AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067378

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067378.t1 MTR_5g027480 94.054 555 33 0 1 555 1 555 0.0 1063
MS.gene067378.t1 MTR_5g027500 79.401 568 104 2 1 555 1 568 0.0 886
MS.gene067378.t1 MTR_4g077900 80.962 478 89 1 80 555 1 478 0.0 789
MS.gene067378.t1 MTR_1g026310 44.080 549 290 6 18 555 18 560 7.83e-155 454
MS.gene067378.t1 MTR_8g039720 43.915 567 294 10 5 555 1 559 3.11e-152 448
MS.gene067378.t1 MTR_1g090737 44.732 541 281 9 18 547 25 558 2.34e-144 428
MS.gene067378.t1 MTR_4g057730 41.078 538 302 7 18 547 4 534 2.29e-135 404
MS.gene067378.t1 MTR_2g095510 40.260 539 305 7 25 555 10 539 3.73e-132 396
MS.gene067378.t1 MTR_1g063120 39.162 549 303 9 20 547 6 544 1.11e-130 392
MS.gene067378.t1 MTR_3g088870 42.169 498 266 7 58 547 67 550 1.22e-128 387
MS.gene067378.t1 MTR_3g088880 41.700 494 271 7 58 547 68 548 4.51e-126 381
MS.gene067378.t1 MTR_1g063110 39.083 545 309 8 18 547 4 540 1.75e-125 379
MS.gene067378.t1 MTR_4g057730 40.592 473 266 7 18 482 4 469 1.53e-110 338
MS.gene067378.t1 MTR_1g073180 40.486 494 274 8 59 549 52 528 5.66e-110 339
MS.gene067378.t1 MTR_2g105570 39.171 531 303 10 30 548 20 542 7.43e-110 339
MS.gene067378.t1 MTR_4g128337 38.200 500 291 8 58 547 49 540 1.25e-104 325
MS.gene067378.t1 MTR_4g005750 40.832 529 291 14 30 548 19 535 2.09e-104 325
MS.gene067378.t1 MTR_4g057730 39.956 453 257 7 18 462 4 449 3.94e-102 316
MS.gene067378.t1 MTR_5g007640 38.447 515 282 9 58 553 87 585 9.74e-99 311
MS.gene067378.t1 MTR_1g073180 39.158 475 269 8 59 530 74 531 1.29e-98 309
MS.gene067378.t1 MTR_3g460770 33.938 551 320 10 26 555 14 541 4.67e-98 308
MS.gene067378.t1 MTR_2g095510 39.013 446 255 7 25 462 10 446 1.09e-97 304
MS.gene067378.t1 MTR_1g026470 37.949 390 225 6 18 396 18 401 1.28e-77 251
MS.gene067378.t1 MTR_1g026430 37.949 390 225 6 18 396 18 401 1.66e-77 250
MS.gene067378.t1 MTR_3g035130 31.006 487 314 12 68 545 32 505 2.24e-54 192
MS.gene067378.t1 MTR_3g035130 30.839 441 283 12 68 499 32 459 2.11e-43 161
MS.gene067378.t1 MTR_5g076240 28.435 524 316 17 66 549 38 542 4.06e-43 162
MS.gene067378.t1 MTR_1569s0010 26.012 519 336 12 68 549 22 529 1.48e-42 160
MS.gene067378.t1 MTR_2g030240 26.433 541 318 15 71 555 31 547 4.19e-38 147
MS.gene067378.t1 MTR_4g011790 27.456 397 255 10 182 554 211 598 6.79e-38 147
MS.gene067378.t1 MTR_0803s0020 27.397 511 293 14 70 541 263 734 2.14e-37 148
MS.gene067378.t1 MTR_4g013210 27.970 404 248 11 182 554 54 445 7.95e-37 142
MS.gene067378.t1 MTR_4g011790 27.094 406 254 12 182 554 211 607 3.76e-36 143
MS.gene067378.t1 MTR_5g076220 28.684 380 221 12 196 548 186 542 1.55e-35 140
MS.gene067378.t1 MTR_4g011790 27.273 385 247 10 182 542 211 586 1.12e-34 139
MS.gene067378.t1 MTR_3g064420 26.236 526 333 15 64 548 36 547 1.54e-34 138
MS.gene067378.t1 MTR_2g044180 27.470 506 317 16 81 548 59 552 1.99e-34 137
MS.gene067378.t1 MTR_2g044180 29.668 391 246 13 175 548 83 461 2.51e-34 135
MS.gene067378.t1 MTR_4g121600 26.255 518 332 16 69 548 47 552 8.99e-34 135
MS.gene067378.t1 MTR_2g030240 28.392 398 230 10 197 555 72 453 6.03e-33 131
MS.gene067378.t1 MTR_0161s0050 28.378 370 235 10 196 548 188 544 1.04e-32 132
MS.gene067378.t1 MTR_5g008800 28.713 505 325 16 69 549 41 534 2.06e-32 131
MS.gene067378.t1 MTR_5g008820 27.220 518 312 19 69 548 41 531 6.39e-32 130
MS.gene067378.t1 MTR_8g074040 27.308 509 305 21 80 548 94 577 9.06e-31 127
MS.gene067378.t1 MTR_8g074040 28.385 384 234 14 185 549 99 460 1.57e-30 125
MS.gene067378.t1 MTR_4g013210 25.301 498 319 14 67 523 81 566 1.24e-29 123
MS.gene067378.t1 MTR_5g076200 26.579 380 229 12 196 548 186 542 1.99e-29 122
MS.gene067378.t1 MTR_8g074120 29.223 373 223 13 198 549 137 489 6.40e-29 120
MS.gene067378.t1 MTR_7g084850 24.365 591 389 19 6 555 151 724 4.12e-28 119
MS.gene067378.t1 MTR_4g013210 26.098 387 236 10 182 554 54 404 4.42e-28 116
MS.gene067378.t1 MTR_4g013210 24.023 512 329 13 67 554 81 556 1.17e-27 117
MS.gene067378.t1 MTR_5g023730 26.692 517 325 19 69 553 48 542 2.42e-26 113
MS.gene067378.t1 MTR_1g026350 38.971 136 83 0 18 153 18 153 6.86e-25 101
MS.gene067378.t1 MTR_1g104810 25.333 525 304 17 23 472 57 568 6.99e-24 106
MS.gene067378.t1 MTR_3g087980 25.309 486 273 17 68 475 106 579 3.85e-23 104
MS.gene067378.t1 MTR_1g104810 25.751 466 272 14 68 472 49 501 8.40e-23 102
MS.gene067378.t1 MTR_1g030290 25.662 491 275 18 68 480 108 586 2.41e-22 101
MS.gene067378.t1 MTR_4g057755 55.556 72 32 0 411 482 46 117 5.65e-22 92.0
MS.gene067378.t1 MTR_1054s0020 25.978 358 238 11 200 549 432 770 8.42e-21 97.1
MS.gene067378.t1 MTR_2g098490 26.952 397 232 17 198 544 647 1035 2.39e-18 89.7
MS.gene067378.t1 MTR_7g109830 23.855 524 313 20 37 484 36 549 2.60e-18 89.0
MS.gene067378.t1 MTR_8g099420 25.564 399 217 15 140 484 180 552 3.95e-18 88.6
MS.gene067378.t1 MTR_4g066130 26.884 398 232 18 197 544 650 1038 9.23e-18 87.8
MS.gene067378.t1 MTR_8g083170 31.795 195 115 5 351 532 482 671 3.86e-16 82.4
MS.gene067378.t1 MTR_3g088890 51.562 64 31 0 457 520 10 73 3.05e-15 71.6
MS.gene067378.t1 MTR_3g084470 33.974 156 90 3 351 495 475 628 4.07e-14 75.9
MS.gene067378.t1 MTR_3g084450 31.792 173 102 5 335 495 256 424 9.26e-14 74.3
MS.gene067378.t1 MTR_4g013210 24.416 385 253 12 67 423 81 455 2.20e-13 72.8
MS.gene067378.t1 MTR_3g084450 31.792 173 102 5 335 495 464 632 2.21e-13 73.6
MS.gene067378.t1 MTR_1g086600 27.615 239 149 7 320 551 409 630 9.96e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067378.t1 AT4G19010 58.867 547 220 3 10 551 12 558 0.0 652
MS.gene067378.t1 AT1G20510 44.037 545 284 6 13 547 3 536 4.62e-160 468
MS.gene067378.t1 AT1G20480 41.982 555 299 6 14 555 21 565 7.53e-151 445
MS.gene067378.t1 AT1G20500 42.596 547 295 9 18 555 14 550 3.38e-144 427
MS.gene067378.t1 AT5G38120 41.007 556 292 10 12 547 3 542 2.27e-136 407
MS.gene067378.t1 AT4G05160 40.183 545 306 7 23 555 8 544 2.47e-136 407
MS.gene067378.t1 AT5G63380 40.255 549 304 7 14 547 11 550 1.27e-134 403
MS.gene067378.t1 AT1G20510 42.500 480 255 6 13 482 3 471 3.35e-132 394
MS.gene067378.t1 AT5G38120 39.962 528 281 10 12 519 3 514 1.14e-122 371
MS.gene067378.t1 AT1G20510 41.522 460 248 6 13 462 3 451 2.81e-121 365
MS.gene067378.t1 AT3G21240 41.558 539 288 12 30 555 30 554 1.16e-116 357
MS.gene067378.t1 AT1G51680 42.633 509 278 9 58 555 56 561 1.29e-111 344
MS.gene067378.t1 AT5G38120 39.715 491 260 10 12 482 3 477 7.67e-111 339
MS.gene067378.t1 AT5G38120 39.715 491 260 10 12 482 3 477 7.67e-111 339
MS.gene067378.t1 AT3G21230 39.583 528 301 10 40 553 43 566 7.70e-108 334
MS.gene067378.t1 AT1G65060 41.129 496 283 8 58 548 66 557 9.77e-108 334
MS.gene067378.t1 AT1G51680 42.283 473 259 9 58 519 56 525 1.21e-99 312
MS.gene067378.t1 AT1G62940 37.822 505 289 8 57 549 43 534 1.28e-97 307
MS.gene067378.t1 AT3G21230 39.271 494 282 10 40 519 43 532 2.86e-96 304
MS.gene067378.t1 AT3G21240 40.385 468 252 12 30 484 30 483 1.28e-91 290
MS.gene067378.t1 AT3G21240 40.385 468 252 12 30 484 30 483 1.28e-91 290
MS.gene067378.t1 AT1G20490 37.500 424 246 6 18 431 14 428 1.58e-89 283
MS.gene067378.t1 AT1G20490 37.500 424 246 6 18 431 14 428 1.58e-89 283
MS.gene067378.t1 AT1G51680 40.868 438 245 9 58 484 56 490 4.10e-86 275
MS.gene067378.t1 AT3G21230 38.512 457 263 10 40 482 43 495 2.73e-84 271
MS.gene067378.t1 AT1G65060 39.078 412 242 8 58 464 66 473 6.87e-76 249
MS.gene067378.t1 AT3G48990 29.652 489 320 12 67 545 30 504 2.35e-49 178
MS.gene067378.t1 AT3G21230 34.021 388 238 10 40 413 43 426 1.11e-48 175
MS.gene067378.t1 AT1G30520 28.060 531 299 18 77 549 42 547 9.28e-42 158
MS.gene067378.t1 AT1G30520 28.060 531 299 18 77 549 42 547 9.28e-42 158
MS.gene067378.t1 AT1G77240 29.198 524 312 21 67 552 43 545 1.28e-41 157
MS.gene067378.t1 AT1G21530 27.505 549 334 22 41 549 19 543 1.55e-41 157
MS.gene067378.t1 AT1G21540 26.847 555 340 20 41 552 19 550 3.00e-41 157
MS.gene067378.t1 AT1G21530 27.422 547 335 22 41 549 19 541 3.55e-40 154
MS.gene067378.t1 AT5G16370 27.921 505 311 19 80 549 53 539 4.55e-38 147
MS.gene067378.t1 AT1G65880 27.410 529 324 17 66 549 38 551 3.23e-37 145
MS.gene067378.t1 AT1G65890 26.880 532 322 18 66 549 38 550 5.64e-37 145
MS.gene067378.t1 AT5G16340 26.786 504 318 16 80 549 53 539 1.93e-35 140
MS.gene067378.t1 AT3G16910 26.882 558 329 22 41 548 27 555 1.36e-34 138
MS.gene067378.t1 AT3G16170 23.986 567 355 18 41 554 64 607 5.96e-34 136
MS.gene067378.t1 AT1G75960 30.914 372 222 13 196 548 183 538 7.31e-34 135
MS.gene067378.t1 AT1G66120 27.238 525 322 18 66 549 38 543 1.67e-33 135
MS.gene067378.t1 AT1G30520 27.333 450 255 16 77 482 42 463 1.35e-32 131
MS.gene067378.t1 AT1G30520 27.333 450 255 16 77 482 42 463 1.35e-32 131
MS.gene067378.t1 AT1G20560 28.543 508 304 18 80 549 54 540 1.60e-32 132
MS.gene067378.t1 AT1G76290 27.163 497 317 16 80 548 52 531 1.07e-31 129
MS.gene067378.t1 AT5G36880 25.472 530 347 15 67 555 197 719 6.28e-30 125
MS.gene067378.t1 AT5G36880 25.472 530 347 15 67 555 147 669 6.35e-30 125
MS.gene067378.t1 AT5G36880 25.472 530 347 15 67 555 147 669 6.35e-30 125
MS.gene067378.t1 AT5G36880 25.472 530 347 15 67 555 147 669 6.35e-30 125
MS.gene067378.t1 AT5G36880 25.472 530 347 15 67 555 147 669 6.35e-30 125
MS.gene067378.t1 AT1G20560 30.728 371 218 13 198 548 110 461 1.08e-29 122
MS.gene067378.t1 AT2G17650 27.520 367 242 11 196 549 240 595 5.88e-27 115
MS.gene067378.t1 AT5G27600 27.533 523 291 18 23 470 60 569 6.38e-26 112
MS.gene067378.t1 AT1G68270 26.702 382 210 14 193 548 182 519 7.68e-26 111
MS.gene067378.t1 AT5G36880 25.435 460 298 14 67 488 147 599 2.54e-23 104
MS.gene067378.t1 AT1G64400 24.952 517 293 18 21 456 21 523 5.53e-23 103
MS.gene067378.t1 AT1G77590 24.385 488 286 15 68 482 107 584 3.80e-21 98.2
MS.gene067378.t1 AT3G05970 25.573 524 294 17 26 470 63 569 6.35e-21 97.4
MS.gene067378.t1 AT1G49430 24.618 524 310 19 36 484 40 553 6.35e-21 97.4
MS.gene067378.t1 AT1G77590 27.027 370 198 14 175 482 79 438 4.58e-20 94.4
MS.gene067378.t1 AT4G11030 22.904 489 300 16 51 470 58 538 1.60e-18 89.7
MS.gene067378.t1 AT4G23850 23.392 513 304 21 22 456 22 523 2.10e-18 89.4
MS.gene067378.t1 AT3G23790 29.651 172 103 5 335 493 465 631 2.89e-12 70.1
MS.gene067378.t1 AT2G47240 30.952 168 110 5 320 484 326 490 3.95e-11 66.2
MS.gene067378.t1 AT2G47240 30.952 168 110 5 320 484 385 549 4.17e-11 66.2
MS.gene067378.t1 AT2G47240 30.952 168 110 5 320 484 385 549 4.17e-11 66.2
MS.gene067378.t1 AT2G47240 30.952 168 110 5 320 484 385 549 4.17e-11 66.2

Find 122 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGGATAGTAAATTGAATT+TGG 0.150999 5.2:+18228333 None:intergenic
GCCCTTATTGATACCTCTTC+TGG 0.159911 5.2:-18228459 MS.gene067378:CDS
TATCAAATACAAGGGCTTTC+AGG 0.229499 5.2:-18226891 MS.gene067378:intron
TTGACATTGACGAGACTATT+AGG 0.230432 5.2:-18227798 MS.gene067378:CDS
TATTGCTATGGTTGAGCTTT+TGG 0.241203 5.2:-18227965 MS.gene067378:CDS
TACTATCCCATTGTACTATT+AGG 0.254485 5.2:-18228321 MS.gene067378:CDS
TTGCCGATGTTCCATTTATA+TGG 0.273927 5.2:-18227883 MS.gene067378:CDS
ACCTGTATAAAATCAACATT+AGG 0.277352 5.2:+18227604 None:intergenic
ATAGTGGATGTCGCCGTTAC+AGG 0.280831 5.2:-18226705 MS.gene067378:CDS
AGAGCGATAAACCATATAAA+TGG 0.308944 5.2:+18227872 None:intergenic
ATACCATCACTCACCCTTCT+TGG 0.316236 5.2:-18228596 MS.gene067378:CDS
GCGAAGGCAAAGGGTGTAAA+TGG 0.324668 5.2:-18227712 MS.gene067378:CDS
TGCTACGGGATTATTGTCAT+TGG 0.327188 5.2:-18227848 MS.gene067378:CDS
TGGGATAGTAAATTGAATTT+GGG 0.341309 5.2:+18228334 None:intergenic
TTAGTCAAATCAATGGCTTC+TGG 0.343881 5.2:-18228405 MS.gene067378:CDS
CCCTTATTGATACCTCTTCT+GGG 0.345564 5.2:-18228458 MS.gene067378:CDS
AGCAGTTTCTGAGTTGCTTT+CGG 0.356180 5.2:+18226299 None:intergenic
GTCTCGTCAATGTCAAATTT+CGG 0.365775 5.2:+18227805 None:intergenic
ACGGCGACATCCACTATTTC+AGG 0.369334 5.2:+18226711 None:intergenic
TGCATGCCTTAGAGTTTAGA+AGG 0.372200 5.2:+18226274 None:intergenic
ATGACTGAGTCGGGTGCATT+AGG 0.374967 5.2:-18227454 MS.gene067378:CDS
CAGGCAGAAGTGGTGAGCTT+TGG 0.376467 5.2:-18227321 MS.gene067378:CDS
GGAGATTCCAGTGGCATTTG+TGG 0.385560 5.2:-18226568 MS.gene067378:CDS
AACGATACAACGTCGAGAAA+AGG 0.386540 5.2:+18228513 None:intergenic
TGCTTGCCTTCTAAACTCTA+AGG 0.391570 5.2:-18226280 None:intergenic
GTGTCTTCTTGTTTAATAAC+CGG 0.393788 5.2:+18228057 None:intergenic
ACTTTGCGAACCTTCTTGAA+TGG 0.396762 5.2:+18226367 None:intergenic
TGACATTGACGAGACTATTA+GGG 0.397999 5.2:-18227797 MS.gene067378:CDS
CTCCCCAACTTCTTCATCCT+TGG 0.399744 5.2:+18226587 None:intergenic
TCCCAGAAGAGGTATCAATA+AGG 0.401871 5.2:+18228457 None:intergenic
GTGTATACTATTGTAAATTC+CGG 0.412626 5.2:+18228560 None:intergenic
GCTACGGGATTATTGTCATT+GGG 0.419178 5.2:-18227847 MS.gene067378:CDS
GGCTTCTGGTCTTCATAAAA+CGG 0.424102 5.2:-18228391 MS.gene067378:CDS
TTTGAAGCAGGCTTGTTTCT+CGG 0.424271 5.2:-18228124 MS.gene067378:CDS
AAACAAAGTTACCAGAAATC+AGG 0.431755 5.2:+18228091 None:intergenic
GTTAAGAAATTAGAACCATT+AGG 0.435854 5.2:-18228183 MS.gene067378:CDS
TAACAACTTTGAGTAAGAAA+TGG 0.436153 5.2:+18228431 None:intergenic
TGGTTTATCGCTCTTTGCTA+CGG 0.436373 5.2:-18227863 MS.gene067378:CDS
TGGAGGCAAAAGTGGTAGAT+TGG 0.438591 5.2:-18227366 MS.gene067378:CDS
CCTGTATAAAATCAACATTA+GGG 0.441453 5.2:+18227605 None:intergenic
GTGCCAAGGATGAAGAAGTT+GGG 0.442033 5.2:-18226590 MS.gene067378:CDS
AAAGATGGTTGGCTACATAC+TGG 0.442873 5.2:-18226974 MS.gene067378:CDS
GCTGTCAATGCCGACAATAT+CGG 0.443469 5.2:+18227733 None:intergenic
GAAGCCATTGATTTGACTAA+AGG 0.445775 5.2:+18228408 None:intergenic
CAAAGCTCACCACTTCTGCC+TGG 0.447396 5.2:+18227322 None:intergenic
TATCGACTTCGTTGCTAAGC+AGG 0.451419 5.2:-18226508 MS.gene067378:intron
TGTTGTTCCACCGATATTGT+CGG 0.453280 5.2:-18227743 MS.gene067378:CDS
TTTCGCAACTATTCCTCACT+AGG 0.455586 5.2:-18227406 MS.gene067378:CDS
GTTACCTTTAGTCAAATCAA+TGG 0.458910 5.2:-18228412 MS.gene067378:CDS
AAAGCTCACCACTTCTGCCT+GGG 0.459009 5.2:+18227323 None:intergenic
ACGAAGTCGATAACATCCTT+TGG 0.459798 5.2:+18226519 None:intergenic
GCATTTGTGGTCAAGAAAGT+TGG 0.466162 5.2:-18226555 MS.gene067378:CDS
ACGATACAACGTCGAGAAAA+GGG 0.466770 5.2:+18228514 None:intergenic
TTCTGTGCCAGAAAATGAAA+AGG 0.468038 5.2:-18228148 MS.gene067378:CDS
AATCAACATTAGGGAAAGCT+TGG 0.471169 5.2:+18227614 None:intergenic
CCCAGAAGAGGTATCAATAA+GGG 0.474839 5.2:+18228458 None:intergenic
GGTTTATCGCTCTTTGCTAC+GGG 0.477009 5.2:-18227862 MS.gene067378:CDS
ATAACACCTAATAGTACAAT+GGG 0.478275 5.2:+18228315 None:intergenic
TTCTTGACCACAAATGCCAC+TGG 0.484108 5.2:+18226561 None:intergenic
TCATAGAAATCTTATTGCTA+TGG 0.487900 5.2:-18227977 MS.gene067378:CDS
GCTTCTGGTCTTCATAAAAC+GGG 0.488629 5.2:-18228390 MS.gene067378:CDS
CTTAGGATACTTGAATAATG+AGG 0.494593 5.2:-18227020 MS.gene067378:intron
ATTTCATCAACACTACTAAG+AGG 0.496577 5.2:+18228261 None:intergenic
GAAAGAATTTATCAAATACA+AGG 0.497893 5.2:-18226900 MS.gene067378:CDS
GGGTCGGTCGGAAGGCTCCT+AGG 0.498932 5.2:+18228534 None:intergenic
CACAGACCTTGGAATCTTGT+CGG 0.500104 5.2:+18226336 None:intergenic
GAAATAATAGGAATGCCTAA+TGG 0.507899 5.2:+18228168 None:intergenic
AGTGCCAAGGATGAAGAAGT+TGG 0.508872 5.2:-18226591 MS.gene067378:CDS
ATCTTCCCAGTCACAGACCT+TGG 0.509926 5.2:+18226325 None:intergenic
TAAGTTGCAGAGTTTGAGAC+AGG 0.511530 5.2:-18227686 MS.gene067378:CDS
AACACAGCTTCTAAATCAGC+AGG 0.514041 5.2:+18226744 None:intergenic
GTTTATGGAATGACTGAGTC+GGG 0.516171 5.2:-18227463 MS.gene067378:CDS
AAATGAAAAGGATTTGAAGC+AGG 0.517247 5.2:-18228136 MS.gene067378:CDS
AAAGTGGTAGATTGGAATAG+TGG 0.520566 5.2:-18227358 MS.gene067378:CDS
GGTTTATGGAATGACTGAGT+CGG 0.521041 5.2:-18227464 MS.gene067378:CDS
AGTGGTGAGCTTTGGTTGAG+AGG 0.521413 5.2:-18227313 MS.gene067378:CDS
AAGATTCCAAGGTCTGTGAC+TGG 0.524838 5.2:-18226331 MS.gene067378:CDS
TCCAAACATGGAGGCAAAAG+TGG 0.526229 5.2:-18227374 MS.gene067378:CDS
ATGTCGACAATTGATAAAGA+TGG 0.526979 5.2:-18226989 MS.gene067378:CDS
ACAATAGTATACACTCTCCT+AGG 0.538812 5.2:-18228551 MS.gene067378:CDS
TTCGCAACTATTCCTCACTA+GGG 0.544004 5.2:-18227405 MS.gene067378:CDS
GCAGGTTGCTCCATTCAAGA+AGG 0.544629 5.2:-18226377 MS.gene067378:intron
CGAGAAAAGGGTCGGTCGGA+AGG 0.547817 5.2:+18228526 None:intergenic
TAGTGGATGTCGCCGTTACA+GGG 0.551826 5.2:-18226704 MS.gene067378:intron
CATAACACCTAATAGTACAA+TGG 0.557647 5.2:+18228314 None:intergenic
TGGAAGTGTACTTTCTCCAA+AGG 0.559244 5.2:-18226535 MS.gene067378:CDS
GCATTGACAGCGAAGGCAAA+GGG 0.561700 5.2:-18227721 MS.gene067378:CDS
ACACTACTAAGAGGATTCAA+TGG 0.565007 5.2:+18228270 None:intergenic
GGCATTGACAGCGAAGGCAA+AGG 0.570107 5.2:-18227722 MS.gene067378:CDS
ATTTGATAAATTCTTTCAAG+CGG 0.572308 5.2:+18226906 None:intergenic
AGAGGTCCTTCAATTATGAA+AGG 0.572900 5.2:-18227295 MS.gene067378:intron
AAGCTCACCACTTCTGCCTG+GGG 0.573330 5.2:+18227324 None:intergenic
CGACAATTGATAAAGATGGT+TGG 0.573781 5.2:-18226985 MS.gene067378:CDS
AGATTCCAAGGTCTGTGACT+GGG 0.575854 5.2:-18226330 MS.gene067378:CDS
AAAGAATTTATCAAATACAA+GGG 0.580755 5.2:-18226899 MS.gene067378:CDS
TGCCAAGGATGAAGAAGTTG+GGG 0.582989 5.2:-18226589 MS.gene067378:CDS
CATAAAACGGGAGTTTCACA+AGG 0.587188 5.2:-18228378 MS.gene067378:CDS
CTATTAGGTGTTATGTACCT+AGG 0.587403 5.2:-18228306 MS.gene067378:CDS
AAACCATATAAATGGAACAT+CGG 0.588130 5.2:+18227880 None:intergenic
GGTACTACTGGTGTGAGCAA+AGG 0.591807 5.2:-18228012 MS.gene067378:CDS
ATTCAAGAAGGTTCGCAAAG+TGG 0.594840 5.2:-18226365 MS.gene067378:CDS
TTCATATACTTACCCTGTAA+CGG 0.602675 5.2:+18226692 None:intergenic
AGAAATGGAAGTCCCAGAAG+AGG 0.608722 5.2:+18228446 None:intergenic
AGTTTCTGAGTTGCTTTCGG+AGG 0.613327 5.2:+18226302 None:intergenic
TGGAGTTAACAACCCTAGTG+AGG 0.617548 5.2:+18227393 None:intergenic
TCGGGTGCATTAGGAGCATG+CGG 0.630001 5.2:-18227445 MS.gene067378:CDS
GTCAATGCCGACAATATCGG+TGG 0.636107 5.2:+18227736 None:intergenic
AGGGCTAAAACCCCATCACG+AGG 0.639322 5.2:+18228477 None:intergenic
AGCTCACCACTTCTGCCTGG+GGG 0.642781 5.2:+18227325 None:intergenic
AGGGTTGTTAACTCCAAACA+TGG 0.645259 5.2:-18227386 MS.gene067378:CDS
TCTTCACCGACAAGATTCCA+AGG 0.652098 5.2:-18226342 MS.gene067378:CDS
ATACCAAGAAGGGTGAGTGA+TGG 0.654569 5.2:+18228593 None:intergenic
ACGTCGAGAAAAGGGTCGGT+CGG 0.657613 5.2:+18228522 None:intergenic
ATTGTCGGCATTGACAGCGA+AGG 0.665271 5.2:-18227728 MS.gene067378:CDS
ACAGCTTCTAAATCAGCAGG+AGG 0.675381 5.2:+18226747 None:intergenic
TTGGGAGTAGAAGCAAAACA+AGG 0.677547 5.2:+18228352 None:intergenic
CGTAAGCGAGTTAATGACTG+TGG 0.681914 5.2:-18228237 MS.gene067378:CDS
TACAACGTCGAGAAAAGGGT+CGG 0.686772 5.2:+18228518 None:intergenic
ATGGTGTAACAACAGCACCT+AGG 0.700872 5.2:+18228289 None:intergenic
GGGCTAAAACCCCATCACGA+GGG 0.712324 5.2:+18228478 None:intergenic
AGAAGTTGGGGAGATTCCAG+TGG 0.713242 5.2:-18226577 MS.gene067378:CDS
GTTGTTAACTCCAAACATGG+AGG 0.748305 5.2:-18227383 MS.gene067378:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAAATTATTTAAATGA+TGG + chr5.2:18227382-18227401 None:intergenic 10.0%
!! AAAGAATTTATCAAATACAA+GGG - chr5.2:18228013-18228032 MS.gene067378:CDS 15.0%
!! GTTTGATTAATAATTTCAAT+TGG + chr5.2:18227858-18227877 None:intergenic 15.0%
!! TTATATTTAAATGTCTAAAG+AGG + chr5.2:18228271-18228290 None:intergenic 15.0%
!! ATTTGATAAATTCTTTCAAG+CGG + chr5.2:18228009-18228028 None:intergenic 20.0%
!! GAAAGAATTTATCAAATACA+AGG - chr5.2:18228012-18228031 MS.gene067378:CDS 20.0%
!!! AACTTTTTCCTTAATTTCTT+AGG - chr5.2:18227875-18227894 MS.gene067378:CDS 20.0%
!!! AATTTTAACCCTTTTTTTCT+TGG - chr5.2:18228513-18228532 MS.gene067378:CDS 20.0%
!!! ATTGTTTATTTTGATCAAGA+TGG - chr5.2:18227965-18227984 MS.gene067378:CDS 20.0%
!!! ATTGTTTCTTTTTAGGTTTA+TGG - chr5.2:18227434-18227453 MS.gene067378:CDS 20.0%
!!! CTGTAAAATTGTTTCTTTTT+AGG - chr5.2:18227427-18227446 MS.gene067378:CDS 20.0%
!!! TCTAATTTCTTAACATTTTC+AGG + chr5.2:18226723-18226742 None:intergenic 20.0%
!!! TTTTTACCTTTCATAATTGA+AGG + chr5.2:18227626-18227645 None:intergenic 20.0%
! AAACCATATAAATGGAACAT+CGG + chr5.2:18227035-18227054 None:intergenic 25.0%
! ACCTGTATAAAATCAACATT+AGG + chr5.2:18227311-18227330 None:intergenic 25.0%
! ACTTGAATCAATGTTAGTAT+TGG - chr5.2:18228479-18228498 MS.gene067378:CDS 25.0%
! ATAACACCTAATAGTACAAT+GGG + chr5.2:18226600-18226619 None:intergenic 25.0%
! ATGGGATAGTAAATTGAATT+TGG + chr5.2:18226582-18226601 None:intergenic 25.0%
! CCTGTATAAAATCAACATTA+GGG + chr5.2:18227310-18227329 None:intergenic 25.0%
! GTGTATACTATTGTAAATTC+CGG + chr5.2:18226355-18226374 None:intergenic 25.0%
! GTTAAGAAATTAGAACCATT+AGG - chr5.2:18226729-18226748 MS.gene067378:CDS 25.0%
! TAAATACTAAAGGGATAACA+TGG - chr5.2:18227679-18227698 MS.gene067378:CDS 25.0%
! TAACAACTTTGAGTAAGAAA+TGG + chr5.2:18226484-18226503 None:intergenic 25.0%
! TCAAGTATCCTAAGAAATTA+AGG + chr5.2:18227886-18227905 None:intergenic 25.0%
! TCATAGAAATCTTATTGCTA+TGG - chr5.2:18226935-18226954 MS.gene067378:CDS 25.0%
! TGGGATAGTAAATTGAATTT+GGG + chr5.2:18226581-18226600 None:intergenic 25.0%
!! AATCAATGTTAGTATTGGAA+TGG - chr5.2:18228484-18228503 MS.gene067378:CDS 25.0%
!! TTCAAATCCTTTTCATTTTC+TGG + chr5.2:18226774-18226793 None:intergenic 25.0%
!! TTTATAGCTTTTGTGCTTAA+AGG + chr5.2:18227269-18227288 None:intergenic 25.0%
!!! GATTTTTTTAACCTGATTTC+TGG - chr5.2:18226810-18226829 MS.gene067378:intron 25.0%
!!! TTGTTTTCCTAGATTTGTTA+AGG - chr5.2:18227396-18227415 MS.gene067378:CDS 25.0%
AAACAAAGTTACCAGAAATC+AGG + chr5.2:18226824-18226843 None:intergenic 30.0%
AAGACAGAAACAAAAGTTAC+AGG + chr5.2:18227790-18227809 None:intergenic 30.0%
AATCAATCTCATGCAAATGT+AGG + chr5.2:18227741-18227760 None:intergenic 30.0%
AGAGCGATAAACCATATAAA+TGG + chr5.2:18227043-18227062 None:intergenic 30.0%
ATGTCGACAATTGATAAAGA+TGG - chr5.2:18227923-18227942 MS.gene067378:CDS 30.0%
ATTTCATCAACACTACTAAG+AGG + chr5.2:18226654-18226673 None:intergenic 30.0%
CATAACACCTAATAGTACAA+TGG + chr5.2:18226601-18226620 None:intergenic 30.0%
CTTAGGATACTTGAATAATG+AGG - chr5.2:18227892-18227911 MS.gene067378:CDS 30.0%
GAAATAATAGGAATGCCTAA+TGG + chr5.2:18226747-18226766 None:intergenic 30.0%
GGAAAATGATGAAACACTTA+AGG + chr5.2:18228294-18228313 None:intergenic 30.0%
GGGATAACATGGTAATATAA+AGG - chr5.2:18227690-18227709 MS.gene067378:CDS 30.0%
GTTACCTTTAGTCAAATCAA+TGG - chr5.2:18226500-18226519 MS.gene067378:intron 30.0%
TACTATCCCATTGTACTATT+AGG - chr5.2:18226591-18226610 MS.gene067378:CDS 30.0%
TTCATATACTTACCCTGTAA+CGG + chr5.2:18228223-18228242 None:intergenic 30.0%
! AAATGAAAAGGATTTGAAGC+AGG - chr5.2:18226776-18226795 MS.gene067378:intron 30.0%
! AACAGCTAGATACACATTTT+TGG + chr5.2:18227009-18227028 None:intergenic 30.0%
! AATTTTGCATCCTGAAATAG+TGG - chr5.2:18228191-18228210 MS.gene067378:CDS 30.0%
! CTATTGTAAATTCCGGTTTT+TGG + chr5.2:18226348-18226367 None:intergenic 30.0%
! GTGTCTTCTTGTTTAATAAC+CGG + chr5.2:18226858-18226877 None:intergenic 30.0%
!! TTTTCTGGCACAGAAATAAT+AGG + chr5.2:18226759-18226778 None:intergenic 30.0%
!! TTTTTGGTGAATACCAAGAA+GGG + chr5.2:18226332-18226351 None:intergenic 30.0%
!!! CCCTAATGTTGATTTTATAC+AGG - chr5.2:18227307-18227326 MS.gene067378:CDS 30.0%
AAAGTGGTAGATTGGAATAG+TGG - chr5.2:18227554-18227573 MS.gene067378:intron 35.0%
AATCAACATTAGGGAAAGCT+TGG + chr5.2:18227301-18227320 None:intergenic 35.0%
AATTGGAATAGCAACAAGAC+AGG + chr5.2:18227841-18227860 None:intergenic 35.0%
ACAATAGTATACACTCTCCT+AGG - chr5.2:18226361-18226380 MS.gene067378:CDS 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr5.2:18226645-18226664 None:intergenic 35.0%
AGAGGTCCTTCAATTATGAA+AGG - chr5.2:18227617-18227636 MS.gene067378:CDS 35.0%
CGACAATTGATAAAGATGGT+TGG - chr5.2:18227927-18227946 MS.gene067378:CDS 35.0%
GTCTCGTCAATGTCAAATTT+CGG + chr5.2:18227110-18227129 None:intergenic 35.0%
TATCAAATACAAGGGCTTTC+AGG - chr5.2:18228021-18228040 MS.gene067378:CDS 35.0%
TCTTGGTATTCACCAAAAAC+CGG - chr5.2:18226333-18226352 MS.gene067378:CDS 35.0%
TGACATTGACGAGACTATTA+GGG - chr5.2:18227115-18227134 MS.gene067378:intron 35.0%
TGCACATCCTTAACAAATCT+AGG + chr5.2:18227406-18227425 None:intergenic 35.0%
TTAGTCAAATCAATGGCTTC+TGG - chr5.2:18226507-18226526 MS.gene067378:intron 35.0%
TTCTGTGCCAGAAAATGAAA+AGG - chr5.2:18226764-18226783 MS.gene067378:intron 35.0%
TTGACATTGACGAGACTATT+AGG - chr5.2:18227114-18227133 MS.gene067378:intron 35.0%
TTGCCGATGTTCCATTTATA+TGG - chr5.2:18227029-18227048 MS.gene067378:intron 35.0%
! CTATTAGGTGTTATGTACCT+AGG - chr5.2:18226606-18226625 MS.gene067378:intron 35.0%
! GCTGCTATATTGTTTTCTTC+TGG - chr5.2:18226879-18226898 MS.gene067378:intron 35.0%
! TATTGCTATGGTTGAGCTTT+TGG - chr5.2:18226947-18226966 MS.gene067378:CDS 35.0%
! TTGTTTTCTTCTGGTACTAC+TGG - chr5.2:18226888-18226907 MS.gene067378:intron 35.0%
! TTTTCCATATATCAGTGCCA+AGG - chr5.2:18228308-18228327 MS.gene067378:CDS 35.0%
!! GAAGCCATTGATTTGACTAA+AGG + chr5.2:18226507-18226526 None:intergenic 35.0%
!! TGTTTAATAACCGGCTTTTG+AGG + chr5.2:18226849-18226868 None:intergenic 35.0%
!!! GTTTTTGGTGAATACCAAGA+AGG + chr5.2:18226333-18226352 None:intergenic 35.0%
!!! TTAACCCTTTTTTTCTTGGC+AGG - chr5.2:18228517-18228536 MS.gene067378:CDS 35.0%
AACACAGCTTCTAAATCAGC+AGG + chr5.2:18228171-18228190 None:intergenic 40.0%
AACGATACAACGTCGAGAAA+AGG + chr5.2:18226402-18226421 None:intergenic 40.0%
ACGAAGTCGATAACATCCTT+TGG + chr5.2:18228396-18228415 None:intergenic 40.0%
ACGATACAACGTCGAGAAAA+GGG + chr5.2:18226401-18226420 None:intergenic 40.0%
ACTTTGCGAACCTTCTTGAA+TGG + chr5.2:18228548-18228567 None:intergenic 40.0%
AGCAACCTGCCAAGAAAAAA+AGG + chr5.2:18228525-18228544 None:intergenic 40.0%
AGGGTTGTTAACTCCAAACA+TGG - chr5.2:18227526-18227545 MS.gene067378:intron 40.0%
ATTCAAGAAGGTTCGCAAAG+TGG - chr5.2:18228547-18228566 MS.gene067378:CDS 40.0%
CATAAAACGGGAGTTTCACA+AGG - chr5.2:18226534-18226553 MS.gene067378:CDS 40.0%
CCCTTATTGATACCTCTTCT+GGG - chr5.2:18226454-18226473 MS.gene067378:intron 40.0%
GCAACCTGCCAAGAAAAAAA+GGG + chr5.2:18228524-18228543 None:intergenic 40.0%
GCATTTGTGGTCAAGAAAGT+TGG - chr5.2:18228357-18228376 MS.gene067378:CDS 40.0%
GCTACGGGATTATTGTCATT+GGG - chr5.2:18227065-18227084 MS.gene067378:intron 40.0%
GGTTTATGGAATGACTGAGT+CGG - chr5.2:18227448-18227467 MS.gene067378:CDS 40.0%
GTTGTTAACTCCAAACATGG+AGG - chr5.2:18227529-18227548 MS.gene067378:intron 40.0%
GTTTATGGAATGACTGAGTC+GGG - chr5.2:18227449-18227468 MS.gene067378:CDS 40.0%
TGCTACGGGATTATTGTCAT+TGG - chr5.2:18227064-18227083 MS.gene067378:intron 40.0%
TGGTTTATCGCTCTTTGCTA+CGG - chr5.2:18227049-18227068 MS.gene067378:intron 40.0%
TGTTGTTCCACCGATATTGT+CGG - chr5.2:18227169-18227188 MS.gene067378:intron 40.0%
TTCGCAACTATTCCTCACTA+GGG - chr5.2:18227507-18227526 MS.gene067378:intron 40.0%
TTGGGAGTAGAAGCAAAACA+AGG + chr5.2:18226563-18226582 None:intergenic 40.0%
TTTCGCAACTATTCCTCACT+AGG - chr5.2:18227506-18227525 MS.gene067378:intron 40.0%
! AAAGATGGTTGGCTACATAC+TGG - chr5.2:18227938-18227957 MS.gene067378:CDS 40.0%
! AGCAGTTTCTGAGTTGCTTT+CGG + chr5.2:18228616-18228635 None:intergenic 40.0%
! GCTTCTGGTCTTCATAAAAC+GGG - chr5.2:18226522-18226541 MS.gene067378:CDS 40.0%
! GGCTTCTGGTCTTCATAAAA+CGG - chr5.2:18226521-18226540 MS.gene067378:CDS 40.0%
! TAAGTTGCAGAGTTTGAGAC+AGG - chr5.2:18227226-18227245 MS.gene067378:intron 40.0%
! TGGAAGTGTACTTTCTCCAA+AGG - chr5.2:18228377-18228396 MS.gene067378:CDS 40.0%
! TTTGAAGCAGGCTTGTTTCT+CGG - chr5.2:18226788-18226807 MS.gene067378:intron 40.0%
!! CCCAGAAGAGGTATCAATAA+GGG + chr5.2:18226457-18226476 None:intergenic 40.0%
!! TCATCCTTGGCACTGATATA+TGG + chr5.2:18228315-18228334 None:intergenic 40.0%
!! TCCCAGAAGAGGTATCAATA+AGG + chr5.2:18226458-18226477 None:intergenic 40.0%
!!! ATTTTTTCTCACCCTCGTGA+TGG - chr5.2:18226423-18226442 MS.gene067378:intron 40.0%
!!! CTTTGTTTTGCCTCAAAAGC+CGG - chr5.2:18226836-18226855 MS.gene067378:intron 40.0%
!!! TTTTTTCTCACCCTCGTGAT+GGG - chr5.2:18226424-18226443 MS.gene067378:intron 40.0%
AAGATTCCAAGGTCTGTGAC+TGG - chr5.2:18228581-18228600 MS.gene067378:CDS 45.0%
ACAGCTTCTAAATCAGCAGG+AGG + chr5.2:18228168-18228187 None:intergenic 45.0%
ACAGGCAGAAAAGGTTCAAG+TGG + chr5.2:18227823-18227842 None:intergenic 45.0%
AGAAATGGAAGTCCCAGAAG+AGG + chr5.2:18226469-18226488 None:intergenic 45.0%
AGATTCCAAGGTCTGTGACT+GGG - chr5.2:18228582-18228601 MS.gene067378:CDS 45.0%
AGCAACAAGACAGGCAGAAA+AGG + chr5.2:18227832-18227851 None:intergenic 45.0%
AGTGCCAAGGATGAAGAAGT+TGG - chr5.2:18228321-18228340 MS.gene067378:CDS 45.0%
ATACCAAGAAGGGTGAGTGA+TGG + chr5.2:18226322-18226341 None:intergenic 45.0%
ATACCATCACTCACCCTTCT+TGG - chr5.2:18226316-18226335 MS.gene067378:CDS 45.0%
ATGGTGTAACAACAGCACCT+AGG + chr5.2:18226626-18226645 None:intergenic 45.0%
CACAGACCTTGGAATCTTGT+CGG + chr5.2:18228579-18228598 None:intergenic 45.0%
CGTAAGCGAGTTAATGACTG+TGG - chr5.2:18226675-18226694 MS.gene067378:intron 45.0%
GAAAAGGTTCAAGTGGCTTG+AGG + chr5.2:18227816-18227835 None:intergenic 45.0%
GCCCTTATTGATACCTCTTC+TGG - chr5.2:18226453-18226472 MS.gene067378:intron 45.0%
GCTGTCAATGCCGACAATAT+CGG + chr5.2:18227182-18227201 None:intergenic 45.0%
GGTTTATCGCTCTTTGCTAC+GGG - chr5.2:18227050-18227069 MS.gene067378:intron 45.0%
GTGCCAAGGATGAAGAAGTT+GGG - chr5.2:18228322-18228341 MS.gene067378:CDS 45.0%
TACAACGTCGAGAAAAGGGT+CGG + chr5.2:18226397-18226416 None:intergenic 45.0%
TCCAAACATGGAGGCAAAAG+TGG - chr5.2:18227538-18227557 MS.gene067378:intron 45.0%
TCTTCACCGACAAGATTCCA+AGG - chr5.2:18228570-18228589 MS.gene067378:CDS 45.0%
TGCCAAGGATGAAGAAGTTG+GGG - chr5.2:18228323-18228342 MS.gene067378:CDS 45.0%
TGGAGGCAAAAGTGGTAGAT+TGG - chr5.2:18227546-18227565 MS.gene067378:intron 45.0%
TGGAGTTAACAACCCTAGTG+AGG + chr5.2:18227522-18227541 None:intergenic 45.0%
TTCTTGACCACAAATGCCAC+TGG + chr5.2:18228354-18228373 None:intergenic 45.0%
! AGTTTCTGAGTTGCTTTCGG+AGG + chr5.2:18228613-18228632 None:intergenic 45.0%
! GGGTGAGTGATGGTATTTTG+TGG + chr5.2:18226312-18226331 None:intergenic 45.0%
! TTTTTCTCACCCTCGTGATG+GGG - chr5.2:18226425-18226444 MS.gene067378:intron 45.0%
!! ACCACTTTTGCCTCCATGTT+TGG + chr5.2:18227542-18227561 None:intergenic 45.0%
!! TATCGACTTCGTTGCTAAGC+AGG - chr5.2:18228404-18228423 MS.gene067378:CDS 45.0%
!! ATAATAAATATAAATACTAA+AGG - chr5.2:18227669-18227688 MS.gene067378:CDS 5.0%
!! TAATAAATATAAATACTAAA+GGG - chr5.2:18227670-18227689 MS.gene067378:CDS 5.0%
AAAGCTCACCACTTCTGCCT+GGG + chr5.2:18227592-18227611 None:intergenic 50.0%
ACGGCGACATCCACTATTTC+AGG + chr5.2:18228204-18228223 None:intergenic 50.0%
AGAAGTTGGGGAGATTCCAG+TGG - chr5.2:18228335-18228354 MS.gene067378:CDS 50.0%
ATCTTCCCAGTCACAGACCT+TGG + chr5.2:18228590-18228609 None:intergenic 50.0%
ATGACTGAGTCGGGTGCATT+AGG - chr5.2:18227458-18227477 MS.gene067378:CDS 50.0%
CTCCCCAACTTCTTCATCCT+TGG + chr5.2:18228328-18228347 None:intergenic 50.0%
GCAGGTTGCTCCATTCAAGA+AGG - chr5.2:18228535-18228554 MS.gene067378:CDS 50.0%
GGAGATTCCAGTGGCATTTG+TGG - chr5.2:18228344-18228363 MS.gene067378:CDS 50.0%
GGTACTACTGGTGTGAGCAA+AGG - chr5.2:18226900-18226919 MS.gene067378:CDS 50.0%
GTCAATGCCGACAATATCGG+TGG + chr5.2:18227179-18227198 None:intergenic 50.0%
! AGTGGTGAGCTTTGGTTGAG+AGG - chr5.2:18227599-18227618 MS.gene067378:intron 50.0%
! CTATGGTTGAGCTTTTGGCG+AGG - chr5.2:18226952-18226971 MS.gene067378:CDS 50.0%
! GCATTGACAGCGAAGGCAAA+GGG - chr5.2:18227191-18227210 MS.gene067378:intron 50.0%
! GCGAAGGCAAAGGGTGTAAA+TGG - chr5.2:18227200-18227219 MS.gene067378:intron 50.0%
! TAGTGGATGTCGCCGTTACA+GGG - chr5.2:18228208-18228227 MS.gene067378:CDS 50.0%
!! ATAGTGGATGTCGCCGTTAC+AGG - chr5.2:18228207-18228226 MS.gene067378:CDS 50.0%
!! ATTGTCGGCATTGACAGCGA+AGG - chr5.2:18227184-18227203 MS.gene067378:intron 50.0%
AAGCTCACCACTTCTGCCTG+GGG + chr5.2:18227591-18227610 None:intergenic 55.0%
ACGTCGAGAAAAGGGTCGGT+CGG + chr5.2:18226393-18226412 None:intergenic 55.0%
AGGGCTAAAACCCCATCACG+AGG + chr5.2:18226438-18226457 None:intergenic 55.0%
CAAAGCTCACCACTTCTGCC+TGG + chr5.2:18227593-18227612 None:intergenic 55.0%
CAGGCAGAAGTGGTGAGCTT+TGG - chr5.2:18227591-18227610 MS.gene067378:intron 55.0%
GGGCTAAAACCCCATCACGA+GGG + chr5.2:18226437-18226456 None:intergenic 55.0%
! TCGGGTGCATTAGGAGCATG+CGG - chr5.2:18227467-18227486 MS.gene067378:intron 55.0%
! TTTTTGCCCCCAGGCAGAAG+TGG - chr5.2:18227581-18227600 MS.gene067378:intron 55.0%
!! GGCATTGACAGCGAAGGCAA+AGG - chr5.2:18227190-18227209 MS.gene067378:intron 55.0%
!!! AGTGGTGCTTTTTTGCCCCC+AGG - chr5.2:18227572-18227591 MS.gene067378:intron 55.0%
AGCTCACCACTTCTGCCTGG+GGG + chr5.2:18227590-18227609 None:intergenic 60.0%
CGAGAAAAGGGTCGGTCGGA+AGG + chr5.2:18226389-18226408 None:intergenic 60.0%
GGGTCGGTCGGAAGGCTCCT+AGG + chr5.2:18226381-18226400 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 18226282 18228652 18226282 ID=MS.gene067378
chr5.2 mRNA 18226282 18228652 18226282 ID=MS.gene067378.t1;Parent=MS.gene067378
chr5.2 exon 18227606 18228652 18227606 ID=MS.gene067378.t1.exon1;Parent=MS.gene067378.t1
chr5.2 CDS 18227606 18228652 18227606 ID=cds.MS.gene067378.t1;Parent=MS.gene067378.t1
chr5.2 exon 18227296 18227485 18227296 ID=MS.gene067378.t1.exon2;Parent=MS.gene067378.t1
chr5.2 CDS 18227296 18227485 18227296 ID=cds.MS.gene067378.t1;Parent=MS.gene067378.t1
chr5.2 exon 18226892 18227037 18226892 ID=MS.gene067378.t1.exon3;Parent=MS.gene067378.t1
chr5.2 CDS 18226892 18227037 18226892 ID=cds.MS.gene067378.t1;Parent=MS.gene067378.t1
chr5.2 exon 18226705 18226772 18226705 ID=MS.gene067378.t1.exon4;Parent=MS.gene067378.t1
chr5.2 CDS 18226705 18226772 18226705 ID=cds.MS.gene067378.t1;Parent=MS.gene067378.t1
chr5.2 exon 18226509 18226611 18226509 ID=MS.gene067378.t1.exon5;Parent=MS.gene067378.t1
chr5.2 CDS 18226509 18226611 18226509 ID=cds.MS.gene067378.t1;Parent=MS.gene067378.t1
chr5.2 exon 18226282 18226395 18226282 ID=MS.gene067378.t1.exon6;Parent=MS.gene067378.t1
chr5.2 CDS 18226282 18226395 18226282 ID=cds.MS.gene067378.t1;Parent=MS.gene067378.t1
Gene Sequence

>MS.gene067378

ATGGCTGCAAATTTAAACTATGACATGCCACAAAATACCATCACTCACCCTTCTTGGTATTCACCAAAAACCGGAATTTACAATAGTATACACTCTCCTAGGAGCCTTCCGACCGACCCTTTTCTCGACGTTGTATCGTTCATTTTTTCTCACCCTCGTGATGGGGTTTTAGCCCTTATTGATACCTCTTCTGGGACTTCCATTTCTTACTCAAAGTTGTTACCTTTAGTCAAATCAATGGCTTCTGGTCTTCATAAAACGGGAGTTTCACAAGGTGACCTTGTTTTGCTTCTACTCCCAAATTCAATTTACTATCCCATTGTACTATTAGGTGTTATGTACCTAGGTGCTGTTGTTACACCATTGAATCCTCTTAGTAGTGTTGATGAAATTCGTAAGCGAGTTAATGACTGTGGTGTGAGTTTTGCTTTTACTATACCTGAAAATGTTAAGAAATTAGAACCATTAGGCATTCCTATTATTTCTGTGCCAGAAAATGAAAAGGATTTGAAGCAGGCTTGTTTCTCGGATTTTTTTAACCTGATTTCTGGTAACTTTGTTTTGCCTCAAAAGCCGGTTATTAAACAAGAAGACACTGCTGCTATATTGTTTTCTTCTGGTACTACTGGTGTGAGCAAAGGAGTTGTTTTAACTCATAGAAATCTTATTGCTATGGTTGAGCTTTTGGCGAGGTTTGAAACTTCTAAATATGAATATTCTAGTTCCAAAAATGTGTATCTAGCTGTTTTGCCGATGTTCCATTTATATGGTTTATCGCTCTTTGCTACGGGATTATTGTCATTGGGTTCTACAATCGTTGTAATGCCGAAATTTGACATTGACGAGACTATTAGGGTGATCGATAAATATAATGTTACACACTTTCATGTTGTTCCACCGATATTGTCGGCATTGACAGCGAAGGCAAAGGGTGTAAATGGAAGTAAGTTGCAGAGTTTGAGACAGGTCAGTTCTAGTGCAGCGCCTTTAAGCACAAAAGCTATAAATGATTTTGTCCAAGCTTTCCCTAATGTTGATTTTATACAGGTAAAAATCTCAGAATAAAATAGTCTTACTTAAAGAATGCAAGTCATGATCCATCATTTAAATAATTTTGTTTTCCTAGATTTGTTAAGGATGTGCAGCTGTAAAATTGTTTCTTTTTAGGTTTATGGAATGACTGAGTCGGGTGCATTAGGAGCATGCGGTTTCAATACAGAAAAATTTCGCAACTATTCCTCACTAGGGTTGTTAACTCCAAACATGGAGGCAAAAGTGGTAGATTGGAATAGTGGTGCTTTTTTGCCCCCAGGCAGAAGTGGTGAGCTTTGGTTGAGAGGTCCTTCAATTATGAAAGGTAAAAATAATCTGCATTTTTTATGAATAGATAATAAATATAAATACTAAAGGGATAACATGGTAATATAAAGGTTTTGTCTATTGTGTGCAATCTTAACCTACATTTGCATGAGATTGATTCAATGATTTCAACTGTTTTGTTTGTTCCTGTAACTTTTGTTTCTGTCTTATTCCTCAAGCCACTTGAACCTTTTCTGCCTGTCTTGTTGCTATTCCAATTGAAATTATTAATCAAACTTTTTCCTTAATTTCTTAGGATACTTGAATAATGAGGAAGCTACAATGTCGACAATTGATAAAGATGGTTGGCTACATACTGGAGATATTGTTTATTTTGATCAAGATGGATATTTATATTTATTTGACCGCTTGAAAGAATTTATCAAATACAAGGGCTTTCAGGTATCAATTTTTTTCTTTATTTAAATTTTATAGTGATGTTACATATTTTTATTTTACAGTTTATTTTTACTCAATTAGTATGCATTGTTCTTATTTATATCACACATGATCTGTTACAGATTCCTCCTGCTGATTTAGAAGCTGTGTTAATTTTGCATCCTGAAATAGTGGATGTCGCCGTTACAGGGTAAGTATATGAACACTTCATTTATGTCTTCTAAGATGCCTCTTTAGACATTTAAATATAACCTTAAGTGTTTCATCATTTTCCATATATCAGTGCCAAGGATGAAGAAGTTGGGGAGATTCCAGTGGCATTTGTGGTCAAGAAAGTTGGAAGTGTACTTTCTCCAAAGGATGTTATCGACTTCGTTGCTAAGCAGGTTCTTCTTTATAAGACAGACATGAGTTCTATGATATTTGAATTAATATTACTACTTGAATCAATGTTAGTATTGGAATGGTTTTCAAATTTTAACCCTTTTTTTCTTGGCAGGTTGCTCCATTCAAGAAGGTTCGCAAAGTGGTCTTCACCGACAAGATTCCAAGGTCTGTGACTGGGAAGATCCTCCGAAAGCAACTCAGAAACTGCTTGCCTTCTAAACTCTAA
Protein sequence

>MS.gene067378.t1

MAANLNYDMPQNTITHPSWYSPKTGIYNSIHSPRSLPTDPFLDVVSFIFSHPRDGVLALIDTSSGTSISYSKLLPLVKSMASGLHKTGVSQGDLVLLLLPNSIYYPIVLLGVMYLGAVVTPLNPLSSVDEIRKRVNDCGVSFAFTIPENVKKLEPLGIPIISVPENEKDLKQACFSDFFNLISGNFVLPQKPVIKQEDTAAILFSSGTTGVSKGVVLTHRNLIAMVELLARFETSKYEYSSSKNVYLAVLPMFHLYGLSLFATGLLSLGSTIVVMPKFDIDETIRVIDKYNVTHFHVVPPILSALTAKAKGVNGSKLQSLRQVSSSAAPLSTKAINDFVQAFPNVDFIQVYGMTESGALGACGFNTEKFRNYSSLGLLTPNMEAKVVDWNSGAFLPPGRSGELWLRGPSIMKGYLNNEEATMSTIDKDGWLHTGDIVYFDQDGYLYLFDRLKEFIKYKGFQIPPADLEAVLILHPEIVDVAVTGAKDEEVGEIPVAFVVKKVGSVLSPKDVIDFVAKQVAPFKKVRKVVFTDKIPRSVTGKILRKQLRNCLPSKL