AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070122

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070122.t1 MTR_5g027500 95.070 568 28 0 1 568 1 568 0.0 1096
MS.gene070122.t1 MTR_5g027480 80.810 568 96 2 1 568 1 555 0.0 926
MS.gene070122.t1 MTR_4g077900 80.612 490 81 2 81 568 1 478 0.0 800
MS.gene070122.t1 MTR_1g026310 43.013 551 301 5 21 568 20 560 1.47e-152 449
MS.gene070122.t1 MTR_8g039720 43.659 552 298 7 21 568 17 559 2.72e-150 444
MS.gene070122.t1 MTR_1g090737 45.126 554 268 11 21 560 27 558 2.04e-142 424
MS.gene070122.t1 MTR_4g057730 41.513 542 293 8 26 560 10 534 4.95e-137 409
MS.gene070122.t1 MTR_2g095510 39.344 549 308 8 26 568 10 539 1.79e-131 395
MS.gene070122.t1 MTR_3g088880 41.406 512 267 9 55 560 64 548 7.31e-130 391
MS.gene070122.t1 MTR_1g063120 39.613 568 309 11 19 568 4 555 2.31e-129 390
MS.gene070122.t1 MTR_3g088870 41.326 513 274 8 59 566 67 557 1.45e-127 385
MS.gene070122.t1 MTR_1g063110 39.020 551 313 7 19 560 4 540 1.45e-125 380
MS.gene070122.t1 MTR_1g073180 39.245 530 281 9 43 562 30 528 3.94e-113 347
MS.gene070122.t1 MTR_4g057730 41.090 477 257 8 26 495 10 469 3.30e-112 343
MS.gene070122.t1 MTR_2g105570 38.959 557 312 12 22 566 7 547 6.25e-110 340
MS.gene070122.t1 MTR_4g128337 35.702 563 328 12 18 568 8 548 6.81e-103 321
MS.gene070122.t1 MTR_1g073180 37.965 511 276 9 43 543 52 531 1.17e-101 318
MS.gene070122.t1 MTR_4g057730 39.520 458 253 8 26 476 10 450 6.44e-101 313
MS.gene070122.t1 MTR_4g005750 37.868 544 302 12 34 566 22 540 2.42e-100 315
MS.gene070122.t1 MTR_3g460770 34.291 557 342 10 17 568 4 541 6.37e-99 311
MS.gene070122.t1 MTR_5g007640 36.462 554 319 10 25 566 53 585 1.07e-98 311
MS.gene070122.t1 MTR_2g095510 37.174 460 264 8 26 479 10 450 1.10e-95 300
MS.gene070122.t1 MTR_1g026430 36.990 392 234 5 21 409 20 401 1.73e-77 251
MS.gene070122.t1 MTR_1g026470 36.990 392 234 5 21 409 20 401 3.86e-77 250
MS.gene070122.t1 MTR_3g035130 29.051 506 324 13 63 558 25 505 1.76e-50 182
MS.gene070122.t1 MTR_1569s0010 26.806 526 335 12 69 562 22 529 2.30e-44 165
MS.gene070122.t1 MTR_5g076240 27.698 556 349 20 36 562 11 542 1.65e-43 163
MS.gene070122.t1 MTR_3g035130 28.478 460 294 13 63 512 25 459 9.09e-39 148
MS.gene070122.t1 MTR_2g044180 27.505 509 327 14 81 561 58 552 9.94e-38 147
MS.gene070122.t1 MTR_3g064420 25.933 536 332 15 66 561 37 547 3.25e-37 145
MS.gene070122.t1 MTR_2g044180 29.843 382 241 10 197 561 90 461 5.73e-37 143
MS.gene070122.t1 MTR_8g074040 27.810 525 316 19 81 568 94 592 1.68e-36 144
MS.gene070122.t1 MTR_0803s0020 27.853 517 295 14 71 554 263 734 2.74e-36 145
MS.gene070122.t1 MTR_4g011790 27.182 401 259 10 191 567 207 598 1.06e-35 141
MS.gene070122.t1 MTR_4g013210 28.278 389 236 11 210 567 69 445 1.09e-35 139
MS.gene070122.t1 MTR_5g008800 29.563 504 312 16 81 562 52 534 1.13e-35 141
MS.gene070122.t1 MTR_4g121600 26.990 515 328 16 78 561 55 552 2.08e-35 140
MS.gene070122.t1 MTR_4g011790 27.506 389 249 10 191 555 207 586 3.32e-34 137
MS.gene070122.t1 MTR_4g011790 26.829 410 258 12 191 567 207 607 6.63e-34 136
MS.gene070122.t1 MTR_5g076220 29.076 368 231 10 211 561 188 542 3.40e-33 134
MS.gene070122.t1 MTR_8g074040 29.319 382 231 11 211 568 108 474 4.20e-33 132
MS.gene070122.t1 MTR_0161s0050 28.649 370 234 10 209 561 188 544 4.49e-33 134
MS.gene070122.t1 MTR_5g008820 28.291 509 308 18 81 561 52 531 5.34e-33 133
MS.gene070122.t1 MTR_2g030240 26.325 547 323 16 72 568 31 547 1.99e-31 129
MS.gene070122.t1 MTR_8g074120 29.756 410 221 17 184 562 116 489 3.14e-31 127
MS.gene070122.t1 MTR_4g013210 26.790 377 216 9 210 567 69 404 1.91e-29 120
MS.gene070122.t1 MTR_5g076200 26.523 509 312 17 89 561 60 542 2.33e-29 122
MS.gene070122.t1 MTR_7g084850 24.464 560 367 15 44 568 186 724 5.59e-29 122
MS.gene070122.t1 MTR_4g013210 26.790 377 216 9 210 567 221 556 7.44e-29 121
MS.gene070122.t1 MTR_5g023730 26.706 513 324 20 81 566 55 542 3.77e-28 119
MS.gene070122.t1 MTR_4g013210 28.212 358 214 11 210 536 221 566 4.58e-28 119
MS.gene070122.t1 MTR_1g026350 41.045 134 79 0 21 154 20 153 7.12e-28 110
MS.gene070122.t1 MTR_2g030240 26.343 391 237 9 215 568 77 453 1.47e-25 110
MS.gene070122.t1 MTR_1g104810 26.909 550 319 20 3 485 35 568 2.18e-23 105
MS.gene070122.t1 MTR_3g087980 24.544 493 280 16 69 488 106 579 2.81e-23 104
MS.gene070122.t1 MTR_1g030290 24.900 498 272 19 69 488 108 581 1.65e-22 102
MS.gene070122.t1 MTR_1g104810 30.982 326 164 13 208 485 189 501 4.09e-22 100
MS.gene070122.t1 MTR_4g057755 55.556 72 32 0 424 495 46 117 6.90e-22 91.7
MS.gene070122.t1 MTR_5g009350 24.184 521 313 15 38 490 38 544 3.61e-21 97.8
MS.gene070122.t1 MTR_5g009350 24.184 521 313 15 38 490 38 544 3.75e-21 97.8
MS.gene070122.t1 MTR_5g009350 24.184 521 313 15 38 490 38 544 4.23e-21 97.4
MS.gene070122.t1 MTR_5g009350 24.184 521 313 15 38 490 38 544 4.40e-21 97.8
MS.gene070122.t1 MTR_1054s0020 24.453 503 344 14 66 562 294 766 3.41e-20 95.1
MS.gene070122.t1 MTR_7g109830 24.242 528 314 15 38 495 36 547 9.64e-19 90.5
MS.gene070122.t1 MTR_8g083170 31.818 220 128 7 339 545 461 671 3.39e-18 89.0
MS.gene070122.t1 MTR_2g098490 26.515 396 235 16 211 557 647 1035 4.60e-18 89.0
MS.gene070122.t1 MTR_3g082660 26.705 352 179 14 202 486 272 611 6.98e-18 87.8
MS.gene070122.t1 MTR_3g084450 35.673 171 87 7 328 481 233 397 2.43e-17 85.5
MS.gene070122.t1 MTR_3g084470 37.671 146 75 5 348 481 460 601 4.10e-17 85.5
MS.gene070122.t1 MTR_4g013210 27.018 285 158 8 210 485 221 464 5.60e-17 84.3
MS.gene070122.t1 MTR_3g084450 35.673 171 87 7 328 481 441 605 5.70e-17 85.1
MS.gene070122.t1 MTR_3g088890 48.438 64 33 0 470 533 10 73 1.32e-13 67.0
MS.gene070122.t1 MTR_4g013210 27.126 247 148 9 210 436 221 455 1.26e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070122.t1 AT4G19010 59.420 552 212 4 18 568 22 562 0.0 660
MS.gene070122.t1 AT1G20510 43.557 551 288 8 21 566 10 542 6.73e-158 462
MS.gene070122.t1 AT1G20480 42.934 559 288 9 21 568 27 565 1.29e-149 442
MS.gene070122.t1 AT1G20500 42.134 553 298 9 20 568 16 550 1.36e-141 421
MS.gene070122.t1 AT4G05160 41.016 551 305 8 24 568 8 544 3.46e-140 417
MS.gene070122.t1 AT5G38120 40.472 551 308 8 21 568 17 550 4.46e-135 404
MS.gene070122.t1 AT5G63380 39.820 555 298 9 21 560 17 550 1.67e-133 400
MS.gene070122.t1 AT1G20510 42.917 480 251 8 21 495 10 471 4.38e-132 394
MS.gene070122.t1 AT5G38120 39.612 515 291 8 21 532 17 514 1.71e-120 365
MS.gene070122.t1 AT1G20510 41.304 460 247 8 21 475 10 451 5.79e-119 360
MS.gene070122.t1 AT3G21240 40.691 521 278 10 59 568 54 554 1.49e-116 357
MS.gene070122.t1 AT5G38120 39.749 478 268 8 21 495 17 477 2.55e-110 338
MS.gene070122.t1 AT5G38120 39.749 478 268 8 21 495 17 477 2.55e-110 338
MS.gene070122.t1 AT1G51680 40.848 519 285 11 59 568 56 561 9.83e-108 334
MS.gene070122.t1 AT3G21230 38.336 553 314 9 27 566 28 566 2.71e-107 333
MS.gene070122.t1 AT1G65060 37.343 557 328 10 9 561 18 557 1.43e-100 316
MS.gene070122.t1 AT3G21230 38.150 519 294 9 27 532 28 532 2.66e-96 304
MS.gene070122.t1 AT1G51680 40.373 483 266 11 59 532 56 525 3.61e-95 301
MS.gene070122.t1 AT1G62940 35.727 557 311 11 27 566 12 538 2.75e-94 299
MS.gene070122.t1 AT3G21240 39.556 450 241 10 59 497 54 483 5.17e-92 291
MS.gene070122.t1 AT3G21240 39.556 450 241 10 59 497 54 483 5.17e-92 291
MS.gene070122.t1 AT1G20490 38.269 439 249 8 12 444 6 428 6.27e-92 290
MS.gene070122.t1 AT1G20490 38.269 439 249 8 12 444 6 428 6.27e-92 290
MS.gene070122.t1 AT3G21230 37.397 484 276 9 27 497 28 497 3.73e-86 276
MS.gene070122.t1 AT1G51680 39.286 448 250 11 59 497 56 490 9.84e-84 270
MS.gene070122.t1 AT1G65060 35.789 475 280 12 9 477 18 473 8.11e-70 233
MS.gene070122.t1 AT3G21230 32.446 413 252 9 27 426 28 426 1.10e-48 175
MS.gene070122.t1 AT3G48990 27.309 498 332 14 68 558 30 504 2.70e-43 162
MS.gene070122.t1 AT1G77240 30.132 531 309 21 68 566 43 543 2.76e-43 162
MS.gene070122.t1 AT1G21540 26.523 558 349 17 42 566 19 548 5.91e-42 159
MS.gene070122.t1 AT1G65880 28.544 522 311 18 81 562 52 551 3.48e-40 154
MS.gene070122.t1 AT5G16370 27.324 527 330 17 66 562 36 539 1.40e-39 152
MS.gene070122.t1 AT1G21530 27.338 556 338 22 42 562 19 543 1.94e-39 152
MS.gene070122.t1 AT1G21530 27.240 558 334 24 42 562 19 541 2.93e-39 151
MS.gene070122.t1 AT1G65890 27.495 531 304 18 81 562 52 550 3.91e-38 148
MS.gene070122.t1 AT1G20560 28.937 508 314 17 81 562 54 540 3.02e-37 145
MS.gene070122.t1 AT1G75960 27.866 506 320 15 81 561 53 538 1.41e-36 143
MS.gene070122.t1 AT3G16910 29.771 393 235 10 194 562 181 556 2.91e-36 143
MS.gene070122.t1 AT1G66120 28.906 512 314 16 81 562 52 543 3.30e-36 143
MS.gene070122.t1 AT5G16340 26.880 532 326 18 66 562 36 539 1.49e-35 140
MS.gene070122.t1 AT1G30520 27.473 546 295 19 78 562 42 547 8.72e-35 138
MS.gene070122.t1 AT1G30520 27.473 546 295 19 78 562 42 547 8.72e-35 138
MS.gene070122.t1 AT1G20560 31.842 380 226 14 198 561 99 461 3.18e-34 135
MS.gene070122.t1 AT2G17650 28.180 511 324 16 81 562 99 595 1.99e-33 135
MS.gene070122.t1 AT5G36880 25.281 534 357 14 67 568 146 669 2.35e-30 126
MS.gene070122.t1 AT5G36880 25.281 534 357 14 67 568 146 669 2.35e-30 126
MS.gene070122.t1 AT5G36880 25.281 534 357 14 67 568 146 669 2.35e-30 126
MS.gene070122.t1 AT5G36880 25.281 534 357 14 67 568 146 669 2.35e-30 126
MS.gene070122.t1 AT5G36880 25.468 534 356 14 67 568 196 719 2.91e-30 126
MS.gene070122.t1 AT3G16170 25.773 388 244 12 210 567 234 607 9.48e-30 124
MS.gene070122.t1 AT5G27600 26.568 542 315 16 7 481 42 567 2.37e-28 120
MS.gene070122.t1 AT1G30520 26.681 461 250 16 78 492 42 460 1.51e-26 113
MS.gene070122.t1 AT1G30520 26.681 461 250 16 78 492 42 460 1.51e-26 113
MS.gene070122.t1 AT5G36880 25.983 458 300 13 67 495 146 593 8.51e-25 109
MS.gene070122.t1 AT1G64400 25.194 516 306 16 32 483 39 538 1.17e-24 108
MS.gene070122.t1 AT1G76290 26.367 512 314 21 81 561 52 531 1.95e-24 107
MS.gene070122.t1 AT1G68270 36.709 158 89 2 414 561 363 519 1.16e-23 105
MS.gene070122.t1 AT4G11030 23.364 535 325 16 40 509 49 563 9.87e-22 99.8
MS.gene070122.t1 AT1G77590 26.943 386 197 14 211 531 100 465 1.61e-21 98.6
MS.gene070122.t1 AT4G23850 24.254 536 297 17 51 509 60 563 1.99e-21 99.0
MS.gene070122.t1 AT1G77590 26.943 386 197 14 211 531 246 611 2.39e-21 98.6
MS.gene070122.t1 AT3G05970 30.312 320 166 10 208 481 259 567 9.92e-21 96.7
MS.gene070122.t1 AT3G23790 34.932 146 79 5 348 481 465 606 1.01e-14 77.8
MS.gene070122.t1 AT2G04350 22.495 489 292 15 70 486 131 604 1.96e-14 77.0
MS.gene070122.t1 AT2G04350 22.495 489 292 15 70 486 131 604 1.96e-14 77.0
MS.gene070122.t1 AT4G14070 29.268 164 99 6 348 499 481 639 7.95e-11 65.5

Find 124 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTGGTCTTCATAAAA+TGG 0.181069 5.4:-18175325 MS.gene070122:CDS
ATGGGATAGTAAATTGAATT+TGG 0.204514 5.4:+18175267 None:intergenic
ATGACATTGATGAGGCTATT+AGG 0.229682 5.4:-18174696 MS.gene070122:CDS
TATCAAATACAAGGGCTTTC+AGG 0.237687 5.4:-18173628 MS.gene070122:intron
TTGCCGATGTTCCATTTATA+TGG 0.273927 5.4:-18174781 MS.gene070122:CDS
TAATCCTCATGACGGGGTTT+TGG 0.286770 5.4:-18175415 MS.gene070122:CDS
TTAGTCAAATCCTTGGCTTC+TGG 0.296472 5.4:-18175339 MS.gene070122:CDS
TGCTACCGGATTATTGTCAT+TGG 0.301910 5.4:-18174746 MS.gene070122:CDS
AGAGCGATAAACCATATAAA+TGG 0.308944 5.4:+18174770 None:intergenic
AAAGCTCACCGCTGAGGCCT+GGG 0.310423 5.4:+18174016 None:intergenic
CAAAGCTCACCGCTGAGGCC+TGG 0.333831 5.4:+18174015 None:intergenic
ACGGCGGCATCAACTATTTC+AGG 0.342047 5.4:+18173458 None:intergenic
ACTTTCCGAACCTTCTTGTA+TGG 0.345292 5.4:+18173099 None:intergenic
CAGGCCTCAGCGGTGAGCTT+TGG 0.348099 5.4:-18174014 MS.gene070122:CDS
ACACTATAATTTACCCTCAT+TGG 0.352827 5.4:-18175530 MS.gene070122:CDS
GATGGATATTTATATTTGTC+TGG 0.362112 5.4:-18173666 MS.gene070122:CDS
TAACAAATTTGAGTAAGAAA+TGG 0.376318 5.4:+18175365 None:intergenic
TGACATTGATGAGGCTATTA+GGG 0.377552 5.4:-18174695 MS.gene070122:CDS
ACCTGTATAAAATCAACATT+AGG 0.386845 5.4:+18174502 None:intergenic
GCTGGTATACTATATTCTTC+TGG 0.393544 5.4:-18174931 MS.gene070122:CDS
GTGTCTTCTTGTTTAATAAC+CGG 0.393788 5.4:+18174955 None:intergenic
GCCCTTGTTGATTCCACAGC+CGG 0.394912 5.4:-18175393 MS.gene070122:CDS
GGAGATTCCAGTGGCATTTG+TGG 0.394931 5.4:-18173318 MS.gene070122:CDS
GAAAATCAATGGCTACAAAC+TGG 0.395261 5.4:-18175575 None:intergenic
AATAGAATTTACCATAAGTA+AGG 0.398034 5.4:+18175025 None:intergenic
TGCTGTGCCAGAAAATGAAA+TGG 0.427789 5.4:-18175082 MS.gene070122:CDS
GCTTCTGGTCTTCATAAAAT+GGG 0.430989 5.4:-18175324 MS.gene070122:CDS
GTCAATGCCGACAACATCAT+TGG 0.431399 5.4:+18174634 None:intergenic
TGGAGGCAAAAGTGGTAGAT+TGG 0.438591 5.4:-18174059 MS.gene070122:CDS
AAAGATGGTTGGCTACATAC+TGG 0.442873 5.4:-18173711 MS.gene070122:CDS
GATCGATTACGTTGCTAAGC+AGG 0.446575 5.4:-18173258 MS.gene070122:intron
GGGTAAATTATAGTGTCGTT+GGG 0.447094 5.4:+18175537 None:intergenic
AAGGAAACAACGTCGAGAAA+AGG 0.447139 5.4:+18175447 None:intergenic
AGGGTAAATTATAGTGTCGT+TGG 0.448190 5.4:+18175536 None:intergenic
AGGAAACAACGTCGAGAAAA+GGG 0.453163 5.4:+18175448 None:intergenic
GCTACCGGATTATTGTCATT+GGG 0.459661 5.4:-18174745 MS.gene070122:CDS
AGTGGCAAGGTTGATGAAGC+TGG 0.461000 5.4:-18173341 MS.gene070122:CDS
GGGTCGGTTGGAAGGTTCCT+AGG 0.464056 5.4:+18175468 None:intergenic
TGTTTAATAACCGGCCTTTG+AGG 0.466941 5.4:+18174964 None:intergenic
ATAACACTCAATAGTACAAT+GGG 0.478275 5.4:+18175249 None:intergenic
CTTTGATTTGCCTCAAAGGC+CGG 0.478557 5.4:-18174974 MS.gene070122:CDS
CTATATTCTTCTGGTACTAC+AGG 0.481197 5.4:-18174922 MS.gene070122:CDS
AAGATACCGAGATCTGCAAC+TGG 0.482468 5.4:-18173063 MS.gene070122:CDS
GGTTTATCGCTCTTTGCTAC+CGG 0.483340 5.4:-18174760 MS.gene070122:CDS
AAGCTCACCGCTGAGGCCTG+GGG 0.483974 5.4:+18174017 None:intergenic
TTCTTGACCACAAATGCCAC+TGG 0.484108 5.4:+18173311 None:intergenic
ATTTGATAATGTCTTTCAAA+CGG 0.487340 5.4:+18173643 None:intergenic
TCATAGAAATCTTATTGCTA+TGG 0.487900 5.4:-18174875 MS.gene070122:CDS
GTTAACTTTAGTCAAATCCT+TGG 0.487963 5.4:-18175346 MS.gene070122:CDS
CGAGAAAAGGGTCGGTTGGA+AGG 0.488044 5.4:+18175460 None:intergenic
TTTCATAACTATTCCTCACT+AGG 0.488335 5.4:-18174099 MS.gene070122:CDS
AACAAAGCTTCTAAATCAGC+AGG 0.490080 5.4:+18173491 None:intergenic
ATTTCGCTAACACTACTAAG+AGG 0.492190 5.4:+18175195 None:intergenic
AGCGGTGAGCTTTGGTTGAG+AGG 0.492979 5.4:-18174006 MS.gene070122:CDS
AACAGCTAGATGCACAGTCT+TGG 0.496252 5.4:+18174804 None:intergenic
ACAGCAGTTTACAACCTCCT+AGG 0.498653 5.4:-18175485 MS.gene070122:CDS
TTGCTCCATACAAGAAGGTT+CGG 0.499371 5.4:-18173104 MS.gene070122:CDS
AAATCCTCCGACAGCAACTC+AGG 0.499555 5.4:-18173038 MS.gene070122:CDS
CTATTGAGTGTTATGTATCT+AGG 0.499995 5.4:-18175240 MS.gene070122:CDS
TGTTAGCGAAATTCGTAAGC+AGG 0.502850 5.4:-18175184 MS.gene070122:CDS
AAAGTGGTAGATTGGAAAAG+TGG 0.504015 5.4:-18174051 MS.gene070122:CDS
AATCAACATTAGGAAAAGCT+TGG 0.504448 5.4:+18174512 None:intergenic
TGTTGTTCCAATGATGTTGT+CGG 0.504839 5.4:-18174641 MS.gene070122:CDS
ATCAAGAAGTTAGAGTCATT+AGG 0.508091 5.4:-18175117 MS.gene070122:CDS
GGTTATGGAATGACTGAGTC+GGG 0.510519 5.4:-18174156 MS.gene070122:CDS
GCGGTGAGCTTTGGTTGAGA+GGG 0.514715 5.4:-18174005 MS.gene070122:CDS
GAAAGACATTATCAAATACA+AGG 0.519394 5.4:-18173637 MS.gene070122:CDS
ATGTCAACAATTGATAAAGA+TGG 0.520159 5.4:-18173726 MS.gene070122:CDS
ACACTACTAAGAGGATTCAA+TGG 0.522819 5.4:+18175204 None:intergenic
TTCATATACTTACGCTGTAA+CGG 0.523614 5.4:+18173439 None:intergenic
TTTATAGCTCCTTTGCTCAA+AGG 0.526141 5.4:+18174544 None:intergenic
CCCTTGTTGATTCCACAGCC+GGG 0.527853 5.4:-18175392 MS.gene070122:CDS
TTCATAACTATTCCTCACTA+GGG 0.528159 5.4:-18174098 MS.gene070122:CDS
GTGGCAAGGTTGATGAAGCT+GGG 0.529856 5.4:-18173340 MS.gene070122:CDS
TCCAAACATGGAGGCAAAAG+TGG 0.536470 5.4:-18174067 MS.gene070122:CDS
CATAAAATGGGTGTTTCACA+AGG 0.537350 5.4:-18175312 MS.gene070122:CDS
GGGTTATGGAATGACTGAGT+CGG 0.541532 5.4:-18174157 MS.gene070122:CDS
GGTGCTGAGCCTTTGAGCAA+AGG 0.542374 5.4:-18174553 MS.gene070122:CDS
ATACAAGAAGGTTCGGAAAG+TGG 0.543810 5.4:-18173097 MS.gene070122:CDS
GTGACTTTGATTTGCCTCAA+AGG 0.544155 5.4:-18174978 MS.gene070122:CDS
AGAACCCAATGACAATAATC+CGG 0.547525 5.4:+18174741 None:intergenic
ATGACTGAGTCGGGCGCAGT+AGG 0.553182 5.4:-18174147 MS.gene070122:CDS
AGAGGGCCTTCAATTATGAA+AGG 0.572900 5.4:-18173988 MS.gene070122:intron
CAACAATTGATAAAGATGGT+TGG 0.574233 5.4:-18173722 MS.gene070122:CDS
AGAAATGGAAGACCCGGCTG+TGG 0.575962 5.4:+18175380 None:intergenic
CATAACACTCAATAGTACAA+TGG 0.577708 5.4:+18175248 None:intergenic
AGCTCACCGCTGAGGCCTGG+GGG 0.583783 5.4:+18174018 None:intergenic
AAAGACATTATCAAATACAA+GGG 0.586533 5.4:-18173636 MS.gene070122:CDS
AAACCATATAAATGGAACAT+CGG 0.588130 5.4:+18174778 None:intergenic
CGTAGGATACTTGAATAATG+AGG 0.588911 5.4:-18173757 MS.gene070122:intron
TTTATGAAGACCAGAAGCCA+AGG 0.591784 5.4:+18175329 None:intergenic
GCATTTGTGGTCAAGAAAGT+CGG 0.593489 5.4:-18173305 MS.gene070122:CDS
ATATACTTACGCTGTAACGG+CGG 0.594057 5.4:+18173442 None:intergenic
ACGTCGAGAAAAGGGTCGGT+TGG 0.599763 5.4:+18175456 None:intergenic
GGCATTGACAGCGAAGGCAA+AGG 0.603341 5.4:-18174620 MS.gene070122:CDS
CGTAAGCAGGTTAAAGAGTG+TGG 0.604728 5.4:-18175171 MS.gene070122:CDS
AGTTTAAGACATGTCTCTTG+CGG 0.607812 5.4:-18174574 MS.gene070122:CDS
ACCCGGCTGTGGAATCAACA+AGG 0.610400 5.4:+18175391 None:intergenic
TGAGTAAGAAATGGAAGACC+CGG 0.611775 5.4:+18175374 None:intergenic
AGGGTTGTTAGCTCCAAACA+TGG 0.614345 5.4:-18174079 MS.gene070122:CDS
AGGGCCAAAACCCCGTCATG+AGG 0.614534 5.4:+18175411 None:intergenic
AGCAATTCCTGAGTTGCTGT+CGG 0.617746 5.4:+18173031 None:intergenic
GGTACTACAGGTGTGAGCAA+AGG 0.618393 5.4:-18174910 MS.gene070122:CDS
AATTCCTGAGTTGCTGTCGG+AGG 0.618818 5.4:+18173034 None:intergenic
TTGACAGCGAAGGCAAAGGA+TGG 0.620619 5.4:-18174616 MS.gene070122:CDS
ATTAAACAAGAAGACACTGC+TGG 0.621260 5.4:-18174949 MS.gene070122:CDS
GCAGGTTGCTCCATACAAGA+AGG 0.622353 5.4:-18173109 MS.gene070122:intron
TGGCAAGGTTGATGAAGCTG+GGG 0.643596 5.4:-18173339 MS.gene070122:CDS
TGGAGCTAACAACCCTAGTG+AGG 0.644923 5.4:+18174086 None:intergenic
ACAACATCATTGGAACAACA+TGG 0.657828 5.4:+18174644 None:intergenic
AGATACCGAGATCTGCAACT+GGG 0.658351 5.4:-18173062 MS.gene070122:CDS
GTTGTCGGCATTGACAGCGA+AGG 0.659388 5.4:-18174626 MS.gene070122:CDS
TGCTTGACTTCTAAGCTCTG+AGG 0.664251 5.4:-18173012 None:intergenic
TCGGTTGGAAGGTTCCTAGG+AGG 0.668587 5.4:+18175471 None:intergenic
GCGGAAATATGACATTGATG+AGG 0.669164 5.4:-18174704 MS.gene070122:CDS
GTTCTACAATCGTTGTAATG+CGG 0.673134 5.4:-18174723 MS.gene070122:CDS
GGTAAATTATAGTGTCGTTG+GGG 0.675783 5.4:+18175538 None:intergenic
AGGATTCAATGGTGTAACAA+CGG 0.680938 5.4:+18175215 None:intergenic
TCAACCAAAGCTCACCGCTG+AGG 0.681517 5.4:+18174010 None:intergenic
AACAACGTCGAGAAAAGGGT+CGG 0.682246 5.4:+18175452 None:intergenic
TCGGGCGCAGTAGGAACACG+CGG 0.689165 5.4:-18174138 MS.gene070122:CDS
TGAAGCTGGGGAGATTCCAG+TGG 0.700734 5.4:-18173327 MS.gene070122:CDS
CCCGGCTGTGGAATCAACAA+GGG 0.701999 5.4:+18175392 None:intergenic
GTTGTTAGCTCCAAACATGG+AGG 0.741487 5.4:-18174076 MS.gene070122:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATCTGTATTTTTATA+AGG - chr5.4:18174617-18174636 MS.gene070122:CDS 10.0%
!!! ATAAAGAAAAATCTTATTTA+CGG - chr5.4:18173581-18173600 MS.gene070122:intron 10.0%
!!! ATGAAATAAAGGAAAAATTT+TGG + chr5.4:18174800-18174819 None:intergenic 15.0%
!!! TAAACTTTTTTTTTTTTTCC+TGG - chr5.4:18175450-18175469 MS.gene070122:CDS 15.0%
!! AAAGACATTATCAAATACAA+GGG - chr5.4:18174945-18174964 MS.gene070122:CDS 20.0%
!! AATAGAATTTACCATAAGTA+AGG + chr5.4:18173559-18173578 None:intergenic 20.0%
!! TAACAAATTTGAGTAAGAAA+TGG + chr5.4:18173219-18173238 None:intergenic 20.0%
!!! ATTGTTTATTTTGATCAAGA+TGG - chr5.4:18174897-18174916 MS.gene070122:CDS 20.0%
!!! ATTTGATAATGTCTTTCAAA+CGG + chr5.4:18174941-18174960 None:intergenic 20.0%
!!! TGTATGATTGTTTCTTTTTA+GGG - chr5.4:18174404-18174423 MS.gene070122:intron 20.0%
!!! TGTTTTTATAACCTTACTTA+TGG - chr5.4:18173545-18173564 MS.gene070122:intron 20.0%
!!! TTTTGGTTAAGAATTTCAAA+TGG + chr5.4:18174783-18174802 None:intergenic 20.0%
!!! TTTTTACCTTTCATAATTGA+AGG + chr5.4:18174602-18174621 None:intergenic 20.0%
! AAACCATATAAATGGAACAT+CGG + chr5.4:18173806-18173825 None:intergenic 25.0%
! ACCTGTATAAAATCAACATT+AGG + chr5.4:18174082-18174101 None:intergenic 25.0%
! AGTTGAACTTTACTTGTTTA+TGG - chr5.4:18174342-18174361 MS.gene070122:intron 25.0%
! ATAACACTCAATAGTACAAT+GGG + chr5.4:18173335-18173354 None:intergenic 25.0%
! ATGAGGATTAGAAAAAATGA+AGG + chr5.4:18173156-18173175 None:intergenic 25.0%
! ATGGGATAGTAAATTGAATT+TGG + chr5.4:18173317-18173336 None:intergenic 25.0%
! ATGTCAACAATTGATAAAGA+TGG - chr5.4:18174855-18174874 MS.gene070122:CDS 25.0%
! CAAGTAAAGTTCAACTATTA+TGG + chr5.4:18174338-18174357 None:intergenic 25.0%
! GAAAGACATTATCAAATACA+AGG - chr5.4:18174944-18174963 MS.gene070122:CDS 25.0%
! GATGGATATTTATATTTGTC+TGG - chr5.4:18174915-18174934 MS.gene070122:CDS 25.0%
! TCATAGAAATCTTATTGCTA+TGG - chr5.4:18173706-18173725 MS.gene070122:CDS 25.0%
! TGTGAATATGAATACCATAA+CGG + chr5.4:18174303-18174322 None:intergenic 25.0%
!! CTAATTTTCTTTTTTCTCCA+AGG + chr5.4:18174111-18174130 None:intergenic 25.0%
!! CTCTAACTTCTTGATATTTT+CGG + chr5.4:18173459-18173478 None:intergenic 25.0%
!! TCTAACTTCTTGATATTTTC+GGG + chr5.4:18173458-18173477 None:intergenic 25.0%
!! TTCAAATCCATTTCATTTTC+TGG + chr5.4:18173509-18173528 None:intergenic 25.0%
!! TTTTTGTTGAATACCAATGA+GGG + chr5.4:18173067-18173086 None:intergenic 25.0%
!!! ATTGTTTCTTTTTAGGGTTA+TGG - chr5.4:18174410-18174429 MS.gene070122:intron 25.0%
!!! ATTTTTTCTAATCCTCATGA+CGG - chr5.4:18173158-18173177 MS.gene070122:intron 25.0%
!!! CTGTATGATTGTTTCTTTTT+AGG - chr5.4:18174403-18174422 MS.gene070122:intron 25.0%
!!! GTTGATTTCCTAGATTTTTA+AGG - chr5.4:18174372-18174391 MS.gene070122:intron 25.0%
!!! TCATATTTTAATGTCCAAAG+AGG + chr5.4:18175192-18175211 None:intergenic 25.0%
!!! TCCTAATGTTGATTTTATAC+AGG - chr5.4:18174078-18174097 MS.gene070122:CDS 25.0%
!!! TTTTCTTTGTAATTGCTGTT+AGG - chr5.4:18174186-18174205 MS.gene070122:intron 25.0%
AATCAACATTAGGAAAAGCT+TGG + chr5.4:18174072-18174091 None:intergenic 30.0%
AATCAATCTCATGCAAATGT+AGG + chr5.4:18174668-18174687 None:intergenic 30.0%
ACACTATAATTTACCCTCAT+TGG - chr5.4:18173051-18173070 MS.gene070122:CDS 30.0%
AGAGCGATAAACCATATAAA+TGG + chr5.4:18173814-18173833 None:intergenic 30.0%
AGTGTCTATTACTATTGGAA+TGG - chr5.4:18175416-18175435 MS.gene070122:CDS 30.0%
ATCAAGAAGTTAGAGTCATT+AGG - chr5.4:18173464-18173483 MS.gene070122:CDS 30.0%
CAACAATTGATAAAGATGGT+TGG - chr5.4:18174859-18174878 MS.gene070122:CDS 30.0%
CATAACACTCAATAGTACAA+TGG + chr5.4:18173336-18173355 None:intergenic 30.0%
CATTGGTATTCAACAAAAAC+TGG - chr5.4:18173068-18173087 MS.gene070122:CDS 30.0%
CTATTGAGTGTTATGTATCT+AGG - chr5.4:18173341-18173360 MS.gene070122:CDS 30.0%
CTTCACATCCTTAAAAATCT+AGG + chr5.4:18174383-18174402 None:intergenic 30.0%
GTATGCATTGTTCTTATGTA+TGG - chr5.4:18175044-18175063 MS.gene070122:CDS 30.0%
GTCATAGTGTCTATTACTAT+TGG - chr5.4:18175411-18175430 MS.gene070122:CDS 30.0%
GTTAACTTTAGTCAAATCCT+TGG - chr5.4:18173235-18173254 MS.gene070122:intron 30.0%
TCCTACGAAGTATGAAATAA+AGG + chr5.4:18174811-18174830 None:intergenic 30.0%
TTCATAACTATTCCTCACTA+GGG - chr5.4:18174483-18174502 MS.gene070122:intron 30.0%
TTCATATACTTACGCTGTAA+CGG + chr5.4:18175145-18175164 None:intergenic 30.0%
TTCATATTCACAACATGTTC+TGG - chr5.4:18174311-18174330 MS.gene070122:intron 30.0%
TTTATACAGGTAAAGAACCT+TGG - chr5.4:18174091-18174110 MS.gene070122:CDS 30.0%
TTTCATAACTATTCCTCACT+AGG - chr5.4:18174482-18174501 MS.gene070122:intron 30.0%
! GTGTCTTCTTGTTTAATAAC+CGG + chr5.4:18173629-18173648 None:intergenic 30.0%
! GTTTTTGTTGAATACCAATG+AGG + chr5.4:18173068-18173087 None:intergenic 30.0%
! TCCTTTATTTCATACTTCGT+AGG - chr5.4:18174807-18174826 MS.gene070122:CDS 30.0%
! TGACTTGCGTTCTTTAATTA+AGG + chr5.4:18174134-18174153 None:intergenic 30.0%
!!! CTTTTTTTTTTTTTCCTGGC+AGG - chr5.4:18175454-18175473 MS.gene070122:CDS 30.0%
!!! TTTTTTCTAATCCTCATGAC+GGG - chr5.4:18173159-18173178 MS.gene070122:intron 30.0%
AAAGTGGTAGATTGGAAAAG+TGG - chr5.4:18174530-18174549 MS.gene070122:CDS 35.0%
AACAAAGCTTCTAAATCAGC+AGG + chr5.4:18175093-18175112 None:intergenic 35.0%
AATGGAATAGCAAAAGAGAC+AGG + chr5.4:18174765-18174784 None:intergenic 35.0%
ACAACATCATTGGAACAACA+TGG + chr5.4:18173940-18173959 None:intergenic 35.0%
ACACTACTAAGAGGATTCAA+TGG + chr5.4:18173380-18173399 None:intergenic 35.0%
AGAACCCAATGACAATAATC+CGG + chr5.4:18173843-18173862 None:intergenic 35.0%
AGGATTCAATGGTGTAACAA+CGG + chr5.4:18173369-18173388 None:intergenic 35.0%
AGGGTAAATTATAGTGTCGT+TGG + chr5.4:18173048-18173067 None:intergenic 35.0%
ATGACATTGATGAGGCTATT+AGG - chr5.4:18173885-18173904 MS.gene070122:intron 35.0%
ATTAAACAAGAAGACACTGC+TGG - chr5.4:18173632-18173651 MS.gene070122:CDS 35.0%
ATTTCGCTAACACTACTAAG+AGG + chr5.4:18173389-18173408 None:intergenic 35.0%
CAAAAGAGACAGGCAAAAAA+AGG + chr5.4:18174755-18174774 None:intergenic 35.0%
CATAAAATGGGTGTTTCACA+AGG - chr5.4:18173269-18173288 MS.gene070122:CDS 35.0%
CGAAAATGATGTGATACTCA+AGG + chr5.4:18175215-18175234 None:intergenic 35.0%
CGTAGGATACTTGAATAATG+AGG - chr5.4:18174824-18174843 MS.gene070122:CDS 35.0%
CTATATTCTTCTGGTACTAC+AGG - chr5.4:18173659-18173678 MS.gene070122:CDS 35.0%
GCTGGTATACTATATTCTTC+TGG - chr5.4:18173650-18173669 MS.gene070122:CDS 35.0%
GTTAGGAGCTAAATAAATGC+TGG - chr5.4:18174203-18174222 MS.gene070122:intron 35.0%
GTTCTACAATCGTTGTAATG+CGG - chr5.4:18173858-18173877 MS.gene070122:intron 35.0%
TATCAAATACAAGGGCTTTC+AGG - chr5.4:18174953-18174972 MS.gene070122:CDS 35.0%
TGACATTGATGAGGCTATTA+GGG - chr5.4:18173886-18173905 MS.gene070122:intron 35.0%
TGTTGTTCCAATGATGTTGT+CGG - chr5.4:18173940-18173959 MS.gene070122:intron 35.0%
TTGCCGATGTTCCATTTATA+TGG - chr5.4:18173800-18173819 MS.gene070122:intron 35.0%
TTTATAGCTCCTTTGCTCAA+AGG + chr5.4:18174040-18174059 None:intergenic 35.0%
! AAAAAGGTACAAGTAGGCTA+AGG + chr5.4:18174739-18174758 None:intergenic 35.0%
! AGGCAAAAAAAGGTACAAGT+AGG + chr5.4:18174745-18174764 None:intergenic 35.0%
! AGTTTAAGACATGTCTCTTG+CGG - chr5.4:18174007-18174026 MS.gene070122:CDS 35.0%
! CACATCATTTTCGATATCAG+TGG - chr5.4:18175222-18175241 MS.gene070122:CDS 35.0%
! CTATGGTTGAGCTTTTTATG+AGG - chr5.4:18173723-18173742 MS.gene070122:CDS 35.0%
! GCTTCTGGTCTTCATAAAAT+GGG - chr5.4:18173257-18173276 MS.gene070122:intron 35.0%
! GGGTAAATTATAGTGTCGTT+GGG + chr5.4:18173047-18173066 None:intergenic 35.0%
! GGTAAATTATAGTGTCGTTG+GGG + chr5.4:18173046-18173065 None:intergenic 35.0%
! TTTTTCTAATCCTCATGACG+GGG - chr5.4:18173160-18173179 MS.gene070122:intron 35.0%
AAGGAAACAACGTCGAGAAA+AGG + chr5.4:18173137-18173156 None:intergenic 40.0%
ACTTTCCGAACCTTCTTGTA+TGG + chr5.4:18175485-18175504 None:intergenic 40.0%
AGAGGGCCTTCAATTATGAA+AGG - chr5.4:18174593-18174612 MS.gene070122:CDS 40.0%
AGGAAACAACGTCGAGAAAA+GGG + chr5.4:18173136-18173155 None:intergenic 40.0%
ATACAAGAAGGTTCGGAAAG+TGG - chr5.4:18175484-18175503 MS.gene070122:CDS 40.0%
ATATACTTACGCTGTAACGG+CGG + chr5.4:18175142-18175161 None:intergenic 40.0%
GCATTTGTGGTCAAGAAAGT+CGG - chr5.4:18175276-18175295 MS.gene070122:CDS 40.0%
GCGGAAATATGACATTGATG+AGG - chr5.4:18173877-18173896 MS.gene070122:intron 40.0%
GCTACCGGATTATTGTCATT+GGG - chr5.4:18173836-18173855 MS.gene070122:intron 40.0%
TGAGTAAGAAATGGAAGACC+CGG + chr5.4:18173210-18173229 None:intergenic 40.0%
TGCTACCGGATTATTGTCAT+TGG - chr5.4:18173835-18173854 MS.gene070122:intron 40.0%
TGCTGTGCCAGAAAATGAAA+TGG - chr5.4:18173499-18173518 MS.gene070122:CDS 40.0%
TGTTAGCGAAATTCGTAAGC+AGG - chr5.4:18173397-18173416 MS.gene070122:intron 40.0%
TGTTTAATAACCGGCCTTTG+AGG + chr5.4:18173620-18173639 None:intergenic 40.0%
TTAGTCAAATCCTTGGCTTC+TGG - chr5.4:18173242-18173261 MS.gene070122:intron 40.0%
TTGCTCCATACAAGAAGGTT+CGG - chr5.4:18175477-18175496 MS.gene070122:CDS 40.0%
TTTATGAAGACCAGAAGCCA+AGG + chr5.4:18173255-18173274 None:intergenic 40.0%
! AAAGATGGTTGGCTACATAC+TGG - chr5.4:18174870-18174889 MS.gene070122:CDS 40.0%
! ACACTATTTCTTCGCCGTTA+TGG - chr5.4:18174286-18174305 MS.gene070122:intron 40.0%
! ATTTTCCCAGTTGCAGATCT+CGG + chr5.4:18175527-18175546 None:intergenic 40.0%
! CATTTTCGATATCAGTGGCA+AGG - chr5.4:18175227-18175246 MS.gene070122:CDS 40.0%
! GGCTTCTGGTCTTCATAAAA+TGG - chr5.4:18173256-18173275 MS.gene070122:intron 40.0%
!! GTGACTTTGATTTGCCTCAA+AGG - chr5.4:18173603-18173622 MS.gene070122:intron 40.0%
!! TGTCTTCTAAAGTGCCTCTT+TGG - chr5.4:18175175-18175194 MS.gene070122:CDS 40.0%
!! TTTTCTGGCACAGCAATAAC+AGG + chr5.4:18173494-18173513 None:intergenic 40.0%
AACAACGTCGAGAAAAGGGT+CGG + chr5.4:18173132-18173151 None:intergenic 45.0%
AACAGCTAGATGCACAGTCT+TGG + chr5.4:18173780-18173799 None:intergenic 45.0%
AAGATACCGAGATCTGCAAC+TGG - chr5.4:18175518-18175537 MS.gene070122:CDS 45.0%
ACAGCAGTTTACAACCTCCT+AGG - chr5.4:18173096-18173115 MS.gene070122:CDS 45.0%
AGATACCGAGATCTGCAACT+GGG - chr5.4:18175519-18175538 MS.gene070122:CDS 45.0%
AGCAATTCCTGAGTTGCTGT+CGG + chr5.4:18175553-18175572 None:intergenic 45.0%
AGGGTTGTTAGCTCCAAACA+TGG - chr5.4:18174502-18174521 MS.gene070122:intron 45.0%
CGTAAGCAGGTTAAAGAGTG+TGG - chr5.4:18173410-18173429 MS.gene070122:intron 45.0%
GATCGATTACGTTGCTAAGC+AGG - chr5.4:18175323-18175342 MS.gene070122:CDS 45.0%
GGGTTATGGAATGACTGAGT+CGG - chr5.4:18174424-18174443 MS.gene070122:intron 45.0%
GGTTATGGAATGACTGAGTC+GGG - chr5.4:18174425-18174444 MS.gene070122:intron 45.0%
GGTTTATCGCTCTTTGCTAC+CGG - chr5.4:18173821-18173840 MS.gene070122:intron 45.0%
GTCAATGCCGACAACATCAT+TGG + chr5.4:18173950-18173969 None:intergenic 45.0%
GTTGTTAGCTCCAAACATGG+AGG - chr5.4:18174505-18174524 MS.gene070122:CDS 45.0%
TCCAAACATGGAGGCAAAAG+TGG - chr5.4:18174514-18174533 MS.gene070122:CDS 45.0%
TGGAGGCAAAAGTGGTAGAT+TGG - chr5.4:18174522-18174541 MS.gene070122:CDS 45.0%
TTCTTGACCACAAATGCCAC+TGG + chr5.4:18175273-18175292 None:intergenic 45.0%
!! ACCACTTTTGCCTCCATGTT+TGG + chr5.4:18174518-18174537 None:intergenic 45.0%
!! CTTTGATTTGCCTCAAAGGC+CGG - chr5.4:18173607-18173626 MS.gene070122:intron 45.0%
!! TAATCCTCATGACGGGGTTT+TGG - chr5.4:18173166-18173185 MS.gene070122:intron 45.0%
AAATCCTCCGACAGCAACTC+AGG - chr5.4:18175543-18175562 MS.gene070122:CDS 50.0%
AATTCCTGAGTTGCTGTCGG+AGG + chr5.4:18175550-18175569 None:intergenic 50.0%
ACGGCGGCATCAACTATTTC+AGG + chr5.4:18175126-18175145 None:intergenic 50.0%
GCAGGTTGCTCCATACAAGA+AGG - chr5.4:18175472-18175491 MS.gene070122:CDS 50.0%
GGAGATTCCAGTGGCATTTG+TGG - chr5.4:18175263-18175282 MS.gene070122:CDS 50.0%
GGTACTACAGGTGTGAGCAA+AGG - chr5.4:18173671-18173690 MS.gene070122:CDS 50.0%
TGGAGCTAACAACCCTAGTG+AGG + chr5.4:18174498-18174517 None:intergenic 50.0%
TTGACAGCGAAGGCAAAGGA+TGG - chr5.4:18173965-18173984 MS.gene070122:intron 50.0%
! AGTGGTAAGTTTTTGCCCCC+AGG - chr5.4:18174548-18174567 MS.gene070122:CDS 50.0%
!! AGTGGCAAGGTTGATGAAGC+TGG - chr5.4:18175240-18175259 MS.gene070122:CDS 50.0%
!! GTGGCAAGGTTGATGAAGCT+GGG - chr5.4:18175241-18175260 MS.gene070122:CDS 50.0%
!! TGGCAAGGTTGATGAAGCTG+GGG - chr5.4:18175242-18175261 MS.gene070122:CDS 50.0%
ACCCGGCTGTGGAATCAACA+AGG + chr5.4:18173193-18173212 None:intergenic 55.0%
ACGTCGAGAAAAGGGTCGGT+TGG + chr5.4:18173128-18173147 None:intergenic 55.0%
AGAAATGGAAGACCCGGCTG+TGG + chr5.4:18173204-18173223 None:intergenic 55.0%
CCCGGCTGTGGAATCAACAA+GGG + chr5.4:18173192-18173211 None:intergenic 55.0%
CGAGAAAAGGGTCGGTTGGA+AGG + chr5.4:18173124-18173143 None:intergenic 55.0%
CTTGTATGGAGCAACCTGCC+AGG + chr5.4:18175471-18175490 None:intergenic 55.0%
TCAACCAAAGCTCACCGCTG+AGG + chr5.4:18174574-18174593 None:intergenic 55.0%
TCGGTTGGAAGGTTCCTAGG+AGG + chr5.4:18173113-18173132 None:intergenic 55.0%
TGAAGCTGGGGAGATTCCAG+TGG - chr5.4:18175254-18175273 MS.gene070122:CDS 55.0%
! AGCGGTGAGCTTTGGTTGAG+AGG - chr5.4:18174575-18174594 MS.gene070122:CDS 55.0%
! GCGGTGAGCTTTGGTTGAGA+GGG - chr5.4:18174576-18174595 MS.gene070122:CDS 55.0%
! GGTGCTGAGCCTTTGAGCAA+AGG - chr5.4:18174028-18174047 MS.gene070122:CDS 55.0%
!! CCCTTGTTGATTCCACAGCC+GGG - chr5.4:18173189-18173208 MS.gene070122:intron 55.0%
!! GCCCTTGTTGATTCCACAGC+CGG - chr5.4:18173188-18173207 MS.gene070122:intron 55.0%
!! GGCATTGACAGCGAAGGCAA+AGG - chr5.4:18173961-18173980 MS.gene070122:intron 55.0%
!! GTTGTCGGCATTGACAGCGA+AGG - chr5.4:18173955-18173974 MS.gene070122:intron 55.0%
AAAGCTCACCGCTGAGGCCT+GGG + chr5.4:18174568-18174587 None:intergenic 60.0%
AGGGCCAAAACCCCGTCATG+AGG + chr5.4:18173173-18173192 None:intergenic 60.0%
ATGACTGAGTCGGGCGCAGT+AGG - chr5.4:18174434-18174453 MS.gene070122:intron 60.0%
GGGTCGGTTGGAAGGTTCCT+AGG + chr5.4:18173116-18173135 None:intergenic 60.0%
! TTTTTGCCCCCAGGCCTCAG+CGG - chr5.4:18174557-18174576 MS.gene070122:CDS 60.0%
AAGCTCACCGCTGAGGCCTG+GGG + chr5.4:18174567-18174586 None:intergenic 65.0%
CAAAGCTCACCGCTGAGGCC+TGG + chr5.4:18174569-18174588 None:intergenic 65.0%
CAGGCCTCAGCGGTGAGCTT+TGG - chr5.4:18174567-18174586 MS.gene070122:CDS 65.0%
TCGGGCGCAGTAGGAACACG+CGG - chr5.4:18174443-18174462 MS.gene070122:intron 65.0%
AGCTCACCGCTGAGGCCTGG+GGG + chr5.4:18174566-18174585 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 18173014 18175589 18173014 ID=MS.gene070122
chr5.4 mRNA 18173014 18175589 18173014 ID=MS.gene070122.t1;Parent=MS.gene070122
chr5.4 exon 18174504 18175589 18174504 ID=MS.gene070122.t1.exon1;Parent=MS.gene070122.t1
chr5.4 CDS 18174504 18175589 18174504 ID=cds.MS.gene070122.t1;Parent=MS.gene070122.t1
chr5.4 exon 18173989 18174178 18173989 ID=MS.gene070122.t1.exon2;Parent=MS.gene070122.t1
chr5.4 CDS 18173989 18174178 18173989 ID=cds.MS.gene070122.t1;Parent=MS.gene070122.t1
chr5.4 exon 18173629 18173774 18173629 ID=MS.gene070122.t1.exon3;Parent=MS.gene070122.t1
chr5.4 CDS 18173629 18173774 18173629 ID=cds.MS.gene070122.t1;Parent=MS.gene070122.t1
chr5.4 exon 18173452 18173519 18173452 ID=MS.gene070122.t1.exon4;Parent=MS.gene070122.t1
chr5.4 CDS 18173452 18173519 18173452 ID=cds.MS.gene070122.t1;Parent=MS.gene070122.t1
chr5.4 exon 18173259 18173361 18173259 ID=MS.gene070122.t1.exon5;Parent=MS.gene070122.t1
chr5.4 CDS 18173259 18173361 18173259 ID=cds.MS.gene070122.t1;Parent=MS.gene070122.t1
chr5.4 exon 18173014 18173127 18173014 ID=MS.gene070122.t1.exon6;Parent=MS.gene070122.t1
chr5.4 CDS 18173014 18173127 18173014 ID=cds.MS.gene070122.t1;Parent=MS.gene070122.t1
Gene Sequence

>MS.gene070122

ATGGCTACAAACTGGAATTTTTTTTCTGTCCCCAACGACACTATAATTTACCCTCATTGGTATTCAACAAAAACTGGAATTTACAGCAGTTTACAACCTCCTAGGAACCTTCCAACCGACCCTTTTCTCGACGTTGTTTCCTTCATTTTTTCTAATCCTCATGACGGGGTTTTGGCCCTTGTTGATTCCACAGCCGGGTCTTCCATTTCTTACTCAAATTTGTTAACTTTAGTCAAATCCTTGGCTTCTGGTCTTCATAAAATGGGTGTTTCACAAGGTGATGTTGTTTTGCTTTTACTTCCAAATTCAATTTACTATCCCATTGTACTATTGAGTGTTATGTATCTAGGTGCCGTTGTTACACCATTGAATCCTCTTAGTAGTGTTAGCGAAATTCGTAAGCAGGTTAAAGAGTGTGGTGTGAGCTTTGCTTTTACTATACCCGAAAATATCAAGAAGTTAGAGTCATTAGGCATTCCTGTTATTGCTGTGCCAGAAAATGAAATGGATTTGAAGCATGATTGTTTCTCATGTTTTTATAACCTTACTTATGGTAAATTCTATTCAATAAAGAAAAATCTTATTTACGGTGACTTTGATTTGCCTCAAAGGCCGGTTATTAAACAAGAAGACACTGCTGGTATACTATATTCTTCTGGTACTACAGGTGTGAGCAAAGGAGTTGTTTTAACTCATAGAAATCTTATTGCTATGGTTGAGCTTTTTATGAGGTTTGAAGCTTCTCAATATGAATACTCTAGTTCCAAGACTGTGCATCTAGCTGTTTTGCCGATGTTCCATTTATATGGTTTATCGCTCTTTGCTACCGGATTATTGTCATTGGGTTCTACAATCGTTGTAATGCGGAAATATGACATTGATGAGGCTATTAGGGCGATTGATAAATATAAAGTTACACACTTCCATGTTGTTCCAATGATGTTGTCGGCATTGACAGCGAAGGCAAAGGATGGTAATAGAAGTAAGTTGCAGAGTTTAAGACATGTCTCTTGCGGTGCTGAGCCTTTGAGCAAAGGAGCTATAAATGATTTTGTCCAAGCTTTTCCTAATGTTGATTTTATACAGGTAAAGAACCTTGGAGAAAAAAGAAAATTAGCCTTAATTAAAGAACGCAAGTCATGCTACATCATTTATATAATTGATTGAGTTATTTTTCTTTGTAATTGCTGTTAGGAGCTAAATAAATGCTGGTTTCATTTAGTTATGAGAGAACTTGTGAACTCTAAAATAATTGTTAATAGCAGATGCTAAACACTATTTCTTCGCCGTTATGGTATTCATATTCACAACATGTTCTGGACCATAATAGTTGAACTTTACTTGTTTATGGTAAAAATGTTGATTTCCTAGATTTTTAAGGATGTGAAGCTGTATGATTGTTTCTTTTTAGGGTTATGGAATGACTGAGTCGGGCGCAGTAGGAACACGCGGCTTCAATACTGATAAGTTTCATAACTATTCCTCACTAGGGTTGTTAGCTCCAAACATGGAGGCAAAAGTGGTAGATTGGAAAAGTGGTAAGTTTTTGCCCCCAGGCCTCAGCGGTGAGCTTTGGTTGAGAGGGCCTTCAATTATGAAAGGTAAAAAATCTGTATTTTTATAAGGTCTTGTCTATTGTGTGTAATCTTAACCTACATTTGCATGAGATTGATTCAATGATTTCAACTGCTGTGTTGCTATAACTTTTGTTTCTGTCTTATTCCTTAGCCTACTTGTACCTTTTTTTGCCTGTCTCTTTTGCTATTCCATTTGAAATTCTTAACCAAAATTTTTCCTTTATTTCATACTTCGTAGGATACTTGAATAATGAGGAAGCTACAATGTCAACAATTGATAAAGATGGTTGGCTACATACTGGAGATATTGTTTATTTTGATCAAGATGGATATTTATATTTGTCTGGCCGTTTGAAAGACATTATCAAATACAAGGGCTTTCAGGTATCAAACTTTTTGTTTATTAAACTTCTATAAGAAAATGTTCTTATACAATTTATTTTTACTAAATATGTATGCATTGTTCTTATGTATGGCACATGATCTTTTACAGATTGCTCCTGCTGATTTAGAAGCTTTGTTAATTTCGCATCCTGAAATAGTTGATGCCGCCGTTACAGCGTAAGTATATGAACACTTCATTTTGTCTTCTAAAGTGCCTCTTTGGACATTAAAATATGACCTTGAGTATCACATCATTTTCGATATCAGTGGCAAGGTTGATGAAGCTGGGGAGATTCCAGTGGCATTTGTGGTCAAGAAAGTCGGAAGTGTACTTTCTTCACAGCATGTGATCGATTACGTTGCTAAGCAGGTTTTTCTCTATGAGACAGACATGAAGTTCTAAGATATTTGAATCGATATGACTACTAGAGTCGATGTCATAGTGTCTATTACTATTGGAATGGTTTTCACATTTTAAACTTTTTTTTTTTTTCCTGGCAGGTTGCTCCATACAAGAAGGTTCGGAAAGTGGTCTTCACAGACAAGATACCGAGATCTGCAACTGGGAAAATCCTCCGACAGCAACTCAGGAATTGCTTGACTTCTAAGCTCTGA
Protein sequence

>MS.gene070122.t1

MATNWNFFSVPNDTIIYPHWYSTKTGIYSSLQPPRNLPTDPFLDVVSFIFSNPHDGVLALVDSTAGSSISYSNLLTLVKSLASGLHKMGVSQGDVVLLLLPNSIYYPIVLLSVMYLGAVVTPLNPLSSVSEIRKQVKECGVSFAFTIPENIKKLESLGIPVIAVPENEMDLKHDCFSCFYNLTYGKFYSIKKNLIYGDFDLPQRPVIKQEDTAGILYSSGTTGVSKGVVLTHRNLIAMVELFMRFEASQYEYSSSKTVHLAVLPMFHLYGLSLFATGLLSLGSTIVVMRKYDIDEAIRAIDKYKVTHFHVVPMMLSALTAKAKDGNRSKLQSLRHVSCGAEPLSKGAINDFVQAFPNVDFIQGYGMTESGAVGTRGFNTDKFHNYSSLGLLAPNMEAKVVDWKSGKFLPPGLSGELWLRGPSIMKGYLNNEEATMSTIDKDGWLHTGDIVYFDQDGYLYLSGRLKDIIKYKGFQIAPADLEALLISHPEIVDAAVTAGKVDEAGEIPVAFVVKKVGSVLSSQHVIDYVAKQVAPYKKVRKVVFTDKIPRSATGKILRQQLRNCLTSKL