AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80293


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80293.t1 MTR_6g059760 83.099 213 35 1 1 212 1 213 2.34e-126 355
MS.gene80293.t1 MTR_6g059755 84.496 129 19 1 85 212 2 130 1.85e-75 223
MS.gene80293.t1 MTR_6g059650 52.511 219 89 9 1 212 1 211 1.96e-54 173
MS.gene80293.t1 MTR_6g065460 45.933 209 100 8 1 201 1 204 2.67e-45 149
MS.gene80293.t1 MTR_6g044780 43.182 220 114 8 1 212 1 217 9.75e-43 144
MS.gene80293.t1 MTR_6g045097 43.182 220 114 8 1 212 1 217 2.08e-42 143
MS.gene80293.t1 MTR_6g044810 42.661 218 114 8 1 210 1 215 1.18e-41 140
MS.gene80293.t1 MTR_6g059410 44.601 213 99 9 1 207 1 200 1.05e-39 135
MS.gene80293.t1 MTR_6g059530 44.860 214 98 10 1 207 1 201 4.89e-39 133
MS.gene80293.t1 MTR_6g045403 41.364 220 117 9 1 212 1 216 6.25e-39 134
MS.gene80293.t1 MTR_6g045433 40.175 229 113 9 1 212 1 222 2.09e-36 127
MS.gene80293.t1 MTR_6g065460 38.647 207 94 7 1 201 1 180 2.26e-30 110
MS.gene80293.t1 MTR_3g463590 39.896 193 101 9 25 207 22 209 6.41e-30 110
MS.gene80293.t1 MTR_1g057110 37.931 203 103 9 1 199 1 184 6.68e-28 104
MS.gene80293.t1 MTR_6g066190 38.914 221 115 12 1 211 1 211 2.85e-27 103
MS.gene80293.t1 MTR_6g009650 38.679 212 111 11 1 202 1 203 3.27e-25 98.2
MS.gene80293.t1 MTR_6g059730 36.538 208 119 7 1 200 1 203 1.36e-24 96.7
MS.gene80293.t1 MTR_6g009563 37.089 213 114 12 1 202 1 204 1.21e-21 88.6
MS.gene80293.t1 MTR_6g066210 35.426 223 123 12 1 211 1 214 3.49e-21 87.8
MS.gene80293.t1 MTR_6g017225 34.722 216 118 11 1 201 1 208 5.14e-21 89.7
MS.gene80293.t1 MTR_6g059680 36.410 195 107 10 18 200 18 207 1.30e-20 86.3
MS.gene80293.t1 MTR_6g059810 36.150 213 103 14 6 198 9 208 5.35e-19 82.0
MS.gene80293.t1 MTR_1g035770 34.896 192 107 12 11 188 11 198 1.85e-14 69.7
MS.gene80293.t1 MTR_7g037410 32.558 172 99 8 29 189 27 192 1.18e-13 67.4
MS.gene80293.t1 MTR_6g078070 31.000 200 123 9 9 198 6 200 3.04e-12 63.5
MS.gene80293.t1 MTR_6g065570 44.156 77 37 3 15 85 11 87 4.66e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGTAAGAAATACTTTATT+TGG 0.169494 6.1:-59726284 MS.gene80293:CDS
GGTATTGGTGGTGGTGTTAA+TGG 0.238618 6.1:-59726018 MS.gene80293:CDS
CCTGGTTTATGTAATAATAT+TGG 0.276379 6.1:-59725907 MS.gene80293:CDS
TCCAAGTGGGTGGTAGTTAA+AGG 0.301512 6.1:-59726063 MS.gene80293:CDS
GGTAAGTAATGTAGGGTTCA+TGG 0.354211 6.1:+59726407 None:intergenic
TATTCAAACTACAGATCTTC+CGG 0.354335 6.1:+59725786 None:intergenic
ACAACTTGAGTAAAGGATAA+TGG 0.359989 6.1:+59726345 None:intergenic
TATTTCTTACTCACAAGAAC+GGG 0.363139 6.1:+59726294 None:intergenic
ATTGAACCATGGGTAGGTAT+TGG 0.417221 6.1:-59726033 MS.gene80293:CDS
TAGAGGGTGGTAAGTAATGT+AGG 0.427202 6.1:+59726399 None:intergenic
AATGGTAAGAGTATTATAGA+TGG 0.435603 6.1:-59726000 MS.gene80293:CDS
ACCTTTAACTACCACCCACT+TGG 0.461157 6.1:+59726062 None:intergenic
TCAATGAATACAACTTCAAA+GGG 0.470712 6.1:+59725829 None:intergenic
ATCAATGAATACAACTTCAA+AGG 0.473477 6.1:+59725828 None:intergenic
GGGCAAAGAGGAAGAAATAG+AGG 0.480997 6.1:+59726382 None:intergenic
TTGGGATAAATGTTAGTGGC+AGG 0.501980 6.1:+59726169 None:intergenic
ACCAGGTGTATCACTGGTGG+TGG 0.503174 6.1:+59725924 None:intergenic
TCTGTTGGGATAAATGTTAG+TGG 0.504722 6.1:+59726165 None:intergenic
TAATGGAAAGTAGGTGGCTA+GGG 0.507074 6.1:+59726362 None:intergenic
GTTGAAGATGCTGCTCGATC+CGG 0.510209 6.1:-59725805 MS.gene80293:CDS
TTTAACTACCACCCACTTGG+AGG 0.510750 6.1:+59726065 None:intergenic
AGTAAAGGATAATGGAAAGT+AGG 0.512041 6.1:+59726353 None:intergenic
CAGTCCACCACCACTTTCAC+CGG 0.513171 6.1:+59726257 None:intergenic
GAACCATGGGTAGGTATTGG+TGG 0.518436 6.1:-59726030 MS.gene80293:CDS
AGGTGGTTTCATTGAACCAT+GGG 0.524203 6.1:-59726043 MS.gene80293:CDS
TTGTTCAACAACTTGAGTAA+AGG 0.539696 6.1:+59726338 None:intergenic
CCACCACCACTTTCACCGGC+TGG 0.539880 6.1:+59726261 None:intergenic
AAATACTTTATTTGGCCAGC+CGG 0.543561 6.1:-59726276 MS.gene80293:CDS
ATTTGGCCAGCCGGTGAAAG+TGG 0.544335 6.1:-59726267 MS.gene80293:CDS
CCAGCCGGTGAAAGTGGTGG+TGG 0.546062 6.1:-59726261 MS.gene80293:CDS
ACATAAACCAGGTGTATCAC+TGG 0.553319 6.1:+59725918 None:intergenic
AAAGCTTGCTGTACAACAAG+TGG 0.555218 6.1:+59726210 None:intergenic
ATAATGGAAAGTAGGTGGCT+AGG 0.557559 6.1:+59726361 None:intergenic
CCATGGGTAGGTATTGGTGG+TGG 0.565594 6.1:-59726027 MS.gene80293:CDS
TCAAGTTGTTGAACAAGTGA+AGG 0.568787 6.1:-59726331 MS.gene80293:CDS
AAGGTGGTTTCATTGAACCA+TGG 0.574110 6.1:-59726044 MS.gene80293:CDS
GTATTTCTTACTCACAAGAA+CGG 0.581176 6.1:+59726293 None:intergenic
TTGTTTAAGATTGAGAGAAG+TGG 0.588206 6.1:-59725976 MS.gene80293:CDS
AGAGGGTGGTAAGTAATGTA+GGG 0.588469 6.1:+59726400 None:intergenic
CCAATATTATTACATAAACC+AGG 0.588965 6.1:+59725907 None:intergenic
CCCACCACCAGTGATACACC+TGG 0.602282 6.1:-59725925 MS.gene80293:CDS
GTGAAAGTGGTGGTGGACTG+AGG 0.603007 6.1:-59726254 MS.gene80293:CDS
AGTAGGTGGCTAGGGCAAAG+AGG 0.612636 6.1:+59726370 None:intergenic
GGTTTCATTGAACCATGGGT+AGG 0.618743 6.1:-59726039 MS.gene80293:CDS
AAGTGGGTGGTAGTTAAAGG+TGG 0.629514 6.1:-59726060 MS.gene80293:CDS
AAAGGATAATGGAAAGTAGG+TGG 0.636765 6.1:+59726356 None:intergenic
GGCAAAGAGGAAGAAATAGA+GGG 0.646580 6.1:+59726383 None:intergenic
AAAGAGGAAGAAATAGAGGG+TGG 0.652446 6.1:+59726386 None:intergenic
CCACCACCAATACCTACCCA+TGG 0.653181 6.1:+59726027 None:intergenic
TGGCCAGCCGGTGAAAGTGG+TGG 0.668540 6.1:-59726264 MS.gene80293:CDS
ATTTCTTACTCACAAGAACG+GGG 0.680212 6.1:+59726295 None:intergenic
TGCTGAATCCTCCAAGTGGG+TGG 0.681258 6.1:-59726073 MS.gene80293:CDS
CCAGGTGTATCACTGGTGGT+GGG 0.686983 6.1:+59725925 None:intergenic
TAAACCAGGTGTATCACTGG+TGG 0.706773 6.1:+59725921 None:intergenic
ATGTGCTGAATCCTCCAAGT+GGG 0.714019 6.1:-59726076 MS.gene80293:CDS
AATGTGCTGAATCCTCCAAG+TGG 0.759346 6.1:-59726077 MS.gene80293:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTTAGATAATGAAAA+TGG - chr6.1:59726311-59726330 MS.gene80293:CDS 15.0%
!! TGAGTAAGAAATACTTTATT+TGG - chr6.1:59725910-59725929 MS.gene80293:CDS 20.0%
!!! AAATCATTGATATTTTCTGT+TGG + chr6.1:59726047-59726066 None:intergenic 20.0%
!!! AATCATTGATATTTTCTGTT+GGG + chr6.1:59726046-59726065 None:intergenic 20.0%
! ATCAATGAATACAACTTCAA+AGG + chr6.1:59726369-59726388 None:intergenic 25.0%
! CCAATATTATTACATAAACC+AGG + chr6.1:59726290-59726309 None:intergenic 25.0%
! CCTGGTTTATGTAATAATAT+TGG - chr6.1:59726287-59726306 MS.gene80293:CDS 25.0%
! TCAATGAATACAACTTCAAA+GGG + chr6.1:59726368-59726387 None:intergenic 25.0%
!!! AATGGTAAGAGTATTATAGA+TGG - chr6.1:59726194-59726213 MS.gene80293:CDS 25.0%
ACAACTTGAGTAAAGGATAA+TGG + chr6.1:59725852-59725871 None:intergenic 30.0%
GAACAAGTGAAGGATAAAAA+TGG - chr6.1:59725873-59725892 MS.gene80293:CDS 30.0%
GTATTTCTTACTCACAAGAA+CGG + chr6.1:59725904-59725923 None:intergenic 30.0%
TATTTCTTACTCACAAGAAC+GGG + chr6.1:59725903-59725922 None:intergenic 30.0%
TTGTTCAACAACTTGAGTAA+AGG + chr6.1:59725859-59725878 None:intergenic 30.0%
! TTGTTTAAGATTGAGAGAAG+TGG - chr6.1:59726218-59726237 MS.gene80293:CDS 30.0%
!! AGTAAAGGATAATGGAAAGT+AGG + chr6.1:59725844-59725863 None:intergenic 30.0%
!!! ATTGAGAGAAGTGGTTTTTT+TGG - chr6.1:59726227-59726246 MS.gene80293:CDS 30.0%
ATTTCTTACTCACAAGAACG+GGG + chr6.1:59725902-59725921 None:intergenic 35.0%
TCTGTTGGGATAAATGTTAG+TGG + chr6.1:59726032-59726051 None:intergenic 35.0%
! AAATACTTTATTTGGCCAGC+CGG - chr6.1:59725918-59725937 MS.gene80293:CDS 35.0%
! TCAAGTTGTTGAACAAGTGA+AGG - chr6.1:59725863-59725882 MS.gene80293:CDS 35.0%
!! AAAGGATAATGGAAAGTAGG+TGG + chr6.1:59725841-59725860 None:intergenic 35.0%
!! TGTACAGCAAGCTTTTTCTA+AGG - chr6.1:59725992-59726011 MS.gene80293:CDS 35.0%
AAAGAGGAAGAAATAGAGGG+TGG + chr6.1:59725811-59725830 None:intergenic 40.0%
AAGGTGGTTTCATTGAACCA+TGG - chr6.1:59726150-59726169 MS.gene80293:CDS 40.0%
ACATAAACCAGGTGTATCAC+TGG + chr6.1:59726279-59726298 None:intergenic 40.0%
AGAGGGTGGTAAGTAATGTA+GGG + chr6.1:59725797-59725816 None:intergenic 40.0%
AGGTGGTTTCATTGAACCAT+GGG - chr6.1:59726151-59726170 MS.gene80293:CDS 40.0%
ATTGAACCATGGGTAGGTAT+TGG - chr6.1:59726161-59726180 MS.gene80293:CDS 40.0%
GGCAAAGAGGAAGAAATAGA+GGG + chr6.1:59725814-59725833 None:intergenic 40.0%
TAATGGAAAGTAGGTGGCTA+GGG + chr6.1:59725835-59725854 None:intergenic 40.0%
TAGAGGGTGGTAAGTAATGT+AGG + chr6.1:59725798-59725817 None:intergenic 40.0%
TTGGGATAAATGTTAGTGGC+AGG + chr6.1:59726028-59726047 None:intergenic 40.0%
! ATAATGGAAAGTAGGTGGCT+AGG + chr6.1:59725836-59725855 None:intergenic 40.0%
!! AAAGCTTGCTGTACAACAAG+TGG + chr6.1:59725987-59726006 None:intergenic 40.0%
AAGTGGGTGGTAGTTAAAGG+TGG - chr6.1:59726134-59726153 MS.gene80293:CDS 45.0%
AATGTGCTGAATCCTCCAAG+TGG - chr6.1:59726117-59726136 MS.gene80293:CDS 45.0%
ACCTTTAACTACCACCCACT+TGG + chr6.1:59726135-59726154 None:intergenic 45.0%
ATGTGCTGAATCCTCCAAGT+GGG - chr6.1:59726118-59726137 MS.gene80293:CDS 45.0%
GGGCAAAGAGGAAGAAATAG+AGG + chr6.1:59725815-59725834 None:intergenic 45.0%
GGTTTCATTGAACCATGGGT+AGG - chr6.1:59726155-59726174 MS.gene80293:CDS 45.0%
TAAACCAGGTGTATCACTGG+TGG + chr6.1:59726276-59726295 None:intergenic 45.0%
TCCAAGTGGGTGGTAGTTAA+AGG - chr6.1:59726131-59726150 MS.gene80293:CDS 45.0%
TTTAACTACCACCCACTTGG+AGG + chr6.1:59726132-59726151 None:intergenic 45.0%
!! GGTATTGGTGGTGGTGTTAA+TGG - chr6.1:59726176-59726195 MS.gene80293:CDS 45.0%
GTTGAAGATGCTGCTCGATC+CGG - chr6.1:59726389-59726408 MS.gene80293:CDS 50.0%
!! GAACCATGGGTAGGTATTGG+TGG - chr6.1:59726164-59726183 MS.gene80293:CDS 50.0%
ACCAGGTGTATCACTGGTGG+TGG + chr6.1:59726273-59726292 None:intergenic 55.0%
AGTAGGTGGCTAGGGCAAAG+AGG + chr6.1:59725827-59725846 None:intergenic 55.0%
ATTTGGCCAGCCGGTGAAAG+TGG - chr6.1:59725927-59725946 MS.gene80293:CDS 55.0%
CAGTCCACCACCACTTTCAC+CGG + chr6.1:59725940-59725959 None:intergenic 55.0%
CCACCACCAATACCTACCCA+TGG + chr6.1:59726170-59726189 None:intergenic 55.0%
CCAGGTGTATCACTGGTGGT+GGG + chr6.1:59726272-59726291 None:intergenic 55.0%
TGCTGAATCCTCCAAGTGGG+TGG - chr6.1:59726121-59726140 MS.gene80293:CDS 55.0%
!! CCATGGGTAGGTATTGGTGG+TGG - chr6.1:59726167-59726186 MS.gene80293:CDS 55.0%
!! GTGAAAGTGGTGGTGGACTG+AGG - chr6.1:59725940-59725959 MS.gene80293:CDS 55.0%
CCCACCACCAGTGATACACC+TGG - chr6.1:59726269-59726288 MS.gene80293:CDS 60.0%
CCACCACCACTTTCACCGGC+TGG + chr6.1:59725936-59725955 None:intergenic 65.0%
CCAGCCGGTGAAAGTGGTGG+TGG - chr6.1:59725933-59725952 MS.gene80293:CDS 65.0%
TGGCCAGCCGGTGAAAGTGG+TGG - chr6.1:59725930-59725949 MS.gene80293:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 59725789 59726427 59725789 ID=MS.gene80293
chr6.1 mRNA 59725789 59726427 59725789 ID=MS.gene80293.t1;Parent=MS.gene80293
chr6.1 exon 59725789 59726427 59725789 ID=MS.gene80293.t1.exon1;Parent=MS.gene80293.t1
chr6.1 CDS 59725789 59726427 59725789 ID=cds.MS.gene80293.t1;Parent=MS.gene80293.t1
Gene Sequence

>MS.gene80293

ATGAACCCTACATTACTTACCACCCTCTATTTCTTCCTCTTTGCCCTAGCCACCTACTTTCCATTATCCTTTACTCAAGTTGTTGAACAAGTGAAGGATAAAAATGGAAACCCCGTTCTTGTGAGTAAGAAATACTTTATTTGGCCAGCCGGTGAAAGTGGTGGTGGACTGAGGCTTAATGAGACAGAAGAATGTCCACTTGTTGTACAGCAAGCTTTTTCTAAGGATGTTAAAAGCCTGCCACTAACATTTATCCCAACAGAAAATATCAATGATTTCATCTTTACTCGCTTCTCATCACTAGATATTGTATTTGAAAAGAAGACAAAATGTGCTGAATCCTCCAAGTGGGTGGTAGTTAAAGGTGGTTTCATTGAACCATGGGTAGGTATTGGTGGTGGTGTTAATGGTAAGAGTATTATAGATGGTTTGTTTAAGATTGAGAGAAGTGGTTTTTTTGGATACAAGCTTATGTTTTGTCCCACCACCAGTGATACACCTGGTTTATGTAATAATATTGGAAGATTTTTAGATAATGAAAATGGAGTGCGTTTGATCATGAGTGAAAATTTTCAACCCTTTGAAGTTGTATTCATTGATGTTGAAGATGCTGCTCGATCCGGAAGATCTGTAGTTTGA

Protein sequence

>MS.gene80293.t1

MNPTLLTTLYFFLFALATYFPLSFTQVVEQVKDKNGNPVLVSKKYFIWPAGESGGGLRLNETEECPLVVQQAFSKDVKSLPLTFIPTENINDFIFTRFSSLDIVFEKKTKCAESSKWVVVKGGFIEPWVGIGGGVNGKSIIDGLFKIERSGFFGYKLMFCPTTSDTPGLCNNIGRFLDNENGVRLIMSENFQPFEVVFIDVEDAARSGRSVV