AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067382

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067382.t1 MTR_5g027500 95.951 568 23 0 1 568 1 568 0.0 1102
MS.gene067382.t1 MTR_5g027480 81.690 568 91 2 1 568 1 555 0.0 935
MS.gene067382.t1 MTR_4g077900 80.408 490 82 2 81 568 1 478 0.0 797
MS.gene067382.t1 MTR_1g026310 43.423 555 295 6 23 568 16 560 1.57e-153 452
MS.gene067382.t1 MTR_8g039720 43.672 561 303 7 12 568 8 559 1.63e-151 447
MS.gene067382.t1 MTR_1g090737 45.668 554 265 11 21 560 27 558 2.69e-144 429
MS.gene067382.t1 MTR_4g057730 41.499 547 295 9 21 560 6 534 8.23e-136 406
MS.gene067382.t1 MTR_2g095510 39.344 549 308 8 26 568 10 539 1.28e-130 392
MS.gene067382.t1 MTR_3g088880 41.535 508 264 9 59 560 68 548 7.68e-129 389
MS.gene067382.t1 MTR_1g063120 39.318 557 307 10 19 560 4 544 1.23e-127 385
MS.gene067382.t1 MTR_3g088870 41.304 506 271 7 59 560 67 550 3.40e-127 384
MS.gene067382.t1 MTR_1g063110 38.612 562 319 8 19 568 4 551 6.72e-124 375
MS.gene067382.t1 MTR_1g073180 39.434 530 280 9 43 562 30 528 5.10e-113 347
MS.gene067382.t1 MTR_4g057730 41.079 482 259 9 21 495 6 469 8.24e-111 339
MS.gene067382.t1 MTR_2g105570 40.392 510 286 9 59 561 44 542 7.91e-110 339
MS.gene067382.t1 MTR_4g128337 37.109 512 292 10 59 560 49 540 1.65e-101 318
MS.gene067382.t1 MTR_1g073180 37.965 511 276 9 43 543 52 531 8.03e-101 315
MS.gene067382.t1 MTR_4g005750 39.062 512 284 10 59 561 43 535 3.71e-100 314
MS.gene067382.t1 MTR_4g057730 39.525 463 255 9 21 476 6 450 1.34e-99 310
MS.gene067382.t1 MTR_3g460770 34.111 557 343 10 17 568 4 541 1.68e-98 310
MS.gene067382.t1 MTR_5g007640 37.622 513 291 8 59 561 87 580 2.72e-98 310
MS.gene067382.t1 MTR_2g095510 37.174 460 264 8 26 479 10 450 4.64e-94 295
MS.gene067382.t1 MTR_1g026430 37.626 396 228 6 23 409 16 401 1.92e-78 253
MS.gene067382.t1 MTR_1g026470 37.626 396 228 6 23 409 16 401 5.77e-78 252
MS.gene067382.t1 MTR_3g035130 28.858 499 321 12 69 558 32 505 4.43e-50 181
MS.gene067382.t1 MTR_1569s0010 26.616 526 336 12 69 562 22 529 1.65e-43 163
MS.gene067382.t1 MTR_5g076240 27.533 523 334 17 67 562 38 542 1.68e-42 160
MS.gene067382.t1 MTR_3g035130 28.256 453 291 12 69 512 32 459 3.11e-38 147
MS.gene067382.t1 MTR_3g064420 26.071 537 332 15 65 561 36 547 2.71e-37 146
MS.gene067382.t1 MTR_2g044180 27.308 509 328 14 81 561 58 552 8.63e-37 144
MS.gene067382.t1 MTR_2g044180 29.581 382 242 10 197 561 90 461 1.00e-35 140
MS.gene067382.t1 MTR_8g074040 27.789 511 314 18 81 562 94 578 1.19e-35 141
MS.gene067382.t1 MTR_0803s0020 27.462 528 283 16 71 554 263 734 1.68e-35 142
MS.gene067382.t1 MTR_4g011790 27.595 395 253 10 191 561 207 592 2.29e-35 140
MS.gene067382.t1 MTR_4g013210 28.278 389 236 11 210 567 69 445 7.88e-35 137
MS.gene067382.t1 MTR_5g008800 29.365 504 313 16 81 562 52 534 1.35e-34 138
MS.gene067382.t1 MTR_4g121600 26.386 523 337 16 70 561 47 552 3.17e-34 137
MS.gene067382.t1 MTR_4g011790 27.506 389 249 10 191 555 207 586 5.88e-34 137
MS.gene067382.t1 MTR_4g011790 27.228 404 252 12 191 561 207 601 1.53e-33 135
MS.gene067382.t1 MTR_5g076220 29.076 368 231 10 211 561 188 542 1.20e-32 132
MS.gene067382.t1 MTR_0161s0050 28.649 370 234 10 209 561 188 544 1.57e-32 132
MS.gene067382.t1 MTR_8g074040 29.348 368 229 10 211 562 108 460 4.97e-32 129
MS.gene067382.t1 MTR_5g008820 28.291 509 308 18 81 561 52 531 6.93e-32 130
MS.gene067382.t1 MTR_8g074120 29.512 410 222 17 184 562 116 489 2.37e-30 125
MS.gene067382.t1 MTR_2g030240 26.296 540 318 16 72 561 31 540 5.01e-30 124
MS.gene067382.t1 MTR_7g084850 24.643 560 366 15 44 568 186 724 1.76e-29 124
MS.gene067382.t1 MTR_5g076200 26.316 532 332 16 65 561 36 542 6.93e-29 121
MS.gene067382.t1 MTR_4g013210 26.790 377 216 9 210 567 69 404 1.50e-28 118
MS.gene067382.t1 MTR_1g026350 42.537 134 77 0 21 154 20 153 1.98e-28 112
MS.gene067382.t1 MTR_4g013210 26.790 377 216 9 210 567 221 556 4.65e-28 118
MS.gene067382.t1 MTR_4g013210 28.212 358 214 11 210 536 221 566 7.45e-28 118
MS.gene067382.t1 MTR_5g023730 26.772 508 320 21 81 561 55 537 1.42e-27 117
MS.gene067382.t1 MTR_2g030240 26.302 384 232 9 215 561 77 446 1.97e-24 107
MS.gene067382.t1 MTR_3g087980 24.544 493 280 16 69 488 106 579 1.44e-23 105
MS.gene067382.t1 MTR_1g030290 24.900 498 272 19 69 488 108 581 6.85e-23 103
MS.gene067382.t1 MTR_1g104810 26.374 546 319 20 7 485 39 568 1.62e-22 102
MS.gene067382.t1 MTR_4g057755 55.556 72 32 0 424 495 46 117 5.91e-22 92.0
MS.gene067382.t1 MTR_1g104810 30.675 326 165 13 208 485 189 501 2.59e-21 98.6
MS.gene067382.t1 MTR_1054s0020 24.655 507 342 15 66 562 294 770 1.21e-20 96.7
MS.gene067382.t1 MTR_2g098490 26.768 396 234 16 211 557 647 1035 9.37e-19 91.3
MS.gene067382.t1 MTR_3g082660 26.705 352 179 14 202 486 272 611 4.42e-18 88.6
MS.gene067382.t1 MTR_8g083170 31.364 220 129 7 339 545 461 671 1.63e-17 86.7
MS.gene067382.t1 MTR_7g109830 23.295 528 319 16 38 495 36 547 3.99e-17 85.5
MS.gene067382.t1 MTR_4g013210 27.018 285 158 8 210 485 221 464 1.12e-16 83.6
MS.gene067382.t1 MTR_3g084450 35.088 171 88 7 328 481 233 397 1.63e-16 82.8
MS.gene067382.t1 MTR_3g084470 36.986 146 76 5 348 481 460 601 2.42e-16 82.8
MS.gene067382.t1 MTR_3g084450 35.088 171 88 7 328 481 441 605 3.36e-16 82.4
MS.gene067382.t1 MTR_3g088890 48.438 64 33 0 470 533 10 73 1.18e-13 67.4
MS.gene067382.t1 MTR_4g013210 27.126 247 148 9 210 436 221 455 2.37e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067382.t1 AT4G19010 59.239 552 213 4 18 568 22 562 0.0 654
MS.gene067382.t1 AT1G20510 44.669 544 278 8 28 566 17 542 5.74e-160 468
MS.gene067382.t1 AT1G20480 42.576 559 290 9 21 568 27 565 1.29e-149 442
MS.gene067382.t1 AT1G20500 42.936 545 290 8 28 568 23 550 1.17e-143 426
MS.gene067382.t1 AT4G05160 40.472 551 308 8 24 568 8 544 1.22e-136 408
MS.gene067382.t1 AT5G38120 40.993 544 301 8 28 568 24 550 2.83e-135 405
MS.gene067382.t1 AT1G20510 43.975 473 242 8 28 495 17 471 3.48e-133 397
MS.gene067382.t1 AT5G63380 39.279 555 301 9 21 560 17 550 1.19e-130 393
MS.gene067382.t1 AT1G20510 42.384 453 238 8 28 475 17 451 5.75e-120 363
MS.gene067382.t1 AT5G38120 39.961 508 285 8 28 532 24 514 6.22e-120 364
MS.gene067382.t1 AT3G21240 40.185 540 286 12 34 561 33 547 1.56e-116 357
MS.gene067382.t1 AT5G38120 40.127 471 262 8 28 495 24 477 7.39e-110 337
MS.gene067382.t1 AT5G38120 40.127 471 262 8 28 495 24 477 7.39e-110 337
MS.gene067382.t1 AT1G51680 41.211 512 279 11 59 561 56 554 2.34e-107 333
MS.gene067382.t1 AT3G21230 39.024 533 299 8 41 561 43 561 2.46e-107 333
MS.gene067382.t1 AT1G65060 38.340 506 295 6 59 561 66 557 7.20e-101 317
MS.gene067382.t1 AT3G21230 38.492 504 284 8 41 532 43 532 8.23e-96 303
MS.gene067382.t1 AT1G51680 40.373 483 266 11 59 532 56 525 1.75e-94 299
MS.gene067382.t1 AT1G62940 36.346 520 288 9 58 562 43 534 8.95e-93 295
MS.gene067382.t1 AT1G20490 39.573 422 234 7 28 444 23 428 1.08e-92 291
MS.gene067382.t1 AT1G20490 39.573 422 234 7 28 444 23 428 1.08e-92 291
MS.gene067382.t1 AT3G21240 38.655 476 255 12 34 497 33 483 1.32e-91 290
MS.gene067382.t1 AT3G21240 38.655 476 255 12 34 497 33 483 1.32e-91 290
MS.gene067382.t1 AT3G21230 37.740 469 266 8 41 497 43 497 1.96e-85 274
MS.gene067382.t1 AT1G51680 39.286 448 250 11 59 497 56 490 4.73e-83 268
MS.gene067382.t1 AT1G65060 35.545 422 255 6 59 477 66 473 1.73e-69 232
MS.gene067382.t1 AT3G21230 32.663 398 242 8 41 426 43 426 4.47e-48 174
MS.gene067382.t1 AT1G77240 29.944 531 310 21 68 566 43 543 1.42e-42 160
MS.gene067382.t1 AT3G48990 27.108 498 333 14 68 558 30 504 2.61e-42 159
MS.gene067382.t1 AT1G21540 27.068 532 328 16 68 566 44 548 3.22e-41 157
MS.gene067382.t1 AT1G65880 27.985 536 324 18 67 562 38 551 1.07e-39 153
MS.gene067382.t1 AT5G16370 27.168 519 325 17 72 562 46 539 3.10e-38 148
MS.gene067382.t1 AT1G65890 26.972 545 317 18 67 562 38 550 7.10e-38 147
MS.gene067382.t1 AT1G21530 27.736 530 318 21 68 562 44 543 7.72e-38 147
MS.gene067382.t1 AT1G21530 27.632 532 314 23 68 562 44 541 1.07e-37 147
MS.gene067382.t1 AT1G20560 28.740 508 315 17 81 562 54 540 4.11e-36 142
MS.gene067382.t1 AT1G66120 28.906 512 314 16 81 562 52 543 1.05e-35 141
MS.gene067382.t1 AT1G75960 27.668 506 321 15 81 561 53 538 1.29e-35 140
MS.gene067382.t1 AT3G16910 29.517 393 236 10 194 562 181 556 4.49e-35 139
MS.gene067382.t1 AT5G16340 26.718 524 321 18 72 562 46 539 2.48e-34 137
MS.gene067382.t1 AT1G30520 27.289 546 296 19 78 562 42 547 8.18e-34 135
MS.gene067382.t1 AT1G30520 27.289 546 296 19 78 562 42 547 8.18e-34 135
MS.gene067382.t1 AT1G20560 31.579 380 227 14 198 561 99 461 3.13e-33 133
MS.gene067382.t1 AT2G17650 27.984 511 325 16 81 562 99 595 2.11e-32 132
MS.gene067382.t1 AT5G36880 25.516 533 355 14 68 568 197 719 2.45e-30 126
MS.gene067382.t1 AT5G36880 25.328 533 356 14 68 568 147 669 2.87e-30 126
MS.gene067382.t1 AT5G36880 25.328 533 356 14 68 568 147 669 2.87e-30 126
MS.gene067382.t1 AT5G36880 25.328 533 356 14 68 568 147 669 2.87e-30 126
MS.gene067382.t1 AT5G36880 25.328 533 356 14 68 568 147 669 2.87e-30 126
MS.gene067382.t1 AT3G16170 26.440 382 237 13 210 561 234 601 2.05e-29 123
MS.gene067382.t1 AT5G27600 26.527 524 303 16 24 481 60 567 2.76e-26 114
MS.gene067382.t1 AT1G30520 26.464 461 251 16 78 492 42 460 1.27e-25 110
MS.gene067382.t1 AT1G30520 26.464 461 251 16 78 492 42 460 1.27e-25 110
MS.gene067382.t1 AT5G36880 26.039 457 299 13 68 495 147 593 2.03e-24 108
MS.gene067382.t1 AT1G76290 26.172 512 315 21 81 561 52 531 1.69e-23 104
MS.gene067382.t1 AT1G68270 36.709 158 89 2 414 561 363 519 2.11e-23 104
MS.gene067382.t1 AT1G64400 24.673 535 313 19 22 483 21 538 1.08e-22 102
MS.gene067382.t1 AT1G77590 26.943 386 197 14 211 531 100 465 8.85e-22 99.8
MS.gene067382.t1 AT1G77590 26.943 386 197 14 211 531 246 611 1.49e-21 99.4
MS.gene067382.t1 AT4G11030 22.814 526 318 16 52 509 58 563 4.90e-21 97.8
MS.gene067382.t1 AT3G05970 27.360 519 299 18 27 481 63 567 2.71e-20 95.5
MS.gene067382.t1 AT4G23850 25.240 416 232 13 141 509 180 563 4.87e-20 94.7
MS.gene067382.t1 AT2G04350 22.649 521 313 16 70 518 131 633 1.24e-14 77.4
MS.gene067382.t1 AT2G04350 22.649 521 313 16 70 518 131 633 1.24e-14 77.4
MS.gene067382.t1 AT3G23790 34.247 146 80 5 348 481 465 606 5.43e-14 75.5

Find 122 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTGGTCTTCATAAAA+TGG 0.181069 5.2:-18257616 MS.gene067382:CDS
ATGGGATAGTAAATTGAATT+TGG 0.204514 5.2:+18257558 None:intergenic
AAGGAAACAACATCGATAAA+AGG 0.225166 5.2:+18257738 None:intergenic
GCCCTTATTGATTCCTCATC+TGG 0.228419 5.2:-18257684 MS.gene067382:CDS
ATGACATTGATGAGGCTATT+AGG 0.229682 5.2:-18256987 MS.gene067382:CDS
TATCAAATACAAGGGCTTTC+AGG 0.237687 5.2:-18255927 MS.gene067382:intron
TTGCCGATGTTCCATTTATA+TGG 0.273927 5.2:-18257072 MS.gene067382:CDS
ACCTGTATAAAATCAACATT+AGG 0.288055 5.2:+18256793 None:intergenic
TTAGTCAAATCCTTGGCTTC+TGG 0.296472 5.2:-18257630 MS.gene067382:CDS
TGCTACCGGATTATTGTCAT+TGG 0.301910 5.2:-18257037 MS.gene067382:CDS
ATAAAGAAGAATCTTATTTA+CGG 0.307107 5.2:-18257291 MS.gene067382:CDS
AGAGCGATAAACCATATAAA+TGG 0.308944 5.2:+18257061 None:intergenic
AAAGCTCACCGCTGAGGCCT+GGG 0.310423 5.2:+18256307 None:intergenic
CAAAGCTCACCGCTGAGGCC+TGG 0.333831 5.2:+18256306 None:intergenic
ACGGCGGCATCAACTATTTC+AGG 0.342047 5.2:+18255757 None:intergenic
ACTTTCCGAACCTTCTTGTA+TGG 0.345292 5.2:+18255401 None:intergenic
CAGGCCTCAGCGGTGAGCTT+TGG 0.348099 5.2:-18256305 MS.gene067382:CDS
AGCAATTCCTGAGTTGCTTT+CGG 0.356180 5.2:+18255333 None:intergenic
GATGGATATTTATATTTGTC+TGG 0.362112 5.2:-18255965 MS.gene067382:CDS
TAACAAATTTGAGTAAGAAA+TGG 0.376318 5.2:+18257656 None:intergenic
TGACATTGATGAGGCTATTA+GGG 0.377552 5.2:-18256986 MS.gene067382:CDS
GCTGGTATACTATATTCTTC+TGG 0.393544 5.2:-18257222 MS.gene067382:CDS
GTGTCTTCTTGTTTAATAAC+CGG 0.393788 5.2:+18257246 None:intergenic
GGAGATTCCAGTGGCATTTG+TGG 0.394931 5.2:-18255617 MS.gene067382:CDS
AATAGAATTTACCATAAGTA+AGG 0.398034 5.2:+18257316 None:intergenic
AGGGTAAGTGATAGTGCTAT+TGG 0.400607 5.2:+18257827 None:intergenic
TCCCAGATGAGGAATCAATA+AGG 0.401871 5.2:+18257682 None:intergenic
GGGTAAGTGATAGTGCTATT+GGG 0.410599 5.2:+18257828 None:intergenic
TGCTGTGCCAGAAAATGAAA+TGG 0.427789 5.2:-18257373 MS.gene067382:CDS
GCTTCTGGTCTTCATAAAAT+GGG 0.430989 5.2:-18257615 MS.gene067382:CDS
GTCAATGCCGACAACATCAT+TGG 0.431399 5.2:+18256925 None:intergenic
CCCTTATTGATTCCTCATCT+GGG 0.431410 5.2:-18257683 MS.gene067382:CDS
TGGAGGCAAAAGTGGTAGAT+TGG 0.438591 5.2:-18256350 MS.gene067382:CDS
CCTGTATAAAATCAACATTA+GGG 0.441453 5.2:+18256794 None:intergenic
AAAGATGGTTGGCTACATAC+TGG 0.442873 5.2:-18256010 MS.gene067382:CDS
GATCGATTACGTTGCTAAGC+AGG 0.446575 5.2:-18255557 MS.gene067382:intron
GCTACCGGATTATTGTCATT+GGG 0.459661 5.2:-18257036 MS.gene067382:CDS
AGTGGCAAGGTTGATGAAGC+TGG 0.461000 5.2:-18255640 MS.gene067382:CDS
TGTTTAATAACCGGCCTTTG+AGG 0.466941 5.2:+18257255 None:intergenic
AGCGGTGAGCTTTGGTTGAG+TGG 0.470188 5.2:-18256297 MS.gene067382:CDS
AATCAACATTAGGGAAAGCT+TGG 0.471169 5.2:+18256803 None:intergenic
GCACTATCACTTACCCTCAT+TGG 0.472078 5.2:-18257821 MS.gene067382:CDS
TGTTGTTCCAATGATGTTGT+CGG 0.475822 5.2:-18256932 MS.gene067382:CDS
ATAACACTCAATAGTACAAT+GGG 0.478275 5.2:+18257540 None:intergenic
CTTTGATTTGCCTCAAAGGC+CGG 0.478557 5.2:-18257265 MS.gene067382:CDS
TTTCACAACTATTCCTCACT+AGG 0.480714 5.2:-18256390 MS.gene067382:CDS
CTATATTCTTCTGGTACTAC+AGG 0.481197 5.2:-18257213 MS.gene067382:CDS
GGTTTATCGCTCTTTGCTAC+CGG 0.483340 5.2:-18257051 MS.gene067382:CDS
AAGCTCACCGCTGAGGCCTG+GGG 0.483974 5.2:+18256308 None:intergenic
TTCTTGACCACAAATGCCAC+TGG 0.484108 5.2:+18255610 None:intergenic
GCGGTGAGCTTTGGTTGAGT+GGG 0.484393 5.2:-18256296 MS.gene067382:CDS
CCCAGATGAGGAATCAATAA+GGG 0.484993 5.2:+18257683 None:intergenic
ATTTGATAATGTCTTTCAAA+CGG 0.487340 5.2:+18255942 None:intergenic
TCATAGAAATCTTATTGCTA+TGG 0.487900 5.2:-18257166 MS.gene067382:CDS
GTTAACTTTAGTCAAATCCT+TGG 0.487963 5.2:-18257637 MS.gene067382:CDS
AACAAAGCTTCTAAATCAGC+AGG 0.490080 5.2:+18255790 None:intergenic
ACAGCAGTTTACAATCTCCT+AGG 0.491169 5.2:-18257776 MS.gene067382:CDS
ATTTCGCTAACACTACTAAG+AGG 0.492190 5.2:+18257486 None:intergenic
AACAGCTAGATGCACAGTCT+TGG 0.496252 5.2:+18257095 None:intergenic
TTGCTCCATACAAGAAGGTT+CGG 0.499371 5.2:-18255406 MS.gene067382:CDS
CTATTGAGTGTTATGTATCT+AGG 0.499995 5.2:-18257531 MS.gene067382:CDS
TGTTAGCGAAATTCGTAAGC+AGG 0.502850 5.2:-18257475 MS.gene067382:CDS
CGATAAAAGGGTTGGTCGGA+AGG 0.502966 5.2:+18257751 None:intergenic
AAAGTGGTAGATTGGAAAAG+TGG 0.504015 5.2:-18256342 MS.gene067382:CDS
CATAGGATACTTGAATAATG+AGG 0.506502 5.2:-18256056 MS.gene067382:intron
ATCAAGAAGTTAGAGTCATT+AGG 0.508091 5.2:-18257408 MS.gene067382:CDS
GGGTTGGTCGGAAGGTTCCT+AGG 0.509288 5.2:+18257759 None:intergenic
GGTTATGGAATGACTGAGTC+GGG 0.510519 5.2:-18256447 MS.gene067382:CDS
AGGAAACAACATCGATAAAA+GGG 0.516631 5.2:+18257739 None:intergenic
GAAAGACATTATCAAATACA+AGG 0.519394 5.2:-18255936 MS.gene067382:CDS
ATGTCAACAATTGATAAAGA+TGG 0.520159 5.2:-18256025 MS.gene067382:CDS
ACACTACTAAGAGGATTCAA+TGG 0.522819 5.2:+18257495 None:intergenic
TTCATATACTTACGCTGTAA+CGG 0.523614 5.2:+18255738 None:intergenic
TTTATAGCTCCTTTGCTCAA+AGG 0.526141 5.2:+18256835 None:intergenic
GTGGCAAGGTTGATGAAGCT+GGG 0.529856 5.2:-18255639 MS.gene067382:CDS
GTTCTACAATCGTTGTAATG+AGG 0.530238 5.2:-18257014 MS.gene067382:CDS
AAATCCTCCGAAAGCAACTC+AGG 0.535730 5.2:-18255340 MS.gene067382:CDS
TCCAAACATGGAGGCAAAAG+TGG 0.536470 5.2:-18256358 MS.gene067382:CDS
CATAAAATGGGTGTTTCACA+AGG 0.537350 5.2:-18257603 MS.gene067382:CDS
TTCACAACTATTCCTCACTA+GGG 0.540578 5.2:-18256389 MS.gene067382:CDS
GGGTTATGGAATGACTGAGT+CGG 0.541532 5.2:-18256448 MS.gene067382:CDS
GGTAAGTGATAGTGCTATTG+GGG 0.541720 5.2:+18257829 None:intergenic
GGTGCTGAGCCTTTGAGCAA+AGG 0.542374 5.2:-18256844 MS.gene067382:CDS
ATACAAGAAGGTTCGGAAAG+TGG 0.543810 5.2:-18255399 MS.gene067382:CDS
GTGACTTTGATTTGCCTCAA+AGG 0.544155 5.2:-18257269 MS.gene067382:CDS
AGAACCCAATGACAATAATC+CGG 0.547525 5.2:+18257032 None:intergenic
ATGACTGAGTCGGGCGCAGT+AGG 0.553182 5.2:-18256438 MS.gene067382:CDS
AGTGGGCCTTCAATTATGAA+AGG 0.573787 5.2:-18256279 MS.gene067382:intron
CAACAATTGATAAAGATGGT+TGG 0.574233 5.2:-18256021 MS.gene067382:CDS
CATAACACTCAATAGTACAA+TGG 0.577708 5.2:+18257539 None:intergenic
AACAACATCGATAAAAGGGT+TGG 0.578041 5.2:+18257743 None:intergenic
AAGATACCGAGATCTGCGAC+TGG 0.579224 5.2:-18255365 MS.gene067382:CDS
AGGGCTAAAACCCCGTCATG+AGG 0.582643 5.2:+18257702 None:intergenic
AGCTCACCGCTGAGGCCTGG+GGG 0.583783 5.2:+18256309 None:intergenic
AAAGACATTATCAAATACAA+GGG 0.586533 5.2:-18255935 MS.gene067382:CDS
AAACCATATAAATGGAACAT+CGG 0.588130 5.2:+18257069 None:intergenic
AATTCCTGAGTTGCTTTCGG+AGG 0.588562 5.2:+18255336 None:intergenic
TTTATGAAGACCAGAAGCCA+AGG 0.591784 5.2:+18257620 None:intergenic
GCATTTGTGGTCAAGAAAGT+CGG 0.593489 5.2:-18255604 MS.gene067382:CDS
ATATACTTACGCTGTAACGG+CGG 0.594057 5.2:+18255741 None:intergenic
GGCATTGACAGCGAAGGCAA+AGG 0.603341 5.2:-18256911 MS.gene067382:CDS
CGTAAGCAGGTTAAAGAGTG+TGG 0.604728 5.2:-18257462 MS.gene067382:CDS
AGTTTAAGACATGTCTCTTG+CGG 0.607812 5.2:-18256865 MS.gene067382:CDS
AGGGTTGTTAGCTCCAAACA+TGG 0.614345 5.2:-18256370 MS.gene067382:CDS
GGTACTACAGGTGTGAGCAA+AGG 0.618393 5.2:-18257201 MS.gene067382:CDS
TTGACAGCGAAGGCAAAGGA+TGG 0.620619 5.2:-18256907 MS.gene067382:CDS
ATTAAACAAGAAGACACTGC+TGG 0.621260 5.2:-18257240 MS.gene067382:CDS
ACATCGATAAAAGGGTTGGT+CGG 0.626589 5.2:+18257747 None:intergenic
GGGCTAAAACCCCGTCATGA+GGG 0.628723 5.2:+18257703 None:intergenic
AGATACCGAGATCTGCGACT+GGG 0.631075 5.2:-18255364 MS.gene067382:CDS
GTAGGTTGCTCCATACAAGA+AGG 0.636951 5.2:-18255411 MS.gene067382:intron
AGAAATGGAAGTCCCAGATG+AGG 0.641379 5.2:+18257671 None:intergenic
TGGCAAGGTTGATGAAGCTG+GGG 0.643596 5.2:-18255638 MS.gene067382:CDS
TGGAGCTAACAACCCTAGTG+AGG 0.644923 5.2:+18256377 None:intergenic
GAGGAAATATGACATTGATG+AGG 0.657137 5.2:-18256995 MS.gene067382:CDS
GTTGTCGGCATTGACAGCGA+AGG 0.659388 5.2:-18256917 MS.gene067382:CDS
TCAACCAAAGCTCACCGCTG+AGG 0.662229 5.2:+18256301 None:intergenic
TGCTCGACTTCAAAGCTCTG+AGG 0.675541 5.2:-18255314 None:intergenic
AGGATTCAATGGTGTAACAA+CGG 0.680938 5.2:+18257506 None:intergenic
TCGGGCGCAGTAGGAACACG+CGG 0.689165 5.2:-18256429 MS.gene067382:CDS
TGAAGCTGGGGAGATTCCAG+TGG 0.700734 5.2:-18255626 MS.gene067382:CDS
GTTGTTAGCTCCAAACATGG+AGG 0.741487 5.2:-18256367 MS.gene067382:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATCAGTATTTTTACA+AGG - chr5.2:18256919-18256938 MS.gene067382:CDS 15.0%
!!! AAATTTTTCCTTTATTTCAT+AGG - chr5.2:18257101-18257120 MS.gene067382:CDS 15.0%
!!! ATAAAGAAGAATCTTATTTA+CGG - chr5.2:18255883-18255902 MS.gene067382:intron 15.0%
!!! ATGAAATAAAGGAAAAATTT+TGG + chr5.2:18257101-18257120 None:intergenic 15.0%
!!! TTTTAAACCTTTTTTTTTTC+TGG - chr5.2:18257741-18257760 MS.gene067382:CDS 15.0%
!! AAAGACATTATCAAATACAA+GGG - chr5.2:18257239-18257258 MS.gene067382:CDS 20.0%
!! AATAGAATTTACCATAAGTA+AGG + chr5.2:18255861-18255880 None:intergenic 20.0%
!! TAACAAATTTGAGTAAGAAA+TGG + chr5.2:18255521-18255540 None:intergenic 20.0%
!!! ATTGTTTATTTTGATCAAGA+TGG - chr5.2:18257191-18257210 MS.gene067382:CDS 20.0%
!!! ATTTGATAATGTCTTTCAAA+CGG + chr5.2:18257235-18257254 None:intergenic 20.0%
!!! TGTATGATTGTTTCTTTTTA+GGG - chr5.2:18256706-18256725 MS.gene067382:intron 20.0%
!!! TGTTTTTATAACCTTACTTA+TGG - chr5.2:18255847-18255866 MS.gene067382:intron 20.0%
!!! TTTTGGTTAAGAATTTCAAA+TGG + chr5.2:18257084-18257103 None:intergenic 20.0%
!!! TTTTTACCTTTCATAATTGA+AGG + chr5.2:18256904-18256923 None:intergenic 20.0%
! AAACCATATAAATGGAACAT+CGG + chr5.2:18256108-18256127 None:intergenic 25.0%
! ACCTGTATAAAATCAACATT+AGG + chr5.2:18256384-18256403 None:intergenic 25.0%
! AGTTGAACTTTACTTGTTTA+TGG - chr5.2:18256644-18256663 MS.gene067382:intron 25.0%
! ATAACACTCAATAGTACAAT+GGG + chr5.2:18255637-18255656 None:intergenic 25.0%
! ATGGGATAGTAAATTGAATT+TGG + chr5.2:18255619-18255638 None:intergenic 25.0%
! ATGTCAACAATTGATAAAGA+TGG - chr5.2:18257149-18257168 MS.gene067382:CDS 25.0%
! CAAGTAAAGTTCAACTATTA+TGG + chr5.2:18256640-18256659 None:intergenic 25.0%
! CCTGTATAAAATCAACATTA+GGG + chr5.2:18256383-18256402 None:intergenic 25.0%
! GAAAGACATTATCAAATACA+AGG - chr5.2:18257238-18257257 MS.gene067382:CDS 25.0%
! GATGGATATTTATATTTGTC+TGG - chr5.2:18257209-18257228 MS.gene067382:CDS 25.0%
! TCAAGTATCCTATGAAATAA+AGG + chr5.2:18257112-18257131 None:intergenic 25.0%
! TCATAGAAATCTTATTGCTA+TGG - chr5.2:18256008-18256027 MS.gene067382:CDS 25.0%
! TGTGAATATGAATACCATAA+CGG + chr5.2:18256605-18256624 None:intergenic 25.0%
!! CTAATTTTCTTTTTTCTCCA+AGG + chr5.2:18256413-18256432 None:intergenic 25.0%
!! CTCTAACTTCTTGATATTTT+CGG + chr5.2:18255761-18255780 None:intergenic 25.0%
!! TCTAACTTCTTGATATTTTC+GGG + chr5.2:18255760-18255779 None:intergenic 25.0%
!! TTCAAATCCATTTCATTTTC+TGG + chr5.2:18255811-18255830 None:intergenic 25.0%
!!! AAACCTTTTTTTTTTCTGGT+AGG - chr5.2:18257745-18257764 MS.gene067382:CDS 25.0%
!!! ATTGTTTCTTTTTAGGGTTA+TGG - chr5.2:18256712-18256731 MS.gene067382:intron 25.0%
!!! CTGTATGATTGTTTCTTTTT+AGG - chr5.2:18256705-18256724 MS.gene067382:intron 25.0%
!!! GTTGATTTCCTAGATTTTTA+AGG - chr5.2:18256674-18256693 MS.gene067382:intron 25.0%
!!! TCATATTTTAATGTCCAAAG+AGG + chr5.2:18257486-18257505 None:intergenic 25.0%
!!! TTTTCTTTGTAATTGCTGTT+AGG - chr5.2:18256488-18256507 MS.gene067382:intron 25.0%
AAGGAAACAACATCGATAAA+AGG + chr5.2:18255439-18255458 None:intergenic 30.0%
AATCAATCTCATGCAAATGT+AGG + chr5.2:18256970-18256989 None:intergenic 30.0%
AGAGCGATAAACCATATAAA+TGG + chr5.2:18256116-18256135 None:intergenic 30.0%
AGGAAACAACATCGATAAAA+GGG + chr5.2:18255438-18255457 None:intergenic 30.0%
AGTGTCTATTACTATTGGAA+TGG - chr5.2:18257710-18257729 MS.gene067382:CDS 30.0%
ATCAAGAAGTTAGAGTCATT+AGG - chr5.2:18255766-18255785 MS.gene067382:CDS 30.0%
CAACAATTGATAAAGATGGT+TGG - chr5.2:18257153-18257172 MS.gene067382:CDS 30.0%
CAACCTACCAGAAAAAAAAA+AGG + chr5.2:18257751-18257770 None:intergenic 30.0%
CATAACACTCAATAGTACAA+TGG + chr5.2:18255638-18255657 None:intergenic 30.0%
CATAGGATACTTGAATAATG+AGG - chr5.2:18257118-18257137 MS.gene067382:CDS 30.0%
CTATTGAGTGTTATGTATCT+AGG - chr5.2:18255643-18255662 MS.gene067382:intron 30.0%
CTTCACATCCTTAAAAATCT+AGG + chr5.2:18256685-18256704 None:intergenic 30.0%
GTCATAGTGTCTATTACTAT+TGG - chr5.2:18257705-18257724 MS.gene067382:CDS 30.0%
GTTAACTTTAGTCAAATCCT+TGG - chr5.2:18255537-18255556 MS.gene067382:intron 30.0%
TTCATATACTTACGCTGTAA+CGG + chr5.2:18257439-18257458 None:intergenic 30.0%
TTCATATTCACAACATGTTC+TGG - chr5.2:18256613-18256632 MS.gene067382:intron 30.0%
TTTATACAGGTAAAGAACCT+TGG - chr5.2:18256393-18256412 MS.gene067382:CDS 30.0%
! GTGTCTTCTTGTTTAATAAC+CGG + chr5.2:18255931-18255950 None:intergenic 30.0%
! TGACTTGCGTTCTTTAATTA+AGG + chr5.2:18256436-18256455 None:intergenic 30.0%
!! TTTTTGGTGAATACCAATGA+GGG + chr5.2:18255369-18255388 None:intergenic 30.0%
!!! CCCTAATGTTGATTTTATAC+AGG - chr5.2:18256380-18256399 MS.gene067382:CDS 30.0%
AAAGTGGTAGATTGGAAAAG+TGG - chr5.2:18256832-18256851 MS.gene067382:CDS 35.0%
AACAAAGCTTCTAAATCAGC+AGG + chr5.2:18257387-18257406 None:intergenic 35.0%
AACAACATCGATAAAAGGGT+TGG + chr5.2:18255434-18255453 None:intergenic 35.0%
AATCAACATTAGGGAAAGCT+TGG + chr5.2:18256374-18256393 None:intergenic 35.0%
AATGGAATAGCAAAAGAGAC+AGG + chr5.2:18257066-18257085 None:intergenic 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr5.2:18255682-18255701 None:intergenic 35.0%
AGAACCCAATGACAATAATC+CGG + chr5.2:18256145-18256164 None:intergenic 35.0%
AGGATTCAATGGTGTAACAA+CGG + chr5.2:18255671-18255690 None:intergenic 35.0%
ATGACATTGATGAGGCTATT+AGG - chr5.2:18256187-18256206 MS.gene067382:intron 35.0%
ATGAGGGTGAGAAAAAATGA+AGG + chr5.2:18255458-18255477 None:intergenic 35.0%
ATTAAACAAGAAGACACTGC+TGG - chr5.2:18255934-18255953 MS.gene067382:CDS 35.0%
ATTTCGCTAACACTACTAAG+AGG + chr5.2:18255691-18255710 None:intergenic 35.0%
CATAAAATGGGTGTTTCACA+AGG - chr5.2:18255571-18255590 MS.gene067382:CDS 35.0%
CATTGGTATTCACCAAAAAC+TGG - chr5.2:18255370-18255389 MS.gene067382:CDS 35.0%
CGAAAATGATGTGATACTCA+AGG + chr5.2:18257509-18257528 None:intergenic 35.0%
CTATATTCTTCTGGTACTAC+AGG - chr5.2:18255961-18255980 MS.gene067382:CDS 35.0%
GAGGAAATATGACATTGATG+AGG - chr5.2:18256179-18256198 MS.gene067382:intron 35.0%
GCTGGTATACTATATTCTTC+TGG - chr5.2:18255952-18255971 MS.gene067382:CDS 35.0%
GTTAGGAGCTAAATAAATGC+TGG - chr5.2:18256505-18256524 MS.gene067382:intron 35.0%
GTTCTACAATCGTTGTAATG+AGG - chr5.2:18256160-18256179 MS.gene067382:intron 35.0%
TATCAAATACAAGGGCTTTC+AGG - chr5.2:18257247-18257266 MS.gene067382:CDS 35.0%
TGACATTGATGAGGCTATTA+GGG - chr5.2:18256188-18256207 MS.gene067382:intron 35.0%
TGTTGTTCCAATGATGTTGT+CGG - chr5.2:18256242-18256261 MS.gene067382:intron 35.0%
TTCACAACTATTCCTCACTA+GGG - chr5.2:18256785-18256804 MS.gene067382:intron 35.0%
TTGCCGATGTTCCATTTATA+TGG - chr5.2:18256102-18256121 MS.gene067382:intron 35.0%
TTTATAGCTCCTTTGCTCAA+AGG + chr5.2:18256342-18256361 None:intergenic 35.0%
TTTCACAACTATTCCTCACT+AGG - chr5.2:18256784-18256803 MS.gene067382:intron 35.0%
! AAAAAGGTACAAGTAGGCTA+AGG + chr5.2:18257041-18257060 None:intergenic 35.0%
! AGTTTAAGACATGTCTCTTG+CGG - chr5.2:18256309-18256328 MS.gene067382:CDS 35.0%
! CACATCATTTTCGATATCAG+TGG - chr5.2:18257516-18257535 MS.gene067382:CDS 35.0%
! CTATGGTTGAGCTTTTTATG+AGG - chr5.2:18256025-18256044 MS.gene067382:CDS 35.0%
! CTGCTGTAAATTCCAGTTTT+TGG + chr5.2:18255385-18255404 None:intergenic 35.0%
! GCTTCTGGTCTTCATAAAAT+GGG - chr5.2:18255559-18255578 MS.gene067382:CDS 35.0%
!!! ATTTTTTCTCACCCTCATGA+CGG - chr5.2:18255460-18255479 MS.gene067382:intron 35.0%
!!! GTTTTTGGTGAATACCAATG+AGG + chr5.2:18255370-18255389 None:intergenic 35.0%
ACAGCAGTTTACAATCTCCT+AGG - chr5.2:18255398-18255417 MS.gene067382:CDS 40.0%
ACTTTCCGAACCTTCTTGTA+TGG + chr5.2:18257776-18257795 None:intergenic 40.0%
AGTGGGCCTTCAATTATGAA+AGG - chr5.2:18256895-18256914 MS.gene067382:CDS 40.0%
ATACAAGAAGGTTCGGAAAG+TGG - chr5.2:18257775-18257794 MS.gene067382:CDS 40.0%
ATATACTTACGCTGTAACGG+CGG + chr5.2:18257436-18257455 None:intergenic 40.0%
CCCAGATGAGGAATCAATAA+GGG + chr5.2:18255494-18255513 None:intergenic 40.0%
GCAAAAGAGACAGGCAAAAA+AGG + chr5.2:18257057-18257076 None:intergenic 40.0%
GCATTTGTGGTCAAGAAAGT+CGG - chr5.2:18257570-18257589 MS.gene067382:CDS 40.0%
GCTACCGGATTATTGTCATT+GGG - chr5.2:18256138-18256157 MS.gene067382:intron 40.0%
TCCCAGATGAGGAATCAATA+AGG + chr5.2:18255495-18255514 None:intergenic 40.0%
TGCTACCGGATTATTGTCAT+TGG - chr5.2:18256137-18256156 MS.gene067382:intron 40.0%
TGCTGTGCCAGAAAATGAAA+TGG - chr5.2:18255801-18255820 MS.gene067382:intron 40.0%
TGTTAGCGAAATTCGTAAGC+AGG - chr5.2:18255699-18255718 MS.gene067382:intron 40.0%
TGTTTAATAACCGGCCTTTG+AGG + chr5.2:18255922-18255941 None:intergenic 40.0%
TTAGTCAAATCCTTGGCTTC+TGG - chr5.2:18255544-18255563 MS.gene067382:intron 40.0%
TTGCTCCATACAAGAAGGTT+CGG - chr5.2:18257768-18257787 MS.gene067382:CDS 40.0%
TTTATGAAGACCAGAAGCCA+AGG + chr5.2:18255557-18255576 None:intergenic 40.0%
! AAAGATGGTTGGCTACATAC+TGG - chr5.2:18257164-18257183 MS.gene067382:CDS 40.0%
! ACACTATTTCTTCGCCGTTA+TGG - chr5.2:18256588-18256607 MS.gene067382:intron 40.0%
! ACATCGATAAAAGGGTTGGT+CGG + chr5.2:18255430-18255449 None:intergenic 40.0%
! AGCAATTCCTGAGTTGCTTT+CGG + chr5.2:18257844-18257863 None:intergenic 40.0%
! AGGGTAAGTGATAGTGCTAT+TGG + chr5.2:18255350-18255369 None:intergenic 40.0%
! CAGGCAAAAAAGGTACAAGT+AGG + chr5.2:18257047-18257066 None:intergenic 40.0%
! CATTTTCGATATCAGTGGCA+AGG - chr5.2:18257521-18257540 MS.gene067382:CDS 40.0%
! GGCTTCTGGTCTTCATAAAA+TGG - chr5.2:18255558-18255577 MS.gene067382:CDS 40.0%
! GGGTAAGTGATAGTGCTATT+GGG + chr5.2:18255349-18255368 None:intergenic 40.0%
! GGTAAGTGATAGTGCTATTG+GGG + chr5.2:18255348-18255367 None:intergenic 40.0%
!! CCCTTATTGATTCCTCATCT+GGG - chr5.2:18255491-18255510 MS.gene067382:intron 40.0%
!! GTGACTTTGATTTGCCTCAA+AGG - chr5.2:18255905-18255924 MS.gene067382:intron 40.0%
!! TGTCTTCTAAAGTGCCTCTT+TGG - chr5.2:18257469-18257488 MS.gene067382:CDS 40.0%
!! TTTTCTGGCACAGCAATAAC+AGG + chr5.2:18255796-18255815 None:intergenic 40.0%
!!! TTTTTTCTCACCCTCATGAC+GGG - chr5.2:18255461-18255480 MS.gene067382:intron 40.0%
AAATCCTCCGAAAGCAACTC+AGG - chr5.2:18257834-18257853 MS.gene067382:CDS 45.0%
AACAGCTAGATGCACAGTCT+TGG + chr5.2:18256082-18256101 None:intergenic 45.0%
AGAAATGGAAGTCCCAGATG+AGG + chr5.2:18255506-18255525 None:intergenic 45.0%
AGGGTTGTTAGCTCCAAACA+TGG - chr5.2:18256804-18256823 MS.gene067382:CDS 45.0%
CGTAAGCAGGTTAAAGAGTG+TGG - chr5.2:18255712-18255731 MS.gene067382:intron 45.0%
GATCGATTACGTTGCTAAGC+AGG - chr5.2:18257617-18257636 MS.gene067382:CDS 45.0%
GCACTATCACTTACCCTCAT+TGG - chr5.2:18255353-18255372 MS.gene067382:CDS 45.0%
GGGTTATGGAATGACTGAGT+CGG - chr5.2:18256726-18256745 MS.gene067382:intron 45.0%
GGTTATGGAATGACTGAGTC+GGG - chr5.2:18256727-18256746 MS.gene067382:intron 45.0%
GGTTTATCGCTCTTTGCTAC+CGG - chr5.2:18256123-18256142 MS.gene067382:intron 45.0%
GTAGGTTGCTCCATACAAGA+AGG - chr5.2:18257763-18257782 MS.gene067382:CDS 45.0%
GTCAATGCCGACAACATCAT+TGG + chr5.2:18256252-18256271 None:intergenic 45.0%
GTTGTTAGCTCCAAACATGG+AGG - chr5.2:18256807-18256826 MS.gene067382:CDS 45.0%
TCCAAACATGGAGGCAAAAG+TGG - chr5.2:18256816-18256835 MS.gene067382:CDS 45.0%
TGGAGGCAAAAGTGGTAGAT+TGG - chr5.2:18256824-18256843 MS.gene067382:CDS 45.0%
TTCTTGACCACAAATGCCAC+TGG + chr5.2:18257567-18257586 None:intergenic 45.0%
! AATTCCTGAGTTGCTTTCGG+AGG + chr5.2:18257841-18257860 None:intergenic 45.0%
! ATTTTCCCAGTCGCAGATCT+CGG + chr5.2:18257818-18257837 None:intergenic 45.0%
! TTTTTCTCACCCTCATGACG+GGG - chr5.2:18255462-18255481 MS.gene067382:intron 45.0%
!! ACCACTTTTGCCTCCATGTT+TGG + chr5.2:18256820-18256839 None:intergenic 45.0%
!! CTTTGATTTGCCTCAAAGGC+CGG - chr5.2:18255909-18255928 MS.gene067382:intron 45.0%
!! GCCCTTATTGATTCCTCATC+TGG - chr5.2:18255490-18255509 MS.gene067382:intron 45.0%
AAGATACCGAGATCTGCGAC+TGG - chr5.2:18257809-18257828 MS.gene067382:CDS 50.0%
ACGGCGGCATCAACTATTTC+AGG + chr5.2:18257420-18257439 None:intergenic 50.0%
AGATACCGAGATCTGCGACT+GGG - chr5.2:18257810-18257829 MS.gene067382:CDS 50.0%
GGAGATTCCAGTGGCATTTG+TGG - chr5.2:18257557-18257576 MS.gene067382:CDS 50.0%
GGTACTACAGGTGTGAGCAA+AGG - chr5.2:18255973-18255992 MS.gene067382:CDS 50.0%
TGGAGCTAACAACCCTAGTG+AGG + chr5.2:18256800-18256819 None:intergenic 50.0%
TTGACAGCGAAGGCAAAGGA+TGG - chr5.2:18256267-18256286 MS.gene067382:intron 50.0%
! AGTGGTAAGTTTTTGCCCCC+AGG - chr5.2:18256850-18256869 MS.gene067382:CDS 50.0%
!! AGTGGCAAGGTTGATGAAGC+TGG - chr5.2:18257534-18257553 MS.gene067382:CDS 50.0%
!! CGATAAAAGGGTTGGTCGGA+AGG + chr5.2:18255426-18255445 None:intergenic 50.0%
!! GTGGCAAGGTTGATGAAGCT+GGG - chr5.2:18257535-18257554 MS.gene067382:CDS 50.0%
!! TGGCAAGGTTGATGAAGCTG+GGG - chr5.2:18257536-18257555 MS.gene067382:CDS 50.0%
AGGGCTAAAACCCCGTCATG+AGG + chr5.2:18255475-18255494 None:intergenic 55.0%
GGGCTAAAACCCCGTCATGA+GGG + chr5.2:18255474-18255493 None:intergenic 55.0%
TCAACCAAAGCTCACCGCTG+AGG + chr5.2:18256876-18256895 None:intergenic 55.0%
TGAAGCTGGGGAGATTCCAG+TGG - chr5.2:18257548-18257567 MS.gene067382:CDS 55.0%
! AGCGGTGAGCTTTGGTTGAG+TGG - chr5.2:18256877-18256896 MS.gene067382:CDS 55.0%
! GCGGTGAGCTTTGGTTGAGT+GGG - chr5.2:18256878-18256897 MS.gene067382:CDS 55.0%
! GGTGCTGAGCCTTTGAGCAA+AGG - chr5.2:18256330-18256349 MS.gene067382:CDS 55.0%
!! GGCATTGACAGCGAAGGCAA+AGG - chr5.2:18256263-18256282 MS.gene067382:intron 55.0%
!! GTTGTCGGCATTGACAGCGA+AGG - chr5.2:18256257-18256276 MS.gene067382:intron 55.0%
AAAGCTCACCGCTGAGGCCT+GGG + chr5.2:18256870-18256889 None:intergenic 60.0%
ATGACTGAGTCGGGCGCAGT+AGG - chr5.2:18256736-18256755 MS.gene067382:intron 60.0%
! TTTTTGCCCCCAGGCCTCAG+CGG - chr5.2:18256859-18256878 MS.gene067382:CDS 60.0%
!! GGGTTGGTCGGAAGGTTCCT+AGG + chr5.2:18255418-18255437 None:intergenic 60.0%
AAGCTCACCGCTGAGGCCTG+GGG + chr5.2:18256869-18256888 None:intergenic 65.0%
CAAAGCTCACCGCTGAGGCC+TGG + chr5.2:18256871-18256890 None:intergenic 65.0%
CAGGCCTCAGCGGTGAGCTT+TGG - chr5.2:18256869-18256888 MS.gene067382:CDS 65.0%
TCGGGCGCAGTAGGAACACG+CGG - chr5.2:18256745-18256764 MS.gene067382:intron 65.0%
AGCTCACCGCTGAGGCCTGG+GGG + chr5.2:18256868-18256887 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 18255316 18257880 18255316 ID=MS.gene067382
chr5.2 mRNA 18255316 18257880 18255316 ID=MS.gene067382.t1;Parent=MS.gene067382
chr5.2 exon 18256795 18257880 18256795 ID=MS.gene067382.t1.exon1;Parent=MS.gene067382.t1
chr5.2 CDS 18256795 18257880 18256795 ID=cds.MS.gene067382.t1;Parent=MS.gene067382.t1
chr5.2 exon 18256280 18256469 18256280 ID=MS.gene067382.t1.exon2;Parent=MS.gene067382.t1
chr5.2 CDS 18256280 18256469 18256280 ID=cds.MS.gene067382.t1;Parent=MS.gene067382.t1
chr5.2 exon 18255928 18256073 18255928 ID=MS.gene067382.t1.exon3;Parent=MS.gene067382.t1
chr5.2 CDS 18255928 18256073 18255928 ID=cds.MS.gene067382.t1;Parent=MS.gene067382.t1
chr5.2 exon 18255751 18255818 18255751 ID=MS.gene067382.t1.exon4;Parent=MS.gene067382.t1
chr5.2 CDS 18255751 18255818 18255751 ID=cds.MS.gene067382.t1;Parent=MS.gene067382.t1
chr5.2 exon 18255558 18255660 18255558 ID=MS.gene067382.t1.exon5;Parent=MS.gene067382.t1
chr5.2 CDS 18255558 18255660 18255558 ID=cds.MS.gene067382.t1;Parent=MS.gene067382.t1
chr5.2 exon 18255316 18255429 18255316 ID=MS.gene067382.t1.exon6;Parent=MS.gene067382.t1
chr5.2 CDS 18255316 18255429 18255316 ID=cds.MS.gene067382.t1;Parent=MS.gene067382.t1
Gene Sequence

>MS.gene067382

ATGGCTGCAAACTGGAATTTTTTCTCTCTCCCCAATAGCACTATCACTTACCCTCATTGGTATTCACCAAAAACTGGAATTTACAGCAGTTTACAATCTCCTAGGAACCTTCCGACCAACCCTTTTATCGATGTTGTTTCCTTCATTTTTTCTCACCCTCATGACGGGGTTTTAGCCCTTATTGATTCCTCATCTGGGACTTCCATTTCTTACTCAAATTTGTTAACTTTAGTCAAATCCTTGGCTTCTGGTCTTCATAAAATGGGTGTTTCACAAGGTGATGTTGTTTTGCTATTACTTCCAAATTCAATTTACTATCCCATTGTACTATTGAGTGTTATGTATCTAGGTGCCGTTGTTACACCATTGAATCCTCTTAGTAGTGTTAGCGAAATTCGTAAGCAGGTTAAAGAGTGTGGTGTGAGCTTTGCTTTTACTATACCCGAAAATATCAAGAAGTTAGAGTCATTAGGCATTCCTGTTATTGCTGTGCCAGAAAATGAAATGGATTTGAAGCATGATTGTTTCTCATGTTTTTATAACCTTACTTATGGTAAATTCTATTCAATAAAGAAGAATCTTATTTACGGTGACTTTGATTTGCCTCAAAGGCCGGTTATTAAACAAGAAGACACTGCTGGTATACTATATTCTTCTGGTACTACAGGTGTGAGCAAAGGAGTTGTTTTAACTCATAGAAATCTTATTGCTATGGTTGAGCTTTTTATGAGGTTTGAAGCTTCTCAATATGAATACTCTAGTTCCAAGACTGTGCATCTAGCTGTTTTGCCGATGTTCCATTTATATGGTTTATCGCTCTTTGCTACCGGATTATTGTCATTGGGTTCTACAATCGTTGTAATGAGGAAATATGACATTGATGAGGCTATTAGGGCGATTGATAAATATAAAGTTACACACTTTCATGTTGTTCCAATGATGTTGTCGGCATTGACAGCGAAGGCAAAGGATGGTAATAGAAGTAAGTTGCAGAGTTTAAGACATGTCTCTTGCGGTGCTGAGCCTTTGAGCAAAGGAGCTATAAATGATTTTGTCCAAGCTTTCCCTAATGTTGATTTTATACAGGTAAAGAACCTTGGAGAAAAAAGAAAATTAGCCTTAATTAAAGAACGCAAGTCATGCTACATCATTTATATAATTGATTGAGTTATTTTTCTTTGTAATTGCTGTTAGGAGCTAAATAAATGCTGGTTTCATTTAGTTATGAGAGAACTTGTGAACTCTAAAATAATTGTTAATAGCAGATGCTAAACACTATTTCTTCGCCGTTATGGTATTCATATTCACAACATGTTCTGGACCATAATAGTTGAACTTTACTTGTTTATGGTAAAAATGTTGATTTCCTAGATTTTTAAGGATGTGAAGCTGTATGATTGTTTCTTTTTAGGGTTATGGAATGACTGAGTCGGGCGCAGTAGGAACACGCGGCTTCAATACTGATAAGTTTCACAACTATTCCTCACTAGGGTTGTTAGCTCCAAACATGGAGGCAAAAGTGGTAGATTGGAAAAGTGGTAAGTTTTTGCCCCCAGGCCTCAGCGGTGAGCTTTGGTTGAGTGGGCCTTCAATTATGAAAGGTAAAAAATCAGTATTTTTACAAGGTCTTGTCTATTTTGTGTAATCTTAACCTACATTTGCATGAGATTGATTCATTGACTTCAACTATTTTGTTGCTATAACTTTTGTTTCTGTCTTATTCCTTAGCCTACTTGTACCTTTTTTGCCTGTCTCTTTTGCTATTCCATTTGAAATTCTTAACCAAAATTTTTCCTTTATTTCATAGGATACTTGAATAATGAGGAAGCTACAATGTCAACAATTGATAAAGATGGTTGGCTACATACTGGAGATATTGTTTATTTTGATCAAGATGGATATTTATATTTGTCTGGCCGTTTGAAAGACATTATCAAATACAAGGGCTTTCAGGTATCAAACTTTTTGTTTATTAAACTTCTATAAGAAAATGTTCTTATACAATTTATTTTTATTAAATATGTATGCATTGTTCTTATGTATGACACATGATCTTTTACAGATTGCTCCTGCTGATTTAGAAGCTTTGTTAATTTCGCATCCTGAAATAGTTGATGCCGCCGTTACAGCGTAAGTATATGAACACTTCATTTTGTCTTCTAAAGTGCCTCTTTGGACATTAAAATATGACCTTGAGTATCACATCATTTTCGATATCAGTGGCAAGGTTGATGAAGCTGGGGAGATTCCAGTGGCATTTGTGGTCAAGAAAGTCGGAAGTGTACTTTCTTCACAGCATGTGATCGATTACGTTGCTAAGCAGGTTTTTCTCTATGAGACAGACATGAAGTTCTATGATATTTCAATCGATATTACTACTAGAATCGATGTCATAGTGTCTATTACTATTGGAATGGTTTTCACATTTTAAACCTTTTTTTTTTCTGGTAGGTTGCTCCATACAAGAAGGTTCGGAAAGTGGTCTTCACAGACAAGATACCGAGATCTGCGACTGGGAAAATCCTCCGAAAGCAACTCAGGAATTGCTCGACTTCAAAGCTCTGA
Protein sequence

>MS.gene067382.t1

MAANWNFFSLPNSTITYPHWYSPKTGIYSSLQSPRNLPTNPFIDVVSFIFSHPHDGVLALIDSSSGTSISYSNLLTLVKSLASGLHKMGVSQGDVVLLLLPNSIYYPIVLLSVMYLGAVVTPLNPLSSVSEIRKQVKECGVSFAFTIPENIKKLESLGIPVIAVPENEMDLKHDCFSCFYNLTYGKFYSIKKNLIYGDFDLPQRPVIKQEDTAGILYSSGTTGVSKGVVLTHRNLIAMVELFMRFEASQYEYSSSKTVHLAVLPMFHLYGLSLFATGLLSLGSTIVVMRKYDIDEAIRAIDKYKVTHFHVVPMMLSALTAKAKDGNRSKLQSLRHVSCGAEPLSKGAINDFVQAFPNVDFIQGYGMTESGAVGTRGFNTDKFHNYSSLGLLAPNMEAKVVDWKSGKFLPPGLSGELWLSGPSIMKGYLNNEEATMSTIDKDGWLHTGDIVYFDQDGYLYLSGRLKDIIKYKGFQIAPADLEALLISHPEIVDAAVTAGKVDEAGEIPVAFVVKKVGSVLSSQHVIDYVAKQVAPYKKVRKVVFTDKIPRSATGKILRKQLRNCSTSKL