AlfalfaGEDB | MS.gene066063

M. sativa cultivar XinJiangDaYe / MS.gene066063

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066063.t1	XP_013469106.1	98.5	137	2	0	1	137	1	137	1.40E-62	248.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066063.t1	A0A072VMA2	98.5	137	2	0	1	137	1	137	1.0e-62	248.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050114	MS.gene066063	0.808959	2.40E-50	-1.69E-46
MS.gene050317	MS.gene066063	0.805589	1.24E-49	-1.69E-46
MS.gene050752	MS.gene066063	0.800223	1.59E-48	-1.69E-46
MS.gene050838	MS.gene066063	0.85996	2.88E-63	-1.69E-46
MS.gene051003	MS.gene066063	0.826863	2.15E-54	-1.69E-46
MS.gene051233	MS.gene066063	0.82475	6.81E-54	-1.69E-46
MS.gene051234	MS.gene066063	0.815029	1.14E-51	-1.69E-46
MS.gene051343	MS.gene066063	0.81698	4.18E-52	-1.69E-46
MS.gene051676	MS.gene066063	0.815659	8.25E-52	-1.69E-46
MS.gene051748	MS.gene066063	0.812867	3.42E-51	-1.69E-46
MS.gene051902	MS.gene066063	0.831402	1.70E-55	-1.69E-46
MS.gene051903	MS.gene066063	0.802248	6.14E-49	-1.69E-46
MS.gene051975	MS.gene066063	0.80548	1.31E-49	-1.69E-46
MS.gene052342	MS.gene066063	0.812502	4.11E-51	-1.69E-46
MS.gene052434	MS.gene066063	0.824801	6.63E-54	-1.69E-46
MS.gene052818	MS.gene066063	-0.805605	1.23E-49	-1.69E-46
MS.gene052947	MS.gene066063	0.813537	2.43E-51	-1.69E-46
MS.gene053058	MS.gene066063	0.824165	9.35E-54	-1.69E-46
MS.gene053298	MS.gene066063	0.805474	1.31E-49	-1.69E-46
MS.gene053564	MS.gene066063	0.819047	1.43E-52	-1.69E-46
MS.gene053786	MS.gene066063	0.842693	2.21E-58	-1.69E-46
MS.gene054403	MS.gene066063	0.800635	1.31E-48	-1.69E-46
MS.gene055311	MS.gene066063	0.82637	2.82E-54	-1.69E-46
MS.gene055313	MS.gene066063	0.810886	9.22E-51	-1.69E-46
MS.gene055314	MS.gene066063	0.814387	1.58E-51	-1.69E-46
MS.gene055513	MS.gene066063	0.81633	5.85E-52	-1.69E-46
MS.gene056181	MS.gene066063	0.827686	1.36E-54	-1.69E-46
MS.gene056390	MS.gene066063	0.800308	1.53E-48	-1.69E-46
MS.gene056403	MS.gene066063	0.804986	1.66E-49	-1.69E-46
MS.gene056752	MS.gene066063	0.82029	7.42E-53	-1.69E-46
MS.gene056986	MS.gene066063	0.814734	1.32E-51	-1.69E-46
MS.gene05781	MS.gene066063	0.816252	6.09E-52	-1.69E-46
MS.gene057890	MS.gene066063	-0.810375	1.19E-50	-1.69E-46
MS.gene057999	MS.gene066063	0.802952	4.39E-49	-1.69E-46
MS.gene058074	MS.gene066063	0.809939	1.48E-50	-1.69E-46
MS.gene058639	MS.gene066063	0.810832	9.48E-51	-1.69E-46
MS.gene058654	MS.gene066063	0.807965	3.91E-50	-1.69E-46
MS.gene059171	MS.gene066063	0.823453	1.37E-53	-1.69E-46
MS.gene059227	MS.gene066063	0.800316	1.53E-48	-1.69E-46
MS.gene06011	MS.gene066063	0.832743	7.94E-56	-1.69E-46
MS.gene060763	MS.gene066063	0.824154	9.41E-54	-1.69E-46
MS.gene060869	MS.gene066063	0.800941	1.14E-48	-1.69E-46
MS.gene061100	MS.gene066063	0.811769	5.93E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066063.t1	MTR_1g085355	98.540	137	2	0	1	137	1	137	3.13e-94	268
MS.gene066063.t1	MTR_4g118050	97.810	137	2	1	1	137	1	136	1.70e-91	261
MS.gene066063.t1	MTR_1g085440	94.161	137	7	1	1	137	1	136	7.95e-89	254

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066063.t1	AT5G03050	58.730	126	47	2	10	132	11	134	1.44e-42	137
MS.gene066063.t1	AT5G03050	58.730	126	47	2	10	132	11	134	1.44e-42	137

Find 22 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCAGGGAAAACAATGTTTA+AGG	0.261813	1.2:-58609977	MS.gene066063:CDS
GTATCTGAGCTCTTGGAATC+AGG	0.345471	1.2:-58609994	MS.gene066063:CDS
CCAAGTTGGGTCTGAAGAAA+TGG	0.369614	1.2:-58610167	MS.gene066063:CDS
AGCATTAGCTTCCTCATTTG+AGG	0.397998	1.2:+58609747	None:intergenic
TAACCAATGTCAGCTCAAAA+AGG	0.399910	1.2:-58610408	None:intergenic
CTTGTAGGTATCTGAGCTCT+TGG	0.437315	1.2:-58610001	MS.gene066063:intron
CCATTTCTTCAGACCCAACT+TGG	0.441813	1.2:+58610167	None:intergenic
AAGCAGGATTCACAAGGAAC+AGG	0.472958	1.2:-58609815	MS.gene066063:intron
GGTTTGAGACTAACCAAGTT+GGG	0.494830	1.2:-58610180	MS.gene066063:CDS
TATCTGAGCTCTTGGAATCA+GGG	0.503680	1.2:-58609993	MS.gene066063:CDS
GCATGGACAGTGGAGCTTCA+AGG	0.522029	1.2:-58610383	MS.gene066063:CDS
TCAAGGAAACGTATGAAACC+TGG	0.524964	1.2:-58610366	MS.gene066063:CDS
ATCGCAGATTGTCCTTGTAC+AGG	0.525946	1.2:-58610209	MS.gene066063:CDS
CAAACCCTTCCTCCTGTACA+AGG	0.529759	1.2:+58610197	None:intergenic
ATTGTCCTTGTACAGGAGGA+AGG	0.538219	1.2:-58610202	MS.gene066063:CDS
TTGTCCTTGTACAGGAGGAA+GGG	0.558464	1.2:-58610201	MS.gene066063:CDS
CAAGGAAACGTATGAAACCT+GGG	0.567520	1.2:-58610365	MS.gene066063:CDS
TCAAATAAGCAGGATTCACA+AGG	0.592512	1.2:-58609821	MS.gene066063:intron
GGGTTTGAGACTAACCAAGT+TGG	0.597415	1.2:-58610181	MS.gene066063:CDS
TGCACTTGATGCCTCAAATG+AGG	0.607261	1.2:-58609758	MS.gene066063:CDS
GCAGATTGTCCTTGTACAGG+AGG	0.643938	1.2:-58610206	MS.gene066063:CDS
AAGGAAACGTATGAAACCTG+GGG	0.689052	1.2:-58610364	MS.gene066063:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTAAACATTGAATGTTCAA+TGG	+	chr1.2:58610200-58610219	None:intergenic	20.0%
!!!	ATGTTTGAAATGTTTAATTG+TGG	-	chr1.2:58610073-58610092	MS.gene066063:intron	20.0%
!!!	TGTTAGTTTTTGACTTTTTA+TGG	-	chr1.2:58610240-58610259	MS.gene066063:CDS	20.0%
!	TTTAATTGTGGATTTCTTGT+AGG	-	chr1.2:58610085-58610104	MS.gene066063:intron	25.0%
!!	CTTTTCTTTTCACAATAAAC+AGG	-	chr1.2:58609838-58609857	MS.gene066063:intron	25.0%
!!!	CTTTTAACTTTCAAATAAGC+AGG	-	chr1.2:58610270-58610289	MS.gene066063:intron	25.0%
	ATCAGGGAAAACAATGTTTA+AGG	-	chr1.2:58610124-58610143	MS.gene066063:CDS	30.0%
	ATGACGAGAAAAATGTTGAA+AGG	+	chr1.2:58609778-58609797	None:intergenic	30.0%
!	AGATTGAGAATTTTACTCAG+AGG	-	chr1.2:58609984-58610003	MS.gene066063:CDS	30.0%
!	GATTGAGAATTTTACTCAGA+GGG	-	chr1.2:58609985-58610004	MS.gene066063:CDS	30.0%
	TCAAATAAGCAGGATTCACA+AGG	-	chr1.2:58610280-58610299	MS.gene066063:intron	35.0%
!	ACAAGGAACAGGTTGAAAAA+TGG	-	chr1.2:58610297-58610316	MS.gene066063:intron	35.0%
	AAGGAAACGTATGAAACCTG+GGG	-	chr1.2:58609737-58609756	MS.gene066063:CDS	40.0%
	CAAGGAAACGTATGAAACCT+GGG	-	chr1.2:58609736-58609755	MS.gene066063:CDS	40.0%
	GCGAAAATAACAATTACCCC+AGG	+	chr1.2:58609756-58609775	None:intergenic	40.0%
	TATCTGAGCTCTTGGAATCA+GGG	-	chr1.2:58610108-58610127	MS.gene066063:intron	40.0%
	TCAAGGAAACGTATGAAACC+TGG	-	chr1.2:58609735-58609754	MS.gene066063:CDS	40.0%
!	AGCATTAGCTTCCTCATTTG+AGG	+	chr1.2:58610357-58610376	None:intergenic	40.0%
!	GGTTTGAGACTAACCAAGTT+GGG	-	chr1.2:58609921-58609940	MS.gene066063:intron	40.0%
	AAGCAGGATTCACAAGGAAC+AGG	-	chr1.2:58610286-58610305	MS.gene066063:intron	45.0%
	ATCGCAGATTGTCCTTGTAC+AGG	-	chr1.2:58609892-58609911	MS.gene066063:intron	45.0%
	ATTGTCCTTGTACAGGAGGA+AGG	-	chr1.2:58609899-58609918	MS.gene066063:intron	45.0%
	CCATTTCTTCAGACCCAACT+TGG	+	chr1.2:58609937-58609956	None:intergenic	45.0%
	CTTGTAGGTATCTGAGCTCT+TGG	-	chr1.2:58610100-58610119	MS.gene066063:intron	45.0%
	GTATCTGAGCTCTTGGAATC+AGG	-	chr1.2:58610107-58610126	MS.gene066063:intron	45.0%
	GTCAGCTCAAAAAGGAAGCA+TGG	-	chr1.2:58609701-58609720	MS.gene066063:CDS	45.0%
	TGCACTTGATGCCTCAAATG+AGG	-	chr1.2:58610343-58610362	MS.gene066063:intron	45.0%
	TTGTCCTTGTACAGGAGGAA+GGG	-	chr1.2:58609900-58609919	MS.gene066063:intron	45.0%
!	CAAAAAGGAAGCATGGACAG+TGG	-	chr1.2:58609708-58609727	MS.gene066063:CDS	45.0%
!	CCAAGTTGGGTCTGAAGAAA+TGG	-	chr1.2:58609934-58609953	MS.gene066063:intron	45.0%
!	GGAACAGGTTGAAAAATGGC+AGG	-	chr1.2:58610301-58610320	MS.gene066063:intron	45.0%
!	GGGTTTGAGACTAACCAAGT+TGG	-	chr1.2:58609920-58609939	MS.gene066063:intron	45.0%
	CAAACCCTTCCTCCTGTACA+AGG	+	chr1.2:58609907-58609926	None:intergenic	50.0%
	GCAGATTGTCCTTGTACAGG+AGG	-	chr1.2:58609895-58609914	MS.gene066063:intron	50.0%
	GCATGGACAGTGGAGCTTCA+AGG	-	chr1.2:58609718-58609737	MS.gene066063:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	58609699	58610424	58609699	ID=MS.gene066063
chr1.2	mRNA	58609699	58610424	58609699	ID=MS.gene066063.t1;Parent=MS.gene066063
chr1.2	exon	58610365	58610424	58610365	ID=MS.gene066063.t1.exon1;Parent=MS.gene066063.t1
chr1.2	CDS	58610365	58610424	58610365	ID=cds.MS.gene066063.t1;Parent=MS.gene066063.t1
chr1.2	exon	58610117	58610263	58610117	ID=MS.gene066063.t1.exon2;Parent=MS.gene066063.t1
chr1.2	CDS	58610117	58610263	58610117	ID=cds.MS.gene066063.t1;Parent=MS.gene066063.t1
chr1.2	exon	58609943	58610016	58609943	ID=MS.gene066063.t1.exon3;Parent=MS.gene066063.t1
chr1.2	CDS	58609943	58610016	58609943	ID=cds.MS.gene066063.t1;Parent=MS.gene066063.t1
chr1.2	exon	58609699	58609831	58609699	ID=MS.gene066063.t1.exon4;Parent=MS.gene066063.t1
chr1.2	CDS	58609699	58609831	58609699	ID=cds.MS.gene066063.t1;Parent=MS.gene066063.t1

Gene Sequence

>MS.gene066063

ATGTCAGCTCAAAAAGGAAGCATGGACAGTGGAGCTTCAAGGAAACGTATGAAACCTGGGGTAATTGTTATTTTCGCCTTTCAACATTTTTCTCGTCATGAATTTACAGCATTTATAGCATTCTTTGTTGTTTCATTTGCTTTTCTTTTCACAATAAACAGGTAGTACAAGAAAAGATTAGAGATGAAGCAGAATCGCAGATTGTCCTTGTACAGGAGGAAGGGTTTGAGACTAACCAAGTTGGGTCTGAAGAAATGGAGCTCAATATTTCTCTTGTTCTTGAGAAGATTGAGAATTTTACTCAGAGGGTAGCTATAAAAAGTTACACTTACACACTGTTTGAAGCTTGAAATCATACTCTAGATGAATGTTAAATGTTTGAAATGTTTAATTGTGGATTTCTTGTAGGTATCTGAGCTCTTGGAATCAGGGAAAACAATGTTTAAGGAGCTATGCAATGAATTTGAAGAGAAACTGATTATGTGAGTTATGAAGTTACCATTGAACATTCAATGTTTAAACTATCTTTCACTTTAAATATTGTTAGTTTTTGACTTTTTATGGTGTGATTCTTTTAACTTTCAAATAAGCAGGATTCACAAGGAACAGGTTGAAAAATGGCAGGAAGAGATCAAAGAACTGCGTGCACTTGATGCCTCAAATGAGGAAGCTAATGCTCTTCTGCAAAATGCACGATATGTACTTCAGCTAACTCGTAATGATTAG

Protein sequence

>MS.gene066063.t1

MSAQKGSMDSGASRKRMKPGVVQEKIRDEAESQIVLVQEEGFETNQVGSEEMELNISLVLEKIENFTQRVSELLESGKTMFKELCNEFEEKLIMIHKEQVEKWQEEIKELRALDASNEEANALLQNARYVLQLTRND