AlfalfaGEDB | MS.gene35741

M. sativa cultivar XinJiangDaYe / MS.gene35741

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35741.t1	XP_013464999.1	86.4	477	60	3	1	476	16	488	7.60E-236	826.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35741.t1	Q9ZWB4	41.2	476	249	14	2	468	9	462	2.6e-83	310.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35741.t1	A0A072VAG9	86.4	477	60	3	1	476	16	488	5.5e-236	826.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04925	MS.gene35741	0.836366	9.76E-57	-1.69E-46
MS.gene04926	MS.gene35741	0.806237	9.07E-50	-1.69E-46
MS.gene049813	MS.gene35741	0.826544	2.56E-54	-1.69E-46
MS.gene050479	MS.gene35741	0.826004	3.44E-54	-1.69E-46
MS.gene050547	MS.gene35741	0.812335	4.46E-51	-1.69E-46
MS.gene050577	MS.gene35741	-0.804517	2.08E-49	-1.69E-46
MS.gene050720	MS.gene35741	0.800492	1.41E-48	-1.69E-46
MS.gene050883	MS.gene35741	0.802243	6.15E-49	-1.69E-46
MS.gene051127	MS.gene35741	0.807925	3.98E-50	-1.69E-46
MS.gene051973	MS.gene35741	0.831642	1.49E-55	-1.69E-46
MS.gene052164	MS.gene35741	-0.80121	1.00E-48	-1.69E-46
MS.gene052211	MS.gene35741	0.832918	7.19E-56	-1.69E-46
MS.gene05240	MS.gene35741	0.838485	2.80E-57	-1.69E-46
MS.gene052823	MS.gene35741	-0.806067	9.85E-50	-1.69E-46
MS.gene052825	MS.gene35741	-0.80014	1.66E-48	-1.69E-46
MS.gene053097	MS.gene35741	0.800922	1.15E-48	-1.69E-46
MS.gene053406	MS.gene35741	0.808821	2.57E-50	-1.69E-46
MS.gene053436	MS.gene35741	-0.802006	6.88E-49	-1.69E-46
MS.gene054277	MS.gene35741	0.834082	3.68E-56	-1.69E-46
MS.gene05470	MS.gene35741	-0.830292	3.19E-55	-1.69E-46
MS.gene054742	MS.gene35741	0.850831	1.32E-60	-1.69E-46
MS.gene055240	MS.gene35741	0.803171	3.96E-49	-1.69E-46
MS.gene055629	MS.gene35741	-0.816091	6.61E-52	-1.69E-46
MS.gene055977	MS.gene35741	0.83282	7.60E-56	-1.69E-46
MS.gene056076	MS.gene35741	0.805522	1.28E-49	-1.69E-46
MS.gene056218	MS.gene35741	0.817559	3.10E-52	-1.69E-46
MS.gene056590	MS.gene35741	0.820848	5.52E-53	-1.69E-46
MS.gene056591	MS.gene35741	0.821378	4.17E-53	-1.69E-46
MS.gene056680	MS.gene35741	0.819852	9.34E-53	-1.69E-46
MS.gene056748	MS.gene35741	0.831511	1.60E-55	-1.69E-46
MS.gene056920	MS.gene35741	0.84469	6.47E-59	-1.69E-46
MS.gene056988	MS.gene35741	0.82772	1.34E-54	-1.69E-46
MS.gene057261	MS.gene35741	0.815034	1.14E-51	-1.69E-46
MS.gene057550	MS.gene35741	0.81093	9.02E-51	-1.69E-46
MS.gene057820	MS.gene35741	0.840267	9.64E-58	-1.69E-46
MS.gene058544	MS.gene35741	-0.817759	2.79E-52	-1.69E-46
MS.gene058603	MS.gene35741	0.809154	2.18E-50	-1.69E-46
MS.gene059328	MS.gene35741	0.812711	3.70E-51	-1.69E-46
MS.gene059891	MS.gene35741	0.813976	1.95E-51	-1.69E-46
MS.gene059892	MS.gene35741	0.817541	3.13E-52	-1.69E-46
MS.gene059953	MS.gene35741	0.827817	1.27E-54	-1.69E-46
MS.gene059955	MS.gene35741	0.811636	6.34E-51	-1.69E-46
MS.gene060087	MS.gene35741	0.851136	1.08E-60	-1.69E-46
MS.gene060358	MS.gene35741	0.837363	5.43E-57	-1.69E-46
MS.gene061038	MS.gene35741	0.826887	2.12E-54	-1.69E-46
MS.gene061092	MS.gene35741	0.815383	9.51E-52	-1.69E-46
MS.gene061094	MS.gene35741	0.817615	3.01E-52	-1.69E-46
MS.gene061157	MS.gene35741	0.805489	1.30E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene35741	MS.gene069423	PPI
MS.gene35741	MS.gene91611	PPI
MS.gene35741	MS.gene91599	PPI
MS.gene35741	MS.gene44643	PPI
MS.gene35741	MS.gene021905	PPI
MS.gene35741	MS.gene91614	PPI
MS.gene35741	MS.gene91598	PPI
MS.gene35741	MS.gene48584	PPI
MS.gene35741	MS.gene44642	PPI
MS.gene35741	MS.gene021916	PPI
MS.gene35741	MS.gene021913	PPI
MS.gene35741	MS.gene91596	PPI
MS.gene35741	MS.gene021914	PPI
MS.gene35741	MS.gene91609	PPI
MS.gene35741	MS.gene021904	PPI
MS.gene91597	MS.gene35741	PPI
MS.gene35741	MS.gene91613	PPI
MS.gene35741	MS.gene44633	PPI
MS.gene35741	MS.gene021906	PPI
MS.gene35741	MS.gene021917	PPI
MS.gene35741	MS.gene48585	PPI
MS.gene35741	MS.gene44640	PPI
MS.gene35741	MS.gene48581	PPI
MS.gene91615	MS.gene35741	PPI
MS.gene48582	MS.gene35741	PPI
MS.gene35741	MS.gene44630	PPI
MS.gene35741	MS.gene069422	PPI
MS.gene35741	MS.gene91595	PPI
MS.gene35741	MS.gene44647	PPI
MS.gene35741	MS.gene021909	PPI
MS.gene35741	MS.gene021915	PPI
MS.gene35741	MS.gene91612	PPI
MS.gene44644	MS.gene35741	PPI
MS.gene35741	MS.gene021910	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35741.t1	MTR_2g089765	86.373	477	60	3	1	476	16	488	0.0	847
MS.gene35741.t1	MTR_2g089670	71.399	479	117	6	1	476	22	483	0.0	667
MS.gene35741.t1	MTR_8g012750	67.078	486	136	11	1	474	3	476	0.0	618
MS.gene35741.t1	MTR_2g089665	59.598	448	64	7	1	445	2	335	2.42e-170	483
MS.gene35741.t1	MTR_7g015860	43.006	479	241	9	1	470	5	460	4.18e-124	370
MS.gene35741.t1	MTR_3g028210	36.701	485	270	10	1	473	8	467	3.01e-102	314
MS.gene35741.t1	MTR_7g014330	38.958	480	248	15	2	466	3	452	8.55e-93	289
MS.gene35741.t1	MTR_2g089650	35.174	489	270	14	1	473	9	466	2.34e-89	281
MS.gene35741.t1	MTR_2g089755	35.332	467	260	13	24	473	37	478	1.36e-85	271
MS.gene35741.t1	MTR_7g014230	37.185	476	262	14	2	466	5	454	1.94e-83	265
MS.gene35741.t1	MTR_7g014250	36.820	478	261	14	2	466	3	452	2.55e-83	265
MS.gene35741.t1	MTR_6g015850	35.967	481	272	10	1	473	8	460	4.77e-82	261
MS.gene35741.t1	MTR_2g089735	34.205	459	265	10	28	473	30	464	6.40e-81	259
MS.gene35741.t1	MTR_3g087640	34.350	492	273	16	2	471	10	473	1.42e-80	258
MS.gene35741.t1	MTR_6g015830	37.500	464	254	12	18	473	28	463	4.67e-80	256
MS.gene35741.t1	MTR_6g015815	35.052	485	272	12	2	473	9	463	4.11e-78	251
MS.gene35741.t1	MTR_7g014200	34.012	491	276	14	1	472	9	470	5.78e-77	249
MS.gene35741.t1	MTR_6g015320	32.479	468	269	10	19	473	24	457	1.34e-72	237
MS.gene35741.t1	MTR_7g014160	32.851	484	292	12	1	470	9	473	5.67e-72	236
MS.gene35741.t1	MTR_7g014610	32.731	498	284	15	1	475	8	477	2.87e-70	231
MS.gene35741.t1	MTR_6g015315	32.237	456	269	8	28	473	30	455	1.47e-69	229
MS.gene35741.t1	MTR_7g013850	31.224	474	275	13	25	470	30	480	9.72e-64	214
MS.gene35741.t1	MTR_7g014620	32.688	465	256	12	20	470	11	432	5.79e-60	203
MS.gene35741.t1	MTR_7g014360	32.512	406	222	12	69	466	21	382	5.54e-51	178
MS.gene35741.t1	MTR_3g435300	35.507	276	152	9	2	267	4	263	7.74e-43	153
MS.gene35741.t1	MTR_2g089745	81.928	83	15	0	394	476	1	83	6.60e-37	130
MS.gene35741.t1	MTR_7g014310	30.460	348	168	11	108	452	29	305	3.03e-33	128
MS.gene35741.t1	MTR_6g015310	26.804	388	198	10	71	448	22	333	1.43e-31	124
MS.gene35741.t1	MTR_7g014630	37.387	222	119	7	1	217	9	215	1.52e-31	124
MS.gene35741.t1	MTR_7g014670	33.333	198	122	2	274	470	71	259	8.59e-30	117
MS.gene35741.t1	MTR_7g014180	39.326	178	94	5	1	174	7	174	1.10e-26	106
MS.gene35741.t1	MTR_8g041770	25.708	459	274	15	24	473	30	430	8.61e-21	95.1
MS.gene35741.t1	MTR_4g022720	23.341	437	289	11	24	455	24	419	1.30e-20	94.4
MS.gene35741.t1	MTR_4g022825	25.182	413	250	15	24	422	24	391	3.27e-17	84.3
MS.gene35741.t1	MTR_4g022670	25.121	414	262	14	18	422	18	392	3.43e-17	84.0
MS.gene35741.t1	MTR_3g070170	22.323	439	272	13	54	462	60	459	5.91e-17	83.6
MS.gene35741.t1	MTR_4g094842	22.951	488	310	18	1	466	4	447	6.15e-17	83.2
MS.gene35741.t1	MTR_4g022800	22.955	379	256	11	50	422	45	393	1.11e-16	82.4
MS.gene35741.t1	MTR_3g026310	23.288	438	299	11	22	452	22	429	1.15e-16	82.4
MS.gene35741.t1	MTR_7g014210	38.261	115	69	2	328	440	24	138	1.86e-16	77.4
MS.gene35741.t1	MTR_7g085930	22.868	516	310	19	1	478	5	470	3.25e-16	81.3
MS.gene35741.t1	MTR_4g094828	22.090	421	263	13	20	422	25	398	4.38e-16	80.9
MS.gene35741.t1	MTR_4g022290	23.312	459	272	18	18	452	24	426	5.65e-16	80.5
MS.gene35741.t1	MTR_3g435290	34.711	121	76	2	351	471	1	118	5.98e-15	71.6
MS.gene35741.t1	MTR_2g105330	23.608	449	273	19	40	473	39	432	6.60e-15	77.0
MS.gene35741.t1	MTR_2g064495	23.529	442	276	19	40	468	39	431	8.12e-15	76.6
MS.gene35741.t1	MTR_7g014340	57.353	68	28	1	2	68	3	70	1.35e-14	70.1
MS.gene35741.t1	MTR_3g098980	22.494	409	267	10	59	462	65	428	1.42e-14	75.9
MS.gene35741.t1	MTR_7g014205	35.593	118	56	3	326	438	18	120	4.86e-14	68.9
MS.gene35741.t1	MTR_5g076700	24.599	374	226	13	57	421	62	388	5.50e-14	74.3
MS.gene35741.t1	MTR_7g014220	57.143	63	27	0	16	78	10	72	5.77e-14	67.4
MS.gene35741.t1	MTR_8g096530	24.299	428	256	17	59	474	58	429	1.09e-13	73.2
MS.gene35741.t1	MTR_3g101900	23.661	448	287	17	24	459	30	434	1.57e-13	72.8
MS.gene35741.t1	MTR_7g114490	22.372	371	233	9	59	421	64	387	1.81e-13	72.8
MS.gene35741.t1	MTR_7g085990	24.125	514	299	22	1	475	1	462	6.69e-13	70.9
MS.gene35741.t1	MTR_7g114560	23.155	393	245	11	37	421	44	387	9.96e-13	70.5
MS.gene35741.t1	MTR_3g101930	23.773	387	245	15	46	421	56	403	1.33e-12	70.1
MS.gene35741.t1	MTR_1g040175	22.606	376	225	11	59	421	63	385	4.41e-12	68.6
MS.gene35741.t1	MTR_4g097090	23.500	400	250	10	40	421	45	406	2.41e-11	66.2
MS.gene35741.t1	MTR_3g049150	25.796	314	177	13	133	422	118	399	2.87e-11	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35741.t1	AT1G03940	40.580	483	256	14	2	475	9	469	3.56e-106	324
MS.gene35741.t1	AT1G03495	40.730	356	182	11	3	352	10	342	2.92e-72	233
MS.gene35741.t1	AT5G61160	33.129	489	271	15	1	472	3	452	1.47e-66	221
MS.gene35741.t1	AT3G29670	31.458	480	286	13	1	470	3	449	2.62e-66	220
MS.gene35741.t1	AT3G29635	31.818	484	290	12	1	473	3	457	1.01e-63	214
MS.gene35741.t1	AT3G29680	31.458	480	286	12	1	471	3	448	3.05e-60	204
MS.gene35741.t1	AT5G39090	32.000	475	282	14	1	468	5	445	2.79e-59	201
MS.gene35741.t1	AT3G29590	31.893	486	272	18	1	471	8	449	3.56e-58	199
MS.gene35741.t1	AT5G39050	29.796	490	296	13	1	472	10	469	8.87e-55	190
MS.gene35741.t1	AT5G39080	29.012	486	286	14	1	463	5	454	2.47e-53	186
MS.gene35741.t1	AT2G19070	23.937	447	283	13	42	468	40	449	7.41e-20	92.4
MS.gene35741.t1	AT3G29690	32.716	162	97	4	315	472	27	180	6.87e-17	79.0
MS.gene35741.t1	AT3G50270	24.601	439	264	17	3	422	7	397	2.69e-16	81.6
MS.gene35741.t1	AT3G48720	21.198	434	284	10	37	462	41	424	6.21e-15	77.0
MS.gene35741.t1	AT5G41040	21.429	392	260	10	54	439	63	412	1.17e-14	76.3
MS.gene35741.t1	AT5G41040	21.574	394	257	11	54	439	79	428	2.10e-14	75.5
MS.gene35741.t1	AT3G50300	24.711	433	260	17	7	422	9	392	2.49e-13	72.4
MS.gene35741.t1	AT3G29720	30.412	194	113	7	99	288	26	201	9.61e-13	67.8
MS.gene35741.t1	AT1G31490	22.624	442	258	16	41	455	43	427	2.40e-12	69.3

Find 101 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCATCATTTCATGAAATTT+TGG	0.059044	2.1:+12426946	MS.gene35741:CDS
GTCTCATAAACAGCCAATTT+TGG	0.236198	2.1:-12427647	None:intergenic
GCTGTTTATGAGACTGATTT+TGG	0.237069	2.1:+12427656	MS.gene35741:CDS
AATCGTTGATGCCTAATTAT+AGG	0.244855	2.1:+12427579	MS.gene35741:CDS
ATACGAGGTGGAGGTAAATT+TGG	0.246555	2.1:-12426807	None:intergenic
GGAGGAGTGTTATGGGAATT+TGG	0.249016	2.1:-12426669	None:intergenic
TTAGAGAATTTCCTATAATT+AGG	0.272716	2.1:-12427590	None:intergenic
TAACCCATTTCTTCAACTTC+TGG	0.280416	2.1:-12427211	None:intergenic
GTTGGTGATGGAACTAAATT+TGG	0.291404	2.1:-12426462	None:intergenic
GGTTGCTGGTTCACCAAAAT+TGG	0.296365	2.1:+12427634	MS.gene35741:CDS
GTGACTTGAATAGCCATTAT+AGG	0.302545	2.1:-12426855	None:intergenic
TTTAAGAATGATGCTTTGTT+AGG	0.320839	2.1:+12427551	MS.gene35741:CDS
TTGTAACAAGTTCCTTGATT+TGG	0.331236	2.1:+12427282	MS.gene35741:CDS
TTATAGGAAATTCTCTAAAC+CGG	0.347519	2.1:+12427595	MS.gene35741:CDS
CATTCGTGTAATCCCTATAA+TGG	0.357080	2.1:+12426842	MS.gene35741:CDS
TGACTTGAATAGCCATTATA+GGG	0.360665	2.1:-12426854	None:intergenic
ACGTCTCTTGTAGTGGTTGC+TGG	0.360866	2.1:+12427620	MS.gene35741:CDS
AGAAATGGAATCTGCGGGTT+TGG	0.362780	2.1:+12427130	MS.gene35741:CDS
TGAACTGCATCAGATTTCTT+AGG	0.374068	2.1:-12427689	None:intergenic
AAGTCCAGAAGTTGAAGAAA+TGG	0.376086	2.1:+12427207	MS.gene35741:CDS
ATTTCCCGTCTGCAGCAATA+TGG	0.378457	2.1:-12426920	None:intergenic
TAGAAGAAGATACGATGAAT+TGG	0.382944	2.1:-12426531	None:intergenic
TATACAAGTTTAAGACCCTT+TGG	0.383445	2.1:-12427077	None:intergenic
AAAGAAGTGTTGGAGGGTTA+AGG	0.387404	2.1:-12426619	None:intergenic
TCTCTTTCTGATTGCAGAGA+TGG	0.388820	2.1:+12427734	MS.gene35741:CDS
ATTCACAAGATGCTCAAAAC+TGG	0.405633	2.1:+12426772	MS.gene35741:CDS
GTATGTAAGGAGGAGTGTTA+TGG	0.407860	2.1:-12426677	None:intergenic
ATTTGGAGGGATGATGAGGT+TGG	0.424629	2.1:-12426652	None:intergenic
GGAAAGTGAGTGTTTGAGAA+TGG	0.424878	2.1:-12426598	None:intergenic
TATGTAAGGAGGAGTGTTAT+GGG	0.427366	2.1:-12426676	None:intergenic
GATGATGAGGTTGGAGGTGA+AGG	0.429029	2.1:-12426643	None:intergenic
GAATGGGAAGTGTTGTTTGT+AGG	0.432621	2.1:-12426581	None:intergenic
AGATTCTGTATCAAAAGAAA+TGG	0.440390	2.1:+12427115	MS.gene35741:CDS
TGGTATGTCGAAGAAAGTTA+AGG	0.445120	2.1:-12426493	None:intergenic
CAGATAGAGAAACCGGAGTT+CGG	0.445819	2.1:-12426885	None:intergenic
CTTTCTGATTGCAGAGATGG+TGG	0.446881	2.1:+12427737	MS.gene35741:CDS
GTAAAGAGAGATGAGTTAGT+TGG	0.455750	2.1:+12427476	MS.gene35741:CDS
GGTGGAGGTGGAATTGAGAT+TGG	0.460315	2.1:+12427755	MS.gene35741:CDS
GAAAGTGAGTGTTTGAGAAT+GGG	0.469129	2.1:-12426597	None:intergenic
AGGTGGAATTGAGATTGGTT+TGG	0.473934	2.1:+12427760	MS.gene35741:CDS
GTCACTGTTCTTCCGAACTC+CGG	0.487247	2.1:+12426873	MS.gene35741:CDS
GGTATGTCGAAGAAAGTTAA+GGG	0.489263	2.1:-12426492	None:intergenic
AGAAGAAGATACGATGAATT+GGG	0.491688	2.1:-12426530	None:intergenic
AGTCCAGAAGTTGAAGAAAT+GGG	0.492194	2.1:+12427208	MS.gene35741:CDS
GGGAATTTGGAGGGATGATG+AGG	0.494796	2.1:-12426656	None:intergenic
TCATCAAGGTAACGTATGTA+AGG	0.500527	2.1:-12426690	None:intergenic
TGAAGGGAAAGAAGTGTTGG+AGG	0.501627	2.1:-12426626	None:intergenic
ACTGAGGCAGATAGAGAAAC+CGG	0.503828	2.1:-12426892	None:intergenic
GGGATTGTTGTGGCTTCAAA+TGG	0.510486	2.1:+12427506	MS.gene35741:CDS
TGTAGCATGCGAATGAACTA+AGG	0.513925	2.1:+12427845	MS.gene35741:CDS
GTTTGTAGGAAGTGATGAGT+TGG	0.516421	2.1:-12426567	None:intergenic
AAGGGGAGAATGGTTGGTGA+TGG	0.521926	2.1:-12426474	None:intergenic
AGGTGAAGGGAAAGAAGTGT+TGG	0.523394	2.1:-12426629	None:intergenic
GGTGATGGAACTAAATTTGG+TGG	0.531792	2.1:-12426459	None:intergenic
ACTCTATTTCTTTCATGTGA+AGG	0.532029	2.1:-12427047	None:intergenic
AGATTGGTTTGGCTCTTGAG+AGG	0.535803	2.1:+12427771	MS.gene35741:CDS
GATCATACAAAACCAAATCA+AGG	0.536579	2.1:-12427294	None:intergenic
TTGTATGATCAAATCGGAGG+AGG	0.538608	2.1:+12427307	MS.gene35741:CDS
TGAGGATTCAGCGACGGTGA+AGG	0.543996	2.1:-12426724	None:intergenic
TGGAGGGATGATGAGGTTGG+AGG	0.545689	2.1:-12426649	None:intergenic
GGTGATAGCAAAAGTGATCA+AGG	0.548302	2.1:+12427329	MS.gene35741:CDS
AAAGTTAAGGGGAGAATGGT+TGG	0.550742	2.1:-12426480	None:intergenic
TCAAAAGAAATGGAATCTGC+GGG	0.555070	2.1:+12427125	MS.gene35741:CDS
GGAGGTAAATTTGGAACAAG+AGG	0.560790	2.1:-12426798	None:intergenic
TCGAGATTCTTATGTTAGCA+AGG	0.560871	2.1:+12427154	MS.gene35741:CDS
GAAGAAAGTTAAGGGGAGAA+TGG	0.561998	2.1:-12426484	None:intergenic
GATGAGTTGGATGAGGAAAG+TGG	0.563879	2.1:-12426554	None:intergenic
TACAACCATATTGCTGCAGA+CGG	0.567282	2.1:+12426915	MS.gene35741:CDS
TCTTGTTCTATACGAGGTGG+AGG	0.569390	2.1:-12426816	None:intergenic
ATCAAAAGAAATGGAATCTG+CGG	0.571655	2.1:+12427124	MS.gene35741:CDS
AAATAGAGTTAAAGATCCAA+AGG	0.571917	2.1:+12427061	MS.gene35741:CDS
ATGATGAGGTTGGAGGTGAA+GGG	0.572297	2.1:-12426642	None:intergenic
ACCGGGTACGTCTCTTGTAG+TGG	0.574375	2.1:+12427613	MS.gene35741:CDS
GATTGCAGAGATGGTGGAGG+TGG	0.575491	2.1:+12427743	MS.gene35741:CDS
AATAGAGTTAAAGATCCAAA+GGG	0.576796	2.1:+12427062	MS.gene35741:CDS
AGGAAGTGATGAGTTGGATG+AGG	0.585446	2.1:-12426561	None:intergenic
GAAGGGAAAGAAGTGTTGGA+GGG	0.591041	2.1:-12426625	None:intergenic
TAACTTTCTTCGACATACCA+TGG	0.593348	2.1:+12426496	MS.gene35741:CDS
GAGTGTTATGGGAATTTGGA+GGG	0.596912	2.1:-12426665	None:intergenic
GGAGTGTTATGGGAATTTGG+AGG	0.601515	2.1:-12426666	None:intergenic
AGCAAAAGTGATCAAGGTGA+AGG	0.601720	2.1:+12427335	MS.gene35741:CDS
TCTGATTGCAGAGATGGTGG+AGG	0.603040	2.1:+12427740	MS.gene35741:CDS
ACAACCATATTGCTGCAGAC+GGG	0.604720	2.1:+12426916	MS.gene35741:CDS
TATAGGAAATTCTCTAAACC+GGG	0.606160	2.1:+12427596	MS.gene35741:CDS
TGTATGATCAAATCGGAGGA+GGG	0.608399	2.1:+12427308	MS.gene35741:CDS
ATCAGAAAGAGAAATCGAAA+CGG	0.609802	2.1:-12427723	None:intergenic
GTCTGCTGAGGATTCAGCGA+CGG	0.615979	2.1:-12426730	None:intergenic
TCATGTGAAGGAAGATCAAG+AGG	0.624288	2.1:-12427035	None:intergenic
TTATCTTGTTCTATACGAGG+TGG	0.627129	2.1:-12426819	None:intergenic
CAGCAATATGGTTGTAACTG+AGG	0.631657	2.1:-12426908	None:intergenic
TTGTATTGCATCTAATGCTG+TGG	0.648941	2.1:+12427451	MS.gene35741:CDS
TCAAGGTAACGTATGTAAGG+AGG	0.651289	2.1:-12426687	None:intergenic
AGGAGATAGAGTCTTCATCA+AGG	0.656629	2.1:-12426704	None:intergenic
ACCACTACAAGAGACGTACC+CGG	0.658474	2.1:-12427614	None:intergenic
GAGAATGTTGAAGTCTGCTG+AGG	0.661878	2.1:-12426742	None:intergenic
GAGGTAAATTTGGAACAAGA+GGG	0.662819	2.1:-12426797	None:intergenic
GATGGAACTAAATTTGGTGG+TGG	0.666610	2.1:-12426456	None:intergenic
ATGTTATCTTGTTCTATACG+AGG	0.669004	2.1:-12426822	None:intergenic
GTATGTCGAAGAAAGTTAAG+GGG	0.670105	2.1:-12426491	None:intergenic
TGTGAAGGAAGATCAAGAGG+CGG	0.686663	2.1:-12427032	None:intergenic
ATTGGGTCACAATAAAACCA+TGG	0.688914	2.1:-12426513	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAGAGAATTTCCTATAATT+AGG	-	chr2.1:12427593-12427612	None:intergenic	20.0%
!!!	CAATTTCCAAAATAAGTTTT+TGG	-	chr2.1:12427434-12427453	None:intergenic	20.0%
!!!	TCATCATTTCATGAAATTTT+GGG	+	chr2.1:12426947-12426966	MS.gene35741:CDS	20.0%
!!!	TCATTACCAAAAACTTATTT+TGG	+	chr2.1:12427425-12427444	MS.gene35741:CDS	20.0%
!!!	TTCATCATTTCATGAAATTT+TGG	+	chr2.1:12426946-12426965	MS.gene35741:CDS	20.0%
!!!	TTTGTTATTGTTTTTTGCTA+TGG	-	chr2.1:12426988-12427007	None:intergenic	20.0%
!	AAATAGAGTTAAAGATCCAA+AGG	+	chr2.1:12427061-12427080	MS.gene35741:CDS	25.0%
!	AATAGAGTTAAAGATCCAAA+GGG	+	chr2.1:12427062-12427081	MS.gene35741:CDS	25.0%
!	AGATTCTGTATCAAAAGAAA+TGG	+	chr2.1:12427115-12427134	MS.gene35741:CDS	25.0%
!	ATGAGTTAGTTGGAAAAAAT+GGG	+	chr2.1:12427486-12427505	MS.gene35741:CDS	25.0%
!	TTATAGGAAATTCTCTAAAC+CGG	+	chr2.1:12427595-12427614	MS.gene35741:CDS	25.0%
!!	TTTAAGAATGATGCTTTGTT+AGG	+	chr2.1:12427551-12427570	MS.gene35741:CDS	25.0%
!!!	TTGGTTTTGTATGATCAAAT+CGG	+	chr2.1:12427301-12427320	MS.gene35741:CDS	25.0%
	AATCGTTGATGCCTAATTAT+AGG	+	chr2.1:12427579-12427598	MS.gene35741:CDS	30.0%
	AGAAGAAGATACGATGAATT+GGG	-	chr2.1:12426533-12426552	None:intergenic	30.0%
	ATCAAAAGAAATGGAATCTG+CGG	+	chr2.1:12427124-12427143	MS.gene35741:CDS	30.0%
	ATCAGAAAGAGAAATCGAAA+CGG	-	chr2.1:12427726-12427745	None:intergenic	30.0%
	ATGTTATCTTGTTCTATACG+AGG	-	chr2.1:12426825-12426844	None:intergenic	30.0%
	GATCATACAAAACCAAATCA+AGG	-	chr2.1:12427297-12427316	None:intergenic	30.0%
	GATGAGTTAGTTGGAAAAAA+TGG	+	chr2.1:12427485-12427504	MS.gene35741:CDS	30.0%
	TAGAAGAAGATACGATGAAT+TGG	-	chr2.1:12426534-12426553	None:intergenic	30.0%
	TATAGGAAATTCTCTAAACC+GGG	+	chr2.1:12427596-12427615	MS.gene35741:CDS	30.0%
	TGACTTGAATAGCCATTATA+GGG	-	chr2.1:12426857-12426876	None:intergenic	30.0%
	TTGTAACAAGTTCCTTGATT+TGG	+	chr2.1:12427282-12427301	MS.gene35741:CDS	30.0%
!	ACTCTATTTCTTTCATGTGA+AGG	-	chr2.1:12427050-12427069	None:intergenic	30.0%
!	TATACAAGTTTAAGACCCTT+TGG	-	chr2.1:12427080-12427099	None:intergenic	30.0%
!!	CTGTTTATGAGACTGATTTT+GGG	+	chr2.1:12427657-12427676	MS.gene35741:CDS	30.0%
!!	TTTTGCTATGGCTTTTGAAA+GGG	-	chr2.1:12426976-12426995	None:intergenic	30.0%
!!	TTTTTGCTATGGCTTTTGAA+AGG	-	chr2.1:12426977-12426996	None:intergenic	30.0%
	AAGTCCAGAAGTTGAAGAAA+TGG	+	chr2.1:12427207-12427226	MS.gene35741:CDS	35.0%
	AGTCCAGAAGTTGAAGAAAT+GGG	+	chr2.1:12427208-12427227	MS.gene35741:CDS	35.0%
	ATTCACAAGATGCTCAAAAC+TGG	+	chr2.1:12426772-12426791	MS.gene35741:CDS	35.0%
	ATTGGGTCACAATAAAACCA+TGG	-	chr2.1:12426516-12426535	None:intergenic	35.0%
	CATTCGTGTAATCCCTATAA+TGG	+	chr2.1:12426842-12426861	MS.gene35741:CDS	35.0%
	GAAAGTGAGTGTTTGAGAAT+GGG	-	chr2.1:12426600-12426619	None:intergenic	35.0%
	GGTATGTCGAAGAAAGTTAA+GGG	-	chr2.1:12426495-12426514	None:intergenic	35.0%
	GTAAAGAGAGATGAGTTAGT+TGG	+	chr2.1:12427476-12427495	MS.gene35741:CDS	35.0%
	GTATGTCGAAGAAAGTTAAG+GGG	-	chr2.1:12426494-12426513	None:intergenic	35.0%
	GTGACTTGAATAGCCATTAT+AGG	-	chr2.1:12426858-12426877	None:intergenic	35.0%
	TAACCCATTTCTTCAACTTC+TGG	-	chr2.1:12427214-12427233	None:intergenic	35.0%
	TAACTTTCTTCGACATACCA+TGG	+	chr2.1:12426496-12426515	MS.gene35741:CDS	35.0%
	TCAAAAGAAATGGAATCTGC+GGG	+	chr2.1:12427125-12427144	MS.gene35741:CDS	35.0%
	TCATCAAGGTAACGTATGTA+AGG	-	chr2.1:12426693-12426712	None:intergenic	35.0%
	TCGAGATTCTTATGTTAGCA+AGG	+	chr2.1:12427154-12427173	MS.gene35741:CDS	35.0%
	TGAACTGCATCAGATTTCTT+AGG	-	chr2.1:12427692-12427711	None:intergenic	35.0%
	TGGAAAAAATGGGATTGTTG+TGG	+	chr2.1:12427496-12427515	MS.gene35741:CDS	35.0%
	TGGTATGTCGAAGAAAGTTA+AGG	-	chr2.1:12426496-12426515	None:intergenic	35.0%
	TTATCTTGTTCTATACGAGG+TGG	-	chr2.1:12426822-12426841	None:intergenic	35.0%
	TTGTATTGCATCTAATGCTG+TGG	+	chr2.1:12427451-12427470	MS.gene35741:CDS	35.0%
!	GAGGTAAATTTGGAACAAGA+GGG	-	chr2.1:12426800-12426819	None:intergenic	35.0%
!	GTCTCATAAACAGCCAATTT+TGG	-	chr2.1:12427650-12427669	None:intergenic	35.0%
!	GTTTTGTATGATCAAATCGG+AGG	+	chr2.1:12427304-12427323	MS.gene35741:CDS	35.0%
!!	GCTGTTTATGAGACTGATTT+TGG	+	chr2.1:12427656-12427675	MS.gene35741:CDS	35.0%
!!	GTTGGTGATGGAACTAAATT+TGG	-	chr2.1:12426465-12426484	None:intergenic	35.0%
!!	TATGTAAGGAGGAGTGTTAT+GGG	-	chr2.1:12426679-12426698	None:intergenic	35.0%
!!	TTATGAGACTGATTTTGGGT+GGG	+	chr2.1:12427661-12427680	MS.gene35741:CDS	35.0%
!!	TTTATGAGACTGATTTTGGG+TGG	+	chr2.1:12427660-12427679	MS.gene35741:CDS	35.0%
	AAAGTTAAGGGGAGAATGGT+TGG	-	chr2.1:12426483-12426502	None:intergenic	40.0%
	AGCAAAAGTGATCAAGGTGA+AGG	+	chr2.1:12427335-12427354	MS.gene35741:CDS	40.0%
	AGGAGATAGAGTCTTCATCA+AGG	-	chr2.1:12426707-12426726	None:intergenic	40.0%
	ATACGAGGTGGAGGTAAATT+TGG	-	chr2.1:12426810-12426829	None:intergenic	40.0%
	CAGCAATATGGTTGTAACTG+AGG	-	chr2.1:12426911-12426930	None:intergenic	40.0%
	GAAGAAAGTTAAGGGGAGAA+TGG	-	chr2.1:12426487-12426506	None:intergenic	40.0%
	GAGTGTTATGGGAATTTGGA+GGG	-	chr2.1:12426668-12426687	None:intergenic	40.0%
	GGAAAGTGAGTGTTTGAGAA+TGG	-	chr2.1:12426601-12426620	None:intergenic	40.0%
	GGTGATAGCAAAAGTGATCA+AGG	+	chr2.1:12427329-12427348	MS.gene35741:CDS	40.0%
	GTTTGTAGGAAGTGATGAGT+TGG	-	chr2.1:12426570-12426589	None:intergenic	40.0%
	TACAACCATATTGCTGCAGA+CGG	+	chr2.1:12426915-12426934	MS.gene35741:CDS	40.0%
	TCAAGGTAACGTATGTAAGG+AGG	-	chr2.1:12426690-12426709	None:intergenic	40.0%
	TCATGTGAAGGAAGATCAAG+AGG	-	chr2.1:12427038-12427057	None:intergenic	40.0%
	TCTCTTTCTGATTGCAGAGA+TGG	+	chr2.1:12427734-12427753	MS.gene35741:CDS	40.0%
	TGTATGATCAAATCGGAGGA+GGG	+	chr2.1:12427308-12427327	MS.gene35741:CDS	40.0%
	TTGTATGATCAAATCGGAGG+AGG	+	chr2.1:12427307-12427326	MS.gene35741:CDS	40.0%
!	AGGTGGAATTGAGATTGGTT+TGG	+	chr2.1:12427760-12427779	MS.gene35741:CDS	40.0%
!	GATGGAACTAAATTTGGTGG+TGG	-	chr2.1:12426459-12426478	None:intergenic	40.0%
!	GGAGGTAAATTTGGAACAAG+AGG	-	chr2.1:12426801-12426820	None:intergenic	40.0%
!!	AAAGAAGTGTTGGAGGGTTA+AGG	-	chr2.1:12426622-12426641	None:intergenic	40.0%
!!	GAATGGGAAGTGTTGTTTGT+AGG	-	chr2.1:12426584-12426603	None:intergenic	40.0%
!!	GGTGATGGAACTAAATTTGG+TGG	-	chr2.1:12426462-12426481	None:intergenic	40.0%
!!	GTATGTAAGGAGGAGTGTTA+TGG	-	chr2.1:12426680-12426699	None:intergenic	40.0%
!!	TATGAGACTGATTTTGGGTG+GGG	+	chr2.1:12427662-12427681	MS.gene35741:CDS	40.0%
	ACAACCATATTGCTGCAGAC+GGG	+	chr2.1:12426916-12426935	MS.gene35741:CDS	45.0%
	ACTGAGGCAGATAGAGAAAC+CGG	-	chr2.1:12426895-12426914	None:intergenic	45.0%
	AGAAATGGAATCTGCGGGTT+TGG	+	chr2.1:12427130-12427149	MS.gene35741:CDS	45.0%
	ATGATGAGGTTGGAGGTGAA+GGG	-	chr2.1:12426645-12426664	None:intergenic	45.0%
	ATTTCCCGTCTGCAGCAATA+TGG	-	chr2.1:12426923-12426942	None:intergenic	45.0%
	ATTTGGAGGGATGATGAGGT+TGG	-	chr2.1:12426655-12426674	None:intergenic	45.0%
	CAGATAGAGAAACCGGAGTT+CGG	-	chr2.1:12426888-12426907	None:intergenic	45.0%
	CTTTCTGATTGCAGAGATGG+TGG	+	chr2.1:12427737-12427756	MS.gene35741:CDS	45.0%
	GAGAATGTTGAAGTCTGCTG+AGG	-	chr2.1:12426745-12426764	None:intergenic	45.0%
	GGGATTGTTGTGGCTTCAAA+TGG	+	chr2.1:12427506-12427525	MS.gene35741:CDS	45.0%
	TCTTGTTCTATACGAGGTGG+AGG	-	chr2.1:12426819-12426838	None:intergenic	45.0%
	TGTGAAGGAAGATCAAGAGG+CGG	-	chr2.1:12427035-12427054	None:intergenic	45.0%
!	AGGAAGTGATGAGTTGGATG+AGG	-	chr2.1:12426564-12426583	None:intergenic	45.0%
!	AGGTGAAGGGAAAGAAGTGT+TGG	-	chr2.1:12426632-12426651	None:intergenic	45.0%
!	GATGAGTTGGATGAGGAAAG+TGG	-	chr2.1:12426557-12426576	None:intergenic	45.0%
!	GGAGTGTTATGGGAATTTGG+AGG	-	chr2.1:12426669-12426688	None:intergenic	45.0%
!!	AGATTGGTTTGGCTCTTGAG+AGG	+	chr2.1:12427771-12427790	MS.gene35741:CDS	45.0%
!!	ATGAGACTGATTTTGGGTGG+GGG	+	chr2.1:12427663-12427682	MS.gene35741:CDS	45.0%
!!	GAAGGGAAAGAAGTGTTGGA+GGG	-	chr2.1:12426628-12426647	None:intergenic	45.0%
!!	GGAGGAGTGTTATGGGAATT+TGG	-	chr2.1:12426672-12426691	None:intergenic	45.0%
!!	GGTTGCTGGTTCACCAAAAT+TGG	+	chr2.1:12427634-12427653	MS.gene35741:CDS	45.0%
!!	TGAAGGGAAAGAAGTGTTGG+AGG	-	chr2.1:12426629-12426648	None:intergenic	45.0%
!!	TGCTATGGCTTTTGAAAGGG+AGG	-	chr2.1:12426973-12426992	None:intergenic	45.0%
	ACCACTACAAGAGACGTACC+CGG	-	chr2.1:12427617-12427636	None:intergenic	50.0%
	ACGTCTCTTGTAGTGGTTGC+TGG	+	chr2.1:12427620-12427639	MS.gene35741:CDS	50.0%
	GATGATGAGGTTGGAGGTGA+AGG	-	chr2.1:12426646-12426665	None:intergenic	50.0%
	GGGAATTTGGAGGGATGATG+AGG	-	chr2.1:12426659-12426678	None:intergenic	50.0%
	GGTGGAGGTGGAATTGAGAT+TGG	+	chr2.1:12427755-12427774	MS.gene35741:CDS	50.0%
	GTCACTGTTCTTCCGAACTC+CGG	+	chr2.1:12426873-12426892	MS.gene35741:CDS	50.0%
!	TCTGATTGCAGAGATGGTGG+AGG	+	chr2.1:12427740-12427759	MS.gene35741:CDS	50.0%
!!	AAGGGGAGAATGGTTGGTGA+TGG	-	chr2.1:12426477-12426496	None:intergenic	50.0%
	ACCGGGTACGTCTCTTGTAG+TGG	+	chr2.1:12427613-12427632	MS.gene35741:CDS	55.0%
	GTCTGCTGAGGATTCAGCGA+CGG	-	chr2.1:12426733-12426752	None:intergenic	55.0%
	TGAGGATTCAGCGACGGTGA+AGG	-	chr2.1:12426727-12426746	None:intergenic	55.0%
	TGGAGGGATGATGAGGTTGG+AGG	-	chr2.1:12426652-12426671	None:intergenic	55.0%
!!	GATTGCAGAGATGGTGGAGG+TGG	+	chr2.1:12427743-12427762	MS.gene35741:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	12426426	12427865	12426426	ID=MS.gene35741
chr2.1	mRNA	12426426	12427865	12426426	ID=MS.gene35741.t1;Parent=MS.gene35741
chr2.1	exon	12426426	12427865	12426426	ID=MS.gene35741.t1.exon1;Parent=MS.gene35741.t1
chr2.1	CDS	12426426	12427865	12426426	ID=cds.MS.gene35741.t1;Parent=MS.gene35741.t1

Gene Sequence

>MS.gene35741

ATGAAGGTGATAGAACATAGTCAAGTTGTACCACCACCAAATTTAGTTCCATCACCAACCATTCTCCCCTTAACTTTCTTCGACATACCATGGTTTTATTGTGACCCAATTCATCGTATCTTCTTCTACCACTTTCCTCATCCAACTCATCACTTCCTACAAACAACACTTCCCATTCTCAAACACTCACTTTCCTTAACCCTCCAACACTTCTTTCCCTTCACCTCCAACCTCATCATCCCTCCAAATTCCCATAACACTCCTCCTTACATACGTTACCTTGATGAAGACTCTATCTCCTTCACCGTCGCTGAATCCTCAGCAGACTTCAACATTCTCGTATCTGATTCACAAGATGCTCAAAACTGGCACCCTCTTGTTCCAAATTTACCTCCACCTCGTATAGAACAAGATAACATTCGTGTAATCCCTATAATGGCTATTCAAGTCACTGTTCTTCCGAACTCCGGTTTCTCTATCTGCCTCAGTTACAACCATATTGCTGCAGACGGGAAATCACTTCATCATTTCATGAAATTTTGGGCCTCCCTTTCAAAAGCCATAGCAAAAAACAATAACAAAAATAATTTGTCTCTTGAATACTCTCCGCCTCTTGATCTTCCTTCACATGAAAGAAATAGAGTTAAAGATCCAAAGGGTCTTAAACTTGTATACTTGCAACAATTAGAAGATTCTGTATCAAAAGAAATGGAATCTGCGGGTTTGGTTCGAGATTCTTATGTTAGCAAGGTACGAACAACTTTGATATTGAGCTATGAACAAGTCCAGAAGTTGAAGAAATGGGTTACTGATAAATGTAAAGATAGTGAAAGAATACAACACTTGTCTACTTTTGTTGTAACAAGTTCCTTGATTTGGTTTTGTATGATCAAATCGGAGGAGGGTGATAGCAAAAGTGATCAAGGTGAAGGTGATTGTGTTGTTGATGATAACTGCTACTTTGTGATTCTTGCAGATTGTCGTGATAGCGTTGAATTTTCATTACCAAAAACTTATTTTGGAAATTGTATTGCATCTAATGCTGTGGCTGTAAAGAGAGATGAGTTAGTTGGAAAAAATGGGATTGTTGTGGCTTCAAATGGTATTGAAAAGAAGATAAGAGATTTTAAGAATGATGCTTTGTTAGGAGCTGAATCGTTGATGCCTAATTATAGGAAATTCTCTAAACCGGGTACGTCTCTTGTAGTGGTTGCTGGTTCACCAAAATTGGCTGTTTATGAGACTGATTTTGGGTGGGGGAAGCCTAAGAAATCTGATGCAGTTCATCTTGATTCGTCCGTTTCGATTTCTCTTTCTGATTGCAGAGATGGTGGAGGTGGAATTGAGATTGGTTTGGCTCTTGAGAGGTCTAGAATGACTAATTTCATATACATCTTTCAAGAACGACTTGATAACATTTGTAGCATGCGAATGAACTAA

Protein sequence

>MS.gene35741.t1

MKVIEHSQVVPPPNLVPSPTILPLTFFDIPWFYCDPIHRIFFYHFPHPTHHFLQTTLPILKHSLSLTLQHFFPFTSNLIIPPNSHNTPPYIRYLDEDSISFTVAESSADFNILVSDSQDAQNWHPLVPNLPPPRIEQDNIRVIPIMAIQVTVLPNSGFSICLSYNHIAADGKSLHHFMKFWASLSKAIAKNNNKNNLSLEYSPPLDLPSHERNRVKDPKGLKLVYLQQLEDSVSKEMESAGLVRDSYVSKVRTTLILSYEQVQKLKKWVTDKCKDSERIQHLSTFVVTSSLIWFCMIKSEEGDSKSDQGEGDCVVDDNCYFVILADCRDSVEFSLPKTYFGNCIASNAVAVKRDELVGKNGIVVASNGIEKKIRDFKNDALLGAESLMPNYRKFSKPGTSLVVVAGSPKLAVYETDFGWGKPKKSDAVHLDSSVSISLSDCRDGGGGIEIGLALERSRMTNFIYIFQERLDNICSMRMN