACTAA+AGG + chr4.3:31045985-31046004 None:intergenic 40.0%
GAGCAAATATAGTAAAGGGG+AGG + chr4.3:31047703-31047722 None:intergenic 40.0%
GAGGAAATTTGACATTGACG+AGG - chr4.3:31046590-31046609 MS.gene031746:intron 40.0%
GGCTTCTGCTTTACACAAAA+TGG - chr4.3:31045999-31046018 MS.gene031746:CDS 40.0%
GTGCCGAAGATGAAGAAATT+GGG - chr4.3:31048581-31048600 MS.gene031746:CDS 40.0%
GTTGTGGATGTGAACATCAA+TGG + chr4.3:31047263-31047282 None:intergenic 40.0%
TACAGGTAGGCTAAGGAATA+AGG + chr4.3:31048031-31048050 None:intergenic 40.0%
TGACATTGACGAGGTTATCA+GGG - chr4.3:31046599-31046618 MS.gene031746:intron 40.0%
TGCCGAAGATGAAGAAATTG+GGG - chr4.3:31048582-31048601 MS.gene031746:CDS 40.0%
TTCTTGACAACAAATGCCAC+TGG + chr4.3:31048613-31048632 None:intergenic 40.0%
TTGACATTGACGAGGTTATC+AGG - chr4.3:31046598-31046617 MS.gene031746:intron 40.0%
TTTGTGTAAAGCAGAAGCCA+TGG + chr4.3:31045998-31046017 None:intergenic 40.0%
! AAAGATGGTTGGCTACATAC+AGG - chr4.3:31048165-31048184 MS.gene031746:CDS 40.0%
! AGAACAGCCAAGAGTATAAC+AGG + chr4.3:31046078-31046097 None:intergenic 40.0%
! AGTGCCGAAGATGAAGAAAT+TGG - chr4.3:31048580-31048599 MS.gene031746:CDS 40.0%
! ATAAGTTTTGCGAGACGGTT+TGG + chr4.3:31047212-31047231 None:intergenic 40.0%
! GAGCAGGTTCTTCTTTTGAT+TGG - chr4.3:31048679-31048698 MS.gene031746:CDS 40.0%
! GCACTTTAGAAGATTACGGA+TGG + chr4.3:31048509-31048528 None:intergenic 40.0%
! GCATTGACAATGAAGGCAAA+GGG - chr4.3:31046675-31046694 MS.gene031746:intron 40.0%
! TAAGTTGCAGAGTTTGAGAC+AGG - chr4.3:31046710-31046729 MS.gene031746:intron 40.0%
! TAAGTTTTGCGAGACGGTTT+GGG + chr4.3:31047211-31047230 None:intergenic 40.0%
! TTTTCCCACCGAGTGAAAAA+AGG + chr4.3:31047950-31047969 None:intergenic 40.0%
!! AGTGGCATTTGTTGTCAAGA+AGG - chr4.3:31048612-31048631 MS.gene031746:CDS 40.0%
!! ATGAAGGCAAAGGGTGTTAA+TGG - chr4.3:31046684-31046703 MS.gene031746:intron 40.0%
!! GCATTTGTTGTCAAGAAGGT+TGG - chr4.3:31048616-31048635 MS.gene031746:CDS 40.0%
!! GCCCTTATTGATTCTTCATC+TGG - chr4.3:31045931-31045950 MS.gene031746:intron 40.0%
!! TTGTATTCTTCTGGGACTAC+AGG - chr4.3:31046372-31046391 MS.gene031746:intron 40.0%
!!! AATGGTACTTTTTTACCCCC+AGG - chr4.3:31047499-31047518 MS.gene031746:intron 40.0%
AACACAGCTTCTAGATCAGC+AGG + chr4.3:31048390-31048409 None:intergenic 45.0%
AGAGGGCCTTCTATTATGAG+AGG - chr4.3:31047544-31047563 MS.gene031746:intron 45.0%
CAAGAAAAGGGTCAGTTGGA+AGG + chr4.3:31045867-31045886 None:intergenic 45.0%
CGTAAACAGGTTCAAGAGTG+TGG - chr4.3:31046153-31046172 MS.gene031746:intron 45.0%
GATGAATACCAATGAGGGTG+AGG + chr4.3:31045805-31045824 None:intergenic 45.0%
GGGAGGGAGTCTTGATATAA+AGG + chr4.3:31047686-31047705 None:intergenic 45.0%
TCATCACCGACAAGATACCA+AGG - chr4.3:31048835-31048854 MS.gene031746:CDS 45.0%
TGCTGTGCCAGAAAATGAGA+AGG - chr4.3:31046248-31046267 MS.gene031746:CDS 45.0%
TGGTTTATCGCTCTTTGCTG+CGG - chr4.3:31046533-31046552 MS.gene031746:intron 45.0%
TGTACTTTCTCCGAAGCATG+TGG - chr4.3:31048642-31048661 MS.gene031746:CDS 45.0%
TGTTGTTCCACCGATGTTGT+CGG - chr4.3:31046653-31046672 MS.gene031746:intron 45.0%
TTGCTCCATACAAGAAGGTC+CGG - chr4.3:31048805-31048824 MS.gene031746:CDS 45.0%
! AAGAAGGTACAGGTAGGCTA+AGG + chr4.3:31048038-31048057 None:intergenic 45.0%
! AGTTTGAGACAGGTCTCTTG+CGG - chr4.3:31046720-31046739 MS.gene031746:intron 45.0%
! CTGCCATAAGTTTTGCGAGA+CGG + chr4.3:31047217-31047236 None:intergenic 45.0%
!! AAAGGTTTGAGTTGCTGCAG+CGG + chr4.3:31047668-31047687 None:intergenic 45.0%
!! CTTCATCTTCGGCACTGATA+TGG + chr4.3:31048576-31048595 None:intergenic 45.0%
!! GGCATTGACAATGAAGGCAA+AGG - chr4.3:31046674-31046693 MS.gene031746:intron 45.0%
!! GTTGTCGGCATTGACAATGA+AGG - chr4.3:31046668-31046687 MS.gene031746:intron 45.0%
!!! AAGGCCTTTTTTCACTCGGT+GGG - chr4.3:31047943-31047962 MS.gene031746:CDS 45.0%
!! AAGAAAAAAAAAAAAAATAA+AGG + chr4.3:31048777-31048796 None:intergenic 5.0%
!! AGAAAAAAAAAAAAAATAAA+GGG + chr4.3:31048776-31048795 None:intergenic 5.0%
AAGATACCAAGGTCTCCGAC+TGG - chr4.3:31048846-31048865 MS.gene031746:CDS 50.0%
AATGACTGAGTCGACTGCAG+TGG - chr4.3:31047384-31047403 MS.gene031746:intron 50.0%
ACCTTCCGGACCTTCTTGTA+TGG + chr4.3:31048813-31048832 None:intergenic 50.0%
AGATACCAAGGTCTCCGACT+GGG - chr4.3:31048847-31048866 MS.gene031746:CDS 50.0%
AGATCCTCCGAAAGCAACTC+AGG - chr4.3:31048871-31048890 MS.gene031746:CDS 50.0%
AGGGCTAAAACCCCATCATG+AGG + chr4.3:31045916-31045935 None:intergenic 50.0%
ATGACTGAGTCGACTGCAGT+GGG - chr4.3:31047385-31047404 MS.gene031746:intron 50.0%
CGGAGACCTTGGTATCTTGT+CGG + chr4.3:31048844-31048863 None:intergenic 50.0%
GACAACATCGGTGGAACAAC+AGG + chr4.3:31046654-31046673 None:intergenic 50.0%
GCAGGTTGCTCCATACAAGA+AGG - chr4.3:31048800-31048819 MS.gene031746:CDS 50.0%
GCTGCGGGATTATTGTCGTT+GGG - chr4.3:31046549-31046568 MS.gene031746:intron 50.0%
GGGACTACAGGTGTGAGTAA+AGG - chr4.3:31046384-31046403 MS.gene031746:intron 50.0%
GGGTCAGTTGGAAGGTTTCT+AGG + chr4.3:31045859-31045878 None:intergenic 50.0%
GGTCGATTATGTTGCTGAGC+AGG - chr4.3:31048663-31048682 MS.gene031746:CDS 50.0%
GGTTTATCGCTCTTTGCTGC+GGG - chr4.3:31046534-31046553 MS.gene031746:intron 50.0%
TCCAAACACGGAGGCAAAAG+TGG - chr4.3:31047465-31047484 MS.gene031746:intron 50.0%
TCCATACAAGAAGGTCCGGA+AGG - chr4.3:31048809-31048828 MS.gene031746:CDS 50.0%
TGCTGCGGGATTATTGTCGT+TGG - chr4.3:31046548-31046567 MS.gene031746:intron 50.0%
TGTCGGTGATGAACACCTTC+CGG + chr4.3:31048827-31048846 None:intergenic 50.0%
TTTCGGAGGATCTTCCCAGT+CGG + chr4.3:31048864-31048883 None:intergenic 50.0%
! AGCAGTGCCTGAGTTGCTTT+CGG + chr4.3:31048881-31048900 None:intergenic 50.0%
!! ACCACTTTTGCCTCCGTGTT+TGG + chr4.3:31047469-31047488 None:intergenic 50.0%
!! ACGGCTGCATCGACTATTTC+AGG + chr4.3:31048423-31048442 None:intergenic 50.0%
!! AGGGTTGTTGGCTCCAAACA+CGG - chr4.3:31047453-31047472 MS.gene031746:intron 50.0%
!!! CAAGGCCTTTTTTCACTCGG+TGG - chr4.3:31047942-31047961 MS.gene031746:CDS 50.0%
ATCTTCCCAGTCGGAGACCT+TGG + chr4.3:31048855-31048874 None:intergenic 55.0%
CAGCGGTGTGACTAACGTTC+AGG + chr4.3:31047651-31047670 None:intergenic 55.0%
CGGAGGCAAAAGTGGTAGAC+TGG - chr4.3:31047473-31047492 MS.gene031746:intron 55.0%
CTCAACACCCTCACCCTCAT+TGG - chr4.3:31045794-31045813 MS.gene031746:CDS 55.0%
GTCAATGCCGACAACATCGG+TGG + chr4.3:31046663-31046682 None:intergenic 55.0%
! AGTGCCTGAGTTGCTTTCGG+AGG + chr4.3:31048878-31048897 None:intergenic 55.0%
! GCGGTGAGCTTTGGTTGAGA+GGG - chr4.3:31047527-31047546 MS.gene031746:intron 55.0%
! TGCGGTGAGCTTTGGTTGAG+AGG - chr4.3:31047526-31047545 MS.gene031746:intron 55.0%
! TTTTTACCCCCAGGCAGCTG+CGG - chr4.3:31047508-31047527 MS.gene031746:intron 55.0%
!! GTTGTTGGCTCCAAACACGG+AGG - chr4.3:31047456-31047475 MS.gene031746:intron 55.0%
AAAGCTCACCGCAGCTGCCT+GGG + chr4.3:31047519-31047538 None:intergenic 60.0%
GGTGCAGCGCCTTTGAGCAT+AGG - chr4.3:31046741-31046760 MS.gene031746:intron 60.0%
TCGACTGCAGTGGGAACACG+TGG - chr4.3:31047394-31047413 MS.gene031746:intron 60.0%
AAGCTCACCGCAGCTGCCTG+GGG + chr4.3:31047518-31047537 None:intergenic 65.0%
CAAAGCTCACCGCAGCTGCC+TGG + chr4.3:31047520-31047539 None:intergenic 65.0%
CAGGCAGCTGCGGTGAGCTT+TGG - chr4.3:31047518-31047537 MS.gene031746:intron 65.0%
AGCTCACCGCAGCTGCCTGG+GGG + chr4.3:31047517-31047536 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 31045742 31048917 31045742 ID=MS.gene031746
chr4.3 mRNA 31045742 31048917 31045742 ID=MS.gene031746.t1;Parent=MS.gene031746
chr4.3 exon 31047847 31048917 31047847 ID=MS.gene031746.t1.exon1;Parent=MS.gene031746.t1
chr4.3 CDS 31047847 31048917 31047847 ID=cds.MS.gene031746.t1;Parent=MS.gene031746.t1
chr4.3 exon 31047094 31047283 31047094 ID=MS.gene031746.t1.exon2;Parent=MS.gene031746.t1
chr4.3 CDS 31047094 31047283 31047094 ID=cds.MS.gene031746.t1;Parent=MS.gene031746.t1
chr4.3 exon 31046390 31046535 31046390 ID=MS.gene031746.t1.exon3;Parent=MS.gene031746.t1
chr4.3 CDS 31046390 31046535 31046390 ID=cds.MS.gene031746.t1;Parent=MS.gene031746.t1
chr4.3 exon 31046211 31046278 31046211 ID=MS.gene031746.t1.exon4;Parent=MS.gene031746.t1
chr4.3 CDS 31046211 31046278 31046211 ID=cds.MS.gene031746.t1;Parent=MS.gene031746.t1
chr4.3 exon 31045975 31046077 31045975 ID=MS.gene031746.t1.exon5;Parent=MS.gene031746.t1
chr4.3 CDS 31045975 31046077 31045975 ID=cds.MS.gene031746.t1;Parent=MS.gene031746.t1
chr4.3 exon 31045742 31045855 31045742 ID=MS.gene031746.t1.exon6;Parent=MS.gene031746.t1
chr4.3 CDS 31045742 31045855 31045742 ID=cds.MS.gene031746.t1;Parent=MS.gene031746.t1
Gene Sequence

>MS.gene031746

ATGGCATCTGCTACAAATTTCAACTTCGAAATCACCCAAAACAACATGATCACTCAACACCCTCACCCTCATTGGTATTCATCAAAAACTGGAATTTACAACAGTATACACTCTCCTAGAAACCTTCCAACTGACCCTTTTCTTGACATTGTTTCCTTCATTTTTTCACATCCTCATGATGGGGTTTTAGCCCTTATTGATTCTTCATCTGGGTCTTCCATTTCTTACTCAAAGTTGTTTCCTTTAGTCAAATCCATGGCTTCTGCTTTACACAAAATGGGTGTTTCACAAGGTGATGTTGTTTTGCTTCTACTTCCAAATTCAATTTACTATCCTGTTATACTCTTGGCTGTTCTGTATTTAGGTGCTGTTTTTACACCATTGAATCCTCTTAGTAGTGTTGGTGAAATTCGTAAACAGGTTCAAGAGTGTGGTGTGAGTTTTGCTTTTACTATACCTGAAAATGTCAAGAAACTAGAGCTATTAGGTGGCATTAATCCCATTATTGCTGTGCCAGAAAATGAGAAGGATTTGAGGAATGAGTGTTTCTCAAGTTTTTTTAACCTTCTTTATGGTAAATTTGATTTGCCTCAAAGGGTAGTTATTAAACAGGAAGATACTGCTGGTATATTGTATTCTTCTGGGACTACAGGTGTGAGTAAAGGAGTTGTTTTAACTCATAGAAATCTAATTTCTATGGTTGAGCTTTTTGTGAGATTTGAAGCTTCTCAATATGATTATTCTTGTTCCAAGAATGTGTTTCTAGCTGTTTTGCCAATGTTTCATGTGTATGGTTTATCGCTCTTTGCTGCGGGATTATTGTCGTTGGGATCTACTGTCATTGTAATGAGGAAATTTGACATTGACGAGGTTATCAGGGTGATTGACAAATATAATGTTACACACTTTCCTGTTGTTCCACCGATGTTGTCGGCATTGACAATGAAGGCAAAGGGTGTTAATGGAAGTAAGTTGCAGAGTTTGAGACAGGTCTCTTGCGGTGCAGCGCCTTTGAGCATAGGAGTTATTAGTGGTTTTGTCCATGCTTTCCCTAACGTTGATTTTATACAGGTAAATAACCTTTGAATAGAATAGCCTTAATTAGAGAATGCAAGTAATGCTCCATCGTTTATATGATTGCTTGAGTTAATATAGTTATTTTTCTTTGTTATTGTTGGTAGTAGCTAAATGCCGATTATTTCATTTAATTATGAGAGAACGTGTGAGCTTTAAAAATTTAATAGCAACTGTTATATAGGGCCTGTTTGGCTAAACAATTTATTTGCTGCTTATCGCATAAGCACTTTTTATACACTTACATAAGCTATTTCTATAGCAAAAAATAAAATAAAGTAAAATTGTTTTTGTATATGTTAAAAGTCGTTTTCATAAGCTATCTTGGACAACTTATAAAAATAAGCTGAAAAGAACTAAGGAAAAATGTCAAAGTTGTTCTCATAAGTTCTCCCAAACCGTCTCGCAAAACTTATGGCAGCAGATAAGCTCAAATAAGTTGTGCCATTGATGTTCACATCCACAACAAATTCTAGGCCATAAAAGTTGAACTTTATTTGTTTATGATAATAATGTTGTTTTACTAGATTATTAATAGCTGTAAGATTGTTTCCTTTTAGGGTTATGGAATGACTGAGTCGACTGCAGTGGGAACACGTGGCTTCAATACTGAAAAGTTTCACAACTATTCGTCAATAGGGTTGTTGGCTCCAAACACGGAGGCAAAAGTGGTAGACTGGAACAATGGTACTTTTTTACCCCCAGGCAGCTGCGGTGAGCTTTGGTTGAGAGGGCCTTCTATTATGAGAGGTAAGAAATCTGCATTTTTCTTAATAGATATTAAAAAGTGAAACACGGTAGTAATATAAAGGTCTTGATTCAATGGCTAGAACCTGAACGTTAGTCACACCGCTGCAGCAACTCAAACCTTTATATCAAGACTCCCTCCCCTTTACTATATTTGCTCTATATTTGTTAACGCTTAACTAAAGGCCTGTGTCCTTGAGAAATATCATGTTAGTTTAGTAGACATTTTATTCTTCTAGAGTGATGCTATACACTCATCTGTTGCTGTAGTGCAACTATTGACGCGAATTCAGTTGAAAGATACATATACATCTCCTTATCAGTAAAATGTTAATAAAAGTTTTCTTTCTATTTAGTTTAGATAGCGAACAACAACAATCAAGGCCTTTTTTCACTCGGTGGGAAAACTGAAGTAAATTTCTCTTGGAGTCAACTGTTTCATTGCTATAATAACTTTTGTTTCTGCCTTATTCCTTAGCCTACCTGTACCTTCTTTTTTCTTGTCTTGCTGCTATTCGTCCAATTAAAATTGTTAATCAAACTCTTTCCTTTAATTCTCAGGATACTTGAATAATGAGGAAGCTACTATATCAACAATTGATAAAGATGGTTGGCTACATACAGGAGATATTGTTTATTTTGATCAGGATGGATATTTACATTTGGCTGACCGCTTGAAAGACATTATCAAATACAAGGGATTTCAGGTATCTACTTTTAGGTTTATTAAATTTTATAAAGAAAAGCGATGATAGATTAACGAGAAATGCAGAATTTTACTGAAACGCACACTTTGTATGACACATGATCTGTTACAGATTGCTCCTGCTGATCTAGAAGCTGTGTTAATTTTGCATCCTGAAATAGTCGATGCAGCCGTTACAGCGTAAGTATATGAACACTTCATTTATGTCTTCGCTTCCGCAATCTATAGAAAATGCAGCCATCCGTAATCTTCTAAAGTGCCTCTTTGGACATTTGATTATAACCTCAAATGTTACGTCATTTTTCCATATCAGTGCCGAAGATGAAGAAATTGGGGAAATTCCAGTGGCATTTGTTGTCAAGAAGGTTGGAAGTGTACTTTCTCCGAAGCATGTGGTCGATTATGTTGCTGAGCAGGTTCTTCTTTTGATTGGACAGACATGATTTTTATGATATTTGAATTGATATTACTACTTGAATCAATGTCATGCTTTTCACATTTTAACCCTTTATTTTTTTTTTTTTTCTTCTGGCAGGTTGCTCCATACAAGAAGGTCCGGAAGGTGTTCATCACCGACAAGATACCAAGGTCTCCGACTGGGAAGATCCTCCGAAAGCAACTCAGGCACTGCTTGAGTTCTAAACTCTAA
Protein sequence

>MS.gene031746.t1

MASATNFNFEITQNNMITQHPHPHWYSSKTGIYNSIHSPRNLPTDPFLDIVSFIFSHPHDGVLALIDSSSGSSISYSKLFPLVKSMASALHKMGVSQGDVVLLLLPNSIYYPVILLAVLYLGAVFTPLNPLSSVGEIRKQVQECGVSFAFTIPENVKKLELLGGINPIIAVPENEKDLRNECFSSFFNLLYGKFDLPQRVVIKQEDTAGILYSSGTTGVSKGVVLTHRNLISMVELFVRFEASQYDYSCSKNVFLAVLPMFHVYGLSLFAAGLLSLGSTVIVMRKFDIDEVIRVIDKYNVTHFPVVPPMLSALTMKAKGVNGSKLQSLRQVSCGAAPLSIGVISGFVHAFPNVDFIQGYGMTESTAVGTRGFNTEKFHNYSSIGLLAPNTEAKVVDWNNGTFLPPGSCGELWLRGPSIMRGYLNNEEATISTIDKDGWLHTGDIVYFDQDGYLHLADRLKDIIKYKGFQIAPADLEAVLILHPEIVDAAVTAAEDEEIGEIPVAFVVKKVGSVLSPKHVVDYVAEQVAPYKKVRKVFITDKIPRSPTGKILRKQLRHCLSSKL