AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72872

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72872.t1 MTR_5g027500 95.775 568 24 0 1 568 1 568 0.0 1099
MS.gene72872.t1 MTR_5g027480 82.218 568 88 2 1 568 1 555 0.0 942
MS.gene72872.t1 MTR_4g077900 81.224 490 78 2 81 568 1 478 0.0 804
MS.gene72872.t1 MTR_1g026310 43.063 555 297 6 23 568 16 560 1.73e-153 452
MS.gene72872.t1 MTR_8g039720 43.494 561 304 7 12 568 8 559 2.01e-152 449
MS.gene72872.t1 MTR_1g090737 45.848 554 264 12 21 560 27 558 2.93e-145 431
MS.gene72872.t1 MTR_4g057730 41.423 548 294 9 21 560 6 534 2.02e-137 410
MS.gene72872.t1 MTR_2g095510 39.526 549 307 8 26 568 10 539 3.46e-132 396
MS.gene72872.t1 MTR_1g063120 39.965 568 307 11 19 568 4 555 3.66e-130 392
MS.gene72872.t1 MTR_3g088880 40.784 510 273 8 55 560 64 548 1.64e-129 390
MS.gene72872.t1 MTR_1g063110 39.383 551 311 7 19 560 4 540 3.79e-127 384
MS.gene72872.t1 MTR_3g088870 41.120 518 276 10 55 566 63 557 1.03e-126 383
MS.gene72872.t1 MTR_1g073180 39.850 532 275 10 43 562 30 528 7.21e-114 349
MS.gene72872.t1 MTR_4g057730 40.994 483 258 9 21 495 6 469 1.49e-112 343
MS.gene72872.t1 MTR_2g105570 40.194 515 290 8 59 566 44 547 2.61e-111 343
MS.gene72872.t1 MTR_4g128337 37.308 520 296 10 59 568 49 548 2.01e-102 320
MS.gene72872.t1 MTR_1g073180 38.402 513 271 10 43 543 52 531 7.32e-102 318
MS.gene72872.t1 MTR_4g057730 39.440 464 254 9 21 476 6 450 2.73e-101 314
MS.gene72872.t1 MTR_4g005750 39.265 517 286 10 59 566 43 540 4.67e-101 316
MS.gene72872.t1 MTR_5g007640 38.417 518 290 8 59 566 87 585 5.06e-101 318
MS.gene72872.t1 MTR_3g460770 34.111 557 343 10 17 568 4 541 8.68e-99 311
MS.gene72872.t1 MTR_2g095510 37.391 460 263 8 26 479 10 450 3.07e-96 301
MS.gene72872.t1 MTR_1g026430 36.869 396 231 6 23 409 16 401 9.44e-78 251
MS.gene72872.t1 MTR_1g026470 36.869 396 231 6 23 409 16 401 2.47e-77 250
MS.gene72872.t1 MTR_3g035130 29.058 499 320 12 69 558 32 505 3.88e-50 181
MS.gene72872.t1 MTR_5g076240 28.107 523 331 17 67 562 38 542 2.09e-44 166
MS.gene72872.t1 MTR_1569s0010 26.499 517 348 10 69 562 22 529 9.13e-44 164
MS.gene72872.t1 MTR_3g064420 26.443 537 330 15 65 561 36 547 1.55e-38 149
MS.gene72872.t1 MTR_3g035130 28.477 453 290 12 69 512 32 459 1.81e-38 147
MS.gene72872.t1 MTR_2g044180 27.308 509 328 14 81 561 58 552 2.45e-37 146
MS.gene72872.t1 MTR_8g074040 27.725 523 319 19 81 568 94 592 3.46e-37 146
MS.gene72872.t1 MTR_2g044180 29.870 385 243 10 194 561 87 461 3.62e-37 144
MS.gene72872.t1 MTR_0803s0020 28.030 528 280 16 71 554 263 734 4.37e-37 147
MS.gene72872.t1 MTR_4g011790 27.681 401 257 10 191 567 207 598 4.66e-37 145
MS.gene72872.t1 MTR_4g013210 28.792 389 234 11 210 567 69 445 6.39e-37 142
MS.gene72872.t1 MTR_5g008800 29.644 506 309 17 81 562 52 534 2.82e-36 142
MS.gene72872.t1 MTR_4g011790 27.317 410 256 12 191 567 207 607 2.87e-35 140
MS.gene72872.t1 MTR_4g011790 27.763 389 248 10 191 555 207 586 3.87e-35 140
MS.gene72872.t1 MTR_4g121600 26.577 523 336 16 70 561 47 552 7.09e-35 139
MS.gene72872.t1 MTR_8g074040 29.843 382 229 12 211 568 108 474 9.14e-34 134
MS.gene72872.t1 MTR_5g076220 28.649 370 234 10 209 561 186 542 9.17e-34 135
MS.gene72872.t1 MTR_0161s0050 28.649 370 234 10 209 561 188 544 1.11e-33 135
MS.gene72872.t1 MTR_5g008820 28.487 509 307 18 81 561 52 531 1.55e-33 134
MS.gene72872.t1 MTR_8g074120 30.151 398 215 16 196 562 124 489 1.84e-32 131
MS.gene72872.t1 MTR_2g030240 25.735 544 330 14 72 568 31 547 1.43e-31 129
MS.gene72872.t1 MTR_4g013210 27.056 377 215 9 210 567 69 404 9.69e-30 121
MS.gene72872.t1 MTR_5g076200 26.554 531 332 16 65 561 36 542 1.25e-29 123
MS.gene72872.t1 MTR_4g013210 27.056 377 215 9 210 567 221 556 3.04e-29 122
MS.gene72872.t1 MTR_5g023730 26.901 513 323 20 81 566 55 542 3.57e-29 122
MS.gene72872.t1 MTR_4g013210 28.492 358 213 11 210 536 221 566 9.88e-29 120
MS.gene72872.t1 MTR_7g084850 24.107 560 369 14 44 568 186 724 1.85e-28 120
MS.gene72872.t1 MTR_1g026350 41.791 134 78 0 21 154 20 153 1.19e-27 109
MS.gene72872.t1 MTR_2g030240 26.598 391 236 9 215 568 77 453 1.99e-26 112
MS.gene72872.t1 MTR_3g087980 24.949 493 278 16 69 488 106 579 7.27e-24 106
MS.gene72872.t1 MTR_1g030290 28.726 369 168 17 191 488 237 581 1.75e-23 105
MS.gene72872.t1 MTR_1g104810 26.776 549 313 21 7 485 39 568 3.47e-23 104
MS.gene72872.t1 MTR_1g104810 31.595 326 162 13 208 485 189 501 9.86e-23 102
MS.gene72872.t1 MTR_4g057755 55.556 72 32 0 424 495 46 117 6.84e-22 91.7
MS.gene72872.t1 MTR_1054s0020 24.655 507 342 15 66 562 294 770 5.91e-21 97.8
MS.gene72872.t1 MTR_7g109830 23.864 528 316 16 38 495 36 547 1.26e-18 90.1
MS.gene72872.t1 MTR_8g083170 31.818 220 128 7 339 545 461 671 3.51e-18 89.0
MS.gene72872.t1 MTR_2g098490 26.515 396 235 16 211 557 647 1035 4.00e-18 89.4
MS.gene72872.t1 MTR_3g082660 26.705 352 179 14 202 486 272 611 7.96e-18 87.8
MS.gene72872.t1 MTR_3g084450 35.673 171 87 7 328 481 233 397 2.26e-17 85.5
MS.gene72872.t1 MTR_3g084470 37.671 146 75 5 348 481 460 601 4.25e-17 85.5
MS.gene72872.t1 MTR_3g084450 35.673 171 87 7 328 481 441 605 6.06e-17 84.7
MS.gene72872.t1 MTR_4g013210 27.018 285 158 8 210 485 221 464 6.06e-17 84.3
MS.gene72872.t1 MTR_3g088890 48.438 64 33 0 470 533 10 73 1.34e-13 67.0
MS.gene72872.t1 MTR_4g013210 27.530 247 147 9 210 436 221 455 2.21e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72872.t1 AT4G19010 59.601 552 211 4 18 568 22 562 0.0 659
MS.gene72872.t1 AT1G20510 44.669 544 278 7 28 566 17 542 1.63e-159 467
MS.gene72872.t1 AT1G20480 42.626 556 294 8 21 568 27 565 3.23e-150 444
MS.gene72872.t1 AT1G20500 42.936 545 290 8 28 568 23 550 8.56e-144 427
MS.gene72872.t1 AT4G05160 40.835 551 306 8 24 568 8 544 8.22e-140 416
MS.gene72872.t1 AT5G38120 40.993 544 301 8 28 568 24 550 1.45e-135 405
MS.gene72872.t1 AT1G20510 43.975 473 242 7 28 495 17 471 6.59e-133 396
MS.gene72872.t1 AT5G63380 40.000 555 297 10 21 560 17 550 1.86e-131 395
MS.gene72872.t1 AT5G38120 39.961 508 285 8 28 532 24 514 1.73e-120 365
MS.gene72872.t1 AT1G20510 42.384 453 238 7 28 475 17 451 1.15e-119 362
MS.gene72872.t1 AT3G21240 40.037 547 291 12 34 568 33 554 2.92e-117 358
MS.gene72872.t1 AT5G38120 40.127 471 262 8 28 495 24 477 2.84e-110 338
MS.gene72872.t1 AT5G38120 40.127 471 262 8 28 495 24 477 2.84e-110 338
MS.gene72872.t1 AT1G51680 40.848 519 285 10 59 568 56 561 7.84e-109 337
MS.gene72872.t1 AT3G21230 39.888 534 303 8 41 566 43 566 5.68e-108 335
MS.gene72872.t1 AT1G65060 39.526 506 289 8 59 561 66 557 1.44e-102 321
MS.gene72872.t1 AT3G21230 39.600 500 284 8 41 532 43 532 3.12e-96 304
MS.gene72872.t1 AT1G51680 40.166 483 267 10 59 532 56 525 8.93e-96 302
MS.gene72872.t1 AT1G62940 36.641 524 289 9 58 566 43 538 6.59e-94 298
MS.gene72872.t1 AT1G20490 39.762 420 236 7 28 444 23 428 5.20e-93 292
MS.gene72872.t1 AT1G20490 39.762 420 236 7 28 444 23 428 5.20e-93 292
MS.gene72872.t1 AT3G21240 38.655 476 255 12 34 497 33 483 3.84e-92 291
MS.gene72872.t1 AT3G21240 38.655 476 255 12 34 497 33 483 3.84e-92 291
MS.gene72872.t1 AT3G21230 38.925 465 266 8 41 497 43 497 3.46e-86 276
MS.gene72872.t1 AT1G51680 39.062 448 251 10 59 497 56 490 2.53e-84 271
MS.gene72872.t1 AT1G65060 36.967 422 249 8 59 477 66 473 2.77e-71 237
MS.gene72872.t1 AT3G21230 34.010 394 242 8 41 426 43 426 9.34e-49 176
MS.gene72872.t1 AT3G48990 27.309 498 332 14 68 558 30 504 1.01e-43 163
MS.gene72872.t1 AT1G77240 29.755 531 311 21 68 566 43 543 6.45e-43 161
MS.gene72872.t1 AT1G65880 28.466 541 315 19 67 562 38 551 3.22e-41 157
MS.gene72872.t1 AT5G16370 28.083 527 326 17 66 562 36 539 4.07e-41 156
MS.gene72872.t1 AT1G21540 27.068 532 328 16 68 566 44 548 1.73e-40 155
MS.gene72872.t1 AT1G65890 27.156 545 316 18 67 562 38 550 1.28e-39 152
MS.gene72872.t1 AT1G21530 27.841 528 320 20 68 562 44 543 1.06e-38 149
MS.gene72872.t1 AT1G21530 27.736 530 316 22 68 562 44 541 2.02e-38 149
MS.gene72872.t1 AT1G75960 28.261 506 318 15 81 561 53 538 1.97e-37 146
MS.gene72872.t1 AT1G66120 29.207 517 306 18 81 562 52 543 8.17e-37 144
MS.gene72872.t1 AT3G16910 30.025 393 234 10 194 562 181 556 9.06e-37 144
MS.gene72872.t1 AT1G20560 28.740 508 315 17 81 562 54 540 1.49e-36 144
MS.gene72872.t1 AT5G16340 27.586 522 319 19 72 562 46 539 1.04e-35 141
MS.gene72872.t1 AT1G20560 32.021 381 226 14 198 562 99 462 5.58e-35 138
MS.gene72872.t1 AT1G30520 27.890 545 292 20 78 561 42 546 8.01e-35 139
MS.gene72872.t1 AT1G30520 27.890 545 292 20 78 561 42 546 8.01e-35 139
MS.gene72872.t1 AT2G17650 27.789 511 326 15 81 562 99 595 1.22e-33 135
MS.gene72872.t1 AT3G16170 26.546 388 241 13 210 567 234 607 6.44e-31 127
MS.gene72872.t1 AT5G36880 25.516 533 355 14 68 568 147 669 1.15e-29 124
MS.gene72872.t1 AT5G36880 25.516 533 355 14 68 568 147 669 1.15e-29 124
MS.gene72872.t1 AT5G36880 25.516 533 355 14 68 568 147 669 1.15e-29 124
MS.gene72872.t1 AT5G36880 25.516 533 355 14 68 568 147 669 1.15e-29 124
MS.gene72872.t1 AT5G36880 25.704 533 354 14 68 568 197 719 1.42e-29 124
MS.gene72872.t1 AT5G27600 27.481 524 298 17 24 481 60 567 1.56e-27 117
MS.gene72872.t1 AT1G30520 27.115 461 248 17 78 492 42 460 5.30e-27 114
MS.gene72872.t1 AT1G30520 27.115 461 248 17 78 492 42 460 5.30e-27 114
MS.gene72872.t1 AT1G68270 24.016 508 319 15 81 561 52 519 2.96e-26 113
MS.gene72872.t1 AT1G76290 27.148 512 310 22 81 561 52 531 7.05e-25 108
MS.gene72872.t1 AT5G36880 26.258 457 298 13 68 495 147 593 6.70e-24 106
MS.gene72872.t1 AT1G64400 24.765 533 315 19 22 483 21 538 6.52e-23 103
MS.gene72872.t1 AT3G05970 28.023 521 293 19 27 481 63 567 1.31e-21 99.8
MS.gene72872.t1 AT1G77590 26.961 408 202 16 191 531 87 465 2.07e-21 98.6
MS.gene72872.t1 AT4G11030 23.033 521 323 15 52 509 58 563 2.09e-21 99.0
MS.gene72872.t1 AT4G23850 26.287 369 219 11 188 509 201 563 3.18e-21 98.2
MS.gene72872.t1 AT1G77590 26.961 408 202 16 191 531 233 611 4.73e-21 97.8
MS.gene72872.t1 AT1G49430 24.806 516 301 16 36 481 39 537 1.40e-20 96.3
MS.gene72872.t1 AT3G23790 34.932 146 79 5 348 481 465 606 9.61e-15 77.8
MS.gene72872.t1 AT2G04350 22.841 521 312 16 70 518 131 633 4.84e-13 72.4
MS.gene72872.t1 AT2G04350 22.841 521 312 16 70 518 131 633 4.84e-13 72.4
MS.gene72872.t1 AT4G14070 29.268 164 99 6 348 499 481 639 8.16e-11 65.5

Find 122 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTGGTCTTCATAAAA+TGG 0.181069 5.1:-18005921 MS.gene72872:CDS
AAGGAAACAACATCGATAAA+AGG 0.225166 5.1:+18006043 None:intergenic
GCCCTTATTGATTCCTCATC+TGG 0.228419 5.1:-18005989 MS.gene72872:CDS
ATGACATCGATGAGGCTATT+AGG 0.231417 5.1:-18005292 MS.gene72872:CDS
TATCAAATACAAGGGCTTTC+AGG 0.237687 5.1:-18004225 MS.gene72872:intron
TTGCCGATGTTCCATTTATA+TGG 0.273927 5.1:-18005377 MS.gene72872:CDS
ACCTGTATAAAATCAACATT+AGG 0.277352 5.1:+18005098 None:intergenic
TGGTAAATTCTATTCAATAA+AGG 0.287765 5.1:-18005612 MS.gene72872:CDS
TTAGTCAAATCCTTGGCTTC+TGG 0.296472 5.1:-18005935 MS.gene72872:CDS
TGCTACCGGATTGTTGTCAT+TGG 0.298226 5.1:-18005342 MS.gene72872:CDS
ATAGGATAGTAAATTGAATT+AGG 0.301304 5.1:+18005863 None:intergenic
AAAGCTCACCGCTGAGGCCT+GGG 0.310423 5.1:+18004613 None:intergenic
GCTGGTATATTATATTCTTC+TGG 0.320796 5.1:-18005527 MS.gene72872:CDS
CAAAGCTCACCGCTGAGGCC+TGG 0.333831 5.1:+18004612 None:intergenic
AGAGTGATAAACCATATAAA+TGG 0.334990 5.1:+18005366 None:intergenic
AGTCAAAGTCACCATGAATA+AGG 0.340131 5.1:+18005585 None:intergenic
ACGGCGGCATCAACTATTTC+AGG 0.342047 5.1:+18004055 None:intergenic
ACTTTCCGAACCTTCTTGTA+TGG 0.345292 5.1:+18003697 None:intergenic
CAGGCCTCAGCGGTGAGCTT+TGG 0.348099 5.1:-18004611 MS.gene72872:CDS
CGAAATTCGTAAGCAAGTTA+AGG 0.354514 5.1:-18005774 MS.gene72872:CDS
GATGGATATTTATATTTGTC+TGG 0.362112 5.1:-18004263 MS.gene72872:CDS
AGCAATTCCTGAGTTGCTTT+CGG 0.373107 5.1:+18003629 None:intergenic
TAACAAATTTGAGTAAGAAA+TGG 0.376318 5.1:+18005961 None:intergenic
ATAAAGGAAAACCTTATTCA+TGG 0.384793 5.1:-18005596 MS.gene72872:CDS
TGACATCGATGAGGCTATTA+GGG 0.391194 5.1:-18005291 MS.gene72872:CDS
GTGTCTTCTTGTTTAATAAC+CGG 0.393788 5.1:+18005551 None:intergenic
GGAGATTCCAGTGGCATTTG+TGG 0.394931 5.1:-18003915 MS.gene72872:CDS
TCCCAGATGAGGAATCAATA+AGG 0.401871 5.1:+18005987 None:intergenic
ATAACACTCAATAGTACAAT+AGG 0.403610 5.1:+18005845 None:intergenic
TGCTGTGCCAGAAAATGAAA+TGG 0.427789 5.1:-18005678 MS.gene72872:CDS
GCTTCTGGTCTTCATAAAAT+GGG 0.430989 5.1:-18005920 MS.gene72872:CDS
GTCAATGCCGACAACATCAT+TGG 0.431399 5.1:+18005230 None:intergenic
CCCTTATTGATTCCTCATCT+GGG 0.431410 5.1:-18005988 MS.gene72872:CDS
TTATATTCTTCTGGCACTAC+AGG 0.438374 5.1:-18005518 MS.gene72872:CDS
TGGAGGCAAAAGTGGTAGAT+TGG 0.438591 5.1:-18004656 MS.gene72872:CDS
CCTGTATAAAATCAACATTA+GGG 0.441453 5.1:+18005099 None:intergenic
AAAGATGGTTGGCTACATAC+TGG 0.442873 5.1:-18004308 MS.gene72872:CDS
GATCGATTACGTTGCTAAGC+AGG 0.446575 5.1:-18003855 MS.gene72872:intron
GCTACCGGATTGTTGTCATT+GGG 0.453375 5.1:-18005341 MS.gene72872:CDS
AGTGGCAAGGTTGATGAAGC+TGG 0.461000 5.1:-18003938 MS.gene72872:CDS
AATCAACATTAGGGAAAGCT+TGG 0.471169 5.1:+18005108 None:intergenic
GCACTATCACTTACCCTCAT+TGG 0.472078 5.1:-18006126 MS.gene72872:CDS
TGTTGTTCCAATGATGTTGT+CGG 0.475822 5.1:-18005237 MS.gene72872:CDS
CTTTGACTTGCCGCAAAGGC+CGG 0.478751 5.1:-18005570 MS.gene72872:CDS
ATTTCGCAAACACTACTAAG+AGG 0.480566 5.1:+18005791 None:intergenic
TTTCACAACTATTCCTCACT+AGG 0.480714 5.1:-18004696 MS.gene72872:CDS
AAGATACCGAGATCTGCAAC+TGG 0.482468 5.1:-18003661 MS.gene72872:CDS
AAGCTCACCGCTGAGGCCTG+GGG 0.483974 5.1:+18004614 None:intergenic
TTCTTGACCACAAATGCCAC+TGG 0.484108 5.1:+18003908 None:intergenic
CCCAGATGAGGAATCAATAA+GGG 0.484993 5.1:+18005988 None:intergenic
CGATAAAAGGGTCGGTCGGA+AGG 0.485565 5.1:+18006056 None:intergenic
ATTTGATAATGTCTTTCAAA+CGG 0.487340 5.1:+18004240 None:intergenic
TCATAGAAATCTTATTGCTA+TGG 0.487900 5.1:-18005471 MS.gene72872:CDS
GTTAACTTTAGTCAAATCCT+TGG 0.487963 5.1:-18005942 MS.gene72872:CDS
GGGTCGGTCGGAAGGTTCCT+AGG 0.489518 5.1:+18006064 None:intergenic
AACAAAGCTTCTAAATCAGC+AGG 0.490080 5.1:+18004088 None:intergenic
GGTTTATCACTCTTTGCTAC+CGG 0.490982 5.1:-18005356 MS.gene72872:CDS
ACAGCAGTTTACAATCTCCT+AGG 0.491169 5.1:-18006081 MS.gene72872:CDS
AGCGGTGAGCTTTGGTTGAG+AGG 0.492979 5.1:-18004603 MS.gene72872:CDS
TTGCTCCATACAAGAAGGTT+CGG 0.499371 5.1:-18003702 MS.gene72872:CDS
CTATTGAGTGTTATGTATCT+AGG 0.499995 5.1:-18005836 MS.gene72872:CDS
AAAGTGGTAGATTGGAAAAG+TGG 0.504015 5.1:-18004648 MS.gene72872:CDS
TGTTTAATAACCGGCCTTTG+CGG 0.505033 5.1:+18005560 None:intergenic
ATCAAGAAGTTAGAGTCATT+AGG 0.508091 5.1:-18005713 MS.gene72872:CDS
GGTTATGGAATGACTGAGTC+GGG 0.510519 5.1:-18004753 MS.gene72872:CDS
GCGGTGAGCTTTGGTTGAGA+GGG 0.514715 5.1:-18004602 MS.gene72872:CDS
AGGAAACAACATCGATAAAA+GGG 0.516631 5.1:+18006044 None:intergenic
ACACTACTAAGAGGATTCAA+TGG 0.516632 5.1:+18005800 None:intergenic
GGGTAAGTGATAGTGCTGTT+GGG 0.518012 5.1:+18006133 None:intergenic
GAAAGACATTATCAAATACA+AGG 0.519394 5.1:-18004234 MS.gene72872:CDS
TTCATATACTTACGCTGTAA+CGG 0.523614 5.1:+18004036 None:intergenic
AACAGCTAGATACACAGTCT+TGG 0.524532 5.1:+18005400 None:intergenic
AGGGTAAGTGATAGTGCTGT+TGG 0.525684 5.1:+18006132 None:intergenic
TTTATAGCTCCTTTGCTCAA+AGG 0.526141 5.1:+18005140 None:intergenic
ATGTCGACAATTGATAAAGA+TGG 0.526979 5.1:-18004323 MS.gene72872:CDS
GTGGCAAGGTTGATGAAGCT+GGG 0.529856 5.1:-18003937 MS.gene72872:CDS
AAATCCTCCGAAAGCAACTC+AGG 0.535730 5.1:-18003636 MS.gene72872:CDS
TCCAAACATGGAGGCAAAAG+TGG 0.536470 5.1:-18004664 MS.gene72872:CDS
CATAAAATGGGTGTTTCACA+AGG 0.537350 5.1:-18005908 MS.gene72872:CDS
TTCACAACTATTCCTCACTA+GGG 0.540578 5.1:-18004695 MS.gene72872:CDS
GGGTTATGGAATGACTGAGT+CGG 0.541532 5.1:-18004754 MS.gene72872:CDS
GGTGCTGAGCCTTTGAGCAA+AGG 0.542374 5.1:-18005149 MS.gene72872:CDS
ATACAAGAAGGTTCGGAAAG+TGG 0.543810 5.1:-18003695 MS.gene72872:CDS
GTTCTACAGTCGTTGTAATG+AGG 0.550305 5.1:-18005319 MS.gene72872:CDS
ATGACTGAGTCGGGCGCAGT+AGG 0.553182 5.1:-18004744 MS.gene72872:CDS
AGAGGGCCTTCAATTATGAA+AGG 0.572900 5.1:-18004585 MS.gene72872:intron
GTGACTTTGACTTGCCGCAA+AGG 0.573724 5.1:-18005574 MS.gene72872:CDS
CGACAATTGATAAAGATGGT+TGG 0.573781 5.1:-18004319 MS.gene72872:CDS
AGGGCTAAAACCCCGTCATG+AGG 0.582643 5.1:+18006007 None:intergenic
AGCTCACCGCTGAGGCCTGG+GGG 0.583783 5.1:+18004615 None:intergenic
AAAGACATTATCAAATACAA+GGG 0.586533 5.1:-18004233 MS.gene72872:CDS
AAACCATATAAATGGAACAT+CGG 0.588130 5.1:+18005374 None:intergenic
CGTAGGATACTTGAATAATG+AGG 0.588911 5.1:-18004354 MS.gene72872:intron
AATTCCTGAGTTGCTTTCGG+AGG 0.590581 5.1:+18003632 None:intergenic
TTTATGAAGACCAGAAGCCA+AGG 0.591784 5.1:+18005925 None:intergenic
GCATTTGTGGTCAAGAAAGT+CGG 0.593489 5.1:-18003902 MS.gene72872:CDS
ATATACTTACGCTGTAACGG+CGG 0.594057 5.1:+18004039 None:intergenic
GGTAAGTGATAGTGCTGTTG+GGG 0.602978 5.1:+18006134 None:intergenic
GGCATTGACAGCGAAGGCAA+AGG 0.603341 5.1:-18005216 MS.gene72872:CDS
AGTTTAAGACATGTCTCTTG+CGG 0.607812 5.1:-18005170 MS.gene72872:CDS
AATAGAATTTACCATAAACA+AGG 0.609385 5.1:+18005621 None:intergenic
AGAACCCAATGACAACAATC+CGG 0.610232 5.1:+18005337 None:intergenic
CGTAAGCAAGTTAAGGAGTG+TGG 0.611437 5.1:-18005767 MS.gene72872:CDS
AGGGTTGTTAGCTCCAAACA+TGG 0.614345 5.1:-18004676 MS.gene72872:CDS
TTGACAGCGAAGGCAAAGGA+TGG 0.620619 5.1:-18005212 MS.gene72872:CDS
ATTAAACAAGAAGACACTGC+TGG 0.621260 5.1:-18005545 MS.gene72872:CDS
GCAGGTTGCTCCATACAAGA+AGG 0.622353 5.1:-18003707 MS.gene72872:intron
GGCACTACAGGAGTGAGCAA+AGG 0.626839 5.1:-18005506 MS.gene72872:CDS
GGGCTAAAACCCCGTCATGA+GGG 0.628723 5.1:+18006008 None:intergenic
AACAACATCGATAAAAGGGT+CGG 0.639732 5.1:+18006048 None:intergenic
AGAAATGGAAGTCCCAGATG+AGG 0.641379 5.1:+18005976 None:intergenic
TGGCAAGGTTGATGAAGCTG+GGG 0.643596 5.1:-18003936 MS.gene72872:CDS
ACATCGATAAAAGGGTCGGT+CGG 0.644565 5.1:+18006052 None:intergenic
TGGAGCTAACAACCCTAGTG+AGG 0.644923 5.1:+18004683 None:intergenic
AGATACCGAGATCTGCAACT+GGG 0.658351 5.1:-18003660 MS.gene72872:CDS
GTTGTCGGCATTGACAGCGA+AGG 0.659388 5.1:-18005222 MS.gene72872:CDS
TGCTTGACTTCTAAGCTCTG+AGG 0.664251 5.1:-18003610 None:intergenic
TCAACCAAAGCTCACCGCTG+AGG 0.681517 5.1:+18004607 None:intergenic
TCGGGCGCAGTAGGAACACG+CGG 0.689165 5.1:-18004735 MS.gene72872:CDS
TGAAGCTGGGGAGATTCCAG+TGG 0.700734 5.1:-18003924 MS.gene72872:CDS
GAGGAAATATGACATCGATG+AGG 0.723211 5.1:-18005300 MS.gene72872:CDS
GTTGTTAGCTCCAAACATGG+AGG 0.741487 5.1:-18004673 MS.gene72872:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATCTGTATTTTTATA+AGG - chr5.1:18005214-18005233 MS.gene72872:CDS 10.0%
!!! TTAAACTTTTTTTTTTTTTC+TGG - chr5.1:18006046-18006065 MS.gene72872:CDS 10.0%
!!! ATGAAATAAAGGAAAAATTT+TGG + chr5.1:18005397-18005416 None:intergenic 15.0%
!! AAAGACATTATCAAATACAA+GGG - chr5.1:18005542-18005561 MS.gene72872:CDS 20.0%
!! AATAGAATTTACCATAAACA+AGG + chr5.1:18004157-18004176 None:intergenic 20.0%
!! ATAGGATAGTAAATTGAATT+AGG + chr5.1:18003915-18003934 None:intergenic 20.0%
!! TAACAAATTTGAGTAAGAAA+TGG + chr5.1:18003817-18003836 None:intergenic 20.0%
!! TGGTAAATTCTATTCAATAA+AGG - chr5.1:18004163-18004182 MS.gene72872:intron 20.0%
!!! ATTGTTTATTTTGATCAAGA+TGG - chr5.1:18005494-18005513 MS.gene72872:CDS 20.0%
!!! ATTTGATAATGTCTTTCAAA+CGG + chr5.1:18005538-18005557 None:intergenic 20.0%
!!! TGTATGATTGTTTCTTTTTA+GGG - chr5.1:18005001-18005020 MS.gene72872:intron 20.0%
!!! TGTTTTTATAACCTTGTTTA+TGG - chr5.1:18004143-18004162 MS.gene72872:intron 20.0%
!!! TTTTGGTTAAGAATTTCAAA+TGG + chr5.1:18005380-18005399 None:intergenic 20.0%
!!! TTTTTACCTTTCATAATTGA+AGG + chr5.1:18005199-18005218 None:intergenic 20.0%
! AAACCATATAAATGGAACAT+CGG + chr5.1:18004404-18004423 None:intergenic 25.0%
! ACCTGTATAAAATCAACATT+AGG + chr5.1:18004680-18004699 None:intergenic 25.0%
! AGAGTGATAAACCATATAAA+TGG + chr5.1:18004412-18004431 None:intergenic 25.0%
! AGTTGAACTTTACTTGTTTA+TGG - chr5.1:18004939-18004958 MS.gene72872:intron 25.0%
! ATAAAGGAAAACCTTATTCA+TGG - chr5.1:18004179-18004198 MS.gene72872:intron 25.0%
! ATAACACTCAATAGTACAAT+AGG + chr5.1:18003933-18003952 None:intergenic 25.0%
! CAAGTAAAGTTCAACTATTA+TGG + chr5.1:18004935-18004954 None:intergenic 25.0%
! CCTGTATAAAATCAACATTA+GGG + chr5.1:18004679-18004698 None:intergenic 25.0%
! GAAAGACATTATCAAATACA+AGG - chr5.1:18005541-18005560 MS.gene72872:CDS 25.0%
! GATGGATATTTATATTTGTC+TGG - chr5.1:18005512-18005531 MS.gene72872:CDS 25.0%
! TCATAGAAATCTTATTGCTA+TGG - chr5.1:18004304-18004323 MS.gene72872:CDS 25.0%
! TGTGAATATGAATACCATAA+CGG + chr5.1:18004900-18004919 None:intergenic 25.0%
!! CTAATTTTCTTTTTTCTCCA+AGG + chr5.1:18004708-18004727 None:intergenic 25.0%
!! TCTAACTTCTTGATATTTTC+AGG + chr5.1:18004056-18004075 None:intergenic 25.0%
!! TTCAAATCCATTTCATTTTC+TGG + chr5.1:18004107-18004126 None:intergenic 25.0%
!! TTTTATACAGGTAAAAACCT+TGG - chr5.1:18004688-18004707 MS.gene72872:CDS 25.0%
!!! ACTTTTTTTTTTTTTCTGGC+AGG - chr5.1:18006050-18006069 MS.gene72872:CDS 25.0%
!!! ATTGTTTCTTTTTAGGGTTA+TGG - chr5.1:18005007-18005026 MS.gene72872:intron 25.0%
!!! CTGTATGATTGTTTCTTTTT+AGG - chr5.1:18005000-18005019 MS.gene72872:intron 25.0%
!!! GTTGATTTCCTAGATTTTTA+AGG - chr5.1:18004969-18004988 MS.gene72872:intron 25.0%
!!! TCATATTTTAATGTCCAAAG+AGG + chr5.1:18005789-18005808 None:intergenic 25.0%
!!! TTTTCTTTGTAATTGCTGTT+AGG - chr5.1:18004783-18004802 MS.gene72872:intron 25.0%
AAGGAAACAACATCGATAAA+AGG + chr5.1:18003735-18003754 None:intergenic 30.0%
AATCAATCTCATGCAAATGT+AGG + chr5.1:18005265-18005284 None:intergenic 30.0%
AGGAAACAACATCGATAAAA+GGG + chr5.1:18003734-18003753 None:intergenic 30.0%
AGTGTCTATTACTATTGGAA+TGG - chr5.1:18006013-18006032 MS.gene72872:CDS 30.0%
ATCAAGAAGTTAGAGTCATT+AGG - chr5.1:18004062-18004081 MS.gene72872:CDS 30.0%
ATGTCGACAATTGATAAAGA+TGG - chr5.1:18005452-18005471 MS.gene72872:CDS 30.0%
CTATTGAGTGTTATGTATCT+AGG - chr5.1:18003939-18003958 MS.gene72872:CDS 30.0%
CTTCACATCCTTAAAAATCT+AGG + chr5.1:18004980-18004999 None:intergenic 30.0%
GCTGGTATATTATATTCTTC+TGG - chr5.1:18004248-18004267 MS.gene72872:CDS 30.0%
GTCATAGTGTCTATTACTAT+TGG - chr5.1:18006008-18006027 MS.gene72872:CDS 30.0%
GTTAACTTTAGTCAAATCCT+TGG - chr5.1:18003833-18003852 MS.gene72872:intron 30.0%
TCCTACGAAGTATGAAATAA+AGG + chr5.1:18005408-18005427 None:intergenic 30.0%
TTCATATACTTACGCTGTAA+CGG + chr5.1:18005742-18005761 None:intergenic 30.0%
TTCATATTCACAACATGTTC+TGG - chr5.1:18004908-18004927 MS.gene72872:intron 30.0%
! GTGTCTTCTTGTTTAATAAC+CGG + chr5.1:18004227-18004246 None:intergenic 30.0%
! TCCTTTATTTCATACTTCGT+AGG - chr5.1:18005404-18005423 MS.gene72872:CDS 30.0%
! TGACTTGCGTTCTTTAATTA+AGG + chr5.1:18004731-18004750 None:intergenic 30.0%
!! TTTTTGGTGAATACCAATGA+GGG + chr5.1:18003665-18003684 None:intergenic 30.0%
!!! CCCTAATGTTGATTTTATAC+AGG - chr5.1:18004676-18004695 MS.gene72872:CDS 30.0%
AAAGTGGTAGATTGGAAAAG+TGG - chr5.1:18005127-18005146 MS.gene72872:CDS 35.0%
AACAAAGCTTCTAAATCAGC+AGG + chr5.1:18005690-18005709 None:intergenic 35.0%
AACAACATCGATAAAAGGGT+CGG + chr5.1:18003730-18003749 None:intergenic 35.0%
AATCAACATTAGGGAAAGCT+TGG + chr5.1:18004670-18004689 None:intergenic 35.0%
AATGGAATAGCAAAAGAGAC+AGG + chr5.1:18005362-18005381 None:intergenic 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr5.1:18003978-18003997 None:intergenic 35.0%
AGTCAAAGTCACCATGAATA+AGG + chr5.1:18004193-18004212 None:intergenic 35.0%
ATGAGGGTGAGAAAAAATGA+AGG + chr5.1:18003754-18003773 None:intergenic 35.0%
ATTAAACAAGAAGACACTGC+TGG - chr5.1:18004230-18004249 MS.gene72872:CDS 35.0%
ATTTCGCAAACACTACTAAG+AGG + chr5.1:18003987-18004006 None:intergenic 35.0%
CAAAAGAGACAGGCAAAAAA+AGG + chr5.1:18005352-18005371 None:intergenic 35.0%
CATAAAATGGGTGTTTCACA+AGG - chr5.1:18003867-18003886 MS.gene72872:CDS 35.0%
CATTGGTATTCACCAAAAAC+TGG - chr5.1:18003666-18003685 MS.gene72872:CDS 35.0%
CGAAAATGATGTGATACTCA+AGG + chr5.1:18005812-18005831 None:intergenic 35.0%
CGAAATTCGTAAGCAAGTTA+AGG - chr5.1:18004001-18004020 MS.gene72872:intron 35.0%
CGACAATTGATAAAGATGGT+TGG - chr5.1:18005456-18005475 MS.gene72872:CDS 35.0%
CGTAGGATACTTGAATAATG+AGG - chr5.1:18005421-18005440 MS.gene72872:CDS 35.0%
GTTAGGAGCTAAATAAATGC+TGG - chr5.1:18004800-18004819 MS.gene72872:intron 35.0%
TATCAAATACAAGGGCTTTC+AGG - chr5.1:18005550-18005569 MS.gene72872:CDS 35.0%
TGTTGTTCCAATGATGTTGT+CGG - chr5.1:18004538-18004557 MS.gene72872:intron 35.0%
TTATATTCTTCTGGCACTAC+AGG - chr5.1:18004257-18004276 MS.gene72872:CDS 35.0%
TTCACAACTATTCCTCACTA+GGG - chr5.1:18005080-18005099 MS.gene72872:intron 35.0%
TTGCCGATGTTCCATTTATA+TGG - chr5.1:18004398-18004417 MS.gene72872:intron 35.0%
TTTATAGCTCCTTTGCTCAA+AGG + chr5.1:18004638-18004657 None:intergenic 35.0%
TTTCACAACTATTCCTCACT+AGG - chr5.1:18005079-18005098 MS.gene72872:intron 35.0%
! AAAAAGGTACAAGTAGGCTA+AGG + chr5.1:18005336-18005355 None:intergenic 35.0%
! AGGCAAAAAAAGGTACAAGT+AGG + chr5.1:18005342-18005361 None:intergenic 35.0%
! AGTTTAAGACATGTCTCTTG+CGG - chr5.1:18004605-18004624 MS.gene72872:CDS 35.0%
! CACATCATTTTCGATATCAG+TGG - chr5.1:18005819-18005838 MS.gene72872:CDS 35.0%
! CTGCTGTAAATTCCAGTTTT+TGG + chr5.1:18003681-18003700 None:intergenic 35.0%
! GCTTCTGGTCTTCATAAAAT+GGG - chr5.1:18003855-18003874 MS.gene72872:intron 35.0%
!!! ATTTTTTCTCACCCTCATGA+CGG - chr5.1:18003756-18003775 MS.gene72872:intron 35.0%
!!! GTTTTTGGTGAATACCAATG+AGG + chr5.1:18003666-18003685 None:intergenic 35.0%
AACAGCTAGATACACAGTCT+TGG + chr5.1:18004378-18004397 None:intergenic 40.0%
ACAGCAGTTTACAATCTCCT+AGG - chr5.1:18003694-18003713 MS.gene72872:CDS 40.0%
ACTTTCCGAACCTTCTTGTA+TGG + chr5.1:18006081-18006100 None:intergenic 40.0%
AGAACCCAATGACAACAATC+CGG + chr5.1:18004441-18004460 None:intergenic 40.0%
AGAGGGCCTTCAATTATGAA+AGG - chr5.1:18005190-18005209 MS.gene72872:CDS 40.0%
ATACAAGAAGGTTCGGAAAG+TGG - chr5.1:18006080-18006099 MS.gene72872:CDS 40.0%
ATATACTTACGCTGTAACGG+CGG + chr5.1:18005739-18005758 None:intergenic 40.0%
ATGACATCGATGAGGCTATT+AGG - chr5.1:18004483-18004502 MS.gene72872:intron 40.0%
CCCAGATGAGGAATCAATAA+GGG + chr5.1:18003790-18003809 None:intergenic 40.0%
GAGGAAATATGACATCGATG+AGG - chr5.1:18004475-18004494 MS.gene72872:intron 40.0%
GCATTTGTGGTCAAGAAAGT+CGG - chr5.1:18005873-18005892 MS.gene72872:CDS 40.0%
GGTTTATCACTCTTTGCTAC+CGG - chr5.1:18004419-18004438 MS.gene72872:intron 40.0%
GTTCTACAGTCGTTGTAATG+AGG - chr5.1:18004456-18004475 MS.gene72872:intron 40.0%
TCCCAGATGAGGAATCAATA+AGG + chr5.1:18003791-18003810 None:intergenic 40.0%
TGACATCGATGAGGCTATTA+GGG - chr5.1:18004484-18004503 MS.gene72872:intron 40.0%
TGCTGTGCCAGAAAATGAAA+TGG - chr5.1:18004097-18004116 MS.gene72872:CDS 40.0%
TGTTTAATAACCGGCCTTTG+CGG + chr5.1:18004218-18004237 None:intergenic 40.0%
TTAGTCAAATCCTTGGCTTC+TGG - chr5.1:18003840-18003859 MS.gene72872:intron 40.0%
TTGCTCCATACAAGAAGGTT+CGG - chr5.1:18006073-18006092 MS.gene72872:CDS 40.0%
TTTATGAAGACCAGAAGCCA+AGG + chr5.1:18003853-18003872 None:intergenic 40.0%
! AAAGATGGTTGGCTACATAC+TGG - chr5.1:18005467-18005486 MS.gene72872:CDS 40.0%
! ACACTATTTCTTCGCCGTTA+TGG - chr5.1:18004883-18004902 MS.gene72872:intron 40.0%
! AGCAATTCCTGAGTTGCTTT+CGG + chr5.1:18006149-18006168 None:intergenic 40.0%
! ATTTTCCCAGTTGCAGATCT+CGG + chr5.1:18006123-18006142 None:intergenic 40.0%
! CATTTTCGATATCAGTGGCA+AGG - chr5.1:18005824-18005843 MS.gene72872:CDS 40.0%
! GGCTTCTGGTCTTCATAAAA+TGG - chr5.1:18003854-18003873 MS.gene72872:intron 40.0%
!! CCCTTATTGATTCCTCATCT+GGG - chr5.1:18003787-18003806 MS.gene72872:intron 40.0%
!! TGTCTTCTAAAGTGCCTCTT+TGG - chr5.1:18005772-18005791 MS.gene72872:CDS 40.0%
!! TTTTCTGGCACAGCAATAAC+AGG + chr5.1:18004092-18004111 None:intergenic 40.0%
!!! TTTTTTCTCACCCTCATGAC+GGG - chr5.1:18003757-18003776 MS.gene72872:intron 40.0%
AAATCCTCCGAAAGCAACTC+AGG - chr5.1:18006139-18006158 MS.gene72872:CDS 45.0%
AAGATACCGAGATCTGCAAC+TGG - chr5.1:18006114-18006133 MS.gene72872:CDS 45.0%
ACATCGATAAAAGGGTCGGT+CGG + chr5.1:18003726-18003745 None:intergenic 45.0%
AGAAATGGAAGTCCCAGATG+AGG + chr5.1:18003802-18003821 None:intergenic 45.0%
AGATACCGAGATCTGCAACT+GGG - chr5.1:18006115-18006134 MS.gene72872:CDS 45.0%
AGGGTTGTTAGCTCCAAACA+TGG - chr5.1:18005099-18005118 MS.gene72872:intron 45.0%
CGTAAGCAAGTTAAGGAGTG+TGG - chr5.1:18004008-18004027 MS.gene72872:intron 45.0%
GATCGATTACGTTGCTAAGC+AGG - chr5.1:18005920-18005939 MS.gene72872:CDS 45.0%
GCACTATCACTTACCCTCAT+TGG - chr5.1:18003649-18003668 MS.gene72872:CDS 45.0%
GCTACCGGATTGTTGTCATT+GGG - chr5.1:18004434-18004453 MS.gene72872:intron 45.0%
GGGTTATGGAATGACTGAGT+CGG - chr5.1:18005021-18005040 MS.gene72872:intron 45.0%
GGTTATGGAATGACTGAGTC+GGG - chr5.1:18005022-18005041 MS.gene72872:intron 45.0%
GTCAATGCCGACAACATCAT+TGG + chr5.1:18004548-18004567 None:intergenic 45.0%
GTTGTTAGCTCCAAACATGG+AGG - chr5.1:18005102-18005121 MS.gene72872:CDS 45.0%
TCCAAACATGGAGGCAAAAG+TGG - chr5.1:18005111-18005130 MS.gene72872:CDS 45.0%
TGCTACCGGATTGTTGTCAT+TGG - chr5.1:18004433-18004452 MS.gene72872:intron 45.0%
TGGAGGCAAAAGTGGTAGAT+TGG - chr5.1:18005119-18005138 MS.gene72872:CDS 45.0%
TTCTTGACCACAAATGCCAC+TGG + chr5.1:18005870-18005889 None:intergenic 45.0%
! AATTCCTGAGTTGCTTTCGG+AGG + chr5.1:18006146-18006165 None:intergenic 45.0%
! AGGGTAAGTGATAGTGCTGT+TGG + chr5.1:18003646-18003665 None:intergenic 45.0%
! GGGTAAGTGATAGTGCTGTT+GGG + chr5.1:18003645-18003664 None:intergenic 45.0%
! GGTAAGTGATAGTGCTGTTG+GGG + chr5.1:18003644-18003663 None:intergenic 45.0%
! TTTTTCTCACCCTCATGACG+GGG - chr5.1:18003758-18003777 MS.gene72872:intron 45.0%
!! ACCACTTTTGCCTCCATGTT+TGG + chr5.1:18005115-18005134 None:intergenic 45.0%
!! GCCCTTATTGATTCCTCATC+TGG - chr5.1:18003786-18003805 MS.gene72872:intron 45.0%
ACGGCGGCATCAACTATTTC+AGG + chr5.1:18005723-18005742 None:intergenic 50.0%
GCAGGTTGCTCCATACAAGA+AGG - chr5.1:18006068-18006087 MS.gene72872:CDS 50.0%
GGAGATTCCAGTGGCATTTG+TGG - chr5.1:18005860-18005879 MS.gene72872:CDS 50.0%
TGGAGCTAACAACCCTAGTG+AGG + chr5.1:18005095-18005114 None:intergenic 50.0%
TTGACAGCGAAGGCAAAGGA+TGG - chr5.1:18004563-18004582 MS.gene72872:intron 50.0%
! AGTGGTAAGTTTTTGCCCCC+AGG - chr5.1:18005145-18005164 MS.gene72872:CDS 50.0%
!! AGTGGCAAGGTTGATGAAGC+TGG - chr5.1:18005837-18005856 MS.gene72872:CDS 50.0%
!! GTGACTTTGACTTGCCGCAA+AGG - chr5.1:18004201-18004220 MS.gene72872:intron 50.0%
!! GTGGCAAGGTTGATGAAGCT+GGG - chr5.1:18005838-18005857 MS.gene72872:CDS 50.0%
!! TGGCAAGGTTGATGAAGCTG+GGG - chr5.1:18005839-18005858 MS.gene72872:CDS 50.0%
AGGGCTAAAACCCCGTCATG+AGG + chr5.1:18003771-18003790 None:intergenic 55.0%
CGATAAAAGGGTCGGTCGGA+AGG + chr5.1:18003722-18003741 None:intergenic 55.0%
GGCACTACAGGAGTGAGCAA+AGG - chr5.1:18004269-18004288 MS.gene72872:CDS 55.0%
GGGCTAAAACCCCGTCATGA+GGG + chr5.1:18003770-18003789 None:intergenic 55.0%
TCAACCAAAGCTCACCGCTG+AGG + chr5.1:18005171-18005190 None:intergenic 55.0%
TGAAGCTGGGGAGATTCCAG+TGG - chr5.1:18005851-18005870 MS.gene72872:CDS 55.0%
! AGCGGTGAGCTTTGGTTGAG+AGG - chr5.1:18005172-18005191 MS.gene72872:CDS 55.0%
! GCGGTGAGCTTTGGTTGAGA+GGG - chr5.1:18005173-18005192 MS.gene72872:CDS 55.0%
! GGTGCTGAGCCTTTGAGCAA+AGG - chr5.1:18004626-18004645 MS.gene72872:CDS 55.0%
!! CTTTGACTTGCCGCAAAGGC+CGG - chr5.1:18004205-18004224 MS.gene72872:intron 55.0%
!! GGCATTGACAGCGAAGGCAA+AGG - chr5.1:18004559-18004578 MS.gene72872:intron 55.0%
!! GTTGTCGGCATTGACAGCGA+AGG - chr5.1:18004553-18004572 MS.gene72872:intron 55.0%
AAAGCTCACCGCTGAGGCCT+GGG + chr5.1:18005165-18005184 None:intergenic 60.0%
ATGACTGAGTCGGGCGCAGT+AGG - chr5.1:18005031-18005050 MS.gene72872:intron 60.0%
! TTTTTGCCCCCAGGCCTCAG+CGG - chr5.1:18005154-18005173 MS.gene72872:CDS 60.0%
AAGCTCACCGCTGAGGCCTG+GGG + chr5.1:18005164-18005183 None:intergenic 65.0%
CAAAGCTCACCGCTGAGGCC+TGG + chr5.1:18005166-18005185 None:intergenic 65.0%
CAGGCCTCAGCGGTGAGCTT+TGG - chr5.1:18005164-18005183 MS.gene72872:CDS 65.0%
GGGTCGGTCGGAAGGTTCCT+AGG + chr5.1:18003714-18003733 None:intergenic 65.0%
TCGGGCGCAGTAGGAACACG+CGG - chr5.1:18005040-18005059 MS.gene72872:intron 65.0%
AGCTCACCGCTGAGGCCTGG+GGG + chr5.1:18005163-18005182 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 18003612 18006185 18003612 ID=MS.gene72872
chr5.1 mRNA 18003612 18006185 18003612 ID=MS.gene72872.t1;Parent=MS.gene72872
chr5.1 exon 18005100 18006185 18005100 ID=MS.gene72872.t1.exon1;Parent=MS.gene72872.t1
chr5.1 CDS 18005100 18006185 18005100 ID=cds.MS.gene72872.t1;Parent=MS.gene72872.t1
chr5.1 exon 18004586 18004775 18004586 ID=MS.gene72872.t1.exon2;Parent=MS.gene72872.t1
chr5.1 CDS 18004586 18004775 18004586 ID=cds.MS.gene72872.t1;Parent=MS.gene72872.t1
chr5.1 exon 18004226 18004371 18004226 ID=MS.gene72872.t1.exon3;Parent=MS.gene72872.t1
chr5.1 CDS 18004226 18004371 18004226 ID=cds.MS.gene72872.t1;Parent=MS.gene72872.t1
chr5.1 exon 18004049 18004116 18004049 ID=MS.gene72872.t1.exon4;Parent=MS.gene72872.t1
chr5.1 CDS 18004049 18004116 18004049 ID=cds.MS.gene72872.t1;Parent=MS.gene72872.t1
chr5.1 exon 18003856 18003958 18003856 ID=MS.gene72872.t1.exon5;Parent=MS.gene72872.t1
chr5.1 CDS 18003856 18003958 18003856 ID=cds.MS.gene72872.t1;Parent=MS.gene72872.t1
chr5.1 exon 18003612 18003725 18003612 ID=MS.gene72872.t1.exon6;Parent=MS.gene72872.t1
chr5.1 CDS 18003612 18003725 18003612 ID=cds.MS.gene72872.t1;Parent=MS.gene72872.t1
Gene Sequence

>MS.gene72872

ATGGCTGCAAACTGGAATTTTTTCTCTCTCCCCAACAGCACTATCACTTACCCTCATTGGTATTCACCAAAAACTGGAATTTACAGCAGTTTACAATCTCCTAGGAACCTTCCGACCGACCCTTTTATCGATGTTGTTTCCTTCATTTTTTCTCACCCTCATGACGGGGTTTTAGCCCTTATTGATTCCTCATCTGGGACTTCCATTTCTTACTCAAATTTGTTAACTTTAGTCAAATCCTTGGCTTCTGGTCTTCATAAAATGGGTGTTTCACAAGGTGATGTTGTTTTGCTTTTACTTCCTAATTCAATTTACTATCCTATTGTACTATTGAGTGTTATGTATCTAGGTGCTGTTGTTACACCATTGAATCCTCTTAGTAGTGTTTGCGAAATTCGTAAGCAAGTTAAGGAGTGTGGTGTGAGCTTTGCTTTTACTATACCTGAAAATATCAAGAAGTTAGAGTCATTAGGCATTCCTGTTATTGCTGTGCCAGAAAATGAAATGGATTTGAAGCATGATTGTTTCTCATGTTTTTATAACCTTGTTTATGGTAAATTCTATTCAATAAAGGAAAACCTTATTCATGGTGACTTTGACTTGCCGCAAAGGCCGGTTATTAAACAAGAAGACACTGCTGGTATATTATATTCTTCTGGCACTACAGGAGTGAGCAAAGGAGTTGTTTTAACTCATAGAAATCTTATTGCTATGGTTGAGCTTTTTGTGAGATTTGAAGCTTCTCAATATGAATACTCTAGTTCCAAGACTGTGTATCTAGCTGTTTTGCCGATGTTCCATTTATATGGTTTATCACTCTTTGCTACCGGATTGTTGTCATTGGGTTCTACAGTCGTTGTAATGAGGAAATATGACATCGATGAGGCTATTAGGGCGATTGATAAATATAAAGTTACACACTTTCATGTTGTTCCAATGATGTTGTCGGCATTGACAGCGAAGGCAAAGGATGGTAATAGAAGTAAGTTGCAGAGTTTAAGACATGTCTCTTGCGGTGCTGAGCCTTTGAGCAAAGGAGCTATAAATGATTTTGTCCAAGCTTTCCCTAATGTTGATTTTATACAGGTAAAAACCTTGGAGAAAAAAGAAAATTAGCCTTAATTAAAGAACGCAAGTCATGCTACATCATTTATATAATTGATTGAGTTATTTTTCTTTGTAATTGCTGTTAGGAGCTAAATAAATGCTGGTTTCATTTAGTTATGAGAGAACTTGTGAACTCTAAAATAATTGTTAATAGCAGATGCTAAACACTATTTCTTCGCCGTTATGGTATTCATATTCACAACATGTTCTGGACCATAATAGTTGAACTTTACTTGTTTATGGTAAAAATGTTGATTTCCTAGATTTTTAAGGATGTGAAGCTGTATGATTGTTTCTTTTTAGGGTTATGGAATGACTGAGTCGGGCGCAGTAGGAACACGCGGCTTCAATACTGATAAGTTTCACAACTATTCCTCACTAGGGTTGTTAGCTCCAAACATGGAGGCAAAAGTGGTAGATTGGAAAAGTGGTAAGTTTTTGCCCCCAGGCCTCAGCGGTGAGCTTTGGTTGAGAGGGCCTTCAATTATGAAAGGTAAAAAATCTGTATTTTTATAAGGTCTTGTCTATTGTGTGTAATCTTAACCTACATTTGCATGAGATTGATTCAATGATTTCAACTGCTGTGTTGCTATAACTTTTGTTTCTGTCTTATTCCTTAGCCTACTTGTACCTTTTTTTGCCTGTCTCTTTTGCTATTCCATTTGAAATTCTTAACCAAAATTTTTCCTTTATTTCATACTTCGTAGGATACTTGAATAATGAGGAAGCTACAATGTCGACAATTGATAAAGATGGTTGGCTACATACTGGAGATATTGTTTATTTTGATCAAGATGGATATTTATATTTGTCTGGCCGTTTGAAAGACATTATCAAATACAAGGGCTTTCAGGTATCAAACTTTTTGTTTATTAAACTTCTATAAGAAAATGTTCTTATACAATTTATTTTTACTAAATATGTATGCATTGTTCTTATGTATGACACATGATCTTTTACAGATTGCTCCTGCTGATTTAGAAGCTTTGTTAATTTCGCATCCTGAAATAGTTGATGCCGCCGTTACAGCGTAAGTATATGAACACTTCATTTTGTCTTCTAAAGTGCCTCTTTGGACATTAAAATATGACCTTGAGTATCACATCATTTTCGATATCAGTGGCAAGGTTGATGAAGCTGGGGAGATTCCAGTGGCATTTGTGGTCAAGAAAGTCGGAAGTGTACTTTCTTCACAGCATGTGATCGATTACGTTGCTAAGCAGGTTTTTCTCTATGAGACAGACATGAAGTTCTAAGATATTTGAATCGATATGACTACTAGAGTCGATGTCATAGTGTCTATTACTATTGGAATGGTTTTCACATTTTAAACTTTTTTTTTTTTTCTGGCAGGTTGCTCCATACAAGAAGGTTCGGAAAGTGGTCTTCACAGACAAGATACCGAGATCTGCAACTGGGAAAATCCTCCGAAAGCAACTCAGGAATTGCTTGACTTCTAAGCTCTGA
Protein sequence

>MS.gene72872.t1

MAANWNFFSLPNSTITYPHWYSPKTGIYSSLQSPRNLPTDPFIDVVSFIFSHPHDGVLALIDSSSGTSISYSNLLTLVKSLASGLHKMGVSQGDVVLLLLPNSIYYPIVLLSVMYLGAVVTPLNPLSSVCEIRKQVKECGVSFAFTIPENIKKLESLGIPVIAVPENEMDLKHDCFSCFYNLVYGKFYSIKENLIHGDFDLPQRPVIKQEDTAGILYSSGTTGVSKGVVLTHRNLIAMVELFVRFEASQYEYSSSKTVYLAVLPMFHLYGLSLFATGLLSLGSTVVVMRKYDIDEAIRAIDKYKVTHFHVVPMMLSALTAKAKDGNRSKLQSLRHVSCGAEPLSKGAINDFVQAFPNVDFIQGYGMTESGAVGTRGFNTDKFHNYSSLGLLAPNMEAKVVDWKSGKFLPPGLSGELWLRGPSIMKGYLNNEEATMSTIDKDGWLHTGDIVYFDQDGYLYLSGRLKDIIKYKGFQIAPADLEALLISHPEIVDAAVTAGKVDEAGEIPVAFVVKKVGSVLSSQHVIDYVAKQVAPYKKVRKVVFTDKIPRSATGKILRKQLRNCLTSKL