Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067305.t1 | RHN39669.1 | 96.8 | 252 | 8 | 0 | 1 | 252 | 1 | 252 | 3.50E-131 | 477.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067305.t1 | G7LAU0 | 96.8 | 252 | 8 | 0 | 1 | 252 | 25 | 276 | 2.5e-131 | 477.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene067305 | 0.81171 | 6.11E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene067305 | 0.884839 | 1.36E-71 | -1.69E-46 |
| MS.gene05016 | MS.gene067305 | 0.814495 | 1.50E-51 | -1.69E-46 |
| MS.gene050206 | MS.gene067305 | 0.841954 | 3.47E-58 | -1.69E-46 |
| MS.gene050563 | MS.gene067305 | 0.835903 | 1.28E-56 | -1.69E-46 |
| MS.gene050584 | MS.gene067305 | 0.878112 | 3.65E-69 | -1.69E-46 |
| MS.gene050585 | MS.gene067305 | 0.850863 | 1.29E-60 | -1.69E-46 |
| MS.gene050586 | MS.gene067305 | 0.84074 | 7.25E-58 | -1.69E-46 |
| MS.gene050587 | MS.gene067305 | 0.807837 | 4.16E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene067305 | 0.878984 | 1.80E-69 | -1.69E-46 |
| MS.gene050808 | MS.gene067305 | 0.85245 | 4.58E-61 | -1.69E-46 |
| MS.gene05143 | MS.gene067305 | 0.813065 | 3.09E-51 | -1.69E-46 |
| MS.gene051626 | MS.gene067305 | 0.846928 | 1.60E-59 | -1.69E-46 |
| MS.gene051789 | MS.gene067305 | 0.907039 | 7.79E-81 | -1.69E-46 |
| MS.gene052142 | MS.gene067305 | 0.842932 | 1.91E-58 | -1.69E-46 |
| MS.gene052665 | MS.gene067305 | 0.820794 | 5.68E-53 | -1.69E-46 |
| MS.gene052967 | MS.gene067305 | 0.818172 | 2.25E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene067305 | 0.90563 | 3.50E-80 | -1.69E-46 |
| MS.gene053184 | MS.gene067305 | 0.80739 | 5.18E-50 | -1.69E-46 |
| MS.gene053204 | MS.gene067305 | 0.821463 | 3.98E-53 | -1.69E-46 |
| MS.gene053383 | MS.gene067305 | 0.870017 | 2.00E-66 | -1.69E-46 |
| MS.gene053563 | MS.gene067305 | 0.846194 | 2.53E-59 | -1.69E-46 |
| MS.gene053630 | MS.gene067305 | 0.816036 | 6.80E-52 | -1.69E-46 |
| MS.gene054184 | MS.gene067305 | 0.806057 | 9.89E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene067305 | 0.808929 | 2.43E-50 | -1.69E-46 |
| MS.gene05456 | MS.gene067305 | 0.875497 | 2.94E-68 | -1.69E-46 |
| MS.gene054596 | MS.gene067305 | 0.851115 | 1.09E-60 | -1.69E-46 |
| MS.gene055207 | MS.gene067305 | 0.819537 | 1.10E-52 | -1.69E-46 |
| MS.gene055358 | MS.gene067305 | 0.814225 | 1.72E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene067305 | 0.858881 | 6.08E-63 | -1.69E-46 |
| MS.gene056069 | MS.gene067305 | -0.816046 | 6.77E-52 | -1.69E-46 |
| MS.gene056760 | MS.gene067305 | 0.810383 | 1.18E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene067305 | 0.816068 | 6.69E-52 | -1.69E-46 |
| MS.gene057395 | MS.gene067305 | 0.811015 | 8.65E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene067305 | 0.835783 | 1.37E-56 | -1.69E-46 |
| MS.gene059006 | MS.gene067305 | 0.913098 | 9.12E-84 | -1.69E-46 |
| MS.gene059124 | MS.gene067305 | 0.818527 | 1.87E-52 | -1.69E-46 |
| MS.gene060470 | MS.gene067305 | 0.811344 | 7.34E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene067305 | 0.870278 | 1.65E-66 | -1.69E-46 |
| MS.gene060691 | MS.gene067305 | 0.802246 | 6.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067305.t1 | MTR_8g027100 | 96.825 | 252 | 8 | 0 | 1 | 252 | 25 | 276 | 4.02e-180 | 495 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067305.t1 | AT2G46640 | 30.736 | 231 | 125 | 9 | 12 | 226 | 38 | 249 | 4.78e-15 | 73.2 |
| MS.gene067305.t1 | AT2G46640 | 30.736 | 231 | 125 | 9 | 12 | 226 | 40 | 251 | 5.23e-15 | 73.2 |
| MS.gene067305.t1 | AT2G46640 | 30.736 | 231 | 125 | 9 | 12 | 226 | 26 | 237 | 5.35e-15 | 73.2 |
| MS.gene067305.t1 | AT2G46640 | 30.736 | 231 | 125 | 9 | 12 | 226 | 40 | 251 | 6.52e-15 | 73.2 |
Find 36 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCAAAGCAAAGTATTCT+TGG | 0.268337 | 8.2:-70902309 | None:intergenic |
| GTATTCTTGGTGATTGTTCA+TGG | 0.327683 | 8.2:-70902296 | None:intergenic |
| GAAGATTATTCCCCTTGTTA+AGG | 0.356621 | 8.2:+70902735 | MS.gene067305:CDS |
| AATGATGATTAGAAACTTCA+AGG | 0.418804 | 8.2:+70902504 | MS.gene067305:CDS |
| TCACCAAGAATACTTTGCTT+TGG | 0.419615 | 8.2:+70902306 | MS.gene067305:CDS |
| ATTCTTACCATTGGTACTCT+TGG | 0.429438 | 8.2:+70902256 | MS.gene067305:CDS |
| ACTTGACTCAACCAAGACTT+TGG | 0.432800 | 8.2:-70902668 | None:intergenic |
| ACTTTCTTGATGTCTTTCAT+TGG | 0.437252 | 8.2:-70902769 | None:intergenic |
| ATGATTTCATGACAAATCAT+AGG | 0.438088 | 8.2:-70902544 | None:intergenic |
| TCAAATGTGTTGTTGATCAA+TGG | 0.438577 | 8.2:-70902409 | None:intergenic |
| TTTCCAATCACCGAGCATAT+CGG | 0.445673 | 8.2:-70902227 | None:intergenic |
| TGACCGATATGCTCGGTGAT+TGG | 0.446300 | 8.2:+70902224 | MS.gene067305:CDS |
| TGATTCTTGACATCAAGATC+TGG | 0.455218 | 8.2:-70903133 | None:intergenic |
| ATTTGATGAGAACAACTTTG+AGG | 0.478699 | 8.2:+70902426 | MS.gene067305:CDS |
| TTATCAGTGTTGTTGATTGA+AGG | 0.480019 | 8.2:-70902175 | None:intergenic |
| ATTAATAACCACATGAATGA+AGG | 0.483710 | 8.2:+70903175 | MS.gene067305:CDS |
| TGATGAAGAAGATGCTAAAG+AGG | 0.486614 | 8.2:+70903101 | MS.gene067305:CDS |
| TATGATTTGTCATGAAATCA+TGG | 0.489589 | 8.2:+70902546 | MS.gene067305:CDS |
| TTGAAGCTGATCAAAAGAAA+AGG | 0.490869 | 8.2:+70902581 | MS.gene067305:CDS |
| TGATGATGATGCTAGAAAAG+AGG | 0.505541 | 8.2:+70902477 | MS.gene067305:CDS |
| TTATTCTGCAGCCAAAGTCT+TGG | 0.511362 | 8.2:+70902657 | MS.gene067305:CDS |
| TTTCTATGATGTTGAACAAG+AGG | 0.511917 | 8.2:+70902378 | MS.gene067305:CDS |
| AGCCATTGCCTTCATTCATG+TGG | 0.516778 | 8.2:-70903183 | None:intergenic |
| AAAGACGGAATTCTTACCAT+TGG | 0.543523 | 8.2:+70902247 | MS.gene067305:CDS |
| AAACTTCAAGGAAATTGTTG+AGG | 0.544282 | 8.2:+70902516 | MS.gene067305:CDS |
| CTTCAAGGAAATTGTTGAGG+TGG | 0.564269 | 8.2:+70902519 | MS.gene067305:CDS |
| ATCATAGCCAAGAGTACCAA+TGG | 0.569824 | 8.2:-70902263 | None:intergenic |
| CATCATCATCAACTACATCA+TGG | 0.574853 | 8.2:-70902465 | None:intergenic |
| ATGCTCGGTGATTGGAAAGA+CGG | 0.581029 | 8.2:+70902232 | MS.gene067305:CDS |
| TCTTGACATCAAGATCTGGA+TGG | 0.589109 | 8.2:-70903129 | None:intergenic |
| TGAATGAAGGCAATGGCTCA+AGG | 0.597220 | 8.2:+70903188 | MS.gene067305:CDS |
| TGATTGTTCATGGATTTCAA+AGG | 0.604206 | 8.2:-70902286 | None:intergenic |
| AACCACATGAATGAAGGCAA+TGG | 0.612697 | 8.2:+70903181 | MS.gene067305:CDS |
| GTTGCTTTGACCGATATGCT+CGG | 0.632441 | 8.2:+70902217 | MS.gene067305:CDS |
| TCTTAACATCAGAATCAGCA+AGG | 0.653265 | 8.2:-70902624 | None:intergenic |
| GATTTGTCATGAAATCATGG+AGG | 0.748556 | 8.2:+70902549 | MS.gene067305:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTTGCTTGAAATTAAT+TGG | + | chr8.2:70903048-70903067 | MS.gene067305:intron | 15.0% |
| !! | TCAAGCAAATAATTAATTAT+TGG | - | chr8.2:70903041-70903060 | None:intergenic | 15.0% |
| !! | TAATTACATATATAGCCTTT+AGG | - | chr8.2:70902821-70902840 | None:intergenic | 20.0% |
| !!! | ATTTATTTCCATTTTCACAA+TGG | + | chr8.2:70902925-70902944 | MS.gene067305:intron | 20.0% |
| ! | AATGATGATTAGAAACTTCA+AGG | + | chr8.2:70902504-70902523 | MS.gene067305:CDS | 25.0% |
| ! | ATGATTTCATGACAAATCAT+AGG | - | chr8.2:70902547-70902566 | None:intergenic | 25.0% |
| ! | ATTAATAACCACATGAATGA+AGG | + | chr8.2:70903175-70903194 | MS.gene067305:CDS | 25.0% |
| ! | CCAAAACTTAAAGCAAAAAA+TGG | + | chr8.2:70902697-70902716 | MS.gene067305:CDS | 25.0% |
| ! | TTGCTTGAAATTAATTGGTT+AGG | + | chr8.2:70903053-70903072 | MS.gene067305:intron | 25.0% |
| !! | TATGATTTGTCATGAAATCA+TGG | + | chr8.2:70902546-70902565 | MS.gene067305:CDS | 25.0% |
| !!! | CCATTTTTTGCTTTAAGTTT+TGG | - | chr8.2:70902700-70902719 | None:intergenic | 25.0% |
| AAACTTCAAGGAAATTGTTG+AGG | + | chr8.2:70902516-70902535 | MS.gene067305:CDS | 30.0% | |
| ACTTTCTTGATGTCTTTCAT+TGG | - | chr8.2:70902772-70902791 | None:intergenic | 30.0% | |
| ATTTGATGAGAACAACTTTG+AGG | + | chr8.2:70902426-70902445 | MS.gene067305:CDS | 30.0% | |
| TGATTGTTCATGGATTTCAA+AGG | - | chr8.2:70902289-70902308 | None:intergenic | 30.0% | |
| TTGAAGCTGATCAAAAGAAA+AGG | + | chr8.2:70902581-70902600 | MS.gene067305:CDS | 30.0% | |
| TTTCTATGATGTTGAACAAG+AGG | + | chr8.2:70902378-70902397 | MS.gene067305:CDS | 30.0% | |
| ! | TCAAATGTGTTGTTGATCAA+TGG | - | chr8.2:70902412-70902431 | None:intergenic | 30.0% |
| ! | TTATCAGTGTTGTTGATTGA+AGG | - | chr8.2:70902178-70902197 | None:intergenic | 30.0% |
| AAAGACGGAATTCTTACCAT+TGG | + | chr8.2:70902247-70902266 | MS.gene067305:CDS | 35.0% | |
| ATTCTTACCATTGGTACTCT+TGG | + | chr8.2:70902256-70902275 | MS.gene067305:CDS | 35.0% | |
| CATCATCATCAACTACATCA+TGG | - | chr8.2:70902468-70902487 | None:intergenic | 35.0% | |
| CATTGACACCATTGTGAAAA+TGG | - | chr8.2:70902936-70902955 | None:intergenic | 35.0% | |
| GAAGATTATTCCCCTTGTTA+AGG | + | chr8.2:70902735-70902754 | MS.gene067305:CDS | 35.0% | |
| GATTTGTCATGAAATCATGG+AGG | + | chr8.2:70902549-70902568 | MS.gene067305:CDS | 35.0% | |
| TCACCAAGAATACTTTGCTT+TGG | + | chr8.2:70902306-70902325 | MS.gene067305:CDS | 35.0% | |
| TCTTAACATCAGAATCAGCA+AGG | - | chr8.2:70902627-70902646 | None:intergenic | 35.0% | |
| TGATGAAGAAGATGCTAAAG+AGG | + | chr8.2:70903101-70903120 | MS.gene067305:CDS | 35.0% | |
| TGATTCTTGACATCAAGATC+TGG | - | chr8.2:70903136-70903155 | None:intergenic | 35.0% | |
| TTGTAGATAGGATAGTGTTG+AGG | + | chr8.2:70903015-70903034 | MS.gene067305:intron | 35.0% | |
| ! | ACGTGCATTTTCCTTAACAA+GGG | - | chr8.2:70902749-70902768 | None:intergenic | 35.0% |
| ! | CTTCCAAAGCAAAGTATTCT+TGG | - | chr8.2:70902312-70902331 | None:intergenic | 35.0% |
| ! | GTATTCTTGGTGATTGTTCA+TGG | - | chr8.2:70902299-70902318 | None:intergenic | 35.0% |
| ! | TGATGATGATGCTAGAAAAG+AGG | + | chr8.2:70902477-70902496 | MS.gene067305:CDS | 35.0% |
| AACCACATGAATGAAGGCAA+TGG | + | chr8.2:70903181-70903200 | MS.gene067305:CDS | 40.0% | |
| ACTTGACTCAACCAAGACTT+TGG | - | chr8.2:70902671-70902690 | None:intergenic | 40.0% | |
| ATCTACAAAAGAGTCCTCAC+TGG | - | chr8.2:70903003-70903022 | None:intergenic | 40.0% | |
| CTACATCATGCACTTCCTAA+AGG | + | chr8.2:70902803-70902822 | MS.gene067305:intron | 40.0% | |
| CTTCAAGGAAATTGTTGAGG+TGG | + | chr8.2:70902519-70902538 | MS.gene067305:CDS | 40.0% | |
| TCTTGACATCAAGATCTGGA+TGG | - | chr8.2:70903132-70903151 | None:intergenic | 40.0% | |
| TTATTCTGCAGCCAAAGTCT+TGG | + | chr8.2:70902657-70902676 | MS.gene067305:CDS | 40.0% | |
| TTTCCAATCACCGAGCATAT+CGG | - | chr8.2:70902230-70902249 | None:intergenic | 40.0% | |
| ! | CGTGCATTTTCCTTAACAAG+GGG | - | chr8.2:70902748-70902767 | None:intergenic | 40.0% |
| ! | GACGTGCATTTTCCTTAACA+AGG | - | chr8.2:70902750-70902769 | None:intergenic | 40.0% |
| ! | GTGAGGACTCTTTTGTAGAT+AGG | + | chr8.2:70903003-70903022 | MS.gene067305:intron | 40.0% |
| !! | ATCATAGCCAAGAGTACCAA+TGG | - | chr8.2:70902266-70902285 | None:intergenic | 40.0% |
| AGCCATTGCCTTCATTCATG+TGG | - | chr8.2:70903186-70903205 | None:intergenic | 45.0% | |
| GCTTCATAGTTTGACCAGTG+AGG | + | chr8.2:70902986-70903005 | MS.gene067305:intron | 45.0% | |
| TGAATGAAGGCAATGGCTCA+AGG | + | chr8.2:70903188-70903207 | MS.gene067305:CDS | 45.0% | |
| ! | ATGCTCGGTGATTGGAAAGA+CGG | + | chr8.2:70902232-70902251 | MS.gene067305:CDS | 45.0% |
| ! | GTTGCTTTGACCGATATGCT+CGG | + | chr8.2:70902217-70902236 | MS.gene067305:CDS | 45.0% |
| ! | TGACCGATATGCTCGGTGAT+TGG | + | chr8.2:70902224-70902243 | MS.gene067305:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 70902160 | 70903228 | 70902160 | ID=MS.gene067305 |
| chr8.2 | mRNA | 70902160 | 70903228 | 70902160 | ID=MS.gene067305.t1;Parent=MS.gene067305 |
| chr8.2 | exon | 70902160 | 70902789 | 70902160 | ID=MS.gene067305.t1.exon1;Parent=MS.gene067305.t1 |
| chr8.2 | CDS | 70902160 | 70902789 | 70902160 | ID=cds.MS.gene067305.t1;Parent=MS.gene067305.t1 |
| chr8.2 | exon | 70903100 | 70903228 | 70903100 | ID=MS.gene067305.t1.exon2;Parent=MS.gene067305.t1 |
| chr8.2 | CDS | 70903100 | 70903228 | 70903100 | ID=cds.MS.gene067305.t1;Parent=MS.gene067305.t1 |
| Gene Sequence |
| Protein sequence |
