AlfalfaGEDB | MS.gene063917

M. sativa cultivar XinJiangDaYe / MS.gene063917

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063917.t1	XP_003628087.1	98.3	60	1	0	1	60	1	60	5.70E-24	119.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063917.t1	G7LJ77	98.3	60	1	0	1	60	1	60	4.1e-24	119.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene063917	0.84034	9.22E-58	-1.69E-46
MS.gene050303	MS.gene063917	0.850594	1.54E-60	-1.69E-46
MS.gene050438	MS.gene063917	0.804358	2.24E-49	-1.69E-46
MS.gene050719	MS.gene063917	0.82773	1.33E-54	-1.69E-46
MS.gene050721	MS.gene063917	0.831083	2.04E-55	-1.69E-46
MS.gene050724	MS.gene063917	0.806941	6.44E-50	-1.69E-46
MS.gene050815	MS.gene063917	0.811868	5.65E-51	-1.69E-46
MS.gene051063	MS.gene063917	0.816085	6.63E-52	-1.69E-46
MS.gene051088	MS.gene063917	0.824804	6.62E-54	-1.69E-46
MS.gene051245	MS.gene063917	0.80972	1.65E-50	-1.69E-46
MS.gene051328	MS.gene063917	0.800682	1.29E-48	-1.69E-46
MS.gene051349	MS.gene063917	0.815737	7.93E-52	-1.69E-46
MS.gene051633	MS.gene063917	0.836431	9.40E-57	-1.69E-46
MS.gene051903	MS.gene063917	0.806396	8.40E-50	-1.69E-46
MS.gene052176	MS.gene063917	0.803979	2.69E-49	-1.69E-46
MS.gene052286	MS.gene063917	0.801553	8.53E-49	-1.69E-46
MS.gene052302	MS.gene063917	0.830822	2.37E-55	-1.69E-46
MS.gene052309	MS.gene063917	-0.809434	1.90E-50	-1.69E-46
MS.gene052376	MS.gene063917	0.841099	5.84E-58	-1.69E-46
MS.gene052947	MS.gene063917	0.801206	1.00E-48	-1.69E-46
MS.gene055808	MS.gene063917	0.804575	2.02E-49	-1.69E-46
MS.gene056091	MS.gene063917	0.815506	8.93E-52	-1.69E-46
MS.gene056631	MS.gene063917	0.853131	2.93E-61	-1.69E-46
MS.gene057079	MS.gene063917	0.815816	7.62E-52	-1.69E-46
MS.gene057140	MS.gene063917	0.839122	1.91E-57	-1.69E-46
MS.gene05795	MS.gene063917	0.817392	3.38E-52	-1.69E-46
MS.gene058084	MS.gene063917	0.845823	3.19E-59	-1.69E-46
MS.gene058238	MS.gene063917	0.8249	6.28E-54	-1.69E-46
MS.gene058906	MS.gene063917	0.868047	8.73E-66	-1.69E-46
MS.gene059249	MS.gene063917	0.822308	2.54E-53	-1.69E-46
MS.gene05954	MS.gene063917	0.827222	1.76E-54	-1.69E-46
MS.gene059569	MS.gene063917	0.805549	1.27E-49	-1.69E-46
MS.gene06001	MS.gene063917	0.844608	6.80E-59	-1.69E-46
MS.gene060429	MS.gene063917	0.829491	5.00E-55	-1.69E-46
MS.gene060572	MS.gene063917	0.821993	3.00E-53	-1.69E-46
MS.gene060586	MS.gene063917	0.832571	8.76E-56	-1.69E-46
MS.gene060907	MS.gene063917	0.821532	3.84E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063917.t1	MTR_8g043400	98.333	60	1	0	1	60	1	60	3.07e-38	120

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063917.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5
MS.gene063917.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5
MS.gene063917.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5
MS.gene063917.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5
MS.gene063917.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5

Find 20 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCTTTGGGCTTGTGCTAT+TGG	0.281471	8.1:-61404008	MS.gene063917:CDS
AGTGGTCTATGAGAGTTCTT+TGG	0.313525	8.1:-61404022	MS.gene063917:CDS
AGGCTTGAAAGGAATGGATT+TGG	0.349048	8.1:-61403584	MS.gene063917:CDS
ATCCATTCCTTTCAAGCCTT+CGG	0.357129	8.1:+61403588	None:intergenic
TAGAAAGACAAAAGCAGAAT+AGG	0.394330	8.1:-61403624	MS.gene063917:CDS
GTGGTCTATGAGAGTTCTTT+GGG	0.402171	8.1:-61404021	MS.gene063917:CDS
TACCTGTCTAGGTCTGTATA+TGG	0.414586	8.1:-61403653	MS.gene063917:intron
CTTGTCCGTAATTACTCCTC+TGG	0.492027	8.1:+61404043	None:intergenic
GGGAACCAGAGGAGTAATTA+CGG	0.516023	8.1:-61404048	MS.gene063917:CDS
CTTGAAAGGAATGGATTTGG+AGG	0.518401	8.1:-61403581	MS.gene063917:CDS
AAAGGAATGGATTTGGAGGA+AGG	0.535893	8.1:-61403577	MS.gene063917:CDS
GGCCGAAGGCTTGAAAGGAA+TGG	0.548731	8.1:-61403590	MS.gene063917:CDS
ATGTTGGCCGAAGGCTTGAA+AGG	0.560678	8.1:-61403595	MS.gene063917:CDS
GCAGAATAGGCAGAGGATGT+TGG	0.565240	8.1:-61403611	MS.gene063917:CDS
ATGGATTTGGAGGAAGGCAA+TGG	0.569370	8.1:-61403571	MS.gene063917:CDS
AATTACTCCTCTGGTTCCCA+TGG	0.583145	8.1:+61404052	None:intergenic
GACAAAAGCAGAATAGGCAG+AGG	0.586343	8.1:-61403618	MS.gene063917:CDS
CTGCATGCCATGGGAACCAG+AGG	0.620238	8.1:-61404059	None:intergenic
GAGGAGTAATTACGGACAAG+TGG	0.636089	8.1:-61404040	MS.gene063917:CDS
AGGCAGAGGATGTTGGCCGA+AGG	0.646645	8.1:-61403604	MS.gene063917:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGAAAGTTCCATTAATAA+AGG	+	chr8.1:61403652-61403671	None:intergenic	20.0%
!!	AAGAAAGTTCCATTAATAAA+GGG	+	chr8.1:61403651-61403670	None:intergenic	20.0%
!!!	TACATTTAAGTTTGTTAACA+AGG	+	chr8.1:61403682-61403701	None:intergenic	20.0%
!!	GGTAAACTATTTTCAAATAG+CGG	-	chr8.1:61403861-61403880	MS.gene063917:intron	25.0%
!!	GTTTTACGATACGTTATTTA+GGG	-	chr8.1:61403840-61403859	MS.gene063917:intron	25.0%
!!	TGTTTTACGATACGTTATTT+AGG	-	chr8.1:61403839-61403858	MS.gene063917:intron	25.0%
	CATTCTAGACCCTTTATTAA+TGG	-	chr8.1:61403639-61403658	MS.gene063917:CDS	30.0%
	TAACAGCTATCTAACAACAT+CGG	+	chr8.1:61403789-61403808	None:intergenic	30.0%
	TAGAAAGACAAAAGCAGAAT+AGG	-	chr8.1:61403984-61404003	MS.gene063917:intron	30.0%
!!	AAGTTTGTTAACAAGGCTTC+AGG	+	chr8.1:61403675-61403694	None:intergenic	35.0%
!!!	ACTATAGCACTTTTTCGTAG+TGG	-	chr8.1:61403894-61403913	MS.gene063917:intron	35.0%
	AACCATATACAGACCTAGAC+AGG	+	chr8.1:61403960-61403979	None:intergenic	40.0%
	ACACTGTACTATACCTGTCT+AGG	-	chr8.1:61403944-61403963	MS.gene063917:intron	40.0%
	AGATAGCTGTTATAGCGTAG+TGG	-	chr8.1:61403797-61403816	MS.gene063917:intron	40.0%
	AGTGGTCTATGAGAGTTCTT+TGG	-	chr8.1:61403586-61403605	MS.gene063917:CDS	40.0%
	ATCCATTCCTTTCAAGCCTT+CGG	+	chr8.1:61404023-61404042	None:intergenic	40.0%
	GTGGTCTATGAGAGTTCTTT+GGG	-	chr8.1:61403587-61403606	MS.gene063917:CDS	40.0%
	TACCTGTCTAGGTCTGTATA+TGG	-	chr8.1:61403955-61403974	MS.gene063917:intron	40.0%
!	AAAGGAATGGATTTGGAGGA+AGG	-	chr8.1:61404031-61404050	MS.gene063917:CDS	40.0%
!	AGGCTTGAAAGGAATGGATT+TGG	-	chr8.1:61404024-61404043	MS.gene063917:CDS	40.0%
!	CTTGAAAGGAATGGATTTGG+AGG	-	chr8.1:61404027-61404046	MS.gene063917:CDS	40.0%
	ATGGATTTGGAGGAAGGCAA+TGG	-	chr8.1:61404037-61404056	MS.gene063917:CDS	45.0%
	CTTGTCCGTAATTACTCCTC+TGG	+	chr8.1:61403568-61403587	None:intergenic	45.0%
	GACAAAAGCAGAATAGGCAG+AGG	-	chr8.1:61403990-61404009	MS.gene063917:intron	45.0%
	GAGGAGTAATTACGGACAAG+TGG	-	chr8.1:61403568-61403587	MS.gene063917:CDS	45.0%
	GGGAACCAGAGGAGTAATTA+CGG	-	chr8.1:61403560-61403579	MS.gene063917:CDS	45.0%
	GTTCTTTGGGCTTGTGCTAT+TGG	-	chr8.1:61403600-61403619	MS.gene063917:CDS	45.0%
	ATGTTGGCCGAAGGCTTGAA+AGG	-	chr8.1:61404013-61404032	MS.gene063917:CDS	50.0%
	GCAGAATAGGCAGAGGATGT+TGG	-	chr8.1:61403997-61404016	MS.gene063917:CDS	50.0%
	GGCCGAAGGCTTGAAAGGAA+TGG	-	chr8.1:61404018-61404037	MS.gene063917:CDS	55.0%
	AGGCAGAGGATGTTGGCCGA+AGG	-	chr8.1:61404004-61404023	MS.gene063917:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	61403558	61404072	61403558	ID=MS.gene063917
chr8.1	mRNA	61403558	61404072	61403558	ID=MS.gene063917.t1;Parent=MS.gene063917
chr8.1	exon	61403997	61404072	61403997	ID=MS.gene063917.t1.exon1;Parent=MS.gene063917.t1
chr8.1	CDS	61403997	61404072	61403997	ID=cds.MS.gene063917.t1;Parent=MS.gene063917.t1
chr8.1	exon	61403558	61403664	61403558	ID=MS.gene063917.t1.exon2;Parent=MS.gene063917.t1
chr8.1	CDS	61403558	61403664	61403558	ID=cds.MS.gene063917.t1;Parent=MS.gene063917.t1

Gene Sequence

>MS.gene063917

ATGGGAACCAGAGGAGTAATTACGGACAAGTGGTCTATGAGAGTTCTTTGGGCTTGTGCTATTGGAAGTGCTGTTAGTAAGCATTCTAGACCCTTTATTAATGGAACTTTCTTTCCTGAAGCCTTGTTAACAAACTTAAATGTAACTTTGTTATGTTATGTCTCTTCACTTTTTGTATAAAACATTGTTCATATGATCATATTTTTCTCATTATGAATCACTACTATACCGATGTTGTTAGATAGCTGTTATAGCGTAGTGGAACTCAAACAAATTGCTGTTGTTTTACGATACGTTATTTAGGGTAAACTATTTTCAAATAGCGGCTATAACAATACTATAGCACTTTTTCGTAGTGGAATTTCAACAACTGATATTTTCTGACGACACTGTACTATACCTGTCTAGGTCTGTATATGGTTGCTGTAGAAAGACAAAAGCAGAATAGGCAGAGGATGTTGGCCGAAGGCTTGAAAGGAATGGATTTGGAGGAAGGCAATGGTGAAAATGTTTGA

Protein sequence

>MS.gene063917.t1

MGTRGVITDKWSMRVLWACAIGSAVSLYMVAVERQKQNRQRMLAEGLKGMDLEEGNGENV