AlfalfaGEDB | MS.gene31542

M. sativa cultivar XinJiangDaYe / MS.gene31542

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31542.t1	XP_013464999.1	85.8	492	65	3	3	493	1	488	4.30E-242	847

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31542.t1	Q9ZWB4	41.0	476	250	14	19	485	9	462	4.6e-83	310.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31542.t1	A0A072VAG9	85.8	492	65	3	3	493	1	488	3.1e-242	847.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04925	MS.gene31542	0.822644	2.12E-53	-1.69E-46
MS.gene049549	MS.gene31542	0.810699	1.01E-50	-1.69E-46
MS.gene049813	MS.gene31542	0.842184	3.02E-58	-1.69E-46
MS.gene050344	MS.gene31542	0.810993	8.74E-51	-1.69E-46
MS.gene051127	MS.gene31542	0.810329	1.22E-50	-1.69E-46
MS.gene051973	MS.gene31542	0.836009	1.20E-56	-1.69E-46
MS.gene052211	MS.gene31542	0.826436	2.72E-54	-1.69E-46
MS.gene05240	MS.gene31542	0.81808	2.36E-52	-1.69E-46
MS.gene054277	MS.gene31542	0.819377	1.20E-52	-1.69E-46
MS.gene05470	MS.gene31542	-0.810055	1.39E-50	-1.69E-46
MS.gene054742	MS.gene31542	0.829373	5.34E-55	-1.69E-46
MS.gene055240	MS.gene31542	0.81203	5.20E-51	-1.69E-46
MS.gene055977	MS.gene31542	0.814833	1.26E-51	-1.69E-46
MS.gene056218	MS.gene31542	0.839957	1.16E-57	-1.69E-46
MS.gene056590	MS.gene31542	0.805495	1.30E-49	-1.69E-46
MS.gene056591	MS.gene31542	0.805259	1.46E-49	-1.69E-46
MS.gene056748	MS.gene31542	0.846227	2.48E-59	-1.69E-46
MS.gene056920	MS.gene31542	0.821993	3.00E-53	-1.69E-46
MS.gene056988	MS.gene31542	0.833145	6.31E-56	-1.69E-46
MS.gene057550	MS.gene31542	0.818315	2.09E-52	-1.69E-46
MS.gene057820	MS.gene31542	0.820204	7.76E-53	-1.69E-46
MS.gene058603	MS.gene31542	0.805059	1.60E-49	-1.69E-46
MS.gene059953	MS.gene31542	0.811089	8.34E-51	-1.69E-46
MS.gene059955	MS.gene31542	0.802643	5.09E-49	-1.69E-46
MS.gene060087	MS.gene31542	0.846706	1.84E-59	-1.69E-46
MS.gene060358	MS.gene31542	0.819191	1.32E-52	-1.69E-46
MS.gene061038	MS.gene31542	0.805909	1.06E-49	-1.69E-46
MS.gene061157	MS.gene31542	0.818984	1.47E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene31542	MS.gene021909	PPI
MS.gene31542	MS.gene91612	PPI
MS.gene31542	MS.gene021917	PPI
MS.gene31542	MS.gene021910	PPI
MS.gene31542	MS.gene44630	PPI
MS.gene31542	MS.gene069422	PPI
MS.gene31542	MS.gene44647	PPI
MS.gene31542	MS.gene021915	PPI
MS.gene31542	MS.gene44643	PPI
MS.gene91597	MS.gene31542	PPI
MS.gene31542	MS.gene48584	PPI
MS.gene91615	MS.gene31542	PPI
MS.gene31542	MS.gene021913	PPI
MS.gene31542	MS.gene069423	PPI
MS.gene31542	MS.gene91611	PPI
MS.gene31542	MS.gene91599	PPI
MS.gene31542	MS.gene021905	PPI
MS.gene31542	MS.gene91614	PPI
MS.gene31542	MS.gene91598	PPI
MS.gene31542	MS.gene021906	PPI
MS.gene31542	MS.gene44642	PPI
MS.gene44644	MS.gene31542	PPI
MS.gene31542	MS.gene021916	PPI
MS.gene31542	MS.gene48585	PPI
MS.gene48582	MS.gene31542	PPI
MS.gene31542	MS.gene91596	PPI
MS.gene31542	MS.gene021914	PPI
MS.gene31542	MS.gene48581	PPI
MS.gene31542	MS.gene91609	PPI
MS.gene31542	MS.gene44640	PPI
MS.gene31542	MS.gene021904	PPI
MS.gene31542	MS.gene91613	PPI
MS.gene31542	MS.gene44633	PPI
MS.gene31542	MS.gene91595	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31542.t1	MTR_2g089765	85.772	492	65	3	3	493	1	488	0.0	870
MS.gene31542.t1	MTR_2g089670	69.400	500	127	7	3	493	1	483	0.0	669
MS.gene31542.t1	MTR_8g012750	66.803	488	138	11	16	491	1	476	0.0	619
MS.gene31542.t1	MTR_2g089665	59.375	448	65	7	18	462	2	335	1.50e-169	482
MS.gene31542.t1	MTR_7g015860	42.589	479	243	9	18	487	5	460	1.06e-122	367
MS.gene31542.t1	MTR_3g028210	36.550	487	272	10	16	490	6	467	3.52e-101	311
MS.gene31542.t1	MTR_7g014330	39.583	480	245	15	19	483	3	452	2.33e-93	291
MS.gene31542.t1	MTR_2g089650	35.294	493	272	14	14	490	5	466	1.18e-89	282
MS.gene31542.t1	MTR_2g089755	35.546	467	259	13	41	490	37	478	1.96e-86	274
MS.gene31542.t1	MTR_7g014250	37.029	478	260	14	19	483	3	452	2.44e-83	265
MS.gene31542.t1	MTR_7g014230	37.395	476	261	14	19	483	5	454	7.08e-83	264
MS.gene31542.t1	MTR_6g015850	36.345	487	272	11	12	490	4	460	1.05e-82	264
MS.gene31542.t1	MTR_3g087640	35.294	493	267	17	19	488	10	473	2.29e-82	263
MS.gene31542.t1	MTR_2g089735	34.205	459	265	10	45	490	30	464	1.51e-81	261
MS.gene31542.t1	MTR_6g015830	37.143	490	267	14	12	490	4	463	4.68e-80	257
MS.gene31542.t1	MTR_6g015815	34.908	487	274	12	17	490	7	463	2.93e-77	249
MS.gene31542.t1	MTR_7g014200	33.069	505	282	15	4	489	3	470	1.72e-74	243
MS.gene31542.t1	MTR_6g015320	32.225	481	279	10	23	490	11	457	1.50e-72	237
MS.gene31542.t1	MTR_7g014160	32.577	485	294	12	17	487	8	473	1.05e-70	233
MS.gene31542.t1	MTR_6g015315	32.237	456	269	8	45	490	30	455	5.96e-69	228
MS.gene31542.t1	MTR_7g014610	32.136	501	289	15	15	492	5	477	1.10e-67	225
MS.gene31542.t1	MTR_7g013850	31.435	474	274	13	42	487	30	480	5.62e-64	215
MS.gene31542.t1	MTR_7g014620	32.258	465	258	12	37	487	11	432	2.36e-58	199
MS.gene31542.t1	MTR_7g014360	32.840	405	220	12	87	483	22	382	7.95e-51	178
MS.gene31542.t1	MTR_3g435300	35.507	276	152	9	19	284	4	263	2.39e-42	152
MS.gene31542.t1	MTR_2g089745	81.928	83	15	0	411	493	1	83	9.19e-37	130
MS.gene31542.t1	MTR_7g014310	30.460	348	168	11	125	469	29	305	3.13e-32	125
MS.gene31542.t1	MTR_6g015310	27.062	388	197	10	88	465	22	333	6.19e-32	125
MS.gene31542.t1	MTR_7g014630	37.333	225	121	7	15	234	6	215	1.07e-31	125
MS.gene31542.t1	MTR_7g014670	32.828	198	123	2	291	487	71	259	1.25e-28	114
MS.gene31542.t1	MTR_7g014180	38.764	178	95	5	18	191	7	174	5.08e-26	104
MS.gene31542.t1	MTR_8g041770	26.144	459	272	15	41	490	30	430	1.93e-21	97.1
MS.gene31542.t1	MTR_4g022720	23.810	420	274	11	58	472	41	419	8.76e-21	95.1
MS.gene31542.t1	MTR_4g094842	23.313	489	309	18	17	483	3	447	9.42e-18	85.9
MS.gene31542.t1	MTR_4g022670	24.769	432	274	15	17	439	3	392	1.76e-17	85.1
MS.gene31542.t1	MTR_4g022825	25.424	413	249	15	41	439	24	391	2.78e-17	84.3
MS.gene31542.t1	MTR_4g022800	23.219	379	255	11	67	439	45	393	6.37e-17	83.6
MS.gene31542.t1	MTR_6g045553	24.798	496	304	17	10	476	6	461	7.59e-17	83.2
MS.gene31542.t1	MTR_3g026310	23.394	436	297	11	41	469	24	429	1.67e-16	82.0
MS.gene31542.t1	MTR_4g022290	23.529	459	271	18	35	469	24	426	1.88e-16	82.0
MS.gene31542.t1	MTR_4g094828	22.986	422	258	14	37	439	25	398	2.38e-16	81.6
MS.gene31542.t1	MTR_3g070170	22.323	439	272	13	71	479	60	459	4.03e-16	81.3
MS.gene31542.t1	MTR_7g085930	23.017	517	310	19	17	495	4	470	4.17e-16	80.9
MS.gene31542.t1	MTR_7g014210	39.252	107	63	2	353	457	32	138	1.65e-15	74.7
MS.gene31542.t1	MTR_7g014205	36.441	118	55	3	343	455	18	120	3.75e-15	72.4
MS.gene31542.t1	MTR_7g014340	57.353	68	28	1	19	85	3	70	5.54e-15	71.2
MS.gene31542.t1	MTR_3g435290	34.711	121	76	2	368	488	1	118	6.25e-15	71.6
MS.gene31542.t1	MTR_2g105330	23.831	449	272	19	57	490	39	432	1.26e-14	76.3
MS.gene31542.t1	MTR_2g064495	23.462	439	280	19	57	485	39	431	1.59e-14	75.9
MS.gene31542.t1	MTR_3g098980	22.494	409	267	10	76	479	65	428	1.99e-14	75.5
MS.gene31542.t1	MTR_8g096530	24.766	428	254	17	76	491	58	429	3.08e-14	75.1
MS.gene31542.t1	MTR_7g014220	57.143	63	27	0	33	95	10	72	5.71e-14	67.8
MS.gene31542.t1	MTR_5g076700	24.599	374	226	13	74	438	62	388	1.01e-13	73.6
MS.gene31542.t1	MTR_7g114490	22.642	371	232	10	76	438	64	387	2.16e-13	72.8
MS.gene31542.t1	MTR_3g101900	23.884	448	286	17	41	476	30	434	2.87e-13	72.0
MS.gene31542.t1	MTR_7g114560	23.155	393	245	11	54	438	44	387	8.83e-13	70.5
MS.gene31542.t1	MTR_7g085990	24.319	514	298	22	18	492	1	462	1.27e-12	70.1
MS.gene31542.t1	MTR_3g101930	24.031	387	244	16	63	438	56	403	6.75e-12	67.8
MS.gene31542.t1	MTR_3g049150	25.796	314	177	13	150	439	118	399	2.67e-11	65.9
MS.gene31542.t1	MTR_3g026950	25.325	308	170	10	160	439	128	403	8.60e-11	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31542.t1	AT1G03940	40.373	483	257	14	19	492	9	469	7.59e-106	324
MS.gene31542.t1	AT1G03495	40.449	356	183	11	20	369	10	342	9.38e-72	232
MS.gene31542.t1	AT3G29670	31.328	482	288	13	16	487	1	449	7.15e-66	219
MS.gene31542.t1	AT5G61160	32.449	490	277	14	16	489	1	452	8.50e-66	219
MS.gene31542.t1	AT3G29635	31.893	486	291	12	16	490	1	457	5.97e-64	214
MS.gene31542.t1	AT3G29680	31.535	482	287	12	16	488	1	448	2.30e-60	205
MS.gene31542.t1	AT5G39090	32.143	476	282	14	17	485	4	445	2.22e-59	202
MS.gene31542.t1	AT3G29590	32.033	487	272	18	17	488	7	449	9.70e-59	201
MS.gene31542.t1	AT5G39050	29.719	498	302	13	10	489	2	469	9.35e-56	193
MS.gene31542.t1	AT5G39080	29.569	487	284	15	17	480	4	454	8.13e-54	188
MS.gene31542.t1	AT2G19070	23.937	447	283	13	59	485	40	449	2.68e-19	90.9
MS.gene31542.t1	AT3G29690	32.716	162	97	4	332	489	27	180	2.80e-16	77.4
MS.gene31542.t1	AT3G50270	24.601	439	264	17	20	439	7	397	1.44e-15	79.3
MS.gene31542.t1	AT3G48720	21.198	434	284	10	54	479	41	424	8.42e-15	77.0
MS.gene31542.t1	AT3G50300	25.114	438	263	17	18	439	4	392	1.06e-13	73.6
MS.gene31542.t1	AT5G41040	21.173	392	261	10	71	456	63	412	1.14e-13	73.6
MS.gene31542.t1	AT5G41040	21.320	394	258	11	71	456	79	428	1.45e-13	73.2
MS.gene31542.t1	AT3G29720	30.928	194	112	7	116	305	26	201	5.95e-13	68.6
MS.gene31542.t1	AT1G31490	22.398	442	259	16	58	472	43	427	3.34e-11	65.9

Find 104 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCATCATTTCATGAAATTT+TGG	0.059044	2.4:+11295944	MS.gene31542:CDS
GTCTCATAAACAGCCAATTT+TGG	0.236198	2.4:-11296645	None:intergenic
GCTGTTTATGAGACTGATTT+TGG	0.237069	2.4:+11296654	MS.gene31542:CDS
AATCGTTGATGCCTAATTAT+AGG	0.244855	2.4:+11296577	MS.gene31542:CDS
ATACGAGGTGGAGGTAAATT+TGG	0.246555	2.4:-11295805	None:intergenic
GGAGGAGTGTTATGGGAATT+TGG	0.249016	2.4:-11295667	None:intergenic
TTAGAGAATTTCCTATAATT+AGG	0.272716	2.4:-11296588	None:intergenic
TAACCCATTTCTTCAACTTC+TGG	0.280416	2.4:-11296209	None:intergenic
GTTGGTGATGGAACTAAATT+TGG	0.291404	2.4:-11295460	None:intergenic
GGTTGCTGGTTCACCAAAAT+TGG	0.296365	2.4:+11296632	MS.gene31542:CDS
TATCACCTTCATGCTGAATT+TGG	0.301546	2.4:-11295413	None:intergenic
TTTAAGAATGATGCTTTGTT+AGG	0.320839	2.4:+11296549	MS.gene31542:CDS
CATTCGTGTAATCCCTATAT+TGG	0.328930	2.4:+11295840	MS.gene31542:CDS
TTGTAACAAGTTCCTTGATT+TGG	0.331236	2.4:+11296280	MS.gene31542:CDS
GTGACTTGAATAGCCAATAT+AGG	0.343977	2.4:-11295853	None:intergenic
TTATAGGAAATTCTCTAAAC+CGG	0.347519	2.4:+11296593	MS.gene31542:CDS
ACGTCTCTTGTAGTGGTTGC+TGG	0.360866	2.4:+11296618	MS.gene31542:CDS
AGAAATGGAATCTGCGGGTT+TGG	0.362780	2.4:+11296128	MS.gene31542:CDS
TGAACTGCATCAGATTTCTT+AGG	0.374068	2.4:-11296687	None:intergenic
AAGTCCAGAAGTTGAAGAAA+TGG	0.376086	2.4:+11296205	MS.gene31542:CDS
ATTTCCCGTCTGCAGCAATA+TGG	0.378457	2.4:-11295918	None:intergenic
TAGAAGAAGATACGATGAAT+TGG	0.382944	2.4:-11295529	None:intergenic
TATACAAGTTTAAGACCCTT+TGG	0.383445	2.4:-11296075	None:intergenic
AAAGAAGTGTTGGAGGGTTA+AGG	0.387404	2.4:-11295617	None:intergenic
TCTCTTTCTGATTGCAGAGA+TGG	0.388820	2.4:+11296732	MS.gene31542:CDS
ATTCACAAGATGCTCAAAAC+TGG	0.405633	2.4:+11295770	MS.gene31542:CDS
GTATGTAAGGAGGAGTGTTA+TGG	0.407860	2.4:-11295675	None:intergenic
TGACTTGAATAGCCAATATA+GGG	0.419390	2.4:-11295852	None:intergenic
ATTTGGAGGGATGATGAGGT+TGG	0.424629	2.4:-11295650	None:intergenic
GGAAAGTGAGTGTTTGAGAA+TGG	0.424878	2.4:-11295596	None:intergenic
TATGTAAGGAGGAGTGTTAT+GGG	0.427366	2.4:-11295674	None:intergenic
GATGATGAGGTTGGAGGTGA+AGG	0.429029	2.4:-11295641	None:intergenic
GAATGGGAAGTGTTGTTTGT+AGG	0.432621	2.4:-11295579	None:intergenic
AGATTCTGTATCAAAAGAAA+TGG	0.440390	2.4:+11296113	MS.gene31542:CDS
TGGTATGTCGAAGAAAGTTA+AGG	0.445120	2.4:-11295491	None:intergenic
CAGATAGAGAAACCGGAGTT+CGG	0.445819	2.4:-11295883	None:intergenic
CTTTCTGATTGCAGAGATGG+TGG	0.446881	2.4:+11296735	MS.gene31542:CDS
GTAAAGAGAGATGAGTTAGT+TGG	0.455750	2.4:+11296474	MS.gene31542:CDS
GGTGGAGGTGGAATTGAGAT+TGG	0.460315	2.4:+11296753	MS.gene31542:CDS
GAAAGTGAGTGTTTGAGAAT+GGG	0.469129	2.4:-11295595	None:intergenic
AGGTGGAATTGAGATTGGTT+TGG	0.473934	2.4:+11296758	MS.gene31542:CDS
GTCACTGTTCTTCCGAACTC+CGG	0.487247	2.4:+11295871	MS.gene31542:CDS
GGTATGTCGAAGAAAGTTAA+GGG	0.489263	2.4:-11295490	None:intergenic
AGAAGAAGATACGATGAATT+GGG	0.491688	2.4:-11295528	None:intergenic
AGTCCAGAAGTTGAAGAAAT+GGG	0.492194	2.4:+11296206	MS.gene31542:CDS
GGGAATTTGGAGGGATGATG+AGG	0.494796	2.4:-11295654	None:intergenic
TCATCAAGGTAACGTATGTA+AGG	0.500527	2.4:-11295688	None:intergenic
TGAAGGGAAAGAAGTGTTGG+AGG	0.501627	2.4:-11295624	None:intergenic
ACTGAGGCAGATAGAGAAAC+CGG	0.503828	2.4:-11295890	None:intergenic
GGGATTGTTGTGGCTTCAAA+TGG	0.510486	2.4:+11296504	MS.gene31542:CDS
TGTAGCATGCGAATGAACTA+AGG	0.513925	2.4:+11296843	MS.gene31542:CDS
GTTTGTAGGAAGTGATGAGT+TGG	0.516421	2.4:-11295565	None:intergenic
AAGGGGAGAATGGTTGGTGA+TGG	0.521926	2.4:-11295472	None:intergenic
AGGTGAAGGGAAAGAAGTGT+TGG	0.523394	2.4:-11295627	None:intergenic
GGTGATGGAACTAAATTTGG+TGG	0.531792	2.4:-11295457	None:intergenic
ACTCTATTTCTTTCATGTGA+AGG	0.532029	2.4:-11296045	None:intergenic
AGATTGGTTTGGCTCTTGAG+AGG	0.535803	2.4:+11296769	MS.gene31542:CDS
GATCATACAAAACCAAATCA+AGG	0.536579	2.4:-11296292	None:intergenic
TTGTATGATCAAATCGGAGG+AGG	0.538608	2.4:+11296305	MS.gene31542:CDS
TGAGGATTCAGCGACGGTGA+AGG	0.543996	2.4:-11295722	None:intergenic
TGGAGGGATGATGAGGTTGG+AGG	0.545689	2.4:-11295647	None:intergenic
GGTGATAGCAAAAGTGATCA+AGG	0.548302	2.4:+11296327	MS.gene31542:CDS
AAAGTTAAGGGGAGAATGGT+TGG	0.550742	2.4:-11295478	None:intergenic
TCAAAAGAAATGGAATCTGC+GGG	0.555070	2.4:+11296123	MS.gene31542:CDS
GGAGGTAAATTTGGAACAAG+AGG	0.560790	2.4:-11295796	None:intergenic
TCGAGATTCTTATGTTAGCA+AGG	0.560871	2.4:+11296152	MS.gene31542:CDS
GAAGAAAGTTAAGGGGAGAA+TGG	0.561998	2.4:-11295482	None:intergenic
GATGAGTTGGATGAGGAAAG+TGG	0.563879	2.4:-11295552	None:intergenic
TACAACCATATTGCTGCAGA+CGG	0.567282	2.4:+11295913	MS.gene31542:CDS
TCTTGTTCTATACGAGGTGG+AGG	0.569390	2.4:-11295814	None:intergenic
ATCAAAAGAAATGGAATCTG+CGG	0.571655	2.4:+11296122	MS.gene31542:CDS
AAATAGAGTTAAAGATCCAA+AGG	0.571917	2.4:+11296059	MS.gene31542:CDS
ATGATGAGGTTGGAGGTGAA+GGG	0.572297	2.4:-11295640	None:intergenic
ACCGGGTACGTCTCTTGTAG+TGG	0.574375	2.4:+11296611	MS.gene31542:CDS
GATTGCAGAGATGGTGGAGG+TGG	0.575491	2.4:+11296741	MS.gene31542:CDS
AATAGAGTTAAAGATCCAAA+GGG	0.576796	2.4:+11296060	MS.gene31542:CDS
GCAGATTGTCGTGATAGCGT+TGG	0.581739	2.4:+11296396	MS.gene31542:CDS
AGGAAGTGATGAGTTGGATG+AGG	0.585446	2.4:-11295559	None:intergenic
GAAGGGAAAGAAGTGTTGGA+GGG	0.591041	2.4:-11295623	None:intergenic
TAACTTTCTTCGACATACCA+TGG	0.593348	2.4:+11295494	MS.gene31542:CDS
GAGTGTTATGGGAATTTGGA+GGG	0.596912	2.4:-11295663	None:intergenic
GGAGTGTTATGGGAATTTGG+AGG	0.601515	2.4:-11295664	None:intergenic
AGCAAAAGTGATCAAGGTGA+AGG	0.601720	2.4:+11296333	MS.gene31542:CDS
TCTGATTGCAGAGATGGTGG+AGG	0.603040	2.4:+11296738	MS.gene31542:CDS
ACAACCATATTGCTGCAGAC+GGG	0.604720	2.4:+11295914	MS.gene31542:CDS
TATAGGAAATTCTCTAAACC+GGG	0.606160	2.4:+11296594	MS.gene31542:CDS
TGTATGATCAAATCGGAGGA+GGG	0.608399	2.4:+11296306	MS.gene31542:CDS
ATCAGAAAGAGAAATCGAAA+CGG	0.609802	2.4:-11296721	None:intergenic
CGAGACCAAATTCAGCATGA+AGG	0.615814	2.4:+11295408	MS.gene31542:CDS
GTCTGCTGAGGATTCAGCGA+CGG	0.615979	2.4:-11295728	None:intergenic
TCATGTGAAGGAAGATCAAG+AGG	0.624288	2.4:-11296033	None:intergenic
TTATCTTGTTCTATACGAGG+TGG	0.627129	2.4:-11295817	None:intergenic
GATGGAACTAAATTTGGTGG+TGG	0.629084	2.4:-11295454	None:intergenic
CAGCAATATGGTTGTAACTG+AGG	0.631657	2.4:-11295906	None:intergenic
TTGTATTGCATCTAATGCTG+TGG	0.648941	2.4:+11296449	MS.gene31542:CDS
TCAAGGTAACGTATGTAAGG+AGG	0.651289	2.4:-11295685	None:intergenic
AGGAGATAGAGTCTTCATCA+AGG	0.656629	2.4:-11295702	None:intergenic
ACCACTACAAGAGACGTACC+CGG	0.658474	2.4:-11296612	None:intergenic
GAGAATGTTGAAGTCTGCTG+AGG	0.661878	2.4:-11295740	None:intergenic
GAGGTAAATTTGGAACAAGA+GGG	0.662819	2.4:-11295795	None:intergenic
ATGTTATCTTGTTCTATACG+AGG	0.669004	2.4:-11295820	None:intergenic
GTATGTCGAAGAAAGTTAAG+GGG	0.670105	2.4:-11295489	None:intergenic
TGTGAAGGAAGATCAAGAGG+CGG	0.686663	2.4:-11296030	None:intergenic
ATTGGGTCACAATAAAACCA+TGG	0.688914	2.4:-11295511	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAGAGAATTTCCTATAATT+AGG	-	chr2.4:11296591-11296610	None:intergenic	20.0%
!!!	CAATTTCCAAAATAAGTTTT+TGG	-	chr2.4:11296432-11296451	None:intergenic	20.0%
!!!	TCATCATTTCATGAAATTTT+GGG	+	chr2.4:11295945-11295964	MS.gene31542:CDS	20.0%
!!!	TCATTACCAAAAACTTATTT+TGG	+	chr2.4:11296423-11296442	MS.gene31542:CDS	20.0%
!!!	TTCATCATTTCATGAAATTT+TGG	+	chr2.4:11295944-11295963	MS.gene31542:CDS	20.0%
!!!	TTTGTTATTGTTTTTTGCTA+TGG	-	chr2.4:11295986-11296005	None:intergenic	20.0%
!	AAATAGAGTTAAAGATCCAA+AGG	+	chr2.4:11296059-11296078	MS.gene31542:CDS	25.0%
!	AATAGAGTTAAAGATCCAAA+GGG	+	chr2.4:11296060-11296079	MS.gene31542:CDS	25.0%
!	AGATTCTGTATCAAAAGAAA+TGG	+	chr2.4:11296113-11296132	MS.gene31542:CDS	25.0%
!	ATGAGTTAGTTGGAAAAAAT+GGG	+	chr2.4:11296484-11296503	MS.gene31542:CDS	25.0%
!	TTATAGGAAATTCTCTAAAC+CGG	+	chr2.4:11296593-11296612	MS.gene31542:CDS	25.0%
!!	TTTAAGAATGATGCTTTGTT+AGG	+	chr2.4:11296549-11296568	MS.gene31542:CDS	25.0%
!!!	TTGGTTTTGTATGATCAAAT+CGG	+	chr2.4:11296299-11296318	MS.gene31542:CDS	25.0%
	AATCGTTGATGCCTAATTAT+AGG	+	chr2.4:11296577-11296596	MS.gene31542:CDS	30.0%
	AGAAGAAGATACGATGAATT+GGG	-	chr2.4:11295531-11295550	None:intergenic	30.0%
	ATCAAAAGAAATGGAATCTG+CGG	+	chr2.4:11296122-11296141	MS.gene31542:CDS	30.0%
	ATCAGAAAGAGAAATCGAAA+CGG	-	chr2.4:11296724-11296743	None:intergenic	30.0%
	ATGTTATCTTGTTCTATACG+AGG	-	chr2.4:11295823-11295842	None:intergenic	30.0%
	GATCATACAAAACCAAATCA+AGG	-	chr2.4:11296295-11296314	None:intergenic	30.0%
	GATGAGTTAGTTGGAAAAAA+TGG	+	chr2.4:11296483-11296502	MS.gene31542:CDS	30.0%
	TAGAAGAAGATACGATGAAT+TGG	-	chr2.4:11295532-11295551	None:intergenic	30.0%
	TATAGGAAATTCTCTAAACC+GGG	+	chr2.4:11296594-11296613	MS.gene31542:CDS	30.0%
	TGACTTGAATAGCCAATATA+GGG	-	chr2.4:11295855-11295874	None:intergenic	30.0%
	TTGTAACAAGTTCCTTGATT+TGG	+	chr2.4:11296280-11296299	MS.gene31542:CDS	30.0%
!	ACTCTATTTCTTTCATGTGA+AGG	-	chr2.4:11296048-11296067	None:intergenic	30.0%
!	TATACAAGTTTAAGACCCTT+TGG	-	chr2.4:11296078-11296097	None:intergenic	30.0%
!!	CTGTTTATGAGACTGATTTT+GGG	+	chr2.4:11296655-11296674	MS.gene31542:CDS	30.0%
!!	TTTTGCTATGGCTTTTGAAA+GGG	-	chr2.4:11295974-11295993	None:intergenic	30.0%
!!	TTTTTGCTATGGCTTTTGAA+AGG	-	chr2.4:11295975-11295994	None:intergenic	30.0%
	AAGTCCAGAAGTTGAAGAAA+TGG	+	chr2.4:11296205-11296224	MS.gene31542:CDS	35.0%
	AGTCCAGAAGTTGAAGAAAT+GGG	+	chr2.4:11296206-11296225	MS.gene31542:CDS	35.0%
	ATTCACAAGATGCTCAAAAC+TGG	+	chr2.4:11295770-11295789	MS.gene31542:CDS	35.0%
	ATTGGGTCACAATAAAACCA+TGG	-	chr2.4:11295514-11295533	None:intergenic	35.0%
	CATTCGTGTAATCCCTATAT+TGG	+	chr2.4:11295840-11295859	MS.gene31542:CDS	35.0%
	GAAAGTGAGTGTTTGAGAAT+GGG	-	chr2.4:11295598-11295617	None:intergenic	35.0%
	GGTATGTCGAAGAAAGTTAA+GGG	-	chr2.4:11295493-11295512	None:intergenic	35.0%
	GTAAAGAGAGATGAGTTAGT+TGG	+	chr2.4:11296474-11296493	MS.gene31542:CDS	35.0%
	GTATGTCGAAGAAAGTTAAG+GGG	-	chr2.4:11295492-11295511	None:intergenic	35.0%
	GTGACTTGAATAGCCAATAT+AGG	-	chr2.4:11295856-11295875	None:intergenic	35.0%
	TAACCCATTTCTTCAACTTC+TGG	-	chr2.4:11296212-11296231	None:intergenic	35.0%
	TAACTTTCTTCGACATACCA+TGG	+	chr2.4:11295494-11295513	MS.gene31542:CDS	35.0%
	TATCACCTTCATGCTGAATT+TGG	-	chr2.4:11295416-11295435	None:intergenic	35.0%
	TCAAAAGAAATGGAATCTGC+GGG	+	chr2.4:11296123-11296142	MS.gene31542:CDS	35.0%
	TCATCAAGGTAACGTATGTA+AGG	-	chr2.4:11295691-11295710	None:intergenic	35.0%
	TCGAGATTCTTATGTTAGCA+AGG	+	chr2.4:11296152-11296171	MS.gene31542:CDS	35.0%
	TGAACTGCATCAGATTTCTT+AGG	-	chr2.4:11296690-11296709	None:intergenic	35.0%
	TGGAAAAAATGGGATTGTTG+TGG	+	chr2.4:11296494-11296513	MS.gene31542:CDS	35.0%
	TGGTATGTCGAAGAAAGTTA+AGG	-	chr2.4:11295494-11295513	None:intergenic	35.0%
	TTATCTTGTTCTATACGAGG+TGG	-	chr2.4:11295820-11295839	None:intergenic	35.0%
	TTGTATTGCATCTAATGCTG+TGG	+	chr2.4:11296449-11296468	MS.gene31542:CDS	35.0%
!	GAGGTAAATTTGGAACAAGA+GGG	-	chr2.4:11295798-11295817	None:intergenic	35.0%
!	GTCTCATAAACAGCCAATTT+TGG	-	chr2.4:11296648-11296667	None:intergenic	35.0%
!	GTTTTGTATGATCAAATCGG+AGG	+	chr2.4:11296302-11296321	MS.gene31542:CDS	35.0%
!!	GCTGTTTATGAGACTGATTT+TGG	+	chr2.4:11296654-11296673	MS.gene31542:CDS	35.0%
!!	GTTGGTGATGGAACTAAATT+TGG	-	chr2.4:11295463-11295482	None:intergenic	35.0%
!!	TATGTAAGGAGGAGTGTTAT+GGG	-	chr2.4:11295677-11295696	None:intergenic	35.0%
!!	TTATGAGACTGATTTTGGGT+GGG	+	chr2.4:11296659-11296678	MS.gene31542:CDS	35.0%
!!	TTTATGAGACTGATTTTGGG+TGG	+	chr2.4:11296658-11296677	MS.gene31542:CDS	35.0%
	AAAGTTAAGGGGAGAATGGT+TGG	-	chr2.4:11295481-11295500	None:intergenic	40.0%
	AGCAAAAGTGATCAAGGTGA+AGG	+	chr2.4:11296333-11296352	MS.gene31542:CDS	40.0%
	AGGAGATAGAGTCTTCATCA+AGG	-	chr2.4:11295705-11295724	None:intergenic	40.0%
	ATACGAGGTGGAGGTAAATT+TGG	-	chr2.4:11295808-11295827	None:intergenic	40.0%
	CAGCAATATGGTTGTAACTG+AGG	-	chr2.4:11295909-11295928	None:intergenic	40.0%
	GAAGAAAGTTAAGGGGAGAA+TGG	-	chr2.4:11295485-11295504	None:intergenic	40.0%
	GAGTGTTATGGGAATTTGGA+GGG	-	chr2.4:11295666-11295685	None:intergenic	40.0%
	GGAAAGTGAGTGTTTGAGAA+TGG	-	chr2.4:11295599-11295618	None:intergenic	40.0%
	GGTGATAGCAAAAGTGATCA+AGG	+	chr2.4:11296327-11296346	MS.gene31542:CDS	40.0%
	GTTTGTAGGAAGTGATGAGT+TGG	-	chr2.4:11295568-11295587	None:intergenic	40.0%
	TACAACCATATTGCTGCAGA+CGG	+	chr2.4:11295913-11295932	MS.gene31542:CDS	40.0%
	TCAAGGTAACGTATGTAAGG+AGG	-	chr2.4:11295688-11295707	None:intergenic	40.0%
	TCATGTGAAGGAAGATCAAG+AGG	-	chr2.4:11296036-11296055	None:intergenic	40.0%
	TCTCTTTCTGATTGCAGAGA+TGG	+	chr2.4:11296732-11296751	MS.gene31542:CDS	40.0%
	TGTATGATCAAATCGGAGGA+GGG	+	chr2.4:11296306-11296325	MS.gene31542:CDS	40.0%
	TTGTATGATCAAATCGGAGG+AGG	+	chr2.4:11296305-11296324	MS.gene31542:CDS	40.0%
!	AGGTGGAATTGAGATTGGTT+TGG	+	chr2.4:11296758-11296777	MS.gene31542:CDS	40.0%
!	GATGGAACTAAATTTGGTGG+TGG	-	chr2.4:11295457-11295476	None:intergenic	40.0%
!	GGAGGTAAATTTGGAACAAG+AGG	-	chr2.4:11295799-11295818	None:intergenic	40.0%
!!	AAAGAAGTGTTGGAGGGTTA+AGG	-	chr2.4:11295620-11295639	None:intergenic	40.0%
!!	GAATGGGAAGTGTTGTTTGT+AGG	-	chr2.4:11295582-11295601	None:intergenic	40.0%
!!	GGTGATGGAACTAAATTTGG+TGG	-	chr2.4:11295460-11295479	None:intergenic	40.0%
!!	GTATGTAAGGAGGAGTGTTA+TGG	-	chr2.4:11295678-11295697	None:intergenic	40.0%
!!	TATGAGACTGATTTTGGGTG+GGG	+	chr2.4:11296660-11296679	MS.gene31542:CDS	40.0%
	ACAACCATATTGCTGCAGAC+GGG	+	chr2.4:11295914-11295933	MS.gene31542:CDS	45.0%
	ACTGAGGCAGATAGAGAAAC+CGG	-	chr2.4:11295893-11295912	None:intergenic	45.0%
	AGAAATGGAATCTGCGGGTT+TGG	+	chr2.4:11296128-11296147	MS.gene31542:CDS	45.0%
	ATGATGAGGTTGGAGGTGAA+GGG	-	chr2.4:11295643-11295662	None:intergenic	45.0%
	ATTTCCCGTCTGCAGCAATA+TGG	-	chr2.4:11295921-11295940	None:intergenic	45.0%
	ATTTGGAGGGATGATGAGGT+TGG	-	chr2.4:11295653-11295672	None:intergenic	45.0%
	CAGATAGAGAAACCGGAGTT+CGG	-	chr2.4:11295886-11295905	None:intergenic	45.0%
	CGAGACCAAATTCAGCATGA+AGG	+	chr2.4:11295408-11295427	MS.gene31542:CDS	45.0%
	CTTTCTGATTGCAGAGATGG+TGG	+	chr2.4:11296735-11296754	MS.gene31542:CDS	45.0%
	GAGAATGTTGAAGTCTGCTG+AGG	-	chr2.4:11295743-11295762	None:intergenic	45.0%
	GGGATTGTTGTGGCTTCAAA+TGG	+	chr2.4:11296504-11296523	MS.gene31542:CDS	45.0%
	TCTTGTTCTATACGAGGTGG+AGG	-	chr2.4:11295817-11295836	None:intergenic	45.0%
	TGTGAAGGAAGATCAAGAGG+CGG	-	chr2.4:11296033-11296052	None:intergenic	45.0%
!	AGGAAGTGATGAGTTGGATG+AGG	-	chr2.4:11295562-11295581	None:intergenic	45.0%
!	AGGTGAAGGGAAAGAAGTGT+TGG	-	chr2.4:11295630-11295649	None:intergenic	45.0%
!	GATGAGTTGGATGAGGAAAG+TGG	-	chr2.4:11295555-11295574	None:intergenic	45.0%
!	GGAGTGTTATGGGAATTTGG+AGG	-	chr2.4:11295667-11295686	None:intergenic	45.0%
!!	AGATTGGTTTGGCTCTTGAG+AGG	+	chr2.4:11296769-11296788	MS.gene31542:CDS	45.0%
!!	ATGAGACTGATTTTGGGTGG+GGG	+	chr2.4:11296661-11296680	MS.gene31542:CDS	45.0%
!!	GAAGGGAAAGAAGTGTTGGA+GGG	-	chr2.4:11295626-11295645	None:intergenic	45.0%
!!	GGAGGAGTGTTATGGGAATT+TGG	-	chr2.4:11295670-11295689	None:intergenic	45.0%
!!	GGTTGCTGGTTCACCAAAAT+TGG	+	chr2.4:11296632-11296651	MS.gene31542:CDS	45.0%
!!	TGAAGGGAAAGAAGTGTTGG+AGG	-	chr2.4:11295627-11295646	None:intergenic	45.0%
!!	TGCTATGGCTTTTGAAAGGG+AGG	-	chr2.4:11295971-11295990	None:intergenic	45.0%
	ACCACTACAAGAGACGTACC+CGG	-	chr2.4:11296615-11296634	None:intergenic	50.0%
	ACGTCTCTTGTAGTGGTTGC+TGG	+	chr2.4:11296618-11296637	MS.gene31542:CDS	50.0%
	GATGATGAGGTTGGAGGTGA+AGG	-	chr2.4:11295644-11295663	None:intergenic	50.0%
	GGGAATTTGGAGGGATGATG+AGG	-	chr2.4:11295657-11295676	None:intergenic	50.0%
	GGTGGAGGTGGAATTGAGAT+TGG	+	chr2.4:11296753-11296772	MS.gene31542:CDS	50.0%
	GTCACTGTTCTTCCGAACTC+CGG	+	chr2.4:11295871-11295890	MS.gene31542:CDS	50.0%
!	GCAGATTGTCGTGATAGCGT+TGG	+	chr2.4:11296396-11296415	MS.gene31542:CDS	50.0%
!	TCTGATTGCAGAGATGGTGG+AGG	+	chr2.4:11296738-11296757	MS.gene31542:CDS	50.0%
!!	AAGGGGAGAATGGTTGGTGA+TGG	-	chr2.4:11295475-11295494	None:intergenic	50.0%
	ACCGGGTACGTCTCTTGTAG+TGG	+	chr2.4:11296611-11296630	MS.gene31542:CDS	55.0%
	GTCTGCTGAGGATTCAGCGA+CGG	-	chr2.4:11295731-11295750	None:intergenic	55.0%
	TGAGGATTCAGCGACGGTGA+AGG	-	chr2.4:11295725-11295744	None:intergenic	55.0%
	TGGAGGGATGATGAGGTTGG+AGG	-	chr2.4:11295650-11295669	None:intergenic	55.0%
!!	GATTGCAGAGATGGTGGAGG+TGG	+	chr2.4:11296741-11296760	MS.gene31542:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	11295373	11296863	11295373	ID=MS.gene31542
chr2.4	mRNA	11295373	11296863	11295373	ID=MS.gene31542.t1;Parent=MS.gene31542
chr2.4	exon	11295373	11296863	11295373	ID=MS.gene31542.t1.exon1;Parent=MS.gene31542.t1
chr2.4	CDS	11295373	11296863	11295373	ID=cds.MS.gene31542.t1;Parent=MS.gene31542.t1

Gene Sequence

>MS.gene31542

ATGAACATCACAAACACAAACACAAACATAACTCACGAGACCAAATTCAGCATGAAGGTGATAGAACATAGTCAAGTTGCACCACCACCAAATTTAGTTCCATCACCAACCATTCTCCCCTTAACTTTCTTCGACATACCATGGTTTTATTGTGACCCAATTCATCGTATCTTCTTCTACCACTTTCCTCATCCAACTCATCACTTCCTACAAACAACACTTCCCATTCTCAAACACTCACTTTCCTTAACCCTCCAACACTTCTTTCCCTTCACCTCCAACCTCATCATCCCTCCAAATTCCCATAACACTCCTCCTTACATACGTTACCTTGATGAAGACTCTATCTCCTTCACCGTCGCTGAATCCTCAGCAGACTTCAACATTCTCGTATCTGATTCACAAGATGCTCAAAACTGGCACCCTCTTGTTCCAAATTTACCTCCACCTCGTATAGAACAAGATAACATTCGTGTAATCCCTATATTGGCTATTCAAGTCACTGTTCTTCCGAACTCCGGTTTCTCTATCTGCCTCAGTTACAACCATATTGCTGCAGACGGGAAATCACTTCATCATTTCATGAAATTTTGGGCCTCCCTTTCAAAAGCCATAGCAAAAAACAATAACAAAAATAATTTGTCTCTTGAATACTCTCCGCCTCTTGATCTTCCTTCACATGAAAGAAATAGAGTTAAAGATCCAAAGGGTCTTAAACTTGTATACTTGCAACAATTAGAAGATTCTGTATCAAAAGAAATGGAATCTGCGGGTTTGGTTCGAGATTCTTATGTTAGCAAGGTACGAACAACTTTGATATTGAGCTATGAACAAGTCCAGAAGTTGAAGAAATGGGTTACTGATAAATGTAAAGATAGTGAAAGAATAAAACACTTGTCTACTTTTGTTGTAACAAGTTCCTTGATTTGGTTTTGTATGATCAAATCGGAGGAGGGTGATAGCAAAAGTGATCAAGGTGAAGGTGATTGTGTTGTTGATGATAACTGCTACTTTGTGATTCTTGCAGATTGTCGTGATAGCGTTGGATTTTCATTACCAAAAACTTATTTTGGAAATTGTATTGCATCTAATGCTGTGGCTGTAAAGAGAGATGAGTTAGTTGGAAAAAATGGGATTGTTGTGGCTTCAAATGGTATTGAAAAGAAGATAAGAGATTTTAAGAATGATGCTTTGTTAGGAGCTGAATCGTTGATGCCTAATTATAGGAAATTCTCTAAACCGGGTACGTCTCTTGTAGTGGTTGCTGGTTCACCAAAATTGGCTGTTTATGAGACTGATTTTGGGTGGGGGAAGCCTAAGAAATCTGATGCAGTTCATCTTGATTCGTCCGTTTCGATTTCTCTTTCTGATTGCAGAGATGGTGGAGGTGGAATTGAGATTGGTTTGGCTCTTGAGAGGTCTAGAATGACTAATTTCATATACATCTTTCAAGAACGACTTGATAACATTTGTAGCATGCGAATGAACTAA

Protein sequence

>MS.gene31542.t1

MNITNTNTNITHETKFSMKVIEHSQVAPPPNLVPSPTILPLTFFDIPWFYCDPIHRIFFYHFPHPTHHFLQTTLPILKHSLSLTLQHFFPFTSNLIIPPNSHNTPPYIRYLDEDSISFTVAESSADFNILVSDSQDAQNWHPLVPNLPPPRIEQDNIRVIPILAIQVTVLPNSGFSICLSYNHIAADGKSLHHFMKFWASLSKAIAKNNNKNNLSLEYSPPLDLPSHERNRVKDPKGLKLVYLQQLEDSVSKEMESAGLVRDSYVSKVRTTLILSYEQVQKLKKWVTDKCKDSERIKHLSTFVVTSSLIWFCMIKSEEGDSKSDQGEGDCVVDDNCYFVILADCRDSVGFSLPKTYFGNCIASNAVAVKRDELVGKNGIVVASNGIEKKIRDFKNDALLGAESLMPNYRKFSKPGTSLVVVAGSPKLAVYETDFGWGKPKKSDAVHLDSSVSISLSDCRDGGGGIEIGLALERSRMTNFIYIFQERLDNICSMRMN