AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070120.t1 MTR_5g027480 94.054 555 33 0 1 555 1 555 0.0 1067
MS.gene070120.t1 MTR_5g027500 81.338 568 93 2 1 555 1 568 0.0 911
MS.gene070120.t1 MTR_4g077900 82.427 478 82 1 80 555 1 478 0.0 802
MS.gene070120.t1 MTR_1g026310 44.607 547 286 6 20 555 20 560 5.14e-156 457
MS.gene070120.t1 MTR_8g039720 43.739 567 295 9 5 555 1 559 3.32e-152 448
MS.gene070120.t1 MTR_1g090737 45.018 542 274 10 20 547 27 558 2.16e-145 431
MS.gene070120.t1 MTR_4g057730 41.791 536 297 8 20 547 6 534 2.60e-137 409
MS.gene070120.t1 MTR_2g095510 41.078 538 302 7 25 555 10 539 1.28e-135 405
MS.gene070120.t1 MTR_1g063120 40.437 549 296 9 20 547 6 544 1.41e-133 400
MS.gene070120.t1 MTR_3g088870 42.339 496 268 7 58 547 67 550 2.18e-130 392
MS.gene070120.t1 MTR_3g088880 42.308 494 268 8 58 547 68 548 2.63e-128 387
MS.gene070120.t1 MTR_1g063110 39.227 543 307 8 20 547 6 540 7.92e-127 382
MS.gene070120.t1 MTR_4g057730 41.189 471 262 8 20 482 6 469 6.73e-112 342
MS.gene070120.t1 MTR_2g105570 40.301 531 297 10 30 548 20 542 1.18e-111 343
MS.gene070120.t1 MTR_1g073180 39.768 518 283 8 42 549 30 528 1.08e-110 340
MS.gene070120.t1 MTR_4g128337 39.000 500 287 10 58 547 49 540 1.40e-107 333
MS.gene070120.t1 MTR_4g005750 40.454 529 293 11 30 548 19 535 3.72e-105 327
MS.gene070120.t1 MTR_4g057730 40.576 451 253 8 20 462 6 449 2.36e-103 319
MS.gene070120.t1 MTR_3g460770 35.502 538 329 8 26 555 14 541 2.57e-102 319
MS.gene070120.t1 MTR_5g007640 38.780 508 290 8 58 553 87 585 2.23e-101 318
MS.gene070120.t1 MTR_2g095510 39.775 445 253 7 25 462 10 446 2.52e-100 311
MS.gene070120.t1 MTR_1g073180 38.878 499 276 9 42 530 52 531 1.56e-99 311
MS.gene070120.t1 MTR_1g026470 38.660 388 221 6 20 396 20 401 2.53e-79 255
MS.gene070120.t1 MTR_1g026430 38.660 388 221 6 20 396 20 401 6.34e-79 254
MS.gene070120.t1 MTR_3g035130 30.801 487 315 11 68 545 32 505 1.04e-53 190
MS.gene070120.t1 MTR_5g076240 28.244 524 317 17 66 549 38 542 4.62e-44 165
MS.gene070120.t1 MTR_1569s0010 26.145 524 329 13 68 549 22 529 2.85e-43 162
MS.gene070120.t1 MTR_3g035130 30.612 441 284 11 68 499 32 459 1.59e-42 159
MS.gene070120.t1 MTR_0803s0020 27.539 512 291 14 70 541 263 734 5.10e-37 147
MS.gene070120.t1 MTR_4g011790 27.820 399 251 10 182 554 211 598 8.10e-37 144
MS.gene070120.t1 MTR_5g076220 29.810 369 231 9 196 548 186 542 1.29e-36 144
MS.gene070120.t1 MTR_2g044180 29.610 385 244 11 181 548 87 461 1.38e-36 142
MS.gene070120.t1 MTR_2g044180 27.183 504 321 14 81 548 59 552 1.44e-36 143
MS.gene070120.t1 MTR_3g064420 27.205 533 319 17 64 548 36 547 1.91e-36 143
MS.gene070120.t1 MTR_2g030240 26.433 541 318 15 71 555 31 547 6.13e-36 141
MS.gene070120.t1 MTR_5g008820 27.135 527 301 18 69 548 41 531 4.93e-35 139
MS.gene070120.t1 MTR_4g011790 25.820 488 308 14 109 554 132 607 5.59e-35 139
MS.gene070120.t1 MTR_0161s0050 28.610 374 229 11 196 548 188 544 1.18e-34 138
MS.gene070120.t1 MTR_4g013210 28.079 406 245 12 182 554 54 445 1.28e-34 136
MS.gene070120.t1 MTR_5g008800 28.487 509 321 15 69 549 41 534 1.41e-34 137
MS.gene070120.t1 MTR_4g121600 25.581 516 338 15 69 548 47 552 2.34e-34 137
MS.gene070120.t1 MTR_4g011790 27.649 387 243 10 182 542 211 586 8.83e-34 136
MS.gene070120.t1 MTR_8g074040 27.308 509 305 19 80 548 94 577 1.40e-33 135
MS.gene070120.t1 MTR_8g074040 28.871 381 236 13 185 549 99 460 9.68e-33 131
MS.gene070120.t1 MTR_5g076200 27.807 374 232 11 196 548 186 542 5.02e-32 130
MS.gene070120.t1 MTR_8g074120 28.649 370 229 13 198 549 137 489 1.21e-30 125
MS.gene070120.t1 MTR_2g030240 27.889 398 232 10 197 555 72 453 1.84e-30 124
MS.gene070120.t1 MTR_7g084850 24.034 595 386 18 6 555 151 724 2.75e-29 123
MS.gene070120.t1 MTR_4g013210 27.390 387 231 11 182 554 54 404 1.97e-27 114
MS.gene070120.t1 MTR_1g026350 41.045 134 79 0 20 153 20 153 2.44e-27 108
MS.gene070120.t1 MTR_4g013210 28.150 373 225 12 182 523 206 566 4.79e-27 115
MS.gene070120.t1 MTR_5g023730 27.027 518 322 21 69 553 48 542 5.48e-27 115
MS.gene070120.t1 MTR_4g013210 27.390 387 231 11 182 554 206 556 9.80e-27 114
MS.gene070120.t1 MTR_1g104810 24.858 527 304 16 23 472 57 568 1.67e-23 105
MS.gene070120.t1 MTR_1g104810 25.052 483 282 14 55 472 34 501 5.13e-22 100
MS.gene070120.t1 MTR_4g057755 55.556 72 32 0 411 482 46 117 7.35e-22 91.7
MS.gene070120.t1 MTR_3g087980 25.510 490 267 18 68 475 106 579 9.41e-22 99.8
MS.gene070120.t1 MTR_1g030290 28.286 350 171 16 198 480 250 586 1.20e-20 96.3
MS.gene070120.t1 MTR_1054s0020 25.770 357 240 11 200 549 432 770 2.16e-20 95.9
MS.gene070120.t1 MTR_1g086600 23.818 592 355 20 37 551 58 630 1.29e-17 87.0
MS.gene070120.t1 MTR_8g099420 26.131 398 216 14 140 484 180 552 1.36e-17 86.7
MS.gene070120.t1 MTR_7g109830 24.197 529 305 18 37 484 36 549 1.86e-17 86.3
MS.gene070120.t1 MTR_8g083170 31.282 195 116 5 351 532 482 671 8.12e-16 81.3
MS.gene070120.t1 MTR_4g066130 25.945 397 237 17 197 544 650 1038 1.57e-15 80.9
MS.gene070120.t1 MTR_2g098490 26.010 396 237 16 198 544 647 1035 2.01e-15 80.5
MS.gene070120.t1 MTR_3g084470 31.019 216 128 6 296 495 418 628 1.23e-14 77.4
MS.gene070120.t1 MTR_3g084450 31.313 198 113 7 315 495 233 424 3.77e-14 75.5
MS.gene070120.t1 MTR_3g088890 48.438 64 33 0 457 520 10 73 5.84e-14 68.2
MS.gene070120.t1 MTR_3g084450 31.313 198 113 7 315 495 441 632 1.37e-13 74.3
MS.gene070120.t1 MTR_4g013210 27.863 262 157 10 182 423 206 455 9.69e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070120.t1 AT4G19010 60.662 544 211 3 11 551 15 558 0.0 668
MS.gene070120.t1 AT1G20510 46.402 528 268 6 27 547 17 536 1.34e-163 476
MS.gene070120.t1 AT1G20480 43.144 547 292 6 20 555 27 565 2.29e-151 446
MS.gene070120.t1 AT1G20500 42.193 538 292 6 27 555 23 550 1.34e-144 428
MS.gene070120.t1 AT4G05160 41.068 543 304 6 23 555 8 544 1.04e-141 421
MS.gene070120.t1 AT1G20510 45.356 463 238 6 27 482 17 471 1.83e-136 405
MS.gene070120.t1 AT5G63380 40.884 543 297 7 20 547 17 550 6.11e-136 406
MS.gene070120.t1 AT5G38120 42.293 532 283 9 27 547 24 542 4.49e-135 404
MS.gene070120.t1 AT1G20510 44.470 443 231 6 27 462 17 451 1.46e-125 377
MS.gene070120.t1 AT5G38120 41.270 504 272 9 27 519 24 514 4.49e-121 367
MS.gene070120.t1 AT3G21240 41.011 534 298 8 30 555 30 554 8.58e-117 357
MS.gene070120.t1 AT1G65060 41.935 496 279 8 58 548 66 557 1.14e-110 341
MS.gene070120.t1 AT1G51680 42.323 508 281 6 58 555 56 561 2.36e-110 340
MS.gene070120.t1 AT5G38120 41.113 467 251 9 27 482 24 477 1.25e-109 336
MS.gene070120.t1 AT5G38120 41.113 467 251 9 27 482 24 477 1.25e-109 336
MS.gene070120.t1 AT3G21230 39.544 526 304 7 40 553 43 566 6.83e-109 337
MS.gene070120.t1 AT1G62940 38.583 508 281 9 57 549 43 534 2.78e-99 311
MS.gene070120.t1 AT1G51680 41.949 472 262 6 58 519 56 525 2.81e-98 308
MS.gene070120.t1 AT3G21230 39.228 492 285 7 40 519 43 532 2.05e-97 306
MS.gene070120.t1 AT3G21240 39.525 463 263 8 30 484 30 483 3.88e-91 288
MS.gene070120.t1 AT3G21240 39.525 463 263 8 30 484 30 483 3.88e-91 288
MS.gene070120.t1 AT1G20490 38.498 426 237 8 22 431 12 428 2.78e-89 282
MS.gene070120.t1 AT1G20490 38.498 426 237 8 22 431 12 428 2.78e-89 282
MS.gene070120.t1 AT3G21230 38.242 455 267 7 40 482 43 495 1.40e-84 271
MS.gene070120.t1 AT1G51680 40.275 437 249 6 58 484 56 490 4.14e-84 270
MS.gene070120.t1 AT1G65060 39.806 412 239 8 58 464 66 473 1.78e-78 256
MS.gene070120.t1 AT3G48990 29.630 486 324 12 67 545 30 504 7.78e-50 180
MS.gene070120.t1 AT3G21230 33.679 386 242 7 40 413 43 426 3.73e-49 176
MS.gene070120.t1 AT1G21530 27.505 549 334 21 41 549 19 543 5.71e-43 161
MS.gene070120.t1 AT1G77240 33.687 377 218 14 195 552 182 545 4.16e-42 159
MS.gene070120.t1 AT1G21530 27.422 547 335 21 41 549 19 541 9.23e-42 158
MS.gene070120.t1 AT1G21540 27.143 560 332 23 41 552 19 550 3.16e-40 154
MS.gene070120.t1 AT5G16370 28.032 503 313 17 80 549 53 539 2.69e-39 151
MS.gene070120.t1 AT1G65890 25.940 532 327 16 66 549 38 550 4.42e-39 151
MS.gene070120.t1 AT1G30520 27.462 528 306 17 77 549 42 547 2.98e-38 148
MS.gene070120.t1 AT1G30520 27.462 528 306 17 77 549 42 547 2.98e-38 148
MS.gene070120.t1 AT1G65880 26.376 527 332 16 66 549 38 551 5.00e-38 148
MS.gene070120.t1 AT5G16340 27.400 500 320 14 80 549 53 539 7.77e-37 144
MS.gene070120.t1 AT1G66120 27.672 524 321 17 66 549 38 543 8.22e-36 141
MS.gene070120.t1 AT3G16910 29.716 387 243 12 181 549 181 556 3.61e-35 139
MS.gene070120.t1 AT1G75960 30.623 369 227 13 196 548 183 538 1.62e-34 137
MS.gene070120.t1 AT1G20560 28.036 560 339 21 29 549 6 540 4.23e-32 130
MS.gene070120.t1 AT5G36880 25.328 533 344 13 67 555 147 669 3.85e-31 129
MS.gene070120.t1 AT5G36880 25.328 533 344 13 67 555 147 669 3.85e-31 129
MS.gene070120.t1 AT5G36880 25.328 533 344 13 67 555 147 669 3.85e-31 129
MS.gene070120.t1 AT5G36880 25.328 533 344 13 67 555 147 669 3.85e-31 129
MS.gene070120.t1 AT5G36880 25.328 533 344 13 67 555 197 719 4.39e-31 129
MS.gene070120.t1 AT3G16170 24.846 487 299 16 109 554 147 607 6.84e-31 127
MS.gene070120.t1 AT1G20560 30.623 369 223 12 198 549 110 462 8.70e-31 125
MS.gene070120.t1 AT1G76290 27.291 502 312 18 80 549 52 532 6.15e-30 124
MS.gene070120.t1 AT1G30520 26.622 447 262 15 77 482 42 463 1.98e-29 122
MS.gene070120.t1 AT1G30520 26.622 447 262 15 77 482 42 463 1.98e-29 122
MS.gene070120.t1 AT2G17650 27.913 369 238 13 196 549 240 595 1.01e-28 120
MS.gene070120.t1 AT5G27600 26.960 523 294 15 23 470 60 569 1.43e-25 111
MS.gene070120.t1 AT1G68270 22.770 527 318 13 66 548 38 519 6.39e-25 108
MS.gene070120.t1 AT5G36880 25.270 463 295 12 67 488 147 599 2.67e-24 107
MS.gene070120.t1 AT1G64400 24.758 517 294 19 21 456 21 523 9.61e-22 99.8
MS.gene070120.t1 AT1G49430 24.952 525 309 19 35 484 39 553 2.23e-21 98.6
MS.gene070120.t1 AT1G77590 24.385 488 286 15 68 482 107 584 8.39e-20 94.0
MS.gene070120.t1 AT3G05970 24.532 534 287 17 26 470 63 569 6.29e-19 91.3
MS.gene070120.t1 AT1G77590 26.801 347 184 13 198 482 100 438 3.45e-18 88.2
MS.gene070120.t1 AT3G23790 29.651 172 103 5 335 493 465 631 3.46e-12 69.7
MS.gene070120.t1 AT5G35930 23.303 545 303 22 69 542 59 559 9.32e-12 68.6
MS.gene070120.t1 AT5G35930 23.669 507 286 20 94 542 4 467 1.53e-11 67.8

Find 117 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTATTGATTCCTCTTC+TGG 0.119793 5.4:-18160741 MS.gene070120:CDS
GGCTTCTGGTCTTCATAAAA+TGG 0.204210 5.4:-18160673 MS.gene070120:CDS
ATGGGATAGTAAATTGAATT+TGG 0.204514 5.4:+18160615 None:intergenic
TATCAAATACAAGGGCTTTC+AGG 0.229499 5.4:-18159176 MS.gene070120:intron
TTGACATTGACGAGACTATT+AGG 0.230432 5.4:-18160080 MS.gene070120:CDS
TACTATCCCATTGTACTATT+AGG 0.254485 5.4:-18160603 MS.gene070120:CDS
CCCTTATTGATTCCTCTTCT+GGG 0.272214 5.4:-18160740 MS.gene070120:CDS
TTGCCGATGTTCCATTTATA+TGG 0.273927 5.4:-18160165 MS.gene070120:CDS
ACCTGTATAAAATCAACATT+AGG 0.277352 5.4:+18159886 None:intergenic
AGAGCGATAAACCATATAAA+TGG 0.308944 5.4:+18160154 None:intergenic
GCGAAGGCAAAGGGTGTAAA+TGG 0.324668 5.4:-18159994 MS.gene070120:CDS
TGCTACGGGATTATTGTCAT+TGG 0.327188 5.4:-18160130 MS.gene070120:CDS
TTAGTCAAATCAATGGCTTC+TGG 0.343881 5.4:-18160687 MS.gene070120:CDS
GAAATGCCTTAGAGTTTAGA+AGG 0.348615 5.4:+18158599 None:intergenic
ACAGCAACATCCACTATTTC+AGG 0.352555 5.4:+18159036 None:intergenic
AGCAGTTTCTGAGTTGCTTT+CGG 0.356180 5.4:+18158624 None:intergenic
ATAGTGGATGTTGCTGTTAC+AGG 0.372325 5.4:-18159030 MS.gene070120:CDS
ATGACTGAGTCGGGTGCATT+AGG 0.374967 5.4:-18159735 MS.gene070120:CDS
CTCCCCAGCTTCTTCATCCT+TGG 0.375919 5.4:+18158912 None:intergenic
ACTTTGCGAACCTTCTTGAA+TGG 0.377094 5.4:+18158692 None:intergenic
ACGAAATTGATAACATCCTT+TGG 0.383810 5.4:+18158844 None:intergenic
AACGATACAACGTCGAGAAA+AGG 0.386540 5.4:+18160795 None:intergenic
TGCTTGCCTTCTAAACTCTA+AGG 0.391570 5.4:-18158605 None:intergenic
GTGTCTTCTTGTTTAATAAC+CGG 0.393788 5.4:+18160339 None:intergenic
GGAGATTCCAGTGGCATTTG+TGG 0.394931 5.4:-18158893 MS.gene070120:CDS
TCCCAGAAGAGGAATCAATA+AGG 0.395051 5.4:+18160739 None:intergenic
TGACATTGACGAGACTATTA+GGG 0.397999 5.4:-18160079 MS.gene070120:CDS
GCTACGGGATTATTGTCATT+GGG 0.419178 5.4:-18160129 MS.gene070120:CDS
CAGGCAGAAGTGGTGAGCTT+TGG 0.427653 5.4:-18159602 MS.gene070120:CDS
AATCAACATTAGGGAAAGCT+TGG 0.430246 5.4:+18159896 None:intergenic
AAACAAAGTTACCAGAAATC+AGG 0.431755 5.4:+18160373 None:intergenic
GTTAAGAAATTAGAACCATT+AGG 0.435854 5.4:-18160465 MS.gene070120:CDS
TAACAACTTTGAGTAAGAAA+TGG 0.436153 5.4:+18160713 None:intergenic
TGGTTTATCGCTCTTTGCTA+CGG 0.436373 5.4:-18160145 MS.gene070120:CDS
TGGAGGCAAAAGTGGTAGAT+TGG 0.438591 5.4:-18159647 MS.gene070120:CDS
CCTGTATAAAATCAACATTA+GGG 0.441453 5.4:+18159887 None:intergenic
AAAGATGGTTGGCTACATAC+TGG 0.442873 5.4:-18159259 MS.gene070120:CDS
GCTGTCAATGCCGACAATAT+CGG 0.443469 5.4:+18160015 None:intergenic
GAAGCCATTGATTTGACTAA+AGG 0.445775 5.4:+18160690 None:intergenic
TGTTGTTCCACCGATATTGT+CGG 0.453280 5.4:-18160025 MS.gene070120:CDS
GTTACCTTTAGTCAAATCAA+TGG 0.458910 5.4:-18160694 MS.gene070120:CDS
GCATTTGTGGTCAAGAAAGT+TGG 0.466162 5.4:-18158880 MS.gene070120:CDS
ACGATACAACGTCGAGAAAA+GGG 0.466770 5.4:+18160796 None:intergenic
TTTCGCAACTATTCTTCACT+AGG 0.467126 5.4:-18159687 MS.gene070120:CDS
TTCTGTGCCAGAAAATGAAA+AGG 0.468038 5.4:-18160430 MS.gene070120:CDS
AAGATTCCGAGGTCTGTGAC+TGG 0.468807 5.4:-18158656 MS.gene070120:CDS
GCTTCTGGTCTTCATAAAAT+GGG 0.474868 5.4:-18160672 MS.gene070120:CDS
GGTTTATCGCTCTTTGCTAC+GGG 0.477009 5.4:-18160144 MS.gene070120:CDS
ATAACACCTAATAGTACAAT+GGG 0.478275 5.4:+18160597 None:intergenic
TATCAATTTCGTTGCTAAGC+AGG 0.483750 5.4:-18158833 MS.gene070120:intron
TTCTTGACCACAAATGCCAC+TGG 0.484108 5.4:+18158886 None:intergenic
TCATAGAAATCTTATTGCTA+TGG 0.487900 5.4:-18160259 MS.gene070120:CDS
GGGTCGGTCGGAAGGTTCCT+AGG 0.489518 5.4:+18160816 None:intergenic
CTTAGGATACTTGAATAATG+AGG 0.494593 5.4:-18159305 MS.gene070120:intron
ATTTCATCAACACTACTAAG+AGG 0.496577 5.4:+18160543 None:intergenic
CACAGACCTCGGAATCTTGT+CGG 0.496944 5.4:+18158661 None:intergenic
GAAAGAATTTATCAAATACA+AGG 0.497893 5.4:-18159185 MS.gene070120:CDS
TGTTGATGAAATTCGTAAGC+AGG 0.500410 5.4:-18160532 MS.gene070120:CDS
ATACCATCACTCACCCTCCT+TGG 0.503393 5.4:-18160878 MS.gene070120:CDS
CCCAGAAGAGGAATCAATAA+GGG 0.505561 5.4:+18160740 None:intergenic
GAAATAATAGGAATGCCTAA+TGG 0.507899 5.4:+18160450 None:intergenic
ATCAACATTAGGGAAAGCTT+GGG 0.510145 5.4:+18159897 None:intergenic
TAAGTTGCAGAGTTTGAGAC+AGG 0.511530 5.4:-18159968 MS.gene070120:CDS
AGCACAGCTTCTAAATCAGC+AGG 0.515337 5.4:+18159069 None:intergenic
GTTTATGGAATGACTGAGTC+GGG 0.516171 5.4:-18159744 MS.gene070120:CDS
GGTTTATGGAATGACTGAGT+CGG 0.521041 5.4:-18159745 MS.gene070120:CDS
ACAATAGTATACACTCTCCT+AGG 0.521378 5.4:-18160833 MS.gene070120:CDS
AGTGGTGAGCTTTGGTTGAG+AGG 0.521413 5.4:-18159594 MS.gene070120:CDS
AGTGCCAAGGATGAAGAAGC+TGG 0.523191 5.4:-18158916 MS.gene070120:CDS
CAAAGCTCACCACTTCTGCC+TGG 0.525545 5.4:+18159603 None:intergenic
GTGTATACTATTGTAAATAC+CGG 0.526191 5.4:+18160842 None:intergenic
ATGTCGACAATTGATAAAGA+TGG 0.526979 5.4:-18159274 MS.gene070120:CDS
TCCAAACATGGAGGCAAAAG+TGG 0.536470 5.4:-18159655 MS.gene070120:CDS
TTCGCAACTATTCTTCACTA+GGG 0.541708 5.4:-18159686 MS.gene070120:CDS
GCAGGTTGCGCCATTCAAGA+AGG 0.552672 5.4:-18158702 MS.gene070120:intron
GTGCCAAGGATGAAGAAGCT+GGG 0.553492 5.4:-18158915 MS.gene070120:CDS
CGAGAAAAGGGTCGGTCGGA+AGG 0.556286 5.4:+18160808 None:intergenic
CATAACACCTAATAGTACAA+TGG 0.557647 5.4:+18160596 None:intergenic
TGGAAGTGTACTTTCTCCAA+AGG 0.559244 5.4:-18158860 MS.gene070120:CDS
AAAGTGGTAGATTGGAAAAG+TGG 0.560810 5.4:-18159639 MS.gene070120:CDS
GCATTGACAGCGAAGGCAAA+GGG 0.561700 5.4:-18160003 MS.gene070120:CDS
TAGTGGATGTTGCTGTTACA+GGG 0.564429 5.4:-18159029 MS.gene070120:intron
ACACTACTAAGAGGATTCAA+TGG 0.565007 5.4:+18160552 None:intergenic
GGCATTGACAGCGAAGGCAA+AGG 0.570107 5.4:-18160004 MS.gene070120:CDS
ATTTGATAAATTCTTTCAAG+CGG 0.572308 5.4:+18159191 None:intergenic
AGAGGTCCTTCAATTATGAA+AGG 0.572900 5.4:-18159576 MS.gene070120:intron
CGACAATTGATAAAGATGGT+TGG 0.573781 5.4:-18159270 MS.gene070120:CDS
AGATTCCGAGGTCTGTGACT+GGG 0.575854 5.4:-18158655 MS.gene070120:CDS
ATCTTCCCAGTCACAGACCT+CGG 0.576821 5.4:+18158650 None:intergenic
AAAGAATTTATCAAATACAA+GGG 0.580755 5.4:-18159184 MS.gene070120:CDS
CATAAAATGGGAGTTTCACA+AGG 0.583635 5.4:-18160660 MS.gene070120:CDS
CTATTAGGTGTTATGTACCT+AGG 0.587403 5.4:-18160588 MS.gene070120:CDS
AAACCATATAAATGGAACAT+CGG 0.588130 5.4:+18160162 None:intergenic
TTGCAGAGTTTGAGACAGGT+CGG 0.589428 5.4:-18159964 MS.gene070120:CDS
GGTACTACTGGTGTGAGCAA+AGG 0.591807 5.4:-18160294 MS.gene070120:CDS
AGAAATGGAAGTCCCAGAAG+AGG 0.600253 5.4:+18160728 None:intergenic
AGGGCTAAAACCCCATCATG+AGG 0.601023 5.4:+18160759 None:intergenic
AGTTTCTGAGTTGCTTTCGG+AGG 0.613327 5.4:+18158627 None:intergenic
AGGGTTGTTAGCTCCAAACA+TGG 0.614345 5.4:-18159667 MS.gene070120:CDS
ATTCAAGAAGGTTCGCAAAG+TGG 0.619277 5.4:-18158690 MS.gene070120:CDS
TGCCAAGGATGAAGAAGCTG+GGG 0.626607 5.4:-18158914 MS.gene070120:CDS
AGCTCACCACTTCTGCCTGG+TGG 0.628152 5.4:+18159606 None:intergenic
TCGGGTGCATTAGGAGCATG+CGG 0.630001 5.4:-18159726 MS.gene070120:CDS
GTCAATGCCGACAATATCGG+TGG 0.636107 5.4:+18160018 None:intergenic
GGGCTAAAACCCCATCATGA+GGG 0.653343 5.4:+18160760 None:intergenic
CGTAAGCAGGTTAATGACTG+TGG 0.656452 5.4:-18160519 MS.gene070120:CDS
ACGTCGAGAAAAGGGTCGGT+CGG 0.657613 5.4:+18160804 None:intergenic
ATTGTCGGCATTGACAGCGA+AGG 0.665271 5.4:-18160010 MS.gene070120:CDS
ATACCAAGGAGGGTGAGTGA+TGG 0.666939 5.4:+18160875 None:intergenic
GTTCTACAATCGTTGTAATG+CGG 0.673134 5.4:-18160107 MS.gene070120:CDS
ACAGCTTCTAAATCAGCAGG+AGG 0.685957 5.4:+18159072 None:intergenic
TACAACGTCGAGAAAAGGGT+CGG 0.686772 5.4:+18160800 None:intergenic
ATGGTGTAACAACAGCACCT+AGG 0.700872 5.4:+18160571 None:intergenic
TTGGAAGTAAAAGCAAAACA+AGG 0.703794 5.4:+18160634 None:intergenic
TCTTCACCGACAAGATTCCG+AGG 0.707806 5.4:-18158667 MS.gene070120:CDS
AGAAGCTGGGGAGATTCCAG+TGG 0.713242 5.4:-18158902 MS.gene070120:CDS
GTTGTTAGCTCCAAACATGG+AGG 0.741487 5.4:-18159664 MS.gene070120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAATTTATCAAATACAA+GGG - chr5.4:18160335-18160354 MS.gene070120:CDS 15.0%
!! GTTTGATTAATAATTTCAAT+TGG + chr5.4:18160180-18160199 None:intergenic 15.0%
!! TTATATTTAAATGTCTAAAG+AGG + chr5.4:18160553-18160572 None:intergenic 15.0%
!!! ATGTAAAATTGTTTCTTTTT+AGG - chr5.4:18159753-18159772 MS.gene070120:intron 15.0%
!! AAACAACATTATTTGAATGA+TGG + chr5.4:18159707-18159726 None:intergenic 20.0%
!! ATTTGATAAATTCTTTCAAG+CGG + chr5.4:18160331-18160350 None:intergenic 20.0%
!! GAAAGAATTTATCAAATACA+AGG - chr5.4:18160334-18160353 MS.gene070120:CDS 20.0%
!! TAAATACTAAAAGGATAACA+TGG - chr5.4:18160005-18160024 MS.gene070120:CDS 20.0%
!!! AACTTTTTCCTTAATTTCTT+AGG - chr5.4:18160197-18160216 MS.gene070120:CDS 20.0%
!!! AATTTTAACCCTTTTTTTCT+TGG - chr5.4:18160795-18160814 MS.gene070120:CDS 20.0%
!!! ATTGTTTATTTTGATCAAGA+TGG - chr5.4:18160287-18160306 MS.gene070120:CDS 20.0%
!!! ATTGTTTCTTTTTAGGTTTA+TGG - chr5.4:18159760-18159779 MS.gene070120:intron 20.0%
!!! TCTAATTTCTTAACATTTTC+AGG + chr5.4:18159048-18159067 None:intergenic 20.0%
!!! TGTTTTCCTAGATTTTTTTA+AGG - chr5.4:18159722-18159741 MS.gene070120:CDS 20.0%
!!! TTTTTACCTTTCATAATTGA+AGG + chr5.4:18159952-18159971 None:intergenic 20.0%
! AAACCATATAAATGGAACAT+CGG + chr5.4:18159360-18159379 None:intergenic 25.0%
! ACCTGTATAAAATCAACATT+AGG + chr5.4:18159636-18159655 None:intergenic 25.0%
! AGGATAACATGGTAATATAA+AGG - chr5.4:18160016-18160035 MS.gene070120:CDS 25.0%
! ATAACACCTAATAGTACAAT+GGG + chr5.4:18158925-18158944 None:intergenic 25.0%
! ATGGGATAGTAAATTGAATT+TGG + chr5.4:18158907-18158926 None:intergenic 25.0%
! CCTGTATAAAATCAACATTA+GGG + chr5.4:18159635-18159654 None:intergenic 25.0%
! GTGTATACTATTGTAAATAC+CGG + chr5.4:18158680-18158699 None:intergenic 25.0%
! GTTAAGAAATTAGAACCATT+AGG - chr5.4:18159054-18159073 MS.gene070120:CDS 25.0%
! TAACAACTTTGAGTAAGAAA+TGG + chr5.4:18158809-18158828 None:intergenic 25.0%
! TCAAGTATCCTAAGAAATTA+AGG + chr5.4:18160208-18160227 None:intergenic 25.0%
! TCATAGAAATCTTATTGCTA+TGG - chr5.4:18159260-18159279 MS.gene070120:CDS 25.0%
!! TTTATAGCTTTTGTGCTTAA+AGG + chr5.4:18159594-18159613 None:intergenic 25.0%
!!! TAAAGGTTTTATCTATTGTG+TGG - chr5.4:18160033-18160052 MS.gene070120:CDS 25.0%
!!! TGTTTTTTTAACCTGATTTC+TGG - chr5.4:18159135-18159154 MS.gene070120:intron 25.0%
AAACAAAGTTACCAGAAATC+AGG + chr5.4:18159149-18159168 None:intergenic 30.0%
AGAGCGATAAACCATATAAA+TGG + chr5.4:18159368-18159387 None:intergenic 30.0%
ATGTCGACAATTGATAAAGA+TGG - chr5.4:18160245-18160264 MS.gene070120:CDS 30.0%
ATTTCATCAACACTACTAAG+AGG + chr5.4:18158979-18158998 None:intergenic 30.0%
CATAACACCTAATAGTACAA+TGG + chr5.4:18158926-18158945 None:intergenic 30.0%
CTTAGGATACTTGAATAATG+AGG - chr5.4:18160214-18160233 MS.gene070120:CDS 30.0%
GAAATAATAGGAATGCCTAA+TGG + chr5.4:18159072-18159091 None:intergenic 30.0%
GCACATCCTTAAAAAAATCT+AGG + chr5.4:18159731-18159750 None:intergenic 30.0%
GCTTGAATCAATGTTAGTAT+TGG - chr5.4:18160761-18160780 MS.gene070120:CDS 30.0%
GGAAAATGATGAAACACTTA+AGG + chr5.4:18160576-18160595 None:intergenic 30.0%
GTTACCTTTAGTCAAATCAA+TGG - chr5.4:18158825-18158844 MS.gene070120:intron 30.0%
TACTATCCCATTGTACTATT+AGG - chr5.4:18158916-18158935 MS.gene070120:CDS 30.0%
TTGGAAGTAAAAGCAAAACA+AGG + chr5.4:18158888-18158907 None:intergenic 30.0%
! AACAGCTAGATACACATTTT+TGG + chr5.4:18159334-18159353 None:intergenic 30.0%
! AATTTTGCATCCTGAAATAG+TGG - chr5.4:18160473-18160492 MS.gene070120:CDS 30.0%
! ACGAAATTGATAACATCCTT+TGG + chr5.4:18160678-18160697 None:intergenic 30.0%
! ATCGAATCCTTTTCATTTTC+TGG + chr5.4:18159099-18159118 None:intergenic 30.0%
! GTGTCTTCTTGTTTAATAAC+CGG + chr5.4:18159183-18159202 None:intergenic 30.0%
!! TTTTCTGGCACAGAAATAAT+AGG + chr5.4:18159084-18159103 None:intergenic 30.0%
!!! CCCTAATGTTGATTTTATAC+AGG - chr5.4:18159632-18159651 MS.gene070120:CDS 30.0%
!!! CTATTGTAAATACCGGTTTT+TGG + chr5.4:18158673-18158692 None:intergenic 30.0%
AAAGTGGTAGATTGGAAAAG+TGG - chr5.4:18159880-18159899 MS.gene070120:intron 35.0%
AATCAACATTAGGGAAAGCT+TGG + chr5.4:18159626-18159645 None:intergenic 35.0%
AATTGGAATAGCAACAAGAC+AGG + chr5.4:18160163-18160182 None:intergenic 35.0%
ACAATAGTATACACTCTCCT+AGG - chr5.4:18158686-18158705 MS.gene070120:CDS 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr5.4:18158970-18158989 None:intergenic 35.0%
AGAGGTCCTTCAATTATGAA+AGG - chr5.4:18159943-18159962 MS.gene070120:CDS 35.0%
ATCAACATTAGGGAAAGCTT+GGG + chr5.4:18159625-18159644 None:intergenic 35.0%
CATAAAATGGGAGTTTCACA+AGG - chr5.4:18158859-18158878 MS.gene070120:CDS 35.0%
CGACAATTGATAAAGATGGT+TGG - chr5.4:18160249-18160268 MS.gene070120:CDS 35.0%
GTTCTACAATCGTTGTAATG+CGG - chr5.4:18159412-18159431 MS.gene070120:intron 35.0%
TATCAAATACAAGGGCTTTC+AGG - chr5.4:18160343-18160362 MS.gene070120:CDS 35.0%
TATCAATTTCGTTGCTAAGC+AGG - chr5.4:18160686-18160705 MS.gene070120:CDS 35.0%
TGACATTGACGAGACTATTA+GGG - chr5.4:18159440-18159459 MS.gene070120:intron 35.0%
TTAGTCAAATCAATGGCTTC+TGG - chr5.4:18158832-18158851 MS.gene070120:intron 35.0%
TTCGCAACTATTCTTCACTA+GGG - chr5.4:18159833-18159852 MS.gene070120:intron 35.0%
TTCTGTGCCAGAAAATGAAA+AGG - chr5.4:18159089-18159108 MS.gene070120:intron 35.0%
TTGACATTGACGAGACTATT+AGG - chr5.4:18159439-18159458 MS.gene070120:intron 35.0%
TTGCCGATGTTCCATTTATA+TGG - chr5.4:18159354-18159373 MS.gene070120:intron 35.0%
TTTCGCAACTATTCTTCACT+AGG - chr5.4:18159832-18159851 MS.gene070120:intron 35.0%
! CTATTAGGTGTTATGTACCT+AGG - chr5.4:18158931-18158950 MS.gene070120:intron 35.0%
! GCTGCTATATTGTTTTCTTC+TGG - chr5.4:18159204-18159223 MS.gene070120:CDS 35.0%
! GCTTCTGGTCTTCATAAAAT+GGG - chr5.4:18158847-18158866 MS.gene070120:CDS 35.0%
! TGTTGATGAAATTCGTAAGC+AGG - chr5.4:18158987-18159006 MS.gene070120:intron 35.0%
! TTGTTTTCTTCTGGTACTAC+TGG - chr5.4:18159213-18159232 MS.gene070120:CDS 35.0%
! TTTTCCATATATCAGTGCCA+AGG - chr5.4:18160590-18160609 MS.gene070120:CDS 35.0%
!! GAAGCCATTGATTTGACTAA+AGG + chr5.4:18158832-18158851 None:intergenic 35.0%
!! TGTTTAATAACCGGCTTTTG+AGG + chr5.4:18159174-18159193 None:intergenic 35.0%
!! TTTTTGGTGAATACCAAGGA+GGG + chr5.4:18158657-18158676 None:intergenic 35.0%
!!! ATTTTTTCTCACCCTCATGA+TGG - chr5.4:18158748-18158767 MS.gene070120:intron 35.0%
!!! TTAACCCTTTTTTTCTTGGC+AGG - chr5.4:18160799-18160818 MS.gene070120:CDS 35.0%
!!! TTTTTTCTCACCCTCATGAT+GGG - chr5.4:18158749-18158768 MS.gene070120:intron 35.0%
AACGATACAACGTCGAGAAA+AGG + chr5.4:18158727-18158746 None:intergenic 40.0%
ACAGCAACATCCACTATTTC+AGG + chr5.4:18160486-18160505 None:intergenic 40.0%
ACGATACAACGTCGAGAAAA+GGG + chr5.4:18158726-18158745 None:intergenic 40.0%
ACTTTGCGAACCTTCTTGAA+TGG + chr5.4:18160830-18160849 None:intergenic 40.0%
ATTCAAGAAGGTTCGCAAAG+TGG - chr5.4:18160829-18160848 MS.gene070120:CDS 40.0%
CCCAGAAGAGGAATCAATAA+GGG + chr5.4:18158782-18158801 None:intergenic 40.0%
CCTTGGTATTCACCAAAAAC+CGG - chr5.4:18158658-18158677 MS.gene070120:CDS 40.0%
GCAACCTGCCAAGAAAAAAA+GGG + chr5.4:18160806-18160825 None:intergenic 40.0%
GCATTTGTGGTCAAGAAAGT+TGG - chr5.4:18160639-18160658 MS.gene070120:CDS 40.0%
GCTACGGGATTATTGTCATT+GGG - chr5.4:18159390-18159409 MS.gene070120:intron 40.0%
GGTTTATGGAATGACTGAGT+CGG - chr5.4:18159774-18159793 MS.gene070120:intron 40.0%
GTTTATGGAATGACTGAGTC+GGG - chr5.4:18159775-18159794 MS.gene070120:intron 40.0%
TCCCAGAAGAGGAATCAATA+AGG + chr5.4:18158783-18158802 None:intergenic 40.0%
TGCTACGGGATTATTGTCAT+TGG - chr5.4:18159389-18159408 MS.gene070120:intron 40.0%
TGGTTTATCGCTCTTTGCTA+CGG - chr5.4:18159374-18159393 MS.gene070120:intron 40.0%
TGTTGTTCCACCGATATTGT+CGG - chr5.4:18159494-18159513 MS.gene070120:intron 40.0%
! AAAGATGGTTGGCTACATAC+TGG - chr5.4:18160260-18160279 MS.gene070120:CDS 40.0%
! AGCAGTTTCTGAGTTGCTTT+CGG + chr5.4:18160898-18160917 None:intergenic 40.0%
! CTATGGTTGAGCTTTTTGTG+AGG - chr5.4:18159277-18159296 MS.gene070120:CDS 40.0%
! GGCTTCTGGTCTTCATAAAA+TGG - chr5.4:18158846-18158865 MS.gene070120:CDS 40.0%
! TAAGTTGCAGAGTTTGAGAC+AGG - chr5.4:18159551-18159570 MS.gene070120:intron 40.0%
! TAGTGGATGTTGCTGTTACA+GGG - chr5.4:18160490-18160509 MS.gene070120:CDS 40.0%
! TGGAAGTGTACTTTCTCCAA+AGG - chr5.4:18160659-18160678 MS.gene070120:CDS 40.0%
! TTTTTCTCACCCTCATGATG+GGG - chr5.4:18158750-18158769 MS.gene070120:intron 40.0%
!! ATAGTGGATGTTGCTGTTAC+AGG - chr5.4:18160489-18160508 MS.gene070120:CDS 40.0%
!! CCCTTATTGATTCCTCTTCT+GGG - chr5.4:18158779-18158798 MS.gene070120:intron 40.0%
!! TCATCCTTGGCACTGATATA+TGG + chr5.4:18160597-18160616 None:intergenic 40.0%
!!! CTTTGTTTTGCCTCAAAAGC+CGG - chr5.4:18159161-18159180 MS.gene070120:intron 40.0%
!!! GTTTTTGGTGAATACCAAGG+AGG + chr5.4:18158658-18158677 None:intergenic 40.0%
ACAGCTTCTAAATCAGCAGG+AGG + chr5.4:18160450-18160469 None:intergenic 45.0%
ACAGGCAGAAAAGGTTCAAG+TGG + chr5.4:18160145-18160164 None:intergenic 45.0%
AGAAATGGAAGTCCCAGAAG+AGG + chr5.4:18158794-18158813 None:intergenic 45.0%
AGCAACAAGACAGGCAGAAA+AGG + chr5.4:18160154-18160173 None:intergenic 45.0%
AGCACAGCTTCTAAATCAGC+AGG + chr5.4:18160453-18160472 None:intergenic 45.0%
AGGGTTGTTAGCTCCAAACA+TGG - chr5.4:18159852-18159871 MS.gene070120:intron 45.0%
ATGGTGTAACAACAGCACCT+AGG + chr5.4:18158951-18158970 None:intergenic 45.0%
CGCAACCTGCCAAGAAAAAA+AGG + chr5.4:18160807-18160826 None:intergenic 45.0%
CGTAAGCAGGTTAATGACTG+TGG - chr5.4:18159000-18159019 MS.gene070120:intron 45.0%
GCTGTCAATGCCGACAATAT+CGG + chr5.4:18159507-18159526 None:intergenic 45.0%
GGTTTATCGCTCTTTGCTAC+GGG - chr5.4:18159375-18159394 MS.gene070120:intron 45.0%
GTTGTTAGCTCCAAACATGG+AGG - chr5.4:18159855-18159874 MS.gene070120:intron 45.0%
TACAACGTCGAGAAAAGGGT+CGG + chr5.4:18158722-18158741 None:intergenic 45.0%
TCCAAACATGGAGGCAAAAG+TGG - chr5.4:18159864-18159883 MS.gene070120:intron 45.0%
TGGAGGCAAAAGTGGTAGAT+TGG - chr5.4:18159872-18159891 MS.gene070120:intron 45.0%
TTCTTGACCACAAATGCCAC+TGG + chr5.4:18160636-18160655 None:intergenic 45.0%
! AGTTTCTGAGTTGCTTTCGG+AGG + chr5.4:18160895-18160914 None:intergenic 45.0%
! GGGTGAGTGATGGTATTTTG+TGG + chr5.4:18158637-18158656 None:intergenic 45.0%
! TTGCAGAGTTTGAGACAGGT+CGG - chr5.4:18159555-18159574 MS.gene070120:intron 45.0%
!! ACCACTTTTGCCTCCATGTT+TGG + chr5.4:18159868-18159887 None:intergenic 45.0%
!! GCCCTTATTGATTCCTCTTC+TGG - chr5.4:18158778-18158797 MS.gene070120:intron 45.0%
!!! CCGGTTTTTGGTGAATACCA+AGG + chr5.4:18158661-18158680 None:intergenic 45.0%
!! TAATAAATATAAATACTAAA+AGG - chr5.4:18159996-18160015 MS.gene070120:CDS 5.0%
AAGATTCCGAGGTCTGTGAC+TGG - chr5.4:18160863-18160882 MS.gene070120:CDS 50.0%
AGATTCCGAGGTCTGTGACT+GGG - chr5.4:18160864-18160883 MS.gene070120:CDS 50.0%
AGGGCTAAAACCCCATCATG+AGG + chr5.4:18158763-18158782 None:intergenic 50.0%
AGTGCCAAGGATGAAGAAGC+TGG - chr5.4:18160603-18160622 MS.gene070120:CDS 50.0%
ATACCAAGGAGGGTGAGTGA+TGG + chr5.4:18158647-18158666 None:intergenic 50.0%
ATACCATCACTCACCCTCCT+TGG - chr5.4:18158641-18158660 MS.gene070120:CDS 50.0%
ATCTTCCCAGTCACAGACCT+CGG + chr5.4:18160872-18160891 None:intergenic 50.0%
ATGACTGAGTCGGGTGCATT+AGG - chr5.4:18159784-18159803 MS.gene070120:intron 50.0%
CACAGACCTCGGAATCTTGT+CGG + chr5.4:18160861-18160880 None:intergenic 50.0%
GGAGATTCCAGTGGCATTTG+TGG - chr5.4:18160626-18160645 MS.gene070120:CDS 50.0%
GGGCTAAAACCCCATCATGA+GGG + chr5.4:18158762-18158781 None:intergenic 50.0%
GGTACTACTGGTGTGAGCAA+AGG - chr5.4:18159225-18159244 MS.gene070120:CDS 50.0%
GTCAATGCCGACAATATCGG+TGG + chr5.4:18159504-18159523 None:intergenic 50.0%
GTGCCAAGGATGAAGAAGCT+GGG - chr5.4:18160604-18160623 MS.gene070120:CDS 50.0%
TCTTCACCGACAAGATTCCG+AGG - chr5.4:18160852-18160871 MS.gene070120:CDS 50.0%
TGCCAAGGATGAAGAAGCTG+GGG - chr5.4:18160605-18160624 MS.gene070120:CDS 50.0%
! AGTGGTGAGCTTTGGTTGAG+AGG - chr5.4:18159925-18159944 MS.gene070120:CDS 50.0%
! GCATTGACAGCGAAGGCAAA+GGG - chr5.4:18159516-18159535 MS.gene070120:intron 50.0%
! GCGAAGGCAAAGGGTGTAAA+TGG - chr5.4:18159525-18159544 MS.gene070120:intron 50.0%
! TTTTTGCCACCAGGCAGAAG+TGG - chr5.4:18159907-18159926 MS.gene070120:CDS 50.0%
!! ATTGTCGGCATTGACAGCGA+AGG - chr5.4:18159509-18159528 MS.gene070120:intron 50.0%
!!! AGTGGTGCTTTTTTGCCACC+AGG - chr5.4:18159898-18159917 MS.gene070120:CDS 50.0%
ACGTCGAGAAAAGGGTCGGT+CGG + chr5.4:18158718-18158737 None:intergenic 55.0%
AGAAGCTGGGGAGATTCCAG+TGG - chr5.4:18160617-18160636 MS.gene070120:CDS 55.0%
CAAAGCTCACCACTTCTGCC+TGG + chr5.4:18159919-18159938 None:intergenic 55.0%
CAGGCAGAAGTGGTGAGCTT+TGG - chr5.4:18159917-18159936 MS.gene070120:CDS 55.0%
CTCCCCAGCTTCTTCATCCT+TGG + chr5.4:18160610-18160629 None:intergenic 55.0%
GCAGGTTGCGCCATTCAAGA+AGG - chr5.4:18160817-18160836 MS.gene070120:CDS 55.0%
! TCGGGTGCATTAGGAGCATG+CGG - chr5.4:18159793-18159812 MS.gene070120:intron 55.0%
!! GGCATTGACAGCGAAGGCAA+AGG - chr5.4:18159515-18159534 MS.gene070120:intron 55.0%
AGCTCACCACTTCTGCCTGG+TGG + chr5.4:18159916-18159935 None:intergenic 60.0%
CGAGAAAAGGGTCGGTCGGA+AGG + chr5.4:18158714-18158733 None:intergenic 60.0%
GGGTCGGTCGGAAGGTTCCT+AGG + chr5.4:18158706-18158725 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 18158607 18160934 18158607 ID=MS.gene070120
chr5.4 mRNA 18158607 18160934 18158607 ID=MS.gene070120.t1;Parent=MS.gene070120
chr5.4 exon 18159888 18160934 18159888 ID=MS.gene070120.t1.exon1;Parent=MS.gene070120.t1
chr5.4 CDS 18159888 18160934 18159888 ID=cds.MS.gene070120.t1;Parent=MS.gene070120.t1
chr5.4 exon 18159577 18159766 18159577 ID=MS.gene070120.t1.exon2;Parent=MS.gene070120.t1
chr5.4 CDS 18159577 18159766 18159577 ID=cds.MS.gene070120.t1;Parent=MS.gene070120.t1
chr5.4 exon 18159177 18159322 18159177 ID=MS.gene070120.t1.exon3;Parent=MS.gene070120.t1
chr5.4 CDS 18159177 18159322 18159177 ID=cds.MS.gene070120.t1;Parent=MS.gene070120.t1
chr5.4 exon 18159030 18159097 18159030 ID=MS.gene070120.t1.exon4;Parent=MS.gene070120.t1
chr5.4 CDS 18159030 18159097 18159030 ID=cds.MS.gene070120.t1;Parent=MS.gene070120.t1
chr5.4 exon 18158834 18158936 18158834 ID=MS.gene070120.t1.exon5;Parent=MS.gene070120.t1
chr5.4 CDS 18158834 18158936 18158834 ID=cds.MS.gene070120.t1;Parent=MS.gene070120.t1
chr5.4 exon 18158607 18158720 18158607 ID=MS.gene070120.t1.exon6;Parent=MS.gene070120.t1
chr5.4 CDS 18158607 18158720 18158607 ID=cds.MS.gene070120.t1;Parent=MS.gene070120.t1
Gene Sequence

>MS.gene070120

ATGGCTGCAAATTTCAACTATGACATGCCACAAAATACCATCACTCACCCTCCTTGGTATTCACCAAAAACCGGTATTTACAATAGTATACACTCTCCTAGGAACCTTCCGACCGACCCTTTTCTCGACGTTGTATCGTTCATTTTTTCTCACCCTCATGATGGGGTTTTAGCCCTTATTGATTCCTCTTCTGGGACTTCCATTTCTTACTCAAAGTTGTTACCTTTAGTCAAATCAATGGCTTCTGGTCTTCATAAAATGGGAGTTTCACAAGGTGACCTTGTTTTGCTTTTACTTCCAAATTCAATTTACTATCCCATTGTACTATTAGGTGTTATGTACCTAGGTGCTGTTGTTACACCATTGAATCCTCTTAGTAGTGTTGATGAAATTCGTAAGCAGGTTAATGACTGTGGTGTGAGTTTTGCTTTTACTATACCTGAAAATGTTAAGAAATTAGAACCATTAGGCATTCCTATTATTTCTGTGCCAGAAAATGAAAAGGATTCGATGAATGATTGTTTCTCATGTTTTTTTAACCTGATTTCTGGTAACTTTGTTTTGCCTCAAAAGCCGGTTATTAAACAAGAAGACACTGCTGCTATATTGTTTTCTTCTGGTACTACTGGTGTGAGCAAAGGAGTTGTTTTAACTCATAGAAATCTTATTGCTATGGTTGAGCTTTTTGTGAGGTTTGAAGCTTCTCAATATGAATACTCTAGTTCCAAAAATGTGTATCTAGCTGTTTTGCCGATGTTCCATTTATATGGTTTATCGCTCTTTGCTACGGGATTATTGTCATTGGGTTCTACAATCGTTGTAATGCGGAAATTTGACATTGACGAGACTATTAGGGTGATCGATAAATATAATGTTACACATTTTCATGTTGTTCCACCGATATTGTCGGCATTGACAGCGAAGGCAAAGGGTGTAAATGGAAGTAAGTTGCAGAGTTTGAGACAGGTCGGTTCTAGTGCAGCGCCTTTAAGCACAAAAGCTATAAATGATTTTGCCCAAGCTTTCCCTAATGTTGATTTTATACAGGTAAAAATCTCAGAATAAAATAGTCTTATTTAAAGAATGCAAGTCGTGATCCATCATTCAAATAATGTTGTTTTCCTAGATTTTTTTAAGGATGTGCAGATGTAAAATTGTTTCTTTTTAGGTTTATGGAATGACTGAGTCGGGTGCATTAGGAGCATGCGGTTTCAATACAGAAAAATTTCGCAACTATTCTTCACTAGGGTTGTTAGCTCCAAACATGGAGGCAAAAGTGGTAGATTGGAAAAGTGGTGCTTTTTTGCCACCAGGCAGAAGTGGTGAGCTTTGGTTGAGAGGTCCTTCAATTATGAAAGGTAAAAATAATCTGCATTTTTTATGAATAGATAATAAATATAAATACTAAAAGGATAACATGGTAATATAAAGGTTTTATCTATTGTGTGGAATCTTAATCTACATTAGCATGAGATTGATTCAATGATTTCAACTGTTTTGTTGCTGTAACTTTTGTTTCTTTCTTATTTCTCAAGCCACTTGAACCTTTTCTGCCTGTCTTGTTGCTATTCCAATTGAAATTATTAATCAAACTTTTTCCTTAATTTCTTAGGATACTTGAATAATGAGGAAGCTACAATGTCGACAATTGATAAAGATGGTTGGCTACATACTGGAGATATTGTTTATTTTGATCAAGATGGATATTTATATTTGTCTGACCGCTTGAAAGAATTTATCAAATACAAGGGCTTTCAGGTATCAATTTATTTTACAGTTTATTTTTAATCAATTTGTATGCAATATGTTCTTATGTATATCACACATGCTGTTACAGATTCCTCCTGCTGATTTAGAAGCTGTGCTAATTTTGCATCCTGAAATAGTGGATGTTGCTGTTACAGGGTAAGTATATGAACACTTCATTTATGTCTTCTAAGATGCCTCTTTAGACATTTAAATATAACCTTAAGTGTTTCATCATTTTCCATATATCAGTGCCAAGGATGAAGAAGCTGGGGAGATTCCAGTGGCATTTGTGGTCAAGAAAGTTGGAAGTGTACTTTCTCCAAAGGATGTTATCAATTTCGTTGCTAAGCAGGTTCTTCTTTATAAGACAGACATGAGTTCTATGATATTTGAATTAATGTCACTGCTTGAATCAATGTTAGTATTGGAATGATTTTCAAATTTTAACCCTTTTTTTCTTGGCAGGTTGCGCCATTCAAGAAGGTTCGCAAAGTGGTCTTCACCGACAAGATTCCGAGGTCTGTGACTGGGAAGATCCTCCGAAAGCAACTCAGAAACTGCTTGCCTTCTAAACTCTAA
Protein sequence

>MS.gene070120.t1

MAANFNYDMPQNTITHPPWYSPKTGIYNSIHSPRNLPTDPFLDVVSFIFSHPHDGVLALIDSSSGTSISYSKLLPLVKSMASGLHKMGVSQGDLVLLLLPNSIYYPIVLLGVMYLGAVVTPLNPLSSVDEIRKQVNDCGVSFAFTIPENVKKLEPLGIPIISVPENEKDSMNDCFSCFFNLISGNFVLPQKPVIKQEDTAAILFSSGTTGVSKGVVLTHRNLIAMVELFVRFEASQYEYSSSKNVYLAVLPMFHLYGLSLFATGLLSLGSTIVVMRKFDIDETIRVIDKYNVTHFHVVPPILSALTAKAKGVNGSKLQSLRQVGSSAAPLSTKAINDFAQAFPNVDFIQVYGMTESGALGACGFNTEKFRNYSSLGLLAPNMEAKVVDWKSGAFLPPGRSGELWLRGPSIMKGYLNNEEATMSTIDKDGWLHTGDIVYFDQDGYLYLSDRLKEFIKYKGFQIPPADLEAVLILHPEIVDVAVTGAKDEEAGEIPVAFVVKKVGSVLSPKDVINFVAKQVAPFKKVRKVVFTDKIPRSVTGKILRKQLRNCLPSKL